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Heavy metal contamination of water sources has long been a silent yet potent threat, endangering environmental and human health. Conventional wastewater treatments are costly due to high infrastructure expenses, energy consumption, and chemical usage. These treatments lead to secondary environmental pollution, such as producing toxic sludge, greenhouse gaseous emissions, and residual pollutants discharges. Therefore, more sustainable and cost-effective wastewater treatment alternatives are needed to overcome these challenges. Microalgae biosorption and bioaccumulation can bioremediate wastewater by effectively removing heavy metals and other contaminants, such as nitrate and phosphorus. By utilizing sunlight and CO 2 for growth, microalgae cultivation reduces the need for expensive chemicals and energy-intensive operations in wastewater treatment. Additionally, microalgae can potentially convert heavy metal ions from wastewater into metal nanoparticles, providing a dual benefit of bioremediation and resource recovery. The primary objectives of this review are to assess the effectiveness of microalgae in heavy metal bioremediation and nanoparticle synthesis while also identifying critical research gaps and future directions for optimizing this biotechnology. Heavy metal ions in wastewater can be used as a metal precursor, and metal nanoparticles can be synthesized from wastewater. A review methodology was carried out to assess the availability of literature for readers to identify the research trends and gaps. Mechanisms of microalgae for the biogenesis of metal nanoparticles, including activation, growth, and termination phases, were elucidated. Various chemical interactions between metal ions and functional groups of microalgae, including amine (-NH 4 ), carboxyl (-COOH), phosphate (-PO 4 ), and hydroxyl (-OH) groups were evaluated. Nonetheless, this review also identifies the current challenges and future research directions for optimizing microalgae biotechnology in heavy metal bioremediation and nanoparticle biogenesis., (Copyright © 2024. Published by Elsevier B.V.)

Introduction Limited data have addressed frailty's role in cardiorenal risk among older adult patients with chronic kidney disease (CKD). We investigated whether frailty could predict major renal and cardiovascular events, healthcare utilization, and mortality in these patients. Methods We conducted a prospective cohort enrolling patients ≥ 75 years with a stable estimated glomerular filtration rate (eGFR) < 60 mL/min/1.73 m2. Frailty phenotype consists of shrinking, low activity, exhaustion, weakness, and slowness, scored 0 to 5. The primary composite renal outcome was a ≥ 25 % decrease in eGFR concurrent with CKD stage progression or dialysis initiation. Secondary outcomes included major adverse cardiovascular events (MACE), emergency room (ER) visits, all-cause mortality, and hospitalization. Using multivariate Cox models with/without competing risk analyses, we explored frailty's impact on these outcomes. Results Among 203 older CKD patients (mean age 81.6 ± 5.0 years, female 40.9 %, diabetes 33.0 %, body mass index 24.9 ± 3.7 kg/m2), 67.9% were frail. Over 3.47 years, 38.9% faced composite renal outcomes, 13.3% MACE, 15.3% mortality, and more than half utilized healthcare. Every one-point frailty elevated composite renal outcome risk by 28.0 % (HR: 1.28, 95% CI:1.03-1.59) and significantly increased secondary outcomes (hospitalization [HR: 1.24, 95% CI: 1.06-1.46], unexpected ER visit [HR: 1.20, [95% CI:1.03-1.39], and mortality [HR: 1.51, 95% CI: 1.06-2.16]) but not for MACE [HR: 1.43, 95% CI: 0.99-2.08]. Results were consistent across subgroups and competing risk analysis. Conclusion In CKD patients ≥ 75 years, frailty was associated with progressive kidney disease, increased mortality and healthcare utilization., (The Author(s). Published by S. Karger AG, Basel.)

Competing Interests: Conflicts of interest L.E.R. received clinical trial funding and grants from Mitsubishi Tanabe Pharma America Inc and Clinuvel Phar­maceuticals Ltd outside the submitted work. The other authors declare no conflicts of interest.

