Search

Your search keyword '"Liao, Xiaowen"' showing total 43 results
Start Over Author "Liao, Xiaowen"
43 results on '"Liao, Xiaowen"'

2. Coupling Dynamics of Complex Electromechanical System

Author

He, Zhouhong, Liao, Xiaowen, Kacprzyk, Janusz, Series Editor, Pal, Nikhil R., Advisory Editor, Bello Perez, Rafael, Advisory Editor, Corchado, Emilio S., Advisory Editor, Hagras, Hani, Advisory Editor, Kóczy, László T., Advisory Editor, Kreinovich, Vladik, Advisory Editor, Lin, Chin-Teng, Advisory Editor, Lu, Jie, Advisory Editor, Melin, Patricia, Advisory Editor, Nedjah, Nadia, Advisory Editor, Nguyen, Ngoc Thanh, Advisory Editor, Wang, Jun, Advisory Editor, Sugumaran, Vijayan, editor, Xu, Zheng, editor, and Zhou, Huiyu, editor

Published
2021
Full Text
View/download PDF

8. Factors Associated with Immediate Antiretroviral Therapy Initiation Among Newly Diagnosed People Living with HIV in Guangdong Province, China.

Author

Huang, Zhaoqian, Liu, Jun, Lin, Kaihao, Yang, Fang, Yan, Yao, Xie, Yingqian, Tan, Zhimin, Liu, Qicai, Li, Junbin, Wang, Lihua, Zhou, Yi, Yao, Gang, Huang, Shanzi, Ye, Chenglong, Cen, Meixi, Liao, Xiaowen, Xu, Lu, Zhang, Chi, Yan, Yubin, and Huang, Lin

Subjects
HIV infection transmission, DIAGNOSIS of HIV infections, HIV infections, SOCIAL support, CONFIDENCE intervals, ATTITUDE (Psychology), SELF-evaluation, ANTIRETROVIRAL agents, SOCIAL stigma, PSYCHOLOGICAL tests, PEARSON correlation (Statistics), AIDS serodiagnosis, RISK perception, SEVERITY of illness index, QUESTIONNAIRES, CENTER for Epidemiologic Studies Depression Scale, MENTAL depression, CHI-squared test, DESCRIPTIVE statistics, CD4 lymphocyte count, INTENTION, LOGISTIC regression analysis, DATA analysis software, ODDS ratio, MARITAL status, PSYCHOLOGY of HIV-positive persons, EARLY medical intervention, LONGITUDINAL method, EDUCATIONAL attainment
Abstract

The article discusses a study which examined factors associated with immediate antiretroviral therapy (ART) initiation among newly diagnosed people living with HIV (PLHIV) aged 18 years and above between May 2018 and June 2019 at six study sites in Guangdong Province, China. Topics include characteristics of the study participants, factors associated with immediate ART initiation among newly diagnosed PLHIV in Guangdong Province, and limitations of the study.

Published
2023
Full Text
View/download PDF

9. Robust Speed Control of Magnetic Drive-Trains With Low-Cost Drives

Author

Liao, Xiaowen, Bingham, Chris, and Smith, Tim

Abstract

The paper presents a methodology to improve the operating robustness of low-cost magnetic drive-train (MDT) systems in which load-side sensing is not a preferred option for addressing pole-slipping and variable torsional stiffness issues. Firstly, through dynamically analysing the relative displacement angle between both sides of the MDT (resulting from the developed electromagnetic- and load-torque), the paper offers an operating criteria using the inertia ratio, electromagnetic- and load-torque excitations to prevent the MDT from pole-slipping. Subsequently, the relationship between controller parameters and dominant/resonant poles of closed-loop MDT control system, is discussed. It is shown that controller parameters for MDTs to accommodate a wide range of torsional stiffness variations can be determined from natural frequencies that are bounded by operating constraints. Using the presented principles, desired performance with respect to speed reference tracking and load-torque disturbance accommodation can be achieved by simply determining the natural frequency of the dominant pole. Simulation studies and experimental measurements on a custom MDT test facility are used to underpin the efficacy of the proposed analysis and design techniques.

Published
2024
Full Text
View/download PDF

18. Servo Control of Drive-Trains Incorporating Magnetic Couplings.

Author

Liao, Xiaowen, Bingham, Chris, Zolotas, Argyrios, Zhang, Qinghua, and Smith, Tim

Subjects
*MAGNETIC coupling, *MAGNETIC control, *TORQUE, *DYNAMIC models, *TORQUE control
Abstract

This article presents a high performance and low-cost design methodology for the servo control of magnetic drive-trains (MDTs) operating in direct drive mode. For the first time, this article considers using sensitivity peaks to analyze the robustness and stability of MDT control systems. Initially, through analysis of a dynamic model, the key spring characteristic parameters with respect to operating points, are developed. It is also shown that a wider dynamic performance envelope can be achieved by linearizing the MDT model at around 60 $\%$ –80 $\%$ of the maximum coupling torque, as opposed to traditional linearization under zero torque conditions. Subsequently, this article exploits the spring characteristics for a design methodology based on the $\text{H}_\infty$ mixed sensitivity approach to determine suitable control parameters. Following this, the maximum exogenous load-torque disturbance and speed reference that will not induce pole-slipping can be determined. Finally, preferential position reference profiles and optimal gains for position controllers are given to prevent demand-induced speed oscillations. The proposed methodologies are validated through simulation and experimental studies. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

22. Leptobrachella shangsiensis Chen & Liao & Zhou & Mo 2019, sp. nov

Author

Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, and Mo, Yunming

Subjects
Amphibia, Leptobrachella, Megophryidae, Animalia, Biodiversity, Anura, Chordata, Taxonomy, Leptobrachella shangsiensis
Abstract

