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Style transfer refers to extracting styles from a specified style image template, and mapping the extracted style features to the content image without destroying the image content. At present, the use of deep learning methods for style transfer is one of the hotspots in the field of image research. This paper implements an image style transfer architecture based on cyclic consistent generative adversarial network, i. e., cyclic consistent confrontation network, and uses densely connected convolutional network to deepen the number of network layers. Test results show that stylized image with the proposed method is more obvious than that of the original cyclic consistent generative adversarial network, and the image quality is improved apparently.

Concurrent programs are commonly used in real-time system software. Verifying the correctness of concurrent programs is an important job to ensure the reliability of real-time system software. The causes of concurrency in a real-time system program include task scheduling and interrupt mechanism, where multi-interruption is an effective means of real-time response to asynchronous events. This paper studies the modeling approach for multi-interruption concurrent programs based on TMSVL (Timed Modeling, Simulation and Verification Language), so as to verify temporal properties of multi-interruption concurrent programs, such as safety and liveness properties. The formal syntax and semantics of multi-interruption concurrent programs is established based on TMSVL, and the correctness and practicability of our approach is demonstrated with a case study.

To examine antigen (Ag)-specific CTL response during anterior chamber associated immune deviation (ACAID).OVA or OVA257-264 peptide was injected into the anterior chamber (AC) of C57BL/6 mice. There were 16 mice in each ACAID group induced with OVA or OVA257-264 peptide. The mice were primed by SC injection with OVA or OVA 257-264 peptide in complete Freund's adjuvant (CFA) on day 7. Ag-specific CD8+ T cells in spleens were analyzed on day 14 using Pentamer H-2K(b)-SIINFEKL(OVA257-264 peptide). IFN-gamma ELISPOT and intracellular granzyme B staining were used to characterize the CTL response. Twelve mice in each group immunized with OVA or OVA257-264 peptide in CFA served as positive controls. Twelve normal mice served as negative controls and 12 receiving injection of CFA as CFA controls for studying the influence of CFA on the Ag-specific CTL response.The results showed that anterior chamber inoculation of OVA or OVA257-264 peptide could induce ACAID as evidenced by an impaired DTH response. The frequency of Ag-specific CD8+ T cells in ACAID mice was not different from that in mice challenged with Ags in CFA only (positive controls). IFN-gamma production by these cells in ACAID mice was not different compared to positive controls. However, Ag-specific CD8+ T cells in ACAID mice failed to secrete granzyme B. Mice challenged only with OVA peptide and CFA also showed a granzyme B negative CD8+ T cell response. Ag-specific CTL response induced by CFA alone was similar with the negative control.These results show that the frequency of Ag-specific CD8+ T cells is not altered during ACAID. The Ag-specific CTL response during ACAID is characterized by the absence of granzyme B expression.

Orthogonal Frequency Division Multiplexing (OFDM) is suitable for high-bit-rate data transmission in multipath environments. Many error-correcting codes have been applied in COFDM to reduce the high bit error rate (BER). Recently, low-density lattice codes (LDLC) attracted much attention. LDLC was proposed by Sommer in 2007, and the performance is very close to Shannon limit with iterative decoding. We applied LDLC in COFDM system and the simulation results showed that the LDLC is effective to improve the BER of the system in multipath environments.

In the wireless network, cooperative diversity has proved itself an effective technology to enhance the performance of system. For the two-user cooperative communication system model, this paper gives a detailed analysis on three kinds of conditional cooperative diversity schemes which include the amplification and forward, decode and forward and coded cooperation; It also shows the simulation model of cooperative communication in the form of graphs, describing the specific cooperative process; and it has a research on the performance of three cooperative diversity communication system with LDPC codes under the conditions of selective cooperation. Simulation results show that: Selective cooperation can reduce the processing complexity of the base station and cooperative users, so that the overall system performance is improved.

An electrical, instrumentation and computer (EIC) integrated system which fully incorporated with micro-processor based programmable controller, distributed control system, data highway and process control computer had been completed for Tangshan Iron and Steel Company new No.l blast furnace. Accordingly, the total system configuration, outline of the automation system and some considerations in system engineering are described.

