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4. Establishment and Characterization of a Chicken Myoblast Cell Line.

Author

Guo, Dongxue, Lin, Shudai, Wang, Xiaotong, Jiao, Zhenhai, Li, Guo, An, Lilong, Zhang, Zihao, and Zhang, Li

Subjects
*TELOMERASE reverse transcriptase, *CELL fusion, *MUSCLE growth, *CELL differentiation, *EMBRYOLOGY
Abstract

Skeletal muscle, which is predominantly constituted by multinucleated muscle fibers, plays a pivotal role in sustaining bodily movements and energy metabolism. Myoblasts, which serve as precursor cells for differentiation and fusion into muscle fibers, are of critical importance in the exploration of the functional genes associated with embryonic muscle development. However, the in vitro proliferation of primary myoblasts is inherently constrained. In this study, we achieved a significant breakthrough by successfully establishing a chicken myoblast cell line through the introduction of the exogenous chicken telomerase reverse transcriptase (chTERT) gene, followed by rigorous G418-mediated pressure screening. This newly developed cell line, which was designated as chTERT-myoblasts, closely resembled primary myoblasts in terms of morphology and exhibited remarkable stability in culture for at least 20 generations of population doublings without undergoing malignant transformation. In addition, we conducted an exhaustive analysis that encompassed cellular proliferation, differentiation, and transfection characteristics. Our findings revealed that the chTERT-myoblasts had the ability to proliferate, differentiate, and transfect after multiple rounds of population doublings. This achievement not only furnished a valuable source of homogeneous avian cell material for investigating embryonic muscle development, but also provided valuable insights and methodologies for establishing primary cell lines. [ABSTRACT FROM AUTHOR]

Published
2024
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14. List of contributors

Author

Agarwaal, Viplove, primary, Annacondia, Maria Luz, additional, Baruah, Kartik, additional, Basu, Amitava, additional, Ben Maamar, Millissia, additional, Bossier, Peter, additional, Casati, Lavinia, additional, Cavalieri, Vincenzo, additional, Celotti, Fabio, additional, Champagne, Frances A., additional, D'Cruz, Shereen Cynthia, additional, Diaz, Ruben, additional, Dwivedi, Ambey Prasad, additional, Fang, Lingzhao, additional, Freeman, Dana M., additional, Ge, Zhao-Jia, additional, Ho, Dao H., additional, Jiménez-Chillarón, Josep C., additional, Khosla, Sanjeev, additional, Legoff, Louis, additional, Lerin, Carles, additional, Li, Cong-Jun, additional, Lin, Shudai, additional, Liu, George E., additional, Martinez, German, additional, Mashoodh, Rahia, additional, Meyer-Ficca, Mirella L., additional, Meyer, Ralph G., additional, Mukherjee, Krishnendu, additional, Nilsson, Eric, additional, Norouzitallab, Parisa, additional, Nowak, Felicia V., additional, Palacios-Marin, Ivonne, additional, Rajeev, Ramisetti, additional, Ramon-Krauel, Marta, additional, Ribo, Silvia, additional, Riddle, Nicole C., additional, Schoelz, John M., additional, Sijbrands, Eric J.G., additional, Sinha, Anunay, additional, Skinner, Michael K., additional, Slyvka, Yuriy, additional, Smagulova, Fatima, additional, Spinelli, Giovanni, additional, Stuppia, Liborio, additional, Sun, Qing-Yuan, additional, Tevosian, Sergei, additional, Thamban, Thushara, additional, Tollefsbol, Trygve O., additional, Uller, Tobias, additional, Vanrompay, Daisy, additional, Varriale, Annalisa, additional, Vilcinskas, Andreas, additional, and Wang, Zhibin, additional

Published
2019
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18. Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer.

Author

Liang, Keying, Wang, Qian, Qiu, Li, Gong, Xiaocheng, Chen, Zixi, Zhang, Haibo, Ding, Ke, Liu, Yunfei, Wei, Jinfen, Lin, Shudai, Fu, Shuying, and Du, Hongli

Subjects
CELL cycle, CELL proliferation, UBIQUITIN-conjugating enzymes, GENE expression, CELL migration, WESTERN immunoblotting
Abstract

