Search

Selective growth of Pd and Pt on the edges of Au(111) supported FeO islands was observed. The complicated interfaces formed in selective growth raise a platform for the research of edge involved catalytic reactions.

Agriculture is a significant contributor to global greenhouse gas emissions, and reducing carbon emissions in this sector is essential for mitigating global warming. To achieve China’s targets of carbon peak by 2030 and carbon neutrality by 2060, promoting low-carbon agricultural technology (LCAT) is fundamental. This study examines the impact of farmers’ risk perception on LCAT adoption behavior and its productivity effects with the Ordered Probit regression method, using micro survey data from 531 farmers in Shaanxi Province, China. The results show that farmers with stronger risk perceptions were more likely to adopt LCAT, based on their loss aversion characteristics. Additionally, farmers’ perceptions of yield, market, and climate risks positively influence the adoption of LCAT, with market risk perception having the strongest effect. Adopting LCAT has significant production and spillover effects, improving the output rate of farmers’ operating farmland and neighboring plots by 2.4% and 1.2%, respectively, for each additional measure adopted. This study contributes to the perception and loss aversion literature by examining farmers’ adoption of low-carbon agricultural practices. This study sheds light on the importance of risk perception in the adoption of sustainable agricultural practices and can inform policies aimed at promoting the adoption of LCAT for achieving sustainable agriculture and mitigating climate change, highlighting the crucial role of sustainable environmental management in the agricultural sector.

Chemical reactions such as bond dissociation and formation assisted by localized surface plasmons (LSPs) of noble metal nanostructures hold promise in solar-to-chemical energy conversion. However, the precise control of localized plasmons to activate a specific moiety of a molecule, in the presence of multiple chemically equivalent parts within a single molecule, is scarce due to the relatively large lateral distribution of the plasmonic field. Herein, we report the plasmon-assisted dissociation of a specific molecular site (C-Si bond) within a polyfunctional molecule adsorbed on a Cu(100) surface in the scanning tunneling microscope (STM) junction. The molecular site to be activated can be selected by carefully positioning the tip and bringing the tip extremely close to the molecule (atomistic approach), thereby achieving plasmonic nanoconfinement at the tip apex. Furthermore, multiple reactive sites are activated in a sequential manner at the sub-molecular scale, and different sets of products are created and visualized by STM topography and density functional theory (DFT) modeling. The illustration of site-selective activation achieved by localized surface plasmons implies the realization of molecular-scale resolution for bond-selected plasmon-induced chemistry.

Gaining valuable insight into chemistry-related fields, such as molecular and catalytic systems, surface science, and biochemistry, requires probing physical and chemical processes at the sub-nanoscale level. Recent progress and advancements in nano-optics and nano-photonics, particularly in scanning near-field optical microscopy, have enabled the coupling of light with nano-objects using surface plasmons with sub-nanoscale precision, providing access to photophysical and photochemical processes. Herein, this review highlights the basic concepts of surface plasmons and recent experimental findings of tip-assisted plasmon-induced research works and offers a glimpse into future perspectives.

The present study aims to investigate the influences of potassium (K) supply and harvest time on selected growth, physiology and quality factors of Ephedra sinica Stapf (ESS). A field plot experiment was conducted under 10 levels of K supply (K1–K10, 0–538.3 kg ha−1). ESS was sampled randomly with uniform distribution locations of each plot on August 27 (H1), September 15 (H2), October 6 (H3), and October 27 (H4) in 2018. The late-harvested ESS had low SPAD value, peroxidase activity, cell membrane permeability, and high 10 cm stem weight, phenylalanine ammonia-lyase activity, free proline, ephedrine, total alkaloids, or flavonoids contents. The fertilizer dose of 59.8–239.3 kg ha−1 (K2-K5) increased the 10 cm stem weight, stem or root fresh and dry weights, the SPAD value, free proline content, peroxidase activity, reduced the root activity. The ephedrine, pseudoephedrine, total alkaloids, and flavonoids contents increased with the increase of K supply before K7 (358.9 kg ha−1). The harvest time influenced the increase of these quality factors resulting from one unit of K application. The harvested E. sinica in H3 increased the ephedrine content 85.97%, pseudoephedrine 21.53%, total alkaloids 38.02%, and total flavonoids 66.43%, compared with their average values of H1. The dose of 239.3 kg ha−1 (K5) reduced the ephedrine content 14.99%, pseudoephedrine 8.07%, total alkaloids 16.4%, and total flavonoids 28.71%, compared with K7, however increased the stem fresh and dry weight 163.21% and 125.70%, respectively. The late-harvested ESS had high contents of quality factors and good quality. The K dose of 239.3 kg ha−1 was appropriate for cultivated ESS high yield and quality accumulation.

