1. Two-signal electrochemical method for evaluation suppression and proliferation of MCF-7 cells based on intracellular purine
- Author
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Lin Runxian, Guanggang Gao, Jiguang Liu, Qian Wang, Dongmei Wu, Jiwen Cui, and Jinlian Li
- Subjects
Purine ,Guanine ,Intracellular Space ,Biophysics ,Genistein ,Antineoplastic Agents ,Biology ,Biochemistry ,chemistry.chemical_compound ,Electrochemistry ,Humans ,Purine metabolism ,Cytotoxicity ,Electrodes ,Molecular Biology ,Cell Proliferation ,Dose-Response Relationship, Drug ,Nanotubes, Carbon ,Cell Biology ,Xanthine ,Molecular biology ,chemistry ,MCF-7 ,Purines ,MCF-7 Cells ,Drug Screening Assays, Antitumor ,Intracellular - Abstract
Two electrochemical signals ascribed to xanthine/guanine and hypanthine/adenine in MCF-7 cells were detected at 0.726 and 1.053 V, respectively. Based on the intensity of signals, the genistein-induced proliferation and suppression of MCF-7 cells could be evaluated. The results showed that with the increase of genistein dose at the range of 10(-9) to 10(-6)M, the two electrochemical signals of MCF-7 cell suspension increased due to the proliferation, whereas the tendency at the high dosage range of more than 10(-5)M was decreased. The proliferation and cytotoxicity obtained by the electrochemical method were in agreement with those obtained by cell counting and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] method. Thus, the two-signal electrochemical method is an effective way to evaluate the effect of drugs on cell activity based on purine metabolism.
- Published
- 2014