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19 results on '"Lidiane Lindinalva Barbosa Amorim"'

1. Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Author

Lidiane Lindinalva Barbosa Amorim, José Ribamar Costa Ferreira-Neto, João Pacífico Bezerra-Neto, Valesca Pandolfi, Flávia Tadeu de Araújo, Mitalle Karen da Silva Matos, Mauro Guida Santos, Ederson Akio Kido, and Ana Maria Benko-Iseppon

Subjects
Housekeeping genes, Root tissue, Salt stress, Drought, Phenylpropanoids, Legume, Plant culture, SB1-1110, Biology (General), QH301-705.5
Abstract

Abstract Background Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this species. Real-time quantitative PCR (qPCR) has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. In the present study, nine candidate reference genes were rigorously tested for their application in normalization of qPCR data onto roots of four distinct cowpea accessions under two abiotic stresses: root dehydration and salt (NaCl, 100 mM). In addition, the regulation of four target transcripts, under the same referred conditions was also scrutinized. Results geNorm, NormFinder, BestKeeper, and ΔCt method results indicated a set of three statistically validated RGs for each stress condition: (I) root dehydration (actin, ubiquitin-conjugating enzyme E2 variant 1D, and a Phaseolus vulgaris unknown gene—UNK), and (II) salt (ubiquitin-conjugating enzyme E2 variant 1D, F-box protein, and UNK). The expression profile of the target transcripts suggests that flavonoids are important players in the cowpea response to the abiotic stresses analyzed, since chalcone isomerase and chalcone synthase were up-regulated in the tolerant and sensitive accessions. A lipid transfer protein also participates in the cowpea tolerance mechanisms to root dehydration and salt stress. The referred transcript was up-regulated in the two tolerant accessions and presented no differential expression in the sensitive counterparts. Chitinase B, in turn, generally related to plant defense, was an important target transcript under salt stress, being up-regulated at the tolerant, and down-regulated in the sensitive accession. Conclusions Reference genes suitable for qPCR analyses in cowpea under root dehydration and salt stress were identified. This action will lead to a more accurate and reliable analysis of gene expression on this species. Additionally, the results obtained in this study may guide future research on gene expression in cowpea under other abiotic stress types that impose osmotic imbalance. The target genes analyzed, in turn, deserve functional evaluation due to their transcriptional regulation under stresses and biotechnological potential.

Published
2018
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2. Antimicrobial and Cytotoxic Properties of Extracts from Plants Traditionally Used in North-East Brazil

Author

Luisa Zupin, Alessandro Tossi, Ricardo Salas Roldan Filho, Cláudia Sampaio de Andrade Lima, Airin Aschrafi, Ana Maria Benko-Iseppon, Lidiane Lindinalva Barbosa Amorim, Lívia Maria Batista Vilela, Sergio Crovella, Carlos André dos Santos Silva, Vincenzo Petix, and Lucas Matos de Lima

Subjects
010404 medicinal & biomolecular chemistry, 0303 health sciences, 03 medical and health sciences, Traditional medicine, Cytotoxic T cell, North east, Biology, Antimicrobial, 01 natural sciences, 030304 developmental biology, 0104 chemical sciences
Abstract

The north-eastern region of Brazil is home to communities of diverse ethnic origins that still depend significantly on folk medicine, with emphasis on plant extracts. The present work aims at investigating medicinal plants used in the traditional medicine of north-eastern Brazil with an assessment of the antimicrobial profile of their extracts. The antimicrobial activity of 12 extracts from 11 plant species from eight higher plant families traditionally used was evaluated against three human pathogens (Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae) using the Agar Disk Diffusion and Broth Microdilution assays. The cytotoxic effects were evaluated on human keratinocytes. A hydroalcoholic extract from Anadenanthera colubrina presented the most promising in vitro antimicrobial activity against S.aureus associated with low cytotoxicity towards human keratinocytes. The information gathered in the present study represents a starting point for further research aiming at providing scientific evidence to the empirical usage of medicinal plants in traditional practices.

Published
2021
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3. Estudo comparativo entre ovitrampa e o método LIRAa para avaliação da presença de Aedes aegypti (Diptera:Culicidae) em Pedro II, Piauí, Brasil

Author

Rayane Maria Mendes Monteiro, Lidiane Lindinalva Barbosa Amorim, Thalia Maria de Sousa Soares, Edson Peres dos Santos, Maria Herlândia de Sousa, Liliane Barbosa Amorim, Caio Alves de Oliveira, and Almir Orsano dos Santos

Subjects
Marketing, Pharmacology, Organizational Behavior and Human Resource Management, biology, Strategy and Management, Drug Discovery, Pharmaceutical Science, Aedes aegypti, biology.organism_classification, Humanities
Abstract

O aumento no numero de casos de dengue, Chikungunya e Zika virus se deve a varios fatores, como aumento da urbanizacao, abastecimento de agua irregular e controle ineficaz do vetor primario Aedes aegypti . Assim, estrategias de monitoramento desses vetores sao necessarias para reducao da circulacao viral. O objetivo desse estudo foi avaliar comparativamente, o Levantamento Rapido do indice de Infestacao por Ae . aegypti (LIRAa) e a ovoposicao em armadilhas (ovitrampa) na cidade de Pedro II, PI- Brasil em tres bairros: Boa Esperanca, Cristo Rei e Vila Kolping. As ovitrampas foram elaboradas com pote plastico escuro, contendo no seu interior agua, uma palheta e o larvicida Pyriproxifen. No periodo de 2019 e 2020, colocou-se 31 ovitrampas em pontos estrategicos para contagem e identificacao dos ovos presentes na palheta e a partir dos dados obtidos foi possivel o calculo dos Indices de Positividade de Ovitrampa (IPO) e Indices de Densidade de Ovos (IDO). Os dados obtidos foram comparados com o LIRAa realizado em 2019 nos meses de maio, agosto e outubro. Coletou-se 23.704 ovos. No bairro Boa Esperanca foram 9.020 ovos (38,1%) com IPO = 68,9% e IDO = 222,8. No Cristo Rei foram 9.659 ovos (40,7%), IPO = 72,4% e IDO = 109,6. No Vila Kolping, 5.025 ovos (21,2%), IPO = 47,4% e IDO = 81,3. Os resultados mostraram que o IPO variou muito (27,2% a 100%) entre os bairros amostrados, o que demonstra a grande flutuabilidade populacional do vetor. De acordo com o LIRAa, os bairros ofereceram risco alto de infestacao do vetor, especialmente no mes de maio. No entanto, no mes de outubro os indices aferidos pelo LIRAa tiveram o valor entre 0,8% e 1,3% e os ovos ainda estavam com a densidade alta, como e o caso do bairro Boa Esperanca com 203,6 de IDO. O grupo de depositos que predominou foi o A2, caracterizado por grandes reservatorios de agua, vasos/frascos com agua, pratos, garrafas e outros. Os resultados da pesquisa indicam que o monitoramento por ovitrampas e o metodo LIRAa sao atividades complementares e instrumentos de grande utilidade para a vigilância vetorial, permitindo maior precisao na tomada de decisoes.