Various studies have shown that the cell-cycle-related regulatory proteins UBE2C, PLK1, and BIRC5 promote cell proliferation and migration in different types of cancer. However, there is a lack of in-depth and systematic research on the mechanism of these three as therapeutic targets. In this study, we found a positive correlation between the expression of UBE2C and PLK1/BIRC5 in the Cancer Genome Atlas (TCGA) database, revealing a potential combination therapy candidate for pan-cancer. Quantitative real-time PCR (qRT-PCR), Western blotting (WB), cell phenotype detection, and RNA-seq techniques were used to evidence the effectiveness of the combination candidate. We found that combined interference of UBE2C with PLK1 and UBE2C with BIRC5 affected metabolic pathways by significantly downregulating the mRNA expression of IDH1 and ACLY, which was related to the synthesis of acetyl-CoA. By combining the PLK1 inhibitor volasertib and the ACLY inhibitor bempedoic acid, it showed a higher synergistic inhibition of cell viability and higher synergy scores in seven cell lines, compared with those of other combination treatments. Our study reveals the potential mechanisms through which cell-cycle-related genes regulate metabolism and proposes a potential combined targeted therapy for patients with higher PLK1 and ACLY expression in pan-cancer. [ABSTRACT FROM AUTHOR]

Published
2023
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21. KAT2A/E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer

Author

Lin, Shudai, primary, Qiu, Li, additional, Liang, Keying, additional, Zhang, Haibo, additional, Xian, Mingjian, additional, Chen, Zixi, additional, Wei, Jinfen, additional, Fu, Shuying, additional, Gong, Xiaocheng, additional, Ding, Ke, additional, Zhang, Zihao, additional, Hu, Bowen, additional, Zhang, Xiquan, additional, Duan, Yuyou, additional, and Du, Hongli, additional

Published
2022
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24. The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts.

Author

Zhang, Weilu, Lin, Shudai, Jiao, Zhenhai, An, Lilong, Xie, Tingting, Wu, Jiang, and Zhang, Li

Subjects
*SOMATOTROPIN receptors, *ANTERIOR pituitary gland, *LIVER regeneration, *LIVER cells, *INHIBITION of cellular proliferation, *MYOBLASTS, *WNT signal transduction
Abstract

The anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH–GHR–IGF1 signaling pathway. Therefore, the amount of GHR and the integrity of its structure will affect animal growth and development. In the previous study, we found that the mouse GHR gene can transcribe a circular transcript named circGHR. Our group cloned the full-length of the mouse circGHR and analyzed its spatiotemporal expression profile. In this study, we further predicted the open reading frame of circGHR with bioinformatics, subsequently constructed a Flag-tagged protein vector and preliminarily verified its coding potential with western blot. Additionally, we found that circGHR could inhibit the proliferation of NCTC469 cells and has a tendency to inhibit cell apoptosis, while for C2C12 cells, it showed a tendency to inhibit cell proliferation and promote its differentiation. Overall, these results suggested that the mouse circGHR had the potential to encode proteins and affect cell proliferation, differentiation and apoptosis. [ABSTRACT FROM AUTHOR]

Published
2023
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25. PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer

Author

Zhang, Haibo, primary, He, Ziqing, additional, Qiu, Li, additional, Wei, Jinfen, additional, Gong, Xiaocheng, additional, Xian, Mingjian, additional, Chen, Zixi, additional, Cui, Ying, additional, Fu, Shuying, additional, Zhang, Zihao, additional, Hu, Bowen, additional, Zhang, Xiquan, additional, Lin, Shudai, additional, and Du, Hongli, additional

Published
2022
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26. Supplemental Material - Identification and Validation of Serum CST1 as a Diagnostic Marker for Differentiating Early-Stage Non-Small Cell Lung Cancer from Pulmonary Benign Nodules

Author

Lai, Yanzhen, Wang, Yu, Wu, Yaxian, Wu, Meng, Xing, Shan, Xie, Ying, Chen, Shulin, Li, Xiaohui, Zhang, Ao, He, Yi, Li, Huilan, Dai, Shuqin, Wang, Junye, Lin, Shudai, Bai, Yunmeng, Du, Hongli, and Liu, Wanli

Subjects
viruses, FOS: Clinical medicine, 111299 Oncology and Carcinogenesis not elsewhere classified
Abstract

Supplemental Material for Identification and Validation of Serum CST1 as a Diagnostic Marker for Differentiating Early-Stage Non-Small Cell Lung Cancer from Pulmonary Benign Nodules by Yanzhen Lai, Yu Wang, Yaxian Wu, Meng Wu, Shan Xing, Ying Xie, Shulin Chen, Xiaohui Li, Ao Zhang, Yi He, Huilan Li, Shuqin Dai, Junye Wang, Shudai Lin, Yunmeng Bai, Hongli Du, and Wanli Liu in Cancer Control

Published
2022
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27. Identification and Validation of Serum CST1 as a Diagnostic Marker for Differentiating Early-Stage Non-Small Cell Lung Cancer from Pulmonary Benign Nodules

Author

Lai, Yanzhen, primary, Wang, Yu, additional, Wu, Yaxian, additional, Wu, Meng, additional, Xing, Shan, additional, Xie, Ying, additional, Chen, Shulin, additional, Li, Xiaohui, additional, Zhang, Ao, additional, He, Yi, additional, Li, Huilan, additional, Dai, Shuqin, additional, Wang, Junye, additional, Lin, Shudai, additional, Bai, Yunmeng, additional, Du, Hongli, additional, and Liu, Wanli, additional

Published
2022
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28. KAT2A / E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer.