An outbreak of COVID-19, caused by infection with SARS-CoV-2 in Wuhan, China in December 2019, spread throughout the country and around the world, quickly. The primary detection technique for SARS-CoV-2, the reverse-transcription polymerase chain reaction (RT-PCR)–based approach, requires expensive reagents and equipment and skilled personnel. In addition, for SARS-CoV-2 detection, specimens are usually shipped to a designated laboratory for testing, which may extend the diagnosis and treatment time of patients with COVID-19. The latest research shows that clustered regularly interspaced short palindromic repeats (CRISPR)–based approaches can quickly provide visual, rapid, ultrasensitive, and specific detection of SARS-CoV-2 at isothermal conditions. Therefore, CRISPR-based approaches are expected to be developed as attractive alternatives to conventional RT-PCR methods for the efficient and accurate detection of SARS-CoV-2. Recent advances in the field of CRISPR-based biosensing technologies for SARS-CoV-2 detection and insights into their potential use in many applications are reviewed in this article.

With the unique properties, layered transition metal dichalcogenide (TMD) and its heterostructures exhibit great potential for applications in electronics. The electrical performance, e.g., contact barrier and resistance to electrodes, of TMD heterostructure devices can be significantly tailored by employing the functional layers, called interlayer engineering. At the interface between different TMD layers, the dangling-bond states normally exist and act as traps against charge carrier flow. In this study, we propose a technique to suppress such carrier trap that uses enhanced interlayer hybridization to saturate dangling-bond states, as demonstrated in a strongly interlayer-coupled monolayer-bilayer PtSe2 heterostructure. The hybridization between the unsaturated states and the interlayer electronic states of PtSe2 significantly reduces the depth of carrier traps at the interface, as corroborated by our scanning tunnelling spectroscopic measurements and density functional theory calculations. The suppressed interfacial trap demonstrates that interlayer saturation may offer an efficient way to relay the charge flow at the interface of TMD heterostructures. Thus, this technique provides an effective way for optimizing the interface contact, the crucial issue exists in two-dimensional electronic community.

Background: Glutaric acidemia type 1 (GA1) is a rare organic acid metabolism disorder resulted from the deficiency of glutaryl-CoA dehydrogenase (GCDH). There are few studies on GA1 in China. This article is designed to investigate the clinical, biochemical and molecular characterizations of patients with GA1 in Henan province, in order to get further understanding of this disease .Results: The incidence of neonatal GA1 in Henan population was 1/172483. All patients had increased glutarylcarnitine (C5DC), glutaric acid (GA) and 3-hydroxyglutaric acid (3-OH-GA) levels. Twenty mutations were discovered in 14 patients. Four of them were novel: c.523G>A (p.G175S), c.1297G>C (p.A433P), c.467G>A (p.G156D) and c.1125T>G (p.C375W). The most common mutation was c.1064G>A (p.R355H), of which the allelic frequency was 17.86% (5/28), following by c.1109T>C (p.L370P), c.648G>A (p.S216S), c.395G>A (p.R132Q) and c.938G>A (p.R313Q), which had the same allelic frequency 7.14% (2/28). 84.6%(11/13) patients displayed abnormal brain MRI findings, of which cerebral dysplasia and arachnid/ependyma cysts(38.5%,5/13) were most commonly. Clinical features varied widely from asymptomatic to severe encephalopathy. Patients diagnosed by newborn screening had better outcome. A strong correlation was discovered among increasing disability in motor, speech and cognitive function. There were statistical significance differences in motor function(p=0.024) and speech function (p=0.008), but no statistical significance difference in cognitive function (p=0.106) between NBS group and non-NBS group.Conclusions: Our study provided comprehensive information about GA1 in Henan population, including morbidity, clinical, biochemical, molecular and outcome features. The novel variants expanded the GCDH variant spectra. This work emphasize that professional management of NBS programs and experienced genetic metabolic disease experts will be very important and urgent.

