393 results on '"Lima MM"'
Search Results
2. DETECÇÃO DE ALOANTICORPOS POR ADSORÇÃO ERITROCITÁRIA EM PACIENTES COM ANEMIA HEMOLÍTICA AUTOIMUNE
- Author
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Lima, LHP, Rodrigues, GGS, Mafra, ALA, Coury, FMA, Mühlbeier, DFM, Azevedo, NC, Coelho, LA, Silva, TF, Lima, MM, and André, FGU
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- 2024
- Full Text
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3. Acoustic spectral hole-burning in a two-level system ensemble
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Andersson, G, Bilobran, ALO, Scigliuzzo, M, de Lima, MM, Cole, JH, Delsing, P, Andersson, G, Bilobran, ALO, Scigliuzzo, M, de Lima, MM, Cole, JH, and Delsing, P
- Abstract
Microscopic two-level system (TLS) defects at dielectric surfaces and interfaces are among the dominant sources of loss in superconducting quantum circuits, and their properties have been extensively probed using superconducting resonators and qubits. We report on spectroscopy of TLSs coupling to the strain field in a surface acoustic wave (SAW) resonator. The narrow free spectral range of the resonator allows for two-tone spectroscopy where a strong pump is applied at one resonance, while a weak signal is used to probe a different mode. We map the spectral hole burnt by the pump tone as a function of frequency and extract parameters of the TLS ensemble. Our results suggest that detuned acoustic pumping can be used to enhance the coherence of superconducting devices by saturating TLSs.
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- 2021
4. Melasma and pregnancy in southern Brazil
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Hexsel, D, Rodrigues, TC, DalʼForno, T, Zechmeister-Prado, D, and Lima, MM
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- 2009
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5. Synthesis and Potential Adsorption of Fe3O4@C Core-Shell Nanoparticles for to Removal of Pollutants in Aqueous Solutions: A Brief Review
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Muller L, Riella Hg, C Soares, Macuvele Dlp, Márcio Antônio Fiori, Nones J, Lima Mm, and Lucinao Luiz Silva
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Pollutant ,Paramagnetism ,Materials science ,Adsorption ,Aqueous solution ,Chemical engineering ,Magnetic core ,Magnetic separation ,Nanoparticle ,Reusability - Abstract
Core-shell Fe3O4@C nanoparticles consist of a magnetic core and carbon coating, and exhibit high adsorptive capacity, easy magnetic separation and reusability. Due to these properties, core–shell Fe3O4@C nanoparticles have great potential application in adsorption, separation and wastewater treatment. Magnetic separation based on the super paramagnetic Fe3O4 has received considerable attention and is widely used for the removal of dye, oil pharmaceuticals and metals from water due to its high efficiency and economic viability. Therefore, the main characteristics of the core-shell Fe3O4@C nanoparticles, the different types of synthesis, as well as the main applications for the removal of water pollutants were reviewed. In this brief review, an overview of the basic properties of the Fe3O4@C core-shell nanoparticles are given, and the most important and more frequently used methods for the synthesis of Fe3O4@C core-shell nanoparticles have been summarized along with the description of its adsorbent application potential.
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- 2017
6. Effectiveness of Electronic Apex Locator during the Root Canal Length in Primary Teeth: An In Vitro Study
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Sousa, DL, primary, Evangelista, DM, additional, Lima, MM, additional, Costa, ISA, additional, Sousa, RBRA, additional, and Moreira Neto, JJS, additional
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- 2015
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7. Revealing the diversity of Cloeodes Traver, 1938 (Ephemeroptera: Baetidae) in the Neotropics: description of eleven new species from Brazilian mountain ranges
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Jean-Luc Gattolliat, Lima Mm, Frederico Falcão Salles, Fabiana Criste Massariol, Michel Sartori, and Kamila Batista Angeli
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Baetidae ,Mayfly ,Cloeodes ,biology ,Ecology ,Tergum ,Animal Science and Zoology ,Taxonomy (biology) ,Body size ,biology.organism_classification ,Nymph ,Ecology, Evolution, Behavior and Systematics - Abstract
In the present work, based on material from distinct mountain ranges in Brazil, 11 new species of Cloeodes with hind wings or hind wing pads are described, illustrated and discussed. Among the new species, in C. aiuruoca, C. amantykyra, C. atlanticus, C. boldrinii and C. ioachimi, the apex of the fore femora in the nymphs is extremely projected (a characteristic previously found only in two species of the genus). Cloeodes guara and C. tracheatus share the presence of unusual large and dark gills. Cloeodes melanotarsus is readily distinguished by conspicuous blackish tarsi, C. lucifer by the bright yellow coloration of tergum I, and C. xyrognathos by blade-like incisors. Cloeodes magnus, besides being the largest species of the genus, with a body size reaching 12 mm, possess short maxillary palp. Comments on the presence of C. irvingi and C. opacus in Brazil are also provided. An interactive online key is provided for the nymphs of all the species of Cloeodes in which hind wing pads are present.
- Published
- 2015
8. Incidence of two cases of polyuria-polydipsia in confined horses
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Neto, Jcl, Carvalho, Mb, Martins, Lp, Marques, Lc, Fagliari, Jj, Trinque, Cln, Lima, Mm, and Universidade Estadual Paulista (Unesp)
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psychogenic polydipsia ,poliuria-polydipsia ,horse - Abstract
Made available in DSpace on 2020-12-10T16:29:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-06-01 Two four-year-old male Brasileiro de Hipismo breed horses presenting signs of polyuria-polydipsia were submitted to a complete clinical evaluation as well as having blood and urine samples collected for laboratory analysis. No clinical, hematological or biochemical abnormalities were observed, but a significant decrease in urine specific gravity was detected in both horses. One of the horses presented 1,009 and the other 1,008. A 24-hour water deprivation test was carried out and, during this period, the urine specific gravity gradually increased reaching the normal values of 1,028 and 1,026. The abnormal behavior occured as a consequence of an excessive confinement. The clinical and laboratory results associated to the 24-hour water deprivation test indicated a diagnosis of psychogenic polydipsia. UNESP, Fac Ciencias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, Fac Ciencias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil
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- 2000
9. Mining of Sugarcane ESTs involved in the mechanisms against plant pathogen and environmental stress
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Kuramae, E.E., Fenille RC, Rosa Jr. VE, Dias DR, Monteiro JP, Gimenes MA, Palmieri DA, Lima MM, Fávaro RD, Kuramae, E.E., and Fenille RC, Rosa Jr. VE, Dias DR, Monteiro JP, Gimenes MA, Palmieri DA, Lima MM, Fávaro RD
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- 2002
10. Quality of sleep and quality of life in people with type 2 diabetes.
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Vieira V, Verussa T, Lagacci M, Ueno M, Cocetti M, Ceolim M, and Lima MM
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- 2008
11. LATENT TUBERCULOSIS IN PATIENTS WITH CROHN'S DISEASE IN A UNIVERSITY HOSPITAL IN NORTHEASTERN BRAZIL: A RETROSPECTIVE STUDY.
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Melo IV, Santos MO, Sousa KAA, Abi-Chacra EA, Araújo TME, Lima MM, Parente JML, and Campelo V
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- Humans, Male, Retrospective Studies, Female, Adult, Cross-Sectional Studies, Brazil epidemiology, Middle Aged, Young Adult, Adolescent, Rifampin therapeutic use, Aged, Isoniazid therapeutic use, Antitubercular Agents therapeutic use, Crohn Disease complications, Latent Tuberculosis epidemiology, Latent Tuberculosis complications, Latent Tuberculosis diagnosis, Hospitals, University statistics & numerical data
- Abstract
Background: Among chronic condition problems, tuberculosis still represents a serious public health problem globally., Objective: To investigate latent tuberculosis infection in patients with Crohn's disease. Retrospective, descriptive cross-sectional study of quantitative analysis., Methods: The research was conducted on diagnosed cases of Crohn's disease at the University Hospital located in a city in Northeastern Brazil. All cases of patients with Crohn's disease undergoing isoniazid or rifampicin therapy for latent tuberculosis (LTBI) were included in the study. The data obtained were subsequently subjected to statistical analysis using the Statistical Package for the Social Sciences (SPSS) program., Results: We analyzed 235 medical records, and it was observed that 56% were male, with a mean age of 42.7. Among these, 54% declared themselves as brown, 31% had completed high school, and 47% were residents of the city of Teresina. Regarding the clinical and epidemiological characteristics of the studied patients classified as having ILTB, 34% of the medical records were diagnosed by tuberculin test, 48.51% were investigated by x-ray examination, and the recent location affected the colon with 27%., Conclusion: Overall, the health profile of the participants in this study aligns with findings previously established in the literature, particularly studies conducted in other Brazilian states, as well as in other developing countries.
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- 2024
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12. Quercetin and ibuprofen combination displayed anti-inflammatory effects and also extenuates the enteric neurons damage of arthritic rats.
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Silva BTD, Martins-Perles JVC, Bossolani GDP, Lima MM, Sehaber-Sierakowski CC, Gremaschi LB, Cunha JPSE, Bersani-Amado CA, and Zanoni JN
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- Animals, Rats, Male, Neurons drug effects, Neurons pathology, Arthritis, Experimental drug therapy, Arthritis, Experimental pathology, Enteric Nervous System drug effects, Enteric Nervous System pathology, Immunohistochemistry, Ileum drug effects, Ileum pathology, Quercetin pharmacology, Quercetin therapeutic use, Ibuprofen pharmacology, Ibuprofen therapeutic use, Rats, Wistar, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents therapeutic use, Antioxidants pharmacology, Antioxidants therapeutic use
- Abstract
This study aimed to investigate the antioxidant and anti-inflammatory properties of quercetin on the cellular components of the Enteric Nervous System in the ileum of rats with arthritis. Rats were distributed into five groups: control (C), arthritic (AIA), arthritic treated with ibuprofen (AI), arthritic treated with quercetin (AQ) and arthritic treated with both ibuprofen and quercetin (AIQ). The ileum was processed for immunohistochemical techniques for HuC/D, calcitonin gene-related peptide, and vasoactive intestinal polypeptide. Measurements in histological sections, chemiluminescence assays, and total antioxidant capacity were also performed. Rheumatoid arthritis resulted in a decrease in neuronal density, yet neuroplasticity mechanisms were evident through observed changes in varicosities size and neuronal area compared to the control group. Reduced paw edema and neuroprotective effects were predominantly noted in both plexuses, as evidenced by the increased density preservation of HuC/D-IR neurons in the AIQ group. The increase of lipoperoxidation levels and paw edema volume in the AQ group was observed compared to the arthritic, whereas the AIQ group mainly showed similar results to those observed in the control. The enteropathy associated with arthritis proved to be significant in the field of gastroenterology, and the combination of quercetin and ibuprofen demonstrated promising anti-inflammatory and neuroprotective effects.