Climate change and other anthropogenic disturbances are increasing liana abundance and biomass in many tropical and subtropical forests. While the effects of living lianas on species diversity, ecosystem carbon, and nutrient dynamics are receiving increasing attention, the role of dead lianas in forest ecosystems has been little studied and is poorly understood. Trees and lianas coexist as the major woody components of forests worldwide, but they have very different ecological strategies, with lianas relying on trees for mechanical support. Consequently, trees and lianas have evolved highly divergent stem, leaf, and root traits. Here we show that this trait divergence is likely to persist after death, into the afterlives of these organs, leading to divergent effects on forest biogeochemistry. We introduce a conceptual framework combining horizontal, vertical, and time dimensions for the effects of liana proliferation and liana tissue decomposition on ecosystem carbon and nutrient cycling. We propose a series of empirical studies comparing traits between lianas and trees to answer questions concerning the influence of trait afterlives on the decomposability of liana and tree organs. Such studies will increase our understanding of the contribution of lianas to terrestrial biogeochemical cycling, and help predict the effects of their increasing abundance., (© 2024 John Wiley & Sons Ltd.)

The demand for astaxanthin has been increasing for many health applications ranging from pharmaceuticals, food, cosmetics, and aquaculture due to its bioactive properties. Haematococcus pluvialis is widely recognized as the microalgae species with the highest natural accumulation of astaxanthin, which has made it a valuable source for industrial production. Astaxanthin produced by other sources such as chemical synthesis or fermentation are often produced in the cis configuration, which has been shown to have lower bioactivity. Additionally, some sources of astaxanthin, such as shrimp, may denature or degrade when exposed to high temperatures, which can result in a loss of bioactivity. Producing natural astaxanthin through the cultivation of H. pluvialis is presently a demanding and time-consuming task, which incurs high expenses and restricts the cost-effective industrial production of this valuable substance. The production of astaxanthin occurs through two distinct pathways, namely the cytosolic mevalonate pathway and the chloroplast methylerythritol phosphate (MEP) pathway. The latest advancements in enhancing product quality and extracting techniques at a reasonable cost are emphasized in this review. The comparative of specific extraction processes of H. pluvialis biological astaxanthin production that may be applied to large-scale industries were assessed. The article covers a contemporary approach to optimizing microalgae culture for increased astaxanthin content, as well as obtaining preliminary data on the sustainability of astaxanthin production and astaxanthin marketing information., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

(1) Background: Understanding the relationship between community assembly and species coexistence is key to understanding ecosystem diversity. Despite the importance of wood-boring longhorn beetles (Cerambycidae) in forests, factors affecting their population dynamics, species richness, and ecological interactions remain underexplored. (2) Methods: We surveyed cerambycid beetles and plants within five plots each across three transects in tropical rainforests and temperate forests of Yunnan, China, known for its rich biodiversity and varied elevation gradients. We explored a range of analytical tools, including α -diversity comparisons, distance-decay relationships, redundancy analysis, β -dissimilarity metrics, and various neutral community model analyses. (3) Results: The results revealed a stark contrast between the two forest types: the tropical rainforests hosted 212 Cerambycidae and 135 tree species, whereas the temperate forests had only 16 Cerambycidae and 18 tree species. This disparity was attributed to differences in environmental heterogeneity and dispersal limitations. In temperate forests, pronounced environmental variability leads to steeper distance-decay relationships and reduced α-diversity of Cerambycidae, implying stronger dispersal constraints and weaker plant-beetle associations. Conversely, the more homogenous tropical rainforests exhibited stochastic processes that enhanced Cerambycidae diversity and plant-beetle interactions. (4) Conclusions: Our findings underscore that environmental heterogeneity, dispersal limitations, and host-specificity are pivotal in shaping biodiversity patterns in Cerambycidae, with significant variations across climatic zones.

