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4. Vat Polymerization

Author

Murphy, Caroline A., primary, Alcala-Orozco, Cesar R., additional, Longoni, Alessia, additional, Woodfield, Tim B. F., additional, and Lim, Khoon S., additional

Published
2022
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8. Single-Atom-Based Nanoenzyme in Tissue Repair

Author

Fu, Ziliang, primary, Fan, Kexin, additional, He, Xingjian, additional, Wang, Qiguang, additional, Yuan, Jie, additional, Lim, Khoon S., additional, Tang, Jun-Nan, additional, Xie, Fangxi, additional, and Cui, Xiaolin, additional

Published
2024
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12. Harnessing Macromolecular Chemistry to Design Hydrogel Micro- and Macro-Environments

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Soliman, Bram G., Longoni, Alessia, Major, Gretel S., Lindberg, Gabriella C.J., Choi, Yu Suk, Zhang, Yu Shrike, Woodfield, Tim B.F., Lim, Khoon S., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Soliman, Bram G., Longoni, Alessia, Major, Gretel S., Lindberg, Gabriella C.J., Choi, Yu Suk, Zhang, Yu Shrike, Woodfield, Tim B.F., and Lim, Khoon S.

Published
2024

13. Mapping the microcarrier design pathway to modernise clinical mesenchymal stromal cell expansion

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Major, Gretel S., Doan, Vinh K., Longoni, Alessia, Bilek, Marcela M.M., Wise, Steven G., Rnjak-Kovacina, Jelena, Yeo, Giselle C., Lim, Khoon S., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Major, Gretel S., Doan, Vinh K., Longoni, Alessia, Bilek, Marcela M.M., Wise, Steven G., Rnjak-Kovacina, Jelena, Yeo, Giselle C., and Lim, Khoon S.

Published
2024

15. Design considerations for digital light processing bioprinters.

Author

Garciamendez-Mijares, Carlos Ezio, Aguilar, Francisco Javier, Hernandez, Pavel, Kuang, Xiao, Gonzalez, Mauricio, Ortiz, Vanessa, Riesgo, Ricardo A., Ruiz, David S. Rendon, Rivera, Victoria Abril Manjarrez, Rodriguez, Juan Carlos, Mestre, Francisco Lugo, Castillo, Penelope Ceron, Perez, Abraham, Cruz, Lourdes Monserrat, Lim, Khoon S., and Zhang, Yu Shrike

Subjects
BIOPRINTING, BIOMATERIALS, BEST practices, WORKFLOW, OPTICS
Abstract

With the rapid development and popularization of additive manufacturing, different technologies, including, but not limited to, extrusion-, droplet-, and vat-photopolymerization-based fabrication techniques, have emerged that have allowed tremendous progress in three-dimensional (3D) printing in the past decades. Bioprinting, typically using living cells and/or biomaterials conformed by different printing modalities, has produced functional tissues. As a subclass of vat-photopolymerization bioprinting, digital light processing (DLP) uses digitally controlled photomasks to selectively solidify liquid photocurable bioinks to construct complex physical objects in a layer-by-layer manner. DLP bioprinting presents unique advantages, including short printing times, relatively low manufacturing costs, and decently high resolutions, allowing users to achieve significant progress in the bioprinting of tissue-like complex structures. Nevertheless, the need to accommodate different materials while bioprinting and improve the printing performance has driven the rapid progress in DLP bioprinters, which requires multiple pieces of knowledge ranging from optics, electronics, software, and materials beyond the biological aspects. This raises the need for a comprehensive review to recapitulate the most important considerations in the design and assembly of DLP bioprinters. This review begins with analyzing unique considerations and specific examples in the hardware, including the resin vat, optical system, and electronics. In the software, the workflow is analyzed, including the parameters to be considered for the control of the bioprinter and the voxelizing/slicing algorithm. In addition, we briefly discuss the material requirements for DLP bioprinting. Then, we provide a section with best practices and maintenance of a do-it-yourself DLP bioprinter. Finally, we highlight the future outlooks of the DLP technology and their critical role in directing the future of bioprinting. The state-of-the-art progress in DLP bioprinter in this review will provide a set of knowledge for innovative DLP bioprinter designs. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Photocrosslinked Silk Fibroin Microgel Scaffolds for Biomedical Applications.