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- 2024
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13. Dereplication of 4-Quinolone Alkaloids from Waltheria Indica (Malvaceae) Tissues Using Molecular Network Tools.
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de Medeiros Silva R, de Castro Lima MM, and Cotinguiba F
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- 4-Quinolones chemistry, 4-Quinolones pharmacology, 4-Quinolones isolation & purification, Chromatography, High Pressure Liquid, Plant Leaves chemistry, Plant Roots chemistry, Molecular Structure, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Extracts isolation & purification, Tandem Mass Spectrometry, Alkaloids chemistry, Alkaloids isolation & purification, Alkaloids pharmacology
- Abstract
Waltheria indica (Malvaceae) is a plant popularly used in folk medicine by traditional African and indigenous communities, and in various countries worldwide, to treat general inflammation. Several biological activities of this plant have been reported, including acetylcholinesterase inhibition and potential anti-human immunodeficiency virus (HIV), antinociceptive, analgesic, antifungal, anticancer, anti-inflammatory, leishmanicidal, trypanocidal, antioxidant, and antibacterial activities. The chemical profile of Waltheria indica was assessed by dereplication analysis using UPLC-MS/MS, and data acquisition was performed using chemoinformatics tools, such as Mass Spectrometry-Data Independent AnaLysis (MS-DIAL) and MS-FINDER softwares. The preprocessed data were sent to the GNPS to build a feature-based molecular network (FBMN). Thirty-three 4-quinolone alkaloids were annotated in the extracts and fractions of stems and roots, whereas 12 were annotated in the extracts and fractions of flowers and leaves. This represents an inaugural chemical investigation study employing UPLC-Q-TOF-MS/MS analysis, along with a molecular network approach, within this species and genus., (© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
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- 2024
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14. Detection of encephalitis-causing viruses reveals predominance of chikungunya virus in the state of Bahia, Brazil.
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Sampaio MPS, do Rosário MS, Martins LC, Trindade LVL, Francisco MVLO, Costa BGG, Vasconcelos GA, Lima IAB, Macêdo YSF, Carvalho FML, de Santana MBR, Khouri R, Fritsch H, Xavier J, Fonseca V, Giovanetti M, de Mello ALES, Pereira FM, Campos GS, de Jesus PAP, Farias DS, de Souza MS, Galvão AJP, Costa FO, Bessa MC, Chagas JRLP, Silvany C, Teles JMM, de Lima MM, Farias TLA, Gräf T, and de Siqueira IC
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- Humans, Brazil epidemiology, Male, Female, Adult, Adolescent, Child, Young Adult, Middle Aged, Child, Preschool, Antibodies, Viral blood, Encephalitis, Viral epidemiology, Encephalitis, Viral virology, Encephalitis, Viral diagnosis, Immunoglobulin M blood, Aged, Dengue Virus genetics, Dengue Virus isolation & purification, Infant, Phylogeny, Herpesvirus 3, Human genetics, Herpesvirus 3, Human isolation & purification, Enterovirus isolation & purification, Enterovirus genetics, Whole Genome Sequencing, Chikungunya virus genetics, Chikungunya virus isolation & purification, Chikungunya Fever epidemiology, Chikungunya Fever virology, Chikungunya Fever diagnosis, Chikungunya Fever blood
- Abstract
Objectives: Encephalitis is a severe neurological syndrome for which herpesvirus and enteroviruses are the most common etiological agents. Arboviruses, a wildly diverse group of pathogens, are also critical epidemiological agents associated with encephalitis. In Brazil, little is known about the causative agents of encephalitis., Methods: We conducted a hospital surveillance for encephalitis between 2020 and 2022. Molecular (RT-PCR and qPCR) and serological (virus-specific IgM and viral antigens) techniques were performed in cerebrospinal fluid and serum samples obtained from study participants., Results: In the 43 participants evaluated, the etiologic agent or the presence of IgM was detected in 16 (37.2%). Nine (20.9%) cases were positive for chikungunya virus (CHIKV), three (7.0%) for dengue virus, two (4.7%) for human adenovirus, one (2.3%) for varicella-zoster virus, and one (2.3%) for enterovirus. Whole-genome sequencing revealed that the CHIKV identified belongs to the East/Central/South African lineage., Conclusion: Herein, CHIKV is a common pathogen identified in encephalitis cases. Our results reinforce previous evidence that chikungunya represents a significant cause of encephalitis during CHIKV outbreaks and epidemics and add to existing information on the epidemiology of encephalitis in Brazil., Competing Interests: Declarations of competing interest The authors declare no conflicts of interest., (Copyright © 2024. Published by Elsevier Ltd.)
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- 2024
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15. Adaptation strategies for preparing for childbirth in the context of the pandemic: Roy's Theory.
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Pickler L, Lima MM, Roque ATF, Wilhelm LA, Curcio F, Guarda D, Costa R, and Alves IFBO
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- Humans, Female, Pregnancy, Adult, Pregnant Women psychology, Brazil, COVID-19 epidemiology, COVID-19 psychology, Qualitative Research, Parturition psychology, Pandemics, Adaptation, Psychological, SARS-CoV-2
- Abstract
Objectives: to understand the process of adapting to childbirth during the COVID-19 pandemic from the perspective of a group of pregnant women., Methods: a qualitative, descriptive-exploratory study was conducted with 23 women. Data were collected between October and December 2021 through documentation and semi-structured interviews, which were analyzed using Minayo's methodology and Roy's Adaptation Model., Results: various types of stimuli - focal, contextual, and residual - were identified as influencing childbirth preparation. The online group was essential for facilitating pregnant women's adaptation, offering significant support and generating positive feedback for childbirth preparation., Final Considerations: the importance of pregnant women's groups as a strategy for improving adaptation to childbirth was identified, underscoring the effectiveness of this support among professionals and participants, as well as among pregnant women. This support network strengthened preparation for childbirth during a challenging period like the pandemic.
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- 2024
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16. Prognostic implications of the ID1 expression in acute myeloid leukemia patients treated in a resource-constrained setting.
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Lima AS, Bezerra MF, Moreira-Aguiar A, Weinhäuser I, Santos BL, Falcão RM, Salustiano-Bandeira ML, Franca-Neto PL, Lima MM, Saldanha-Araujo F, Coelho-Silva JL, Pereira-Martins DA, Bezerra MA, and Lucena-Araujo AR
- Abstract
Introduction: The aberrant expression of the inhibitor of DNA binding (ID1) gene has been frequently associated with the leukemogenesis and prognostication acute myeloid leukemia (AML), although its clinical importance has never been investigated in patients treated outside well-controlled clinical trials., Methods: Using quantitative real-time polymerase chain reaction, we investigated the role of the ID1 expression in the clinical outcomes of non-selected patients with acute myeloid leukemia treated in a real-life setting., Results: Overall, 128 patients were enrolled. Patients with high ID1 expression had a lower 3-year overall survival (OS) rate of 9%, with the 95% confidence interval (95%CI) at 3 to 20%, compared to patients with a low ID1 expression (22%, 95%CI: 11 - 34%) (p = 0.037), although these findings did not retain significance after adjustment (hazard ratio (HR): 1.5, 95%CI: 0.98 - 2.28; p = 0.057). The ID1 expression had no impact on post-induction outcomes (disease-free survival, p = 0.648; cumulative incidence of relapse, p = 0.584)., Conclusions: Although we are aware thar our data are confronted with many variables that cannot be fully controlled, including drug unavailability, risk-adapted treatment, comorbidities and the time from diagnosis to treatment initiation, we are firm believers that such an initiative can provide more realistic data on understudied populations, in particular those from low- and middle-income countries., Competing Interests: Conflicts of interest The authors have no competing financial interests to declare., (Copyright © 2023 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier España, S.L.U. All rights reserved.)
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- 2024
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17. The risk of vector transmission of Trypanosoma cruzi remains high in the State of Paraná.
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Trovo JVS, Weber-Lima MM, Prado-Costa B, Iunklaus GF, Andrade AJ, Sobral-Souza T, Muylaert RL, Alvarenga LM, and Toledo MJO
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- Animals, Brazil epidemiology, Humans, Risk Factors, Risk Assessment, Ecosystem, Chagas Disease transmission, Insect Vectors classification, Insect Vectors parasitology, Trypanosoma cruzi, Triatominae classification, Triatominae parasitology
- Abstract
Background: Monitoring and analysing the infection rates of the vector of Trypanosoma cruzi, that causes Chagas disease, helps assess the risk of transmission., Objectives: A study was carried out on triatomine in the State of Paraná, Brazil, between 2012 and 2021 and a comparison was made with a previous study. This was done to assess the risk of disease transmission., Methods: Ecological niche models based on climate and landscape variables were developed to predict habitat suitability for the vectors as a proxy for risk of occurrence., Findings: A total of 1,750 specimens of triatomines were recorded, of which six species were identified. The overall infection rate was 22.7%. The areas with the highest risk transmission of T. cruzi are consistent with previous predictions in municipalities. New data shows that climate models are more accurate than landscape models. This is likely because climate suitability was higher in the previous period., Main Conclusion: Regardless of uneven sampling and potential biases, risk remains high due to the wide presence of infected vectors and high environmental suitability for vector species throughout the state and, therefore, improvements in public policies aimed at wide dissemination of knowledge about the disease are recommended to ensure the State remains free of Chagas disease.
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- 2024
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18. Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals.
