The aim of the experiment was to investigate the effects of mulberry leaves and other compound powders on the growth performance, digestive enzyme activity, intestinal flora, and serum immune indexes of Lingshan Ma chickens. A total of 200 healthy 56-day-old Lingshan Ma chickens with similar body weight were randomly divided into four groups with five replicates per group and 10 chickens per replicate. The chickens in the control group were fed a basal diet, and the chickens in the group 1, group 2, and group 3 were fed a basal diet supplemented with 2.0%, 1.0%, and 0.5% mulberry leaves and other compound powders, respectively. The experiment lasted for 42 days. The results showed that compared with the control group, the average daily gain in the group 2 increased by 12.65% (P<0.05), and the feed to gain ratio significantly decreased (P<0.05). The number and diarrhea rate of Escherichia coli and Salmonella in the experimental groups significantly decreased (P<0.05), the activity of duodenal trypsin, amylase, lipase, and cecal cellulase significantly increased (P<0.05), and the serum IgG content increased by 40.69%, 49.88%, and 25.72%, respectively (P<0.05). The concentrations of serum TP, ALB, and GLB significantly increased (P<0.05), and the A/G significantly decreased (P<0.05). The study indicates that mulberry leaves and other compound powders can increase the growth performance and digestive enzyme activity, decrease the diarrhea rate, improve the serum biochemical indexes and immune function of Lingshan Ma chickens, and the appropriate amount is 1.0%. [ABSTRACT FROM AUTHOR]