10 results on '"Lin, Qun-fang"'
Search Results
2. CTHRC1 and PD‑1/PD‑L1 expression predicts tumor recurrence in prostate cancer
- Author
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Zhou, Qing, primary, Xiong, Wei, additional, Zhou, Xing, additional, Gao, Rui‑Song, additional, Lin, Qun‑Fang, additional, Liu, Hui‑Ying, additional, Li, Juan‑Ni, additional, and Tian, Xue‑Fei, additional
- Published
- 2019
- Full Text
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3. Ultrasonic degradation of polysilane polymers
- Author
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Zhou Xiaodong, Dai Gan-ce, Ji Faxiang, and Lin Qun-fang
- Subjects
chemistry.chemical_classification ,Polymers and Plastics ,Polymer ,Condensed Matter Physics ,chemistry.chemical_compound ,Viscosity ,chemistry ,Mechanics of Materials ,Polymer chemistry ,Materials Chemistry ,Polysilane ,Molar mass distribution ,Degradation (geology) ,Ultrasonic sensor ,Irradiation ,Reduced viscosity ,Composite material - Abstract
The degradation of four polysilanes in solution irradiated by ultrasonic waves has been studied by measurements of reduced viscosity and dynamic mechanical behavior. In all cases ultrasonic degradation occurs. The ultrasonic waves cause the average molecular weight to decrease and the molecular-weight distribution to become narrower in a relatively short period. The degree and rate of degradation are related to the intensity of the ultrasonic waves. In addition, the degree of degradation decreases with increasing polymer concentration and the rate increases with increasing initial molecular weight of the polysilane. After irradiation, the molecular weights of polysilanes tended to the same value from various initial values.
- Published
- 1998
4. Combination of musk and olibanum promotes proliferation of prostate stem cells in mice: An experimental study.
- Author
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LIN Qun-fang, HUANG Pei, SHANG Xue-jun, TIAN Xue-fei, ZHANG Guo-min, GAO Rui-song, ZHOU Xing, LIU Hui-ying, and ZHOU Qing
- Published
- 2017
- Full Text
- View/download PDF
5. Mechanisms of musk and olibanum regulating inflammatory cascade reaction triggered by chemotactic and activated monocytes.
- Author
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ZHOU Qing, YANG Yang, WU Yang-peng, ZHOU Xing, HAN Ping, LIN Qun-fang, PENG Jia, GAO Rui-song, and TIAN Xue-fei
- Published
- 2015
- Full Text
- View/download PDF
6. [Epidemiological and clinicopathological characteristics of prostate cancer in Hunan].
- Author
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Zou B, Long Y, Yang ZM, Gao RS, Lin QF, Dai XJ, Yi G, Wang QZ, Zhou Q, and Zhang XB
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- Male, Humans, Middle Aged, Prostate-Specific Antigen, Prostate pathology, Neoplasm Grading, Prostatectomy methods, Carcinoma, Intraductal, Noninfiltrating surgery, Prostatic Neoplasms pathology
- Abstract
Objective: To investigate the epidemiological and clinicopathological characteristics of PCa and provide some strategies for the clinical prevention and treatment of the malignancy., Methods: This study included 1 594 cases of pathologically diagnosed PCa after radical prostatectomy in Xiangya Hospital of Central South University from January 1, 2010 to December 31, 2020. We collected the basic information about the patients, their main complaints and clinicopathological results, and analyzed the epidemiological and clinicopathological data., Results: The patients were aged from 28 to 93 years, and the number of PCa cases showed an overall upward trend from 2010 to 2020. Urinary system symptoms were most common (62.53%) as initial symptoms, followed by increased PSA (17.82%), PCa, prostate nodule, prostate mass (8.43%) and bone metastasis (2.94%) found at physical examinations, and the cases of PSA elevation among the clinic visitors increased year by year from 2010 to 2020. Gleason score 7 was found in a largest proportion of the PCa patients, and adenocarcinoma was the main pathological type (78.6%). Logistic regression analysis showed that high Gleason score, instead of age and expressions of Ki67, AR and ERG, was an independent risk factor for intraductal carcinoma., Conclusion: The incidence of PCa shows an increasing trend, and is more common in those over 50 years old. PSA screening is gradually popularized in China. Intraductal carcinoma, as a major risk factor for aggressive PCa and poor prognosis of the malignancy, is significantly correlated with high Gleason scores.