Leptobrachella shangsiensis sp. nov. Figs. 2���3 Holotype. NHMG1704002, adult male (Fig. 2 A���F), collected at Shiwandashan National NR, Guangxi Province, China (22.455�� N, 107.048�� E; alt. 500 m). Collected on 26 April 2017 by Weicai Chen, Yunming Mo, Xiaowen Liao and Shichu Zhou. Paratypes. NHMG1401024���031, NHMG1704003���004, ten adult males, NHMG1401032���034, three adult females, collected at same locality as holotype on 3 Jan. 2014 by Weicai Chen and Yunming Mo. NHMG201604001, one adult female, collected at same locality as holotype on 25 April 2016 by Weicai Chen and Yunming Mo. NHMG1704005, one adult female, collected at same locality as holotype on 26 April 2017 by Weicai Chen and Yunming Mo. Etymology. The specific epithet is in reference to the type locality of Shangsi County, Guangxi Province, China. Suggested English name is Shangsi Leaf Litter Toad. Diagnosis. The specimens were assigned to the genus Leptobrachella on the basis of the following characters: small body size, having an elevated inner metacarpal tubercle, having macro-glands on body (including supraaxillary, femoral and ventrolateral glands), lacking vomerine teeth and having small tubercles on eyelids (Dubois 1980; Lathrop et al. 1998; Delorme et al. 2006). Leptobrachella shangsiensis sp. nov. is distinguished from its congeners by a combination of (1) SVL 24.9���29.4 mm in males (n = 11) and 30.8���35.9 mm in females (n = 5), (2) black supratympanic fold with dark reddish rim, (3) ventrolateral glands distinct, forming a broken line, (4) ventral surface yellowish creamy-white with marble texture, (5) iris copper in the upper and silver in the lower fifth and (6) toe webbing basal and lateral fringes distinct, narrow. Description of holotype. Head width greater than head length (HW / HL = 1.15); snout bluntly rounded in profile and dorsal view, projecting slightly over lower jaw; nostril oval-shaped and closer to tip of snout than eye; canthus rostralis distinct; loreal region distinctly sloping, concave; pupil vertical; diameter of eye less than length of snout (ED / SNT = 0.78); tympanum distinct, rounded, diameter 55% that of eye (TD / ED = 0.55); tympanic rim elevated relative to skin of temporal region; vomerine teeth absent; pineal ocellus absent; vocal sac openings present, located laterally on floor of mouth; tongue large with shadow notch at posterior tip; supratympanic fold distinct, running from corner of eye to axillary gland (Fig. 2 A���D). Forelimbs. Forelimbs thin, slender; tips of fingers rounded, slightly swollen; relative fingers lengths I Hindlimbs. Hindlimbs slender; tips of toes rounded, slightly swollen, similar those of fingers; relative toe lengths I Skin texture and skin glands. Skin on dorsum mostly smooth with numerous tiny tubercles; upper eyelid with small tubercles; ventral skin smooth; pectoral glands visible; femoral glands oval, approximately 1.1 mm in diameter, located on posteroventral surface of thighs, closer to knee than to vent; supra-axillary gland small, oval, approximately 1.1 mm; ventrolateral glands distinct, forming a broken line (Fig. 2 C, D). Color of holotype in life. Dorsum reddish brown with dark brown markings between eyes and scapular region, and scattered some deep reddish tubercles concentrated on upper eyelid and scapular region; black supratympanic fold with dark reddish rim; canthus rostralis with black spots; lateral surfaces of trunk with creamy whitish spots, scattered with about 5 spots; transverse dark-brown bars present on dorsal surface of thighs, tibia, and lower arms; elbows and upper arms orange with light reddish-brown spots on tubercles; ventral surface yellowish creamy-white with marble texture; ventral surfaces of forelimbs and hindlimbs pinkish milk-white with dense whitish speckling; two distinct blackish-grey spots on edge of upper jaw located from snout to area under eye; supra-axillary gland milk-white; femoral and ventrolateral glands white. Iris copper in the upper and silver in the lower fifth (Fig. 2 A���D). Color of holotype in preservative. In preservation, coloration fades to dark grey-brown on dorsum, forelimbs, and hindlimbs. Transverse dark-brown bars are distinct on forelimbs and hindlimbs. Ventral surface is creamywhite. Margin of throat and ventrolateral surfaces of forelimbs and hindlimbs are pale-brown. Macro-glands including supra-axillary glands, femoral and ventrolateral glands are creamy. Measurements of holotype ( mm): SVL 26.8, HL 8.1, HW 9.3, SNT 4.6, ED 3.8, IOD 2.8, TD 2.1, TED 1.3, IN 3.2, EN 1.8, TIB 12.9, FLL 13.4, THL 14.1, PL 12.0, ML 7.0, FEM 1.1. Variation. Measurements of the type series are shown in Table 2. Females (mean SVL 33.3 �� 2.0, n = 5) have larger body size than males (mean SVL 27.4 �� 1.1, n = 11). In life, all specimens have similar coloration (Fig. 3), but in preservation, some specimens have slightly darker brown compared to holotype. Advertisement call. Call descriptions are on the basis of the calls of the holotype, recorded at an ambient temperature of 21.5��C. Calls of L. shangsiensis sp. nov. consisted of a single note, with call duration 64���69 ms (66.03 �� 1.15, n = 30), call repetition rate 3.4 s and intercall interval 184���289 ms (250.53 �� 31.07, n = 30). The dominant frequency was between 5.5���6.5 kHz, but fundamental frequency and harmonics were not clear (Fig. 4). Ecology and distribution. All specimens were found in an evergreen forest in Shiwandashan National NR located at an elevation of 450���550 m (Fig. 5A). Males were calling on rocks within or adjacent to rocky streams at night between 1930���2400 h. Leptobrachella shangsiensis sp. nov. is only known from Shiwandashan National NR. Comparisons. Leptobrachella shangsiensis sp. nov. differs from all other Leptobrachella species based on morphological, molecular and acoustic data. Phylogenetically, L. shangsiensis sp. nov., L. aereus, L. minimus, L. nahangensis, L. nyx, L. pelodytoides, L. pluvialis, L. ventripunctatus and L. wuhuangmontis form a