Published
2020
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4. A concepção dos pais sobre o atendimento educacional especializado em salas de recursos multifuncionais: uma pesquisa em Oeiras-PI

Author

José Francisco da Silva Filho, Nereyda Áurea de Carvalho Santos, Helena Batista da Costa Dantas, Elaine Carininy Lopes da Costa, Christianne Lopes Costa, and Lidiane Lindinalva Barbosa Amorim

Subjects
Marketing, Pharmacology, Organizational Behavior and Human Resource Management, Strategy and Management, Drug Discovery, Pharmaceutical Science
Published
2020
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5. Os efeitos da capacitação da equipe de enfermagem sobre avaliação e cuidado de pacientes com feridas

Author

Lanielle de Sousa Brito Oliveira, Elaine Carininy Lopes da Costa, Jucileide Gomes Matias, and Lidiane Lindinalva Barbosa Amorim

Subjects
Marketing, Pharmacology, Organizational Behavior and Human Resource Management, business.industry, Strategy and Management, Drug Discovery, Pharmaceutical Science, Medicine, business, Team training, Humanities
Abstract

Cuidar de feridas requer conhecimento sobre as coberturas disponiveis no mercado e as suas indicacoes, sendo fundamental que a equipe de enfermagem se mantenha atualizada para executar o curativo com qualidade e eficiencia. Este trabalho tem como objetivo avaliar o conhecimento da equipe de enfermagem com relacao ao tratamento de feridas em dois hospitais publicos no estado do Piaui, antes e depois de uma atividade de capacitacao profissional. A pesquisa foi realizada em tres fases: antes da intervencao, os participantes responderam um questionario especifico para identificar seus conhecimentos teoricos sobre tratamento de feridas. Na segunda fase, foi realizada uma atividade educativa com carga horaria de 4 horas, com dois grupos de equipes de enfermagem, com conteudo teorico e pratico. Na terceira fase, o conhecimento dos participantes foi reavaliado, apos a capacitacao. As questoes eram relativas a caracterizacao do entrevistado, local de trabalho, aspectos da formacao, atualizacao e tempo de atuacao. Seguido por perguntas sobre conhecimento especifico no tratamento de feridas. A maioria dos sujeitos do estudo tinha idade entre 31 e 40 anos (46%) e era do sexo feminino (86,5%) e apenas 11% realizaram curso de capacitacao em feridas. Antes da intervencao, questoes relacionadas ao tratamento de feridas foram respondidas corretamente apenas 67,5% dos participantes, e apos a intervencao, por 90,8% deles. Constatou-se baixo conhecimento nos parâmetros: desbridamento, exsudato, biofilme e sinais de infeccao, o que deve nortear estrategias para capacitacao das equipes de enfermagem que irao atuar no cuidado direto ao paciente com ferida complexas.

Published
2020
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6. Plant Aquaporins: Diversity, Evolution and Biotechnological Applications

Author

João Pacífico Bezerra-Neto, Valesca Pandolfi, José Miguel Ortega, José Ribamar Costa Ferreira-Neto, Flávia Figueira Aburjaile, Roberta Lane de Oliveira Silva, Ana Maria Benko-Iseppon, Flávia Czekalski de Araújo, Ederson Akio Kido, Lidiane Lindinalva Barbosa Amorim, Manassés Daniel da Silva, and Tetsu Sakamoto

Subjects
0106 biological sciences, Protein family, In silico, Aquaporin, Computational biology, Aquaporins, 01 natural sciences, Biochemistry, Cell wall, 03 medical and health sciences, Molecular Biology, Phylogeny, Plant Proteins, 030304 developmental biology, 0303 health sciences, Chemistry, fungi, Cell Biology, General Medicine, Plants, Membrane, Membrane protein, Cytoplasm, Function (biology), Biotechnology, 010606 plant biology & botany
Abstract

The plasma membrane forms a permeable barrier that separates the cytoplasm from the external environment, defining the physical and chemical limits in each cell in all organisms. The movement of molecules and ions into and out of cells is controlled by the plasma membrane as a critical process for cell stability and survival, maintaining essential differences between the composition of the extracellular fluid and the cytosol. In this process aquaporins (AQPs) figure as important actors, comprising highly conserved membrane proteins that carry water, glycerol and other hydrophilic molecules through biomembranes, including the cell wall and membranes of cytoplasmic organelles. While mammals have 15 types of AQPs described so far (displaying 18 paralogs), a single plant species can present more than 120 isoforms, providing transport of different types of solutes. Such aquaporins may be present in the whole plant or can be associated with different tissues or situations, including biotic and especially abiotic stresses, such as drought, salinity or tolerance to soils rich in heavy metals, for instance. The present review addresses several aspects of plant aquaporins, from their structure, classification, and function, to in silico methodologies for their analysis and identification in transcriptomes and genomes. Aspects of evolution and diversification of AQPs (with a focus on plants) are approached for the first time with the aid of the LCA (Last Common Ancestor) analysis. Finally, the main practical applications involving the use of AQPs are discussed, including patents and future perspectives involving this important protein family.

Published
2019
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7. Cenostigma pyramidale: Ethnomedicinal Properties and Perspectives on A Legume Tree Highly Adapted to Semiarid ‘Caatinga’ Region

Author

Wilson Dias de Oliveira, Valesca Pandolfi, José Ribamar Costa Ferreira-Neto, Lidiane Lindinalva Barbosa Amorim, Ana Maria Benko-Iseppon, Carlos André dos Santos-Silva, Lívia Maria Batista Vilela, Silvany de Sousa Araújo, and Ricardo Salas Roldan Filho

Subjects
Tree (data structure), biology, Botany, Cenostigma, biology.organism_classification, Legume
Published
2021
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8. The WRKY transcription factor family in cowpea: Genomic characterization and transcriptomic profiling under root dehydration

Author

Mitalle Karen da Silva, Matos, Ana Maria, Benko-Iseppon, João Pacifico, Bezerra-Neto, José Ribamar Costa, Ferreira-Neto, Yu, Wang, Hai, Liu, Valesca, Pandolfi, Lidiane Lindinalva Barbosa, Amorim, Lilia, Willadino, Thialisson Caaci, do Vale Amorim, Ederson Akio, Kido, Rosana Pereira, Vianello, Michael P, Timko, and Ana Christina, Brasileiro-Vidal

Subjects
Gene Expression Profiling, Vigna, Amino Acid Motifs, Chromosome Mapping, Genomics, General Medicine, Plant Roots, Droughts, Alternative Splicing, Protein Domains, Gene Expression Regulation, Plant, Stress, Physiological, Multigene Family, Genetics, RNA-Seq, Promoter Regions, Genetic, Plant Proteins, Transcription Factors
Abstract

Cowpea [Vigna unguiculata (L.) Walp.] is one of the most tolerant legume crops to drought and salt stresses. WRKY transcription factor (TF) family members stand out among plant transcriptional regulators related to abiotic stress tolerance. However, little information is currently available on the expression of the cowpea WRKY gene family (VuWRKY) in response to water deficit. Thus, we analyzed genomic and transcriptomic data from cowpea to identify VuWRKY members and characterize their structure and transcriptional response under root dehydration stress. Ninety-two complete VuWRKY genes were found in the cowpea genome based on their domain characteristics. They were clustered into three groups: I (15 members), II (58), and III (16), while three genes were unclassified. Domain analysis of the encoded proteins identified four major variants of the conserved heptapeptide motif WRKYGQK. In silico analysis of VuWRKY gene promoters identified eight candidate binding motifs of cis-regulatory elements, regulated mainly by six TF families associated with abiotic stress responses. Ninety-seven VuWRKY modulated splicing variants associated with 55 VuWRKY genes were identified via RNA-Seq analysis available at the Cowpea Genomics Consortium (CpGC) database. qPCR analyses showed that 22 genes are induced under root dehydration, with VuWRKY18, 21, and 75 exhibiting the most significant induction levels. Given their central role in activating signal transduction cascades in abiotic stress response, the data provide a foundation for the targeted modification of specific VuWRKY family members to improve drought tolerance in this important climate-resilient legume in the developing world and beyond.