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Oliveira JM, Silva DPD, Floresta LRS, Rocha GG, Almeida LIM, Dias EHV, Lima TK, Marinho JZ, Lima MM, Valer FB, Oliveira F, Rocha TL, Alvino V, Anhezini L, and Silva ACA
- Abstract
Doping nanoparticles represents a strategy for modulating the energy levels and surface states of nanocrystals (NCs), thereby enhancing their efficiency and mitigating toxicity. Thus, we herein focus on the successful synthesis of pure and gold (Au)-doped zinc oxide (ZnO) nanocrystals (NCs), investigating their physical-chemical properties and evaluating their applicability and toxicity through in vitro and in vivo assessments. The optical, structural, and photocatalytic characteristics of these NCs were scrutinized by using optical absorption (OA), X-ray diffraction (XRD), and methylene blue degradation, respectively. The formation and doping of the NCs were corroborated by the XRD and OA results. While the introduction of Au as a dopant did induce changes in the phase and size of ZnO, a high concentration of Au ions in ZnO led to a reduction in their photocatalytic activity. This demonstrated a restricted antibacterial efficacy against Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus . Remarkably, Au-doped counterparts exhibited enhanced biocompatibility in comparison to ZnO, as evidenced in both in vitro (murine macrophage cells) and in vivo ( Drosophila melanogaster ) studies. Furthermore, confocal microscopy images showed a high luminescence of Au-doped ZnO NCs in vivo . Thus, this study underscores the potential of Au doping of ZnO NCs as a promising technique to enhance material properties and increase biocompatibility., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)
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- 2024
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19. High-risk space-time clusters of mortality in children 5 to 14 years of age in Mato Grosso state, Brazil.
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Lima MM, Gama SGND, Favacho ARM, and Souza-Santos R
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- Brazil epidemiology, Humans, Child, Adolescent, Child, Preschool, Space-Time Clustering, Age Factors, Female, Male, Risk Factors, Multivariate Analysis, Cluster Analysis, Child Mortality trends
- Abstract
The study aimed to detect high-risk areas for deaths of children and adolescents 5 to 14 years of age in the state of Mato Grosso, Brazil, from 2009 to 2020. This was an exploratory ecological study with municipalities as the units of analysis. Considering mortality data from the Mortality Information System (SIM) and demographic data from the Brazilian Institute of Geography and Statistics (IBGE), the study used multivariate statistics to identify space-time clusters of excess mortality risk in this age group. From 5 to 9 years of age, two clusters with high mortality risk were detected; the most likely located in the state's southern mesoregion (RR: 1.6; LRT: 8,53). Among the 5 clusters detected in the 10-14-year age group, the main cluster was in the state's northern mesoregion (RR: 2,26; LRT: 7,84). A reduction in mortality rates was observed in the younger age group and an increase in these rates in the older group. The identification of these clusters, whose analysis merits replication in other parts of Brazil, is the initial stage in the investigation of possible factors associated with morbidity and mortality in this group, still insufficiently explored, and for planning adequate interventions.
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- 2024
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20. [Estimation of prevalence of chronic Chagas disease in Brazilian municipalitiesEstimación de la prevalencia de la enfermedad de Chagas crónica en los municipios brasileños].
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Laporta GZ, Lima MM, Maia da Costa V, de Lima Neto MM, Palmeira SL, Rodovalho SR, and Aragón López MA
- Abstract
Objective: The objective of this study is to estimate the prevalence of chronic Chagas disease (CCD) in Brazil: in the general population, in women, and in women of childbearing age., Methods: A meta-analysis of the literature was conducted to extract data on the prevalence of CCD in municipalities in Brazil in the 2010-2022 period: in the general population, in women, and in women of childbearing age. Municipal-level CCD indicators available in health information systems were selected. Statistical modeling of the data extracted from the meta-analysis (based on data obtained from information systems) was applied to linear, generalized linear, and additive models., Results: The five most appropriate models were selected from a total of 549 models tested to obtain a consensus model (adjusted R
2 = 54%). The most important predictor was self-reported CCD in the primary health care information system. Zero prevalence was estimated in 1 792 (32%) of Brazil's 5 570 municipalities; in the remaining 3 778 municipalities, average prevalence of the disease was estimated at 3.25% (± 2.9%). The number of carriers of CCD was estimated for the Brazilian population (~3.7 million), for women (~2.1 million) and for women of childbearing age (~590 000). The disease reproduction rate was calculated at 1.0336. All estimates refer to the 2015-2016 period., Conclusions: The estimated prevalence of CCD, especially among women of childbearing age, highlights the challenge of vertical transmission in Brazilian municipalities. Mathematical projections suggest that these estimates should be included in the national program for the elimination of vertical transmission of Chagas disease.- Published
- 2024
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21. Pseudoaneurysm in carotid artery after peripheral ECMO.
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Rodríguez Lima MM, Parrilla Parrilla J, and Guillén Rodríguez I
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- Humans, Carotid Artery, Internal, Stents, Aneurysm, False diagnostic imaging, Aneurysm, False etiology, Extracorporeal Membrane Oxygenation adverse effects
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- 2024
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22. pH-controlled breakup of fractal aggregates, microgels and gels formed by self-assembled amphiphilic triblock copolymers.
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Balakrishnan G, De Souza Lima MM, Niepceron F, Colombani O, Nicolai T, and Chassenieux C
- Abstract
The degradation of (micro)gels and fractal aggregates based on self-assembled amphiphilic triblock copolymers has been investigated in water by confocal microscopy and light scattering respectively. The triblock copolymer consisted of a central hydrophilic poly(acrylic acid) (pAA) block and two hydrophobic end blocks that contained an equal amount of randomly distributed n -butyl acrylate ( n BA) and AA units. These latter units helped at tempering the hydrophobic end blocks resulting in the control and the fine tuning of the dynamics of the self-assembled triblock through the pH. Starting from a pH where the dynamics is frozen, the rate of breakup of the macroscopic gels, microgels and of fractal aggregates was measured after increasing the pH to different values. The mechanism of the breakup was found to be independent of the pH, but its rate increased exponentially with increasing pH. The degradation proceeded through the release of the polymers from the bulk into the surrounding aqueous phase.
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- 2024
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23. Validity and reliability of the italian-Neonatal skin risk assessment scale (i-NSRAS).
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Curcio F, Vaquero Abellán M, Dioni E, de Lima MM, Ez Zinabi O, and Romero Saldaña M
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- Adult, Infant, Child, Humans, Infant, Newborn, Cross-Sectional Studies, Reproducibility of Results, Surveys and Questionnaires, Risk Assessment methods, Psychometrics, Italy, Pressure Ulcer prevention & control
- Abstract
Background: The skin of infants has important anatomical and physiological differences from that of children and adults. Because of skin immaturity, reduced mobility, neurological changes, and the need for medical devices, infants are at high risk for pressure injuries. Specific, validated tools are needed to assess the risk of pressure injuries in this population., Objectives: To assess the psychometric properties of the Italian version of Neonatal Skin Risk Assessment Scale (construct validity, internal consistency and reliability)., Method: A cross-sectional descriptive study was conducted in the neonatal units of the two Italian hospitals. 200 infants were examined 3 times by 54 nurses to assess the risk of pressure injuries. Exploratory and confirmatory factor analysis were performed jointly to assess construct validity. Internal consistency was analyzed using McDonalds omega coefficient and Cronbach's alpha, while intra and interobserver agreement using Intraclass Correlation Coefficients (ICC)., Results: Exploratory factor analysis confirmed a 2-factor model; the factor "duration and intensity of pressure" explained four subscales (mental state, mobility, activity and nutrition), while "skin immaturity" explained the subscales (general physical condition and skin moisture). Confirmatory factor analysis results indicated good model fits (X2/df = 0.84, p = 0.002, RMSEA = 0, NNFI = 1.01, NFI = 0.98 y CFI = 1 (M1 with MI). The italian-Neonatal Skin Risk Assessment Scale showed a good internal consistency, McDonalds omega coefficient and Cronbach's alpha both 0.86. An excellent intra and inter observer reliability was also observed, ICC 0.99 and 0.98 respectively., Conclusion: The psychometric characteristics indicate that the italian-Neonatal Skin Risk Assessment Scale is useful, valid and reliable for measuring risk of pressure injuries in the neonatal population., Implications for Clinical Practice: This is the first valid and reliable newborn-specific scale that assesses the presence of medical devices. Its use could enable efficient management of preventive resources, early classification of newborns at risk and assignment of preventive interventions, facilitate the development of risk assessment protocols., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2024
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24. Shifting patterns of dengue three years after Zika virus emergence in Brazil.
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Pinotti F, Giovanetti M, de Lima MM, de Cerqueira EM, Alcantara LCJ, Gupta S, Recker M, and Lourenço J
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- Humans, Brazil epidemiology, Antibodies, Viral, Cross Reactions, Zika Virus, Zika Virus Infection, Dengue Virus, Dengue
- Abstract
In 2015, the Zika virus (ZIKV) emerged in Brazil, leading to widespread outbreaks in Latin America. Following this, many countries in these regions reported a significant drop in the circulation of dengue virus (DENV), which resurged in 2018-2019. We examine age-specific incidence data to investigate changes in DENV epidemiology before and after the emergence of ZIKV. We observe that incidence of DENV was concentrated in younger individuals during resurgence compared to 2013-2015. This trend was more pronounced in Brazilian states that had experienced larger ZIKV outbreaks. Using a mathematical model, we show that ZIKV-induced cross-protection alone, often invoked to explain DENV decline across Latin America, cannot explain the observed age-shift without also assuming some form of disease enhancement. Our results suggest that a sudden accumulation of population-level immunity to ZIKV could suppress DENV and reduce the mean age of DENV incidence via both protective and disease-enhancing interactions., (© 2024. The Author(s).)
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- 2024
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25. Guidelines for Trypanosoma cruzi-HIV Co-infection and other Immunosuppressive Conditions: Diagnosis, Treatment, Monitoring, and Implementation from the International Network of Care and Studies - 2023.
