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The purpose of this study was to determine the acute effect of supplementing with beetroot juice (BRJ) during aerobic exercise. Ten female collegiate volleyball players (mean ±SD: age = 19.3 ±1.3 yr, VO2 peak = 37.4 ±3.3 mL·kg-1·min-1) completed three trials consisting of an initial graded test to exhaustion and two performance trials. For the performance trials, the subjects consumed 60 mL of BRJ (∼4 mmoL of nitrate) or placebo (PL) 3 hrs prior to 5 min of exercising at 45%, 65%, and 85% of volume of oxygen uptake reserve (VO2R). A two-way repeated measures ANOVA was run to determine the effect of treatment and exercise intensity on VO2. Paired samples t-tests were run to compare differences between resting systolic blood pressure (SBP) and diastolic blood pressure (DBP) between treatments. There was no statistically significant interaction between treatment and exercise intensity for VO2. The main effect of treatment was not statistically significant for VO2, (BRJ, 1.32 ±0.53 vs. PL, 1.33 ±0.53 L·min-1, P=0.562). There were no statistically significant differences in resting SBP (BRJ, 110.60 ±6.77 vs. PL; 112.70 ±8.04 mm Hg, P=0.166) or DBP (BRJ, 70.80 ±5.51 vs. PL; 72.30 ±4.83 mm Hg, P=0.285) between treatments. These results indicate that 60 mL of BRJ (∼4 mmoL of nitrate) taken 3 hrs before aerobic exercise had no effect on VO2, SBP, and DBP. [ABSTRACT FROM AUTHOR]