Published
2022
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9. Epidemiologia e Políticas Públicas de Saúde - Volume 1

Author

Adriana Vasconcelos Gomes, Juliana Macêdo Magalhães, Kamilla Pinheiro Saraiva, Erica Pereira Oliveira, Ana Beatriz Mendes Rodrigues, Fernanda Kaline Nogueira dos Santos, Tamires Karen MouraMonte, Juliana Ferreira de Sousa, Sayonnara Ferreira Maia, Adricia Cristine de Souza Alves, Maysa Aiany Dias de Sousa Alves, Beatriz Nayara Muniz de Oliveira, Maria Eduarda Mendes Alves, Maria Laura Pereira Paes, Isabela Silva de Medeiros, Fernanda Carolinne Marinho de Araújo, Maria Eduarda Leite de Queiroz Maciel, Mateus Monteiro Torres, Maria Germana Lopes de Lucêna, Maria Juliana Lopes de Lucêna, Isadora Gomes Cavalcanti Silva, Aline T S de Andrade, Paloma G T Aguiar, Brenda O L Silva, Lauanne R F da Silva, Stefanie B da Silva, Carlos A M, Iran A da Silva, Iris P de Araujo Torres, Kellvin C Maciel, Lucas X da Silva Lima, Fabricio A M Esteves, Amanda Alves de Alencar Ribeiro, Ana Clara dos Santos e Silva Costa, Débora dos Reis Soares, Eukália Pereira da Rocha, Flávia Dayana Ribeiro da Silveira, Lívia Carvalho Pereira, Ana Cláudia Ferreira Yonemoto, Mario Ciro Choptian, Ana Clara Sousa Cruz, Marcelo Batista Suehara, Alisson F S B da Silva, Bertoldo Mendes Araujo Nascimento, Sanny Pinheiro Oliveira, Bruno Luciano C A de Oliveira, Ana Lúcia Moreno Amor, Neila Cristina de Jesus Pino Dos Santos, Daise Pereira Machado Rocha, Glauber Andrade dos Santos, Raoni dos Santos Andrade, Edemilton Ribeiro Santos, Aldery Souza Passos, Érica Santos Bomfim, Wesley Aaraujo de Albuquerque, Jéssica Góes da Silva, Ana Maria do Nascimento Cardoso, Carla Andréa Silva Souza, Lara Pereira Leite Alencar, Maria Lucilândia de Sousa, Lívia Maria dos Santos, Edilmara Tavares Gondim, Gledson Micael da Silva Leite, Tacyla Geyce freire Muniz Januário, Ana Raiane Alencar Tranquilino, Jessyca Moreira Maciel, Sabrina Alaide Amorim Alves, Maria Lúcia Duarte Pereira, Maria do Socorro Vieira Lopes, Edilma Gomes Rocha Cavalcante, Ana Rita Regis Borges, Ana Flávia Pereira da Silva, Gabriela Mendonça Zuntini, Angélica Fátima Bonatti, André Luiz Corrêa, José Dias da Silva, Rochelle Foltram, Antônio Homero Rocha Toledo, André Sousa Rocha, Jocélia Medeiros Ximenes, Mirlly de Souza Ferreira, Antônio Renan Santana, Francisca Gevânia Mourão Campos, Luiz Wescley Fontenele Moura, Ghyslândia Nascimento Oliveira, Anna C F Duarte, Daniele Barbosa de Medeiros, Gabriel Meira Cardoso Pereira, Milla Mariane Freitas Silva, Nathalia Soares Silva, Nicole Assis Valadares Tavares, Nyah Rodrigues Jordão, Natalia Roquette Giachetto, Danielle C H França, Antonio Bruno do Nascimento Rodrigues, Lucas R Uchôa, Thalia M de S Soares, Kayra C Guimarães, Bruna Maria da Silva, Antonia Laiana Araújo da Silva, Caroline R de Almeida, Ana Paula Pereira da Silva, Lucas Ananias, Esterfânia Araujo Barbosa Farias, José M da S Santos, Jéssica Sabrinne Araújo Amaral, Jaina Riberio da Silva, Ana Paula Santos Trindade, Anaalyce Portela Galvão Oliveira, Gabriela Alves de Oliveira, José Italo Silva Nascimento, Beatriz Yuki Maruyama, Salma Ali El Chab Parolin, Bernardo Campos Mascarenhas Aguiar, Georgia de Souza Nascimento Bueno, Graziella Patrício Pereira Garcia, Leandro Pinto Amaral, Mércia Gabriele Menezes Barra, Olívia Maria Souza Mendes, Renata Aparecida Pereira, Caio Augusto de Lima, Marcos Vinicius Teixeira Martins, Gilberto Henrique Nogueira Lages Lopes, Kaio Saramago Mendonça, Caroline Coutinho Horácio Alves, Otavio Augusto Freire Campos, Tatiany Calegari, Stefan Vilges de Oliveira, Carlyne Alves de Sousa, Edilany Aguiar Lima, Francisco Geian Araújo Ferreira, Yara Christian Vasconcelos Monte Farias, Larissa Marcos Machado, Cibele Maria dos Santo, Sara Cordeiro Eloia, Cassandra M A R Barros, Joelson dos Santos Almeida, Eduardo Costa Cordeiro, Maria do Rosário Costa Miranda, Eliana Maria Lazzarotto, Luciana Gonçalves Siqueira, Camila Fachin Ross, Marlise Taminski, Angel Renaro Ferreira Luiz, Élvio L R Vieira, Arnaldo Caldas de França, Juliana A Cardoso, Amanda M F Barbosa, Maria C A Oliveira, Gheisa B Campos, Kairo S M Damasceno, Kayo E S Oliveira, Élvio Luís Ramos Vieira, Viviane Colares, Juliana Andrade Cardoso, Amanda Maria Ferreira Barbosa, Maria da Conceição Andrade de Oliveira, Gheisa Bezerra Campos, Kairo Silvestre Meneses Damasceno, Kayo Emmanoel de Souza Oliveira, Emanuella Esteves Machado, Franceline K de Almeida, Bárbara Sarnaglia Colnaghi, Lara Bastos Machado, Simone K A Duarte, Caio Duarte, Hudson P Pinto, Roberto R Barbosa, Leonardo F Vieira, Emilay Valéria Paiva, Dinah Mileo Santoro, Matheus Guida de Oliveira, Anna Giulia Diniz Resende, Isabella Biaso Ribeiro de Oliveira, Luiz Gustavo Oleriano dos Santos, Sabrina Alauk Ribeiro, Vitórya Brito Oliveira, priscila moraes, Fábio Solon Tajra, Gustavo Araújo Rezende, Fellipe Leonardo Torres Dias, Veronica Perius de Brito, Jardel Harison da Costa Freitas, Débora Clemente Paes, Nycolle Almeida Leite, Maria Eduarda da Costa Serpa, Mariana Sales Bastos, Thalia Alves Chagas Menezes, Odaleia Oliveira Farias, Joyce Kelly Da Silva, Suian Sávia Nunes Santos, Sarah Cardoso de Albuquerque, Vanessa Mirtiany Freire dos Santos, Carla Souza dos Anjos, Jonas Borges dos Santos, Amanda dos Santos Rodrigues, Franciare Vieira Silva, Emanuelly Kesley de Freitas Lima, Marcela Farias dos santos, Ana Cláudia Gomes da Hora Rocha, Jadielson Antonio da Silva, Lucas Kayzan Barbosa da Silva, Ana Caroline Melo dos Santos, Julia Perito Alfredo, Luiza Biondi Warlet, Valentina Gromowski Bortoluzz, Camila Perito Alfredo, Lucas Lerro, Leonardo Lerro, Lana Ferreira Porto Coelho Netto, Luísa Lavall Dill, Luiza Pretto Conzatti, Lavínia B Araújo, Rita Maria Rodrigues Bastos, Lidiane Lindinalva Barbosa Amorim, Brendo Silva Sousa, Carina Uchôa Barbosa, Fábio Geovane Pereira dos Santos, Marcelo Macêdo Monteiro, Alan Pablo Antonio Gonçalves Campelo de Sousa, Jully Érika Viana Brandão, Maria Geovana de Mesquita Lima, Tays de Oliveira Lopes Costa, Denilson Rodrigues de Oliveira, Juliana Maria Martins Pinheiro, Marineide Rodrigues do Amorim, Maria Adriely Cunha Lima, Thaís Francielle Santana Vieira, Yasmim Laila Fragoso Cestari, Laryssa Fialho de Oliveira Sousa, Monalysa da Cruz Nascimento, Isabela de Moura Santos, Camilla Radimack Santos de Souza, Evelen Rouse de Souza Santos, Halley Ferraro Oliveira, Márcia A Fernandes, Cláudia Maria Sousa de Carvalho, Pâmela Q Coêlho, Maria das Graças Mendes Lopes, Karoline Gomes Nunes, Joyce Soares e Silva, Márcia Astrês Fernandes, Samuel Henderson Peirera Lopes, Lucíola Galvão Gondim Corrêa Feitosa, Eliana Campêlo Lago, Fabrício Ibiapina Tapety, Rosa Jordana Carvalho, Mateus Ibiapina Vaz de Sousa Cruz, Letícia Viana dos Santos, Ingrid Lopes Torres, Luana Vitória de Araújo da Silva, Gabriela Pedrosa Carvalho, Rosa J Carvalho, Ana Lívia Castelo Branco de Oliveira, Joyce S e Silva, Nicole Maria Brandim de Mesquita Alencar, Danielle Machado Oliveira de Moura, Nayana Santos Arêa Soares, Janaina Maria dos Santos Francisco de Paula, Sandra Cristina Pillon, Mariana Soares Faria, Tiago Almeida Costa, Larissa Aciole Maciel Teixeira, and Laíse Andrade Oliveira