Author

Karimi, Fatemeh, Farbehi, Nona, Ziaee, Farzaneh, Lau, Kieran, Monfared, Marzieh, Kordanovski, Marija, Joukhdar, Habib, Molly, Thomas G., Nordon, Robert, Kilian, Kristopher A., Stenzel, Martina H., Lim, Khoon S., and Rnjak‐Kovacina, Jelena

Subjects
TISSUE scaffolds, SILK fibroin, MICROGELS, PHOTOCROSSLINKING, TISSUE engineering, MICROFLUIDIC devices, POLYCAPROLACTONE, SPIDER silk
Abstract

Silk fibroin hydrogels are extensively explored for tissue engineering and regenerative medicine as an artificial extracellular matrix (ECM) that can support tissue growth. However, the nanometer pore size of hydrogels limits adequate cell, tissue, and vascular infiltration. Microgel scaffolds are an emerging class of microporous biomaterials formed by annealing small microscale hydrogels (microgels) into a 3D construct. In this work, silk microgels are generated using a microfluidic device that allows tuning of the microgel diameter (100–400 µm) and are stabilized via visible light‐initiated photo‐crosslinking of native tyrosine residues in silk. Microgels are then covalently annealed using silk solution as glue and the same cytocompatible visible light‐initiated crosslinking to form microgel scaffolds. Unlike the nano‐porosity of bulk photo‐crosslinked silk hydrogels, the microgel scaffolds have an average pore diameter of 29 ± 17 or 192 ± 81 µm depending on the microgel size, with enhanced mechanical properties compared to bulk hydrogels. This microporosity supports enhanced cell spreading and proliferation in vitro and increases scaffold remodeling in vivo, encouraging improved tissue infiltration and matrix deposition. The microgel size and material format also affect inflammatory responses in vivo. This work demonstrates that silk microgels and microgel scaffolds are promising candidates for tissue engineering and regenerative medicine applications. [ABSTRACT FROM AUTHOR]

Published
2024
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33. 3D Volumetric Mechanosensation of MCF7 Breast Cancer Spheroids in a Linear Stiffness Gradient GelAGE

Author

Vahala, Danielle, primary, Amos, Sebastian E., additional, Sacchi, Marta, additional, Soliman, Bram G., additional, Hepburn, Matt S., additional, Mowla, Alireza, additional, Li, Jiayue, additional, Jeong, Ji Hoon, additional, Astell, Chrissie, additional, Hwang, Yongsung, additional, Kennedy, Brendan F., additional, Lim, Khoon S., additional, and Choi, Yu Suk, additional

Published
2023
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36. Harnessing light in biofabrication

Author

Levato, Riccardo, Lim, Khoon S., Levato, Riccardo, and Lim, Khoon S.

Abstract

The integration of light-driven technologies into biofabrication has revolutionized the field of tissue engineering and regenerative medicine, with numerous breakthroughs in the last few years. Light-based bioprinting approaches (lithography, multiphoton and volumetric bioprinting) have shown the potential to fabricate large scale tissue engineering constructs of high resolution, with great flexibility and control over the cellular organization. Given the unprecedented degree of freedom in fabricating convoluted structures, key challenges in regenerative medicine, such as introducing complex channels and pre-vascular networks in 3D constructs have also been addressed. Light has also been proven as a powerful tool, leading to novel photo-chemistry in designing bioinks, but also able to impart spatial-temporal control over cellular functions through photo-responsive chemistry. For instance, smart constructs able to undergo remotely controlled shape changes, stiffening, softening and degradation can be produced. The non-invasive nature of light stimulation also enables to trigger such responses post-fabrication, during the maturation phase of a construct. Such unique ability can be used to mimic the dynamic processes occurring in tissue regeneration, as well as in disease progression and degenerative processes in vivo. Bringing together these novel multidisciplinary expertise, the present Special Issue aims to discuss the most recent trends, strategies and novel light-based technologies in the field of biofabrication. These include: 1) using light-based bioprinting to develop in vitro models for drug screening, developmental biology models, disease models, and also functional tissues for implantation; 2) novel light-based biofabrication technologies; 3) development of new photo-responsive bioinks or biomaterial inks.

