89 results on '"Liaqat Hussain"'
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2. Diterpenoid Tanshinones Can Inhibit Lung Cancer Progression by Improving the Tumor Microenvironment and Downregulation of NF-κB Expression
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Hao Xu, Ning Ning Guo, Chen Ying Zhu, Lin Yan Ye, Xing Yi Yan, Yong Qin Liu, Ze Yan Zhang, Guangji Zhang, and Liaqat Hussain
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Chemistry ,QD1-999 - Published
- 2024
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3. Curcumin-loaded soluplus® based ternary solid dispersions with enhanced solubility, dissolution and antibacterial, antioxidant, anti-inflammatory activities
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Memoona Ishtiaq, Hina Manzoor, Ikram Ullah Khan, Sajid Asghar, Muhammad Irfan, Norah A. Albekairi, Abdulrahman Alshammari, Abdulrahman F. Alqahtani, Saad Alotaibi, Rabia Munir, Pervaiz A. Shah, Liaqat Hussain, Muhammad Abubakar Saleem, Fizza Abdul Razzaq, and Syed Haroon Khalid
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Curcumin ,Poloxamer ,Anti-inflammatory agents ,Solubility ,Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer ,Solid dispersions ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Amorphous solid dispersion (ASD) has emerged to be an outstanding strategy among multiple options available for improving solubility and consequently biological activity. Interestingly several binary SD systems continue to exhibit insufficient solubility over time. Therefore, the goal of current research was to design ternary amorphous solid dispersions (ASDs) of hydrophobic model drug curcumin (CUR) to enhance the solubility and dissolution rate in turn, presenting enhanced anti-bacterial, antioxidant and anti-inflammatory activity. For this purpose several ternary solid dispersions (TSDs) consisting of Soluplus®, Syloid® XDP 3150, Syloid® 244 and Poloxamer® 188 in combination with HPMC E5 (binary carrier) were prepared using solvent evaporation method. Both solubility and dissolution testing of prepared solid dispersion were performed to determine the increase in solubility and dissolution. Solid state investigation was carried out utilizing infrared spectroscopy, also known as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM),Differential scanning calorimetry (DSC) and X-ray diffraction (XRD).Optimized formulations were also tested for their biological effectiveness including anti-bacterial, anti-oxidant and anti-inflammatory activity. Amid all Ternary formulations F3 entailing 20 % soluplus® remarkably improved the solubility (186 μg/ml ± 3.95) and consequently dissolution (91 % ± 3.89 %) of curcumin by 3100 and 9 fold respectively. These finding were also supported by FTIR, SEM, XRD and DSC. In-vitro antibacterial investigation of F3 also demonstrated significant improvement in antibacterial activity against both gram positive (Staphylococcus aureus, Bacillus cereus) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. Among all the tested strains Staphylococcus aureus was found to be most susceptible with a zone of inhibition of 24 mm ± 2.87. Antioxidant activity of F3 was also notably enhanced (93 % ± 5.30) in contrast to CUR (69 % ± 4.79). In vitro anti-inflammatory assessment also exhibited that F3 markedly protected BSA (bovine serum albumin) from denaturation with percent BSA inhibition of 80 % ± 3.16 in comparison to CUR (49 % ± 2.91). Hence, F3 could be an effective solid dispersion system for the delivery of model hydrophobic drug curcumin.
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- 2024
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4. Phytochemicals-based investigation of Rubia cordifolia pharmacological potential against letrozole-induced polycystic ovarian syndrome in female adult rats: In vitro, in vivo and mechanistic approach
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Jiangsheng Zhang, Khadeja Arshad, Rida Siddique, Hao Xu, Abdulrahman Alshammari, Norah A. Albekairi, Rizwan Rashid Bazmi, Liaqat Hussain, and Guiyuan Lv
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PCOS ,Rubia cordifolia ,Letrozole ,Hormonal profile ,Weight reduction ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Polycystic Ovarian Syndrome (PCOS) is a metabolic, reproductive, and endocrine disorder affecting women of fertile age. This study aimed to formulate a phytochemicals-based standardized aqueous ethanolic extract of Rubia cordifolia (SERC) to explore its pharmacological potential in PCOS-induced female rats and elucidate its mechanism. HPLC analysis revealed the presence of phytochemicals such as chlorogenic acid, p-coumaric acid, gallic acid, and kaempferol. Thirty female adult rats were divided into two groups for induction of PCOS (5 female rats in the normal control group + 25 female rats in the disease-induced group). PCOS was induced by administering letrozole (1 mg/kg p.o.) for 6 weeks. After PCOS induction, animals of the disease-induced group were divided into five groups: one group used as disease control (PCOS) group, one group on metformin (20 mg/kg), and three groups on SERC (200, 400, and 600 mg/kg). Histopathological analysis showed that PCOS induction reduced corpus luteum and developing follicles and increased cystic follicles. In comparison, SERC treatment improved ovulation with more primary and developing follicles. SERC reduced the serum insulin, LH surge, and testosterone levels while improving the FSH, estrogen, and progesterone serum levels. SERC significantly improved the oxidation status of the liver and normalized the lipid profile and liver function markers. In conclusion, SERC treated PCOS, and the suggested mechanism might be the restoration of aromatase activity and background inflammatory status improvement in ovaries.
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- 2024
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5. Phytochemical-Based Study of Ethanolic Extract of Saraca asoca in Letrozole-Induced Polycystic Ovarian Syndrome in Female Adult Rats
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Na Bu, Alina Jamil, Liaqat Hussain, Abdulrahman Alshammari, Thamer H. Albekairi, Metab Alharbi, Ayesha Jamshed, Rizwan Rashid Bazmi, and Anam Younas
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Chemistry ,QD1-999 - Published
- 2023
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6. Pharmacological Potential of Hippophae rhamnoides L. Nano-Emulsion for Management of Polycystic Ovarian Syndrome in Animals’ Model: In Vitro and In Vivo Studies
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Liaqat Hussain, Saba Rana, Ghulam Abbas, Abdulrahman Alshammari, Abdullah F. Alasmari, Metab Alharbi, Muhammad Toseef Zahid, and Muhammad Irfan
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Chemistry ,QD1-999 - Published
- 2023
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7. Pharmacological Assessment of Aqueous Ethanolic Extract of Against Haloperidol-Induced Parkinson’s Like Symptoms in Animal Model: A Dose-Dependent Study With Mechanistic Approach
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Jiangyu Han, Xu Hao, Mishal Fatima, Zunera Chauhdary, Ayesha Jamshed, Hafiz Muhammad Abdur Rahman, Rida Siddique, Muhammad Asif, Saba Rana, and Liaqat Hussain
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Introduction: Parkinson’s disease (PD) is characterized by dopamine deficiency in the corpus striatum due to the degeneration of dopaminergic neurons in the substantia nigra. Symptoms include bradykinesia, resting tremors, unstable posture, muscular rigidity, and a shuffled gait. Thalictrum foetidum is traditionally used for neurodegenerative disorders. Objectives: This study aimed to explore the therapeutic potential of aqueous ethanolic extract of Thalictrum foetidum (AETF) against Parkinson-like symptoms and to investigate its underlying mechanism. Methodology: Thirty-six albino mice were randomly divided into 6 groups (n = 6): normal control, disease control, standard treatment (levodopa/carbidopa, 100/25 mg/kg), and 3 treatment groups (AETF at 200, 400, and 600 mg/kg). One hour before treatment, haloperidol (1 mg/kg, i. p .) was administered to induce Parkinson’s disease in all groups except the normal control group. Results: Behavioral analysis showed significant improvement ( P < .001) in motor function, muscular coordination, and reduced muscular rigidity and tremors. AETF also reduced oxidative stress. Histological examination of the brain showed reduced Lewy bodies, neurofibrillary tangles, and plaque formation. Conclusion: AETF alleviated PD symptoms by reducing neurodegeneration, modulating oxidative stress, and inhibiting the expression of nuclear factor-κB (NF-κB) and associated inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6).
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- 2024
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8. Cichorium intybus L. significantly alleviates cigarette smoke-induced acute lung injury by lowering NF-κB pathway activation and inflammatory mediators
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Nadia Hussain, Nadia Ikram, Kashif ur Rehman Khan, Liaqat Hussain, Ali M. Alqahtani, Taha Alqahtani, Musaddique Hussain, Muath Suliman, Mohammad Y. Alshahrani, and Basel Sitohy
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ALI ,TOS ,MPO ,NF-κB p65 ,IL-6 ,IL-1β ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Cigarette smoke (CS) is one of the primary causes of acute lung injury (ALI) via provoking pulmonary inflammation and oxidative stress. Despite substantial studies, no effective treatment for ALI is presently available. Purpose: New prospective treatment options for ALI are required. Thus, this project was designed to investigate the in vivo and in vitro protective effects of 70 % methanolic-aqueous crude extract of whole plant of Cichorium intybus (Ci.Mce) against CS-induced ALI. Study design: /methods: Initially, male Swiss albino mice were subjected to whole-body CS exposure for 10 continuous days to prepare CS-induced ALI models. Normal saline (10 mL/kg), Ci.Mce (100, 200, 300 mg/kg), and Dexamethasone (1 mg/kg) were orally administered to respective animal groups 1 h prior to CS-exposure. 24 hrs after the last CS-exposure, BALF and lungs were harvested to study the key characteristics of ALI. Next, HPLC analysis was done to explore the phytoconstituents. Results: Ci.Mce exhibited significant reductions in lung macrophage and neutrophil infiltration, lung weight coefficient, and albumin exudation. Additionally, it effectively ameliorated lung histopathological alterations and hypoxemia. Notably, Ci.Mce exerted inhibitory effects on the excessive generation of IL-6, IL-1β, and KC in both CS-induced ALI murine models and CSE-stimulated RAW 264.7 macrophages. Noteworthy benefits included the attenuation of oxidative stress induced by CS, evidenced by decreased levels of MDA, TOS, and MPO, alongside enhanced TAC production. Furthermore, Ci.Mce demonstrated a marked reduction in CS-induced NF-κB expression, both in vivo and in vitro. Conclusion: Consequently, Cichorium intybus could be a therapeutic option for CS-induced ALI due to its ability to suppress inflammatory reactions, mitigate oxidative stress, and quell NF-κB p65 activation.