clade. Genetically, among this clade, the smallest genetic distance is 5.9% (L. shangsiensis sp. nov. and L. nyx) and the largest genetic distance is 10.3% (L. shangsiensis sp. nov. and L. ventripunctatus). However, L. shangsiensis sp. nov. can be distinguished from the above-mentioned species by morphological characters. First, L. aereus differs from L. shangsiensis sp. nov. by having a uniformly bronze iris (vs. iris copper in the upper and silver in the lower fifth), the higher frequency calls (6.2���7.9 kHz at 22.4���25.7��C in L. aereus vs. 5.5���6.5 kHz at 21.5��C in the new species) and absent a distinct black supratympanic line and no black spots on flanks (vs. present a black supratympanic line and black spots on flanks). Leptobrachella minimus differs from L. shangsiensis sp. nov. by having no lateral fringes on toes (vs. present narrow lateral fringes on toes) and iris dark gold above and grey below (vs. iris copper in the upper and silver in the lower fifth). Leptobrachella nahangensis differs from L. shangsiensis sp. nov. by having a large body size (male SVL 40.8 mm vs. SVL 24.9���29.4 mm), a uniformly gold iris (vs. iris copper in the upper and silver in the lower fifth) and no lateral fringes on toes (vs. present narrow lateral fringes on toes). Leptobrachella nyx differs from L. shangsiensis sp. nov. by having a relatively large body size (SVL 26.7���32.6 mm in males and 37.0���41.0 mm in females vs. SVL 24.9���29.4 mm in males and 30.8���35.9 mm in females); lateroventral glandular ridge poorly distinct (vs. distinct); dermal ridges under toes rather poorly distinct (vs. distinct); greyish-brown dorsum with dark regularly set rounded spots (vs. reddish brown dorsum); absent dermal fringe along toe V (vs. present); posterior part of thigh with glandular warts (vs. without glandular warts); HW / HL = 0.95 (vs. HW / HL = 1.15); ED / SNT = 1.00 (vs. ED / SNT = 0.78). Leptobrachella pelodytoides differs from L. shangsiensis sp. nov. by having wide webbing on toes (vs. basal toe webbing in L. shangsiensis sp. nov.), poorly distinct dermal ridges under toes (vs. distinct) and present longitudinal skin folds on dorsum (vs. absent). Leptobrachella pluvialis differs from L. shangsiensis sp. nov. by having a relatively small body size (male SVL 21.3���22.3 mm vs. 24.9���29.4 mm), absent dermal fringes on toes (vs. present), dirty white with dark brown marbling on venter (vs. yellowish creamywhite with marble texture) and dark golden iris (vs. iris copper in the upper and silver in the lower fifth). Leptobrachella ventripunctatus differs from L. shangsiensis sp. nov. by having a bicolored iris, copper above and grey-brown below (vs. iris copper in the upper and silver in the lower fifth), absent lateral fringes on toes (vs. present narrow lateral fringes on toes), chest and belly with dark brown spots (vs. yellowish creamy-white ventral surface with marble texture), present longitudinal skin folds on dorsum (vs. absent) and different dominant frequency (6.1���6.4 kHz at 15.0��C vs. 5.5���6.5 kHz at 21.5��C in L. shangsiensis sp. nov.). Leptobrachella wuhuangmontis differs from L. shangsiensis sp. nov. by having a greyish-white ventral surface mixed by tiny white and black dots (vs. yellowish creamy-white ventral surface with marble texture), distinct dark brown blotches on flanks from groin to axilla (vs. about 5 spots on flanks) and rough skin on dorsum body and limbs with skin ridges and dense conical tubercles (vs. nearly smooth dorsal skin with numerous tiny tubercles). Among the genus Leptobrachella, fifteen species (L. baluensis, L. bondangensis, L. brevicrus, L. fusca, L. itiokai, L. juliandringi, L. lateralis, L. mjobergi, L. natunae, L. nokrekensis, L. palmata, L. parva, L. platycephala, L. serasanae and L. tamdil) have no molecular data. Geographically, these species occur in allopatry with L. shangsiensis sp. nov. Leptobrachella baluensis, L. brevicrus, L. itiokai, L. juliandringi, L. mjobergi, L. palmata, L. parva and L. platycephala come from Malaysia; L. bondangensis, L. fusca, L. natunae and L. serasanae from Indonesia; L. lateralis, L. nokrekensis and L. tamdil from northeast India. Morphologically, L. baluensis (male SVL 14.9���15.9 mm), L. bondangensis (male SVL 17.8 mm), L. brevicrus (male SVL 17.1���17.8 mm), L. fusca (male SVL 16.3 mm), L. itiokai (male SVL 15.2���16.7 mm), L. juliandringi (SVL17.0��� 17.2 mm in males and 18.9���19.1 mm in females), L. mjobergi (male SVL 15.7���19.0 mm), L. natunae (male SVL 17.6 mm), L. palmata (male SVL 14.4���16.8 mm), L. parva (SVL15.0��� 16.9 mm in males and 17.8 mm in female) and L. serasanae (female 16.9 mm) have a small body size (vs. SVL 24.9���29.4 mm in males and 30.8���35.9 mm in females in L. shangsiensis sp. nov.). Furthermore, L. baluensis, L. brevicrus, L. bondangensis, L. fusca, L. itiokai, L. juliandringi, L. mjobergi, L. natunae, L. palmata, L. parva and L. serasanae exhibit an arrow-like projection of digital tip (vs. no arrow-like projection of digital tip). Leptobrachella lateralis has no lateral fringes on toes (vs. present narrow lateral fringes on toes). Leptobrachella nokrekensis has reddish orange irises in the upper (vs. irises copper in the upper and silver in the lower fifth). Leptobrachella platycephalus has a large body size (male SVL 35.1 mm vs. SVL 24.9���29.4 mm) and orange red or reddish brown irises (vs. irises copper in the upper and silver in the lower fifth). Leptobrachella tamdil has wide lateral fringes on toes (vs. present narrow lateral fringes on toes) and wide webbing on toes (vs. basal toe webbing). Leptobrachella shangsiensis sp. nov. occurs in sympatry with L. sungi, but L. sungi has a large body size (male SVL 48.3���52.7 mm vs. SVL 24.9���29.4 mm), gold green iris (vs. iris copper in the upper and silver in the lower fifth), absent a distinct black supratympanic line (vs. present) and absent black spots on flanks (vs. present). Leptobrachella laui and liui have a relatively widespread distribution in