294 results on '"Lienemann, P."'
Search Results
2. LGBTQ Utilization of a Statewide Tobacco Quitline: Engagement and Quitting Behavior, 2010–2022
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Anderson, Christopher M, Tedeschi, Gary J, Cummins, Sharon E, Lienemann, Brianna A, Zhuang, Yue-Lin, Gordon, Bob, Hernández, Sandra, and Zhu, Shu-Hong
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Health Services and Systems ,Health Sciences ,Behavioral and Social Science ,Substance Misuse ,Health Disparities ,Tobacco ,Clinical Research ,Sexual and Gender Minorities (SGM/LGBT*) ,Rural Health ,Social Determinants of Health ,Minority Health ,Tobacco Smoke and Health ,Prevention ,Health Services ,3.1 Primary prevention interventions to modify behaviours or promote wellbeing ,Good Health and Well Being ,Humans ,Male ,Female ,Smoking Cessation ,Sexual and Gender Minorities ,Tobacco Use ,Smoking ,Counseling ,Hotlines ,Tobacco Products ,Clinical Sciences ,Public Health and Health Services ,Marketing ,Public Health ,Epidemiology ,Public health - Abstract
IntroductionLesbian, gay, bisexual, transgender, and queer/questioning (LGBTQ) individuals use tobacco at disproportionately high rates but are as likely as straight tobacco users to want to quit and to use quitlines. Little is known about the demographics and geographic distribution of LGBTQ quitline participants, their engagement with services, or their long-term outcomes.Aims and methodsCalifornians (N = 333 429) who enrolled in a statewide quitline 2010-2022 were asked about their sexual and gender minority (SGM) status and other baseline characteristics. All were offered telephone counseling. A subset (n = 19 431) was followed up at seven months. Data were analyzed in 2023 by SGM status (LGBTQ vs. straight) and county type (rural vs. urban).ResultsOverall, 7.0% of participants were LGBTQ, including 7.4% and 5.4% of urban and rural participants, respectively. LGBTQ participants were younger than straight participants but had similar cigarette consumption. Fewer LGBTQ participants reported a physical health condition (42.1% vs. 48.4%) but more reported a behavioral health condition (71.1% vs. 54.5%; both p's < .001). Among both LGBTQ and straight participants, nearly 9 in 10 chose counseling and both groups completed nearly three sessions on average. The groups had equivalent 30-day abstinence rates (24.5% vs. 23.2%; p = .263). Similar patterns were seen in urban and rural subgroups.ConclusionsLGBTQ tobacco users engaged with and appeared to benefit from a statewide quitline even though it was not LGBTQ community-based. A quitline with staff trained in LGBTQ cultural competence can help address the high prevalence of tobacco use in the LGBTQ community and reach members wherever they live.ImplicationsThis study describes how participants of a statewide tobacco quitline broke down by sexual orientation and gender. It compares participants both by SGM status and by type of county to provide a more complete picture of quitline participation both in urban areas where LGBTQ community-based cessation programs may exist and in rural areas where they generally do not. To our knowledge, it is the first study to compare LGBTQ and straight participants on their use of quitline services and quitting aids, satisfaction with services received, and rates of attempting quitting and achieving prolonged abstinence from smoking.
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- 2024
3. Pixel-Level Explanation of Multiple Instance Learning Models in Biomedical Single Cell Images
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Sadafi, Ario, Adonkina, Oleksandra, Khakzar, Ashkan, Lienemann, Peter, Hehr, Rudolf Matthias, Rueckert, Daniel, Navab, Nassir, and Marr, Carsten
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Electrical Engineering and Systems Science - Image and Video Processing ,Computer Science - Computer Vision and Pattern Recognition ,Computer Science - Machine Learning - Abstract
Explainability is a key requirement for computer-aided diagnosis systems in clinical decision-making. Multiple instance learning with attention pooling provides instance-level explainability, however for many clinical applications a deeper, pixel-level explanation is desirable, but missing so far. In this work, we investigate the use of four attribution methods to explain a multiple instance learning models: GradCAM, Layer-Wise Relevance Propagation (LRP), Information Bottleneck Attribution (IBA), and InputIBA. With this collection of methods, we can derive pixel-level explanations on for the task of diagnosing blood cancer from patients' blood smears. We study two datasets of acute myeloid leukemia with over 100 000 single cell images and observe how each attribution method performs on the multiple instance learning architecture focusing on different properties of the white blood single cells. Additionally, we compare attribution maps with the annotations of a medical expert to see how the model's decision-making differs from the human standard. Our study addresses the challenge of implementing pixel-level explainability in multiple instance learning models and provides insights for clinicians to better understand and trust decisions from computer-aided diagnosis systems., Comment: Accepted for publication at the international conference on Information Processing in Medical Imaging (IPMI 2023)
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- 2023
4. Unsupervised Cross-Domain Feature Extraction for Single Blood Cell Image Classification
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Salehi, Raheleh, Sadafi, Ario, Gruber, Armin, Lienemann, Peter, Navab, Nassir, Albarqouni, Shadi, and Marr, Carsten
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Computer Science - Computer Vision and Pattern Recognition ,Electrical Engineering and Systems Science - Image and Video Processing - Abstract
Diagnosing hematological malignancies requires identification and classification of white blood cells in peripheral blood smears. Domain shifts caused by different lab procedures, staining, illumination, and microscope settings hamper the re-usability of recently developed machine learning methods on data collected from different sites. Here, we propose a cross-domain adapted autoencoder to extract features in an unsupervised manner on three different datasets of single white blood cells scanned from peripheral blood smears. The autoencoder is based on an R-CNN architecture allowing it to focus on the relevant white blood cell and eliminate artifacts in the image. To evaluate the quality of the extracted features we use a simple random forest to classify single cells. We show that thanks to the rich features extracted by the autoencoder trained on only one of the datasets, the random forest classifier performs satisfactorily on the unseen datasets, and outperforms published oracle networks in the cross-domain task. Our results suggest the possibility of employing this unsupervised approach in more complicated diagnosis and prognosis tasks without the need to add expensive expert labels to unseen data., Comment: Accepted for publication at the 25th International Conference on Medical Image Computing and Computer Assisted Intervention - MICCAI 2022
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- 2022
5. California School Staff Reports of Seeing Students Vaping at School and Disciplinary Actions.
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Cole, Adam G, Lienemann, Brianna A, Sun, Joanna, Chang, Jacqueline, and Zhu, Shu-Hong
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Nursing ,Health Sciences ,Clinical Research ,vaping ,school discipline ,adolescent health ,alcohol ,tobacco ,drug use prevention ,alcohol/tobacco/drug use prevention ,Specialist Studies in Education - Abstract
Youth vaping is a concern and schools may use many approaches to discipline students caught vaping at school. This study identified the prevalence of school staff seeing vaping in schools and the measures used to discipline students. A state-wide sample of 7,938 staff from 255 middle and high schools reported whether they saw any students vaping at school in the last 30 days, whether they have caught any students vaping during class in the last semester, and what happened after catching a student vaping in class. Open-text responses were coded and themes were identified related to disciplinary approaches. 31.9% of staff reported seeing students vaping at school, and 11.9% of teachers reported catching a student vaping during class. Teachers described four categories of disciplinary approaches after catching students vaping in class: no consequences, punitive approaches, restorative approaches, and mixed approaches. Additional support is necessary to help schools address student vaping.