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- 2023
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9. Correction to 'Diterpenoid Tanshinones Can Inhibit Lung Cancer Progression by Improving the Tumor Microenvironment and Downregulation of NF-κB Expression'
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Hao Xu, Ning Ning Guo, Chen Ying Zhu, Lin Yan Ye, Xing Yi Yan, Yong Qin Liu, Ze Yan Zhang, Liaqat Hussain, and Guangji Zhang
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Chemistry ,QD1-999 - Published
- 2024
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10. Nephroprotective Potential of a Standardized Extract of Bambusa arundinacea: In Vitro and In Vivo Studies
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Saba Muzher Iqbal, Liaqat Hussain, Musaddique Hussain, Haseeb Akram, Muhammad Asif, Ayesha Jamshed, Ammara Saleem, and Rida Siddique
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Chemistry ,QD1-999 - Published
- 2022
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11. Dose Dependent Effects of Aqueous Extract of Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration
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Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H. Lodhi, Muhammad Q. Barkat, Muhammad Riaz, and Alina Jamil
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Background Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women. Purpose This study aimed to explore the potential of aqueous extract of Garcinia cambogia Desr. (AEGC) in PCOS. Methodology The HPLC was used to determine the phytoconstituents present in Garcinia cambogia. Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis. Results HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement ( P < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly ( P < .05) increased. Lipid profile and LFTs were also improved. Conclusions The study validated the potential of Garcinia cambogia in the management of PCOS.
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- 2023
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12. Topical Delivery of Terbinafine HCL Using Nanogels: A New Approach to Superficial Fungal Infection Treatment
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Shams ul Hassan, Ikrima Khalid, Liaqat Hussain, Mohammad T. Imam, and Imran Shahid
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dermatophytosis ,nanogels ,terbinafine HCL ,acrylic acid ,gelatin ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
This study investigated pH-responsive Terbinafine HCL (TBH)-loaded nanogels as a new approach to treating superficial fungal infections. Acrylic acid (AA) is a synthetic monomer that was crosslinked with a natural polymer (gelatin) using a free radical polymerization technique to fabricate gelatin-g-poly-(acrylic acid) nanogels. Ammonium persulphate (APS) and N, N′-methylene bisacrylamide (MBA) were used as the initiator and crosslinker, respectively. Developed gelatin-g-poly-(acrylic acid) nanogels were evaluated for the swelling study (pH 1.2, 5, 7.4), DEE, particle size, FTIR, thermal stability (TGA, DSC), XRD, SEM, DEE, and in vitro drug release study to obtain optimized nanogels. Optimized nanogels were incorporated into 1% HPMC gel and then evaluated in comparison with Lamisil cream 1% for TBH stratum corneum retention, skin irritation, and in vitro and in vivo antifungal activity studies. Optimized nanogels (AAG 7) demonstrated a 255 nm particle size, 82.37% DEE, pH-dependent swelling, 92.15% of drug release (pH) 7.4 within 12 h, and a larger zone of inhibition compared to Lamisil cream. HPMC-loaded nanogels significantly improved the TBH skin retention percentage, as revealed by an ex vivo skin retention study, indicating the usefulness of nanogels for topical use. In vivo studies conducted on animal models infected with a fungal infection have further confirmed the effectiveness of nanogels compared with the Lamisil cream. Hence, Gelatin-g-poly-(acrylic acid) nanogels carrying poorly soluble TBH can be a promising approach for treating superficial fungal infections.
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- 2023
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13. Dose Dependent Effects of Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: , and Mechanistic Studies
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Malik Saadullah, Sania Arif, Liaqat Hussain, Muhammad Asif, and Umair Khurshid
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Therapeutics. Pharmacology ,RM1-950 - Abstract
The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration ( P < .001) while MDA showed significant decrease ( P < .05). The AChE level was significantly reduced ( P < .05) at 500 mg/kg while neurotransmitters were significantly improved ( P < .001) in a dose-dependent fashion. The ELISA results showed significant ( P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD.
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- 2022
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14. Polycystic Ovary Syndrome: A Disorder of Reproductive Age, Its Pathogenesis, and a Discussion on the Emerging Role of Herbal Remedies
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Ling-Hui Zeng, Saba Rana, Liaqat Hussain, Muhammad Asif, Malik Hassan Mehmood, Imran Imran, Anam Younas, Amina Mahdy, Fakhria A. Al-Joufi, and Shaymaa Najm Abed
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PCOS ,endocrine abnormality ,metabolic disorder ,infertility ,reproduction ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Polycystic ovary syndrome (PCOS) is a very common, complex, and heterogeneous endocrine disorder of women that involves a combination of environmental and genetic factors. PCOS affects women of growing age particularly at the early to late reproductive stage (15–35 years). Currently, PCOS affects 1 in every 10 women worldwide. It is characterized majorly by a raised level of androgens such as testosterone and a large number of ovarian cysts (more than 10) that cause anovulation, infertility, and irregular menstrual cycle. PCOS is also related to other endocrine and metabolic abnormalities, such as obesity, hirsutism, acne, diabetes, insulin resistance, and glucose impairment. PCOS can be treated with allopathic, ayurvedic, and natural or herbal medications along with lifestyle modifications. Herbal medicines remained in demand for numerous reasons such as high cost and side effects associated with the use of allopathic medicine and our traditional norms, which have helped humans to use more herbal products for their health benefits. Estrogenic and nonestrogenic phytochemicals present in various plant species such as Glycyrrhiza glabra L. [Fabaceae], Aloe vera (L.) Burm. f. [Asphodelaceae], Silybum marianum (L.). Gaertn. [Asteraceae], Serenoa repens (W.Bartram) Small [Arecaceae], Actaea racemosa L. [Ranunculaceae], and Angelica sinensis (Oliv.) Diels [Apiaceae] are effective and harmless. Herbal medicines are found to be cost-effective, efficacious, and a highly esteemed source of management/treatment for PCOS than allopathic medicines. In this literature review, diagnosis, signs, and symptoms of PCOS; causes of hormonal imbalance; and risk factors associated with PCOS and their management are discussed briefly, and the focus was to find out the role of herbal remedies in PCOS management.
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- 2022
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15. Verapamil attenuates oxidative stress and inflammatory responses in cigarette smoke (CS)-induced murine models of acute lung injury and CSE-stimulated RAW 264.7 macrophages via inhibiting the NF-κB pathway
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Ximei Wu, Musaddique Hussain, Shahzada Khurram Syed, Malik Saadullah, Ali M. Alqahtani, Taha Alqahtani, Afaf Aldahish, Mobeen Fatima, Saira Shaukat, Liaqat Hussain, Qurratulain Jamil, Imran Mukhtar, Kashif-ur-Rehman Khan, and Ling-Hui Zeng
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Acute lung injury ,ARDS ,Verapamil ,IL-6 ,TNF-α ,NF-κB ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), severe form of ALI, are characterized by overwhelming of lung inflammation, and no treatment is currently available to treat ALI/ARDS. Cigarette smoke (CS) is one of the prime causes to induce ALI/ARDS via oxidative stress. Despite extensive research, no appropriate therapy is currently available to treat ALI/ARDS. Hence, new potential approaches are needed to treat ALI/ARDS. Consequently, this project was designed to explore the protective effects of verapamil against CS-induced ALI by in vivo and in vitro method. In vivo data obtained from respiratory mechanics, pulmonary morphometric analyses and lung histopathology revealed that verapamil dose-dependently and strikingly decreased the lung weight coefficient, attenuated the albumin exudation into lungs, minimized the infiltration of macrophages and neutrophils into lungs, reduced the pro-inflammatory cytokines (tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and keratinocyte chemoattractant (KC)) production, and improved the hypoxemia and lung histopathological changes. Similarly, verapamil also reduced the production of TNF-α, IL-6 and KC from cigarette smoke extract (CSE)-stimulated RAW 264.7 macrophage. Importantly, verapamil dose-dependently and remarkably suppressed the CS-induced oxidative stress via not only reducing the myeloperoxidase (MPO) activity of lungs, total oxidative stress (TOS) and malondialdehyde (MDA) content in the lungs and supernatant of RAW 264.7 macrophage but also improving total antioxidant capacity (TAC) and superoxide dismutase (SOD) production. Finally, verapamil strikingly decreased the NF-κB expression both in in vivo and in vitro models. Hence, verapamil has positive therapeutic effects against CS-induced ALI via suppressing uncontrolled inflammatory response, oxidative stress and NF-κB p65 signaling.
