12 results on '"Lin-Jie Shu"'
Search Results
2. β-D-XYLOSIDASE 4 modulates systemic immune signaling in Arabidopsis thaliana
- Author
-
Kornelia Bauer, Shahran Nayem, Martin Lehmann, Marion Wenig, Lin-Jie Shu, Stefanie Ranf, Peter Geigenberger, and A. Corina Vlot
- Subjects
plant immunity ,plant defense ,systemic acquired resistance ,cell wall ,xylosidase ,xylose ,Plant culture ,SB1-1110 - Abstract
Pectin- and hemicellulose-associated structures of plant cell walls participate in defense responses against pathogens of different parasitic lifestyles. The resulting immune responses incorporate phytohormone signaling components associated with salicylic acid (SA) and jasmonic acid (JA). SA plays a pivotal role in systemic acquired resistance (SAR), a form of induced resistance that - after a local immune stimulus - confers long-lasting, systemic protection against a broad range of biotrophic invaders. β-D-XYLOSIDASE 4 (BXL4) protein accumulation is enhanced in the apoplast of plants undergoing SAR. Here, two independent Arabidopsis thaliana mutants of BXL4 displayed compromised systemic defenses, while local resistance responses to Pseudomonas syringae remained largely intact. Because both phloem-mediated and airborne systemic signaling were abrogated in the mutants, the data suggest that BXL4 is a central component in SAR signaling mechanisms. Exogenous xylose, a possible product of BXL4 enzymatic activity in plant cell walls, enhanced systemic defenses. However, GC-MS analysis of SAR-activated plants revealed BXL4-associated changes in the accumulation of certain amino acids and soluble sugars, but not xylose. In contrast, the data suggest a possible role of pectin-associated fucose as well as of the polyamine putrescine as regulatory components of SAR. This is the first evidence of a central role of cell wall metabolic changes in systemic immunity. Additionally, the data reveal a so far unrecognized complexity in the regulation of SAR, which might allow the design of (crop) plant protection measures including SAR-associated cell wall components.
- Published
- 2023
- Full Text
- View/download PDF
3. Identification of a strawberry NPR-like gene involved in negative regulation of the salicylic acid-mediated defense pathway.
- Author
-
Lin-Jie Shu, Jui-Yu Liao, Nai-Chun Lin, and Chia-Lin Chung
- Subjects
Medicine ,Science - Abstract
Hormonal modulation plays a central role in triggering various resistant responses to biotic and abiotic stresses in plants. In cultivated strawberry (Fragaria x ananassa), the salicylic acid (SA)-dependent defense pathway has been associated with resistance to Colletotrichum spp. and the other pathogens. To better understand the SA-mediated defense mechanisms in strawberry, we analyzed two strawberry cultivars treated with SA for their resistance to anthracnose and gene expression profiles at 6, 12, 24, and 48 hr post-treatment. Strawberry genes related to SA biosynthesis, perception, and signaling were identified from SA-responsive transcriptomes of the two cultivars, and the induction of 17 candidate genes upon SA treatment was confirmed by qRT-PCR. Given the pivotal role of the non-expressor of pathogenesis-related (NPR) family in controlling the SA-mediated defense signaling pathway, we then analyzed NPR orthologous genes in strawberry. From the expression profile, FaNPRL-1 [ortholog of FvNPRL-1 (gene20070 in F. vesca)] was identified as an NPR-like gene significantly induced after SA treatment in both cultivars. With a conserved BTB/POZ domain, ankyrin repeat domain, and nuclear localization signal, FvNPRL-1 was found phylogenetically closer to NPR3/NPR4 than NPR1 in Arabidopsis. Ectopic expression of FvNPRL-1 in the Arabidopsis thaliana wild type suppressed the SA-mediated PR1 expression and the resistance to Pseudomonas syringae pv. tomato DC3000. Transient expression of FvNPRL-1 fused with green fluorescent protein in Arabidopsis protoplasts showed that SA affected nuclear translocation of FvNPRL-1. FvNPRL-1 likely functions similar to Arabidopsis NPR3/NPR4 as a negative regulator of the SA-mediated defense.