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Almeida EA, Mendes FSNS, Ramos Júnior AN, Sousa AS, Pavan TBS, Mediano MFF, Ostermayer AL, Hasslocher-Moreno AM, Britto CFPC, Novaes CG, Correia D, Santos FLN, Silva GMSD, Fernandez ML, Lima MM, Carvalho NB, Moreira ODC, Albajar-Viñas P, Leite RM, Palmeira SL, Costa VMD, and Yasuda MAS
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- Humans, Trypanosoma cruzi, Coinfection, HIV Infections
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- 2023
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26. Phase angle values and ultra-processed food consumption are associated with changes in oxidative stress in inflammatory bowel disease patients.
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Severo JS, da Silva Barros VJ, Moraes Mendes PH, Dos Santos BLB, da Silva ACA, de Oliveira KBV, de Moura MSB, de Almeida Fonseca Viola PC, do Nascimento Nogueira N, Luz Parente JM, Lima MM, Dos Santos AA, and Silva MTB
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- Adult, Humans, Oxidative Stress, Antioxidants, Body Composition, Glutathione, Food, Processed, Inflammatory Bowel Diseases
- Abstract
Background & Aims: Changes in dietary habits including increased intake of refined sugars and fats and decreased intake of fiber have been suggested as potential risk factors for the development of inflammatory bowel diseases (IBD). Bioelectrical impedance analysis-derived phase angle (PhA) has been gaining attention in the clinical evaluation of nutritional status. In this study, we for the first time investigated the relationship of PhA and ultra-processed food intake with oxidative stress, body composition and biochemical parameters in adult patients with IBD., Methods: Body composition and PhA were evaluated through electrical bioimpedance. Nitrite (Nox), myeloperoxidase (MPO), glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in both groups. Food consumption was obtained by a food frequency questionnaire (FFQ)., Results: In comparison with the control group, the IBD group had increased (p < 0.05) concentrations of Nox (19.95 ± 1.4 vs. 35.43 ± 7.7 μM), MDA (0.70 ± 0.31 vs. 4.56 ± 0.62 nmol/L), and GSH (9.35 ± 0.38 vs. 10.74 ± 0.51 mg NPSH/μL plasma). PhA was positively correlated with GSH (R
2 :0.22; p:0.02) and SOD (R2 :0.25; p:0.01). IBD patients ingested higher amounts of ultra-processed foods (IBD:17.04 ± 2.76 vs. Control:24.88 ± 2.30%). However, IBD patients had better consumption of unprocessed or minimally processed foods (IBD:79.06 ± 3.07 vs. Control:67.83 ± 2.32%). We found a positive correlation between ultra-processed food consumption and MDA (R2 0.43; p:0.01)., Conclusions: PhA may be a practical and effective measure in clinical follow-up of IBD patients, being associated with bilirubin levels and antioxidant enzymes. Also, we recommend evaluating consumption of ultra-processed foods, since this was related with increasing oxidative stress markers in clinical follow-up of IBD patients., Competing Interests: Declaration of competing interest All authors declare no conflicts of interest, financial or otherwise., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2023
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27. Vedolizumab in Mild-to-Moderate Crohn's Disease Patients Naïve to Biological Therapy: A Multicentric Observational Study.
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Dotti AZ, Magro DO, Vilela EG, Chebli JMF, Chebli LA, Steinwurz F, Argollo M, Carvalho NS, Parente JML, Lima MM, Parra RS, Perin RL, Flores C, Morsoletto EM, da Costa Ferreira S, Ludvig JC, Kaiser Junior RL, Faria MAG, Nicollelli GM, Andrade AR, Queiroz NSF, and Kotze PG
- Abstract
Background: In real-world experience, the number of patients using vedolizumab as first-line biological therapy was low. We aimed to evaluate the effectiveness and safety of vedolizumab in mild-to-moderate Crohn's disease (CD) biologic-naïve patients., Methods: We performed a retrospective multicentric cohort study with patients who had clinical activity scores (Harvey-Bradshaw Index [HBI]) measured at baseline and weeks 12, 26, 52, as well as at the last follow-up. Clinical response was defined as a reduction ≥3 in HBI, whereas clinical remission as HBI ≤4. Mucosal healing was defined as the complete absence of ulcers in control colonoscopies. Kaplan-Meier survival analysis was used to assess the persistence with vedolizumab., Results: From a total of 66 patients, 53% (35/66) reached clinical remission at week 12. This percentage increased to 69.7% (46/66) at week 26, and 78.8% (52/66) at week 52. Mucosal healing was achieved in 62.3% (33/53) of patients. Vedolizumab was well tolerated, and most adverse events were minor. During vedolizumab treatment, 3/66 patients underwent surgery., Conclusions: This study demonstrates the effectiveness and safety of vedolizumab as a first-line biological agent in patients with mild-to-moderate CD., Competing Interests: P.G.K. and N.S.F.Q. hold the position of Associate Editor for Crohn’s & Colitis 360 and has been recused from reviewing or making decisions for the manuscript. A.Z.D. received a funding to a Master’s Degree scholarship for 2 years. D.O.M., E.G.V., M.M.L., R.L.P., R.L.K.J., and M.A.G.F. have nothing to declare. J.M.F.C. served as speaker for Abbott, Abbvie, Janssen and Takeda. LAC e ARA served as speaker for Janssen, Takeda, Abbvie. F.S. served as Advisory board member of Eurofarma, Janssen, Pfizer, Takeda, and speaker for AbbVie, Amgen, Ferring, Janssen, Pfizer, Sandoz, and Takeda. M.A. served as speaker from Janssen, Takeda, Abbvie, and Pfizer. NSC served as speaker from Janssen, Abbvie, and Sandoz. J.M.L.P. and J.C.L. served as speaker for Takeda. R.S.P. received lecture fee[s] from AbbVie, Janssen, Takeda, Pfizer; is an advisory committee member for Janssen and AbbVie; and he also does clinical research for Janssen and Abbvie. C.F. served as speaker from Takeda, Abbvie, Janssen, Sandoz, Pfizer. E.M.M. served as speaker from Takeda, Janssen, and Ferring. S.C.F. and G.M.N. served as speaker for Takeda and Janssen. N.S.F.Q. served a speaker and advisory board member for Janssen, Takeda, and Abbvie. P.G.K. served as speaker and consultancy for Janssen, Takeda, Abbvie, and Pfizer., (© The Author(s) 2023. Published by Oxford University Press on behalf of Crohn's & Colitis Foundation.)
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- 2023
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28. PAtCH: Proactive Approach to Circumvent Holes in Wireless Sensor Networks.
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Lima MM, Sardinha ED, Balico LN, and Oliveira HABF
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The occurrence of hole regions in Wireless Sensor Networks is a significant challenge when applying a greedy technique in a geographic routing approach. The local minimum phenomenon is commonly attributed to physical obstacles, energy depletion of the nodes, failures in communication between neighbors, or even the incorrect deployment of the nodes in the sensing field. To address the problem of hole regions, most approaches choose to abandon the traditional greedy forwarding mechanism to temporarily adopt the well-known perimeter routing scheme applied to nearby nodes or along the edge of a region of a hole. However, this mechanism does not satisfy the network load balance requirement, because it imposes too much traffic to the nodes in the hole's edge, making them overloaded when compared to other network nodes more distant from holes. In this work, we propose a novel location-free geographic routing technique called PAtCH (Proactive Approach to Circumvent Holes in Wireless Sensor Network) to avoid routing holes in WSNs. Our solution can circumvent hole regions and create routing paths toward the destination. We consider that our sink has a higher communication range, and the Received Signal Strength Indicator (RSSI) is used to assist the construction of the routing paths. Our results show the efficiency achieved by our proposed solution in scenarios with hole regions, also maintaining all the benefits of a classic greedy forwarding technique.
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- 2023
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29. Emergence of melioidosis in Brazil: a case series.
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Velôso DS, da Silva SP, de Coelho CMS, Parente JML, Veloso TAE, Lima MM, Sampaio CT, de Freitas MFAB, Rolim DB, de Lemos ERS, and Horta MAP
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- Adult, Humans, Abdominal Pain, Anti-Bacterial Agents therapeutic use, Brazil epidemiology, Burkholderia pseudomallei, Melioidosis diagnosis, Melioidosis drug therapy
- Abstract
Background: Melioidosis is a serious disease caused by the bacterium Burkholderia pseudomallei which affects humans and animals. It results in a wide spectrum of clinical manifestations, mainly in the respiratory tract, progressing to septic shock and death., Case Presentation: Herein, we present a series of seven patients (median age: 41 years) with confirmed melioidosis, treated at a public hospital in Piauí State, Brazil between 2019 and 2021. The most common clinical presentations were fever, cough, pneumonia, and abdominal pain. The mean duration of antibacterial therapy with 1 g of meropenem was 28.6 ± 1.1 days. Six patients recovered and one died. The mean hospitalization time was 51.0 ± 39.2 days., Conclusions: Melioidosis is an emerging infectious disease in Brazil. Health professionals in endemic areas need to be aware of the clinical presentation and appropriate clinical management of the disease because early diagnosis and early initiation of antibiotic therapy can be life-saving., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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30. SBC Guideline on the Diagnosis and Treatment of Patients with Cardiomyopathy of Chagas Disease - 2023.
