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Inactivated tissue culture adapted Rift Valley Fever (RVF) Virus vaccines were prepared usingMontanide oil ISA 201 VG and aluminum hydroxide gel as adjuvants. The prepared vaccines weresterile and safe inducing no systemic or local clinical signs in sheep. Comparative evaluation ofexperimentally prepared vaccines in sheep after a single dose indicated that the oily prepared vaccinesstimulated the humeral immune response as estimated by SNT and ELISA compared with aluminumhydroxide gel vaccine. Protective serum antibody titers against in Montanide oil ISA 201 VGadjuvenated RVF vaccine appeared in protective level at 1st week post vaccination and aluminumhydroxide gel adjuvenated RVF vaccine at 2nd week. This protective titer persisted till 12th month inthe new oily vaccine and till the 6th month for Aluminum hydroxide gel vaccine. Both of SNT andELISA indicated that among the used adjuvant Montanide oil ISA 201 VG was preferable

This work aimed to study the clinical aspects and immune response of pregnant and non-pregnant ewes vaccinated with attenuated MP12 RVF vaccine. Non-pregnant ewes showed slight elevation of body temperature lasted for one day post-vaccination. There were no detectable clinical signs of RVF disease. Pregnant ewes showed no elevation in body temperature, no abortion and delivered healthy lambs (without abnormalities). RVF virus was detected on the 2nd day post-vaccination in 15 out of 18 serum samples using RT- PCR. IgM antibodies were detected on 7 days post-vaccination (DPV) and persisted up to 150 DPV with a titer ranged from 100 – 800. IgG antibodies were detected on 7 DPV reaching the peak on 60 DPV and persisted up to 360 DPV with a titer ranged from 100 – 800. Maternal immunity appeared in newly born lambs after ingestion of colosrum and lasted up to 4 months. It is concluded that MP12 RVF vaccine is safe and potent for vaccination of sheep.