China. However, L. laui differs from L. shangsiensis sp. nov. by having no black supratympanic fold (vs. present), moderate dermal fringes on fingers (vs. absent), throat transparent pink with brown dusting along anterior margin (vs. throat yellowish creamy-white with marble texture) and head slightly longer than wide (vs. HW / HL = 1.15). Leptobrachella liui differs from L. shangsiensis sp. nov. by having wide lateral fringes on toes (vs. narrow lateral fringes on toes), creamy-white belly with dark brown spots on chest and margins (vs. yellowish creamy-white belly with marble texture), longitudinal ridges under toes interrupted at the articulations (vs. not interrupted) and greyish-brown dorsal coloration in life (vs. brown or reddish-brown dorsal coloration). From the rest 11 known Leptobrachella species from China, L. shangsiensis sp. nov. differs from L. alpinus by having a different dominant frequency (6.7 kHz at 16��C in L. alpinus vs. 5.5���6.5 kHz at 21.5��C in L. shangsiensis sp. nov.) and wide lateral fringes on toes (vs. present narrow lateral fringes on toes in L. shangsiensis sp. nov.). Leptobrachella bourreti exhibits a copper above and golden below iris (vs. iris copper in the upper and silver in the lower fifth), a relatively large body size (male SVL 28.0��� 36.2 mm vs. SVL 24.9���29.4 mm in L. shangsiensis sp. nov.) and poorly distinct dermal ridges under toes (vs. distinct). Leptobrachella eos has a relatively large body size (male SVL 33.1���34.7 mm in L. eos vs. SVL 24.9���29.4 mm in L. shangsiensis sp. nov.), absent black spots on flanks (vs. present), iris orange above and light gold below (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.), absent a black supratympanic line (vs. present) and wide lateral fringes on toes (vs. present narrow lateral fringes on toes in L. shangsiensis sp. nov.). Leptobrachella mangshanensis has a bicolored iris with bright orange upper and greyish cream below (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.) and nearly smooth dorsal skin with some small, orange, tubercles and irregular, dark brown stripes (vs. nearly smooth dorsal skin with numerous tiny tubercles in L. shangsiensis sp. nov.). Leptobrachella maoershanensis has a bicolored iris with orange above and silver below (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.), creamy-white ventral surface with irregular black spots (vs. yellowish creamy-white ventral surface with marble texture) and shagreened dorsal skin small tubercles and longitudinal folds (vs. nearly smooth dorsal skin with numerous tiny tubercles in L. shangsiensis sp. nov.). Leptobrachella oshanensis has a relatively low dominant frequency (4.4���4.6 kHz at 14��C in L. oshanensis vs. 5.5���6.5 kHz at 21.5��C in L. shangsiensis sp. nov.), absent lateral fringes on toes (vs. present narrow lateral fringes on toes in L. shangsiensis sp. nov.) and present wide webbing on toes (vs. basal toe webbing in L. shangsiensis sp. nov.). Leptobrachella purpurus has a relatively low dominant frequency (4.3��� 4.5 kHz at 15��C in L. purpurus vs. 5.5���6.5 kHz at 21.5��C in L. shangsiensis sp. nov.), a dull white venter with indistinct grey dusting (vs. yellowish creamy-white ventral surface with marble texture in L. shangsiensis sp. nov.) and a bicolored iris with orange yellow upper half and sliver white lower half (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.). Leptobrachella tengchongensis has a uniformly dark brown iris (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.), shagreened dorsal skin with small tubercles (vs. nearly smooth dorsal skin with numerous tiny tubercles in L. shangsiensis sp. nov.) and white venter with dark brown blotches (vs. yellowish creamy-white ventral surface with marble texture in L. shangsiensis sp. nov.). Leptobrachella yingjiangensis has a bicolored iris with orange yellow above and sliver white below (vs. iris copper in the upper and silver in the lower fifth in L. shangsiensis sp. nov.), wide lateral fringes on toes (vs. narrow lateral fringes on toes in L. shangsiensis sp. nov.), shagreened dorsal skin with fine, round brown tubercles (vs. nearly smooth dorsal skin with numerous tiny tubercles in L. shangsiensis sp. nov.), ventral surface of body creamy white and scattered with distinct small dark brown flecks on chest and lateral sides of belly (vs. yellowish creamywhite ventral surface with marble texture in L. shangsiensis sp. nov.) and a different dominant frequency (5.7���5.9 kHz at 19��C in L. yingjiangensis vs. 5.5���6.5 kHz at 21.5��C in L. shangsiensis sp. nov.). Leptobrachella yunkaiensis has a pinkish surface of belly with distinct or indistinct light dark brown speckling and black tympanum (vs. yellowish creamy-white belly and brown tympanum in L. shangsiensis sp. nov.) and shagreened dorsal skin with skin ridges and raised warts (vs. nearly smooth dorsal skin with numerous tiny tubercles in L. shangsiensis sp. nov.) Besides the above-mentioned species, the remaining ones (35 of 71 species) belong to different phylogenetic clades, exhibit high genetic variation (ranging from 11.9% to 19.0%) and allopatric distribution. Leptobrachella arayai, L. dringi, L. fritinniens, L. gracilis, L. hamidi, L. heteropus, L. kajangensis, L. kecil, L. marmoratus, L. maurus, L. melanoleucus, L. pictus, L. platycephalus, L. sabahmontanus and L. solus lack supra-axillary and ventrolateral glands (vs. present in L. shangsiensis sp. nov.). The following species, L. applebyi (reddish brown with white specking), L. ardens (reddish brown with, Published as part of Chen, Weicai, Liao, Xiaowen, Zhou, Shichu & Mo, Yunming, 2019, A new species of Leptobrachella (Anura: Megophryidae) from southern Guangxi, China, pp. 67-82 in Zootaxa 4563 (1) on pages 72-79, DOI: 10.11646/zootaxa.4563.1.3, http://zenodo.org/record/2600975, {"references":["Lathrop, A., Murphy, R. W., Orlov, N. & Ho, C. T. (1998) Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19, 253 - 267. https: // doi. org / 10.1163 / 156853898 X 00160"]}