T lymphocytes have been implicated in the development of endotoxin-induced uveitis (EIU). T-bet is a Th1 cell-specific transcription factor that is involved in differentiation and effector functions. The aim of this study was to investigate kinetics of T-bet expression at the mRNA and protein levels during EIU using real-time PCR and whole-mount immunohistochemistry. Methods A single footpad injection of 200 ¿g of lipopolysaccharide (LPS) was administered to male Wistar rats in order to induce EIU. Clinical changes were followed by slit-lamp examination. The expression of T-bet mRNA in the spleen was evaluated 0, 8, 16, 24, 48, and 96 h after LPS injection using real-time PCR. Immunohistochemistry was performed on the iris whole-mounts as well as on frozen sections of the spleen to evaluate T-bet protein expression. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on the iris whole-mounts to assay apoptotic cells. Results Uveitis was observed in all rats that received LPS. T-bet+ cells and TUNEL+ cells in the iris whole-mounts showed a similar pattern in cell number and distribution and both types of cells were observed at 8 h, significantly increased 24 h, and decreased 48 h after LPS injection. T-bet expression at both the mRNA and protein levels in spleen also paralleled ocular inflammation. It was weakly detectable after 0 h, increased after 8 h (index 1.3, T-bet+ cells OD 17.43±2.15), reached its peak after 24 h (index 4.00, OD 53.52±4.00), and decreased 48 h following LPS injection (index 1.38, OD 25.75±2.45). Conclusions The results show that T-bet expression in both the iris and the spleen, and in apoptotic cells in the iris parallel the severity of intraocular inflammation after systemic LPS administration. These results suggest that T-bet may play a significant role in the dynamics of EIU.

To study the expression and functional characteristics of programmed death-1 (PD-1) and its ligands in the spleens of mice undergoing anterior chamber-associated immune deviation (ACAID). METHODS: ACAID was induced in BALB/c mice by intracameral injection of ovalbumin (OVA). The expression of PD-1 and its ligands in the spleens of ACAID mice was determined by quantitative real-time PCR, Western blotting, and flow cytometry. In vitro proliferation assays, enzyme-linked immunosorbent assays, and adoptive transfer assays were used to investigate the functional characteristics of splenic CD4+PD-1+ T cells of ACAID mice. RESULTS: Both mRNA and protein of PD-1, PD-L1, and PD-L2 were markedly upregulated in the spleens of ACAID mice compared with controls. CD4+PD-1+ T cells from ACAID mice produced large amounts of IL-10 and exhibited in vitro antigen-specific suppressive activity. CD4+PD-1+ T cells from ACAID mice were able to significantly inhibit the antigen-specific, delayed-type hypersensitivity response when adoptively transferred to naive mice. CONCLUSIONS: CD4+PD-1+ T cells from ACAID mice, as regulatory cells, are involved in the induction of antigen-specific suppression in association with enhanced expression of IL-10. CD4+PD-1+ T cells in the murine spleen may represent a substantial population of regulatory T cells possibly responsible for the induction of ACAID after intracameral injection of antigen.

Indoleamine 2,3-dioxygenase (IDO), a rate-limiting enzyme in the tryptophan catabolism, has been shown to play an important role in various forms of immune tolerance. Since anterior chamber associated immune deviation (ACAID) is a systemic immune tolerance elicited by introducing exogenous antigens into the anterior chamber of the eye, we investigated the expression and function of IDO in the development of this ocular tolerance. ACAID was induced in BALB/c mice by an intracameral injection of 50mug ovalbumin (OVA). The IDO expression in the splenocytes during ACAID was determined by fluorescent quantitative real-time PCR, Western blot analysis and immunohistochemistry. The development of ACAID was evaluated by the delayed-type hypersensitivity (DTH) response after intraperitoneal injection of an IDO inhibitor 1-methyl-dl-tryptophan (1-MT). Secretion of IFN-gamma and IL-4 by splenocytes and lymph node cells from the mice treated with or without 1-MT were also evaluated using intracellular cytokine staining. Our results showed that the IDO expression was significantly increased at both mRNA and protein levels following OVA intracameral injection. Inhibition of IDO with 1-MT prevented the development of ACAID, which was indicated by the re-appearance of the OVA-specific DTH response. IL-4 was significantly reduced and IFN-gamma was partially recovered after the treatment of 1-MT. Our study reveal that IDO is up-regulated during ACAID and IDO inhibitor prevents ACAID generation, suggesting that IDO is involved in the development of this immune tolerance.

