Search

Additional file 1: SupplementaryFigure 1. Copy number variation and expression of EGFR in Kyse30and FaDu cells. Supplementary Figure 2. GSEA of EGF- and EpEX-treated Kyse30and FaDu cells. Supplementary Figure 3. Over-representation analysis of genesof the EGFR-mediated EMT signature. SupplementaryFigure 4. Comparison ofEGFR-mediated EMT, pEMT, and EMT signatures. Supplementary Figure 5. Comparisonof EMT signatures for prognostic purposes.Supplementary Figure 6. ITGB4,ITGA6, LAMA3, LAMB3, and LAMC2 expression in HNSCC. Supplementary Figure 7. ITGB4expression in malignant and non-malignant single cells in different cancerentities. Supplementary Figure 8. ITGA6 expression in malignant andnon-malignant single cells in different cancer entities. Supplementary Figure 9. LAMA3expression in malignant and non-malignant single cells in different cancerentities. Supplementary Figure 10. LAMB3 expression in malignant andnon-malignant single cells in different cancer entities. Supplementary Figure 11. LAMC2expression in malignant and non-malignant single cells in different cancerentities. Supplementary Figure 12. ITGB4 expression in knockdown clonesof Kyse30 and FaDu cells. Supplementary Figure 13. Wound healing capacity of control andITGB4-knockdown cell lines. SupplementaryFigure 14. Tumor buddingintensities in HNSCC.

Additional file 4: Supplementary Table 3. Gene expression correlation with ITGB4 in the HPV-negativeTCGA cohort. Batch correlation analysis identified correlations of geneexpression with integrin beta 4 (ITGB4). Gene ID, Spearman correlation, andp-value are indicated for the top ten positively (co-regulated) and negativelycorrelated genes (counterregulated).

An investigation of the distribution and size composition of chlorophyll a concentration was carried out in the Southwestern Indian Ocean(47—70°E,25—40°S) in January 2011,and factors that might affect the distribution and size composition were discussed.Fluorometric method was used to obtain total and size-fractioned chlorophyll a concentrations.The survey was carried out during the austral summer,and there were 6 stations altogether,two of which located in the region of the south Indian Ocean Subtropical Gyre(IOSG) and the other four located in the subtropical convergence zone(SCZ).Water samples were collected from 13 layers in 0—200m column of each station and the vertical profiles of chlorophyll a,nutrients(NO-3 、PO3-4 and SiO2-3),temperature and salinity were obtained.The objective of this study was to investigate the spatial distribution and size fraction of chlorophyll a concentration and to relate them to environmental factors.The results showed that the surface chlorophyll a concentration was low and did not exceed 0.07mg/m3,and the depth of the subsurface chlorophyll a maximum was deep,at approximately 100m and sometimes reaching as deep as 150m in the region of IOSG;However,in the SCZ,the surface chlorophyll a concentration was higher(0.164—0.247 mg/m3),and the subsurface chlorophyll a maximum layer occurred between 50—70m.In the region of IOSG,phytoplankton growth is limited by nutrients supply,while the frontal system formed by the Agulhas Return Current(ARC) and the Subtropical Convergence(STC) is a region of intense mesoscale activity presenting enhanced levels of biological production and chlorophyll a concentration.Based on the discussion of the factors affecting the distribution of phytoplankton,it appeared that NO-3 was the main factor that limited the growth of phytoplankton in the southwestern Indian Ocean,and PO3-4 could be the main limiting factor in some areas.Size-fractioned chlorophyll a concentration clearly showed that the study area was a typical oligotrophic open ocean.The picophytoplankton(0.2—2μm) chlorophyll a always dominated,representing 71.1% of the total chlorophyll a concentration on average,while the average contribution of nanophytoplankton(2—20μm) and microphytoplankton(20μm) chlorophyll a to total chlorophyll a concentration were 24.2% and 4.7% respectively.The dominance of picophytoplankton was resulted from the low nutrient concentration environment because picophytoplankton were more competent over the microphytoplankton and nanophytoplankton under oligotrophic condition.There were some differences on the contribution of picophytoplankton to total chlorophyll a concentration between the regions of IOSG and SCZ,which were 77.9% and 66.7% respectively.The differences of the hydrographical characteristics,which in turn influenced the nutrient dynamics in the region of IOSG and SCZ,might lead to the significant difference of the spatial distribution and size composition of chlorophyll a concentration between these two regions.