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- 2022
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16. Development and Evaluation of pH-Responsive Pluronic F 127 Co-Poly- (Acrylic Acid) Biodegradable Nanogels for Topical Delivery of Terbinafine HCL
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Shams ul Hassan, Ikrima Khalid, Liaqat Hussain, Kashif Barkat, and Ikram Ullah Khan
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Research aimed to develop and evaluate biodegradable, pH-responsive chemically cross-linked Pluronic F127 co-poly- (acrylic acid) nanogels for dermal delivery of Terbinafine HCL (TBH) to increase its permeability and as a new approach to treat skin fungal infections. TBH-loaded nanogels were successfully synthesized from acrylic acid (AA) and Pluronic F127 by free-radical copolymerization technique using N,N′-methylene bisacrylamide (MBA) as crosslinker and ammonium persulphate (APS) as initiator. Prepared nanogels exhibited 93.51% drug entrapment efficiency (DEE), 45 nm particle size, pH-dependent swelling and release behavior. Nanogels were characterized using different physicochemical techniques. The ex-vivo skin retention studies through rat skin showed about 42.34% drug retention from nanogels while 1% Lamisil cream (marketed product) showed about 26.56% drug retention. Moreover, skin irritation studies showed that nanogels were not irritating. Nanogels showed improved in-vitro antifungal activity against Candida albicans compared to commercial product. In-vivo studies on rats infected with Candida albicans confirmed superiority of nanogels over 1% Lamisil for eradication of fungal infection. This confirms that TBH loaded in Pluronic F127 co-poly-(acrylic acid) nanogels provided greater targetibility and cure rates of poorly soluble TBH in animal model and hence nanogels could be a potential carrier for effective topical delivery of TBH for skin fungal infection treatment.
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- 2022
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17. Hedgehog Signaling: Linking Embryonic Lung Development and Asthmatic Airway Remodeling
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Ling-Hui Zeng, Muhammad Qasim Barkat, Shahzada Khurram Syed, Shahid Shah, Ghulam Abbas, Chengyun Xu, Amina Mahdy, Nadia Hussain, Liaqat Hussain, Abdul Majeed, Kashif-ur-Rehman Khan, Ximei Wu, and Musaddique Hussain
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hedgehog signaling ,lung development ,asthmatic airway remodeling ,targets pathogen ,repairment in tissue and immune system ,Cytology ,QH573-671 - Abstract
The development of the embryonic lung demands complex endodermal–mesodermal interactions, which are regulated by a variety of signaling proteins. Hedgehog (Hh) signaling is vital for lung development. It plays a key regulatory role during several morphogenic mechanisms, such as cell growth, differentiation, migration, and persistence of cells. On the other hand, abnormal expression or loss of regulation of Hh signaling leads to airway asthmatic remodeling, which is characterized by cellular matrix modification in the respiratory system, goblet cell hyperplasia, deposition of collagen, epithelial cell apoptosis, proliferation, and activation of fibroblasts. Hh also targets some of the pathogens and seems to have a significant function in tissue repairment and immune-related disorders. Similarly, aberrant Hh signaling expression is critically associated with the etiology of a variety of other airway lung diseases, mainly, bronchial or tissue fibrosis, lung cancer, and pulmonary arterial hypertension, suggesting that controlled regulation of Hh signaling is crucial to retain healthy lung functioning. Moreover, shreds of evidence imply that the Hh signaling pathway links to lung organogenesis and asthmatic airway remodeling. Here, we compiled all up-to-date investigations linked with the role of Hh signaling in the development of lungs as well as the attribution of Hh signaling in impairment of lung expansion, airway remodeling, and immune response. In addition, we included all current investigational and therapeutic approaches to treat airway asthmatic remodeling and immune system pathway diseases.
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- 2022
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18. Heat Transfer Augmentation through Different Jet Impingement Techniques: A State-of-the-Art Review
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Liaqat Hussain, Muhammad Mahabat Khan, Manzar Masud, Fawad Ahmed, Zabdur Rehman, Łukasz Amanowicz, and Krzysztof Rajski
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heat transfer enhancement ,jet impingement ,convective heat transfer ,active cooling ,fluid surface interaction ,Technology - Abstract
Jet impingement is considered to be an effective technique to enhance the heat transfer rate, and it finds many applications in the scientific and industrial horizons. The objective of this paper is to summarize heat transfer enhancement through different jet impingement methods and provide a platform for identifying the scope for future work. This study reviews various experimental and numerical studies of jet impingement methods for thermal-hydraulic improvement of heat transfer surfaces. The jet impingement methods considered in the present work include shapes of the target surface, the jet/nozzle–target surface distance, extended jet holes, nanofluids, and the use of phase change materials (PCMs). The present work also includes both single-jet and multiple-jet impingement studies for different industrial applications.
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- 2021
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19. Hepatoprotective effects of Sapium sebiferum in paracetamol-induced liver injury
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Liaqat Hussain, Muhammad Sajid Hamid Akash, Madeha Tahir, and Kanwal Rehman
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Hepatotoxicity ,Paracetamol ,Sapium sebiferum ,Silymarin ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Sapium sebiferum leaves were used to determine its hepatoprotective effects against paracetamol-induced hepatotoxicity in mice. A dose dependent study was conducted using two different doses (200 mg/kg and 400 mg/kg) of the extract of S. sebiferum against toxic effects of paracetamol (500 mg/kg) in experimental animal model. Silymarin (50 mg/kg) was used as standard drug to compare therapeutic effects of S. sebiferum with control and paracetamol-treated groups. Paracetamol significantly increased the serum levels of liver enzyme markers like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, and direct bilirubin. The extract showed protective effects by normalizing the liver enzymes markers in a dose dependent manner. Histopathological results confirmed the hepatoprotective effects of leaves of S. sebiferum. We conclude that leaves of S. sebiferum have strong hepatoprotective effects against paracetamol-induced liver injury and can be used in liver injuries caused by drug-induced toxicity.
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- 2015
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20. Phenylarsine Oxide Can Induce the Arsenite-Resistance Mutant PML Protein Solubility Changes
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Yu Han Jiang, Ye Jia Chen, Chao Wang, Yong Fei Lan, Chang Yang, Qian Qian Wang, Liaqat Hussain, Yasen Maimaitiying, Islam Khairul, and Hua Naranmandura
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arsenite ,acute promyelocytic leukemia ,arsenic trioxide ,monomethylarsonous acid ,dimethylarsinous acid ,phenylarsine oxide ,trivalent arsenicals ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Arsenic trioxide (As2O3) has recently become one of the most effective drugs for treatment of patient with acute promyelocytic leukemia (APL), and its molecular mechanism has also been largely investigated. However, it has been reported that As2O3 resistant patients are frequently found in relapsed APL after consolidation therapy, which is due to the point mutations in B-box type 2 motifs of promyelocytic leukemia (PML) gene. In the present study, we for the first time establish whether organic arsenic species phenylarsine oxide (PAO) could induce the mutant PML-IV (A216V) protein solubility changes and degradation. Here, three different PML protein variants (i.e., PML-IV, PML-V and mutant PML-A216V) were overexpressed in HEK293T cells and then exposed to PAO in time- and dose-dependent manners. Interestingly, PAO is found to have potential effect on induction of mutant PML-IV (A216V) protein solubility changes and degradation, but no appreciable effects were found following exposure to high concentrations of iAsIII, dimethylarsinous acid (DMAIII) and adriamycin (doxorubicin), even though they cause cell death. Our current data strongly indicate that PAO has good effects on the mutant PML protein solubility changes, and it may be helpful for improving the therapeutic strategies for arsenic-resistant APL treatments in the near future.
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- 2017
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21. Antihyperglycemic and hypolipidemic potential of Caesalpinia decapetala in alloxan-induced diabetic rabbits
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Liaqat Hussain, Muhammad Imran Qadir, and Shafiq-ur- Rehman
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Antihyperglycemic ,Caesalpinia decapetala ,Diabetes mellitus ,Lipidemia ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The anti-hyperglycemic, anti-hyperlipidemic, kidney and hepatoprotective potential of Caesalpinia decapetala were evaluated in alloxan-induced diabetic rabbits. The plant extract showed concentration dependant significant (p
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- 2014
22. Hepatoprotective effects of methanolic extract of Alcea rosea against acetaminophen-induced hepatotoxicity in mice
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Liaqat Hussain, Muhammad Sajid Hamid Akash, Madeha Tahir, Kanwal Rehman, and Khwaja Zafar Ahmed
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Acetaminophen ,Alcea rosea ,Bilirubin ,Hepatotoxicity ,Liver enzyme marker ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The aim of this study was to evaluate the hepatoprotective effects of Alcea rosea against acetaminophen-induced hepatotoxicity in mice. Aqueous methanolic extract of A. rosea were given orally for 7 consecutive days followed by daily toxic dose of acetaminophen. At the end of treatment period, evaluation of hepatoprotective activity of A. rosea was done on basis of levels of liver enzyme markers (aminotransferases, alkaline phosphatase and bilirubin) and histopathological examination of liver tissues. Acetaminophen significantly increased serum levels of liver enzyme markers whereas, the extract of A. rosea significantly reduced serum levels of elevated liver enzyme markers in dose-dependent manner compared to acetaminophen treated mice group. Histopathological examination of liver tissues also supported the protective effects of A. rosea on liver enzyme markers. We conclude that extract of A. rosea has strong hepatoprotective effects against acetaminophen-induced hepatotoxicity; thereby, affirming its traditional therapeutic role in liver injury.