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Marin-Neto JA, Rassi A Jr, Oliveira GMM, Correia LCL, Ramos Júnior AN, Luquetti AO, Hasslocher-Moreno AM, Sousa AS, Paola AAV, Sousa ACS, Ribeiro ALP, Correia Filho D, Souza DDSM, Cunha-Neto E, Ramires FJA, Bacal F, Nunes MDCP, Martinelli Filho M, Scanavacca MI, Saraiva RM, Oliveira Júnior WA, Lorga-Filho AM, Guimarães AJBA, Braga ALL, Oliveira AS, Sarabanda AVL, Pinto AYDN, Carmo AALD, Schmidt A, Costa ARD, Ianni BM, Markman Filho B, Rochitte CE, Macêdo CT, Mady C, Chevillard C, Virgens CMBD, Castro CN, Britto CFPC, Pisani C, Rassi DDC, Sobral Filho DC, Almeida DR, Bocchi EA, Mesquita ET, Mendes FSNS, Gondim FTP, Silva GMSD, Peixoto GL, Lima GG, Veloso HH, Moreira HT, Lopes HB, Pinto IMF, Ferreira JMBB, Nunes JPS, Barreto-Filho JAS, Saraiva JFK, Lannes-Vieira J, Oliveira JLM, Armaganijan LV, Martins LC, Sangenis LHC, Barbosa MPT, Almeida-Santos MA, Simões MV, Yasuda MAS, Moreira MDCV, Higuchi ML, Monteiro MRCC, Mediano MFF, Lima MM, Oliveira MT, Romano MMD, Araujo NNSL, Medeiros PTJ, Alves RV, Teixeira RA, Pedrosa RC, Aras Junior R, Torres RM, Povoa RMDS, Rassi SG, Alves SMM, Tavares SBDN, Palmeira SL, Silva Júnior TLD, Rodrigues TDR, Madrini Junior V, Brant VMDC, Dutra WO, and Dias JCP
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- Humans, Chagas Disease complications, Chagas Disease diagnosis, Chagas Disease therapy, Cardiomyopathies diagnosis, Cardiomyopathies therapy, Chagas Cardiomyopathy diagnosis, Chagas Cardiomyopathy therapy
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- 2023
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31. Phylogenetic Reconstructions Reveal the Circulation of a Novel Dengue Virus-1V Clade and the Persistence of a Dengue Virus-2 III Genotype in Northeast Brazil.
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Fritsch H, Moreno K, Lima IAB, Santos CS, Costa BGG, de Almeida BL, Dos Santos RA, Francisco MVLO, Sampaio MPS, de Lima MM, Pereira FM, Fonseca V, Tosta S, Xavier J, de Oliveira C, Adelino T, de Mello ALES, Gräf T, Alcantara LCJ, Giovanetti M, and de Siqueira IC
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- Humans, Phylogeny, Brazil epidemiology, Genetic Variation, RNA, Viral genetics, Genotype, Dengue Virus genetics, Dengue epidemiology
- Abstract
Dengue fever is among the most significant public health concerns in Brazil. To date, the highest number of Dengue notifications in the Americas has been reported in Brazil, with cases accounting for a total number of 3,418,796 reported cases as of mid-December 2022. Furthermore, the northeastern region of Brazil registered the second-highest incidence of Dengue fever in 2022. Due to the alarming epidemiological scenario, in this study, we used a combination of portable whole-genome sequencing, phylodynamic, and epidemiological analyses to reveal a novel DENV-1 genotype V clade and the persistence of DENV-2 genotype III in the region. We further report the presence of non-synonymous mutations associated with non-structural domains, especially the NS2A (non-structural protein 2A), as well as describe synonymous mutations in envelope and membrane proteins, distributed differently between clades. However, the absence of clinical data at the time of collection and notification, as well as the impossibility of monitoring patients in order to observe worsening or death, restricts our possibility of correlating mutational findings with possible clinical prognoses. Together, these results reinforce the crucial role of genomic surveillance to follow the evolution of circulating DENV strains and understand their spread across the region through inter-regional importation events, likely mediated by human mobility, and also the possible impacts on public health and outbreak management.
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- 2023
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32. Testicular alterations and semen quality in a selected group of breeding buffaloes.
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Albuquerque RDS, Monteiro FDO, Lima MM, Silva AOAD, Cunha MSE, Conceição M, Scheeren VFDC, Freitas CP, Papa FO, Monteiro BM, Viana RB, Coutinho LN, Miranda MDS, and Teixeira PPM
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- Animals, Cattle, Male, Breeding, Cryopreservation standards, Cryopreservation veterinary, Semen Preservation standards, Semen Preservation veterinary, Sperm Motility, Spermatozoa pathology, Ultrasonography veterinary, Buffaloes, Semen Analysis veterinary, Testis diagnostic imaging, Testis pathology
- Abstract
Testicular ultrasound enables the evaluation of changes in the testicular parenchyma. This study aimed to report the occurrence of hypoechogenic testicular alterations and their relationship with semen quality in five breeding buffaloes. Two buffaloes presented with hyperechoic points characteristic of fibrosis and anechoic density content between the parietal and visceral tunica. The two bulls without ultrasonographic changes showed higher average trajectory speed, linear velocity, curvilinear velocity, amplitude of lateral displacement of the spermatic head, total motility, progressive motility, fast speed, and acrosomal membrane values within the normal range. The number of spermatozoa with major and total defects was higher in the group of animals without alterations. The three buffaloes that presented with testicular alterations produced semen within established freezing standards.
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- 2023
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33. The compound event that triggered the destructive fires of October 2017 in Portugal.
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Ramos AM, Russo A, DaCamara CC, Nunes S, Sousa P, Soares PMM, Lima MM, Hurduc A, and Trigo RM
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Portugal is regularly affected by destructive wildfires that have severe social, economic, and ecological impacts. The total burnt area in 2017 (∼540,000 ha) marked the all-time record value since 1980 with a tragic toll of 114 fatalities that occurred in June and October events. The local insurance sector declared it was the costliest natural disaster in Portugal with payouts exceeding USD295 million. Here, the 2017 October event, responsible for more than 200,000 ha of burnt area and 50 fatalities is analyzed from a compound perspective. A prolonged drought led to preconditioned cumulative hydric stress of vegetation in October 2017. In addition, on 15 October 2017, two other major drivers played a critical role: 1) the passage of hurricane Ophelia off the Coast of Portugal, responsible for exceptional meteorological conditions and 2) the human agent, responsible for an extremely elevated number of negligent ignitions. This disastrous combination of natural and anthropogenic drivers led to the uncontrolled wildfires observed on 15 October., Competing Interests: The authors declare no competing interests., (© 2023 The Author(s).)
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- 2023
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34. Perinatal characteristics and longer-term outcomes in Brazilian children with confirmed or suspected congenital Zika infection: ZIKAction Paediatric Registry.
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de Siqueira IC, de Almeida BL, Lage MLC, Serra L, Carvalho A, de Lima MM, Góes MFN, Crispim MSIN, da Costa Pereira MM, Costa BGG, Bailey H, Byrne T, Giaquinto C, Fernandes G, Ruiz-Burga E, and Thorne C
- Abstract
Background: Despite growing scientific knowledge of Zika virus (ZIKV) infection, questions remain regarding ZIKV infection in pregnancy and congenital ZIKV syndrome (CZS)., Methods: The ZIKAction Paediatric Registry is an international registry of children with documented ZIKV exposure in utero and/or with confirmed or suspected CZS. Its aim is to characterize these children (i.e., clinical, radiological, neurodevelopmental features) and describe outcomes, longer-term sequelae and management through retrospective case note review. This analysis described the maternal and perinatal characteristics of children in the Registry's Bahia arm, assessed their neuroimaging, ophthalmic, hearing and electroencephalography abnormalities by microcephaly classification and reported on hospitalisations. Children born in 2015-2018 and enrolled 2020-2021 in three public health facilities in Salvador were included., Results: Of 129 (57% female) children, 15 (11·6%) had laboratory-confirmed congenital ZIKV infection and 114 (88·4%) suspected CZS. At delivery, 15 (11·6%) were normocephalic, 30 (23·3%) moderately microcephalic, and 84 (65·1%) severely microcephalic. Median birth head circumference z-score was -3·51 [IQR, -4·69,-2·73]. During follow-up, all children had abnormal neuroimaging, 80·3% (94/117) abnormal electroencephalogram, 62·2% (77/120) ophthalmic abnormalities, and 27·4% (34/124) hearing impairment. Microcephaly classification was significantly associated with gestational age, and ophthalmological and electroencephalography abnormalities. Of 125 children with hospitalisation data, 52 (41·6%) had been hospitalised by most recent follow-up, at median age of 15·8 [4·0, 34·4] months; infections were the leading cause., Conclusion: Congenital ZIKV infection is an emerging disease with a varied and incompletely understood spectrum. Continued long-term follow-up is essential to understand longer-term prognosis and to inform future health and educational needs., Competing Interests: Claire Thorne and all authors reports financial support was provided by European Commission Horizon 2020., (© 2023 The Authors.)
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- 2023
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35. Enhancing the expression of multi-antigen chimeric TGAGS/BST protein from Toxoplasma gondii in Escherichia coli BL 21 Star during batch cultivation.
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Bivar Matias SC, de Azevedo B, da Costa Filho JDB, Lima MM, Moura AD, Arantes Martins DR, de Sousa Júnior FC, and Santos ESD
- Subjects
- Animals, Antigens, Protozoan, Culture Media metabolism, Escherichia coli genetics, Escherichia coli metabolism, Glycerol metabolism, Humans, Isopropyl Thiogalactoside metabolism, Protozoan Proteins, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Recombinant Proteins metabolism, Escherichia coli Infections, Toxoplasma, Toxoplasmosis diagnosis
- Abstract
Toxoplasmosis, despite advances in science and technology, is a disease that requires attention since there is no vaccine capable of immunizing humans and animals against all isolated types of Toxoplasma gondii. Thus, the use of chimeric proteins, which can contain multiple antigens, is a very promising alternative for the process of obtaining a vaccine and diagnostic test for toxoplasmosis due to the great diversity of antigens presented by T. gondii. In this context, the present study evaluates batch culture strategies in the production of the multi-antigenic chimeric protein TgAGS/BsT from Toxoplasma gondii. Several exploratory cultures were initially carried out to observe the kinetic behavior of E. coli BL21 Star in five different medium compositions without the addition of IPTG (inducer). Cultures of E. coli B21 Star were carried out with 1.0 mM IPTG at different times of initiation of induction (0.5, 1, and 6 h) to evaluate the effects on cell growth, production of the protein of interest, culture pH, and acetic acid formation. The results showed that among the culture media evaluated, 2xTY and TB supplemented with glycerol had the best cell concentration values of 3.42 ± 0.05 g/L and 5.48 ± 0.05 g/L, respectively. In the assays induced by IPTG, a higher expression of TgAGS/BsT protein was observed, with induction beginning within 6 h of culture, with a maximum concentration of protein of interest of 1.82 ± 0.02 g/L for the 2xTY and 2.49 ± 0.03 g/L for the TB medium. In addition, later induction by IPTG provided greater stability of plasmid pET-TgAGS, remaining with values above 90% at the end of culture., Competing Interests: Declaration of competing interest Authors declare no conflict of interest., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2023
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36. Clinical significance of mitochondrial DNA content in acute promyelocytic leukaemia.
