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159 results on '"Lim, Yun Kyong"'

24. The antibacterial effect of Endoseal TCS mixed with water-soluble mangostin derivatives of Garcinia mangostana L. ethanol extract against Enterococcus faecalis and Staphylococcus aureus

Author

Kim Jin-Hee, Kook Joong-Ki, Lim Yun-Kyong, Lee Dae-Sung, and Park Tae-Young

Subjects
Mineral trioxide aggregate, food.ingredient, biology, Chemistry, fungi, medicine.disease_cause, biology.organism_classification, Antimicrobial, Enterococcus faecalis, chemistry.chemical_compound, food, Staphylococcus aureus, medicine, Garcinia mangostana, Agar diffusion test, Food science, Antibacterial activity, Mangostin
Abstract

This study evaluated the antimicrobial activity of Endoseal TCS, an mineral trioxide aggregate-based root canal sealer, mixed with water-soluble mangostin derivatives (WsMD) of Garcinia mangostana L. (mangosteen) ethanol extract against Enterococcus faecalis and Staphylococcus aureus. The antibacterial activity of Endoseal TCS mixed with WsMD against three strains of E. faecalis and three strains of S. aureus was performed using agar diffusion test. The data showed that Endoseal TCS mixed with 0.115% WsMD had a zone of inhibition of 0.7 ± 0.2–2.4 ± 0.1 mm. The results suggest that Endoseal TCS mixed with WsMD of Garcinia mangostana L. ethanol extract is useful as a root canal sealer with antibacterial activity against E. faecalis and S. aureus .

Published
2021
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32. Antimicrobial effect of Australia propolis on cariogenic and periodontopathic bacteria

Author

Lim Yun-Kyong, Kook Joong-Ki, Lee Dae-Sung, and Yoo So-Young

Subjects
Periodontitis, Minimum bactericidal concentration, biology, business.industry, Aggregatibacter actinomycetemcomitans, Propolis, medicine.disease, Antimicrobial, biology.organism_classification, Microbiology, Antimicrobial effect, medicine, Infectious disease (athletes), business, Periodontopathic bacteria
Abstract

The purpose of this study was to investigate the antimicrobial effects of Australia propolis against cariogenic and periodontopathic bacteria. Antimicrobial activity was determined by evaluating the minimal bactericidal concentration (MBC). Cell cytotoxicity of propolis extract on normal human gingival fibroblast (HGF-1) cells was observed using the methylthiazolyldiphenyl-tetrazolium bromide assay. The data indicated that, with the exception of Aggregatibacter actinomycetemcomitans (KCOM 1306), the MBC values of the propolis strains were 0.25–1% without HGF-1 cell cytotoxicity. These results suggest that propolis can be used to develop oral hygiene products for the prevention of oral infectious disease.

Published
2019
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35. Performance assessment of ASTA MicroIDSys, a new matrix assisted laser desorption ionization‐time of flight mass spectrometry system, for identification of viridans group streptococci

Author

Lee, O‐Jin, primary, Ko, Young Jin, additional, Lee, Seul‐Bi, additional, Kim, Choon Mee, additional, Jang, Sook‐Jin, additional, Kook, Joong‐Ki, additional, Lim, Yun Kyong, additional, Shin, Jong Hee, additional, Shin, Myung Geun, additional, Kee, Seung Jung, additional, Jeong, Seok Hoon, additional, Kang, Seong‐Ho, additional, and Park, Geon, additional

Published
2021
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46. <italic>Capnocytophaga endodontalis</italic> sp. nov., Isolated from a Human Refractory Periapical Abscess.

Author

Jo, Eojin, Park, Soon-Nang, Lim, Yun Kyong, Paek, Jayoung, Shin, Yeseul, Kim, Hongik, Kim, Si Hyun, Shin, Jeong Hwan, Chang, Young-Hyo, and Kook, Joong-Ki

Subjects
CAPNOCYTOPHAGA, ABSCESSES, MANDIBLE abnormalities, NUCLEOTIDE sequence, BONE diseases
Abstract

A novel Gram-negative, capnophilic, fusiform bacterium, designated strain ChDC OS43T, was isolated from a human refractory periapical abscess in the left mandibular second molar and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene sequence revealed that the strain belongs to the genus Capnocytophaga, as it showed sequence similarities to Capnocytophaga ochracea ATCC 27872T (96.30%) and C. sputigena ATCC 33612T (96.16%). The prevalent fatty acids of strain ChDC OS43T were isoC15:0 (57.54%), C16:0 (5.93%), C16:0 3OH (5.72%), and C18:1cis 9 (4.41%). The complete genome of strain ChDC OS43T was 3,412,686 bp, and the G+C content was 38.2 mol%. The average nucleotide identity (ANI) value between strain ChDC OS43T and C. ochracea ATCC 27872T or C. sputigena ATCC 33612T was >92.01%. The genome-to-genome distance (GGD) value between strain ChDC OS43T and C. ochracea ATCC 27872T or C. sputigena ATCC 33612T was 32.0 and 45.7%, respectively. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC OS43T (= KCOM 1579T = KCTC 5562T = KCCM 42841T = JCM 32133T) should be classified as the type strain of a novel species of genus Capnocytophaga, for which the name Capnocytophaga endodontalis sp. nov. is proposed. [ABSTRACT FROM AUTHOR]

Published
2018
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48. Antimicrobial Mechanism of Oleanolic and Ursolic Acids on Streptococcus mutans UA159.

Author

Park, Soon-Nang, Lim, Yun Kyong, Choi, Mi-Hwa, Cho, Eugene, Bang, Iel Soo, Kim, Jung Min, Ahn, Sug-Joon, and Kook, Joong-Ki

Subjects
TRITERPENOID saponins, STREPTOCOCCUS mutans, ANTI-infective agents, URSOLIC acid, POLYMERASE chain reaction, PHYSIOLOGY
Abstract

Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. However, it is not clear whether this is the only pathway involved in the antimicrobial activity of these compounds against S. mutans. Therefore, we used quantitative real-time PCR (qPCR) and microarray analyses to examine the expression of genes related to essential metabolic pathways in S. mutans UA159 following incubation with OA, UA, or BA. An oligonucleotide array consisting of 5363 probes was designed to survey 1928 of the 1963 genes in the genome of S. mutans UA159. Genes that showed >2-fold changes in expression in response to the treatment conditions were annotated, and selected target genes involved in central metabolism were analyzed by qPCR. Microarray analysis confirmed that the gene expression patterns of the OA- and UA-treated cells differed from that of the BA-treated culture, indicating differences in the antimicrobial mechanism. In particular, the expression of pfk and pykF, coding for glycolysis regulatory proteins phosphofructokinase and pyruvate kinase, respectively, were significantly decreased in the OA and UA groups ( P < 0.05), as were genes involved in fatty acid and amino acid synthesis. In addition, the microarray analysis confirmed previous qPCR results showing that peptidoglycan synthesis is down-regulated in the OA- and UA-treated groups. OA and UA also appear to decrease the generation of organic acids by S. mutans UA159, which would have an anticaries effect. Overall, these findings suggest that OA and UA affect multiple genes involved in the central metabolism of S. mutans, with inhibition of glycolysis, fatty acid synthesis, amino acid synthesis, and peptidoglycan synthesis, all contributing to their antimicrobial activity. [ABSTRACT FROM AUTHOR]

Published
2018
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