Published
2020
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10. Epidemiologia e Políticas Públicas de Saúde - Volume 2

Author

Antonio Bruno do Nascimento Rodrigues, Lucas R Uchôa, Thalia M de S Soares, Kayra C Guimarães, Fabricio de Sousa Brandão, Samara Raquel dos Santos Lopes, Caroline R de Almeida, Ana Paula Pereira da Silva, Lucas Ananias, Adam Kelsom Gomes Medeiros, José M da S Santos, Jéssica Sabrinne Araújo Amaral, Ana Maria Ferreira da Silva, Ana Paula Santos Trindade, José Italo Silva Nascimento, Laiza de Oliveira do Carmo, Almir Orsano dos Santos, Eliana Maria Lazzarotto, Ana Carolina Momentti, Fábio Solon Tajra, Breno Coelho Mendes, Bruno Guedes Alcoforado Aguiar, Dorcas Lamounier Costa, Francisca Miriane de Araújo Batista, Rodrigo Aragão da Silva, Julia Perito Alfredo, Laura de Lima Bigolin, Luiza Biondi Warlet, Camila Perito Alfredo, Lucas Lerro, Leonardo Lerro, Lana Ferreira Porto Coelho Netto, Gabriela Vasconcelos de Moura, Nariman Mohamad Abdel Salam Suleiman, Rony Kafer Nobre, Kaique Maximo de Oliveira Carvalho, Pollyana dos Santos Gois, Vitória Santos de Santana, Ana Paula de Oliveira Andrade, Gielson Almeida do Sacramento, Priscila Mendes Grana Oliveira, Kaline Vitória Lima Lira, Álvaro Sepúlveda Carvalho Rocha, Antônio Carlos Guilherme Rocha, Emanuelle Cristine Alves dos Santos, José Samuel Teixeira de Sousa, Natália Carvalho de Sousa Santos, Telma Maria Evangelista de Araújo, Karen Lainy dos Reis Nunes, Suzana Fernandes da Silva, Sara Lúcia Portela do Nascimento, Nataly Ramos Rocha, Vitória Assis Lima, Elayne Silva Maia, Kaio Filipe Nunes Lima, Thalita Boanerges Miranda Cardoso, Lícia de Sousa Gonçalves, Larissa do SS Bastos, Lucas A Ferreira, Alex Ranieri Jerônimo Lima, Leonardo Gomes Cavalieri de Moraes, Thaís de Menezes Ribeiro, Lidiane Lindinalva Barbosa Amorim, Caio alves de Oliveira, Rayane M M Monteiro, Edson Peres dos Santos, Thalia Maria de Sousa Soares, Rubens Francisco Pereira Galvão, Liliane Barbosa Amorim, Antonio Robson Dos Santos, Cleyson Pereira Castro Vieira, Gustavo de Oliveira Borges, João Luis Galvão Freitas, João Lucas Pereira Ferreira, Lucas Costa Lopes, Yago de Oliveira Barros, Nadja Janayra Morais Montes, Giovanna Delcole, Natália de Santana Pinto, Otávio Augusto Barbosa de Oliveira, Rodrigo Ferreira Rodrigues, Rodrigo Ruas Floriano de Toledo, Lyslian J A Moreira, Elaine Eugênio de Oliveira, Nicolas Addor, Melissa Diaz, Márcia A Fernandes, Cleidiane Maria Sales de Brito, Gerarlene Ponte Guimarães Santos, Phellype Kayyaã da Luz, Socorro Adriane de S Meneses Brandão, Rayla Maria Pontes Guimarães Costa, Maria do LF Figueiredo, Márcia Tales de Oliveira Gouveia, João Gabriel Ribeiro dos Santos, Márcia Astrês Fernandes, Sandra Cristina Pillon, Aline Rafael de Sousa Ibiapina, Angela dos Santos Silva, Ingrid Raquel Lima Vieira, Bruna Victoria da Silva Passos, Douglas Vieira de Oliveira, Wellington Macêdo Leite, Diellison Layson dos Santos Lima, Maria Theresa de Alencar Ramsdorf, Catherine Alexia Yoshikawa, João Gabriel da Silva Menezes, Luiz Fernando Benazet de Assunção Pereira, Tayná Boldoni Redígulo, Ana Paula Dossi de Guimarães e Queiroz, Mariana D Pedrão, Mariana O Axer, Joubert M Gonçalves, Letícia L M Amaral, Lucas L Oliveira, Lucas S Salgado, Luiza L Porto, Maria Clara M Morais, Raquel D Esteves, Thiago L Carvalho, Vinícius C Soares, Vinícius M Bossi, Natan L De Macedo, Thainá M Saurini, Victor H F Freitas, Camila S Salgado, Marina Lua Vieira de Abreu Costa, Thaís Santos Martins, Fernando José Guedes da Silva, Jaqueline Carvalho e Silva Sales, Marcoeli Silva de Moura, Marla Ariana Silva, Daiane Moreira Aguiar Bernini, Anderson Esteves Bernini, Vinícius Silva Belo, Regina Consolação dos Santos, Heber Paulino Pena, Juliano Teixeira Moraes, Isabelle Assis Morici, Cíntia Gonçalves Oliveira, Deolane Eustáquia Antunes Vasconcelos, Cristiane Rabelo Lisboa, Matheus Alexandre Bezerra Diassis, Ana Victória Mota Lima, Helenilda de Sousa Araújo, Beatriz Gomes Nobre, Lucas Sampaio Siqueira, Antonia Vitória Gomes Pereira, Gleice Laisla de Oliveira Santos, Vitória da Silva Soares, Vivian Rafaela Almeida Santos, Natalya Wegila Felix da Costa, Ian Alves Meneses, Luiz Fernando Reis Macêdo, Gustavo Cabral Forte, Gisely Torres de Alencar, Nadine Roiz Remaili, Ana Carolina de Mesquita Netto Machado, Andressa Ribeiro da Costa, Clara Araújo Adjuto, Gabriel Antunes Sousa Silva, Karinny Guimarães Couto, Maria Eugenia Mana Garavello, Nicole Nogueira Cardoso, Pedro Augusto Carrijo Nunes, Sabrina Oliveira Carvalho, Yasser Nader Abed, Nycolle Santana dos Santos, LEVI ARAUJO BEZERRA, Mikaelly Nunes Vieira, Luiz Carlos Alves de Souza, Paula Ramalho Marques, Adriana Jardim de Almeida, Aline de Oliveira Felix, Gabriela Martins Pereira, Giulia Del Giudice Figueiredo de Araujo, Lorena Costa Araujo, Rebeca Reginna da Costa Hyppolito, Paulo Cesar de Moura Luz, Rafaella Silva Alcântara, Priscylla Frazão Rodrigues, Raphaela da Penha Lins, Tarcísio Rodrigues da Silva, Cláudia Maria Lins Calheiros, Renata Cury Sander, Risocelly dos Santos Andrade Luz, Risolene dos Santos Andrade, Mychelangela de Assis Brito, Silvana Santiago da Rocha, Ruth Silva Lima da Costa, João Vítor da Silva França, Matilde da Silva Conceição, Christopher Wando da Silva Souza, Nadab Santos Ricardo, Nathalia Oliveira Martins Maia, Célio Clayson Souza de Araújo, Aldemir Victor de Araújo Silva, Nathan Lima de Oliveira, Sabrina Brito Ramos, Luis Felipe da Silva Ferreira, Matheus Pedrosa De Oliveira, Nathalia Cristina Pereira De França, Thalis Ferreira De Souza, Stefânia Rodrigues, Angel Renaro Ferreira Luiz, Carla Moreira, Dirce Marisa Lopes de Lima, Vitória de Oliveira, Tiago Antonio de Souza, Paola Isabel Carrasco Asìn, Ester Gomes Gerheim, Isabel Cristina Gonçalves Leite, Luiz C Ribeiro, Victória Machado Scheibe, Stefanie Flach Zanatta, Bruna Reis Krug, Cristiano De Leon, Ludimila Ferreira Sousa Cruz, Monayra Amaral Medeiros, Ana Flávia Resende Romanielo, Giulia Ugucioni Garbelini, Laíne Ribeiro Antonelli, Daniel Martins Borges, Laura Alves Guimarães, and Barbara Correia Neves Sabino