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- 2014
23. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity
- Author
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Mohsin Ali, M. Imran Qadir, Mohammad Saleem, Khalid Hussain Janbaz, Humaira Gul, Liaqat Hussain, and Bashir Ahmad
- Subjects
Hepatoprotective ,Serum enzymes ,Hepatotoxicity ,Paracetamol ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Convolvulus arvensis is traditionally used as laxative. Its decoction is used in cough, flu, jaundice and in skin diseases. It is also used to treat the painful joints, inflammation and swelling. The current study was conducted to determine its hepatoprotective activity. The results showed that extract of C. arvensis (200 mg/kg and 500 mg/kg) produced significant (p
- Published
- 2013
24. Unsteady and Incompressible Magneto-Hydrodynamics Blood Flow in an Inclined Cylindrical Channel
- Author
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Liaqat, Hussain, primary, Salah, Uddin, additional, and Asif, Shahzad, additional
- Published
- 2023
- Full Text
- View/download PDF
25. Wound Healing Potential and In Silico Appraisal of Convolvulus arvensis L. Methanolic Extract
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Uzma Saleem, Sana Khalid, Shingraf Zaib, Fareeha Anwar, Muhammad Furqan Akhtar, Liaqat Hussain, Ammara Saleem, and Bashir Ahmad
- Subjects
Article Subject ,General Immunology and Microbiology ,General Medicine ,General Biochemistry, Genetics and Molecular Biology - Abstract
Convolvulus arvensis L. is rich in phenolic compounds and traditionally used to treat wounds, skin ulcer, and inflammation. The current study is aimed at scientifically potentiating its traditional wound healing use. The methanolic extract of C. arvensis stem (CaME) was analyzed for HPLC and GC-MS analyses. The binding modes of active compounds were investigated against protein targets glycogen synthase kinase-3β (GSK-3β), transforming growth factor-beta (TGF-β), c-myc, and β-catenin by molecular docking followed by molecular dynamic simulations which revealed some conserved mode of binding as reported in crystal structures. The antioxidant potential of CaME was evaluated by in vitro methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, and ferric reducing power assays. Ointment formulations of 10 and 20% CaME were applied topically and evaluated for wound healing potency against the excisional wound on the skin of Wistar rats. Gentamycin (0.1%) served as standard therapy. The healing process was observed for 20 days in the form of wound size and epithelialization followed by histopathological evaluation of the wound area. Chemical characterization showed the presence of 7-hexadecenoic acid, 2-hexadecylicosan-1-ol, quercetin, gallic acid, ferulic acid, and other compounds. The plant extract exhibited significant in vitro antioxidant activity. The animals treated with 10% ointment showed moderate healing, whereas the treatment with 20% CaME revealed healing potential comparable to the standard 0.1% gentamycin as coevidenced from histopathological evaluation of skin. The study corroborates promising potential of C. arvensis on the healing of wounds, which possibly will be attributed to its antioxidant activity, fatty acids, quercetin, and gallic and caffeic acids, and binding potential of its phytoconstituents (phenolic acids) with wound healing targets.
- Published
- 2022
26. Hyperthermia promotes degradation of the acute promyelocytic leukemia driver oncoprotein ZBTB16/RARα
- Author
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Qian-qian Wang, Liaqat Hussain, Pei-han Yu, Chang Yang, Chen-ying Zhu, Ya-fang Ma, Si-chun Wang, Tao Yang, Yuan-yuan Kang, Wen-juan Yu, Yasen Maimaitiyiming, and Hua Naranmandura
- Subjects
Pharmacology ,Pharmacology (medical) ,General Medicine - Abstract
The acute promyelocytic leukemia (APL) driver ZBTB16/RARα is generated by the t(11;17) (q23;q21) chromosomal translocation, which is resistant to combined treatment of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) or conventional chemotherapy, resulting in extremely low survival rates. In the current study, we investigated the effects of hyperthermia on the oncogenic fusion ZBTB16/RARα protein to explore a potential therapeutic approach for this variant APL. We showed that Z/R fusion protein expressed in HeLa cells was resistant to ATO, ATRA, and conventional chemotherapeutic agents. However, mild hyperthermia (42 °C) rapidly destabilized the ZBTB16/RARα fusion protein expressed in HeLa, 293T, and OCI-AML3 cells, followed by robust ubiquitination and proteasomal degradation. In contrast, hyperthermia did not affect the normal (i.e., unfused) ZBTB16 and RARα proteins, suggesting a specific thermal sensitivity of the ZBTB16/RARα fusion protein. Importantly, we found that the destabilization of ZBTB16/RARα was the initial step for oncogenic fusion protein degradation by hyperthermia, which could be blocked by deletion of nuclear receptor corepressor (NCoR) binding sites or knockdown of NCoRs. Furthermore, SIAH2 was identified as the E3 ligase participating in hyperthermia-induced ubiquitination of ZBTB16/RARα. In short, these results demonstrate that hyperthermia could effectively destabilize and subsequently degrade the ZBTB16/RARα fusion protein in an NCoR-dependent manner, suggesting a thermal-based therapeutic strategy that may improve the outcome in refractory ZBTB16/RARα-driven APL patients in the clinic.
- Published
- 2022
27. Therapeutic Investigation of Standardized Aqueous Methanolic Extract of Bitter Melon (Momordica charantia L.) for Its Potential against Polycystic Ovarian Syndrome in Experimental Animals’ Model: In Vitro and In Vivo Studies
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Liaqat Hussain, Noor Aamir, Musaddique Hussain, Muhammad Asif, Zunera Chauhdary, Faiza Manzoor, Rida Siddique, and Muhammad Riaz
- Subjects
Article Subject ,Complementary and alternative medicine - Abstract
Polycystic ovarian syndrome (PCOS) is an heterogenous, endocrine, metabolic, and multidisciplinary disorder of reproductive-aged females that aggravates insulin resistance, hyperandrogenism, obesity, menstrual irregularities, and infertility. Bitter melon is consumed as vegetable in various parts of the world. The purpose of this study was to provide the rationale for the folkloric uses of bitter melon (Momordica charantia L.) in reproductive abnormalities. HPLC analysis of standardized aqueous methanolic extract of bitter melon revealed the presence of various phytochemicals such as quercetin, gallic acid, benzoic acid, chlorogenic acid, syringic acid, p-coumaric acid, ferulic acid, and cinnamic acid. Twenty-five Swiss albino adult female rats (120–130 g) were acquired and divided into two groups (5 + 20). Letrozole (1 mg/kg p.o.) was used for four weeks to induce PCOS in twenty rats. Disease induction was confirmed by vaginal smear cytology analysis under the microscope. Animals were further divided into four groups, with one group as PCOS group, and the remaining three are treated with standardized extract of bitter melon (500 mg/kg p.o.), bitter melon plus metformin (500 mg/kg p.o.), and metformin alone for the period of next four weeks. After four weeks, the rats were euthanized at diestrus stage. Ovaries of the experimental animals were removed and fixed in 10% buffered formalin, and blood samples were obtained from direct cardiac puncture and stored. Ovaries histopathological analysis showed cystic follicles (9–10) in PCOS group, while, in all the treatment groups, we found developing and mature follicles. Similarly, hormone analysis showed significant ( p < 0.001 ) reduction of LH surge, insulin, and testosterone levels and improvement in FSH levels. Lipid profile and antioxidant enzymes status were also significantly ( p < 0.001 ) improved. In conclusion, the study validates the bitter melon potential as an insulin sensitizer and ovulation enhancer and authenticates its potential in PCOS management.
