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2. Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Author

Sudarma Dita Wijayanti, Lidiia Tsvik, and Dietmar Haltrich

Subjects
enzyme, electrochemical biosensor, food analysis, amperometry, Chemical technology, TP1-1185
Abstract

Food analysis and control are crucial aspects in food research and production in order to ensure quality and safety of food products. Electrochemical biosensors based on enzymes as the bioreceptors are emerging as promising tools for food analysis because of their high selectivity and sensitivity, short analysis time, and high-cost effectiveness in comparison to conventional methods. This review provides the readers with an overview of various electrochemical enzyme-based biosensors in food analysis, focusing on enzymes used for different applications in the analysis of sugars, alcohols, amino acids and amines, and organic acids, as well as mycotoxins and chemical contaminants. In addition, strategies to improve the performance of enzyme-based biosensors that have been reported over the last five years will be discussed. The challenges and future outlooks for the food sector are also presented.

Published
2023
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3. Flavin Mononucleotide-Dependent <scp>l</scp>-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for <scp>l</scp>-Lactate Biosensors

Author

Lidiia Tsvik, Beate Steiner, Peter Herzog, Dietmar Haltrich, and Leander Sützl

Subjects
General Chemical Engineering, General Chemistry
Abstract

The development of L-lactate biosensors has been hampered in recent years by the lack of availability and knowledge about a wider range and diversity of L-lactate-oxidizing enzymes that can be used as bioelements in these sensors. For decades, L-lactate oxidase of

Published
2022
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4. Rapid measurement of lactate in exhaled breath condensate: biosensor optimisation and in-human proof-of-concept

Author

Shulin Zhang, Yu-Chih Chen, Alaa Riezk, Damien Ming, Lidiia Tsvik, Leander Sützl, Alison Holmes, and Danny O’Hare

Subjects
Fluid Flow and Transfer Processes, Breath Tests, Process Chemistry and Technology, Humans, Bioengineering, Lactic Acid, Biosensing Techniques, Instrumentation, Mass Spectrometry
Abstract

Lactate concentration is of increasing interest as a diagnostic for sepsis, septic shock, and trauma. Compared with the traditional blood sample media, the exhaled breath condensate (EBC) has the advantages of non-invasiveness and higher user acceptance. An amperometric biosensor was developed and its application in EBC lactate detection was investigated in this paper. The sensor was modified with PEDOT:PSS-PB, and two different lactate oxidases (LODs). A rotating disk electrode and Koutecky-Levich analysis were applied for the kinetics analysis and gel optimization. The optimized gel formulation was then tested on disposable screen-printed sensors. The disposable sensors exhibited good performance and presented a high stability for both LOD modifications. Finally, human EBC analysis was conducted from a healthy subject at rest and after 30 min of intense aerobic cycling exercise. The sensor coulometric measurements showed good agreement with fluorometric and triple quadrupole liquid chromatography mass spectrometry reference methods. The EBC lactate concentration increased from 22.5 μM (at rest) to 28.0 μM (after 30 min of cycling) and dropped back to 5.3 μM after 60 min of rest.

Published
2022

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