Biofertilizers encompass microorganisms that can be applied to plants, subsequently establishing themselves within the plant's rhizosphere or internal structures. This colonization stimulates plant development by enhancing nutrient absorption from the host. While there is growing literature documenting the applications of microalgae-based and bacterial-based biofertilizers, the research focusing on the effectiveness of consortia formed by these microorganisms as short-term plant biofertilizers is notably insufficient. This study seeks to assess the effectiveness of microalgae-bacterial biofertilizers in promoting plant growth and their potential contribution to the circular economy. The review sheds light on the impact of microalgae-bacterial biofertilizers on plant growth parameters, delving into factors influencing their efficiency, microalgae-bacteria interactions, and effects on soil health. The insights from this review are poised to offer valuable guidance to stakeholders in agriculture, including farmers, environmental technologists, and businesses. These insights will aid in the development and investment in more efficient and sustainable methods for enhancing crop yields, aligning with the Sustainable Development Goals and principles of the circular economy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The low reactivity and volume expansion issue of steel slag limits its application as alternative to cement. Studies demonstrated that aqueous carbonation (AC) can enhance the cementitious properties of finely sized steel slag as a cementitious supplementary material (SCM). However, the impact of particle size on the CO 2 uptake capacity and its association of performance of carbonated steel slag remains unexplored. This study aims to optimize the grinding levels by examining the fineness of the steel slag used as SCM to reduce the high-energy consumption while maintaining the CO 2 sequestration and properties of SCM. The results show that reducing the size of steel slag is favorable for CO 2 sequestration (particle size 22.4-112.6 μm corresponds to sequestration of ∼88.5-37.9 kg CO 2 /t steel slag) and improve the leaching of Mg ions for mineralization. The life cycle assessment shows that the global warming potential of AC of steel slag is ∼96.2-24.9 kg CO 2 -eq/t steel slag, which can offset the carbon emissions due to further grinding. The 28-day compressive strength of the cement pastes blended with finer carbonated steel slag was also relatively higher due to the formation of mono-carboaluminates and stabilization of ettringite in facilitating the bond strength between the carbonated steel slag particle and the cement paste matrix. According to 3E (engineering, environmental and economic) triangle model, 22.4 μm steel slag powder showed the best comprehensive performance, including an increased revenue of 40.8 CNY/ton steel slag., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Surfactants have always been a prominent chemical that is useful in various sectors (e.g., cleaning agent production industry, textile industry and painting industry). This is due to the special ability of surfactants to reduce surface tension between two fluid surfaces (e.g., water and oil). However, the current society has long omitted the harmful effects of petroleum-based surfactants (e.g., health issues towards humans and reducing cleaning ability of water bodies) due to their usefulness in reducing surface tension. These harmful effects will significantly damage the environment and negatively affect human health. As such, there is an urgency to secure environmentally friendly alternatives such as glycolipids to reduce the effects of these synthetic surfactants. Glycolipids is a biomolecule that shares similar properties with surfactants that are naturally synthesized in the cell of living organisms, glycolipids are amphiphilic in nature and can form micelles when glycolipid molecules clump together, reducing surface tension between two surfaces as how a surfactant molecule is able to achieve. This review paper aims to provide a comprehensive study on the recent advances in bacteria cultivation for glycolipids production and current lab scale applications of glycolipids (e.g., medical and waste bioremediation). Studies have proven that glycolipids are effective anti-microbial agents, subsequently leading to an excellent anti-biofilm forming agent. Heavy metal and hydrocarbon contaminated soil can also be bioremediated via the use of glycolipids. The major hurdle in the commercialization of glycolipid production is that the cultivation stage and downstream extraction stage of the glycolipid production process induces a very high operating cost. This review provides several solutions to overcome this issue for glycolipid production for the commercialization of glycolipids (e.g., developing new cultivating and extraction techniques, using waste as cultivation medium for microbes and identifying new strains for glycolipid production). The contribution of this review aims to serve as a future guideline for researchers that are dealing with glycolipid biosurfactants by providing an in-depth review on the recent advances of glycolipid biosurfactants. By summarizing the points discussed as above, it is recommended that glycolipids can substitute synthetic surfactants as an environmentally friendly alternative., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)

Acetylation of glycerol to yield monoacetin (MAT), diacetin (DAT), and triacetin (TAT) over NiO-supported CeO 2 ( x NiO/CeO 2 ) catalysts is reported. The catalysts were synthesized utilizing a sol-gel technique, whereby different quantities of NiO ( x = 9, 27, and 45 wt%) were supported onto the CeO 2 substrate, and hexadecyltrimethylammonium bromide (CTABr) served as a porogen. The utilization of EDX elemental mapping analysis confirmed the existence of evenly distributed Ni 2+ ion and octahedral NiO nanoparticles on the CeO 2 surface through the DRS UV-Vis spectroscopy. The most active catalyst is 27NiO/CeO 2 based on TAT selectivity in the glycerol acetylation with ethanoic acid, attaining 97.6% glycerol conversion with 70.5% selectivity to TAT at 170 °C with a 1:10 glycerol/ethanoic acid molar ratio for 30 min using a non-microwave instant heating reactor. The 27NiO/CeO 2 is reusable without significant decline in catalytic performance after ten consecutive reaction cycles, indicating high structure stability with accessible active acidity.