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- 2022
6. Comparative analysis of CO2 reduction by soluble Escherichia coli formate dehydrogenase H and its selenocysteine-to-cysteine substitution variant
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Feilong Li, Silvan Scheller, and Michael Lienemann
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Formate dehydrogenase ,Molybdenum ,Selenocysteine ,Carbon dioxide reduction ,Metalloenzyme ,Technology - Abstract
Metal-dependent formate dehydrogenases (Me-FDHs) are highly active CO2-reducing enzymes operating at low redox potentials and employ either molybdenum or tungsten to reduce the bound substrate. This makes them suitable for electrochemical applications such as fossil-free production of commodity chemicals utilizing renewable energy. Electrocatalytic CO2 reduction by cathode-immobilized Me-FDHs has been recently demonstrated and rational protein engineering can be used to optimize Me-FDHs for various carbon reduction reactions. In the present study, CO2 reduction by soluble monomeric Escherichia coli formate dehydrogenase H (EcFDH-H) was demonstrated and the function of its nucleophilic selenocysteine residue as a transient ligand of a centrally bound molybdenum atom was investigated. Kinetic analysis of the wildtype enzyme revealed maximum CO2 reduction rates of 44 ± 6 s−1 at pH 5.8 that was decreased to 19% and 0% in the case of selenocysteine substitution with the structural homologues cysteine and serine, respectively. Further selenocysteine-to-cysteine substitution effects included an increased acid tolerance as well as stronger inhibition by nitrate and azide indicating a shift of the Mo oxidation state from IV to VI. Conversely, a destabilizing effect on the oxidized Mo(VI) center could be assigned to the native selenocysteine residue that may facilitate the observed efficient CO2 reduction by rapid transition between Mo oxidation states. Taken together, the performed characterization of EcFDH-H as a catalyst for CO2 reduction and the selenocysteine substitution analysis furthers the understanding of the active-site structure of Me-FDHs and thereby supports the development of more efficient biocatalysts for CO2 reduction.
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- 2023
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7. American Indian/Alaska Native Smokers' Utilization of a Statewide Tobacco Quitline: Engagement and Quitting Behaviors From 2008 to 2018.
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Lienemann, Brianna A, Cummins, Sharon E, Tedeschi, Gary J, Wong, Shiushing, and Zhu, Shu-Hong
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Prevention ,Behavioral and Social Science ,American Indian or Alaska Native ,Health Services ,Cancer ,Clinical Research ,Good Health and Well Being ,Adolescent ,Adult ,Alaskan Natives ,American Indians or Alaska Natives ,California ,Counseling ,Female ,Health Knowledge ,Attitudes ,Practice ,Hotlines ,Humans ,Male ,Middle Aged ,Motivation ,Random Allocation ,Smokers ,Smoking ,Smoking Cessation ,Time Factors ,Tobacco Use Cessation Devices ,Young Adult ,Clinical Sciences ,Public Health and Health Services ,Marketing ,Public Health - Abstract
IntroductionThe objective of this study was to examine access, engagement, and quitting behaviors of American Indian/Alaska Native (AIAN) callers to the California Smokers' Helpline. Telephone counseling is the primary function of the quitline. The overarching theoretical framework for California's quitline is social cognitive theory, although it also utilizes motivational interviewing and cognitive-behavioral strategies.Aims and methodsAIAN (n = 16 089) and White (n = 173 425) California quitline callers from 2009 to 2018 were compared on their characteristics, engagement, and quitting behaviors. Quitline callers responded to a telephone survey at intake. A random selection was called for evaluation 7 months later (White n = 8194, AIAN n = 764). Data from the 2009 to 2017 California Health Interview Survey (CHIS) were used as a reference point for AIANs (AIAN n = 1373).ResultsThe quitline and CHIS had similar proportions of AIANs (4.6% vs. 4.3%, respectively). AIAN smokers were more likely than White smokers to report physical (53.6% vs. 44.9%) and mental (65.7% vs. 57.8%) health conditions at intake. AIANs were more likely to participate in counseling than White callers (67.1% vs. 65.7%). Among those who received counseling, AIANs had greater odds than White smokers of making a quit attempt (adjusted odds ratio = 1.39 [1.06, 1.81]) and similar odds of quitting for 180 days (adjusted odds ratio = 0.95 [0.69, 1.31]).ConclusionsRates of access, engagement, and quitting suggest that individualized quitline counseling was as effective with AIANs as it was with White smokers. Increasing efforts to refer AIANs to existing state quitlines can help more smokers quit.ImplicationsThis study showed that AIAN smokers were well represented among California quitline callers, even without a targeted campaign. It also found that AIAN smokers engaged in quitline services and were as able to quit as their White counterparts were, even after adjusting for other baseline characteristics. One implication is that public health programs can promote quitlines using broad-based campaigns knowing that they will still motivate AIAN smokers to seek help. Another implication is that a standard, individualized counseling protocol delivered by culturally competent quitline staff can effectively help AIAN smokers to quit.
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- 2021
8. Local and Systemic Inflammation in Finnish Dairy Cows with Digital Dermatitis
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Hertta Pirkkalainen, Aino Riihimäki, Taru Lienemann, Marjukka Anttila, Minna Kujala-Wirth, Päivi Rajala-Schultz, Heli Simojoki, Timo Soveri, and Toomas Orro
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digital dermatitis ,dairy cow ,acute phase protein ,cytokine ,histopathology ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Digital dermatitis is a disease of the digital skin and causes lameness and welfare problems in dairy cattle. This study assessed the local and systemic inflammatory responses of cows with different digital dermatitis lesions and compared macroscopical and histological findings. Cow feet (n = 104) were evaluated macroscopically and skin biopsies histologically. Serum samples were analyzed for acute phase proteins (serum amyloid A and haptoglobin) and pro-inflammatory cytokines (interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha). Cows with macroscopically graded active lesions (p = 0.028) and non-active lesions (p = 0.008) had higher interleukin-1 beta levels in their serum compared to healthy cows. Interleukin-1 beta serum concentrations were also higher (p = 0.042) when comparing lesions with necrosis to lesions without necrosis. There was no difference when other cytokine or acute phase protein concentrations in healthy cows were compared to those in cows with different digital dermatitis lesions. A novel histopathological grading was developed based on the chronicity of the lesions and presence of necrosis and ulceration. The presence and number of spirochetes were graded separately. In the most severe chronic lesions, there was marked epidermal hyperplasia and hyperkeratosis with necrosis, deep ulceration, and suppurative inflammation. Spirochetes were found only in samples from necrotic lesions. This study established that digital dermatitis activates proinflammatory cytokines. However, this did not initiate the release of acute phase proteins from the liver. A histopathological grading that takes into account the age and severity of the lesions and presence of spirochetes was developed to better understand the progression of the disease. It is proposed that necrosis of the skin is a result of ischemic necrosis following reduced blood flow in the dermal papillae due to pressure and shear stress caused by thickened epidermis, and that the spirochetes are secondary invaders following tissue necrosis.
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- 2024
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9. The Association of Trail Features With Self-Report Trail Use by Neighborhood Residents.