Author

Lin, Shudai, Qiu, Li, Liang, Keying, Zhang, Haibo, Xian, Mingjian, Chen, Zixi, Wei, Jinfen, Fu, Shuying, Gong, Xiaocheng, Ding, Ke, Zhang, Zihao, Hu, Bowen, Zhang, Xiquan, Duan, Yuyou, and Du, Hongli

Subjects
*UBIQUITIN-conjugating enzymes, *CELL migration, *CELL proliferation, *CANCER cell migration, *CELL cycle, *UBIQUITINATION, *WESTERN immunoblotting
Abstract

Various studies have shown that lysine acetyltransferase 2A (KAT2A), E2F transcription factor 1 (E2F1), and ubiquitin conjugating enzyme E2 C (UBE2C) genes regulated the proliferation and migration of tumor cells through regulating the cell cycle. However, there is a lack of in-depth and systematic research on their mechanisms of action. This study analyzed The Cancer Genome Atlas (TCGA) to screen potential candidate genes and the regulation network of KAT2A and E2F1 complex in pan-cancer. Quantitative real-time PCR (qRT-PCR) and Western blotting (WB), cell phenotype detection, immunofluorescence co-localization, chromatin immunoprecipitation assay (ChIP), and RNA-Seq techniques were used to explore the functional of a candidate gene, UBE2C. We found that the expression of these three genes was significantly higher in more than 10 tumor types compared to normal tissue. Moreover, UBE2C was mainly expressed in tumor cells, which highlighted the impacts of UBE2C as a specific therapeutic strategy. Moreover, KAT2A and E2F1 could promote cell proliferation and the migration of cancer cells by enhancing the expression of UBE2C. Mechanically, KAT2A was found to cooperate with E2F1 and be recruited by E2F1 to the UBE2C promoter for elevating the expression of UBE2C by increasing the acetylation level of H3K9. [ABSTRACT FROM AUTHOR]

Published
2022
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31. Evolution and molecular characteristics of SARS-CoV-2 genome

Author

Bai, Yunmeng, Jiang, Dawei, Lon, Jerome R, Chen, Xiaoshi, Hu, Meiling, Lin, Shudai, Chen, Zixi, Meng, Yuhuan, and Du, Hongli

Subjects
viruses, skin and connective tissue diseases
Abstract

In the evolution analysis of 622 complete human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes with high quality, the estimated Ka/Ks ratio of SARS-CoV-2 is 1.008, which is significantly higher than that of SARS-CoV and MERS-CoV, and the time to the most recent common ancestor (tMRCA) of SARS-CoV-2 is inferred in late September 2019 (95% CI: 2019/08/28-2019/10/26), which indicating that SARS-CoV-2 may have completed a positive selection pressure of the cross-host evolution in the early stage and be going through a neutral evolution at present. In addition, no-root phylogenetic tree of the 622 SARS-CoV-2 genomes were constructed by maximum likelihood (ML) with the bootstrap value of 100. According to the phylogenetic trees, all genomes were divided into Cluster 1 to 3, in which genomes were mainly from North America, global and Europe respectively. Further we find 9 key specific sites of highly linkage which play a decisive role in the classification of each cluster. Among them, 3 and 4 sites of almost complete linkage are the specific sites for Cluster 1 and Cluster 3 respectively. Notably the frequencies of haplotype TTTG and H1 are generally high in European countries and correlated to death rate (r>0.4) based on more than 3500 SARS-CoV-2 genomes, which indicated that the haplotypes might be related to pathogenicity of SARS-CoV-2 and need to be addressed. According to haplotype changes in chronological order, the H3 haplotype subgroup disappeared soon after detection, while H1 haplotype subgroup was globally increasing with time. The evolution and molecular characteristics of more than 3500 genomic sequences provided a new perspective for revealing the epidemiology mechanism of SARS-CoV-2 and coping with SARS-CoV-2 effectively.

Published
2020
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