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Pereira-Martins DA, Coelho-Silva JL, Weinhäuser I, Franca-Neto PL, Silveira DR, Ortiz C, Moreira-Aguiar A, Lima MM, Koury LC, de Melo RA, Glória AB, Fagundes EM, Lino BK, Pagnano K, Bittencourt R, Nunes E, Traina F, Figueiredo-Pontes L, Keating A, Tallman MS, Ribeiro RC, Dilon R, Ganser A, Sanz MA, Berliner N, Valk P, Löwenberg B, Ottone T, Noguera NI, Voso MT, Paoloni F, Fazi P, Ammatuna E, Huls G, Schuringa JJ, Rego EM, and Lucena-Araujo AR
- Subjects
- Humans, Tretinoin therapeutic use, DNA, Mitochondrial genetics, Clinical Relevance, Neoplasm Recurrence, Local drug therapy, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Treatment Outcome, Leukemia, Promyelocytic, Acute drug therapy, Leukemia, Promyelocytic, Acute genetics
- Abstract
Although a growing body of evidence demonstrates that altered mtDNA content (mtDNAc) has clinical implications in several types of solid tumours, its prognostic relevance in acute promyelocytic leukaemia (APL) patients remains largely unknown. Here, we show that patients with higher-than-normal mtDNAc had better outcomes regardless of tumour burden. These results were more evident in patients with low-risk of relapse. The multivariate Cox proportional hazard model demonstrated that high mtDNAc was independently associated with a decreased cumulative incidence of relapse. Altogether, our data highlights the possible role of mitochondrial metabolism in APL patients treated with ATRA., (© 2022 British Society for Haematology and John Wiley & Sons Ltd.)
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- 2023
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37. Characteristics and temporal trends of mortality rates in children and adolescents in Mato Grosso and Brazil, 2009-2020.
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Lima MM, Favacho ARM, Souza-Santos R, and Gama SGND
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- Adolescent, Child, Female, Humans, Male, Brazil epidemiology, Mortality trends
- Abstract
Objective: to analyze the characteristics and temporal trend of mortality rates in the population aged 5 to 14 years in Mato Grosso state and in Brazil, from 2009 to 2020., Methods: this was an ecological time-series study, based on data taken from the Mortality Information System. Descriptive and trend analyses were performed, using the joinpoint regression model and calculating the average annual percentage change (AAPC)., Results: in Brazil and in Mato Grosso state, deaths were predominantly male, preventable and due to external causes. A falling trend was identified for Brazil (5-9 years AAPC: -2.9; 95%CI -4.3;-1.6 and 10-14 years AAPC: -2.5; 95%CI -3.3;-1.8), while a stationary trend was found in Mato Grosso (5-9 years AAPC: -2.0; 95%CI -5.6;1.7 and 10-14 years AAPC: -0.1; 95%CI -5.9;6.1)., Conclusion: the stable trend of mortality at high levels demands urgent interventions to reduce it.
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- 2022
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38. On-chip generation and dynamic piezo-optomechanical rotation of single photons.
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Bühler DD, Weiß M, Crespo-Poveda A, Nysten EDS, Finley JJ, Müller K, Santos PV, de Lima MM Jr, and Krenner HJ
- Abstract
Integrated photonic circuits are key components for photonic quantum technologies and for the implementation of chip-based quantum devices. Future applications demand flexible architectures to overcome common limitations of many current devices, for instance the lack of tuneabilty or built-in quantum light sources. Here, we report on a dynamically reconfigurable integrated photonic circuit comprising integrated quantum dots (QDs), a Mach-Zehnder interferometer (MZI) and surface acoustic wave (SAW) transducers directly fabricated on a monolithic semiconductor platform. We demonstrate on-chip single photon generation by the QD and its sub-nanosecond dynamic on-chip control. Two independently applied SAWs piezo-optomechanically rotate the single photon in the MZI or spectrally modulate the QD emission wavelength. In the MZI, SAWs imprint a time-dependent optical phase and modulate the qubit rotation to the output superposition state. This enables dynamic single photon routing with frequencies exceeding one gigahertz. Finally, the combination of the dynamic single photon control and spectral tuning of the QD realizes wavelength multiplexing of the input photon state and demultiplexing it at the output. Our approach is scalable to multi-component integrated quantum photonic circuits and is compatible with hybrid photonic architectures and other key components for instance photonic resonators or on-chip detectors., (© 2022. The Author(s).)
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- 2022
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39. Mechanical ventilation during cardiopulmonary bypass in neonates improves postoperative outcome.
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Rodríguez-Lima MM, González-Calle A, Adsuar-Gómez A, Sánchez-Martín MJ, Sepúlveda Iturzaeta Á, Sánchez-Valderrábanos E, García-Hernández JA, Murillo-Pozo MÁ, Ordóñez-Fernández A, and Hosseinpour AR
- Subjects
- Adult, Cardiopulmonary Bypass adverse effects, Cardiopulmonary Bypass methods, Child, Humans, Infant, Newborn, Prospective Studies, Respiration, Artificial, Treatment Outcome, Heart Septal Defects, Ventricular surgery, Transposition of Great Vessels surgery
- Abstract
Objectives: Cardiopulmonary bypass generates a systemic inflammatory response. This inflammatory response is reduced if patients are ventilated during bypass, as evidenced by lower levels of postoperative circulating inflammatory mediators. However, this does not appear to make much clinical difference in adults, at least not consistently, but, to our knowledge, has never been assessed in paediatric cardiac surgery, which is the objective of this study., Methods: This is a prospective clinical study of 12 consecutive neonates operated for the correction of either transposition of the great arteries ± ventricular septal defect or aortic arch hypoplasia ± ventricular septal defect, who were ventilated during cardiopulmonary bypass. These were compared to 11 neonates with the same malformations, who had undergone the same operations but without being ventilated during bypass (historical control group)., Results: One patient from the control group died on the 15th postoperative day due to sepsis and multi-organ failure. Bypass times and cross-clamp times were similar in the 2 groups. Ventilation on bypass was associated with significantly lower postoperative serum concentrations of C-reactive protein, shorter mechanical ventilation and lower vasoactive-inotropic score. Duration of stay on intensive care unit (ICU) showed a tendency to be shorter in patients who were ventilated on bypass, but this did not reach statistical significance. There were no differences between the 2 groups with respect to postoperative mixed venous oxygen saturations and serum concentrations of lactate and troponin I., Conclusions: Mechanical ventilation during cardiopulmonary bypass in neonates improves postoperative outcome., (© The Author(s) 2022. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.)
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- 2022
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40. Response to Chagas disease in Brazil: strategic milestones for achieving comprehensive health care.
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Ramos AN Jr, Souza EA, Guimarães MCS, Vermeij D, Cruz MM, Luquetti AO, Diotaiuti L, Palmeira SL, Lima MM, Costa VMD, Andrade LAB, Correia D, and Sousa AS
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- Brazil epidemiology, Comprehensive Health Care, Humans, Chagas Disease epidemiology, Chagas Disease prevention & control, Trypanosoma cruzi
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- 2022
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41. Correlation Between Functional Health Literacy and Self-efficacy in People with Type 2 Diabetes Mellitus: Cross-sectional Study.
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Medina LAC, Silva RA, de Sousa Lima MM, Barros LM, Lopes ROP, Melo GAA, Garcia Lira Neto JC, and Caetano JÁ
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- Cross-Sectional Studies, Humans, Self Care, Self Efficacy, Surveys and Questionnaires, Diabetes Mellitus, Type 2, Health Literacy
- Abstract
To analyze the correlation between functional health literacy (FHL) and self-efficacy (SE) in people with type 2 Diabetes Mellitus. Cross-sectional study was conducted among September and October 2019, with 196 people with type 2 diabetes. Data were collected using the Functional Literacy in Health instrument (B-TOFHLA) and the Diabetes Management Self-Efficacy Scale for Patients with Type 2 Diabetes Mellitus (DMSES). Bivariate analysis was used to verify the relationship among the constructs. Most diabetics showed an average B-TOFHLA score of 74.75, considered adequate, and self-efficacy of 4.07, high. The association between SE and FHL in the bivariate analysis found no statistical significance ( p > .05), in the same sense as the B-TOFHLA score and the DMSES domains ( p > .05). Constructs were not related to each other in terms of skills arising from judgments and decisions with motivational confidence by the investigated audience.
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- 2022
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42. Effect of dietary interventions on inflammatory biomarkers of inflammatory bowel diseases: A systematic review of clinical trials.
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Barros VJDS, Severo JS, Mendes PHM, da Silva ACA, de Oliveira KBV, Parente JML, Lima MM, Neto EMM, Aguiar Dos Santos A, and Tolentino Bento da Silva M
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- Biomarkers, Humans, Inflammation, Colitis, Ulcerative, Crohn Disease, Inflammatory Bowel Diseases
- Abstract
Objectives: In this review, we systematically assess whether dietary interventions are effective in attenuating inflammatory biomarkers in IBDs based on clinical trials available in the literature., Research Methods & Procedures: This review was conducted in accordance with the guidelines of the PRISMA. We used the PubMed and SciVerse Scopus databases and the Cochrane collaboration tool to assess the risk of bias in clinical trials. The PICO (patient, intervention, comparison, and outcomes) strategy was used, with the descriptors: "Inflammatory bowel disease", "Crohn's disease", "cd", "ibd", "ulcerative colitis", "uc", "Diet", "Diet Habits", "Feeding", "Nutrients", "Food Intake", "Dietary patterns", "Inflammations", "Inflammation", "acute-phase proteins", "C-reactive protein", "interleukins", "tumor necrosis factor-alpha" and "inflammatory response". There is no conflict of interest., Data Analysis: Fifteen studies were included, with a total of 627 participants. Of the total studies included, seven showed a reduction in some inflammatory markers in response to dietary interventions. This review was registered with the PROSPERO platform under number: CRD42021235150., Conclusions: The results presented in this review reveal that dietary intervention with specific characteristics may be important during the treatment of the inflammatory process in patients with IBDs., (Copyright © 2021 Elsevier Inc. All rights reserved.)