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Johansen, Christopher, Reynolds, Kim D, Wolch, Jennifer, Byrne, Jason, Chou, Chih-Ping, Boyle, Sarah, Spruijt-Metz, Donna, Lienemann, Brianna A, Weaver, Susan, and Jerrett, Michael
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Public Health ,Health Sciences ,Life on Land ,Chicago ,Exercise ,Humans ,Los Angeles ,Recreation ,Residence Characteristics ,Self Report ,Walking ,exercise ,city planning ,environmental design ,recreation ,Human Movement and Sports Sciences ,Public Health and Health Services ,Curriculum and Pedagogy ,Allied health and rehabilitation science ,Public health ,Sports science and exercise - Abstract
BackgroundUrban trails are a useful resource to promote physical activity. This study identified features of urban trails that correlated with trail use.MethodsMultiuse urban trails were selected in Chicago, Dallas, and Los Angeles. An audit of each trail was completed using the Systematic Pedestrian and Cyclist Environmental Scan for Trails instrument, identifying built environmental features. A self-report of trail use was obtained from trailside residents (N = 331) living within 1 mile of each trail. Univariate and multivariate Poisson regressions controlled for trail time from home and motivation for physical activity.ResultsPositive associations with the past month's hours on the trail were observed for the presence of distance signs, vegetation height, vegetation maintenance, and trail crowding, and a negative association was observed for the presence of crossings on the trail. Positive associations with dichotomous trail use were observed for the presence of distance signs, vegetation height, and vegetation maintenance, and a negative association was observed for the presence of crossings on the trail.ConclusionsThese correlates should be confirmed in other studies and, if supported, should be considered in the promotion and design of urban trails.
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- 2020
10. Pro-tobacco marketing and anti-tobacco campaigns aimed at vulnerable populations: A review of the literature.
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Cruz, Tess Boley, Rose, Shyanika W, Lienemann, Brianna A, Byron, M Justin, Meissner, Helen I, Baezconde-Garbanati, Lourdes, Huang, Li-Ling, Carroll, Dana M, Soto, Claradina, and Unger, Jennifer B
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anti-tobacco ,campaigns ,marketing ,pro-tobacco ,vulnerable populations ,Clinical Sciences - Abstract
INTRODUCTION:We reviewed research literature on pro-tobacco marketing and anti-tobacco campaigns targeting eight vulnerable populations to determine key findings and research gaps. Results can inform tobacco policy and control efforts and the design of public education campaigns for these groups. METHODS:Five journal databases in medicine, communication, and science, were used to identify 8875 peer-reviewed, original articles in English, published in the period 2004-2018. There were 144 articles that met inclusion criteria on pro-tobacco marketing or anti-tobacco campaigns aimed at eight US groups: women of reproductive age, racial/ethnic minority groups (African American, Hispanic/Latino, Asian/Pacific Islander and American Indian/Alaska Native), Lesbian/Gay/Bisexual/Transgender (LGBT) populations, groups with low socioeconomic status, rural/inner city residents, military/veterans, and people with mental health or medical co-morbidities. We summarized the number of articles for each population, type of tobacco, and pro-tobacco or anti-tobacco focus. Narrative summaries were organized by population and by pro-tobacco or anti-tobacco focus, with key strategies and gaps by group. RESULTS:There were more studies on pro-tobacco marketing rather than anti-tobacco campaigns, and on cigarettes rather than other tobacco products. Major gaps included studies on Asian Americans, American Indian/Alaska Natives, pregnant women, LGBT populations, and those with mental health or medical co-morbidities. Gaps related to tobacco products were found for hookah, snus, and pipe/roll-your-own tobacco in the pro-tobacco studies, and for all products except cigarettes in anti-tobacco studies. Common tobacco industry methods used were tailoring of product and package design and messages that were used to reach and appeal to different sociodemographic groups. Studies varied by research design making it difficult to compare results. CONCLUSIONS:We found major research gaps for specific groups and tobacco products. Public education campaigns need a stronger foundation in empirical studies focused on these populations. Research and practice would benefit from studies that permit comparisons across studies.
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- 2019
11. Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili
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Lienemann, Michael, TerAvest, Michaela A, Pitkänen, Juha‐Pekka, Stuns, Ingmar, Penttilä, Merja, Ajo‐Franklin, Caroline M, and Jäntti, Jussi
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Biological Sciences ,Industrial Biotechnology ,Generic health relevance ,Bioelectric Energy Sources ,Electrodes ,Electrons ,Escherichia coli ,Fimbriae ,Bacterial ,Mannose-Binding Lectin ,Oxidation-Reduction ,Shewanella ,Microbiology ,Industrial biotechnology - Abstract
Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose-binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose-coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.
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- 2018
12. Explainable AI identifies diagnostic cells of genetic AML subtypes.
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Matthias Hehr, Ario Sadafi, Christian Matek, Peter Lienemann, Christian Pohlkamp, Torsten Haferlach, Karsten Spiekermann, and Carsten Marr
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Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Explainable AI is deemed essential for clinical applications as it allows rationalizing model predictions, helping to build trust between clinicians and automated decision support tools. We developed an inherently explainable AI model for the classification of acute myeloid leukemia subtypes from blood smears and found that high-attention cells identified by the model coincide with those labeled as diagnostically relevant by human experts. Based on over 80,000 single white blood cell images from digitized blood smears of 129 patients diagnosed with one of four WHO-defined genetic AML subtypes and 60 healthy controls, we trained SCEMILA, a single-cell based explainable multiple instance learning algorithm. SCEMILA could perfectly discriminate between AML patients and healthy controls and detected the APL subtype with an F1 score of 0.86±0.05 (mean±s.d., 5-fold cross-validation). Analyzing a novel multi-attention module, we confirmed that our algorithm focused with high concordance on the same AML-specific cells as human experts do. Applied to classify single cells, it is able to highlight subtype specific cells and deconvolve the composition of a patient's blood smear without the need of single-cell annotation of the training data. Our large AML genetic subtype dataset is publicly available, and an interactive online tool facilitates the exploration of data and predictions. SCEMILA enables a comparison of algorithmic and expert decision criteria and can present a detailed analysis of individual patient data, paving the way to deploy AI in the routine diagnostics for identifying hematopoietic neoplasms.
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- 2023
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13. Exploring the Elastomer Influence on the Electromechanical Performance of Stretchable Conductors.
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Lienemann, Samuel, Boda, Ulrika, Mohammadi, Mohsen, Tunhe Zhou, Petsagkourakis, Ioannis, Nara Kim, and Tybrandt, Klas
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- 2024
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14. Erysipelothrix rhusiopathiae infection in a captive white-lipped peccary (Tayassu pecari) in Finland.
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Javela, Hanna-Maaria, Lienemann, Taru, Nordgren, Heli, Malkamäki, Sanna, Sainmaa, Sanna, Kyyrö, Jonna, and Airas, Niina
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BACTERIAL cultures ,ANIMAL species ,INFECTION ,GRAM-positive bacteria ,AUTOPSY ,DEAD - Abstract
Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes infections in several animal species, including erysipelas in swine, lambs and turkeys. In October 2022, a captive, 1-year-old white-lipped peccary (Tayassu pecari), kept in a herd of five peccaries in a zoo in Finland, suddenly developed signs of inappetence and reluctance to move. Despite treatment, the peccary was found dead. At necropsy, the main gross finding was severe acute segmental necrotizing enteritis. Several other organs had lesions compatible with acute septicaemia, including petechiae and ecchymoses. Histopathology of the intestine revealed severe acute multifocal necrotizing enteritis with neutrophilic vasculitis, vascular fibrinoid microthrombi and myriad clusters of densely packed, rod-shaped, gram-positive bacteria on the tips of the intestinal villi. Bacterial culture was identified as E. rhusiopathiae by MALDI-TOF mass spectrometry. To our knowledge, this is the first report of a naturally occurring E. rhusiopathiae infection in a captive white-lipped peccary. Our findings suggest that regular vaccination of captive white-lipped peccaries should be taken into consideration in preventing infections due to E. rhusiopathiae. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Tobacco regulatory compliance with STAKE Act age-of-sale signage among licensed tobacco retailers across diverse neighborhoods in Southern California.