Published
2020
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11. Bioética e Saúde Pública Volume 2

Author

Lyslian J A Moreira, Fernanda de Abreu Pinto, Nathália Sabrina Santana, Ana Carolina da Silva Lopes, VâniaMaria Alves de Sousa, Katrine B Cavalcanti, Ana K S de Oliveira, Alexandre Ribeiro Cajazeira Ramos, Luis Eduardo Soares dos Santos, Jonnison Lima Ferreira, Karla Heline Pereira de Mesquita, Amanda Karoliny Meneses Resende, Stefânia Araújo Pereira, Vanessa Leal Lira, Cristiana Pacífico Oliveira, Bianca Maria Cardoso de Sousa Vieira, Márcia Beatriz De Sousa Gomes, Vitor Kauê de Melo Alves, Kauan Gustavo de Carvalho, Kelven Rubson Soares Bezerra, Francielly de Sousa Rodrigues, Kássia Monicléia Oliveira Evangelista, Nanielle Silva Barbosa, José Francisco Ribeiro, Naldiana Cerqueira Silva, Carlyne Alves de Sousa, Glícia Maria de Oliveira Damasceno, Edilany Aguiar Lima, Tifane Lorraine Cruz da Ponte, Mayara Braz Seridó de Sousa, Samila Sousa Vasconcelos, Weliton Pereira da Luz, Tatiane Carvalho da Silva Brandão, Karla Joelma Bezerra Cunha, Nelson Jorge Carvalho Batista, Francisca Syonara Moraes Rodrigues, Cora Franklina do Carmo Furtado, Francisco José do Nascimento, Francisca Dulcinalda de Paulo Braga, Andressa Queiroz Marques da Silva, Willyane de Andrade Alvarenga, Márcia A Fernandes, Mateus Ibiapina Vaz de Sousa Cruz, Letícia Viana dos Santos, Ingrid Lopes Torres, Luana Vitória de Araújo da Silva, Gabriela Pedrosa Carvalho, Rosa Jordana Carvalho, Elisângela Pereira Oliveira, Layza kelly de Jesus Silva, Nancy Nay Leite de Araujo L Batista, Gildevan Estrela Dantas, Patrícia Nunes da Fonseca, Maria da Penha Lima Coutinho, Tamiris da Costa Brasileiro, Thayro Andrade Carvalho, Rafael Mondego Fontenele, Karla Mota De Matos, Cintia Regina Silva Pimentel, Nisiane Dos Santos, Willams Araujo Da Costa, Isnara Miranda Santos de Carvalho, Bruno V P Costa, Kelly Silva Gomes, Joseniza dos santos Oliveira, Barbara Haab Carvalho de Souza, Caroliny FerreiraRoza, Caroliny Ferreira Roza, Isadora Lopes Almeida, Mile Cristina de Sousa Brasil, Ana Paula do nascimento Rocha, Ludymilla Freitas de Amorim, Dean Douglas Ferreira de Olivindo, Eva Araújo Lima, Diogo Barbosa da Silva Saraiva, Adriele Dantas do Val Silva, Grace Kelly Lima da Fonseca, Francisco Samuel Andrade de Alencar Campelo, Maria Gabrielle Amorim Mendes Melo, Francisca E de S Borges, Francisco Etevânio de Sousa Borges, Francisco Erivânio de Sousa Borges, João B de C Sousa, Bruna Araújo Gomes, Andressa Santos de Carvalho, Kaliny V dos Santos Alves Pereira, Carina Nunes de Lima, Samara Maria Borges Osório de Andrade, José de Siqueira Amorim, Ludiane Rodrigues Dias Silva, Ana Paula Ribeiro de Almeida, Sarah Maria Osório de Carvalho, Antônia S de J Sousa, Antonio Bruno do Nascimento Rodrigues, Maria A A Borges, Hellen C V Pires, Gustavo de Oliveira Borges, Thalia Maria de Sousa Soares, Jacyara Nery de Oliveira, Anaalyce Portela Galvão Oliveira, Nádia Tamires Silva Matos, Rayane M M Monteiro, Lidiane Lindinalva Barbosa Amorim, Ana Kelline Da S Rodrigues, Raquel Vilanova Araújo, Carmem Valdéia Silva Vieira, Helenilto de Freitas Rodrigues, Lidiana Almeida Costa, Lorena de Sousa Morais, Marciel Sousa Silva, Joabson Araujo de Carvalho, Jullymaria Glenda Soares Alencar, Celbe Patrícia Porfírio Franco, Maria Assunção da Silva Lemos, Jussilene Alves Amorim, Neusa Camilla Cavalcante Andrade Oliveira, Juliane Macedo dos Santos, Suelen Rezende Barbosa, Viviane da Paz Brito, Jamielli Dhamesan Barroso Silva, Laiane Maria Ferreira Gentil, Mirella Raíra Ferreira da Silva Santos, Francisco Elivelton Costa Soares, Edenilson de Sousa, Mireli Sousa de Oliveira, Wendesson Gomes de Alencar, Larissa Cristina Fontenelle, Manoel L da S, Raynnã da S Soares, Jackson Brendo Gomes Dantas, Cássia B Silva, Manuelle Rodrigues da Silva, Gabrielle Soares Batista, Karolynne de Freitas Martins e Silva, Antônio Francisco da Silva Lisboa, Denise C de Sousa, Wagner C Lima, Larissa M F Gonçalves, Glauciany Soares Lopes, Danyele Holanda Da Silva, Juliana Sampaio Melo, Thaysla de Oliveira Sousa, Rafael Radison Coimbra Pereira da silva, Luana rocha Cabral, CIDIANNA EMANUELLY MELO DO NASCIMENTO, Mara J M Costa, Érika Galvão Figuereido, Marcela Araújo Sá Nogueir, Cláudia Maria da Silva Vieira, Juliana do Nascimento Sousa, Maria Clara Santos Fonseca, Hiasmim Oliveira Sousa, Camila de Meneses Caetano Viana, Bianca Anne Mendes de Brito, Lidiane Pereira de Albuquerque, Raniella Borges da Silva, Regina Maria Sousa de Araújo, Thaynara N Souza, Dóris da Silva Vilar, Ana Patrícia da Silva Santos, Maria Madalena dos Santos Araújo, Tatiana Elenice Cordeiro Soares, Natália L Mesquita, Renato K P Carvalho, Francisco R G X, Francisco D dos Santos, Maria R G Ximenes, Francisco W M de Sousa, Layse F Q Vasconcelos, Francisca N da S Sabino, Antonio A A Azevedo, Tiago S Farias, Simone R Quirino, José B P Paz, Verena E S Ferreira, Ana V P G Oliveira, Izabelle M N Albuquerque, Fernanda Pimentel de Oliveira, Márcia Gomes Marinheiro Coelho, Rosendo F de Amorim, Francisca Lucélia Ribeiro de Farias, Maria Raquel da Silva Lima, Benedita Nádia Silva Pereira, Francisco Flávio Muniz Rufino, Francisco Marcelo Sousa Mendes, Henrique Riedel Nunes, Gabrielli Carneiro Oliveira, Andreza Almeida Cardoso, Magda Rogéria Pereira Viana, Flávia Maria Da Costa Cunha, Flaviana Rosa Pereira Costa, Rafaella Conceição Da Silva Ribeiro, Wanderson Primo Moura, Sara Lays Myllena Silva Bento, Thays Chagas Santos, Vânia Maria Alves Sousa, Lays Brunnyeli Santos de Oliveira, Patrícia Nunes da Fonsêca, Ricardo N Couto, Igor Castelo Branco Fontenele Costa, Lara Lins Áfio Ponte, Maria IM Dos Santos, Marina Coelho Feitosa, Maria Eduarda Quadros Bezerra Santana, Aline Franco de Aquino, Vânia Maria Alves de Sousa, Amanda de Oliveira Almeida, Gleicy da Silva Araujo, Daniela dos Santos Mangueira de Almeida, Anna Carolinny Ivo Ferreira, Renata Celestino Nunes, Kassianny Moreira De Sousa, Larissa da Silva Sampaio, Karllenh Ribeiro dos Santos, Kayron Rodrigo Ferreira Cunha, Fabrícia Araújo Prudêncio, Luciane V Santos, Francisco A Da R Coelho, Antonio T De Oliveira, Fátima Correia, Marinelle PL Pagkalis, Weslley A Macedo, Guilherme Barroso, Antônio Diego Costa Bezerra, Ana Carla da Costa Leitão Melo, Francisco Ricael Alexandre Maria, Ingrid Laissa Paiva Mota, Maria Ludmylla Barreto Sousa, Shirlene de Mesquita Viana, and Isabella Lima Barbosa Campelo