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- 2022
28. Pharmacological Potential of the Standardized Methanolic Extract of Prunus armeniaca L. in the Haloperidol-Induced Parkinsonism Rat Model
- Author
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Uzma Saleem, Liaqat Hussain, Faiza shahid, Fareeha Anwar, Zunera Chauhdary, and Aimen Zafar
- Subjects
Article Subject ,Complementary and alternative medicine - Abstract
Parkinson’s disease (PD) is a complex, age-related neurodegenerative disease that causes neuronal loss and dysfunction over time. An imbalance of redox potential of oxidative stress in the cell causes neurodegenerative diseases and dysfunction of neurons. Plants are a rich source of bioactive substances that attenuate oxidative stress in a variety of neurological disorders. The aim of the present study was to evaluate the Prunus armeniaca L. methanolic extract (PAME) for anti-Parkinson activity in rats. PD was induced with haloperidol (1 mg/kg, IP). The PAME was administered orally at 100, 300, and 800 mg/kg dose levels for 21 days. Behavioral studies (catalepsy test, hang test, open-field test, narrow beam walk, and hole-board test), oxidative stress biomarkers (SOD, CAT, GSH, and MDA) levels, neurotransmitters (dopamine, serotonin, and noradrenaline) levels, and acetylcholinesterase activity were quantified in the brain homogenate. Liver function tests (LFTs), renal function tests (RFTs), complete blood count (CBC), and lipid profiles were measured in the blood/serum samples to note the side effects of PAME at the selected doses. Histopathological analysis was performed on the brain (anti-PD study), liver, heart, and kidney (to check the toxicity of PAME on these vital organs). Motor functions were improved in the behavioral studies. Dopamine, serotonin, and noradrenaline levels were significantly increased ( P < 0.001 ), whereas the level of acetylcholinesterase was decreased significantly ( P < 0.001 ). The levels of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) were increased, while malondialdehyde (MDA) and nitrite levels were decreased in the PAME-treated groups significantly compared with the disease control group, hence reducing oxidative stress. The incidence of toxicity was determined by biochemical analysis of LFT and RFT biomarkers testing. The histopathological analysis indicated that neurofibrillary tangles and plaques decreased in a dose-dependent manner in the PAME-treated groups. Based on the data, it is concluded that PAME possessed good anti-Parkinson activity, rationalizing the plant’s traditional use as a neuroprotective agent.
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- 2022
29. Protective Effects of p-CA Against Acute Liver Damage Induced by LPS/D-GalN in Wistar Albino Rats
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Seerat, Mehdi, Fiaz-Ud-Din, Ahmad, Arslan Hussain, Lodhi, Umair, Khurshid, Ahmed Awais, Khalid, Sheikh Safeena, Sidiq, Liaqat, Hussain, and Mirza Shaharyar, Baig
- Subjects
Lipopolysaccharides ,Coumaric Acids ,Anti-Inflammatory Agents ,Pharmaceutical Science ,Galactosamine ,Ascorbic Acid ,Antioxidants ,Malondialdehyde ,Drug Discovery ,Animals ,Aspartate Aminotransferases ,Rats, Wistar ,Pharmacology ,Drug Design, Development and Therapy ,Interleukin-6 ,Superoxide Dismutase ,Tumor Necrosis Factor-alpha ,Alanine Transaminase ,Bilirubin ,Cholesterol, LDL ,Alkaline Phosphatase ,Glutathione ,Rats ,Liver ,Saline Solution ,Chemical and Drug Induced Liver Injury ,Silymarin - Abstract
Seerat Mehdi,1 Fiaz-ud-Din Ahmad,1 Arslan Hussain Lodhi,1 Umair Khurshid,2 Ahmed Awais Khalid,1 Sheikh Safeena Sidiq,1 Liaqat Hussain,3 Mirza Shaharyar Baig1 1Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; 3Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, PakistanCorrespondence: Fiaz-ud-Din Ahmad, Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Khawaja Fareed Campus, Railway Road, Bahawalpur, 63100, Pakistan, Tel +92-320-8402376, Email fiaz.ahmad@iub.edu.pkAim: Liver regulates metabolism of biomolecules and injury of liver causes distortion of metabolic functions. This injury may be oxidative or inflammatory induced by numerous factors including alcohol, pathogens and xenobiotics. This scientific study was planned to investigate the anti-inflammatory and anti-oxidant potential of p-coumaric acid (p-CA) on Lipopolysaccharide/D-Galactosamine (LPS/D-GalN) induced liver injury.Methods: DPPH analysis, reducing power assay and HPLC analysis were performed during in-vitro studies of p-CA. Similarly, in-vivo experiments were performed using Wistar Albino rats. Normal control and intoxicated group received (5mL/kg normal saline p.o), standard treatment groups received ascorbic acid (100mg/kg p.o) and silymarin (25mg/kg p.o), while p-CA treatment groups received (100mg/kg p.o) for 28-days. After completion of 28-days, LPS/D-GalN injection (300 mg D-GalN/kg and 10 μg LPS/kg i.p.) was given at 6th, 12th and 24-hours to all groups except normal control group. Animals were sacrificed; serum and liver samples were harvested and subjected to biochemical and histological examinations, respectively.Results: The results revealed that p-CA possess strong antioxidant activity. Increased levels of leukocyte infiltration (TLC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TBIL), lipid panel (eg TG, TC, LDL-C, VLDL-C), whereas decreased HDL-C levels noticed in LPS/D-GalN groups as compared to normal control groups. Pro-Inflammatory markers (eg TNF-α, IL-6, IL-1β) and lipid peroxidation marker, eg malondialdehyde (MDA) increased while superoxide dismutase (SOD) and reduced glutathione (GSH) levels were decreased significantly in groups treated with LPS/D-GalN. ANOVA with Bonferroni post hoc analysis was used for statistical analysis of. H&E staining was done to assess architectural abnormalities among liver cells.Conclusion: In conclusion, p-CA could ameliorate LPS/D-GalN induced hepatic injury via regulation of immune responses, liver function enzymes, lipid profile, oxidative stress and pro-inflammatory markers.Keywords: p-coumaric acid, lipopolysaccharide, D-galactosamine, oxidative stress, inflammation
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- 2022
30. Role of medicinal herbs and phytochemicals in post burn management
- Author
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Rida Siddique, Malik Hassan Mehmood, Liaqat Hussain, Abdul Malik, Ayesha Sethi, Maryam Farrukh, and Shaneel Kousar
- Subjects
Pharmacology ,Immunology ,Pharmacology (medical) - Published
- 2023
31. Nanofunctional Foods as Immunity Booster in COVID‐19
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Muhammad Qasim Barkat, Ahsan Nawaz, Sana Asghar, Farwa Basit, Musaddique Hussain, Liaqat Hussain, and Ximei Wu
- Published
- 2022
32. Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies
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Asiya Bashir, Muhammad Asif, Malik Saadullah, Mohammad Saleem, Syed Haroon Khalid, Liaqat Hussain, Ikram Ullah Khan, Hafiza Sidra Yaseen, Hafiz Muhammad Zubair, Muhammad Usman Shamas, Raghdaa Al Zarzour, and Tahir Ali Chohan
- Subjects
General Chemical Engineering ,General Chemistry - Published
- 2022
33. Evaluation of antidiabetic and wound healing properties of ethanol extract of Hedera nepalensis in alloxan-induced diabetic rats
- Author
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Hafiz Muhammad Zubair, Shamsuddin Sultan Khan, Mahrukh, Hafiza Sidra Yaseen, Zafar Iqbal, Mohammad Saleem, Malik Saadullah, Liaqat Hussain, Ikram Ullah Khan, Muhammad Asif, Syed Haroon Khalid, and Memoona Zafar
- Subjects
Antioxidant ,biology ,Traditional medicine ,business.industry ,medicine.medical_treatment ,Plant Science ,Antimicrobial ,biology.organism_classification ,Acute toxicity ,chemistry.chemical_compound ,chemistry ,In vivo ,Alloxan ,Maceration (wine) ,Medicine ,Hedera nepalensis ,business ,Wound healing - Abstract
Natural products are known to control diabetes and its associated complications. Leaves of Hedera nepalensis are used to cure diabetes in the folklore system of medicines. The present study was designed to evaluate the in vivo antidiabetic and wound healing potentials of ethanol extract of Hedera nepalensis. Ethanolic extract (Hn.Cr) was prepared by maceration and was chemically characterized by HPLC methods. Antioxidant and antimicrobial properties were assessed in three in vitro models. Toxicity profile of Hn.Cr was evaluated in acute toxicological studies. Alloxan-induced diabetes model was used to access the antidiabetic attributes of Hn.Cr. Excision wound healing model was used to access the wound healing potential of Hn.Cr in alloxan-induced diabetic rats. HPLC analysis of Hn.Cr revealed the presence of phenolic and flavonoids compounds. Data of in vitro antioxidant assays showed that Hn.Cr has moderate radical scavenging potential. In vitro antimicrobial testing results revealed that Hn.Cr was active against bacterial strains (Gram +ve and Gram -ve) while no activity was observed against fungal strains used in the current study. Acute toxicity study results showed that extract was safe up to the dose of 5000 mg/kg. In vivo antidiabetic study revealed that Hn.Cr significantly (p
- Published
- 2022
34. Raphanus sativus Seeds Oil Arrested in vivo Inflammation and Angiogenesis through Down-regulation of TNF-α
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Ikram Ullah Khan, Tahir Ali Chohan, Hafiz Muhammad Yousaf, Liaqat Hussain, Hafiza Sidra Yaseen, Malik Saadullah, Mohammed Saleem, Muhammad Azam Tahir, Mahrukh, Muhammad Umair Yousaf, Muhammad Usman Shamas, Raghdaa Al Zarzour, and Muhammad Asif
- Subjects
ABTS ,Antioxidant ,DPPH ,medicine.medical_treatment ,Pharmaceutical Science ,Inflammation ,Pharmacology ,In vitro ,chemistry.chemical_compound ,Chorioallantoic membrane ,chemistry ,In vivo ,medicine ,Tumor necrosis factor alpha ,medicine.symptom ,Biotechnology - Abstract
Background: Raphanus sativus is traditionally used as an anti-inflammatory agent. Objectives: The current study was designed to explore the in vivo anti-inflammatory and antiangiogenic properties of Raphanus sativus seeds oil. Methods: Cold press method was used for the extraction of oil (RsSO) and was characterised by using GC-MS techniques. Three in vitro antioxidant assays (DPPH, ABTS and FRAP) were performed to explore the antioxidant potential of RsSO. Disc diffusion methods were used to study in vitro antimicrobial properties. In vivo anti-inflammatory properties were studied in both acute and chronic inflammation models. In ovo chicken chorioallantoic membrane assay was performed to study antiangiogenic effects. Molecular mechanisms were identified using TNF-α ELISA kit and docking tools. Results: GC-MS analysis of RsSO revealed the presence of hexadecanoic and octadecanoic acid. Findings of DPPH, ABTS, and FRAP models indicated relatively moderate radical scavenging properties of RsSO. Oil showed antimicrobial activity against a variety of bacterial and fungal strains tested. Data of inflammation models showed significant (p < 0.05) anti-inflammatory effects of RsSO in both acute and chronic models. 500 mg/kg RsSO halted inflammation development significantly better (p < 0.05) as compared with lower doses. Histopathological evaluations of paws showed minimal infiltration of inflammatory cells in RsSO-treated animals. Findings of TNF-α ELSIA and docking studies showed that RsSO has the potential to down-regulate the expression of TNF-α, iNOS, ROS, and NF-κB respectively. Moreover, RsSO showed in vivo antiangiogenic effects. Conclusion: Data of the current study highlight that Raphanus sativus seeds oil has anti-inflammatory, and antiangiogenic properties and can be used as an adjunct to standard NSAIDs therapy which may reduce the dose and related side effects.