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- 2021
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43. Cultural adaptation and updating of the Risk assessment and management of exposure of health care workers in the context of covid-19 questionnaire.
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Marziale MHP, Rocha FLR, Cassenote AJF, Robazzi MLDCC, Palha PF, Ballestero JGA, Terra FS, Mininel VA, Santos HECD, Fracarolli IFL, Garcia GPA, Fortunato MAB, and Lima MM
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- Brazil, Cross-Cultural Comparison, Health Personnel, Humans, Risk Assessment, SARS-CoV-2, Surveys and Questionnaires, Translations, COVID-19
- Abstract
Objective: to translate and culturally adapt the Risk assessment and management of exposure of health care workers in the context of covid-19 questionnaire to the Brazilian context and to develop and evaluate a sociodemographic and occupational characterization questionnaire to compose the adapted questionnaire., Method: five stages were conducted to adapt the Risk assessment and management of exposure of health care workers in the context of covid-19 questionnaire, namely: translation, synthesis of the translations, evaluation by a committee of judges, back translation and pre-test. As for the complementary questionnaire, it was elaborated and evaluated by a committee of judges and a pre-test was carried out., Results: the questionnaires were validated and the pre-test stage was conducted with health workers and students., Conclusion: the final version adapted to the Brazilian context was called Questionário de avaliação de risco e gestão da exposição de trabalhadores e estudantes de saúde no contexto da covid-19 and is available for use, together with the final version of the Sociodemographic and occupational questionnaire: Risk assessment and management of exposure of health care workers and students in the context of covid-19. These questionnaires may assist in mitigating the risks of infection, illness and death of health workers and students due to covid-19.
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- 2021
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44. Genomic surveillance activities unveil the introduction of the SARS-CoV-2 B.1.525 variant of interest in Brazil: Case report.
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Pereira F, Tosta S, Lima MM, Reboredo de Oliveira da Silva L, Nardy VB, Gómez MKA, Lima JG, Fonseca V, de Oliveira T, Lourenço J, Alcantara LC Jr, Giovanetti M, and Leal A
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- Aged, Brazil epidemiology, COVID-19 diagnosis, COVID-19 epidemiology, Communicable Diseases, Imported diagnosis, Communicable Diseases, Imported epidemiology, Female, Genome, Viral genetics, Humans, Mutation, Nigeria epidemiology, Travel-Related Illness, COVID-19 virology, Communicable Diseases, Imported virology, SARS-CoV-2 genetics, SARS-CoV-2 isolation & purification
- Abstract
The appearance of new variants of SARS-CoV-2 has recently challenged public health authorities with respect to tracking transmission and mitigating the impact in the evolving pandemic across countries. B.1.525 is considered a variant under investigation since it carries specific genetic signatures present in P.1, B.1.1.7, and B.1.351. Here we report genomic evidence of the first likely imported case of the SARS-CoV-2 B.1.525 variant, isolated in a traveler returning from Nigeria., (© 2021 Wiley Periodicals LLC.)
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- 2021
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45. [Stratification of priority territories for surveillance of chronic Chagas disease: multicriteria analysis for decision-making in health].
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Lima MM, Costa VMD, Palmeira SL, and Castro APB
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- Brazil epidemiology, Humans, Quality of Life, Reproducibility of Results, Chagas Disease epidemiology, Trypanosoma cruzi
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The identification of the magnitude of chronic Chagas disease in Brazil requires linking activities in health surveillance, seeking to develop a wide, hierarchically organized and geographically distributed network of services to provide care to thousands of individuals with Trypanosoma cruzi infection. The study aimed to elaborate a model for prioritization of municipalities for chronic Chagas disease, to offer comprehensive care for persons with the disease. A multicriteria analysis was thus performed using the PROMETHEÉ II algorithm, implemented in the Pradin software. The criteria for assessing the model consisted of three indices built from the following indicators: (a) epidemiological, directly related to chronic Chagas disease, (b) related to the evolution in chronic Chagas disease, and (c) related to access to health services. Saaty's Fundamental Scale was used to define the indicators' weights, with greater importance assigned to those directly related to chronic Chagas disease and to those with greater reliability and respective quality of information. Assessment of the models' consistency was based on comparison of the available data in historically endemic areas with the distribution of acute cases, besides other sensitivity analyses. The best model was defined by 1,345 municipalities with medium priority, 1,003 high priority, and 601 with very high priority for chronic Chagas disease, with the highest proportions in the Southeast and Northeast regions. Prioritization allows the administration to rationalize and channel resources, and it is essential to identify the territories where persons with chronic Chagas disease are living, to promote comprehensive care and improve quality of life.
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- 2021
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46. Real-world treatment patterns and disease control over one year in patients with inflammatory bowel disease in Brazil.
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Sassaki LY, Miszputen SJ, Kaiser Junior RL, Catapani WR, Bafutto M, Scotton AS, Zaltman C, Baima JP, Ramos HS, Faria MAG, Gonçalves CD, Guimaraes IM, Flores C, Amarante HMBS, Nones RB, Parente JML, Lima MM, Chebli JM, Ferrari MLA, Campos JF, Sanna MGP, Ramos O, Parra RS, da Rocha JJR, Feres O, Feitosa MR, Caratin RF, Senra JT, and Santana GO
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- Adult, Brazil epidemiology, Humans, Middle Aged, Prospective Studies, Quality of Life, Colitis, Ulcerative diagnosis, Colitis, Ulcerative drug therapy, Colitis, Ulcerative epidemiology, Inflammatory Bowel Diseases
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Background: Crohn's disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases (IBDs) with a remission-relapsing presentation and symptomatic exacerbations that have detrimental impacts on patient quality of life and are associated with a high cost burden, especially in patients with moderate-to-severe disease. The Real-world Data of Moderate-to-Severe Inflammatory Bowel Disease in Brazil (RISE BR) study was a noninterventional study designed to evaluate disease control, treatment patterns, disease burden and health-related quality of life in patients with moderate-to-severe active IBD. We report findings from the prospective follow-up phase of the RISE BR study in patients with active UC or CD., Aim: To describe the 12-mo disease evolution and treatment patterns among patients with active moderate-to-severe IBD in Brazil., Methods: This was a prospective, noninterventional study of adult patients with active Crohn's disease (CD: Harvey-Bradshaw Index ≥ 8, CD Activity Index ≥ 220), inadequate CD control ( i.e., calprotectin > 200 µg/g or colonoscopy previous results), or active ulcerative colitis (UC: Partial Mayo score ≥ 5). Enrollment occurred in 14 centers from October 2016 to February 2017. The proportion of active IBD patients after 9-12 mo of follow-up, Kaplan-Meier estimates of the time to mild or no activity and a summary of treatment initiation, discontinuation and dose changes were examined., Results: The study included 118 CD and 36 UC patients, with mean ± SD ages of 43.3 ± 12.6 and 44.9 ± 16.5 years, respectively. The most frequent drug classes at index were biologics for CD (62.7%) and 5-aminosalicylate derivates for UC patients (91.7%). During follow-up, 65.3% of CD and 86.1% of UC patients initiated a new treatment at least once. Discontinuations/dose changes occurred in 68.1% of CD patients [median 2.0 (IQR: 2-5)] and 94.3% of UC patients [median 4.0 (IQR: 3-7)]. On average, CD and UC patients had 4.4 ± 2.6 and 5.0 ± 3.3 outpatient visits, respectively. The median time to first mild or no activity was 319 (IQR: 239-358) d for CD and 320 (IQR: 288-358) d for UC patients. At 9-12 mo, 22.0% of CD and 20.0% of UC patients had active disease., Conclusion: Although a marked proportion of active IBD patients achieved disease control within one year, the considerable time to achieve this outcome represents an unmet medical need of the current standard of care in a Brazilian real-world setting., Competing Interests: Conflict-of-interest statement: Ligia Yukie Sassaki has received fees for serving as a speaker for AbbVie and Takeda. Sender Jankiel Miszputen has received fees for serving as a speaker and/or a consultant for Farmoquimica, Janssen and Marjan. He has received research funding from Ache, Roche and Takeda. Wilson Roberto Catapani has received fees for serving as a speaker and/or an advisory board member for Janssen and Takeda. Mauro Bafutto has received fees for serving as a speaker for Takeda, AbbVie, Janssen, UCB and Farmoquimica. He has received fees for serving as an advisory board member for AbbVie and Janssen. Antonio Scafuto Scotton has received fees for serving as a speaker for Janssen, Novartis, AbbVie, MSD, EMS. He has received research funding from Janssen, Novartis, AbbVie, Roche, Pfizer, Bristol, Lilly, Novo Nordisk, Anthera, AstraZeneca, GSK, UCB, Sanofi, Takeda, Parexel, IQVIA, PPD, PRA, ICON, INP Research, Covance, In Trials. Cyrla Zaltman has received fees for serving as a speaker for UCB, Janssen, Takeda, AbbVie. She has received research funding from AbbVie, Takeda, Janssen. Julio Pinheiro Baima has received fees for serving as a speaker for Janssen and Takeda. Cristina Flores has received fees for serving as a speaker for Janssen, Takeda, AbbVie. She has received fees for serving as an advisory board member for Janssen. Rodrigo Bremer Nones has received fees for serving as a speaker for AbbVie, Ferring Pharmaceuticals, Janssen, Nestle, Novartis, Pfizer, UCB Pharma and Takeda. Jose Miguel Luz Parente has received fees for serving as a speaker for Takeda. Julio Maria Fonseca Chebli has received fees for serving as a speaker for AbbVie, Janssen, UCB Pharma and Takeda. Maria de Lourdes de Abreu Ferrari has received fees for serving as a speaker and/or advisory board member for AbbVie, Ferring Pharmaceuticals, Janssen, UCB Pharma, and Takeda. Rogerio Serafim Parra has received fees for serving as a speaker and/or an advisory board member for AbbVie, Ferring Pharmaceuticals, Janssen, UCB Pharma and Takeda. Jose Joaquim Ribeiro da Rocha has received fees for serving as a speaker for Nestle. Marley Ribeiro Feitosa has received fees for serving as a speaker for AbbVie and Janssen. Rosana Fusaro Caratin was an employee of Takeda Pharmaceuticals Brazil at the time the manuscript was developed. Juliana Tosta Senra is an employee of Takeda Pharmaceuticals Brazil. Genoile Oliveira Santana has received fees for serving as a speaker for Takeda, AbbVie, Janssen, UCB Pharma. She has received research funding from Celgene and Roche. She has received fees for serving as an advisory board member for Janssen. No conflict of interest: Roberto Luiz Kaiser Junior, Mikaell Alexandre Gouvea Faria, Carolina Dias Goncalves, Isabella de Miranda Guimaraes, Heda Maria Barska dos Santos Amarante, Murilo Moura Lima, Odery Ramos, Omar Feres, Hagata Souza Ramos, Julia Faria Campos, and Maria das Gracas Pimenta Sanna., (©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.)