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Sussman, Steve, Cruz, Tess Boley, Smiley, Sabrina L, Chou, Chih-Ping, Unger, Jennifer B, Kintz, Natalie, Rodriguez, Yaneth L, Barahona, Rosa, Lienemann, Brianna A, Pentz, Mary Ann, Samet, Jonathan, and Baezconde-Garbanati, Lourdes
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age-of-sale signs ,compliance ,licensed tobacco retailers ,race/ethnicity ,tobacco ,Clinical Research ,Cancer ,Tobacco Smoke and Health ,Tobacco ,Prevention ,Clinical Sciences - Abstract
IntroductionThe California Stop Tobacco Access to Kids Enforcement (STAKE) Act requires licensed tobacco retailers to post minimum age-of-sale signage at the point of sale. This study investigated STAKE Act compliance in licensed tobacco retailers across four racial/ethnic communities in Southern California.MethodsThe sample consisted of 675 licensed tobacco retailers (excluding chain store supermarkets and pharmacies) randomly selected based on zip codes from predominantly non-Hispanic White (n=196), African American (n=193), Hispanic/Latino (n=186), and Korean American (n=100) communities. A protocol for assessing signage was completed at each store by community health workers (promotoras de salud). The law changed from a minimum age of 18 to 21 years (Tobacco 21) during data collection, as of 9 June 2016. Differences in signage compliance were evaluated before and after changes in the State law.ResultsOverall, 45% of the stores were compliant with posting the required age-of-sale signage (which varied in minimum age by date of collection); 14% of stores did not have any store interior age-of-sale signs, and 41% of stores had some type of age-of-sale sign but were not compliant with the STAKE Act (e.g. 29.5% of the stores had non-compliant tobacco industry We Card signs but not STAKE Act signs). Stores observed after the 2016 implementation of Tobacco 21 had significantly lower STAKE Act signage compliance rates (38.6%) compared to stores observed before the change in the State law (70.9%) (z=6.8623, p
- Published
- 2018
16. Tobacco regulatory compliance with STAKE Act age-ofsale signage among licensed tobacco retailers across diverse neighborhoods in Southern California
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Sussman, Steve, Cruz, Tess Boley, Smiley, Sabrina L, Chou, Chih-Ping, Unger, Jennifer B, Kintz, Natalie, Rodriguez, Yaneth L, Barahona, Rosa, Lienemann, Brianna A, Pentz, Mary Ann, Samet, Jonathan, and Baezconde-Garbanati, Lourdes
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Public Health ,Health Sciences ,Tobacco Smoke and Health ,Prevention ,Clinical Research ,Tobacco ,Cancer ,Good Health and Well Being ,tobacco ,age-of-sale signs ,compliance ,licensed tobacco retailers ,race/ethnicity ,Clinical Sciences ,Public health - Abstract
IntroductionThe California Stop Tobacco Access to Kids Enforcement (STAKE) Act requires licensed tobacco retailers to post minimum age-of-sale signage at the point of sale. This study investigated STAKE Act compliance in licensed tobacco retailers across four racial/ethnic communities in Southern California.MethodsThe sample consisted of 675 licensed tobacco retailers (excluding chain store supermarkets and pharmacies) randomly selected based on zip codes from predominantly non-Hispanic White (n=196), African American (n=193), Hispanic/Latino (n=186), and Korean American (n=100) communities. A protocol for assessing signage was completed at each store by community health workers (promotoras de salud). The law changed from a minimum age of 18 to 21 years (Tobacco 21) during data collection, as of 9 June 2016. Differences in signage compliance were evaluated before and after changes in the State law.ResultsOverall, 45% of the stores were compliant with posting the required age-of-sale signage (which varied in minimum age by date of collection); 14% of stores did not have any store interior age-of-sale signs, and 41% of stores had some type of age-of-sale sign but were not compliant with the STAKE Act (e.g. 29.5% of the stores had non-compliant tobacco industry We Card signs but not STAKE Act signs). Stores observed after the 2016 implementation of Tobacco 21 had significantly lower STAKE Act signage compliance rates (38.6%) compared to stores observed before the change in the State law (70.9%) (z=6.8623, p
- Published
- 2018
17. Production of biopolymer precursors beta-alanine and L-lactic acid from CO2 with metabolically versatile Rhodococcus opacus DSM 43205
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Laura Salusjärvi, Leo Ojala, Gopal Peddinti, Michael Lienemann, Paula Jouhten, Juha-Pekka Pitkänen, and Mervi Toivari
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carbon dioxide ,lithoautotrophic ,hydrogen-oxidizing bacteria ,Rhodococcus opacus ,L-lactic acid ,beta-alanine ,Biotechnology ,TP248.13-248.65 - Abstract
Hydrogen oxidizing autotrophic bacteria are promising hosts for conversion of CO2 into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium R. opacus strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L−1 beta-alanine and 742 mg L−1 L-lactic acid, while autotrophic cultivations with CO2, H2, and O2 resulted in the production of 1.8 mg L−1 beta-alanine and 146 mg L−1 L-lactic acid. Beta-alanine was also produced at 345 μg L−1 from CO2 in electrobioreactors, where H2 and O2 were provided by water electrolysis. This work demonstrates that R. opacus DSM 43205 can be engineered to produce chemicals from CO2 and provides a base for its further metabolic engineering.
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- 2022
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18. Molecular mechanisms of electron transfer employed by native proteins and biological-inorganic hybrid systems
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Michael Lienemann
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Electron transfer ,Electron bifurcation ,Redox potential ,Redox protein ,Enzymatic electrosynthesis ,Protein engineering ,Biotechnology ,TP248.13-248.65 - Abstract
Recent advances in enzymatic electrosynthesis of desired chemicals in biological-inorganic hybrid systems has generated interest because it can use renewable energy inputs and employs highly specific catalysts that are active at ambient conditions. However, the development of such innovative processes is currently limited by a deficient understanding of the molecular mechanisms involved in electrode-based electron transfer and biocatalysis. Mechanistic studies of non-electrosynthetic electron transferring proteins have provided a fundamental understanding of the processes that take place during enzymatic electrosynthesis. Thus, they may help explain how redox proteins stringently control the reduction potential of the transferred electron and efficiently transfer it to a specific electron acceptor. The redox sites at which electron donor oxidation and electron acceptor reduction take place are typically located in distant regions of the redox protein complex and are electrically connected by an array of closely spaced cofactors. These groups function as electron relay centers and are shielded from the surrounding environment by the electrically insulating apoporotein. In this matrix, electrons travel via electron tunneling, i.e. hopping between neighboring cofactors, over impressive distances of upto several nanometers and, as in the case of the Shewanella oneidensis Mtr electron conduit, traverse the bacterial cell wall to extracellular electron acceptors such as solid ferrihydrite. Here, the biochemical strategies of protein-based electron transfer are presented in order to provide a basis for future studies on the basis of which a more comprehensive understanding of the structural biology of enzymatic electrosynthesis may be attained.
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- 2021
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19. Solar-Powered Carbon Fixation for Food and Feed Production Using MicroorganismsA Comparative Techno-Economic Analysis
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Marja Nappa, Michael Lienemann, Camilla Tossi, Peter Blomberg, Jussi Jäntti, Ilkka Juhani Tittonen, and Merja Penttilä
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Chemistry ,QD1-999 - Published
- 2020
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20. Profiling of Organic Compounds in Bioethanol Samples of Different Nature and the Related Fractions
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Carlos Sánchez, Sergio Santos, Raquel Sánchez, Charles-Philippe Lienemann, and José-Luis Todolí
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Chemistry ,QD1-999 - Published
- 2020
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21. Elastic conducting polymer composites in thermoelectric modules
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Nara Kim, Samuel Lienemann, Ioannis Petsagkourakis, Desalegn Alemu Mengistie, Seyoung Kee, Thomas Ederth, Viktor Gueskine, Philippe Leclère, Roberto Lazzaroni, Xavier Crispin, and Klas Tybrandt
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Science - Abstract
Though deformable thermoelectric materials are desirable for integrating thermoelectric devices into wearable electronics, typical thermoelectric materials are too brittle for practical application. Here, the authors report a high-performance elastic composite for stretchable thermoelectric modules.
