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327 results on '"Licausi, Francesco"'

17. Structural and biochemical characterization of Arabidopsis alcohol dehydrogenases reveals distinct functional properties but similar redox sensitivity

Author

Meloni, Maria, primary, Rossi, Jacopo, additional, Fanti, Silvia, additional, Carloni, Giacomo, additional, Tedesco, Daniele, additional, Treffon, Patrick, additional, Piccinini, Luca, additional, Falini, Giuseppe, additional, Trost, Paolo, additional, Vierling, Elizabeth, additional, Licausi, Francesco, additional, Giuntoli, Beatrice, additional, Musiani, Francesco, additional, Fermani, Simona, additional, and Zaffagnini, Mirko, additional

Published
2024
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21. Acquisition of hypoxia inducibility by oxygen sensing N-terminal cysteine oxidase in spermatophytes

Author

Weits, Daan A, Zhou, Lina, Giuntoli, Beatrice, Carbonare, Laura Dalle, Iacopino, Sergio, Piccinini, Luca, Lombardi, Lara, Shukla, Vinay, Bui, Liem T, Novi, Giacomo, van Dongen, Joost T, Licausi, Francesco, Weits, Daan A, Zhou, Lina, Giuntoli, Beatrice, Carbonare, Laura Dalle, Iacopino, Sergio, Piccinini, Luca, Lombardi, Lara, Shukla, Vinay, Bui, Liem T, Novi, Giacomo, van Dongen, Joost T, and Licausi, Francesco

Abstract

N-terminal cysteine oxidases (NCOs) use molecular oxygen to oxidise the amino-terminal cysteine of specific proteins, thereby initiating the proteolytic N-degron pathway. To expand the characterisation of the plant family of NCOs (plant cysteine oxidases [PCOs]), we performed a phylogenetic analysis across different taxa in terms of sequence similarity and transcriptional regulation. Based on this survey, we propose a distinction of PCOs into two main groups. A-type PCOs are conserved across all plant species and are generally unaffected at the messenger RNA level by oxygen availability. Instead, B-type PCOs appeared in spermatophytes to acquire transcriptional regulation in response to hypoxia. The inactivation of two A-type PCOs in Arabidopsis thaliana, PCO4 and PCO5, is sufficient to activate the anaerobic response in young seedlings, whereas the additional removal of B-type PCOs leads to a stronger induction of anaerobic genes and impairs plant growth and development. Our results show that both PCO types are required to regulate the anaerobic response in angiosperms. Therefore, while it is possible to distinguish two clades within the PCO family, we conclude that they all contribute to restrain the anaerobic transcriptional programme in normoxic conditions and together generate a molecular switch to toggle the hypoxic response.

Published
2023

22. Acquisition of hypoxia inducibility by oxygen sensing N-terminal cysteine oxidase in spermatophytes

Author

Sub Plant-Environment Signaling, Plant-Environment Signaling, Weits, Daan A, Zhou, Lina, Giuntoli, Beatrice, Carbonare, Laura Dalle, Iacopino, Sergio, Piccinini, Luca, Lombardi, Lara, Shukla, Vinay, Bui, Liem T, Novi, Giacomo, van Dongen, Joost T, Licausi, Francesco, Sub Plant-Environment Signaling, Plant-Environment Signaling, Weits, Daan A, Zhou, Lina, Giuntoli, Beatrice, Carbonare, Laura Dalle, Iacopino, Sergio, Piccinini, Luca, Lombardi, Lara, Shukla, Vinay, Bui, Liem T, Novi, Giacomo, van Dongen, Joost T, and Licausi, Francesco

Published
2023

25. Acquisition of hypoxia inducibility by oxygen sensing N‐terminal cysteine oxidase in spermatophytes

Author

Weits, Daan A., primary, Zhou, Lina, additional, Giuntoli, Beatrice, additional, Carbonare, Laura Dalle, additional, Iacopino, Sergio, additional, Piccinini, Luca, additional, Lombardi, Lara, additional, Shukla, Vinay, additional, Bui, Liem T., additional, Novi, Giacomo, additional, van Dongen, Joost T., additional, and Licausi, Francesco, additional

Published
2022
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27. Making sense of low oxygen sensing

Author

Bailey-Serres, Julia, Fukao, Takeshi, Gibbs, Daniel J., Holdsworth, Michael J., Lee, Seung Cho, Licausi, Francesco, Perata, Pierdomenico, Voesenek, Laurentius A.C.J., and van Dongen, Joost T.