Published
2019
Full Text
View/download PDF

23. Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression

Author

Liao, Xiaowen and Hancock, Robert E.W.

Subjects
Carrier proteins -- Genetic aspects, Genetic transcription -- Regulation, Pseudomonas aeruginosa -- Genetic aspects, Microbial growth -- Genetic aspects, Gene expression -- Analysis, Biological sciences
Abstract

The Pseudomonas aeruginosa penicillin-binding protein 3 (PBP3) homolog (PBP3x) was cloned and analyzed to determine the regulation of pbpB and pbpC gene expression. Analysis of the Pseudomonas aeruginosa PBP3x indicated the presence of an amino acid sequence that was 41%-48% identical to Escherichia coli PBP3. The Pseudomonas aeruginosa PBP3 and PBP3x also exhibited similar functions and were upregulated during the stationary growth phase. Furthermore, the gene encoding PBP3 and PBP3x such as pbpB and pbpC were differentially regulated depending on the growth phase.

Published
1997

24. Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome

Author

Liao, Xiaowen, Charlebois, Isabelle, Ouellet, Catherine, Morency, Marie-Josee, Dewar, Ken, Lightfoot, Jeff, Foster, Jennifer, Siehnel, Richard, Schweizer, Herbert, Lam, Joseph S., Hancock, Robert E.W., and Levesque, Roger C.

Subjects
Pseudomonas aeruginosa -- Genetic aspects, Chromosome mapping -- Research, Genetic markers -- Research, Biological sciences
Abstract

Previous research has located a number of virulence and metabolic genes on the combined physical and genetic map of the Pseudomonas aeruginosa chromosome. However, the use of synthetic probes are likely to result in the location of more genes on the map of the chromosome. To test the validity of this hypothesis, a random survey of the P. aeruginosa genome is conducted. The chromosome is fractionated with two enzymes and PFGE is used to separate the genomic fragments and map a collection of 40 genes. The method is shown to be effective in rapidly mapping the P. aeruginosa gene without linkage analysis.

Published
1996

25. Theloderma kwangsiensis Liu & Hu 1962

Author

Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming, and Huang, Yong

Subjects
Amphibia, Rhacophoridae, Animalia, Biodiversity, Anura, Chordata, Theloderma kwangsiensis, Theloderma, Taxonomy
Abstract

Theloderma kwangsiensis Liu & Hu, 1962 Type locality. Dayaoshan Ranges, Jinxiu County, Guangxi, China. Specimens examined (n=4). NHMG201504001, adult male, was collected at the type locality, the Dayaoshan Ranges, Guangxi, China (24.168397�� N, 110.244343�� E, 1150 m asl). The other three individuals (NHMG201608026, adult male; NHMG20161003, adult male, and NHMG20161101, adult female) were collected at Shiwandashan National Nature Reserve, Guangxi, China (21.844043�� N, 107.891647�� E, 532 m asl). NHMG1504001, NHMG201608026 and NHMG20161003 were found in PVC buckets (diameter 25 cm, height 20 cm) which were used to monitor amphibians. NHMG20161101 was found on a tree approximately 0.5 m above the ground in the evergreen forest. Description. Body dorsoventrally compressed; head length less than head width, head very strongly depressed; snout pointed in dorsal view and profile, projecting beyond margin of the lower jaw; canthus rostral distinct, loreal region sloping; nostrils oval, oblique, nearly reaching the tip of the snout; eye diameter less than snout length; interorbital region concave; interorbital distance slightly less than eye diameter; internarial distance less than eye diameter; pupil horizontal; tympanum distinct, rounded, less than eye diameter, slightly convex relative to skin of temporal region; vomerine teeth in two small oblique groups nearly linking the anterior of the choana; tongue pear-shaped with notch posterior; vocal sac absent. (Table 3; Fig. 5 A���D). Forelimbs moderately robust, relative length of fingers IColoration of T. kwangsiensis in life. The dorsal surface is grass-green with irregular, army-green blotches which consist of large irregular warts studded with small dark red granules. The venter covers cream yellow granules with irregular pale brown spots. The pupil is oval, and the iris is black with cream-yellow reticulations throughout. Ecology. Three individuals of T. kwangsiensis were found in PVC buckets (artificial refugia for monitoring amphibians, diameter= 25 cm, height= 20 cm) on 25 April 2015 (NHMG 201504001), on 25 August 2016 (NHMG 201608026) and on 14 October 2016 (NHMG 20161003). One individual (NHMG 20161101) was found on a tree approximately 0.5 m above the ground in evergreen forest (Fig. 7). Distribution. Currently, we have confirmed that T. kwangsiensis occurs in the Dayaoshan Ranges and Shiwandashan National Nature Reserve. Molecular analyses. The genetic variation between the Dayaoshan Ranges individual and Shiwandashan National Nature Reserve individuals ranged from 0.0���0.6% based on the 16S and 12S DNA fragments sequenced (~2000 bp). Theloderma kwangsiensis nested within T. corticale with high support, and the phylogenetic tree was consistent with Poyarkov et al. (2015), Huang et al. (2017), and Hou et al (2017). The genetic distance between T. kwangsiensis and T. corticale ranged from 0.0���0.6%., Published as part of Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming & Huang, Yong, 2018, Rediscovery of Rhacophorus yaoshanensis and Theloderma kwangsiensis at their type localities after five decades, pp. 484-496 in Zootaxa 4379 (4) on pages 492-495, DOI: 10.11646/zootaxa.4379.4.2, http://zenodo.org/record/1174988, {"references":["Liu, C. C. & Hu S. Q. (1962) A herpetological report of Kwangsi. Acta Zoologica Sinica, 14 (Supplement), 73 - 104.","Poyarkov, N. A. J., Orlov, N. L., Moiseeva, A. V., Pawangkhanant, P., Ruangsuwan, T., Vassilieva, A. B., Galoyan, E. A., Nguyen, T. T. & Gogoleva, S. S. (2015) Sorting out moss frogs: mtDNA data on taxonomic diversity and phylogenetic relationships of the Indochinese species of the genus Theloderma (Anura, Rhacophoridae). Russian Journal of Herpetology, 22, 241 - 280.","Huang, H., Chen, Z., wei, Z., Bu, R. & wu, Z. (2017) DNA barcoding revises a misidentification on mossy frog: new record and distribution extension of Theloderma corticale, Boulenger, 1903 (Amphibia: Anura: Rhacophoridae). Mitochondrial DNA, Part A, DNA Mapping, Sequencing, and Analysis, 29 (2), 273 - 280."]}