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- 2020
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22. Improving the Writing Performance of Struggling Writers in Second Grade
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Lienemann, Torri Ortiz, Graham, Steve, Leader-Janssen, Beth, and Reid, Robert
- Abstract
An important goal in preventing writing disabilities is to provide effective early instruction to at-risk students to maximize their writing development. This study examined whether or not explicitly teaching six at-risk second-grade writers, including children with disabilities, how to plan and draft stories would improve their story writing as well as their recall of narrative reading material. The self-regulated strategy development model was used to teach these strategies; the impact of this instruction was evaluated via a multiple-baseline design. Instruction had a positive impact on students' writing, as their stories were longer, more complete, and, with the exception of one student, qualitatively better. Instructional effects also transferred to the recall of narrative reading material for four of the six students. These findings were generally maintained over time.
- Published
- 2006
23. Anomalous magnetic anisotropy of the topmost surface layer of Ni(110)
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Busch, Marco, Lienemann, Jens, Potthoff, Michael, and Winter, Helmut
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Condensed Matter - Materials Science - Abstract
The orientation of the magnetization of a Ni(110) surface was investigated using techniques with different probing depths. By making use of electron capture into excited states of fast He atoms, we found that the magnetization of the topmost surface layer is not aligned along the easy axes of Ni. However, for a 50 ML film Fe on Ni(110) we observed the magnetization of the topmost Fe surface layer is along the easy axes of Fe., Comment: 4 pages, 5 figures, PRB rapid (in press)
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- 2011
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24. Adsorption of Nickel Ions on the γ‑Alumina Surface: Competitive Effect of Protons and Its Impact on Concentration Profiles.
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Fayad, Rita, Couenne, Françoise, Sorbier, Loïc, Jolimaitre, Elsa, Lienemann, Charles-Philippe, Galfre, Aurélie, Jallut, Christian, and Tayakout-Fayolle, Mélaz
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- 2024
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25. Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment
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Philipp S. Lienemann, Queralt Vallmajo‐Martin, Panagiota Papageorgiou, Ulrich Blache, Stéphanie Metzger, Anna‐Sofia Kiveliö, Vincent Milleret, Ana Sala, Sylke Hoehnel, Aline Roch, Raphael Reuten, Manuel Koch, Olaia Naveiras, Franz E. Weber, Wilfried Weber, Matthias P. Lutolf, and Martin Ehrbar
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bone healing ,bone morphogenetic proteins ,growth factors ,hydrogels ,progenitor cells ,Science - Abstract
Abstract The treatment of bone defects with recombinant bone morphogenetic protein‐2 (BMP‐2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low‐dose BMP‐2‐based bone regeneration by mobilizing healing‐associated mesenchymal progenitor cells (MPCs). Smart synthetic hydrogels are used to trap and study endogenous MPCs trafficking to bone defects. Hydrogel‐trapped and prospectively isolated MPCs differentiate into multiple lineages in vitro and form bone in vivo. In vitro screenings reveal that platelet‐derived growth factor BB (PDGF‐BB) strongly recruits prospective MPCs making it a promising candidate for the engineering of hydrogels that enrich endogenous MPCs in vivo. However, PDGF‐BB inhibits BMP‐2‐mediated osteogenesis both in vitro and in vivo. In contrast, smart two‐way dynamic release hydrogels with fast‐release of PDGF‐BB and sustained delivery of BMP‐2 beneficially promote the healing of bone defects. Collectively, it is shown that modulating the dynamics of endogenous progenitor cells in vivo by smart synthetic hydrogels significantly improves bone healing and holds great potential for other advanced applications in regenerative medicine.
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- 2020
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26. Genotyping and phylogenetic placement of Bacillus anthracis isolates from Finland, a country with rare anthrax cases
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Taru Lienemann, Wolfgang Beyer, Kirsti Pelkola, Heidi Rossow, Alexandra Rehn, Markus Antwerpen, and Gregor Grass
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Bacillus anthracis ,Finland ,Whole genome sequencing (WGS) ,Comparative genomics ,Single nucleotide polymorphism (SNP) ,Multiple locus variable number of tandem repeat analysis (VNTR, MLVA) ,Microbiology ,QR1-502 - Abstract
Abstract Background Anthrax, the zoonotic disease caused by the gram-positive bacterium Bacillus anthracis, is nowadays rare in northern parts of Europe including Finland and Scandinavia. Only two minor outbreaks of anthrax in 1988 and in 2004 and one sporadic infection in 2008 have been detected in animals in Finland since the 1970’s. Here, we report on two Finnish B. anthracis strains that were isolated from spleen and liver of a diseased calf related to the outbreak in 1988 (strain HKI4363/88) and from a local scrotum and testicle infection of a bull in 2008 (strain BA2968). These infections occurred in two rural Finnish regions, i.e., Ostrobothnia in western Finland and Päijänne Tavastia in southern Finland, respectively. Results The isolates were genetically characterized by PCR-based methods such as multilocus variable number of tandem repeat analysis (MLVA) and whole genome-sequence analysis (WGS). Phylogenetic comparison of the two strains HKI4363/88 and BA2968 by chromosomal single nucleotide polymorphism (SNP) analysis grouped these organisms within their relatives of the minor canonical A-branch canSNP-group A.Br.003/004 (A.Br.V770) or canonical B-branch B.Br.001/002, respectively. Strain HKI4363/88 clustered relatively closely with other members of the A.Br.003/004 lineage from Europe, South Africa, and South America. In contrast, strain BA2968 clearly constituted a new sublineage within B.Br.001/002 with its closest relative being HYO01 from South Korea. Conclusions Our results suggest that Finland harbors both unique (autochthonous) and more widely distributed, common clades of B. anthracis. We suspect that members of the common clades such as strains HKI4363/88 have been introduced only recently by anthropogenic activities involving importation of contaminated animal products. On the other hand, autochthonous strains such as isolate BA2968 probably have an older history of their introduction into Finland as evidenced by a high number of single nucleotide variant sites in their genomes.
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- 2018
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27. Whole genome sequencing of multidrug-resistant Salmonella enterica serovar Typhimurium isolated from humans and poultry in Burkina Faso
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Assèta Kagambèga, Taru Lienemann, Jonathan G. Frye, Nicolas Barro, and Kaisa Haukka
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MDR Salmonella Typhimurium ,WGS ,Human feces ,Poultry feces ,Burkina Faso ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Abstract Background Multidrug-resistant Salmonella is an important cause of morbidity and mortality in developing countries. The aim of this study was to characterize and compare multidrug-resistant Salmonella enterica serovar Typhimurium isolates from patients and poultry feces. Methods Salmonella strains were isolated from poultry and patients using standard bacteriological methods described in previous studies. The strains were serotype according to Kaufmann-White scheme and tested for antibiotic susceptibility to 12 different antimicrobial agents using the disk diffusion method. The whole genome of the S. Typhimurium isolates was analyzed using Illumina technology and compared with 20 isolates of S. Typhimurium for which the ST has been deposited in a global MLST database.The ResFinder Web server was used to find the antibiotic resistance genes from whole genome sequencing (WGS) data. For comparative genomics, publicly available complete and draft genomes of different S. Typhimurium laboratory-adapted strains were downloaded from GenBank. Results All the tested Salmonella serotype Typhimurium were multiresistant to five commonly used antibiotics (ampicillin, chloramphenicol, streptomycin, sulfonamide, and trimethoprim). The multilocus sequence type ST313 was detected from all the strains. Our sequences were very similar to S. Typhimurium ST313 strain D23580 isolated from a patient with invasive non-typhoid Salmonella (NTS) infection in Malawi, also located in sub-Saharan Africa. The use of ResFinder web server on the whole genome of the strains showed a resistance to aminoglycoside associated with carriage of the following resistances genes: strA, strB, and aadA1; resistance to β-lactams associated with carriage of a bla TEM-1B genes; resistance to phenicol associated with carriage of catA1 gene; resistance to sulfonamide associated with carriage of sul1 and sul2 genes; resistance to tetracycline associated with carriage of tet B gene; and resistance to trimethoprim associated to dfrA1 gene for all the isolates. Conclusion The poultry and human isolates were genetically similar showing a potential food safety risk for consumers. Our finding of multidrug-resistant S. Typhimurium ST313 in poultry feces calls for further studies to clarify the potential reservoirs of this emerging pathogen.