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- 2022
7. [Xiongcan Yishen Prescription up-regulates the expressions of cholesterol transport proteins, steroidogenic enzymes and SF-1 in the Leydig cells of rats with late-onset hypogonadism].
- Author
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Zhou X, Li BN, Zhou HL, He QH, Zhou Q, Xiong W, Gao RS, Lin QF, and Shang XJ
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- Animals, Biological Transport, Carrier Proteins, Leydig Cells metabolism, Male, Phosphoproteins metabolism, Rats, Rats, Sprague-Dawley, Receptors, Steroid metabolism, Testosterone, Cholesterol metabolism, Drugs, Chinese Herbal therapeutic use, Hydroxysteroid Dehydrogenases metabolism, Hypogonadism drug therapy, Leydig Cells drug effects
- Abstract
Objective: To investigate the effects of Xiongcan Yishen Prescription (XYP) on the expressions of cholesterol transport proteins, steroidogenic enzymes and steroidogenic factor-1 (SF-1) in the Leydig cells of the rats with late-onset hypogonadism (LOH)., Methods: Twenty-five 18-month-old male SD rats were randomly divided into five groups of equal number, LOH model control, testosterone propionate (TP) and low-, medium- and high-dose XYP, and another 5 two-month-old male SD rats included as normal controls. After modeling, the animals in the TP group were treated by intramuscular injection of TP at 5.21 mg/kg qd alt, those in the low-, medium- and high-dose XYP groups intragastrically with XYP at 10.4, 20.8 and 41.6 g/kg qd alt respectively, and those in the LOH model and normal control groups with saline, all for 28 successive days. Then, all the rats were sacrificed for determination of the expressions of the cholesterol transport proteins StAR and TSPO, steroidogenic enzymes CYP11A1, HSD3B7 and HSD17B4, and SF-1 in the Leydig cells by Western blot., Results: The expressions of StAR, TSPO, CYP11A1, HSD3B7, HSD17B4 and SF-1 in the Leydig cells were significantly decreased in the LOH model controls compared with those in the normal controls (P< 0.05), but remarkably increased in the low-, medium- and high-dose XYP groups in comparison with those in the LOH model control group (P< 0.05)., Conclusions: Xiongcan Yishen Prescription can up-regulate the expressions of the cholesterol transport proteins StAR and TSPO, steroidogenic enzymes CYP11A1, HSD3B7 and HSD17B4, and SF-1 in the rat Leydig cells, which might be one of the possible mechanisms of the prescription in the treatment of LOH.
- Published
- 2020
8. [Combination of musk and olibanum promotes proliferation of prostate stem cells in mice: An experimental study].