Published
2018
Full Text
View/download PDF

26. Rhacophorus yaoshanensis Liu & Hu 1962

Author

Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming, and Huang, Yong

Subjects
Arthropoda, Diplopoda, Chelodesmidae, Polydesmida, Rhacophorus yaoshanensis, Rhacophorus, Animalia, Biodiversity, Taxonomy
Abstract

Rhacophorus yaoshanensis Liu & Hu, 1962 Type locality. Dayaoshan Ranges, Jinxiu County, Guangxi, China. Specimens examined (n=14). All specimens were collected in the Dayaoshan Ranges, Guangxi, China. NHMG1402013, adult female, on a tree approximately 1.5 m above the ground in the evergreen forest (first location: 24.165953�� N, 110.242814�� E, 1218 m asl), collected by Yunming Mo, Zhuqiu Song and Shichu Zhou, at 21:15 h on 26 February, 2014. NHMG14003024, adult male, on leaves of herbaceous plants approximately 0.5 m above the ground in evergreen forest (second location: 24.148056�� N, 110.211111�� E, 1460 m asl), collected by Weicai Chen, Yunming Mo and Shichu Zhou, at 21:43 h on 17 March, 2014. NHMG1503011, adult male, on a bamboo approximately 0.8 m above the ground in evergreen forest (third location: 24.090763�� N, 110.202444�� E, 1417 m asl), collected by Weicai Chen, Yunming Mo and Shichu Zhou, at 22:12 h on 22 March, 2015. NHMG 150401, adult female, and NHMG 150402 -12, 10 adult males, on leaves of herbaceous plants ranging from 0.1 to 0.5 m above the ground in evergreen forest (fourth location: 24.107553�� N, 110.185458�� E, 1372 m asl), collected by Weicai Chen, Yunming Mo and Shichu Zhou, from 21:30 h to 23:45 h, on 14 April, 2015 (Fig. 1). Description. Body dorsoventrally compressed; head length less than head width (HL/HW=0.83); snout pointed in dorsal view and profile, projecting slightly beyond margin of the lower jaw; canthus rostral distinct, loreal region sloping; nostrils oval, oblique, slightly protuberant, and much closer to the tip of snout than eye; eye distance less than snout length (ED/SNT=0.77); interorbital region slightly concave; interorbital distance almost equal to eye diameter (IOD/ED=0.98); internarial space almost equal to eye diameter (IN/EYE=1.03); tympanum distinct, rounded, 50% eye diameter (TD/EYE=0.50), slightly concave relative to the skin of temporal region; pupil horizontal; vomerine teeth in two oblique groups (less than 20�� to horizontal line), closer to choanae than each other; tongue elongated, deeply notched posteriorly; pineal ocellus absent; oval vocal sac opening at base of the jaw; external single subgular vocal sac; supratympanic fold distinct, extending to beyond level of axilla. (Table 2; Fig. 1). Forelimbs moderately robust, relative length of fingers IColoration of R. yaoshanensis in life. The dorsal surface is green, with or without faint green spots; venter is cream without spots; the anterior and posterior surface of thighs, the ventral surface of shanks and the posterior surface of flanks are orange-red without spots; cloacal region is pale grey; throat is grey in males; and the iris is pale yellowish gold with a network of fine dark gold reticulations (Fig. 1). Ecology. R. yaoshanensis specimens were collected from four locations in the Dayaoshan Ranges. Except for the first location, all are close to permanent pools, ranging in area from 5 to 50 m 2. At the fourth location, we found about 40 individuals scattered on leaves of herbaceous plants (Fig. 3). Interestingly, we did not observe vocalizing males or amplexus. One adult female (NHMG 150401) was found, but contained no eggs. However, a female (NHMG 1402013) containing creamy yellow eggs was collected at the first location in February. The advertisement call and tadpole of R. yaoshanensis are unknown. Distribution. Currently, this species is known only from the Dayaohan Ranges, and inhabits evergreen forest above 1100 m elevation. Molecular analyses. Two individuals (NHMG 150404, 150408) were sequenced successfully. Based on our preliminarily phylogenetic analyses, R. yaoshanensis is the sister-species of R. pinglongensis with well-supported values (BBP=1.0) (Fig. 4). The genetic distance between R. yaoshanensis and R. pinglongensis is at the 16S gene fragment examined was 2.0 %., Published as part of Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming & Huang, Yong, 2018, Rediscovery of Rhacophorus yaoshanensis and Theloderma kwangsiensis at their type localities after five decades, pp. 484-496 in Zootaxa 4379 (4) on pages 487-492, DOI: 10.11646/zootaxa.4379.4.2, http://zenodo.org/record/1174988, {"references":["Liu, C. C. & Hu S. Q. (1962) A herpetological report of Kwangsi. Acta Zoologica Sinica, 14 (Supplement), 73 - 104."]}

Published
2018
Full Text
View/download PDF

27. Rediscovery of Rhacophorus yaoshanensis and Theloderma kwangsiensis at their type localities after five decades

Author

Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming, and Huang, Yong

Subjects
Amphibia, Rhacophoridae, Arthropoda, Diplopoda, Chelodesmidae, Polydesmida, Animalia, Biodiversity, Anura, Chordata, Taxonomy
Abstract

Chen, Weicai, Liao, Xiaowen, Zhou, Shichu, Mo, Yunming, Huang, Yong (2018): Rediscovery of Rhacophorus yaoshanensis and Theloderma kwangsiensis at their type localities after five decades. Zootaxa 4379 (4): 484-496, DOI: https://doi.org/10.11646/zootaxa.4379.4.2