Published
2020
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12. Plants Defense-related Cyclic Peptides: Diversity, Structure and Applications

Author

Sergio Crovella, Ana Maria Benko-Iseppon, João Pacífico Bezerra Neto, Sheyla Carla Barbosa da Silva Lima, Lidiane Lindinalva Barbosa Amorim, Valesca Pandolfi, José Ribamar Costa Ferreira Neto, Carla Barbosa da Silva Lima, Sheyla, Maria Benko-Iseppon, Ana, Pacifico Bezerra Neto, Joao, Lindinalva Barbosa Amorim, Lidiane, Ribamar Costa Ferreira Neto, Jose, Crovella, Sergio, and Pandolfi, Valesca

Subjects
0301 basic medicine, Peptide, Context (language use), Cyclotides, Biology, Biochemistry, 03 medical and health sciences, Disulfide bonds, MCoTI-I/II, Protease mediated defense, SFTI, Molecular Biology, Cell Biology, Plant defense against herbivory, chemistry.chemical_classification, Disulfide bond, Cystine knot, food and beverages, General Medicine, Cyclotide, Cyclic peptide, Amino acid, 030104 developmental biology, chemistry
Abstract

Plant growth is prone to several unfavorable factors that may compromise or impair development and survival, including abiotic or biotic stressors. Aiming at defending themselves, plants have developed several strategies to survive and adapt to such adversities. Cyclotides are a family of plant-derived proteins that exhibit a diverse range of biological activities including antimicrobial and insecticidal activities that actively participate in plant defense processes. Three main categories of peptides have been described: (i) Cyclotides (ii) Sunflower Trypsin Inhibitor (SFTI) and (iii) peptides MCoTI-I and II, from Momordica cochinchinensis. They comprise proteins of approximately 30 amino acids, containing a head-to-tail cyclized backbone, with three disulfide bonds configured in a cystine knot topology, therefore bearing greater peptide stability. Given their features and multifunctionality, cyclotides stand out as promising sources for the discovery of new antimicrobial agents. The present review describes cyclotide occurrence, abundance and action in plants, also their and evolution. Considerations regarding their use in the context of biomedical and agronomical sciences uses are also presented.

Published
2017
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13. Transcription factors involved in plant resistance to pathogens

Author

João Pacífico Bezerra Neto, Sergio Crovella, Lidiane Lindinalva Barbosa Amorim, Mauro Guida-Santos, Romulo da Fonseca dos Santos, Ana Maria Benko-Iseppon, Amorim, Lidiane L. B., da Fonseca dos Santos, Romulo, Pacífico Bezerra Neto, Joao, Guida-Santos, Mauro, Crovella, Sergio, and Maria Benko-Iseppon, Ana

Subjects
0106 biological sciences, 0301 basic medicine, Transcription, Genetic, Interaction, Interactions, Plant Immunity, Plant disease resistance, Biology, 01 natural sciences, DNA-binding protein, Biochemistry, 03 medical and health sciences, Biotic stress, Gene Expression Regulation, Plant, Stress, Physiological, Protein Interaction Mapping, Protein Isoforms, Transcription factor, Pathogen, Crosstalk, Molecular Biology, Disease Resistance, Plant Diseases, Plant Proteins, Plant defense genes, Cell Biology, Genetics, Ecology, Arabidopsis Proteins, fungi, food and beverages, General Medicine, Plants, Plants, Genetically Modified, WRKY protein domain, DNA-Binding Proteins, 030104 developmental biology, Basic-Leucine Zipper Transcription Factors, Biotic stre, Host-Pathogen Interactions, Plant defense gene, Signal transduction, 010606 plant biology & botany, Signal Transduction, Transcription Factors
Abstract

Phytopathogenic microorganisms have a significant influence on survival and productivity of several crop plants. Transcription factors (TFs) are important players in the response to biotic stresses, as insect attack and pathogen infection. In face of such adversities many TFs families have been previously reported as differentially expressed in plants as a reaction to bacterial, fungal and viral infection. This review highlights recent progresses in understanding the structure, function, signal regulation and interaction of transcription factors with other proteins in response to pathogens. Hence, we focus on three families of transcription factors: ERF, bZIP and WRKY, due to their abundance, importance and the availability of functionally well-characterized members in response to pathogen attack. Their roles and the possibilities related to the use of this knowledge for engineering pathogen resistance in crop plants are also discussed.