Published
2012
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35. Acquisition of hypoxia inducibility by oxygen sensing N‐terminal cysteine oxidase in spermatophytes.

Author

Weits, Daan A., Zhou, Lina, Giuntoli, Beatrice, Carbonare, Laura Dalle, Iacopino, Sergio, Piccinini, Luca, Lombardi, Lara, Shukla, Vinay, Bui, Liem T., Novi, Giacomo, van Dongen, Joost T., and Licausi, Francesco

Subjects
CYSTEINE, PHANEROGAMS, GENETIC transcription regulation, HYPOXEMIA, MESSENGER RNA, ARABIDOPSIS thaliana
Abstract

N‐terminal cysteine oxidases (NCOs) use molecular oxygen to oxidise the amino‐terminal cysteine of specific proteins, thereby initiating the proteolytic N‐degron pathway. To expand the characterisation of the plant family of NCOs (plant cysteine oxidases [PCOs]), we performed a phylogenetic analysis across different taxa in terms of sequence similarity and transcriptional regulation. Based on this survey, we propose a distinction of PCOs into two main groups. A‐type PCOs are conserved across all plant species and are generally unaffected at the messenger RNA level by oxygen availability. Instead, B‐type PCOs appeared in spermatophytes to acquire transcriptional regulation in response to hypoxia. The inactivation of two A‐type PCOs in Arabidopsis thaliana, PCO4 and PCO5, is sufficient to activate the anaerobic response in young seedlings, whereas the additional removal of B‐type PCOs leads to a stronger induction of anaerobic genes and impairs plant growth and development. Our results show that both PCO types are required to regulate the anaerobic response in angiosperms. Therefore, while it is possible to distinguish two clades within the PCO family, we conclude that they all contribute to restrain the anaerobic transcriptional programme in normoxic conditions and together generate a molecular switch to toggle the hypoxic response. Summary statement: Hypoxic induction of plant cysteine oxidases has been acquired and fixed in seed plants from ancestral proteins able to initiate the proteolysis of Cys‐initiating protein substrates by the Arg/N‐degron pathway. [ABSTRACT FROM AUTHOR]

Published
2023
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36. Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization

Author

Licausi, Francesco, Kosmacz, Monika, Weits, Daan A., Giuntoli, Beatrice, Giorgi, Federico M., Voesenek, Laurentius A.C.J., Perata, Pierdomenico, and van Dongen, Joost T.

Subjects
Models, Genetic aspects, Causes of, Ubiquitin-proteasome system -- Models, Hypoxia (Aquatic ecology) -- Models -- Causes of -- Genetic aspects, Plant respiration -- Genetic aspects, Plants -- Respiration
Abstract

Tolerance to submergence and low oxygen availability (hypoxia) have been considered to be influenced by different members of subgroup VII of the ERF transcription factor family in Arabidopsis (RAP2.12 (ref. [...], The majority of eukaryotic organisms rely on molecular oxygen for respiratory energy production (1). When the supply of oxygen is compromised, a variety of acclimation responses are activated to reduce the detrimental effects of energy depletion (2-4). Various oxygen-sensing mechanisms have been described that are thought to trigger these responses (5-9), but they each seem to be kingdom specific and no sensing mechanism has been identified in plants until now. Here we show that one branch of the ubiquitin-dependent N-end rule pathway for protein degradation, which is active in both mammals and plants (10,11), functions as an oxygen-sensing mechanism in Arabidopsis thaliana. We identified a conserved amino-terminal amino acid sequence of the ethylene response factor (ERF)-transcription factor RAP2.12 to be dedicated to an oxygen-dependent sequence of post-translational modifications, which ultimately lead to degradation of RAP2.12 under aerobic conditions. When the oxygen concentration is low--as during flooding--RAP2.12 is released from the plasma membrane and accumulates in the nucleus to activate gene expression for hypoxia acclimation. Our discovery of an oxygen-sensing mechanism opens up new possibilities for improving flooding tolerance in crops.