- Author
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Lin QF, Huang P, Shang XJ, Tian XF, Zhang GM, Gao RS, Zhou X, Liu HY, and Zhou Q
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- Animals, Drug Therapy, Combination, Epithelial Cells cytology, Epithelial Cells drug effects, Female, Male, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells drug effects, Mice, Mice, Inbred C57BL, Mice, SCID, Pregnancy, Random Allocation, Receptor Protein-Tyrosine Kinases, Receptors, Cholinergic, Stem Cells cytology, Cell Differentiation drug effects, Cell Proliferation drug effects, Fatty Acids, Monounsaturated pharmacology, Frankincense pharmacology, Prostate cytology, Stem Cells drug effects
- Abstract
Objective: To study the effects of muskolibanum combination on the proliferation and differentiation of prostate stem cells., Methods: We cultured prostate epithelial cells and urogenital sinus mesenchymal (UGSM) cells from 7-10 d old C57BL/6 mice and 16-18 d old pregnant C57BL/6 mice, transplanted the mixed suspension of the two types of cells under the kidney envelope of SCIDCB.17 male mice, and harvested the transplants 30 days later. We randomly divided the SCIDCB.17 mice into four groups to be treated intragastrically with musk (n = 8), olibanum (n = 8), musk+olibanum (n = 7), and normal saline (blank control, n = 8)) respectively, all for 14 days. Then we collected the kidney tissue for observation of the morphology of the glandular tubes and differentiation of different subsets of stem cells by HE staining and determination of the expressions and distribution of P63, CD133, CD117 and Sca1 by immunohistochemistry and Western blot., Results: A system was successfully established for the isolation and mixed culture of Sca1 Lin+ CD49f+ (LSC) cells of prostate stem cells and UGSM cells of the mouse embryonic prostate. Immunohistochemistry showed positive expressions of P63, CD133, Sca1, and CD117 in the prostatic acinar epithelia and proved the presence of prostatic acinar epithelial structure in the transplants. Compared with the blank control group, the expressions of CD133, Sca1 and CD117 were significantly increased in the musk, olibanum, and musk+olibanum groups (P< 0.05), higher in the musk+olibanum than in the musk or olibanum group (P< 0.05), and their protein expressions were even more elevated in the musk+olibanum group (P< 0.01), with statistically significant difference from the olibanum group (P< 0.05)., Conclusions: The combination of musk and olibanum can improve the proliferation and differentiation of prostate stem cells.
- Published
- 2017
9. [Effects of the combination of musk and olibanum on the expressions of tight junction proteins in the prostate epithelial cells of rats].
- Author
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Lin QF, Huang P, Tian XF, Shang XJ, Wu YP, Han P, Gao RS, and Zhou Q
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- Animals, Claudins metabolism, Down-Regulation, Male, Occludin metabolism, Prostatitis, Rats, Rats, Sprague-Dawley, Up-Regulation, Epithelial Cells drug effects, Fatty Acids, Monounsaturated chemistry, Frankincense chemistry, Prostate cytology, Tight Junction Proteins metabolism
- Abstract
Objective: To investigate the effects of the combination of musk and olibanum on the tight junction protein expressions in prostatic epithelial cells of normal and chronic prostatitis (CP) rats., Methods: Eighty male SD rats were randomly divided into 8 groups of equal number: normal control, normal musk, normal olibanum, normal musk + olibanum, CP model control, CP model musk, CP model olibanum, and CP model musk + olibanum. At 60 days after modeling, the rats in the control, musk, olibanum, and musk + olibanum groups were treated intragastrically with normal saline, musk (0.021 g per kg body weight per day), olibanum (1.05 g per kg body weight per day), or musk + olibanum respectively, all for 3 days. Then, all the rats were sacrificed and their prostate tissues harvested for detection of the expressions of the tight junction proteins Claudin-1, Claudin-3, Occludin, and ZO-1 in the prostatic epithelial cells by immunohistochemical staining., Results: In the CP models, only the expression of Claudin-1 was significantly increased. In the normal rats, the expression of Claudin-1 was remarkably upregulated after treated with musk (824.6 ± 393.3, P < 0.05), olibanum (982.0 ± 334.0, P < 0.05), and musk + olibanum (1088.1 ± 640.2, P < 0.01); that of Claudin-3 was elevated markedly by olibanum (1 009.5 ± 243.6, P < 0.05) and insignificantly by musk (597.5 ± 80.7), but the increasing effect of olibanum was reduced by musk + olibanum (678.4 ± 255.1). No statistically significant differences were found in the expression of Occludin among the rats treated with musk (693.0 ± 424.8), olibanum (732.1 ± 302.0), and musk + olibanum (560.2 ± 202.3), or in that of ZO-1 in the animals treated with musk (290.0 ± 166.8) and olibanum (419.7 ± 108.1), but the latter was markedly decreased in the musk + olibanum group (197.7 ± 98.2, P < 0.05). In the CP rat models, both the expressions of Claudin-1 (823.0 ± 100.1, P < 0.01) and Occludin (1160.0 ± 32.2, P < 0.05) were significantly increased. The expression of Claudin-1 was remarkably down-regulated by musk (764.9 ± 179.0), olibanum (468.4 ± 220.4), and musk + olibanum (335.1 ± 204.0) (all P < 0.05), but that of Claudin-3 up-regulated by musk (744.6 ± 94.5) and olibanum (700.1 ± 223.7) (both P < 0.05). The expression of Occludin was reduced by musk (615.0 ± 221.0), olibanum (749.6 ± 321.7), and musk + olibanum (505.8 ± 523.7), while that of ZO-1 increased by olibaum (443.2 ± 44.9) and decreased by musk + olibanum (213.5 ± 24.9, P < 0.05)., Conclusion: In physiological and pathological conditions, the combination of musk and olibanum acts on the expressions of tight junction proteins in prostate epithelial cells in a selective and dual-targeting manner, promoting their permeability by down-regulating the expression of ZO-1 and maintaining their structural stability by regulating the expressions of Claudin-1, Claudin-3, and Occludin.