Published
2017

14. Resistance (R) Genes: Applications and Prospects for Plant Biotechnology and Breeding

Author

Ana Maria Benko Iseppon, Manassés Daniel da Silva, Sergio Crovella, Ana Carolina Wanderley-Nogueira, Roberta Lane de Oliveira Silva, Ederson Akio Kido, Lidiane Lindinalva Barbosa Amorim, José Ribamar Costa Ferreira Neto, Valesca Pandolfi, Pandolfi, Valesca, Neto, José Ribamar Costa Ferreira, Silva, Manassés Daniel, Amorim, Lidiane Lindinalva Barbosa, Wanderley-Nogueira, Ana Carolina, de Oliveira Silva, Roberta Lane, Kido, Ederson Akio, Crovella, Sergio, and Iseppon, Ana Maria Benko

Subjects
0301 basic medicine, disease resistance, Protein Serine-Threonine Kinases, Biology, Plant disease resistance, directed mutagenesis, Biochemistry, DNA sequencing, 03 medical and health sciences, Genome editing, Gene Expression Regulation, Plant, Cisgenesis, Protein Isoforms, genetic modification, Plant Immunity, Molecular Biology, Gene, Selection (genetic algorithm), Plant Diseases, Plant Proteins, Gene Editing, Resistance (ecology), Arabidopsis Proteins, business.industry, fungi, food and beverages, Cell Biology, General Medicine, plant pathogen, Plants, Plants, Genetically Modified, Biotechnology, Plant Breeding, 030104 developmental biology, Host-Pathogen Interactions, Mutagenesis, Site-Directed, Identification (biology), CRISPR-Cas Systems, business, Signal Transduction
Abstract

The discovery of novel plant resistance (R) genes (including their homologs and analogs) opened interesting possibilities for controlling plant diseases caused by several pathogens. However, due to environmental pressure and high selection operated by pathogens, several crop plants have lost specificity, broad-spectrum or durability of resistance. On the other hand, the advances in plant genome sequencing and biotechnological approaches, combined with the increasing knowledge on R-genes have provided new insights on their applications for plant genetic breeding, allowing the identification and implementation of novel and efficient strategies that enhance or optimize their use for efficiently controlling plant diseases. The present review focuses on main perspectives of application of R-genes and its co-players for the acquisition of resistance to pathogens in cultivated plants, with emphasis on biotechnological inferences, including transgenesis, cisgenesis, directed mutagenesis and gene editing, with examples of success and challenges to be faced.

Published
2017

15. Plant Elite Squad: First Defense Line and Resistance Genes - Identification, Diversity and Functional Roles

Author

Ederson Akio Kido, Lidiane Lindinalva Barbosa Amorim, Sergio Crovella, Ana Carolina Wanderley-Nogueira, Ana Maria Benko-Iseppon, João Pacífico Bezerra-Neto, Flavia Tadeu de Araujo, Wanderley-Nogueira, Ana Carolina, Bezerra-Neto, João Pacífico, Kido, Ederson Akio, de Araújo, Flávia Tadeu, Amorim, Lidiane Lindinalva Barbosa, Crovella, Sergio, and Benko-Iseppon, Ana Maria

Subjects
0301 basic medicine, Hypersensitive response, PAMPs, Systems biology, Gene Dosage, Plant Immunity, Computational biology, Plant disease resistance, Biology, Biochemistry, 03 medical and health sciences, Gene Expression Regulation, Plant, R-gene, Signal transduction, Protein Isoforms, DAMP, Molecular Biology, Gene, Pathogen, Disease Resistance, Plant Diseases, Plant Proteins, DAMPs, Arabidopsis Proteins, Ecology, Chromosome Mapping, Cell Biology, General Medicine, R gene, PAMP, Ethylenes, Plants, 030104 developmental biology, Avirulence, MAMPs, MAMP, Host-Pathogen Interactions, Identification (biology), Reactive Oxygen Species, Genome, Plant, Signal Transduction
Abstract

Plants exhibit sensitive mechanisms to respond to environmental stresses, presenting some specific and non-specific reactions when attacked by pathogens, including organisms from different classes and complexity, as viroids, viruses, bacteria, fungi and nematodes. A crucial step to define the fate of the plant facing an invading pathogen is the activation of a compatible Resistance (R) gene, the focus of the present review. Different aspects regarding R-genes and their products are discussed, including pathogen recognition mechanisms, signaling and effects on induced and constitutive defense processes, splicing and post transcriptional mechanisms involved. There are still countless challenges to the complete understanding of the mechanisms involving R-genes in plants, in particular those related to the interactions with other genes of the pathogen and of the host itself, their regulation, acting mechanisms at transcriptional and post-transcriptional levels, as well as the influence of other types of stress over their regulation. A magnification of knowledge is expected when considering the novel information from the omics and systems biology.

Published
2017

16. Transcription Factors Involved in Plant Drought Tolerance Regulation

Author

José Ribamar Costa Ferreira Neto, Rômulo da Fonseca do Santos, João Pacífico Bezerra-Neto, Ederson Akio Kido, Lidiane Lindinalva Barbosa Amorim, Ana Maria Benko-Iseppon, and Mitalle Karen da Silva Matos

Subjects
0106 biological sciences, 0301 basic medicine, Small RNA, fungi, Drought tolerance, food and beverages, Computational biology, Biology, biology.organism_classification, 01 natural sciences, Chromatin, Transcriptome, 03 medical and health sciences, 030104 developmental biology, Arabidopsis thaliana, Epigenetics, Transcription factor, Gene, 010606 plant biology & botany
Abstract

Drought stress induces the expression of a significant number of transcription factor (TF) genes that are involved in stress tolerance and response. Nowadays, novel “omics” and next-generation sequencing provide a global picture of drought-stress responses and a comprehensive understanding of TF regulatory networks that modulate this response. Several TFs have been shown to be involved in the regulation of stress-responsive genes under drought condition, including Arabidopsis thaliana, rice (Oryza sativa, Poaceae), soybean (Glycine max, Fabaceae), and other plants. Most data have been obtained from transcriptome analyses, studies using small RNA molecules, chromatin modulation, and genomic DNA modifications. Based on recent advances in genomic technologies, improvements in crop drought-stress tolerance have been attained from marker-assisted breeding and gene transfer. In this chapter, we summarize recent progress on the role of TFs in response to drought in plants. Studies on crops have shown that facing drought requires ET and ABA signaling pathways through selectively interacting with TFs, once again highlighting a close relationship between different signaling pathways referred to in this chapter.