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- 2018
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28. Cine-MRI detection of intraabdominal adhesions: correlation with intraoperative findings in 89 consecutive cases
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Lang, Reinhold, Buhmann, Sonja, Hopman, Alexander, Steitz, Heinrich-Otto, Lienemann, Andreas, Reiser, Maximilian, Jauch, Karl-Walter, and Hüttl, Thomas
- Abstract
Abstract: Background: This study aimed to evaluate the accuracy of functional cine-MRI in detecting abdominal adhesions. Methods: For this study, 89 consecutive patients with adhesion-related complaints after previous abdominal surgery underwent preoperative workup including cine-MRI in transverse and sagittal orientations for a dynamic examination of an induced visceral slide. An abdominal map consisting of nine segments was created to document the location and extent of the adhesion. Cine-MRI and intraoperative findings were correlated. Results: A total of 59 laparotomies and 30 laparoscopies were performed. Four cases required open surgery due to severe adhesions. The use of cine-MRI scan for the detection of adhesions showed an overall accuracy of 90%, a sensitivity of 93%, and a positive predictive value of 96%. The stronger the adhesions, the more accurate the scan findings. Of 44 patients with second-degree MRI scan findings, 50% had second-degree intraoperative findings. Of 35 patients with third- and fourth-degree adhesions on MRI scans, 74% had exactly the same intraabdominal findings at surgery. The MRI scan showed adhesions located in the small intestines (75%), large intestines (35%), abdominal cavity (42%), and reproductive organs (32%). Intraoperatively, adhesions were found in the small intestines (70%), large intestines (40%), abdominal cavity (42%), and reproductive organs (28%). Conclusions: Cine-MRI provides valid preoperative information with respect to extent, location, and strength of intraabdominal adhesions. Cine-MRI is a good alternative for diagnosing abdominal adhesions because objective findings of the scan and intraoperative findings correlate very well with each other.
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- 2024
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29. Elastic conducting polymer composites in thermoelectric modules
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Kim, Nara, Lienemann, Samuel, Petsagkourakis, Ioannis, Alemu Mengistie, Desalegn, Kee, Seyoung, Ederth, Thomas, Gueskine, Viktor, Leclère, Philippe, Lazzaroni, Roberto, Crispin, Xavier, and Tybrandt, Klas
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- 2020
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30. Structural Features of a Full-Length Ubiquitin Ligase Responsible for the Formation of Patches at the Plasma Membrane
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Jan Knop, Tim Lienemann, Haifa El-Kilani, Sven Falke, Catharina Krings, Maria Sindalovskaya, Johannes Bergler, Christian Betzel, and Stefan Hoth
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SAUL1 ,ubiquitin ligase ,U-box ,armadillo repeats ,multi-vesicular bodies ,vesicle transport ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Plant U-box armadillo repeat (PUB-ARM) ubiquitin (Ub) ligases have important functions in plant defense through the ubiquitination of target proteins. Defense against pathogens involves vesicle trafficking and the formation of extracellular vesicles. The PUB-ARM protein SENESCENCE ASSOCIATED UBIQUITIN E3 LIGASE1 (SAUL1) can form patches at the plasma membrane related to tethering multi-vesicular bodies (MVBs) to the plasma membrane. We uncovered the structure of a full-length plant ubiquitin ligase and the structural requirements of SAUL1, which are crucial for its function in patch formation. We resolved the structure of SAUL1 monomers by small-angle X-ray scattering (SAXS). The SAUL1 model showed that SAUL1 consists of two domains: a domain containing the N-terminal U-box and armadillo (ARM) repeats and the C-terminal ARM repeat domain, which includes a positively charged groove. We showed that all C-terminal ARM repeats are essential for patch formation and that this function requires arginine residue at position 736. By applying SAXS to polydisperse SAUL1 systems, the oligomerization of SAUL1 is detectable, with SAUL1 tetramers being the most prominent oligomers at higher concentrations. The oligomerization domain consists of the N-terminal U-box and some N-terminal ARM repeats. Deleting the U-box resulted in the promotion of the SAUL1 tethering function. Our findings indicate that structural changes in SAUL1 may be fundamental to its function in forming patches at the plasma membrane.
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- 2021
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31. Hydrophobin Bilayer as Water Impermeable Protein Membrane.
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Nolle, Friederike, Starke, Leonhard J., Griffo, Alessandra, Lienemann, Michael, Jacobs, Karin, Seemann, Ralf, Fleury, Jean-Baptiste, Hub, Jochen S., and Hähl, Hendrik
- Published
- 2023
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32. Pro-tobacco marketing and anti-tobacco campaigns aimed at vulnerable populations: A review of the literature
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Tess Boley Cruz, Shyanika W. Rose, Brianna A. Lienemann, M. Justin Byron, Helen I. Meissner, Lourdes Baezconde-Garbanati, Li-Ling Huang, Dana M. Carroll, Claradina Soto, and Jennifer B. Unger
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pro-tobacco ,anti-tobacco ,marketing ,campaigns ,vulnerable populations ,Diseases of the respiratory system ,RC705-779 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Introduction We reviewed research literature on pro-tobacco marketing or anti-tobacco campaigns targeting eight vulnerable populations to determine key findings and research gaps. Results can inform tobacco policy and control efforts and the design of public education campaigns for these groups. Methods Five journal databases in medicine, communication, and science, were used to identify 8877 peer-reviewed, original articles in English, published in the period 2004–2018. There were 146 articles that met inclusion criteria on pro-tobacco marketing or anti-tobacco campaigns aimed at eight US groups: women of reproductive age, racial/ethnic minority groups (African American, Hispanic/Latino, Asian/Pacific Islander and American Indian/Alaska Native), Lesbian/Gay/Bisexual/ Transgender (LGBT) populations, groups with low socioeconomic status, rural/inner city residents, military/veterans, and people with mental health or medical co-morbidities. We summarized the number of articles for each population, type of tobacco, and pro-tobacco or anti-tobacco focus. Narrative summaries were organized by population and by pro-tobacco or anti-tobacco focus, with key strategies and gaps by group. Results There were more studies on pro-tobacco marketing rather than anti-tobacco campaigns, and on cigarettes rather than other tobacco products. Major gaps included studies on Asian Americans, American Indian/Alaska Natives, pregnant women, LGBT populations, and those with mental health or medical co-morbidities. Gaps related to tobacco products were found for hookah, snus, and pipe/roll-your-own tobacco in the pro-tobacco studies, and for all products except cigarettes in antitobacco studies. Common tobacco industry methods used were tailoring of product and package design and messages that were used to reach and appeal to different sociodemographic groups. Studies varied by research design making it difficult to compare results. Conclusions We found major research gaps for specific groups and tobacco products. Public education campaigns need a stronger foundation in empirical studies focused on these populations. Research and practice would benefit from studies that permit comparisons across studies.