A long-debated question in ecology is whether the hyper-diversity of tropical plant-feeding insects is a direct consequence of high tropical plant diversity or should be attributed to increases in host plant specialization. In this study, we used Cerambycidae (the wood-boring longhorn beetles whose larval stages feed on the xylems of trees and lianas) and plants as study materials to explore which hypothesis is more favoured. Multiple analyses were used to show the differences in host specificity of Cerambycidae in tropical and subtropical forests. From these analyses, we found that the alpha diversity of beetles in tropical forests was significantly higher than that in subtropical forests but not in plants. The relationship between plants and beetles was also closer in tropical areas than in subtropical areas. Our results imply that the wood-boring longhorn beetles show higher degrees of niche conservatism and host-specificity in tropical forests than in subtropical forests. The high diversity of wood-boring longhorn beetles in tropical forests might be explained to a large extent by their more finely partitioned diet breadth., (© 2023. The Author(s).)

Insulin resistance is a condition in which the target tissues have a decreased response to insulin signaling, resulting in glucose uptake defect, and an increased blood sugar level. Pancreatic beta cells thus enhance insulin production to compensate. This situation may cause further beta cell dysfunction and failure, which can lead diabetes mellitus (DM). Insulin resistance is thus an important cause of the development of type 2 DM. Insulin resistance has also been found to have a strong relationship with cardiovascular disease and is common in chronic kidney disease (CKD) patients. The mechanisms of insulin resistance in CKD are complex and multifactorial. They include physical inactivity, inflammation and oxidative stress, metabolic acidosis, vitamin D deficiency, adipose tissue dysfunction, uremic toxins, and renin-angiotensin-aldosterone system activation. Currently, available anti-diabetic agents, such as biguanides, sulfonylureas, thiazolidinediones, alfa-glucosidase inhibitors, glucagon-like peptide-1-based agents, and sodium-glucose co-transporter-2 inhibitors, have different effects on insulin resistance. In this short review, we describe the potential mechanisms of insulin resistance in CKD patients. We also review the interaction of currently available anti-diabetic medications with insulin resistance., Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article., (©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.)

Tropilaelaps mercedesae , one of the most devastating parasitic mites of honey bee Apis mellifera hosts, is a major threat to honey products by causing severe damage to honey bee colonies. Here, we recorded injury numbers caused by T. mercedesae to different body parts of the larval, pupal, and crippled adult stages of honey bee A . mellifera . We evaluated the relationship between infestation rate and injury numbers per bee for both larvae and pupae. We also noted the total bee numbers per beehive and examined the relationship between the infestation rate and population size. T . mercedesae infested all developmental stages of honey bees, with the highest injury numbers in the abdomens of bee pupae and the antennas of crippled adult bees. Although larvae received more injury numbers than pupae, both infestation rate and injury numbers decreased as the larval stage progressed to the pupal stage. The infestation rate increased as the population size per beehive decreased. This study provided new perspectives to the understanding of changes in the effects of T . mercedesae infestations on different developmental stages of honey bees. It also showed useful baseline information for screening honey bee stock that might have high defensive behaviors against mite infestation.

