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Abstract This study aimed to investigate the relationship between hemodialysis duration (HDD) and retinal nerve fiber layer (RNFL) thickness. A total of 60 patients receiving maintenance hemodialysis and 67 healthy controls were analyzed. Spectral domain optical coherence tomography (SD-OCT) was employed to measure annular RNFL thicknesses. The hemodialysis group exhibited reduced temporal and inferior RNFL thicknesses relative to the control group. In hemodialysis patients, the inferior RNFL thickness was negatively correlated with HDD and positively correlated with intraocular pressure (IOP). Moreover, IOP was positively correlated with HDD. Mediation analysis showed that the negative correlation between HDD and inferior RNFL thickness was mediated by IOP. In conclusion, hemodialysis leads to temporal and inferior RNFL thinning, and the thickness reduction is proportional to hemodialysis duration. However, such changes are not induced by an increase in IOP.

Studies on leisure walking among older adults have received extensive attention from various areas such as public health, medicine/prevention, sports, geography, architecture, urban/transportation planning, and leisure, as they focus on various themes, including the health benefits of walking, walking behavior surveys, walking behavior, and influencing factors. In such an information-dense study, the duration and frequency of walking are proven to be critical indicators used to evaluate physical activity and are widely applied to provide an essential basis for health interventions for older adults. Leisure walking is the most common activity among older adults in China and is generally performed daily. However, studies on leisure walking among older adults in China remain lacking, leading to the urgent need for additional empirical studies. This study attempted to build a framework for the factors influencing the duration-frequency pattern of daily leisure walking among older adults using the ecological model theory, which could be used as instructions for the case studies. This study was performed using 1,285 samples of leisure walking behavior of older adults in Nanjing. Multivariate logistic regression and other methods were used to explore the duration and frequency pattern of daily leisure walking and its influencing factors among older adults in Nanjing. The results showed that: 1) even though most older adults in Nanjing have not reached the physical activity level recommended by the World Health Organization. In general, the duration and frequency of daily leisure walking among older adults in Nanjing is characterized by "medium duration and high frequency", with the medium and high duration accounting for 75.02% and the medium and high frequency accounting for 89.96%, and the duration and frequency pattern of leisure walking differed among groups. 2) Certain factors commonly influence the duration and frequency pattern of daily leisure walking among older adults in Nanjing, including individual social factors, such as gender, family structure, and residence time in Nanjing, and psychological factors, such as walking preference, walking motivation, perception of personal constraints, and walking behavior intention. 3) The study reports differential factors, including health conditions, negotiation efficacy, and perceived constraints of the built environment, that could affect the duration pattern of daily leisure walking for older adults. The study reports that age and monthly personal income were the main factors influencing the variable frequency pattern of daily leisure walking among older adults. Based on the above study, theoretical and practical implications for facilitating leisure walking participation among older adults in China are discussed.

IntroductionInflammatory and thrombotic biomarkers are simple prognostic indicators of adverse clinical outcomes in patients with ischemic stroke (IS). However, isolated assessment of inflammatory or thrombus biomarkers in patients with IS is limited in clinical practice.MethodsThis study aimed to evaluate the predictive value of a novel, simplified thrombo-inflammatory prognostic score (TIPS) that combines both inflammatory and thrombus biomarkers in the early phase of IS and to identify high-risk patients at the time of admission. The study population comprised 915 patients with a primary diagnosis of IS in the emergency departments of five grade A tertiary hospitals in China.ResultsPatients were divided into two groups based on the modified Rankin Scale (mRS):

Aims: Retinal ischemia/reperfusion (I/R) injury is implicated in the etiology of various ocular disorders. Prior research has demonstrated that bone marrow tyrosine kinase on chromosome X (BMX) contributes to the advancement of ischemic disease and inflammatory reactions. Consequently, the current investigation aims to evaluate BMX's impact on retinal I/R injury and clarify its implied mechanism of action. Main methods: This study utilized male and female systemic BMX knockout (BMX−/−) mice to conduct experiments. The utilization of Western blot assay and immunofluorescence labeling techniques was employed to investigate variations in the expression of protein and tissue localization. Histomorphological changes were observed through H&E staining and SD-OCT examination. Visual function changes were assessed through electrophysiological experiments. Furthermore, apoptosis in the retina was identified using the TUNEL assay, as well as the ELISA technique, which has been utilized to determine the inflammatory factors level. Key findings: Our investigation results revealed that the knockdown of BMX did not yield a significant effect on mouse retina. In mice, BMX knockdown mitigated the negative impact of I/R injury on retinal tissue structure and visual function. BMX knockdown effectively reduced apoptosis, suppressed inflammatory responses, and decreased inflammatory factors subsequent to I/R injury. The outcomes of the current investigation revealed that BMX knockdown partially protected the retina through downregulating phosphorylation of AKT/ERK/STAT3 pathway. Significance: Our investigation showed that BMX−/− reduces AKT, ERK, and STAT3 phosphorylation, reducing apoptosis and inflammation. Thus, this strategy protected the retina from structural and functional damage after I/R injury.

ObjectiveThe aim of this study was to detect botulinum toxin and Clostridium botulinum （C.botulinum） in 30 batches of infant formula milk powder obtained from an enterprise and to analyze the whole genome of the strain of C. botulinum type B isolated from the samples.MethodsThe samples were subjected to C. botulinum isolation and botulinum toxin typing experiments according to the “National standard for food safety microbiological examination of C. botulinum and botulinum toxin” （GB 4789.12—2016）. The whole genomes of the isolated strains were sequenced and their genetic characteristics were analyzed.ResultsBotulinum toxin was not detected in any of the 30 batches of samples. After intraperitoneal injection of the enrichment solution into mice， four batches of milk powder samples induced symptoms of botulism， but C. botulinum was isolated from only one batch of milk powder samples. Genome sequencing revealed that the bacterium was C. botulinum group Ⅰ， type B， the toxin gene cluster was type Ha， and the toxin gene was subtype B2.ConclusionThe detection of C. botulinum in infant formula milk powder with a complex background of microorganisms should not be based on the isolation of bacteria as the gold standard but should be confirmed by a mouse toxicity test of the enrichment solution combined with a botulinum toxin antiserum protection test. Whole genome sequencing can accurately identify strains and analyze relevant genetic characteristics， providing reliable technical support for handling poisoning events.

For hydraulic engineering, accurate geometric modeling is crucial for design, analysis, and maintenance. In recent years, point cloud technology, with its high precision and detailed data capture capabilities, has gradually become the preferred method for geometric modeling in hydraulic engineering. However, traditional point cloud processing methods exhibit slow data processing speeds and poor mesh quality when reconstructing 3D models. Therefore, we have studied the meshing method for the 3D reconstruction of large-scale discrete point cloud data and proposed a meshing method based on the Delaunay criterion and the growth method to achieve rapid meshing of discrete point cloud data. To demonstrate the efficiency and convenience of the proposed method, point cloud reconstruction experiments are conducted on various buildings. We compare our approach with the Poisson, BPA, and ONet methods. Additionally, we apply it to the Jiangsu Gaogang Water Conservancy Hub in China. The results show that our proposed method is superior in both reconstruction quality and efficiency, providing a promising solution for the reconstruction of 3D building models from point clouds.