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Generic data envelopment analysis (DEA) models are based on deterministic input and output. However, input and output vectors are often interrupted by random factors, such as measurement errors and data noise, in real economic situations. This study proposes a new chance-constrained network DEA model based on the modified directional distance function (DDF) and enhanced Russell measure (ERM) model for assessing government management and culture-led urban regeneration. In addition to exploring the randomness of data, this study integrates the advantages of both ERM and DDF and considers the inefficiency level from a non-oriented viewpoint, the direction vector, and each input and output simultaneously. Each input and output of the two production stages can use non-radials to measure efficiency. Results show that the urban-rural gap has gradually widened since 2015. To validate the legitimacy of the model, this study utilizes the bootstrapping method to verify the results of the stochastic network DEA model and the conventional two-stage network DEA approach. This study also considers different alpha values as basis for comparison to confirm whether the results obtained differ by uncertainty level. [ABSTRACT FROM AUTHOR]

TRIM28/KAP1/TIF1β is a crucial epigenetic modifier. Genetic ablation of trim28 is embryonic lethal, although RNAi-mediated knockdown in somatic cells yields viable cells. Reduction in TRIM28 abundance at the cellular or organismal level results in polyphenism. Posttranslational modifications such as phosphorylation and sumoylation have been shown to regulate TRIM28 activity. Moreover, several lysine residues of TRIM28 are subject to acetylation, but how acetylation of TRIM28 affects its functions remains poorly understood. Here, we report that, compared with wild-type TRIM28, the acetylation-mimic mutant TRIM28-K304Q has an altered interaction with Krüppel-associated box zinc-finger proteins (KRAB-ZNFs). The TRIM28-K304Q knock-in cells were created in K562 erythroleukemia cells by CRISPR-Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein nuclease 9) gene editing method. Transcriptome analysis revealed that TRIM28-K304Q and TRIM28 knockout K562 cells had similar global gene expression profiles, yet the profiles differed considerably from wild-type K562 cells. The expression levels of embryonic-related globin gene and a platelet cell marker integrin-beta 3 were increased in TRIM28-K304Q mutant cells, indicating the induction of differentiation. In addition to the differentiation-related genes, many zinc-finger-proteins genes and imprinting genes were activated in TRIM28-K304Q cells; they were inhibited by wild-type TRIM28 via binding with KRAB-ZNFs. These results suggest that acetylation/deacetylation of K304 in TRIM28 constitutes a switch for regulating its interaction with KRAB-ZNFs and alters the gene regulation as demonstrated by the acetylation mimic TRIM28-K304Q. [ABSTRACT FROM AUTHOR]

Learning style is traditionally assumed to be a predictor of learning performance, yet few studies have identified the mediating and moderating effects between the two. This study extends previous research by proposing and testing a model that examines the mediating processes in the relationship between learning style and e-learning performance and the moderating effects of prior knowledge. The results show that the sensory/intuitive dimension of learning style predicts learning performance indirectly through the mediation of online participation. However, other types of learning styles do not affect online participation. Sensory students demonstrate a higher level and intuitive students a lower level of online participation. Prior knowledge plays an important role as a moderator between online participation and learning performance. This study was conducted in the context of software usage instruction using empirical data from 219 undergraduate students. (Contains 6 tables and 3 figures.)