Search

Your search keyword '"Lin, Huixing"' showing total 476 results
Start Over Author "Lin, Huixing"
476 results on '"Lin, Huixing"'

45. Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.

Author

Guo, Mengru, Li, Yuhui, Tang, Jinsheng, Wang, Qing, Wang, Qiancheng, Zhou, Hong, Lin, Huixing, Ma, Zhe, and Fan, Hongjie

Subjects
EXTRACELLULAR matrix proteins, BACTERIAL adhesion, BACTERIAL adhesins, BACTERIAL cells, EPITHELIAL cells, PORCINE reproductive & respiratory syndrome
Abstract

Porcine circovirus type 2 (PCV2) often causes disease through coinfection with other bacterial pathogens, including Glaesserella parasuis (G. parasuis), which causes high morbidity and mortality, but the role played by PCV2 and bacterial and host factors contributing to this process have not been defined. Bacterial attachment is assumed to occur via specific receptor-ligand interactions between adhesins on the bacterial cell and host proteins adsorbed to the implant surface. Mass spectrometry (MS) analysis of PCV2-infected swine tracheal epithelial cells (STEC) revealed that the expression of Extracellular matrix protein (ECM) Fibronectin (Fn) increased significantly on the infected cells surface. Importantly, efficient G. parasuis serotype 4 (GPS4) adherence to STECs was imparted by interactions with Fn. Furthermore, abrogation of adherence was gained by genetic knockout of Fn, Fn and Integrin β1 antibody blocking. Fn is frequently exploited as a receptor for bacterial pathogens. To explore the GPS4 adhesin that interacts with Fn, recombinant Fn N-terminal type I and type II domains were incubated with GPS4, and the interacting proteins were pulled down for MS analysis. Here, we show that rare lipoprotein A (RlpA) directly interacts with host Fibronectin mediating GPS4 adhesion. Finally, we found that PCV2-induced Fibronectin expression and adherence of GPS4 were prevented significantly by TGF-β signaling pathway inhibitor SB431542. Our data suggest the RlpA-Fn interaction to be a potentially promising novel therapeutic target to combat PCV2 and GPS4 coinfection. Author summary: Porcine circovirus type 2 and Glaesserella parasuis (G. parasuis) are common upper respiratory tract pathogens in pigs, which co-infection causes high morbidity and mortality. However, the underlying mechanisms of host-pathogen interactions during PCV2 and GPS4 co-infection remain unclear. Here, we focused on the interaction of PCV2-infected cells and GPS4, which revealed that swine tracheal epithelial cells (STEC) infected with PCV2 exhibit the increased expression of Fn. Notably, PCV2 infection-induced Fn interacting with RlpA was shown to impart efficient adherence to GPS4. Fn and Integrin β1 antibody blocking and SB431542 counteracted PCV2 infection-induced GPS4 colonization. These studies reveal a novel receptor-ligand interaction that enhances colonization of the tracheal by GPS4 following PCV2 infection and highlights the importance of Fn-RlpA in host-pathogen interactions. In addition, RlpA was discovered for the first time as an adhesin of GPS4, which provided a new idea for the development of G. parasuis vaccines. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

46. Effect of Yb2O3 doping on energy storage and dielectric properties of barium titanate based ceramics.

Author

Gao, Yang, Xie, Tianyi, Wang, Huaizhi, Yang, Junjie, and Lin, Huixing

Abstract

To produce high-performance dielectric capacitors for pulsed power applications, BaTiO3@(MgO–Nb2O5)–xYb2O3 (BT@MNY-x) ceramics were prepared via solid-state reaction route. The BT@MNY ceramics retained tetragonal perovskite structure without other obvious phases. Profound structural tests by Rietveld refinement of XRD patterns verified Yb3+ substituted the Ti4+ sites. In addition, after Yb3+ ions were incorporated into the lattice of barium titanate, the cubic phase of barium titanate was slightly distorted, the lattice constant of the sample gradually increased, and the tetragonality of the sample gradually decreased. With the increase of Yb2O3 content, the porosity and the diffuseness degree (γ) of the ceramics first decreased and then increased, reaching the peak values in the BT@MNY-0.5 sample. In addition, the grain size and dielectric constant of the samples decreased with the increase of Yb2O3 content. As a result, the breakdown voltage of the ceramics and performance metrics such as effective energy storage density first increased and then decreased. The calculated activation energies (Ea = 0.9–1.1 eV) closely resemble those observed for oxygen vacancy conduction activation energy in perovskite systems (around 1 eV), indicating that the conductivity in the BT@MNY ceramics is likely governed by oxygen vacancies. When x = 0.5, environmentally friendly barium titanate-based ceramics with Wrec of 0.82 J/cm3 and η of 44.34% were achieved. This work provides a theoretical basis for high energy storage barium titanate ceramics. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results