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Your search keyword '"Lin, Dong-Feng"' showing total 10 results
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10 results on '"Lin, Dong-Feng"'

2. Development and analytical validation of a novel nasopharynx swab-based Epstein-Barr virus C promoter methylation quantitative assay for nasopharyngeal carcinoma detection.

Author

Wu, Zhi-Cong, Lin, Ke-Na, Li, Xue-Qi, Ye, Xin, Chen, Hua, Tao, Jun, Zhou, Hang-Ning, Chen, Wen-Jie, Lin, Dong-Feng, Xie, Shang-Hang, and Cao, Su-Mei

Subjects
EPSTEIN-Barr virus, NASOPHARYNX cancer, METHYLATION, NASOPHARYNX, LATENT infection, EPITHELIAL cells
Abstract

Epstein-Barr virus (EBV) C promoter (Cp) hypermethylation, a crucial factor for EBV latent infection of nasopharyngeal epithelial cells, has been recognized as a promising biomarker for nasopharyngeal carcinoma (NPC) detection. In this study, we develop a novel EBV Cp methylation quantification (E-CpMQ) assay and evaluate its diagnostic performance for NPC detection. A novel qPCR assay for simultaneous quantification of methylated- and unmethylated EBV Cp was developed by the combinational modification of MethyLight and QASM, with an innovative calibrator to improve the detection accuracy and consistency. The NP swab samples and synthetic standards were used for the analytical validation of the E-CpMQ. The diagnostic efficacy of the developed E-CpMQ assay was validated in 137 NPC patients and 137 non-NPC controls. The E-CpMQ assay can detect the EBV Cp methylation ratio in one reaction system under 10 copies with 100 % recognition specificity, which is highly correlated to pyrosequencing with a correlation coefficient over 0.99. The calibrated E-CpMQ assay reduces the coefficient of variation by an average of 55.5 % with a total variance of less than 0.06 units standard deviation (SD). Linear methylation ratio detection range from 4.76 to 99.01 %. The sensitivity and specificity of the E-CpMQ respectively are 96.4 % (95 % CI: 91.7–98.8 %), 89.8 % (95 % CI: 83.5–94.3 %). The developed E-CpMQ assay with a calibrator enables accurate and reproducible EBV Cp methylation ratio quantification and offers a sensitive, specific, cost-effective method for NPC early detection. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Development and analytical validation of a novel nasopharynx swab-based Epstein-Barr virus C promoter methylation quantitative assay for nasopharyngeal carcinoma detection

Author

Wu, Zhi-Cong, primary, Lin, Ke-Na, additional, Li, Xue-Qi, additional, Ye, Xin, additional, Chen, Hua, additional, Tao, Jun, additional, Zhou, Hang-Ning, additional, Chen, Wen-Jie, additional, Lin, Dong-Feng, additional, Xie, Shang-Hang, additional, and Cao, Su-Mei, additional

Published
2023
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4. Utility of Epstein–Barr Virus DNA in Nasopharynx Swabs as a Reflex Test to Triage Seropositive Individuals in Nasopharyngeal Carcinoma Screening Programs

Author

Chen, Geng Hang, primary, Liu, Zhiwei, additional, Yu, Kelly J, additional, Coghill, Anna E, additional, Chen, Xiao Xia, additional, Xie, Shang Hang, additional, Lin, Dong Feng, additional, Huang, Qi Hong, additional, Lu, Yu Qiang, additional, Ling, Wei, additional, Lin, Chu Yang, additional, Lu, Zi Jian, additional, Fan, Yu Ying, additional, Tang, Lin Quan, additional, Sampson, Joshua N, additional, Li, Hui, additional, King, Ann D, additional, Middeldorp, Jaap M, additional, Hildesheim, Allan, additional, and Cao, Su Mei, additional

Published
2022
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9. Real-time quantitative optical method to study temperature dependence of crack propagation process in colloidal photonic crystal film

Author

Shi Jiang-Jian, Meng Qing-Bo, Luo Yan-Hong, Lin Dong-Feng, Zhang Yu, Xu Yu-Zhuan, and LI Dong-Mei

Subjects
Materials science, General Physics and Astronomy, Fracture mechanics, Physics::Classical Physics, Physics::Geophysics, Condensed Matter::Materials Science, symbols.namesake, Reaction rate constant, Scientific method, symbols, Negative temperature, Composite material, Thin film, Deformation (engineering), van der Waals force, Resultant force
Abstract

A real-time quantitative optical method to characterize crack propagation in colloidal photonic crystal film (CPCF) is developed based on particle deformation models and previous real-time crack observations. The crack propagation process and temperature dependence of the crack propagation rate in CPCF are investigated. By this method, the crack propagation rate is found to slow down gradually to zero when cracks become more numerous and dense. Meanwhile, with the temperature increasing, the crack propagation rate constant decreases. The negative temperature dependence of the crack propagation rate is due to the increase of van der Waals attraction, which finally results in the decrease of resultant force. The findings provide new insight into the crack propagation process in CPCF.

Published
2015

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