Using gauge data during 1979–2013, the spatial and temporal characteristics of winter clear-sky days (CSDs) in China are investigated. The frequency of winter CSDs in China has two independent and significant modes, which account for 41% and 10% of the total variance, respectively. Based on evidence from observational analysis and numerical experiments, the formation mechanisms of these two leading modes are unraveled. The first mode is a homogenous mode with coherent year-to-year variation of CSDs over most parts of China, and is closely related to a large-scale cyclonic/anticyclonic anomaly over the Asian continent. Anomalous Indian Ocean SST and Arctic sea ice are responsible for the associated large-scale circulation anomalies via inducing a mid-to-high latitude Rossby wave train. The Indian Ocean SST warming can induce wave energy propagation upwards to the stratosphere and then northwards to the polar region, where it moves down to the troposphere and then travels southeastwards from the polar region to East Asia. Meanwhile, the decreased Arctic sea ice can directly induce a Rossby wave train emanating from the polar region that travels southeastwards into East Asia. These two factors jointly lead to a dominant equivalent barotropic cyclonic/anticyclonic anomaly over China, and in turn the homogenous pattern of winter CSDs in the region. The second mode is a seesaw mode with out-of-phase variations of CSDs between northern and southern China. This mode is closely related to a meridional dipole circulation anomaly pattern over the East Asian sector, which is generated by the so-called ‘Mega-ENSO’ SST anomaly over the Pacific Ocean. This Mega-ENSO SSTA pattern can induce convective heating/cooling over the tropical western Pacific, which further leads to the meridional dipole circulation anomaly pattern over the East Asian sector, resulting in the south-to-north seesaw mode of winter CSDs in China.

Mineral nutrients play important roles in the growth and metabolism of Ephedra intermedia, and are affected by soil factors. Fifteen elements were measured from wild E. intermedia as well as their growing soils using inductively coupled plasma mass spectroscopy to investigate the influences and characteristics of herb elements. The pH, cation exchange capacity, humus and soil mechanical composition were also determined in rhizosphere soils. Results showed that E. intermedia stems contained high N, low P concentrations in macronutrients and high Fe in micronutrients, and enriched N, S, Cl, P and Sr from soils. The 15 herb elements were affected by one or more soil factors, and K, P, Zn, Fe and Mn were important soil elements that influenced the mineral accumulation of E. intermedia. This study was useful for the artificial cultivation of wild E. intermedia.

In this research, the effects of natural seawater (SW) on the properties of alkali-activated slag/fly ash (AASF) are studied. AASF prepared with deionized water is set as the reference mixture. The results showed that the use of natural SW resulted in a prolonged setting time and lower heat release, but no obvious impact on the flowability of AASF specimens. The long-term compressive strength became higher when SW was used, whereas the corresponding flexural strength and fractural toughness turned lower. The use of SW induced the formation of new products that were not identified in the reference mixture, such as Cl–hydrocalumite and gypsum. In addition, it is evidenced that the dissolution of fly ash (FA) particles was significantly delayed with the incorporation of SW. All these results were related to the various ions introduced by the natural SW and their interactions with the alkaline activator as well as the precipitation of salts on slag and FA surfaces or in the matrix.

In this paper, the Richtmyer–Meshkov instability of a flat gas interface driven by perturbed and reflected shock waves is numerically investigated. The flat gas interface evolves into a “Λ”-shaped structure with a central N2 cavity and steps on both sides, due to the impaction of the perturbed shock wave. After the secondary collision of the reflected shock wave from the high-density region to the low-density region, the gas interface first undergoes phase inversion, and the “Λ” interface then evolves into a bubble and spike structure. Three cases of different Atwood numbers, N2/SF6, N2/Kr, and N2/CO2, are studied. The collision time and position of the reflected shock wave and the interface, the induced spikes, bubbles and gas mixing, are compared in detail. The formation of the spike and bubble is related to the RM instability developed by the collision of the reflected shock wave and the perturbed interface, in which the effect of baroclinic vorticity is highlighted. With the increase in the Atwood number, the density gradient and the baroclinic vorticity become larger, which induces more vortex along the interface. Kelvin Helmholtz unstable vortices are generated on the “legs” of the spikes due to shearing. The main spike structure is stretched and broken with the effect of the vortex, forming a turbulent mixing zone.