- Published
- 2018
- Full Text
- View/download PDF
4. β-D-XYLOSIDASE 4 modulates systemic immune signaling in Arabidopsis thaliana
- Author
-
Kornelia Bauer, Shahran Nayem, Martin Lehmann, Marion Wenig, Lin-Jie Shu, Stefanie Ranf, Peter Geigenberger, and A. Corina Vlot
- Subjects
Plant Science ,plant immunity ,plant defense ,systemic acquired resistance ,cell wall ,xylosidase ,xylose ,fucose ,putrescine ,ddc - Abstract
Pectin- and hemicellulose-associated structures of plant cell walls participate in defense responses against pathogens of different parasitic lifestyles. The resulting immune responses incorporate phytohormone signaling components associated with salicylic acid (SA) and jasmonic acid (JA). SA plays a pivotal role in systemic acquired resistance (SAR), a form of induced resistance that - after a local immune stimulus - confers long-lasting, systemic protection against a broad range of biotrophic invaders. β-D-XYLOSIDASE 4 (BXL4) protein accumulation is enhanced in the apoplast of plants undergoing SAR. Here, two independent Arabidopsis thaliana mutants of BXL4 displayed compromised systemic defenses, while local resistance responses to Pseudomonas syringae remained largely intact. Because both phloem-mediated and airborne systemic signaling were abrogated in the mutants, the data suggest that BXL4 is a central component in SAR signaling mechanisms. Exogenous xylose, a possible product of BXL4 enzymatic activity in plant cell walls, enhanced systemic defenses. However, GC-MS analysis of SAR-activated plants revealed BXL4-associated changes in the accumulation of certain amino acids and soluble sugars, but not xylose. In contrast, the data suggest a possible role of pectin-associated fucose as well as of the polyamine putrescine as regulatory components of SAR. This is the first evidence of a central role of cell wall metabolic changes in systemic immunity. Additionally, the data reveal a so far unrecognized complexity in the regulation of SAR, which might allow the design of (crop) plant protection measures including SAR-associated cell wall components.
- Published
- 2022
5. β-D-XYLOSIDASE 4 modulates systemic immune signaling in Arabidopsis thaliana.
- Author
-
Bauer, Kornelia, Nayem, Shahran, Lehmann, Martin, Wenig, Marion, Lin-Jie Shu, Ranf, Stefanie, Geigenberger, Peter, and Corina Vlot, A.
- Subjects
HEMICELLULOSE ,PECTINS ,PLANT cell walls ,ARABIDOPSIS thaliana ,PLANT anatomy ,PSEUDOMONAS syringae ,SALICYLIC acid ,JASMONIC acid ,ARABIDOPSIS - Abstract
Pectin- and hemicellulose-associated structures of plant cell walls participate in defense responses against pathogens of different parasitic lifestyles. The resulting immune responses incorporate phytohormone signaling components associated with salicylic acid (SA) and jasmonic acid (JA). SA plays a pivotal role in systemic acquired resistance (SAR), a form of induced resistance that - after a local immune stimulus - confers long-lasting, systemic protection against a broad range of biotrophic invaders. β-D-XYLOSIDASE 4 (BXL4) protein accumulation is enhanced in the apoplast of plants undergoing SAR. Here, two independent Arabidopsis thaliana mutants of BXL4 displayed compromised systemic defenses, while local resistance responses to Pseudomonas syringae remained largely intact. Because both phloem-mediated and airborne systemic signaling were abrogated in the mutants, the data suggest that BXL4 is a central component in SAR signaling mechanisms. Exogenous xylose, a possible product of BXL4 enzymatic activity in plant cell walls, enhanced systemic defenses. However, GC-MS analysis of SAR-activated plants revealed BXL4-associated changes in the accumulation of certain amino acids and soluble sugars, but not xylose. In contrast, the data suggest a possible role of pectin-associated fucose as well as of the polyamine putrescine as regulatory components of SAR. This is the first evidence of a central role of cell wall metabolic changes in systemic immunity. Additionally, the data reveal a so far unrecognized complexity in the regulation of SAR, which might allow the design of (crop) plant protection measures including SAR-associated cell wall components. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
6. Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms
- Author
-
Sandrine Dhondt-Cordelier, Jérôme Crouzet, Sandra Villaume, Stéphan Dorey, Sylvain Cordelier, Fabienne Baillieul, Corinna Dawid, Jean-Hugues Renault, Stefanie Ranf, Cyril Zipfel, Christophe Clément, Eric Déziel, Maude Cloutier, Alexander Kutschera, Charles Gauthier, Arvin Nickzad, Tim Gerster, Jane Hubert, Arnaud Haudrechy, Lin-Jie Shu, Marc Ongena, Florence Mazeyrat-Gourbeyre, Romain Schellenberger, Christian Schmid, Nicolas Borie, Thomas Hofmann, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), NatExplore, and University of Zurich
- Subjects
0106 biological sciences ,0303 health sciences ,Innate immune system ,Multidisciplinary ,biology ,Chemistry ,Pseudomonas ,Plant Immunity ,580 Plants (Botany) ,biology.organism_classification ,01 natural sciences ,Microbiology ,03 medical and health sciences ,Immune system ,10126 Department of Plant and Microbial Biology ,Immunity ,Arabidopsis ,mental disorders ,Plant defense against herbivory ,Arabidopsis thaliana ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[CHIM]Chemical Sciences ,10211 Zurich-Basel Plant Science Center ,030304 developmental biology ,010606 plant biology & botany - Abstract
International audience; Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from l -rhamnose and ( R )-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial surface motility, biofilm development, and thus successful colonization of hosts. Here, we show that the lipidic secretome from the opportunistic pathogen Pseudomonas aeruginosa , mainly comprising RLs and HAAs, stimulates Arabidopsis immunity. We demonstrate that HAAs are sensed by the bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29), which also mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) perception, in the plant Arabidopsis thaliana . HAA sensing induces canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, although abolishing sensing by LORE, do not impair their ability to trigger plant defense. Moreover, our results show that the immune response triggered by RLs is affected by the sphingolipid composition of the plasma membrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms.
- Published
- 2021
7. Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate
- Author
-
Romain, Schellenberger, Jérôme, Crouzet, Arvin, Nickzad, Lin-Jie, Shu, Alexander, Kutschera, Tim, Gerster, Nicolas, Borie, Corinna, Dawid, Maude, Cloutier, Sandra, Villaume, Sandrine, Dhondt-Cordelier, Jane, Hubert, Sylvain, Cordelier, Florence, Mazeyrat-Gourbeyre, Christian, Schmid, Marc, Ongena, Jean-Hugues, Renault, Arnaud, Haudrechy, Thomas, Hofmann, Fabienne, Baillieul, Christophe, Clément, Cyril, Zipfel, Charles, Gauthier, Eric, Déziel, Stefanie, Ranf, and Stéphan, Dorey
- Subjects
Arabidopsis Proteins ,Arabidopsis ,Pseudomonas syringae ,Protein Serine-Threonine Kinases ,Biological Sciences ,Plants, Genetically Modified ,Immunity, Innate ,mental disorders ,Host-Pathogen Interactions ,Tobacco ,Plant Immunity ,Calcium Signaling ,Glycolipids ,Phosphorylation ,Reactive Oxygen Species ,Disease Resistance ,Plant Diseases - Abstract
Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from l-rhamnose and (R)-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial surface motility, biofilm development, and thus successful colonization of hosts. Here, we show that the lipidic secretome from the opportunistic pathogen Pseudomonas aeruginosa, mainly comprising RLs and HAAs, stimulates Arabidopsis immunity. We demonstrate that HAAs are sensed by the bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29), which also mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) perception, in the plant Arabidopsis thaliana. HAA sensing induces canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, although abolishing sensing by LORE, do not impair their ability to trigger plant defense. Moreover, our results show that the immune response triggered by RLs is affected by the sphingolipid composition of the plasma membrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms.