Stingless bees play a crucial role in the environment and agriculture as they are effective pollinators. Furthermore, they can produce various products that can be exploited economically, such as propolis and honey. Despite their economic value, the knowledge of microbial community of stingless bees, and their roles on the bees' health, especially in Thailand, are in its infancy. This study aimed to investigate the composition and the functions of bacterial community associated with Tetragonula pagdeni stingless bees using culture-independent and culture-dependent approaches with emphasis on lactic acid bacteria. The culture-independent results showed that the dominant bacterial phyla were Firmicutes, Proteobacteria and Actinobacteria. The most abundant families were Lactobacillaceae and Halomonadaceae. Functional prediction indicated that the prevalent functions of bacterial communities were chemoheterotrophy and fermentation. In addition, the bacterial community might be able to biosynthesize amino acid and antimicrobial compounds. Further isolation and characterization resulted in isolates that belonged to the dominant taxa of the community and possessed potentially beneficial metabolic activity. This suggested that they are parts of the nutrient acquisition and host defense bacterial functional groups in Thai commercial stingless bees., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Sinpoo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The ChAdOx1 nCoV-19 (AZD1222) vaccine is one of the most commonly delivered SARS-CoV-2 vaccines worldwide; however, few clinical studies have investigated its immunogenicity in dialysis patients. We prospectively enrolled 123 patients on maintenance hemodialysis at a medical center in Taiwan. All patients were infection-naive, had received two doses of the AZD1222 vaccine, and were monitored for 7 months. The primary outcomes were anti-SARS-CoV-2 receptor-binding domain (RBD) antibody concentrations before and after each dose and 5 months after the second dose and neutralization capacity against ancestral SARS-CoV-2, delta, and omicron variants. The anti-SARS-CoV-2 RBD antibody titers significantly increased with time following vaccination, with a peak at 1 month after the second dose (median titer, 498.8 U/mL; interquartile range, 162.5 to 1,050 U/mL), and a 4.7-fold decrease at 5 months. At 1 month after the second dose, 84.6, 83.7, and 1.6% of the participants had neutralizing antibodies against the ancestral virus, delta variant, and omicron variant, respectively, measured by a commercial surrogate neutralization assay. The geometric mean 50% pseudovirus neutralization titers for the ancestral virus, delta variant, and omicron variant were 639.1, 264.2, and 24.7, respectively. The anti-RBD antibody titers correlated well with neutralization capacity against the ancestral virus and delta variant. Transferrin saturation and C-reactive protein were associated with neutralization against the ancestral virus and delta variant. Although two doses of the AZD1222 vaccine initially elicited high anti-RBD antibody titers and neutralization against the ancestral virus and delta variant in hemodialysis patients, neutralizing antibodies against omicron variant were rarely detected, and the anti-RBD and neutralization antibodies waned over time. Additional/booster vaccinations are warranted in this population. IMPORTANCE Patients with kidney failure have worse immune response following vaccination compared to general population, but few clinical studies have investigated immunogenicity of ChAdOx1 nCoV-19 (AZD1222) vaccination in hemodialysis patients. Here, we showed two doses of AZD1222 vaccines lead to high seroconversion rate of anti-SARS-CoV-2 receptor-binding domain (RBD) antibodies, and more than 80% patients acquired neutralizing antibodies against ancestral virus and delta variant. However, seldom did they obtain neutralizing antibodies against the omicron variant. The geometric mean 50% pseudovirus neutralization titer against the ancestral virus was 25.9-fold higher than that against the omicron variant. Also, there was a substantial decay in anti-RBD titers with time. Our findings provided evidence supporting that more protective measures, including additional/booster vaccinations, is warranted in these patients during the current COVID-19 pandemic.

Widespread parasites, along with emerging threats, globalization, and climate change, have greatly affected honey bees' health, leading to colony losses worldwide. In this study, we investigated the detection of biotic stressors (i.e., viruses, microsporidian, bacteria, and fungi) in Apis cerana by surveying the colonies across different regions of Thailand (Chiang Mai in the north, Nong Khai and Khon Kaen in the northeast, and Chumphon and Surat Thani in the south, in addition to the Samui and Pha-ngan islands). In this study, we detected ABPV, BQCV, LSV, and Nosema ceranae in A. cerana samples through RT-PCR. ABPV was only detected from the samples of Chiang Mai, whereas we found BQCV only in those from Chumphon. LSV was detected only in the samples from the Samui and Pha-ngan islands, where historically no managed bees are known. Nosema ceranae was found in all of the regions except for Nong Khai and Khon Kaen in northeastern Thailand. Paenibacillus larvae and Ascosphaera apis were not detected in any of the A. cerana samples in this survey. The phylogenetic tree analysis of the pathogens provided insights into the pathogens' movements and their distribution ranges across different landscapes, indicating the flow of pathogens among the honey bees. Here, we describe the presence of emerging pathogens in the Asian honey bee as a valuable step in our understanding of these pathogens in terms of the decline in eastern honey bee populations.

Dialysis patients are at risk of both thromboembolic and bleeding events, while thromboembolism prevention and treatment may confer a risk of major bleeding. Gastrointestinal (GI) bleeding is a great concern which can result in high subsequent mortality rates. Our object was to clarify whether hemodialysis (HD) and peritoneal dialysis (PD) confer different incidence of GI bleeding, and further assist individualized decision-making on dialysis modalities. We conducted a population-based retrospective cohort study which included all incident dialysis patients above 18 years old derived from the National Health Insurance database from 1998 to 2013 in Taiwan. 6296 matched pairs of HD and PD patients were identified. A propensity score matching method was used to minimize the selection bias. The adjusted hazard ratio for GI bleeding was 1.13 times higher in the HD group than in the PD group, and data from the unmatched cohort and the stratified analysis led to similar results. Among subgroup analysis, we found that the use of anticoagulants will induce a much higher incidence of GI bleeding in HD patients as compared to in PD patients. We concluded that PD is associated with a lower GI bleeding risk than HD, and is especially preferred when anticoagulation is needed., (© 2023. The Author(s).)