Advanced wastewater treatment plants are widely used in most large-scale pig farms in southern China. However, the fate of antibiotic resistance genes (ARGs) and their association with bacterial and archaeal communities during advanced wastewater treatment remain unclear. In this study, the profiles of ARGs in typical advanced wastewater treatment plants were surveyed using metagenomic analysis. The results showed that 279- 326 different subtypes of ARGs were detected in raw wastewater, with a total abundance of 5.98 ± 0.48 copies per bacterial cell. During the advanced wastewater treatment, the abundance and number of ARGs were significantly reduced. Microbial communities (bacteria and archaea) contributed the most to the variation in ARG abundance and composition (PCA axis_1), accounting for 10.8 % and 15.7 %, respectively, followed by mobile genetic elements (MGEs) and physicochemical factors. Special attention should be given to potential pathogenic bacteria such as Escherichia, Streptococcus, Enterococcus and Staphylococcus and archaea such as Methanocorpusculum, Candidatus Methanoplasma and Candidatus Methanomethylophilus, which were important potential ARG hosts. Bacterial communities may indirectly affect ARG variation by affecting archaeal communities. These findings indicated that ARG levels in pig farm wastewater can be effectively reduced during advanced treatment and highlighted the important role played by archaea, which should not be ignored.

This paper presents numerical modeling of nanoparticles injected into cementitious material using the electromigration method. In order to remediate leaking wells in CO2 storage formations,...

Two-dimensional boron monolayers (i.e., borophene) hold promise for a variety of energy, catalytic, and nanoelectronic device technologies due to the unique nature of boron-boron bonds. To realize its full potential, borophene needs to be seamlessly interfaced with other materials, thus motivating the atomic-scale characterization of borophene-based heterostructures. Here, we report the vertical integration of borophene with tetraphenyldibenzoperiflanthene (DBP) and measure the angstrom-scale interfacial interactions with ultrahigh-vacuum tip-enhanced Raman spectroscopy (UHV-TERS). In addition to identifying the vibrational signatures of adsorbed DBP, TERS reveals subtle ripples and compressive strains of the borophene lattice underneath the molecular layer. The induced interfacial strain is demonstrated to extend in borophene by ∼1 nm beyond the molecular region by virtue of 5 A chemical spatial resolution. Molecular manipulation experiments prove the molecular origins of interfacial strain in addition to allowing atomic control of local strain with magnitudes as small as ∼0.6%. In addition to being the first realization of an organic/borophene vertical heterostructure, this study demonstrates that UHV-TERS is a powerful analytical tool to spectroscopically investigate buried and highly localized interfacial characteristics at the atomic scale, which can be applied to additional classes of heterostructured materials.

Flexible polycyclic aromatic hydrocarbons (PAHs) have experienced a surge of research with the discovery of nanographenes that can be fabricated on surfaces. The flexibility in σ bonds within aroma...

The quality of medicinal plants is closely related to the ecological factors in growing locations, and the curative ingredients and biopotency reflect their quality. This study determined the active ingredient concentrations (tenuifolin, polygalaxanthone III, 3,6′-disinapoyl sucrose, sibiricose A5, sibiricose A6 and sibiricaxanthone B) and biopotency (intelligence, tranquilisation and expectorant) of Polygala tenuifolia Willd. from the main cultivation regions of China. Moreover, this study discussed how 36 ecological factors (e.g. climate and soil condition) influenced these ingredients and their biopotency. The relationship between ecological factors and quality factors was analyzed step by step using correlation analysis, principal component analysis and path analysis. Nine ecological factors: annual mean temperature, annual sunshine duration, soil pH, Cl, Sr, Ca, S, B and exchangeable K concentration were considered as key factors that influenced the quality of cultivated P. tenuifolia. This study provided a novel approach for the quality evaluation of P. tenuifolia and a rational pattern for exploring the complex relationship between ecological factors and P. tenuifolia quality.