- Published
- 2021
8. Multi-omics approach to identify bacterial polyynes and unveil their antifungal mechanism against Candida albicans
- Author
-
Ching-Chih Lin, Sin Yong Hoo, Chih Lin, Kai-Fa Huang, Ying-Ning Ho, Chi-Hui Sun, Han-Jung Lee, Pi-Yu Chen, Lin-Jie Shu, Bo-Wei Wang, Wei-Chen Hsu, and Yu-Liang Yang
- Subjects
Comparative genomics ,chemistry.chemical_compound ,Metabolomics ,Biosynthesis ,Biochemistry ,biology ,Chemistry ,Acetyltransferase ,Gene cluster ,Antimicrobial ,Candida albicans ,biology.organism_classification ,Gene - Abstract
Bacterial polyynes are highly active natural products with a broad-spectrum of antimicrobial activities. However, their detailed mechanism of action remains unclear. Through integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified a unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), from the biosynthesis gene cluster (BGC) dominant for the production of antifungal polyynes (massilin A, massilin B, collimonin C, and collimonin D) in Massilia sp. YMA4. Phylogenic and chemotaxonomic analyses characterized the core architecture of bacterial polyyne BGC. The crystallographic analysis of the MasL-collimonin C complex indicated that bacterial polyynes serve as a covalent inhibitor of acetyl-CoA acetyltransferase. Moreover, we confirmed that the bacterial polyynes disrupted cell membrane integrity and inhibited cell viability of Candida albicans by targeting ERG10 (homolog of MasL). Overall, understanding of the antifungal mechanism of bacterial polyynes presented herein will be useful for the development of polyynes for fungal infections.
- Published
- 2021
9. Low cost, medium throughput depletion-binding assay for screening S-domain-receptor ligand interactions using in planta protein expression
- Author
-
Lin-Jie Shu, Milena Schäffer, Sabine Eschrig, Stefanie Ranf and Lehrstuhl für Phytopathologie
- Subjects
ddc:630 ,ddc - Published
- 2020
10. Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms.
- Author
-
Schellenberger, Romain, Crouzet, Jérôme, Nickzad, Arvin, Lin-Jie Shu, Kutschera, Alexander, Gerster, Tim, Borie, Nicolas, Dawid, Corinna, Cloutier, Maude, Villaume, Sandra, Dhondt-Cordelier, Sandrine, Hubert, Jane, Cordelier, Sylvain, Mazeyrat-Gourbeyre, Florence, Schmid, Christian, Ongena, Marc, Renault, Jean-Hugues, Haudrechy, Arnaud, Hofmann, Thomas, and Baillieul, Fabienne
- Subjects
RHAMNOLIPIDS ,PSEUDOMONAS diseases ,ARABIDOPSIS ,COLONIZATION (Ecology) ,DISEASE resistance of plants - Abstract
Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from L-rhamnose and (R)-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial surface motility, biofilm development, and thus successful colonization of hosts. Here, we show that the lipidic secretome from the opportunistic pathogen Pseudomonas aeruginosa, mainly comprising RLs and HAAs, stimulates Arabidopsis immunity. We demonstrate that HAAs are sensed by the bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29), which also mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) perception, in the plant Arabidopsis thaliana. HAA sensing induces canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, although abolishing sensing by LORE, do not impair their ability to trigger plant defense. Moreover, our results show that the immune response triggered by RLs is affected by the sphingolipid composition of the plasmamembrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
11. Imaging mass spectrometry for metabolites: technical progress, multimodal imaging, and biological interactions
- Author
-
Lin-Jie Shu, Yu-Liang Yang, and Ying-Ning Ho
- Subjects
0301 basic medicine ,Multimodal imaging ,Computer science ,010401 analytical chemistry ,Medicine (miscellaneous) ,Bioinformatics ,Mass spectrometry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Mass spectrometry imaging ,Mass Spectrometry ,0104 chemical sciences ,Molecular Imaging ,Matrix (chemical analysis) ,03 medical and health sciences ,030104 developmental biology ,Tissue sections ,Molecular level ,Animals ,Humans ,Biochemical engineering ,Analysis tools ,Molecular imaging - Abstract
Imaging mass spectrometry (IMS) allows the study of the spatial distribution of small molecules in biological samples. IMS is able to identify and quantify chemicals in situ from whole tissue sections to single cells. Both vacuum mass spectrometry (MS) and ambient MS systems have advanced considerably over the last decade; however, some limitations are still hard to surmount. Sample pretreatment, matrix or solvent choices, and instrument improvement are the key factors that determine the successful application of IMS to different samples and analytes. IMS with innovative MS analyzers, powerful MS spectrum databases, and analysis tools can efficiently dereplicate, identify, and quantify natural products. Moreover, multimodal imaging systems and multiple MS-based systems provide additional structural, chemical, and morphological information and are applied as complementary tools to explore new fields. IMS has been applied to reveal interactions between living organisms at molecular level. Recently, IMS has helped solve many previously unidentifiable relations between bacteria, fungi, plants, animals, and insects. Other significant interactions on the chemical level can also be resolved using expanding IMS techniques. WIREs Syst Biol Med 2017, 9:e1387. doi: 10.1002/wsbm.1387 For further resources related to this article, please visit the WIREs website.