With the rapid development of the economy and productivity, an increasing number of citizens are not only concerned about the nutritional value of algae as a potential new food resource but are also, in particular, paying more attention to the safety of its consumption. Many studies and reports pointed out that analyzing and solving seaweed food safety issues requires holistic and systematic consideration. The three main factors that have been found to affect the food safety of algal are physical, chemical, and microbiological hazards. At the same time, although food safety awareness among food producers and consumers has increased, foodborne diseases caused by algal food safety incidents occur frequently. It threatens the health and lives of consumers and may cause irreversible harm if treatment is not done promptly. A series of studies have also proved the idea that microbial contamination of algae is the main cause of this problem. Therefore, the rapid and efficient detection of toxic and pathogenic microbial contamination in algal products is an urgent issue that needs to be addressed. At the same time, two other factors, such as physical and chemical hazards, cannot be ignored. Nowadays, the detection techniques are mainly focused on three major hazards in traditional methods. However, especially for food microorganisms, the use of traditional microbiological control techniques is time-consuming and has limitations in terms of accuracy. In recent years, these two evaluations of microbial foodborne pathogens monitoring in the farm-to-table chain have shown more importance, especially during the COVID-19 pandemic. Meanwhile, there are also many new developments in the monitoring of heavy metals, algal toxins, and other pollutants. In the future, algal food safety risk assessment will not only focus on convenient, rapid, low-cost and high-accuracy detection but also be connected with some novel technologies, such as the Internet of Things (artificial intelligence, machine learning), biosensor, and molecular biology, to reach the purpose of simultaneous detection.

Evaluation of multiple barriers contributing to reproductive isolation between sympatric plant species is key to understanding the mechanism of their coexistence; however, such investigations in biodiversity hotspots are still rare. In this study, we investigated and compared geography, microhabitat, phenology, flora, and pollinators, in addition to pollen-pistil interactions, seed production, and seed germination of the closely related sympatric Salvia digitaloides and S. flava on Yulong Snow Mountain, Southwestern Yunnan, China. The geographic distribution of these species overlapped, but their adaptation to physical and chemical properties of soil microhabitats differed. They shared the same flowering time but differed in flower size, style length, nectar volume, sugar concentration, and flower longevity. Both species shared bumblebees as effective pollinators, but flower constancy for the two species was relatively strong. Pollen tube growth, seed production, and seed germination were lower in interspecific than in intraspecific crosses. Our study suggested that microhabitat and pollinator isolation acted as the most important isolating barriers in maintaining the coexistence of the two Salvia species. Our study also highlighted that post-pollination barriers play an important role in preventing the gene flow between these two Salvia species., Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

In recent years, researchers have proven that the employment of natural green components in the biogenesis of nanoparticles from microalgae species is one of the ways to delight the global environment issues. The application of nanotechnology with the exploitation of phycochemical produced from algae species is known as 'phyconanotechnology'. The use of biological compounds by microalgae as reducing agents for the synthesis of inorganic nanoparticles has shown promising results such as cost-effective and environmentally friendly. Different classifications of algae such as brown algae, red algae, green algae, and cyanobacteria are studied for the synthesis of different types of metal nanoparticles. It is also an important motive to acknowledge the mechanisms of the microalgae-mediated biosynthesis of nanoparticles via an intracellular pathway or extracellular pathway. Besides, microalgae species as biogenic sources preclude the use of conventional methods reagents, such as sodium borohydride (NaBH 4 ) and N,N-dimethylformamide (DMF), which further consolidates their position as the best choice for sustainable (economically and environmentally) nanoparticle synthesis compared to the conventional nanoparticles synthesis pathway., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Algal green energy has emerged as an alternative to conventional energy production using fossil fuels. Microbial fuel cells (MFCs), photosynthetic microbial fuel cells (PMFCs) and biophotovoltaic (BPV) platforms have been developed to utilize microalgae for bioelectricity generation, wastewater treatment and biomass production. There remains a lack of research on marine microalgae in these systems, so to the best of our knowledge, all information on their integration in these systems have been gathered in this review, and are used to compare with the interesting studies on freshwater microalgae. The performance of the systems is extremely reliant on the microalgae species and/or microbial community used, the size of the bio-electrochemical cell, and electrode material and distance used. The mean was calculated for each system, PMFC has the highest average maximum power density of 344 mW/m 2 , followed by MFC (179 mW/m 2 ) and BPV (58.9 mW/m 2 ). In addition, the advantages and disadvantages of each system are highlighted. Although all three systems face the issue of low power outputs, the integration of a suitable energy harvester could potentially increase power efficiency and make them applicable for lower power applications., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication., (© King Abdulaziz City for Science and Technology 2022.)