The objective of the work is to understand the change in toughness caused by the introduction of repair materials into the hardened cement paste sheath surrounding a well bore. If the impact on toughness of repair material introduced into the hardened cement sheath is not considered, there exists the possibility that a leaking well may hold pressure after repair, but over the long-term crack development is encouraged. To address this, this paper presents a theoretical study of the effect of introducing nanoparticles on the density and fracture toughness of aged class G oil cement paste. The introduction of repair material in cement paste is studied in the framework of fracture mechanics which is employed to determine the effect of the cement paste microstructure near an existing crack tip. The impact of surrounding cracks, pores, and stiff nanoparticles on the fracture toughness of the material is formulated analytically using an extension to the classical J -integral. There is a linear relationship between the J -integral value and toughness if we assume a crack will propagate linearly from the defect it was initiated from. Such a formulation allows us to perform a forward analysis in the design, avoiding complex numerical models during the nanoparticle selection process in the repair. We find that the introduction of nanoparticles into aged cementitious materials increases its density which, in turn, leads to an increase in fracture toughness. The crack-tip stress concentrations near the stiff nanoparticles encourage further brittle cracking of the material, decreasing toughness. Thus, the resulting fracture toughness of the matrix is a trade-off between increasing density and stress concentration. The mechanical behavior of the cement paste containing nanoparticles was also characterized experimentally by standard three-point bending tests. Nanoparticles are injected in hardened cement paste cylinders by an electrokinetic method and samples are cut to conduct standardized toughness tests. Both the experimental and analytical results agree that an optimum particle content can be found which extends the lifetime of the cement paste without encouraging further crack development in the material.

On-surface synthesis via covalent coupling of adsorbed precursor molecules on metal surfaces has emerged as a promising strategy for the design and fabrication of novel organic nanoarchitectures with unique properties and potential applications in nanoelectronics, optoelectronics, spintronics, catalysis, etc. Surface-chemistry-driven molecular engineering (i.e., bond cleavage, linkage, and rearrangement) by means of thermal activation, light irradiation, and tip manipulation plays critical roles in various on-surface synthetic processes, as exemplified by the work from the Ernst group in a prior issue of ACS Nano. In this Perspective, we highlight recent advances in and discuss the outlook for on-surface syntheses and molecular engineering of carbon-based nanoarchitectures.

Transition metal oxide layers on metal surfaces often show superior catalytic performance as compared to conventional, i.e., metal-on-oxide, systems. In this work, we studied the CO oxidation reaction over FeO supported by Pt(1 1 1) and observed strong size effects on the reactivity. The monolayer islands below 5 nm in size showed a light-off temperature for CO2 production 200 K lower than for the 30 nm islands. In an attempt to rationalize the size effect, we have performed oxygen desorption experiments and have combined them with an extended DFT analysis to provide insight into the bonding of various oxygen species in such systems. The theoretical results on isolated FeO2 islands indicate a substantially lower stability of the boundary oxygen atoms as compared with the same islands embedded into an FeO layer in qualitative agreement with experiment showing that the smaller the island, the lower the desorption temperature. To the best of our knowledge, the results demonstrate the first example of a size effect in oxidation catalysis on two-dimensional systems.

High-quality economic development is an important approach for achieving sustainable economic development, and it is an essential condition for coordinated development between economic systems and ecosystems. This paper starts from five key points, namely, “innovation, coordination, opening-up, sharing and greenness”, to construct an evaluation system for the index of high-quality economic development, using the AHP and EVM methods to measure the level of high-quality economic development of 30 regions in China from 2004 to 2019. It uses the kernel density estimation model (hereinafter referred to briefly as KDE) and clustering method to analyze time evolution trends and spatial variation characteristics. Moreover, the LSE model is adopted to explore and analyze the factors influencing high-quality economic development in different regions. Additionally, the driving forces of China’s high-quality economic development are analyzed by means of path analysis combined with the average value of each index. The results show the following: (1) The high-quality economic development of 30 regions in China (excluding Hong Kong, Macao, Taiwan and Tibet) is spatially clustered, with obviously different development levels, characterized by the eastern region being better developed than the central and western regions. (2) With the passage of time, the polarization of China’s 30 regions has been alleviated, but they are still facing challenging development situations; (3) The factors affecting the high-quality economic development of these 30 regions in China can be divided into four types: three-factors, four-factors-I, four-factors-II and five-factors. Contributing regional factors show different distribution characteristics. The above conclusion provides a reference and scientific basis for the government to formulate policies of high-quality economic development and to solve problems facing coordinated sustainable development among regional societies, their economies and the environment.