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- 2015
10. [Mechanisms of musk and olibanum regulating inflammatory cascade reaction triggered by chemotactic and activated monocytes].
- Author
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Zhou Q, Yang Y, Wu YP, Zhou X, Han P, Lin QF, Peng J, Gao RS, and Tian XF
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- Animals, Blotting, Western, Endothelial Cells metabolism, Inflammation metabolism, Interleukin-10 metabolism, Interleukin-1beta metabolism, Interleukin-6 metabolism, Interleukin-8, Male, Mice, Mice, Inbred BALB C, Monocytes metabolism, Rats, Rats, Sprague-Dawley, Tumor Necrosis Factor-alpha metabolism, Vascular Cell Adhesion Molecule-1 metabolism, Cytokines metabolism, Endothelial Cells drug effects, Fatty Acids, Monounsaturated pharmacology, Frankincense pharmacology, Monocytes drug effects, Prostate cytology
- Abstract
Objective: To study the effects of drug plasma of musk and olibanum (DP-M&O) on the release of inflammatory cytokines from monocytes and the expressions of the proteins associated with inflammation of prostatic or endothelial cells induced by prostate antigen (PAg) stimulation., Methods: We prepared DP-M&O using SD rats and monocytes and PAgs using BALB/c mice. We pre-treated the monocytes with DP-M&O at the gradient concentrations of 0, 2.5, 5, 10, and 20% for 1 hour, activated them with PAgs, and then cultured them for 96 hours, followed by detection of the release of inflammatory cytokines. We co-cultured the prostate RWPE-1 cells with the endothelial EA. hy926 cells, pre-treated them with the same gradient concentrations of DP-M&O as above for 1 hour, activated with PAgs, and cultured for 96 hours. Then we determined the expression levels of the proteins associated with inflammation of RWPE-1 and EA. hy926 cells by Western blot., Results: DP-M&O decreased the levels of TNF-alpha, IL-1beta, IL-6, and IL-8 and increased that of IL-10 in a concentration-dependent manner. Significant differences were found between the 20% P-M&O and PAg groups in the release of the inflammatory cytokines TNF-alpha (70.8 +/- 22.3 vs. 277.1 +/- 65.5, P < 0.01) , IL-113 (277.5 +/- 22.6 vs. 630.4 +/- 89.7, P <0.01), IL-6 (232.7 +/- 62.7 vs. 994.2 vs. 182.3, P < 0.01), IL-8 (227.3 +/- 79.2 vs. 769.3 +/- 284.1, P < 0.01), and IL-10 (640.2 +/- 201.2 vs. 271.1 +/- 55.8, P < 0.01). Compared with the PAg group, the 10 and 20% P-M&O groups showed remarkable decreases in the protein expression of MCP-1/CCL2 in the RWPE-1 cells (1.12 +/- 0.34 vs. 0.56 +/- 0.11 and 0.34 +/- 0.08) and that of VCAM-1 in the EA. hy926 cells (0.94 +/- 0.22 vs. 0.52 +/- 0.17 and 0.38 +/- 0.12) (P < 0.05 or 0.01)., Conclusion: The compatibility of musk and olibanum can decrease the expression of MCP-1/CCL2 in prostate cells and VCAM-1 in vascular endothelial cells, blocking the adhesion of leucocytes and suppressing inflammatory response.
- Published
- 2015
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