Background: Cancer disease is a growing health problem in developing and developed countries. Hypoxia-inducible factor-1a (HIF1α) is a transcription factor responsible for expressing several proteins involved in angiogenesis. Quercetin can suppress HIF1α expression due to the inhibition of protein synthesis. However, to date, the study exploring the potential of quercetin in repressing HIF1α through its degradation mechanism has never been done. An in silico study is needed as a preliminary study to understand the mechanism underlining this possibility., Objective: This study aimed to investigate the potential of quercetin in regulating HIF1α expression through the ubiquitin degradation pathway by in silico study., Methods: This study was performed by in silico analysis, including biological activity prediction, 3D protein structure collection, protein-ligand and protein-protein docking, and the visualization of the docking results., Results: The probability activity (Pa) score of quercetin as an HIF1α expression inhibitor was 0.969. In the absence of quercetin, the center-weighted score of HIF1α - pVHL, HIF1α - FIH, and HIF1α - PHD2 was -699.4 kJ/mol, -846.0 kJ/mol, and -650.5 kJ/mol, respectively. In the presence of quercetin, the weighted score of HIF1α - pVHL, HIF1α - FIH, and HIF1α - PHD2 was reduced to -728.1 kJ/mol, -854.2 kJ/mol, and -650.5 kJ/mol, respectively., Conclusion: Quercetin could directly promote HIF1α and pVHL interaction, thus increasing the degradation of HIF1α by ubiquitin-dependent pathway., Competing Interests: There are no conflicts of interest., (© 2022 Sri Puji Astuti Wahyuningsih, Amy Saik Yi Hsan, Looi Mee Lee, Vuanghao Lim, Lionel In Lian Aun, Sephia Tiara Marviella, Firli Rahmah Primula Dewi.)

At temperatures below the critical temperature, discontinuities in the isotherms are one critical issue in the design and construction of separation units, affecting the level of confidence for a prediction of vapor-liquid equilibriums and phase transitions. In this work, we study the molecular mechanisms of fluids that involve the vapor-liquid phase transition in bulk and confinement, utilizing grand canonical (GCE) and meso-canonical (MCE) ensembles of the Monte Carlo simulation. Different geometries of the mesopores, including slit, cylindrical, and spherical, were studied. During phase transitions, condensation/evaporation hysteretic isotherms can be detected by GCE simulation, whereas employing MCE simulation allows us to investigate van der Waals (vdW) loop with a vapor spinodal point, intermediate states, and a liquid spinodal point in the isotherms. Depending on the system, the size of the simulation box, and the MCE method, we are able to identify three distinct groups of vdW-type isotherms for the first time: (1) a smooth S-shaped loop, (2) a stepwise S-shaped loop, and (3) a stepwise S-shaped loop with just a vertical segment. The first isotherm type is noticed in the bulk and pores having small box sizes, in which vapor and liquid phases are close and not clearly identified. The second and the third types occurred in the bulk, cylindrical, and slit mesopores with sufficiently large spaces, where vapor and liquid phases are distinctly separated. Results from our studies provide an insight analysis into vapor-liquid phase transitions, elucidating the effect of the confinement of fluid behaviors in a visual manner.

