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Cordyceps militaris (CM) is traditionally used as dietary therapy for lung cancer patients in China. CM extract (CME) is hydrosoluble fraction of CM and extensively investigated. Caspase‐3‐involved cell death is considered as its major anticancer mechanism but inconclusive. Therefore, we explore its caspase‐3‐dependent programmed cell death nature (apoptosis and pyroptosis) and validate its caspase‐3‐dependent property in loss‐of‐function experiment. Component profile of CME is detected by High Performance Liquid Chromatography‐ quadrupole time‐of‐flight mass spectrometry (HPLC‐qTOF). Results show that CME causes pyroptosis‐featured cell bubbling and cell lysis and inhibits cell proliferation in A549 cell. CME induces chromatin condensing and makes PI+/annexin V+ staining in bubbling cells, indicating genotoxicity, apoptosis, and pyroptosis cell death are caused by CME. High concentration of CME (200 μg/ml) exerts G2/M and G0 cell cycles arresting and suppresses P53‐downstream proliferative proteins, including P53, P21, CDC25B, CyclinB1, Bcl‐2, and BCL2 associated agonist of cell death (BAD), but 1–100 μg/ml of CME show less effect on proteins above. Correspondingly, caspase‐3 activity and caspase‐3 downstream proteins including pyroptotic effector gasdermin‐E (GSDME) and apoptotic marker cleaved‐poly‐ADP‐ribose polymerase (PARP) are significantly promoted by CME. Moreover, regarding membrane pore formation in pyroptotic cell, expression of membrane GSDME (GSDME antibody conjugated with PE‐Cy7 for detection in flow cytometry) is remarkably increased by CME treatment. By contrast, other pyroptosis‐related proteins such as P2X7, NLRP3, GSDMD, and Caspase‐1 are not affected after CME treatment. Additionally, TET2 is unexpectedly raised by CME. In present of caspase‐3 inhibitor Ac‐DEVD‐CHO (Ac‐DC), CME‐induced cytotoxicity, cell bubbling, and genotoxicity are reduced, and CME‐induced upregulation of apoptosis (cleaved‐PARP‐1) and pyroptosis (GSDME‐NT) proteins are reversed. Lastly, 22 components are identified in HPLC‐qTOF experiment, and they are classified into trophism, neoadjuvant component, cytotoxic component, and cancer deterioration promoter according to previous references. Conclusively, CME causes caspase‐3‐dependent apoptosis and pyroptosis in A549 through caspase‐3/PARP and caspase‐3/GSDME pathways, and it provides basic insight into clinic application of CME for cancer patients., The present study has shown that the Cordyceps militaris extract CME exerts both apoptosis and pyroptosis toward A549 cell line through inducing caspase‐3‐depended pyroptosis and apoptosis.

The aniline in aqueous solutions is harmful to both human health and the environment. A new synthetic, the templated porous carbon, was designed and studied in this study to adsorb the aniline from aqueous solutions. The templated porous carbon was synthesized by SBA-15 and natural zeolite and characterized by SEM/TEM /FTIR and N 2 sorption/desorption isotherm analysis. The results indicated that the adsorption of aniline followed pseudo-second-order kinetic model and was well fit using the Freundlich isotherm model. The adsorption capacity was influenced by the pore structure and pH. The adsorption–desorption cycles were also investigated in this study, and the results suggested the template-synthesized porous carbon would be a promising adsorbent for the removal of aniline from aqueous solution.

Mathematical formula identification is an important part of mathematical formula recognition and retrieval. It is more difficult for extracting formulas from the document images in PDF files because of the diversity of their acquisition ways. To solve the problem, this paper designs a method of mathematical formula identification in English PDF document images, which includes three steps: judging columns, extracting mathematical formula character blocks, merging mathematical formula character blocks. Through analyzing and concluding characteristics of the document images in PDF files as well as its effects on mathematical formula identification, this paper designs a related parameter adjustment algorithm for avoiding influences on the performance of mathematical formula identification caused by the resolution variation. The experimental result shows that the adaptability of mathematical formula identification algorithm is improved by some applications.

A teaching experiment was developed to observing and measuring to cutting process, to improve the teaching performance of the "fundamentals of mechanical manufacturing technology" course. The experiment is carried out on a column milling machine tool; the cutting tool is fixed on the overarm; the board type workpiece is fixed on the worktable. Under the planning fashion at low cutting speed, a CCD microcamera was used to screen from tool orthogonal plane and the orthogonal cutting process can be observed on the display. A transducer was applied to change the speed of worktable with stepless regulation, and the methods of machine tool circuit rebuilding and transducer using were mentioned. So did the buildup of CCD microcamera. The intuitionistic observing to cutting process can be obtained in the experiment, and it can be measuring for rake angle, clearance angle, shear angle, cutting layer and chip thickness, and then the deformation coefficient can be calculated. To the experiment of dynamically observing and measuring to cutting process, the operation is simpleness; the teaching performance is evident; the students feedback is preferably; and the extend value is significant.

To investigate the antiviral and immuno-regulatory effects of Reduning injection on mouse cytomegalovirus (MCMV) pneumonia.BALB/c mice were divided randomly into five groups: blank control group, immunosuppressive control group, MCMV pneumonia group, Reduning treatment group, and ganciclovir treatment group. Acute MCMV interstitial pneumonia models were established in the latter three groups. Lung pathological changes were observed with HE staining, MCMV-DNA content in the lung tissue was detected by qRT-PCR, and the levels of interferon-γ (IFN-γ) and interleukin-6 (IL-6) in the lung tissue were determined by ELISA.Compared with MCMV pneumonia group, the lung injuries in Reduning treatment and ganciclovir treatment groups were ameliorated, and the MCMV-DNA expression in the two treatment groups decreased, and the changes in ganciclovir treatment group were more obvious (P0.05). Compared with MCMV pneumonia group, the levels of IFN-γ and IL-6 in Reduning treatment group rose by 1.4 times and dropped by 30.2%, respectively; and IFN-γ increased by 1.6 times and IL-6 decreased by 47.6% in ganciclovir treatment group (P0.05); the differences between the two treatment groups were statistically significant (P0.05). IFN-γ/IL-6 ratio in Reduning treatment group was higher than that in MCMV pneumonia group, and approached the level of immunosuppressive control group.Reduning injection might exert antiviral activity through inhibiting MCMV replication and proliferation in lung tissue directly, and down-regulating the expressions of IFN-γ and IL-6.

N902 diluted with kerosene was used to co-extract copper and nickel from the ammoniac-carbonate leaching liquor of multi-metal electroplating sludge. The influence factors of the ammoniac-carbonate leaching and the extracting process on the recovery of copper and nickel were studied. The leaching rate of copper and nickel can reach to 90 % and the other metal ions are less leached under the optimal leaching condition. In order to optimize the extraction condition, the L9 (34) intersecting experiment of co-extraction of copper and nickel ions was tested. For a typical ammonium carbonate leaching solution containing Cu2+ 2.23 g/L, Ni2+ 3.31 g/L, Cr