Surface-bound reactions have become a viable method to develop nanoarchitectures through bottom-up assembly with near atomic precision. However, the bottom-up fabrication of nanostructures on surfaces requires careful consideration of the intrinsic properties of the precursors and substrate as well as the complex interplay of any interactions that arise in the heterogeneous two-dimensional (2D) system. Therefore, it becomes necessary to consider these systems with characterization methods sensitive to such properties with suitable spatial resolution. Here, low temperature ultrahigh vacuum scanning tunneling microscopy (STM) and tip-enhanced Raman spectroscopy (TERS) were used to investigate the formation of 2D covalent networks via coupling reactions of tetra(4-bromophenyl)porphyrin (Br4TPP) molecules on a Ag(100) substrate. Through the combination of STM topographic imaging and TERS vibrational fingerprints, the conformation of molecular precursors on the substrate was understood. Following the thermally activated coupling reaction, STM and TERS imaging confirm the covalent nature of the 2D networks and suggest that the apparent disorder arises from molecular flexibility.

Scanning probes measure how nearby oxygen molecules affect triplet lifetimes of pentacene

Autism spectrum disorder (ASD) is a heterogeneous group of neurodevelopmental disorders characterized by deficits in communication, impaired social interaction, and repetitive or restricted interests and behaviors. We have recently shown that neuronal nitric oxide synthase (nNOS) expression was reduced in the basolateral amygdala of mice after postnatal valproic acid exposure. However, the specific role of nNOS downregulation in mice remains to be elucidated. Herein, we investigated the behavioral alternations of naive mice with a recombinant adeno-associated virus (rAAV)-mediated knockdown of nNOS in a comprehensive test battery, including the social interaction, marble burying, self-grooming, and open field tests. Further, the electrophysiological and surface expression changes induced by nNOS deficiency of the basolateral amygdala in these animals were examined. Our results show that nNOS knockdown displayed typical symptoms of ASD-like behaviors, such as reduced social interaction and communication, elevated stereotypes, and anxiety in mice. Surprisingly, we found that nNOS knockdown exhibited greatly reduced excitatory synaptic transmission concomitant with the lower surface expression of GluN2B-containing N-methyl-D-aspartate receptors and postsynaptic density protein 95 in mice. These findings support a notion that dysregulation of nNOS might contribute to ASD-associated phenotypes, with disease pathogenesis most likely resulting from deficits in excitatory synaptic transmission.

Tip-enhanced Raman spectroscopy (TERS), a cutting-edge near-field spectroscopic tool, provides invaluable chemical insight with impressive spatial resolution in chemistry-related fields such as molecular and catalytic systems, surface science, two-dimensional materials, and biochemistry. High-resolution TERS, in particular, which has advanced exceptionally in the last five years, provides a unique opportunity to scrutinize single molecules individually. Here, this perspective places emphasis on the basic concepts and recent experimental findings of this state-of-the-art research and concludes with a glimpse of future prospects.

Fundamental understanding of chemistry and physical properties at the nanoscale enables the rational design of interface-based systems. Surface interactions underlie numerous technologies ranging from catalysis to organic thin films to biological systems. Since surface environments are especially prone to heterogeneity, it becomes crucial to characterize these systems with spatial resolution sufficient to localize individual active sites or defects. Spectroscopy presents as a powerful means to understand these interactions, but typical light-based techniques lack sufficient spatial resolution. This review describes the growing number of applications for the nanoscale spectroscopic technique, tip-enhanced Raman spectroscopy (TERS), with a focus on developments in areas that involve measurements in new environmental conditions, such as liquid, electrochemical, and ultrahigh vacuum. The expansion into unique environments enables the ability to spectroscopically define chemistry at the spatial limit. Through the confinement and enhancement of light at the apex of a plasmonic scanning probe microscopy tip, TERS is able to yield vibrational fingerprint information of molecules and materials with nanoscale resolution, providing insight into highly localized chemical effects.

The reversible control of a single spin of an atom or a molecule is of great interest in Kondo physics and a potential application in spin based electronics.Here we demonstrate that the Kondo resonance of manganese phthalocyanine molecules on an Au(111) substrate have been reversibly switched off and on via a robust route through attachment and detachment of single hydrogen atom to the magnetic core of the molecule. As further revealed by density functional theory calculations, even though the total number of electrons of the Mn ion remains almost the same in the process, gaining one single hydrogen atom leads to redistribution of charges within 3d orbitals with a reduction of the molecular spin state from S = 3/2 to S = 1 that directly contributes to the Kondo resonance disappearance. This process is reversed by a local voltage pulse or thermal annealing to desorb the hydrogen atom.