In recent decades, the use of controlled low-strength material (CLSM) in densely populated cities has increased. CLSM is designed for future excavation with great fluidity, appropriate early strength, and low final strength. CLSM mixtures exhibit variable strength properties and performance due to the distinctive features of wastes (i.e., combustion residues, industry slags, and construction and other solid wastes) produced from various sources. CLSM should increase early strength quickly enough to allow traffic to resume within a few hours while maintaining a low strength for future re-excavation. It is suggested that the initial mixture design for each waste reported in the literature be changed until the combination meets the application standards defined in ACI 229R-13. The effects of adjusting other ingredients (i.e., cement, water, and admixtures) in the wastes incorporated into CLSM mixtures on the strength and re-excavatability properties are also detailed and discussed in this review. From practical and economic perspectives, the supply of materials in the waste streams, transport distance, and material properties and cost are important aspects to consider before their introduction to the construction industry., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Microalgae is an autotrophic organism with fast growth, short reproduction cycle, and strong environmental adaptability. In recent years, microalgae and the bioactive ingredients extracted from microalgae are regarded as potential substitutes for raw materials in the pharmaceutical and the cosmetics industry. In this review, the characteristics and efficacy of the high-value components of microalgae are discussed in detail, along with the sources and extraction technologies of algae used to obtain high-value ingredients are reviewed. Moreover, the latest trends in biotherapy based on high-value algae extracts as materials are discussed. The excellent antioxidant properties of microalgae derivatives are regarded as an attractive replacement for safe and environmentally friendly cosmetics formulation and production. Through further studies, the mechanism of microalgae bioactive compounds can be understood better and reasonable clinical trials conducted can safely conclude the compliance of microalgae-derived drugs or cosmetics to be necessary standards to be marketed., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In this study, municipal solid waste incineration fly ash (MSWIFA) was pretreated with CO 2 via slurry carbonation (SC) and dry carbonation coupled with subsequent water washing (DCW). Both the treated MSWIFAs were then used as cement replacement in cement pastes by weight of 10%, 20% and 30% to investigate the influence on hydration mechanisms, physico-mechanical characteristics and leaching properties. The results showed that carbonates formed on the surface of SC-MSWIFA particles were finer (primarily 20-50 nm calcite) than those from the corresponding DCW-MSWIFA (mostly 130-200 nm vaterite). Hence, SC-MSWIFA blended cement pastes led to shorter setting time and higher early compressive strength than the DCW-MSWIFA pastes. In contrast, the presence of vaterite-rich DCW-MSWIFA in the blended cement pastes could accelerate the cement hydration after 24 h. Both the CO 2 -pretreated MSWIFA can replace cement up to 30% without sacrificing the long-term strength and mechanical properties of cement pastes, demonstrating excellent performance as a supplementary cementitious material. Moreover, volume stability in terms of expansion and lead leaching of CO 2 -pretreated MSWIFA cement pastes were far below the regulatory limits., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The rapid increase in steel slag generation globally highlights the urgent need to manage the disposal or utilization processes. In addition to conventional landfill disposal, researchers have successfully reused steel slag in the construction, chemical, and agricultural fields. With the large portions of alkaline silicate mineral content, steel slag can also be used as a suitable material for carbon capture to mitigate global warming. This article comprehensively reviews the environmental performance of steel slag utilization, especially emphasizing quantitative evaluation using life cycle assessment. This paper first illustrates the production processes, properties, and applications of steel slag, and then summarizes the key findings of the environmental benefits for steel slag utilization using life cycle assessment from the reviewed literature. This paper also identifies the limitations of quantifying the environmental benefits using life cycle assessment. The results indicate steel slag is largely utilized in pavement concrete and/or block as a substitution for natural aggregates. The associated environmental benefits are mostly attributed to the avoidance of the large amount of cement utilized. The environmental benefits for the substitution of traditional energy-intensive material and carbonation treatment are further discussed in detail. Due to the presence of heavy metals, the potential risks to human and ecological health caused by the manufacturing process and usage stage are examined. Finally, the current challenges and global social implications for steel slag valorization are summarized., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The litter of persistent organic pollutants (POPs) into the water streams and soil bodies via industrial effluents led to several adverse effects on the environment, health, and ecosystem. For the past decades, scientists have been paying efforts in the innovation and development of POPs removal from wastewater treatment. However, the conventional methods used for the removal of POPs from wastewater are costly and could lead to secondary pollution including soil and water bodies pollution. In recent, the utilization of green mechanisms such as biosorption, bioaccumulation and biodegradation has drawn attention and prelude the potential of green technology globally. Microalgae-bacteria consortia have emerged to be one of the latent wastewater treatment systems. The synergistic interactions between microalgae and bacteria could proficiently enhance the existing biological wastewater treatment system. This paper will critically review the comparison of conventional and recent advanced wastewater treatment systems and the mechanisms of the microalgae-bacteria symbiosis system., (Copyright © 2021 Elsevier Ltd. All rights reserved.)