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- 2021
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47. Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil.
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Adelino TÉR, Giovanetti M, Fonseca V, Xavier J, de Abreu ÁS, do Nascimento VA, Demarchi LHF, Oliveira MAA, da Silva VL, de Mello ALES, Cunha GM, Santos RH, de Oliveira EC, Júnior JAC, de Melo Iani FC, de Filippis AMB, de Abreu AL, de Jesus R, de Albuquerque CFC, Rico JM, do Carmo Said RF, Silva JA, de Moura NFO, Leite P, Frutuoso LCV, Haddad SK, Martínez A, Barreto FK, Vazquez CC, da Cunha RV, Araújo ELL, de Oliveira Tosta SF, de Araújo Fabri A, Chalhoub FLL, da Silva Lemos P, de Bruycker-Nogueira F, de Castro Lichs GG, Zardin MCSU, Segovia FMC, Gonçalves CCM, Grillo ZDCF, Slavov SN, Pereira LA, Mendonça AF, Pereira FM, de Magalhães JJF, Dos Santos Júnior ACM, de Lima MM, Nogueira RMR, Góes-Neto A, de Carvalho Azevedo VA, Ramalho DB, Oliveira WK, Macario EM, de Medeiros AC, Pimentel V, Holmes EC, de Oliveira T, Lourenço J, and Alcantara LCJ
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- Brazil epidemiology, Dengue prevention & control, Dengue transmission, Dengue virology, Dengue Virus isolation & purification, Feasibility Studies, Genetic Variation, Genome, Viral genetics, Humans, Mobile Health Units, Molecular Epidemiology, Molecular Typing, Phylogeny, Proof of Concept Study, RNA, Viral genetics, RNA, Viral isolation & purification, Real-Time Polymerase Chain Reaction, Retrospective Studies, Whole Genome Sequencing, Dengue epidemiology, Dengue Virus genetics, Epidemics prevention & control, Epidemiological Monitoring
- Abstract
Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015-2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.
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- 2021
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48. Effects of glutamine supplementation on inflammatory bowel disease: A systematic review of clinical trials.
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Severo JS, da Silva Barros VJ, Alves da Silva AC, Luz Parente JM, Lima MM, Moreira Lima AÂ, Dos Santos AA, Matos Neto EM, and Tolentino Bento da Silva M
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- Clinical Trials as Topic, Dietary Supplements, Glutamine, Humans, Colitis, Ulcerative, Crohn Disease, Inflammatory Bowel Diseases drug therapy
- Abstract
Context: Glutamine supplementation has been applied clinical practice to treat inflammatory bowel disease (IBD). However, scientific evidence about this is still controversial., Objective: In this review, we systematically evaluated the effects of glutamine supplementation on IBD, based on evidence from randomized clinical trials., Data Source: This review was conducted in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We used the PubMed and SciVerse Scopus databases. The Cochrane collaboration tool was used to assess the risk of bias in clinical trials., Data Extraction: The review was carried out by two independent researchers according to the established inclusion criteria. The PICO (patient, intervention, comparison, and outcomes) strategy was used, with the descriptors: "glutamine," "supplementation," "inflammatory bowel diseases," "Crohn's disease," and "ulcerative colitis"., Data Synthesis: Seven research articles were selected for this systematic review. In these studies, glutamine was administered to the participants through oral (21-30g or 0.5g per kg of participant's body weight), enteral (7.87g-8.3 g/100g of the enteral formula), and/or parenteral (0.3 g/kg of the participant's body weight) routes. No changes in anthropometry or biochemical parameters were observed. However, in one study reduced intestinal permeability and morphometry were reported. In two other studies, a slight effect of glutamine on inflammation and oxidative stress was observed. Additionally, two other studies reported an effect of glutamine supplementation on disease activity., Conclusions: The findings obtained through this systematic review indicate that glutamine supplementation has no effect on disease course, anthropometric measurements, intestinal permeability and morphology, disease activity, intestinal symptoms, biochemical parameters, oxidative stress and inflammation markers in patients with IBD, regardless of the route of administration, either treated at a hospital or as outpatients., Competing Interests: Declaration of competing interest There is no conflict of interest., (Copyright © 2021 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved.)
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- 2021
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49. Insulin Resistance in Congenital Adrenal Hyperplasia is Compensated for by Reduced Insulin Clearance.
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de Oliveira DM, Tura A, Vasques ACJ, Camilo DF, Lima MM, de Lemos-Marini SHV, Goncalves EM, Guerra-Junior G, and Geloneze B
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- Adrenal Hyperplasia, Congenital complications, Adult, Cross-Sectional Studies, Female, Humans, Hyperinsulinism complications, Insulin-Secreting Cells metabolism, Male, Young Adult, Adrenal Hyperplasia, Congenital metabolism, Hyperinsulinism metabolism, Insulin metabolism, Insulin Resistance
- Abstract
Context: Congenital adrenal hyperplasia (CAH) patients have potential normal longevity. However, a greater risk for cardiovascular disease has been reported. Insulin resistance and hyperinsulinemia have been described in CAH patients, whereas the prevalence of overt type 2 diabetes is not higher in CAH than in normal population., Objective: To examine the contributions of insulin secretion and of hepatic insulin clearance to compensatory hyperinsulinemia in young insulin-resistant adults with classic CAH due to 21-hydroxylase deficiency (21-OHD)., Design: Cross-sectional., Setting: University outpatient clinics., Methods: Fifty-one participants: 21 controls, and 30 CAH (15 virilizing and 15 salt-wasting phenotypes), female/male (33/18), age (mean [SD]): 24.0 (3.6) years, body mass index: 24.6 (4.9)kg/m2 with normal glucose tolerance, were submitted to a hyperglycemic clamp study., Main Outcome Measures: Insulin sensitivity, beta cell function, and hepatic insulin clearance using appropriate modeling., Results: We found an increased insulin resistance in 21-OHD. The systemic hyperinsulinemia (posthepatic insulin delivery) was elevated in CAH patients. No increases were observed in insulin secretory rate (beta cell function) in the first phase or during the hyperglycemic clamp. The increase in insulin concentrations was totally due to a ~33% reduction in insulin clearance., Conclusion: 21-OHD nonobese subjects have reduced insulin sensitivity and beta cell response unable to compensate for the insulin resistance, probably due to overexposure to glucocorticoids. Compensatory hyperinsulinemia is most related with reduced hepatic insulin clearance. The exclusive adaptation of the liver acts as a gating mechanism to regulate the access of insulin to insulin-sensitive tissues to maintain glucose homeostasis., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2021
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50. The risk of oral transmission in an area of a Chagas disease outbreak in the Brazilian northeast evaluated through entomological, socioeconomic and schooling indicators.
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Monsalve-Lara J, Lilioso M, Valença-Barbosa C, Thyssen PJ, Miguel DC, Limeira C, Gadelha FR, Fontes FVHM, Pires-Silva D, Dornak LL, Lima MM, Donalisio MR, and Almeida CE
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- Animals, Brazil epidemiology, Chagas Disease epidemiology, Entomology, Humans, Insect Vectors, Prevalence, Risk Factors, Socioeconomic Factors, Chagas Disease transmission, Disease Outbreaks
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Chagas disease is a neglected tropical disease strongly associated with low socioeconomic status, affecting nearly 8 million people - mainly Latin Americans. The current infection risk is based on acute case reports, most of which are typically associated with oral transmissions. In the semi-arid region of Northeastern Brazil, serious outbreaks of this transmission type have surged in the last years. One of those occurred in 2016 in the state of Rio Grande do Norte. Rural residents of four municipalities surrounding Marcelino Vieira ingested sugar cane juice - which was probably ground with Trypanosoma cruzi-infected insects. Eighteen cases of Chagas disease were confirmed serologically, with two deaths reported. Socioeconomic information, schooling of residents and the structure of peridomestic and domestic environments in the rural area of Marcelino Vieira, along with entomological indicators, were investigated to understand better the factors related to the outbreaks in this region. We found triatomines (mainly Triatoma brasiliensis) in 35% (24/67) of domiciliary units and all rocky outcrops inspected (n = 7). Overall, 25% (91/357) of examined T. brasiliensis were infected by T. cruzi in artificial ecotopes, with almost the same prevalence in the sylvatic environment (22%; 35/154). Among all ecotopes investigated, wood/tile/brick piles were the ones linked to high insect infestations and triatomine T. cruzi infection prevalence. Ninety-five percent of people interviewed recognized the triatomines and knew the classic route of transmission of disease - triatomine bite-dependent. However, only 7.5% admitted knowledge that Chagas disease can also be acquired orally - which poses a risk this transmission route currently recognized. Here, we highlight the physical proximity between humans and triatomine populations with high T. cruzi infection prevalence as an additional risk factor to oral/vector contaminations. In sum, residents have low income, low level of education, and/or a willful disregard for the routes of Chagas disease transmission (specifically oral transmission), a combination of factors that may have favored the Chagas disease outbreak. We here provide recommendations to avoid further outbreaks., (Copyright © 2020. Published by Elsevier B.V.)
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- 2021
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