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- 2019
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33. Mass Spectrometric Analysis of Short-chain Chlorinated Paraffins in Plastic Consumer Products
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Lena Schinkel, Elia Canonica, Peter Lienemann, Davide Bleiner, and Norbert Heeb
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Chlorinated paraffins ,High-resolution mass spectrometry ,Persistent organic pollutants ,Chemistry ,QD1-999 - Published
- 2019
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34. Electrode material studies and cell voltage characteristics of the in situ water electrolysis performed in a pH-neutral electrolyte in bioelectrochemical systems
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Georgy Givirovskiy, Vesa Ruuskanen, Leo S. Ojala, Michael Lienemann, Petteri Kokkonen, and Jero Ahola
- Subjects
Bioengineering ,Electrochemistry ,Materials chemistry ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Hydrogen-oxidizing bacteria (HOB) have been shown to be promising micro-organisms for the reduction of carbon dioxide to a wide range of value-added products in bioelectrochemical systems with in situ water electrolysis of the cultivation medium, also known as a hybrid biological-inorganic systems (HBI). However, scaling up of this process requires overcoming the inherent constraints of the low energy efficiency partly associated with the pH-neutral electrolyte with low conductivity. Most of the research in the field is concentrated on the bacterial cultivation, whereas the analysis and evaluation of the electrode material performance have received little attention in the literature so far. Therefore, in the present work, in situ electrolysis of a pH-neutral medium for HOB cultivation was performed with different combinations of electrode materials. Besides conventional electrode types, electrodes with coatings made of earth-abundant cobalt and a nickel-iron alloy, known for their catalytic activity for the kinetically sluggish oxygen evolution reaction (OER), were prepared and tested as potential substitutes for catalysts made of precious metals. The cultivation of HOB with in situ water electrolysis has been successfully tested in a small scale electrobioreactor in order to support the experimental results. A simplified water electrolysis model was developed and applied to evaluate the current-voltage characteristics of an bioelectrochemical system prototype. Application of the developed model allows quantitative evaluation and comparison of reversible, ohmic, and activation overvoltages of different electrode sets. The modeling results were found to agree well with the experimental data. The developed model and the data gathered can be applied to further investigation, simulation, and optimization of HBI systems.
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- 2019
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35. Environmental Sciences at Universities of Applied Sciences
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Simon Crelier, Olivier Vorlet, Philippe Corvini, and Peter Lienemann
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Ammonia reduction ,Anaerobic digestion ,Biosorption ,Coffee cherry pulp ,Food by-products ,(micro)pollutants ,Chemistry ,QD1-999 - Abstract
Three institutes of the Universities of Applied Sciences that are active in Chemistry and Life Sciences present a selection of their activities in the field of environmental sciences. These projects include analytical monitoring, removal of micropollutants, waste reduction and valorization.
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- 2018
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36. Tobacco regulatory compliance with STAKE Act age-ofsale signage among licensed tobacco retailers across diverse neighborhoods in Southern California
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Steve Sussman, Tess Boley Cruz, Sabrina L. Smiley, Chih-Ping Chou, Jennifer B. Unger, Natalie Kintz, Yaneth L. Rodriguez, Rosa Barahona, Brianna A Lienemann, Mary Ann Pentz, Jonathan Samet, and Lourdes Baezconde-Garbanati
- Subjects
tobacco ,age-of-sale signs ,compliance ,licensed tobacco ,retailers ,race/ethnicity ,Diseases of the respiratory system ,RC705-779 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Introduction The California Stop Tobacco Access to Kids Enforcement (STAKE) Act requires licensed tobacco retailers to post minimum age-of-sale signage at the point of sale. This study investigated STAKE Act compliance in licensed tobacco retailers across four racial/ethnic communities in Southern California. Methods The sample consisted of 675 licensed tobacco retailers (excluding chain store supermarkets and pharmacies) randomly selected based on zip codes from predominantly non-Hispanic White (n=196), African American (n=193), Hispanic/Latino (n=186), and Korean American (n=100) communities. A protocol for assessing signage was completed at each store by community health workers (promotoras de salud). The law changed from a minimum age of 18 to 21 years (Tobacco 21) during data collection, as of 9 June 2016. Differences in signage compliance were evaluated before and after changes in the State law. Results Overall, 45% of the stores were compliant with posting the required age-of-sale signage (which varied in minimum age by date of collection); 14% of stores did not have any store interior age-of-sale signs, and 41% of stores had some type of age-of-sale sign but were not compliant with the STAKE Act (e.g. 29.5% of the stores had non-compliant tobacco industry We Card signs but not STAKE Act signs). Stores observed after the 2016 implementation of Tobacco 21 had significantly lower STAKE Act signage compliance rates (38.6%) compared to stores observed before the change in the State law (70.9%) (z=6.8623, p
- Published
- 2018
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37. Genotyping and phylogenetic placement of Bacillus anthracis isolates from Finland, a country with rare anthrax cases
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Lienemann, Taru, Beyer, Wolfgang, Pelkola, Kirsti, Rossow, Heidi, Rehn, Alexandra, Antwerpen, Markus, and Grass, Gregor
- Published
- 2018
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38. Whole genome sequencing of multidrug-resistant Salmonella enterica serovar Typhimurium isolated from humans and poultry in Burkina Faso
- Author
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Kagambèga, Assèta, Lienemann, Taru, Frye, Jonathan G., Barro, Nicolas, and Haukka, Kaisa
- Published
- 2018
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39. Using Self-Regulated Strategy Development to Improve Expository Writing with Students with Attention Deficit Hyperactivity Disorder
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Lienemann, Torri Ortiz and Reid, Robert
- Abstract
This study investigated the effects of an expository writing strategy implemented using the Self-Regulated Strategy Development model on the writing skills of four, 4th- and 5th-grade students with attention deficit hyperactivity disorder. Instruction had a pronounced positive effect on students' writing. Student essays were longer and more complete. Holistic quality ratings were in the normal range. Findings were generally maintained over time. (Contains 2 tables and 3 figures.)
- Published
- 2008
40. Self-Regulated Strategy Development for Written Expression with Students with Attention Deficit/Hyperactivity Disorder
- Author
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Reid, Robert and Lienemann, Torri Ortiz
- Abstract
Children with attention deficit/hyperactivity disorder (ADHD) are at increased risk of academic difficulties and special education placement (Barkley, 1998). One academic area, written expression, has received little research attention. This study assessed the effectiveness of a validated strategy instruction model--Self-Regulated Strategy Development (SRSD)--on the length, completeness, and holistic quality of narratives of 3 children teacher-identified as struggling young writers, and physician-identified as having ADHD. Results showed marked improvement on all three measures. Implications for future research and practice are discussed. (Contains 2 figures and 1 table.)
- Published
- 2006
41. Self-Regulated Strategy Development for Students with Learning Disabilities
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Lienemann, Torri Ortiz and Reid, Robert
- Abstract
Self-Regulated Strategy Development (SRSD) is a well-established, thoroughly validated instructional model. In this article, we discuss the rationale and steps for implementing it. We also provide practical information for use in teaching and evaluating strategy instruction. This article is intended to guide teacher educators as they help future teachers develop a practical working knowledge of how to effectively implement strategy instruction in the classroom.