- Published
- 2022
35. Review of: '[Commentary] The Polyfollicular Anovulatory Androgenic Syndrome: A New Label for an Old Syndrome'
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Liaqat Hussain
- Published
- 2022
36. Recent Progress in Flow Control and Heat Transfer Enhancement of Impinging Sweeping Jets Using Double Feedback Fluidic Oscillators: A Review
- Author
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Liaqat Hussain and Muhammad Mahabat Khan
- Subjects
Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics - Abstract
Jet impingement finds an important role in several industrial applications which require high thermal-hydraulic performance of heat exchange systems. The self-exciting sweeping jets produced by fluidic oscillators provide superior thermal and hydraulic performance as compared to plain jets. The fluidic oscillator increases the jet impingement area on the target surface by maintaining a steady and controlled oscillatory flow. The continuous advancements in the design improvement of fluidic oscillators have led to further enhancements of heat and fluid flow characteristics. In this context, double feedback fluidic oscillators have shown promising jet flow control features and jet impingement cooling characteristics. Therefore, the current study has been devoted to analyzing and assessing the recent research progress in design improvements of double feedback fluid oscillators and the augmentation of thermal-hydraulic characteristics of impinging sweeping. Moreover, the variations in the heat transfer and hydraulic performance of impinging jets for different shapes of target surfaces have been comprehensively examined. In the end, research gaps for future work have been highlighted.
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- 2022
37. Effect of sodium alginate supplementation on weight management and reproductive hormones in polycystic females
- Author
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Faiza Manzoor, Mahr-Un Nisa, Abdul Shakoor, Liaqat Hussain, Abid Mahmood, and Anam Younas
- Subjects
Adult ,Blood Glucose ,Dietary Fiber ,Alginates ,Insulins ,Estrogens ,General Medicine ,Lipids ,Metformin ,Body Mass Index ,Young Adult ,Dietary Supplements ,Humans ,Hypoglycemic Agents ,Female ,Testosterone ,Obesity ,Lipoproteins, HDL ,Food Science ,Polycystic Ovary Syndrome - Abstract
Dietary fiber is getting attention these days due to its tendency to improve the reproductive performance in human beings. Sodium alginate (SA) is one of the natural dietary fibers. The present study aimed to evaluate the effect of SA on serum insulin, blood sugar, lipid profile, estrogen and testosterone in polycystic (PCOS) females. A single
- Published
- 2022
38. Correction to Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies
- Author
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Asiya Bashir, Muhammad Asif, Malik Saadullah, Mohammad Saleem, Syed Haroon Khalid, Liaqat Hussain, Ikram Ullah Khan, Hafiza Sidra Yaseen, Hafiz Muhammad Zubair, Muhammad Usman Shamas, Raghdaa Al Zarzour, and Tahir Ali Chohan
- Subjects
General Chemical Engineering ,General Chemistry - Published
- 2022
39. Effects of
- Author
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Anam, Younas, Liaqat, Hussain, Arham, Shabbir, Muhammad, Asif, Musaddique, Hussain, and Faiza, Manzoor
- Abstract
Polycystic ovarian syndrome is a multidisciplinary endocrinopathy of reproductive-aged women that provokes insulin resistance, hyperandrogenism, cardiovascular problems, obesity, and menstrual complications. The present study was designed to investigate the effectiveness of ethanolic extract of
- Published
- 2022
40. Effects of Fagonia indica on Letrozole-Induced Polycystic Ovarian Syndrome (PCOS) in Young Adult Female Rats
- Author
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Anam Younas, Liaqat Hussain, Arham Shabbir, Muhammad Asif, Musaddique Hussain, and Faiza Manzoor
- Subjects
Complementary and alternative medicine ,Article Subject ,food and beverages - Abstract
Polycystic ovarian syndrome is a multidisciplinary endocrinopathy of reproductive-aged women that provokes insulin resistance, hyperandrogenism, cardiovascular problems, obesity, and menstrual complications. The present study was designed to investigate the effectiveness of ethanolic extract of Fagonia indica in letrozole-induced PCOS young adult female rats. HPLC was carried out to find the phenolic and flavonoid content of the ethanolic extract of Fagonia indica. Twenty-five female rats were taken and initially divided into two groups: group I (control group) and group II (PCOS group). PCOS was induced by letrozole given orally by gavage. Body weight was recorded weekly and vaginal cytology was analyzed daily. After induction of disease, the PCOS group is further divided into four groups (n = 5): group II (positive control with PCOS), group III (metformin 20 mg/kg treated group), group IV (ethanolic extract of Fagonia indica 500 mg/kg treated group), and group V (metformin plus Fagonia extract). At the end of experimental period, the blood sample of each rat was collected and serum was separated by centrifugation. Afterwards hormonal analysis, lipid profile and liver functioning tests were performed. Ovaries were removed and preserved for histopathological findings while the liver of each rat was stored for the determination of antioxidant potential assessment. Fagonia indica was found to possess quercetin as one of the major flavonoid phytoconstituents. The plant extract exhibited its beneficial effects by restoring hormonal balance, lipid profile, and liver functioning markers. Treatment with F. indica reduced body weight, resolved ovarian cysts, and showed positive effects on follicular growth. Treatment with plant also increased the levels of antioxidant enzymes. This study validates the potential of Fagonia indica for the amelioration of metabolic, as well as, hormonal disturbances that occurred in PCOS.
- Published
- 2022
- Full Text
- View/download PDF
41. Verapamil attenuates oxidative stress and inflammatory responses in cigarette smoke (CS)-induced murine models of acute lung injury and CSE-stimulated RAW 264.7 macrophages via inhibiting the NF-κB pathway
- Author
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Ximei Wu, Musaddique Hussain, Shahzada Khurram Syed, Malik Saadullah, Ali M. Alqahtani, Taha Alqahtani, Afaf Aldahish, Mobeen Fatima, Saira Shaukat, Liaqat Hussain, Qurratulain Jamil, Imran Mukhtar, Kashif-ur-Rehman Khan, and Ling-Hui Zeng
- Subjects
Pharmacology ,Lipopolysaccharides ,Respiratory Distress Syndrome ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Macrophages ,Acute Lung Injury ,NF-kappa B ,General Medicine ,Cigarette Smoking ,Disease Models, Animal ,Mice ,Oxidative Stress ,Verapamil ,Tobacco ,Animals ,Lung - Abstract
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), severe form of ALI, are characterized by overwhelming of lung inflammation, and no treatment is currently available to treat ALI/ARDS. Cigarette smoke (CS) is one of the prime causes to induce ALI/ARDS via oxidative stress. Despite extensive research, no appropriate therapy is currently available to treat ALI/ARDS. Hence, new potential approaches are needed to treat ALI/ARDS. Consequently, this project was designed to explore the protective effects of verapamil against CS-induced ALI by in vivo and in vitro method. In vivo data obtained from respiratory mechanics, pulmonary morphometric analyses and lung histopathology revealed that verapamil dose-dependently and strikingly decreased the lung weight coefficient, attenuated the albumin exudation into lungs, minimized the infiltration of macrophages and neutrophils into lungs, reduced the pro-inflammatory cytokines (tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and keratinocyte chemoattractant (KC)) production, and improved the hypoxemia and lung histopathological changes. Similarly, verapamil also reduced the production of TNF-α, IL-6 and KC from cigarette smoke extract (CSE)-stimulated RAW 264.7 macrophage. Importantly, verapamil dose-dependently and remarkably suppressed the CS-induced oxidative stress via not only reducing the myeloperoxidase (MPO) activity of lungs, total oxidative stress (TOS) and malondialdehyde (MDA) content in the lungs and supernatant of RAW 264.7 macrophage but also improving total antioxidant capacity (TAC) and superoxide dismutase (SOD) production. Finally, verapamil strikingly decreased the NF-κB expression both in in vivo and in vitro models. Hence, verapamil has positive therapeutic effects against CS-induced ALI via suppressing uncontrolled inflammatory response, oxidative stress and NF-κB p65 signaling.
