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30 results on '"Lin, Dongguo"'

1. Selective expansion of renal cancer stem cells using microfluidic single-cell culture arrays for anticancer drug testing.

Author

Wang, Xiaogang, He, Tao, Chen, Zihe, Chen, Jueming, Luo, Yanzhang, Lin, Dongguo, Li, Xiancheng, and Liu, Dayu

Subjects
RENAL cancer, CANCER cell culture, CELL culture, CANCER stem cells, DRUG use testing, ANTINEOPLASTIC agents, PROTEIN-tyrosine kinase inhibitors, PROTEIN-tyrosine kinases
Abstract

The suboptimal prognosis associated with drug therapy for renal cancer can be attributed to the presence of stem-cell-like renal cancer cells. However, the limited number of these cells prevents conventional drug screening assays from effectively assessing the response of renal cancer stem cells to anti-cancer agents. To address this issue, the present study employed microfluidic single-cell culture arrays to expand renal cancer stem cells by exploiting the anti-apoptosis and self-renewal properties of tumor stem cells. A microfluidic chip with 18 000 hydrophilic microwells was designed and fabricated to establish the single-cell culture array. Over a 7 day culture, the large-scale single-cell culture yielded a limited quantity of single-cell-derived tumorspheres. The sphere formation rates for Caki-1, 786-O, and ACHN cells were determined to be 8.74 ± 0.53%, 12.02 ± 1.43%, and 4.98 ± 1.68%, respectively. The expanded cells exhibited stemness characteristics, as indicated by immunofluorescence, flow cytometry, serial passaging, and in vitro differentiation assays. Additionally, the comparative transcriptomic analysis showed significant differences in the gene expression patterns of the expanded cells compared to the differentiated renal cancer cells. The drug testing indicated that renal cancer stem cells exhibited reduced sensitivity towards the tyrosine kinase inhibitors sorafenib and sunitinib, compared to differentiated renal cancer cells. This reduced sensitivity can be attributed to the elevated expression levels of tyrosine kinase in renal cancer stem cells. This present study provides evidence that the utilization of microfluidic single-cell culture arrays for selective cell expansion can facilitate drug testing of renal cancer stem cells. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis

Author

Chen, Zihe, Chen, Jueming, Lin, Dongguo, Kang, Hui, Luo, Yanzhang, Wang, Xiaogang, Wang, Lihui, and Liu, Dayu

Abstract

Single-cell-derived tumor organoids (STOs) possess a distinct genetic background, making them valuable tools for demonstrating tumor heterogeneity. In order to fulfill the high throughput demands of STO assays, we have developed a microfluidic chip containing 30 000 microwells, which is dedicated to a single cell culture approach for selective expansion and differential induction of cancer stem cells. The microwells are coated with a hydrophilic copolymer to eliminate cell adhesion, and the cell culture is supported by poly(ethylene glycol) (PEG) to establish a nonadhesive culture environment. By utilizing an input cell density of 7 × 103·mL–1, it is possible to construct a 4000 single cell culture system through stochastic cell occupation. We demonstrate that the addition of 15% PEG10000 in the cell culture medium effectively prevents cell loss while facilitating tumor stem cell expansion. As were demonstrated by HCT116, HT29, and SW480 colon cancer cells, the microfluidic approach achieved a STO formation rate of ∼20%, resulting in over 800 STOs generated from a single culture. Comprehensive analysis through histomorphology, immunohistochemistry, drug response evaluation, assessment of cell invasion, and biomarker detection reveals the heterogeneity among individual STOs. Specifically, the smaller STOs exhibited higher invasion and drug resistance capabilities compared with the larger ones. The developed microfluidic approach effectively facilitates STO formation and offers promising prospects for investigating tumor heterogeneity, as well as conducting personalized therapy-focused drug screening.

Published
2024
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26. Investigation of stainless steel 316L/zirconia joint part fabricated by powder injection molding.

Author

Gal, Chang Woo, Han, Sang Soo, Han, Jun Sae, Lin, Dongguo, and Park, Seong Jin

Subjects
STAINLESS steel, POWDER injection molding, ZIRCONIUM oxide, MICROSTRUCTURE, HYDROGEN bonding
Abstract

The joint part of stainless steel 316L (SUS316L)/zirconia was investigated. The specimens were fabricated using the powder injection molding (PIM) with insert‐injection molding. SUS316L, zirconia, and a mixture of SUS316L and zirconia were used as bonding materials. The processing conditions for PIM were developed and optimized to avoid defects in the final sintered part. The bonded parts of SUS316L/mixed layer and zirconia/mixed layer were fabricated without joint failure. However, warpage and contamination existed due to the different sintering behavior and remaining binders. To reduce the warpage, the solids loading of SUS316L were changed to 44 and 40 vol. %. The warpage was decreased by reducing the shrinkage difference between the SUS316L and mixed layer. The contamination was also reduced by increasing the powder bedding in the debinding and sintering process. The microstructures and chemical compositions were confirmed by a scanning electron microscope (SEM) and an energy dispersive X‐ray spectroscope (EDS). Migration of the main components was observed at the interface and the main components were presented together at the whole part interface and interface between 2 materials in the mixed layer. It was confirmed that the joining was done with bonding of the same materials in each layer and diffusion bonding. [ABSTRACT FROM AUTHOR]

Published
2019
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