- Published
- 2006
42. Strategy Instruction for Students with Learning Disabilities. What Works for Special Needs Learners
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Reid, Robert, Lienemann, Torri Ortiz, Reid, Robert, and Lienemann, Torri Ortiz
- Abstract
Practical and accessible, this book provides the first step-by-step guide to cognitive strategy instruction, which has been shown to be one of the most effective instructional techniques for students with learning problems. Presented are proven strategies that students can use to improve their self-regulated learning, study skills, and performance in specific content areas, including written language, reading, and math. Clear directions for teaching the strategies in the elementary or secondary classroom are accompanied by sample lesson plans and many concrete examples. Enhancing the book's hands-on utility are more than 20 reproducible worksheets and forms. This book contains the following chapters: (1) Why Use Strategy Instruction?; (2) Building Background Knowledge; (3) The Self-Regulated Strategy Development Model; (4) How to Implement the SRSD Model; (5) Self-Regulation Strategy Development Model; (6) Implementing Self-Regulation Strategies; (7) Integrating Strategies and Self-Regulation; (8) Strategies in Written Language; (9) Strategies in Reading Comprehension; (10) Strategies in Mathematics; (11) Study Skills Strategies; and (12) Mnemonics.
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- 2006
43. Tuning the spin-crossover temperature of polynuclear iron(II)–triazole complexes in solution by water and preparation of thermochromic fibers
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Bräunlich, Irene, Lienemann, Samuel, Mair, Christiane, Smith, Paul, and Caseri, Walter
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- 2015
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44. Shiga Toxin–producing Escherichia coli Serotype O78:H in Family, Finland, 2009
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Taru Lienemann, Eeva Salo, Ruska Rimhanen-Finne, Kai Rönnholm, Mari Taimisto, Jari J. Hirvonen, Eveliina Tarkka, Markku Kuusi, and Anja Siitonen
- Subjects
Hemolytic uremic syndrome (HUS) ,cross-contamination ,invasive Shiga toxin- producing E. coli ,STEC ,stx1c ,bacteremia ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Shiga toxin–producing Escherichia coli (STEC) is a pathogen that causes gastroenteritis and bloody diarrhea but can lead to severe disease, such as hemolytic uremic syndrome (HUS). STEC serotype O78:H– is rare among humans, and infections are often asymptomatic. We detected a sorbitol-fermenting STEC O78:H–:stx1c:hlyA in blood and fecal samples of a 2-week-old boy who had bacteremia and HUS and in fecal samples of his asymptomatic family members. The phenotypic and genotypic characteristics and the virulence properties of this invasive STEC were investigated. Our findings demonstrate that contrary to earlier suggestions, STEC under certain conditions can invade the human bloodstream. Moreover, this study highlights the need to implement appropriate diagnostic methods for identifying the whole spectrum of STEC strains associated with HUS.
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- 2012
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45. Source apportionment of size and time resolved trace elements and organic aerosols from an urban courtyard site in Switzerland
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A. Richard, M. F. D. Gianini, C. Mohr, M. Furger, N. Bukowiecki, M. C. Minguillón, P. Lienemann, U. Flechsig, K. Appel, P. F. DeCarlo, M. F. Heringa, R. Chirico, U. Baltensperger, and A. S. H. Prévôt
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Time and size resolved data of trace elements were obtained from measurements with a rotating drum impactor (RDI) and subsequent X-ray fluorescence spectrometry. Trace elements can act as indicators for the identification of sources of particulate matter 10) in ambient air. Receptor modeling was performed with positive matrix factorization (PMF) for trace element data from an urban background site in Zürich, Switzerland. Eight different sources were identified for the three examined size ranges (PM1−0.1, PM2.5−1 and PM10−2.5): secondary sulfate, wood combustion, fire works, road traffic, mineral dust, de-icing salt, industrial and local anthropogenic activities. The major component was secondary sulfate for the smallest size range; the road traffic factor was found in all three size ranges. This trace element analysis is complemented with data from an Aerodyne high-resolution time-of-flight aerosol mass spectrometer (AMS), assessing the PM1 fraction of organic aerosols. A separate PMF analysis revealed three factors related to three of the sources found with the RDI: oxygenated organic aerosol (OOA, related to inorganic secondary sulfate), hydrocarbon-like organic aerosol (HOA, related to road traffic) and biomass burning organic aerosol (BBOA), explaining 60 %, 22 % and 17 % of total measured organics, respectively. Since different compounds are used for the source classification, a higher percentage of the ambient PM10 mass concentration can be apportioned to sources by the combination of both methods.
- Published
- 2011
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46. Quantitative sampling and analysis of trace elements in atmospheric aerosols: impactor characterization and Synchrotron-XRF mass calibration
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A. Richard, N. Bukowiecki, P. Lienemann, M. Furger, M. Fierz, M. C. Minguillón, B. Weideli, R. Figi, U. Flechsig, K. Appel, A. S. H. Prévôt, and U. Baltensperger
- Subjects
Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
Identification of trace elements in ambient air can add substantial information to pollution source apportionment studies, although they do not contribute significantly to emissions in terms of mass. A method for quantitative size and time-resolved trace element evaluation in ambient aerosols with a rotating drum impactor and synchrotron radiation based X-ray fluorescence is presented. The impactor collection efficiency curves and size segregation characteristics were investigated in an experiment with oil and salt particles. Cutoff diameters were determined through the ratio of size distributions measured with two particle sizers. Furthermore, an external calibration technique to empirically link fluorescence intensities to ambient concentrations was developed. Solutions of elemental standards were applied with an ink-jet printer on thin films and area concentrations were subsequently evaluated with external wet chemical methods. These customized and reusable reference standards enable quantification of different data sets analyzed under varying experimental conditions.
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- 2010
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47. Quantitative analysis of heavy metals in automotive brake linings: A comparison between wet-chemistry based analysis and in-situ screening with a handheld X-ray fluorescence spectrometer
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Figi, R., Nagel, O., Tuchschmid, M., Lienemann, P., Gfeller, U., and Bukowiecki, N.
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- 2010
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48. Depression and Cognitive Impairment in Disability-Free Early Multiple Sclerosis
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Claus G. Haase, Michael Tinnefeld, Marc Lienemann, Reinhard E. Ganz, and Pedro M. Faustmann
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Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Cognitive and emotional capabilities were evaluated in 73 female patients with stable relapsing-remitting definite, and/or laboratory-supported multiple sclerosis (MS) and were compared with 32 matched healthy controls. Patients were categorized according to their score in the expanded disability status scale (EDSS) to either no (EDSS 0, n = 33) or few clinical signs (EDSS 1–2, n = 40) of MS without physical disability. Patients with EDSS > 0 were characterized by significantly (p These results indicate that depression may present as an early sign in MS followed by cognitive impairment, in particular visuo-spatial short-term memory, before physical disability appears. Neuropsychological tests as mentioned here could serve as early diagnostic tools to detect subtle disease progression and to initiate and monitor disease modifying therapies.
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- 2003
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49. Functional interactions between the fossa ischioanalis, levator ani and gluteus maximus muscles of the female pelvic floor: a prospective study in nulliparous women
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Soljanik, Irina, Janssen, Udo, May, Florian, Fritsch, Helga, Stief, Christian G., Weissenbacher, Ernst R., Friese, Klaus, and Lienemann, Andreas
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- 2012
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50. Insight into the micro scale dynamics of a micro fluidic wetting-based conveying system by particle based simulation
- Author
-
Lienemann, Jan, Weiß, Dennis, Greiner, Andreas, Kauzlaric, David, Grünert, Oliver, and Korvink, Jan G.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
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