To assess the expression of T-cell immunoglobulin- and mucin-domain-containing molecule 3 (Tim-3) in the spleens of BALB/c mice undergoing anterior chamber-associated immune deviation (ACAID). ACAID was generated after intracameral (i.c.) injection of ovalbumin (OVA) into BALB/c mice and evaluated by assessing the delayed-type hypersensitivity (DTH) response following a subsequent subcutaneous (s.c.) injection of OVA emulsified in complete Freund's adjuvant (CFA) on Days 0, 3, 7, 14, 21 and 28. Tim-3 mRNA levels were detected using real-time RT-PCR, and the frequency of CD4+Tim-3+ T cells in splenocytes as well as the coexpression of Tim-3 with CD25 on CD4+ T cells were assessed by flow cytometry. A significantly suppressed DTH response was observed on Days 7, 14, 21, and 28, but not on Days 0 and 3 during the development of ACAID. The levels of Tim-3 mRNA and the frequency of CD4+CD25+Tim-3+ T cells in the splenocytes reached a peak on Day 3, declined on Day 7, and returned to basal levels thereafter. A transient upregulation of Tim-3 expression was observed in the early stage of ACAID, suggesting its possible involvement in the development of ACAID

T-cell receptor (TCR) plays an important role in the development of autoimmune diseases. Recently, it was reported that immunization of animals with TCR peptide derived from the pathogenic cells could prevent autoimmune diseases. The aim of this study was to investigate whether vaccination with a synthetic peptide from the hypervariable region of TCR V(beta) 8.3, an experimental autoimmune uveoretinitis (EAU)-associated gene, was able to prevent the disease. METHODS: EAU was induced in Lewis rats by immunization with IRBP R16 peptide emulsified in complete Freund's adjuvant (CFA). The clinical and histological appearances were scored. Delayed type hypersensitivity (DTH) and lymphocyte proliferation were detected. Cytokine levels of aqueous humour, supernatants of cells from spleen and draining lymph nodes were measured by enzyme linked immunosorbent assay (ELISA). Gene expression of TCR V(beta) 8.3 on CD(4)(+) T cells was examined by real time quantitative polymerase chain reaction (PCR). RESULTS: After vaccination, the intraocular inflammation was significantly mitigated, antigen specific DTH and lymphocyte proliferation responses were suppressed, interleukin (IL)-2 in aqueous humour, interferon (IFN)-gamma and IL-2 produced by the spleen and draining lymph node cells were significantly decreased, whereas the production of IL-4 and IL-10 were increased. The response of draining lymph node cells to TCR V(beta) 8.3 peptide was enhanced after vaccination. Inoculation with CFA alone did not affect the severity of EAU and the above parameters. The suppression of EAU was much stronger in the group of four fold inoculations than the group of two fold inoculations. The expression of TCR V(beta) 8.3 gene was significantly reduced in the group of fourfold inoculations. CONCLUSION: Vaccination with the synthetic TCR V(beta) 8.3 peptide could remarkably inhibit the development of EAU.

Turbo codes and low-density parity-check (LDPC) codes both have their advantages and can achieve the Shannon limited performance. The constituent recursive systematic convolutional (RSC) codes in turbo codes are more structural and this lends the encoding problem easier with a shift-register circuit. While the encoding of LDPC codes is performed via matrix multiplication, and this is more complex than it appears for capacity-approaching LDPC codes. On the other hand, the soft-input soft-output BCJR algorithm, or the sub-optimal version of it, used for turbo-decoding is rather complex while sum-product algorithm used for LDPC decoding lends itself to parallel implementation and is computationally simpler. Combining the turbo codes encoding and LDPC decoding, a new scheme based on factor graphs and sum-product algorithm is developed, it can reduce the decoding complexity of turbo codes greatly, and also has some guides in the designing of interleaver and the choosing of RSC constituent codes. Simulation shows the correctness of the scheme.

Turbo codes and low-density parity-check (LDPC) codes both have their advantages and can achieve the Shannon limited performance. Combining the turbo codes encoding and LDPC decoding, a new scheme based on factor graphs and sum-product algorithm is developed in this paper. It can reduce the decoding complexity of turbo codes greatly, and also has some guides in the designing of interleaver and the choosing of recursive systematic convolutional (RSC) constituent codes.

Based on a digital phase ramp feedback technique, a new type of all digital closed loop FOG detection scheme is introduced. After a detailed analysis of its principles and design, a complete FOG signal detection system is developed and set-up. Experiments and tests show that the scheme can solve problems such as scale factor error and dynamic range effectively.