Published
2023

37. Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Author

Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Paulina N., Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, Lim, Khoon S., Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Paulina N., Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, and Lim, Khoon S.

Abstract

Sacrificial printing allows introduction of architectural cues within engineered tissue constructs. This strategy adopts the use of a 3D-printed sacrificial ink that is embedded within a bulk hydrogel which is subsequently dissolved to leave open-channels. However, current conventional sacrificial inks do not recapitulate the dynamic nature of tissue development, such as the temporal presentation of architectural cues matching cellular requirements during different stages of maturation. To address this limitation, a new class of sacrificial inks is developed that exhibits tailorable and programmable delayed dissolution profiles (1–17 days), by exploiting the unique ability of the ruthenium complex and sodium persulfate initiating system to crosslink native tyrosine groups present in non-chemically modified gelatin. These novel sacrificial inks are also shown to be compatible with a range of biofabrication technologies, including extrusion-based printing, digital-light processing, and volumetric bioprinting. Further embedding these sacrificial templates within cell-laden bulk hydrogels displays precise control over the spatial and temporal introduction of architectural features into cell-laden hydrogel constructs. This approach demonstrates the unique capacity of delaying dissolution of sacrificial inks to modulate cell behavior, improving the deposition of mineralized matrix and capillary-like network formation in osteogenic and vasculogenic culture, respectively.

Published
2023

38. Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Author

Equine Musculoskeletal Biology, CS_Locomotion, Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Paulina N., Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, Lim, Khoon S., Equine Musculoskeletal Biology, CS_Locomotion, Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Paulina N., Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, and Lim, Khoon S.

Published
2023

39. Harnessing light in biofabrication

Author

Equine Musculoskeletal Biology, CS_Locomotion, Levato, Riccardo, Lim, Khoon S., Equine Musculoskeletal Biology, CS_Locomotion, Levato, Riccardo, and Lim, Khoon S.

Published
2023

40. Harnessing light in biofabrication

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), MKA/BT Onderzoek, Levato, Riccardo, Lim, Khoon S., Orthopaedie Onderzoek, Other research (not in main researchprogram), MKA/BT Onderzoek, Levato, Riccardo, and Lim, Khoon S.

Published
2023

41. Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Author

MKA/BT Onderzoek, Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, Lim, Khoon S., MKA/BT Onderzoek, Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Soliman, Bram G., Longoni, Alessia, Wang, Mian, Li, Wanlu, Bernal, Cianciosi, Alessandro, Lindberg, Gabriella C.J., Malda, Jos, Groll, Juergen, Jungst, Tomasz, Levato, Riccardo, Rnjak-Kovacina, Jelena, Woodfield, Tim B.F., Zhang, Yu Shrike, and Lim, Khoon S.

Published
2023

43. Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D‐Bioprinted Constructs (Adv. Funct. Mater. 8/2023)

Author

Soliman, Bram G., primary, Longoni, Alessia, additional, Wang, Mian, additional, Li, Wanlu, additional, Bernal, Paulina N., additional, Cianciosi, Alessandro, additional, Lindberg, Gabriella C.J., additional, Malda, Jos, additional, Groll, Juergen, additional, Jungst, Tomasz, additional, Levato, Riccardo, additional, Rnjak‐Kovacina, Jelena, additional, Woodfield, Tim B. F., additional, Zhang, Yu Shrike, additional, and Lim, Khoon S., additional

Published
2023
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