Published
2018

29. Apigenin Combined With Gefitinib Blocks Autophagy Flux and Induces Apoptotic Cell Death Through Inhibition of HIF-1α, c-Myc, p-EGFR, and Glucose Metabolism in EGFR L858R+T790M-Mutated H1975 Cells

Author

Chen, ZiSheng, primary, Tian, Dongbo, additional, Liao, Xiaowen, additional, Zhang, Yifei, additional, Xiao, Jinghua, additional, Chen, Weiping, additional, Liu, Qingxia, additional, Chen, Yun, additional, Li, Dongmin, additional, Zhu, Lianyu, additional, and Cai, Shaoxi, additional

Published
2019
Full Text
View/download PDF

36. Characterization of the Pseudomonas aeruginosa penicillin-binding proteins 3 and 3x : gene cloning, expression and role in susceptibility to [beta]-lactam antibiotics

Author

Liao, Xiaowen

Abstract

Two degenerate oligonucleotides primers were synthesized based on the amino acid sequences found in the conserved SXXK and KTG motifs of Escherichia coli high-molecular-weight PBPs and Nesseria gonorrhoeae PBP2. The primers were subsequently used in a PCR amplification experiment using Pseudomonas aeruginosa PAOl chromosomal DNA as the template. Five of the resulting PCR products were cloned and sequenced: two products that translated to sequences with strong homology to E. coli PBP3 and N. gonorrhoeae PBP2 were subsequently used as probes to clone the completepbpB and pbpC genes; the other three PCR products were identified as the homologues of the E. coli sucClsucD, yhhF and cypH gene products. The derived amino acid sequence of pbpB gene had 45.1% identity to that of E. coli PBP3. The downstream sequence of pbpB encoded an amino acid sequence homologous to the E. coli murE gene product. These two genes mapped to the same region of the chromosome as did other cell division genes including ftsA,ftsZ and env A. Analyses of the translated sequence of the pbpC gene revealed that it had 40.7% identity to that of E. coli PBP3. The downstream sequence of pbpC encoded convergently transcribed homologues of the E. coli soxR and Mycobacterium bovis adh gene products. Its upstream sequence, about 370 bp in length, did not resemble any sequences in the GenBank database. The pbpC gene mapped 2 megabase pairs from the pbpB gene on the P. aeruginosa chromosome and apparently was not associated with genes involved in cell division. The upstream sequence of pbpC contained a potential a s recognition site, suggesting that the expression of this gene may be growth or stress regulated. The pbpB and pbpC genes were expressed in E. coli by the T7 RNA polymerase and promoter system. The produced proteins were exported to the cytoplasmic membrane of E. coli cells and bound 3H-penicillin. They had an apparent molecular mass of 60 and 58 kDa respectively, whereas the calculated molecular mass were 63.69 and 61.128 kDa. The N-terminal amino acid sequences of the proteins produced in E. coli were identical to those deduced from the nucleotide sequences of the pbpB and pbpC genes, suggesting that there was no N-terminal processing. The pbpB and pbpC genes were expressed in P. aeruginosa PAO4089 using a broad-host-range vector pUCP27. Results from the minimal inhibitory concentration testing and 3H-penicillin binding competition assays indicated that overproduction of pbpB gene product led to increased resistance to the PBP3-targeted antibiotics aztreonam, cefepime, cefsulodin and ceftazidime whereas the presence of the pbpC gene product in PAO4089 did not have any effect on susceptibility to the tested PBP3- targeted antibiotics. E. colipbpB gene was expressed in PAO4089 using pUCP27. Overproduction of the E. colipbpB product in PAO4089 resulted in increased resistance to aztreonam, cefepime and ceftazidime. The attempt to construct a PBP3-defective mutant using a gene replacement technique was not sucessful. This result could be due to the location of the pbpB gene at the proximal end of an operon containing a cluster of cell division genes, where the placement of a polar mutation would be lethal to the cells. Using the same approach, a PBP3x-defective mutant (strain HCI32) was obtained and confirmed by Southern blot analysis. The PBP profiles of wild type strain HI03 and mutant strain HCI32 were similar, suggesting that the pbpC gene was not visibly expressed under the physiological conditions tested. Furthermore, inactivation of PBP3x did not cause any changes in cell morphology or growth rate, suggesting that pbpC was not required for cell viability under normal laboratory growth conditions.

Published
1996
Full Text
View/download PDF

38. Speed Control of Magnetic Drive-Trains with Pole-Slipping Amelioration

Author

Liao, Xiaowen, Bingham, Chris, Smith, Tim, Liao, Xiaowen, Bingham, Chris, and Smith, Tim

Abstract

The paper introduces new techniques to reduce the potential for pole-slipping induced by control systems and presents a low-cost pole-slipping detection and recovery scheme for magnetic drive-trains (MDTs). For the first time, the paper shows that a combination of electromagnetic and load-torque excitations which individually are not greater than the maximum coupling torque can initiate pole-slipping. For applications where acceleration feedback is unavailable, the motor-side inertia is virtually increased with a tracking differentiator to provide feedback of acceleration. Subse- quently, controller design and parameter optimization are discussed. Experimental measurements on a custom test facility verify the presented principles that low-bandwidth controller designs with low inertia ratios can accommodate a wider range of on-load startup torque and load-torque disturbances without pole-slipping. To address overload issues, a pole-slipping detection method based on the kurtosis of electromagnetic torque and a recovery strategy based on converting the state of pole- slipping into that of on-load startup are presented. Experimental results demonstrate that detecting slip anomalies without load-side information, and recovery from pole-slipping without auxiliary mechanical devices are both feasible.