- Published
- 2021
42. Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
- Author
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Malik Saadullah, Sania Arif, Liaqat Hussain, Muhammad Asif, and Umair Khurshid
- Subjects
Chemical Health and Safety ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Toxicology - Abstract
The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration ( P < .001) while MDA showed significant decrease ( P < .05). The AChE level was significantly reduced ( P < .05) at 500 mg/kg while neurotransmitters were significantly improved ( P < .001) in a dose-dependent fashion. The ELISA results showed significant ( P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD.
- Published
- 2022
43. Hyperthermia Selectively Destabilizes Oncogenic Fusion Proteins
- Author
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Lingfang Wang, Hongyan Li, Liaqat Hussain, Chih-Hung Hsu, Shih-Hwa Chiou, Yasumitsu Ogra, Hua Naranmandura, Haiyan Lou, Vasilis Vasliou, Chang Yang, Ming Hua Ge, Kao-Jung Chang, Mikael Björklund, Yinjun Lou, Clayton A. Smith, Qian Qian Wang, Hao Chen, Jie Sun, Jiebo Lin, Yong Zhu, Yasen Maimaitiyiming, Li Ya Ma, Eric Tse, Jin Zhou, Hongzhe Sun, Yi Ming Shao, Xiaoxia Li, Jinfeng Liu, Ping Huang, Hong-Hu Zhu, Yuan Huang, Jie Jin, Yan Fang Zhang, Ying Huang, Peng-Fei Xu, Hao Ying Hua, Feng-Lin Cao, Xiaodong Cheng, and Na Bu
- Subjects
Acute promyelocytic leukemia ,Hyperthermia ,biology ,Oncogene Proteins, Fusion ,Chemistry ,Mutant ,SIAH2 ,Tretinoin ,General Medicine ,Hyperthermia, Induced ,medicine.disease ,Fusion protein ,Article ,Ubiquitin ligase ,Nuclear receptor ,Leukemia, Promyelocytic, Acute ,In vivo ,medicine ,biology.protein ,Cancer research ,Humans ,neoplasms ,Research Articles - Abstract
The PML/RARα fusion protein is the oncogenic driver in acute promyelocytic leukemia (APL). Although most APL cases are cured by PML/RARα-targeting therapy, relapse and resistance can occur due to drug-resistant mutations. Here we report that thermal stress destabilizes the PML/RARα protein, including clinically identified drug-resistant mutants. AML1/ETO and TEL/AML1 oncofusions show similar heat shock susceptibility. Mechanistically, mild hyperthermia stimulates aggregation of PML/RARα in complex with nuclear receptor corepressors leading to ubiquitin-mediated degradation via the SIAH2 E3 ligase. Hyperthermia and arsenic therapy destabilize PML/RARα via distinct mechanisms and are synergistic in primary patient samples and in vivo, including three refractory APL cases. Collectively, our results suggest that by taking advantage of a biophysical vulnerability of PML/RARα, thermal therapy may improve prognosis in drug-resistant or otherwise refractory APL. These findings serve as a paradigm for therapeutic targeting of fusion oncoprotein–associated cancers by hyperthermia. Significance: Hyperthermia destabilizes oncofusion proteins including PML/RARα and acts synergistically with standard arsenic therapy in relapsed and refractory APL. The results open up the possibility that heat shock sensitivity may be an easily targetable vulnerability of oncofusion-driven cancers. See related commentary by Wu et al., p. 300.
- Published
- 2020
44. Phenylarsine Oxide Can Induce Degradation of PLZF-RARα Variant Fusion Protein of Acute Promyelocytic Leukemia
- Author
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Yasen Maimaitiyiming, Hua Naranmandura, Qian Qian Wang, Li De Su, and Liaqat Hussain
- Subjects
Acute promyelocytic leukemia ,Oncogene Proteins, Fusion ,medicine.medical_treatment ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Arsenicals ,03 medical and health sciences ,chemistry.chemical_compound ,Leukemia, Promyelocytic, Acute ,immune system diseases ,medicine ,Humans ,Idarubicin ,Phenylarsine oxide ,Arsenic trioxide ,neoplasms ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,Chemotherapy ,Ubiquitin proteasome ,Chemistry ,General Medicine ,medicine.disease ,Fusion protein ,Apoptosis ,Cancer research ,HeLa Cells ,medicine.drug - Abstract
PLZF-RARα is the second most frequent variant acute promyelocytic leukemia (APL) fusion protein that ranks after PML-RARα in APL. However, PLZF-RARα is resistant to current front line APL treatments including all transretinoic acid (ATRA), arsenic trioxide (ATO), and chemotherapy (i.e., Idarubicin). Herein, we for the first time report that phenylarsine oxide (PAO) could effectively induce PLZF-RARα variant fusion protein degradation through ubiquitin proteasome degradation pathway by apoptosis, which indicates that PAO might be a potential candidate for the treatment of PLZF-RARα variant APL. Given that, this study highlights the potential benefit of arsenic-organometallic compound PAO in APL treatment.
- Published
- 2019
45. Heat Transfer Augmentation through Different Jet Impingement Techniques: A State-of-the-Art Review
- Author
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Manzar Masud, Muhammad Mahabat Khan, Zabdur Rehman, Fawad Ahmed, Łukasz Amanowicz, Krzysztof Rajski, and Liaqat Hussain
- Subjects
Technology ,Work (thermodynamics) ,Control and Optimization ,Materials science ,Convective heat transfer ,Astrophysics::High Energy Astrophysical Phenomena ,Nozzle ,Energy Engineering and Power Technology ,Physics::Fluid Dynamics ,jet impingement ,Nanofluid ,Electrical and Electronic Engineering ,Aerospace engineering ,heat transfer enhancement ,Engineering (miscellaneous) ,Jet (fluid) ,Renewable Energy, Sustainability and the Environment ,business.industry ,Heat transfer enhancement ,convective heat transfer ,Heat transfer ,Active cooling ,High Energy Physics::Experiment ,fluid surface interaction ,active cooling ,business ,Energy (miscellaneous) - Abstract
Jet impingement is considered to be an effective technique to enhance the heat transfer rate, and it finds many applications in the scientific and industrial horizons. The objective of this paper is to summarize heat transfer enhancement through different jet impingement methods and provide a platform for identifying the scope for future work. This study reviews various experimental and numerical studies of jet impingement methods for thermal-hydraulic improvement of heat transfer surfaces. The jet impingement methods considered in the present work include shapes of the target surface, the jet/nozzle–target surface distance, extended jet holes, nanofluids, and the use of phase change materials (PCMs). The present work also includes both single-jet and multiple-jet impingement studies for different industrial applications.
- Published
- 2021
46. Integrity of zinc finger motifs in PML protein is necessary for inducing its degradation by antimony
- Author
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Na Bu, Yasen Maimaitiyiming, Yong Fei Lan, Liaqat Hussain, Li Ya Ma, Hua Naranmandura, Rui Hao, Xiao Yang Lu, Qian Qian Wang, Ye Jia Chen, Chao Wang, and Chang Yang
- Subjects
0301 basic medicine ,Antimony ,Oncogene Proteins, Fusion ,Cell Survival ,Mutant ,Biophysics ,chemistry.chemical_element ,Antineoplastic Agents ,Protein degradation ,Promyelocytic Leukemia Protein ,Biochemistry ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,Promyelocytic leukemia protein ,Leukemia, Promyelocytic, Acute ,Cell Line, Tumor ,Humans ,Arsenic trioxide ,Arsenic ,Zinc finger ,030102 biochemistry & molecular biology ,biology ,Metals and Alloys ,Zinc Fingers ,Fusion protein ,030104 developmental biology ,chemistry ,Chemistry (miscellaneous) ,Proteolysis ,biology.protein - Abstract
Antimony (Sb) belongs to the same group as arsenic (As) in the periodic table, and both share similar characteristics. However, Sb2O3 (SbIII) has no methylation capacity, unlike arsenic trioxide (As2O3). In the present study, we determined the effect of SbIII on NB4 cells and found that antimony could induce PML-RARα fusion protein degradation, reorganization of PML-NBs, and NB4 cell differentiation with low cytotoxicity. On the other hand, zinc finger motifs in PML protein are considered to be a key target binding site for arsenic-induced PML-RARα protein degradation. Interestingly, antimony and arsenic lost their ability to degrade PML-RARα fusion protein in NB4 cells following pretreatment with phenanthroline (i.e., chelator of zinc ions), indicating that the integrity of zinc finger motifs in PML-RARα fusion protein is a fundamental condition for inducing the protein's degradation by antimony and arsenic. Moreover, we found that SbIII could not induce mutant PML (e.g., A126V and L218P) solubility change and degradation, similar to As2O3. In contrast, we found that the organic antimony compound phenylstibine oxide (PSO) could induce mutant PML protein degradation. In conclusion, our results indicate that SbIII might also be a promising agent to treat acute promyelocytic leukemia, in the same manner as As2O3.