Published
2011
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38. Functional balancing of the hypoxia regulators RAP2.12 and HRA1 takes place in vivo in Arabidopsis thaliana plants

Author

Giuntoli, Beatrice, Licausi, Francesco, van Veen, Hans, Perata, Pierdomenico, Sub Plant Ecophysiology, Plant Ecophysiology, Van Veen lab, Sub Plant Ecophysiology, and Plant Ecophysiology

Subjects
0106 biological sciences, 0301 basic medicine, Transgene, Population, Plant Science, lcsh:Plant culture, Protein oxidation, 01 natural sciences, 03 medical and health sciences, Transcription (biology), ERF-VII transcription factors, Low oxygen, Regulation of anaerobic gene expression, Transcription factor balancing, Trihelix transcription factor family, Arabidopsis, Oxygen homeostasis, Botany, lcsh:SB1-1110, education, Transcription factor, Original Research, education.field_of_study, biology, fungi, food and beverages, Promoter, biology.organism_classification, Cell biology, 030104 developmental biology, 010606 plant biology & botany
Abstract

Plants are known to respond to variations in cellular oxygen availability and distribution by quickly adapting the transcription rate of a number of genes, generally associated to improved energy usage pathways, oxygen homeostasis and protection from harmful products of anaerobic metabolism. In terrestrial plants, such coordinated gene expression program is promoted by a conserved subfamily of ethylene responsive transcription factors called ERF-VII, which act as master activators of hypoxic gene transcription. Their abundance is directly regulated by oxygen through a mechanism of targeted proteolysis present under aerobic conditions, which is triggered by ERF-VII protein oxidation. Beside this, in Arabidopsis thaliana, the activity of the ERF-VII factor RAP2.12 has been shown to be restrained and made transient by the hypoxia-inducible transcription factor HRA1. This feedback mechanism has been proposed to modulate ERF-VII activity in the plant under fluctuating hypoxia, thereby enhancing the flexibility of the response. So far, functional balancing between RAP2.12 and HRA1 has been assessed in isolated leaf protoplasts, resulting in an inverse relationship between HRA1 amount and activation of RAP2.12 target promoters. In the present work, we showed that HRA1 is effective in balancing RAP2.12 activity in whole arabidopsis plants. Examination of a segregating population, generated from RAP2.12 and HRA1 over-expressing plants, led to the first quantitative proof that, over a range of either transgene expression levels, HRA1 counteracts the phenotypic and transcriptional effects of RAP2.12. This report supports the occurrence of fine-tuned regulation of the hypoxic response under physiological growth conditions.

Published
2020

49. Fisiologia vegetale applicata

Author

Lionetti, Vincenzo, Bellincampi, Daniela, Angelini, Riccardo, Balestrini, Raffaella, Barera, Simone, Benedetti, Manuel, Boccaccini, Alessandra, Carpaneto, Armando, Cona, Alessandra, Conti, Stefano, Dall'Osto, Luca, De Caroli, Monica, Fiorilli, Valentina, Fraudenziali, Ilaria, Giuntoli, Beatrice, Licausi, Francesco, Malacarne, Giulia, Martignago, Damiano, Morosinotto, Tomas, Moser, Claudio, Pilati, Stefania, Piro, Gabriella, Ricci, Ada, Rigano, Maria Manuela, Rolli, Enrico, Tavladoraki, Paraskevi, Torelli, Anna, and Trifilò, Patrizia

Published
2020

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