39. Speed control of drive-train incorporating magnetic coupling

Author

Liao, Xiaowen, Bingham, Chris, Zolotas, Argyrios, Zhang, Qinghua, Smith, Tim, Liao, Xiaowen, Bingham, Chris, Zolotas, Argyrios, Zhang, Qinghua, and Smith, Tim

Abstract

The paper presents a two-degree-of-freedom control method that exploits the spring dynamics of a magnetic coupling for electromechanical torque transfer. Initially, through analysis of a dynamic model and linearisation, it is shown that control parameters designed by previous reported methods can be too aggressive, and that a broader performance envelope can be obtained by linearising at around 75% of pull-out torque. Secondly, the paper presents a method of identifying the spring dynamics without measuring the load-side position of the coupling. Finally, input shaping and the identified parameters are employed to provide a new methodology. The internal model principle is used along with the proposed linearisation scheme to construct a feed-forward/feedback control scheme whose parameters are calculated to provide a desired tracking and disturbance rejection characteristics. Experimental results show excellent reference tracking and load torque disturbance rejection performance, whilst reducing the possibility of poles-lipping due to aggressive control action.

40. The Identification of Load-side Inertia for Magnetic Drive Trains

Author

Liao, Xiaowen, Bingham, Chris, Smith, Tim, Liao, Xiaowen, Bingham, Chris, and Smith, Tim

Abstract

The paper presents an identification method based on adaptive frequency tracking for the realtime identification of load-side inertia of magnetic drive trains encompassing variable torsional stiffness. Firstly, through analyzing the transient response of motor-side speed it is shown that the response incorporates a ramp component and a damped sinusoidal signal with a frequency approximately equals to the resonant frequency. Subsequently, a second order bandpass filter is designed to remove the ramp component and combined with an adaptive notch filter (ANF) forms a fourth order ANF. With the resonant frequency identified by the ANF, the moment of load-side inertia is then estimated. Finally, a numerical implementation method based on automatically calculating the damping ratio along with bandwidth, gain, and sampling frequency, is presented, and an iterative identification procedure is provided to solve problems associated with the limited duration of oscillations existing in the step response. Results show that the proposed methodology identifies the moment of load-side inertia with an accuracy of 3.3%.

41.  Ophiorrhizareflexa (Rubiaceae), a new species from a karst region in Guangxi, China.

Author

Shang C, Xue J, Yang Y, Liao X, Liu Q, and Wu L

Abstract

Ophiorrhizareflexa , a new species from Guangxi, China, is described and illustrated in this study. It is morphologically similar to O.alatiflora due to the branched inflorescence, distylous flowers and the tubular-funnelform corolla with five longitudinal wings. The new species can be distinguished from O.alatiflora by its erect inflorescence, its smaller and equal-sized calyx lobes 0.5-0.7 mm long, its corolla tubes winged to the middle and the wings straight and its strongly reflexed corolla lobes at anthesis. Ophiorrhizareflexa is assessed as least concern (LC) according to IUCN Categories and Criteria., Competing Interests: The authors have declared that no competing interests exist., (Chao Shang, Jun Xue, Yanjie Yang, Xiaowen Liao, Quanru Liu, Lei Wu.)

Published
2024
Full Text
View/download PDF

42.  Mitreolaquanruii (Loganiaceae), a new species from a karst region in Guangxi, China.

Author

Hu R, Liao X, Luo B, Liu C, Nong Y, and Wu L

Abstract

Mitreolaquanruii , a new species from Guangxi, China, is described and illustrated in this study. It is morphologically similar to M.liuyanii because of the terete stems, creeping and branched at the base, the leaves which are pilose on both surfaces and the bilobed capsules with two erect horns. The new species can be distinguished from M.liuyanii by its taller habit, up to 20-50 cm tall, its linear leaves, 4-18 × 0.3-1 cm with acuminate apex and 8-10 pairs of lateral veins, its narrowly triangular stipules, its linear bracts, ca. 1.0 cm long and glabrous and its glabrous calyx. Mitreolaquanruii is temporarily assessed as data deficient (DD) according to IUCN. The habitat of Mitreolaquanruii is extremely fragile. Therefore, this species deserves close attention and protection., Competing Interests: The authors have declared that no competing interests exist., (Renchuan Hu, Xiaowen Liao, Binsheng Luo, Cheng Liu, You Nong, Lei Wu.)

Published
2023
Full Text
View/download PDF

43. Suppression of PI3Kp110β Reduces Cell Viability and Induces Apoptosis in Human Esophageal Cancer.

Author

Pan D, Liao X, Tan S, Wu M, Wang X, Li M, Xu W, Jiang M, and Chen G

Subjects
Apoptosis genetics, Cell Line, Tumor, Cell Proliferation genetics, Cell Survival genetics, Humans, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, RNA pharmacology, bcl-2-Associated X Protein pharmacology, Class I Phosphatidylinositol 3-Kinases metabolism, Esophageal Neoplasms genetics, Esophageal Neoplasms pathology, Sincalide pharmacology
Abstract

Objective: Activation of PI3K pathway has been reported to promote survival, tumorigenicity and metastasis in the esophageal cancer (EC), and the PI3Kp110β (one of PI3K family) contributed to tumorigenesis in types of cancers. However, it is still unclear whether PI3Kp110β effects the progression of EC., Methods: RT-qPCR and western blot were used to detect the expression of PI3Kp110β in the patients with EC and ECA-109 cells transfected with si-PI3Kp110β. CCK-8 and clonogenic assays were performed to analyze the proliferation of ECA-109 cells. Flow cytometry was used to investigate cell cycle and apoptosis of ECA-109 cells., Results: The expression of PI3Kp110β was up-regulated in the patients with EC by online RNA Sep data and RT-qPCR assay. Compared with the ECA-109 cells with si-control, the proliferation was suppressed in the ECA-109 cells with si-PI3Kp110β. The G1 phase of the cells with si-PI3Kp110β was significantly higher than that of cells with si-control. The apoptosis of cells with si-PI3Kp110β was accelerated, compared with that of cells with si-control. The expression of cyclinD1 and Bcl-2 was decreased, while the expression of Bax was increased in the ECA-109 cells with si-PI3Kp110β., Conclusion: Inhibition of si-PI3Kp110β attenuates cell viability and enhances apoptosis in esophageal cancer., (© 2022 by the Association of Clinical Scientists, Inc.)

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results