- Published
- 2019
47. Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco-2 cells
- Author
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Liaqat Hussain, Chang Yang, Yun Wang, Hua Naranmandura, and Yasen Maimaitiyiming
- Subjects
0106 biological sciences ,Aptamer ,medicine.medical_treatment ,Biomedical Engineering ,Bioengineering ,Antineoplastic Agents ,01 natural sciences ,Applied Microbiology and Biotechnology ,Targeted therapy ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,Drug Discovery ,medicine ,Humans ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Chemistry ,Oligonucleotide ,Process Chemistry and Technology ,HEK 293 cells ,SELEX Aptamer Technique ,General Medicine ,Aptamers, Nucleotide ,Molecular biology ,HEK293 Cells ,Cell culture ,Nucleic acid ,Molecular Medicine ,Caco-2 Cells ,Drug Screening Assays, Antitumor ,Colorectal Neoplasms ,DNA ,Systematic evolution of ligands by exponential enrichment ,Biotechnology - Abstract
Aptamers are short, single-stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity, and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high-affinity DNA aptamer to selectively target the colorectal carcinoma Caco-2 cells by using cell-based SELEX approach. After 14 consecutive rounds of selection, aptamer ApC1 was identified. Confocal microscopy results revealed that ApC1 could rapidly internalize into Caco-2 cells but not HEK 293 cells. Moreover, it showed high specificity to Caco-2 cells rather than other cell lines such as 293T, HeLa, MCF-7, HL-60, and NB4. Collectively, our results demonstrated that aptamer ApC1 has high specificity to colorectal carcinoma Caco-2 cells, which could be further applied for targeted therapy of colorectal cancer in future studies.
- Published
- 2018
48. Estimation of Stability Parameters for Wide Body Aircraft Using Computational Techniques
- Author
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Muhammad Ahmad, Taimur Ali Shams, Syed Irtiza Ali Shah, and Zukhruf Liaqat Hussain
- Subjects
Computer science ,XFLR ,stability and control ,02 engineering and technology ,lcsh:Technology ,01 natural sciences ,Stability (probability) ,DATCOM ,010305 fluids & plasmas ,lcsh:Chemistry ,Boeing ,Software ,0203 mechanical engineering ,Deflection (engineering) ,0103 physical sciences ,General Materials Science ,Vortex lattice method ,lcsh:QH301-705.5 ,Instrumentation ,Fluid Flow and Transfer Processes ,020301 aerospace & aeronautics ,lcsh:T ,business.industry ,Angle of attack ,Process Chemistry and Technology ,General Engineering ,Aerodynamics ,Structural engineering ,lcsh:QC1-999 ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,aerodynamic coefficients ,Commercial aviation ,lcsh:Engineering (General). Civil engineering (General) ,business ,lcsh:Physics ,United States Air Force Stability and Control Digital DATCOM - Abstract
In this paper, we present the procedure of estimating the aerodynamic coefficients for a commercial aviation aircraft from geometric parameters at low-cruise-flight conditions using US DATCOM (United States Data Compendium) and XFLR software. The purpose of this research was to compare the stability parameters from both pieces of software to determine the efficacy of software solution for a wide-body aircraft at the stated flight conditions. During the initial phase of this project, the geometric parameters were acquired from established literature. In the next phase, stability and control coefficients of the aircraft were estimated using both pieces of software in parallel. Results obtained from both pieces of software were compared for any differences and the both pieces of software were validated with analytical correlations as presented in literature. The plots of various parameters with variations of the angle of attack or control surface deflection have also been obtained and presented. The differences between the software solutions and the analytical results can be associated with approximations of techniques used in software (the vortex lattice method is the background theory used in both DATCOM and XFLR). Additionally, from the results, it can be concluded that XFLR is more reliable than DATCOM for longitudinal, directional, and lateral stability/control coefficients. Analyses of a Boeing 747-200 (a wide-body commercial airliner) in DATCOM and XFLR for complete stability/control analysis including all modes in the longitudinal and lateral directions have been presented. DATCOM already has a sample analysis of a previous version of the Boeing 737, however, the Boeing 747-200 is much larger than the former, and complete analysis was, therefore, felt necessary to study its aerodynamics characteristics. Furthermore, in this research, it was concluded that XFLR is more reliable for various categories of aircraft alike in terms of general stability and control coefficients, and hence many aircraft can be dependably modeled and analyzed in this software.
- Published
- 2021
49. Involvement of PML-I in reformation of PML nuclear bodies in acute promyelocytic leukemia cells by leptomycin B
- Author
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Hua Naranmandura, Qian Qian Wang, Yi Ming Shao, Li De Su, Wei Zhong Chen, Na Bu, Chao Wang, Rui Hao, Li Ya Ma, Liaqat Hussain, and Xiao Yang Lu
- Subjects
0301 basic medicine ,Acute promyelocytic leukemia ,Oncogene Proteins, Fusion ,viruses ,Primary Cell Culture ,Antineoplastic Agents ,Chromosomal translocation ,Promyelocytic Leukemia Protein ,Toxicology ,03 medical and health sciences ,Promyelocytic leukemia protein ,chemistry.chemical_compound ,0302 clinical medicine ,Arsenic Trioxide ,Leukemia, Promyelocytic, Acute ,Cell Line, Tumor ,medicine ,Humans ,Protein Isoforms ,Arsenic trioxide ,Nuclear export signal ,Cell Nucleus ,Pharmacology ,biology ,Chemistry ,food and beverages ,virus diseases ,Drug Synergism ,medicine.disease ,Fusion protein ,Cell biology ,Leukemia ,030104 developmental biology ,Cytoplasm ,030220 oncology & carcinogenesis ,Proteolysis ,embryonic structures ,Fatty Acids, Unsaturated ,Leukocytes, Mononuclear ,biology.protein ,Drug Screening Assays, Antitumor - Abstract
Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation between chromosomes 15 and 17, t(15;17), resulting in the expression of PML-RARα fusion protein, which disrupts the normal PML nuclear bodies (PML-NBs) to micro-speckled pattern, leading to loss of their original functions. Moreover, reformation of PML-NBs in APL by arsenic is considered as one of the important step for APL treatment. Leptomycin B (LMB), a nuclear export inhibitor, is commonly used to inhibit the proteins exporting from the nucleus to the cytoplasm. In the present study, we found that LMB could induce the reformation of PML-NBs in leukemia NB4 cells as well as in APL blast cells from the patients, implying that nuclear shuttle proteins might be involved in the reformation of PML-NBs. Herein, we further found that LMB totally lost the ability to induce PML-NBs reformation when the endogenous PML gene was knocked out, indicating that endogenous PML protein is probably involved in the reformation of PML-NBs. More interestingly, among all PML isoforms (i.e., seven isoforms), reformation of PML-NBs was only observed when co-transfection of PML-RARα with PML-I after LMB treatment. Similarly, deletion of nuclear export signal (NES) of PML-I could also reform PML-NBs, suggesting that the protein level of endogenous PML-I in nucleus is important for the reformation of PML-NBs that interfered by PML-RARα fusion protein. Additionally, LMB has synergistic effect with iAsIII on enhancing PML-RARα fusion protein degradation, and it might provide new insight into APL treatment at clinical level in the near future.
- Published
- 2019
50. Analgesic, anti-inflammatory and antipyretic activity of Salvia moorcroftiana
- Author
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Liaqat, Hussain, Muhammad Sajid Hamid, Akash, Noorul, Ain, and Muhammad Imran, Qadir
- Subjects
Analgesics ,Antipyretics ,Dose-Response Relationship, Drug ,Fever ,Plant Extracts ,Phytochemicals ,Anti-Inflammatory Agents ,Saccharomyces cerevisiae ,Carrageenan ,Mice ,Animals ,Edema ,Salvia ,Pain Measurement - Abstract
Salvia moorcroftiana is an herbaceous plant commonly known as "Kallijari" in Pakistan and belongs to the family Lamiaceae. This study was carried out to evaluate its scientific base for its traditional use in pain, fever and inflammation. The powdered plant was extracted by the method of cold maceration using aqueous methanol (70:30) as solvents. Hot plate, flick tail and acetic acid induced writhing test were utilized for analgesic assessment. Anti-inflammatory activity was evaluated by carageenan-induced mice paw edema. Brewer's induced pyrexia was used for the evaluation of antipyretic activity. Non-significant (p0.01) results as compared to the standard were obtained in all experiments. It was evident from acute toxicity study that plant was non-toxic in nature. It is concluded from the study that plant had the potential to be safely used for pain, fever and inflammation.
- Published
- 2017
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