Published
2016
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17. Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers

Author

Maria Betânia M. de Oliveira, Lidiane Lindinalva Barbosa Amorim, Reginaldo de Carvalho, Ana Maria Benko-Iseppon, Luiz J. O. Tavares de Melo, Alberto V. C. Onofre, and Maria Lucília M. da Costa

Subjects
Genetic diversity, business.industry, fungi, Drought tolerance, Dendrogram, food and beverages, General Medicine, Biology, Quantitative trait locus, Biotechnology, Crop, Microsatellite, Cultivar, Genetic variability, business
Abstract

Sugarcane is an important tropical crop, responsible for two thirds of the world sugar production, gaining actually importance as a source of biofuel. Drought tolerance is a very important feature considering the actual climate change scenario throughout the world. This study aimed to determine the genetic diversity between sugarcane varieties with contrasting features under drought. For this purpose, twelve ISSR primers were used to characterize nine sugarcane varieties under cultivation in different countries including selected drought resistant material from Northeast Brazil and two varieties from India as contrasting genotypes. 317 scorable bands were generated, among which 301 comprised polymorphic markers, with an average of 25 polymorphic bands per primer. In the generated dendrogram the accessions were placed in clusters, where cluster A included two varieties from India (Co331 and Co419), and B comprised plants eight Brazilian accessions and a ‘Barbado’ variety. Within this clade, drought tolerant and susceptible varieties were clearly separated. The present evaluation revealed important contrasting parental candidates regarding their drought response, very promising for future mapping approaches aiming the identification of quantitative trait loci (QTLs) associated to drought in sugarcane. The selected primers were used for the first time in sugarcane, representing valuable tools for future evaluations, with emphasis to diversity characterization and genetic mapping.

Published
2011
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18. Low genetic diversity and high differentiation among relict populations of the neotropical gymnosperm Podocarpus sellowii (Klotz.) in the Atlantic Forest

Author

Tiago Esposito, Henrique Batalha-Filho, Lidiane Lindinalva Barbosa Amorim, Andrea Pedrosa-Harand, A. C. B. Sousa, Ana Maria Benko-Iseppon, Liliane G. Dantas, and Leonardo P. Felix

Subjects
Conservation genetics, Population, Population genetics, Plant Science, Forests, Evolution, Molecular, Genetic variation, Genetics, Genetic variability, education, Alleles, Phylogeny, education.field_of_study, Genetic diversity, Polymorphism, Genetic, biology, Geography, Ecology, Genetic Variation, General Medicine, biology.organism_classification, Tracheophyta, Genetics, Population, Genetic Loci, Insect Science, Animal Science and Zoology, Podocarpaceae, Podocarpus sellowii, Microsatellite Repeats
Abstract

Podocarpus sellowii (Podocarpaceae) is one of only a few gymnosperms native to Brazil and the sole species of the genus found in the northeastern region of that country. It has a very restricted distribution in this region, with only three known populations in highland forests (called Brejos de Altitude), which apparently have been isolated from each other since the Pleistocene. Due to this long-term isolation and the fact that these populations have few adult individuals and suffer great anthropogenic pressure, low genetic variability is expected, compromising their long-term viability. The present work assessed the genetic variability and structure of northeastern populations of P. sellowii to investigate the role of Pleistocene glaciations on the genetic relationships between them and to propose strategies for their conservation by analyzing the SSR and ISSR markers of adult and juvenile individuals. Low genetic diversity was found with both markers, associated with a high differentiation of the Brejo de Baturite population in relation to the others—suggesting their isolation at different points in time, probably during the Pleistocene. Actions directed towards increasing the genetic diversity of these populations will be needed, such as planting seedlings with high genetic variability—but the high degrees of differentiation observed between the populations must be taken into account.

Published
2014

19. Prospecção de Genes de Resistência à Seca e à Salinidade em Plantas Nativas e Cultivadas (Exploration of Genes for Resistance to Drought and Salinity in Native and Cultivated Plants)

Author

José Ribamar Costa Ferreira Neto, Mauro Santos, Marccus Alves, Roberta Lane de Oliveira Silva, Nina da Mota Soares-Cavalcanti, Ana Maria Benko-Iseppon, Ederson Akio Kido, Lidiane Lindinalva Barbosa Amorim, Valesca Pandolfi, João Pacífico Bezerra Neto, Hayana Millena de Arruda Azevedo, and Luis Carlos Berlarmino

Subjects
lcsh:GE1-350, Atmospheric Science, Cultivated plant taxonomy, Geography, Planning and Development, Context (language use), Limiting, Native flora, Salinity, Horticulture, Geophysics, Geography, Crop production, Computers in Earth Sciences, lcsh:GB3-5030, lcsh:Physical geography, Gene, lcsh:Environmental sciences, Earth-Surface Processes, General Environmental Science
Abstract

A seca, o calor e a salinidade sao fatores limitantes da lavoura em uma parcela significativa de nosso planeta, locais de clima extremo onde apenas plantas adaptadas sao capazes de sobreviver. Porem, quais sao os genes de vegetais determinantes desta capacidade de sobrevivencia? Com o advento da genomica e da transcriptomica novos mecanismos vem sendo revelados, contrariando alguns pressupostos previamente existentes. O presente trabalho comenta as principais familias genicas associadas a seca e a salinidade em angiospermas, enfatizando o potencial que a flora nativa apresenta para a prospeccao de novos genes capazes de contribuir para a geracao de plantas que – mesmo nao sendo completamente adaptadas ao clima Semiarido, como aquelas nativas desse ambiente – devem apresentar melhor adaptacao a periodos inesperados ou mais longos de seca durante seu cultivo, ou mesmo sob niveis moderados de salinidade. Neste contexto, algumas categorias genicas merecem atencao, a luz dos avancos atualmente existentes na transcriptomica, compreendendo principalmente osmoprotetores, fatores de transcricao, canais de agua, LEA ( Late Embryogenesis Abundant ), proteinas dependentes de ABA (acido abscisico), proteinas de choque termico, proteinas transportadoras, fosfolipases C, proteinas de desintoxicacao, chaperonas e proteinas receptoras. Porem, a maioria dos estudos tem se baseado em plantas-modelo ou plantas amplamente cultivadas, havendo necessidade de estudos em plantas nativas de ambientes extremos, como a Caatinga nordestina, o cerrado e os campos rupestres, os quais certamente abrigam um grande numero de variantes destes genes e provavelmente tambem de novos genes importantes para o melhoramento e a biotecnologia vegetal. Palavras-chave: Estresse abiotico, transcriptoma, biotecnologia, tolerância. Exploration of Genes for Resistance to Drought and Salinity in Native and Cultivated Plants ABSTRACT Drought, heat and salinity are limiting factors of crop production in a significant portion of our planet, extreme regions where only adapted plants are able to survive. But what are the determinant genes of plant survivability? With the advent of genomics and transcriptomics new findings have been revealed, some of them confronting previous assumptions. The present work comments on the major gene families related to drought and salinity in angiosperms, emphasizing the potential of the native flora for the discovery of new genes that may contribute to the generation of plants – maybe not fully adapted to the semi-arid climate as native plants – but presenting a better adaptation to unexpected or longer periods of drought during cultivation, or even moderate levels of salinity. In this context, some gene categories deserve a special attention in the light of the current advances in transcriptomics, comprising osmoprotetors, transcription factors, water channels, LEA (Late embryogenesis abundant), protein-dependent ABA (abscisic acid), heat shock proteins, transport proteins, phospholipase C, detoxification proteins, chaperones and receptor proteins. However, most studies have been based on model plants or plants widely grown, with an existing need to study native plants in extreme environments, such as the Brazilian ";Caatinga”, the “cerrados” and “campos rupestres” which certainly harbor a large number of variants of these genes and probably new genes important for plant breeding and biotechnology. Key-words: Abiotic stress, transcriptomics, biotechnology, tolerance.

Published
2012
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