- Published
- 2016
12. Identification of a strawberry NPR-like gene involved in negative regulation of the salicylic acid-mediated defense pathway
- Author
-
Chia-Lin Chung, Jui-Yu Liao, Nai-Chun Lin, and Lin-Jie Shu
- Subjects
0106 biological sciences ,0301 basic medicine ,Leaves ,Arabidopsis ,lcsh:Medicine ,Gene Expression ,Plant Science ,Biochemistry ,01 natural sciences ,Plant Growth Regulators ,Gene expression ,Pseudomonas syringae ,Arabidopsis thaliana ,Plant Immunity ,lcsh:Science ,Phylogeny ,Disease Resistance ,Plant Proteins ,Multidisciplinary ,biology ,Plant Anatomy ,Eukaryota ,Genomics ,Plants ,Plants, Genetically Modified ,NPR1 ,Cell biology ,Chemistry ,Experimental Organism Systems ,Physical Sciences ,Salicylic Acid ,Transcriptome Analysis ,Research Article ,Arabidopsis Thaliana ,Brassica ,Research and Analysis Methods ,Genes, Plant ,Green Fluorescent Protein ,Fragaria ,03 medical and health sciences ,Model Organisms ,Plant and Algal Models ,Gene Types ,DNA-binding proteins ,Genetics ,Gene Regulation ,Gene ,Plant Diseases ,lcsh:R ,fungi ,Organisms ,Chemical Compounds ,Wild type ,Biology and Life Sciences ,Proteins ,Computational Biology ,Genome Analysis ,biology.organism_classification ,Regulatory Proteins ,Luminescent Proteins ,030104 developmental biology ,Animal Studies ,Regulator Genes ,lcsh:Q ,Ectopic expression ,Transcriptome ,Acids ,Transcription Factors ,010606 plant biology & botany - Abstract
Hormonal modulation plays a central role in triggering various resistant responses to biotic and abiotic stresses in plants. In cultivated strawberry (Fragaria x ananassa), the salicylic acid (SA)-dependent defense pathway has been associated with resistance to Colletotrichum spp. and the other pathogens. To better understand the SA-mediated defense mechanisms in strawberry, we analyzed two strawberry cultivars treated with SA for their resistance to anthracnose and gene expression profiles at 6, 12, 24, and 48 hr post-treatment. Strawberry genes related to SA biosynthesis, perception, and signaling were identified from SA-responsive transcriptomes of the two cultivars, and the induction of 17 candidate genes upon SA treatment was confirmed by qRT-PCR. Given the pivotal role of the non-expressor of pathogenesis-related (NPR) family in controlling the SA-mediated defense signaling pathway, we then analyzed NPR orthologous genes in strawberry. From the expression profile, FaNPRL-1 [ortholog of FvNPRL-1 (gene20070 in F. vesca)] was identified as an NPR-like gene significantly induced after SA treatment in both cultivars. With a conserved BTB/POZ domain, ankyrin repeat domain, and nuclear localization signal, FvNPRL-1 was found phylogenetically closer to NPR3/NPR4 than NPR1 in Arabidopsis. Ectopic expression of FvNPRL-1 in the Arabidopsis thaliana wild type suppressed the SA-mediated PR1 expression and the resistance to Pseudomonas syringae pv. tomato DC3000. Transient expression of FvNPRL-1 fused with green fluorescent protein in Arabidopsis protoplasts showed that SA affected nuclear translocation of FvNPRL-1. FvNPRL-1 likely functions similar to Arabidopsis NPR3/NPR4 as a negative regulator of the SA-mediated defense.
- Published
- 2018
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