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Summary: Heart failure (HF) is a global concern, particularly HF with preserved ejection fraction (HFpEF), lacking effective treatments. Understanding the differences of metabolic profiles between HFpEF and HFrEF (heart failure with reduced ejection fraction) patients is crucial for therapeutic advancements. In this study, pseudotargeted metabolomics was employed to analyze for disparities of plasma metabolic profiles between HFpEF and HFrEF in two cohorts: discovery (n = 514) and validation (n = 3368). Plasma-free carnitine levels were significant changed in HF patients. A non-linear and U-shaped (for HFpEF) or J-shaped (for HFrEF) association between circulating free carnitine levels and the composite risk of cardiac events were observed. Interestingly, HFpEF patients with low free carnitine (≤40.18 μmol/L) displayed a poorer survival, contrasting with HFrEF where higher levels (≥35.67 μmol/L) were linked to poorer outcomes, indicating distinct metabolism pathways. In conclusion, these findings offer insights into HFpEF metabolic profiles, suggesting potential therapeutic targets.

The open-phase operation of the AC connection line connecting the wind farm and MMC generates negative sequence current in the system, which cause the overcurrent, overvoltage, power fluctuation and other problems. It seriously affects the power transmission and equipment safety. Therefore, it is of great significance to study the negative sequence current suppression scheme suitable for the open-phase operation and master the calculation method of electrical quantities for improving the utilization rate of wind power and ensuring the stability of the system. Based on the negative sequence current relationship between wind farm and MMC, coordinated control schemes to suppress the negative sequence current by wind farm or MMC are proposed. The calculation method of the electrical quantities for two negative sequence current suppression schemes are studied, and their requirements on the voltage and current resistance for equipment of wind farm and MMC are calculated respectively. The accuracy of the calculation method is verified in PSCAD/EMTDC, and it gives the quantitative requirements of the negative sequence control scheme on the tolerance of the system in the specific example.

ObjectiveTo investigate the use of contraceptive methods， and to evaluate maternal healthcare services utilization among women within 2 years in Pudong New Area of Shanghai.MethodsA cross-sectional survey was conducted using a questionnaire for women at different postpartum periods who visited a community health clinic with their children from June to November 2021. Data were analyzed using χ2 test and binary logistic regression.ResultsAmong the 1 946 postpartum women surveyed， 1 934 were either married or cohabiting， and 1 430 had resumed their sexual life. Among women at 4， 6， 12， 18 and 24 months postpartum， the contraceptive prevalence rates （CPR） were 92.34% （193/209）， 92.16% （235/255）， 87.31% （282/323）， 91.95% （297/323） and 90.00% （288/320）， respectively. The modern contraceptive prevalence rates （mCPR） were 87.08% （182/209）， 87.06% （222/255）， 82.04% （265/323）， 83.09% （271/323） and 85.31% （273/320）， respectively， while the unmet contraceptive needs （UMNs） were 7.66% （16/209）， 7.84% （20/255）， 11.46% （37/323）， 6.81% （22/323） and 10.00% （32/320）， respectively. The use rates of long-acting reversible contraceptive （LARC） methods among women at 6， 12， 18 and 24 months postpartum period were 0.39% （1/255）， 2.17% （7/323）， 0.31% （1/323）， and 2.81% （9/320）， respectively. Among all surveyed subjects， 32.17% （626/1 946） had received postpartum contraceptive services only once， while 27.85% （542/1 946） had not received any postpartum contraceptive services. Binary logistic regression analysis indicated that the use of contraceptive methods among postpartum women was associated with whether relevant educational services were received after childbirth and during postpartum home visits （all P

Bamboo is widely used in the construction industry due to its excellent mechanical properties, short production cycle, and environmental concerns. Reconstructing and processing bamboo into biomass-based composite materials that can replace wood alleviates the imbalance between wood supply and demand. This article uses cold press setting and hot press curing molding processes to prepare small samples of Hexagonal Recombinant Bamboo Material (glued HexBam). Through orthogonal analysis, the preparation process parameters, adhesive types, adhesive application amounts, and the relationship between the temperature and time of hot pressing solidification molding and the mechanical properties of glued HexBam were determined. A phenolic adhesive (PF) was used with a dosage of 200 g/m2, a heat setting and molding temperature of 130 °C, and a heat setting molding time of 10 minutes. Under these process conditions, the prepared glued HexBam had a longitudinal compressive strength of 73.8 MPa and a specific strength of 0.0535 N•m/kg, which is superior to ordinary steel.

Drought stress severely impairs common bean production. For facilitating drought-resistance breeding in common bean, molecular markers were identified in a genome-wide level marker–trait association study. A panel of 210 common bean accessions showed large variation in 11 agronomic traits at the adult stage (plant height, pod number per plant, seed number per pod, seed number per plant, seed yield per plant, pod length, harvest index, pod harvest index, days to maturity, hundred-seed weight, and seed yield) under two water conditions. The coefficient of variation ranged from 6.21% for pod harvest index to 51.00% for seed number per plant under well-watered conditions, and from 4.05% for days to maturity to 40.72% for seed number per plant under drought stress. In a genome-wide association study, 119 quantitative-trait loci were associated with drought resistance, including 41 adjacent to known loci. Among these loci, 12 were found to be associated with at least two traits. Three major loci were identified at Pv01 and Pv02. A set of candidate genes were found that encode MYBs, AREBs, WKRYs, and protein kinases. These results reveal promising alleles that control drought resistance, shedding light on the genetic basis of drought resistance and accelerating future efforts for drought resistance improvement in common bean.

Abstract Given the “carbon neutralization and carbon peak” policy, enhancing the low voltage ride-through (LVRT) capability of wind farms has become a current demand to ensure the safe and stable operation of power systems in the context of a possible severe threat of large-scale disconnection caused by wind farms. Currently, research on the LVRT of wind farms mainly focuses on suppressing rotor current and providing reactive current support, while the impact of active current output on LVRT performance has not been thoroughly discussed. This paper studies and reveals the relationship between the limit of reactive current output and the depth of voltage drop during LVRT for doubly-fed induction generator (DFIG) based wind farms. Specifically, the reactive current output limit of the grid-side converter is independent of the depth of voltage drop, and its limit is the maximum current allowed by the converter, while the reactive current output limit of the DFIG stator is a linear function of the depth of voltage drop. An optimized scheme for allocating reactive current among the STATCOM, DFIG stator, and grid-side converter is proposed. The scheme maximizes the output of active current while satisfying the standard requirements for reactive current output. Compared to traditional schemes, the proposed LVRT optimization strategy can output more active power during the LVRT period, effectively suppressing the rate of rotor speed increase, and improving the LVRT performance and fault recovery capability of wind farms. Simulation results verify the effectiveness of the proposed scheme.

Abstract The virtual synchronous generator (VSG) is a good solution for stabilizing the power system with high penetration of renewable energy. However, in case of serious unbalanced voltage disturbance/fault, the conventional VSG may lose voltage, inertia, and damping support characteristics to the grid and even can cause disconnection of renewable energy. This paper proposes an improved fault control strategy for VSG with the coordination of STATCOM. The proposed method can provide sufficient voltage support while keeping continuous system inertia and damping support under severe unbalanced fault. In the paper, an improved VSG and STATCOM control topology based on positive and negative sequence current control are first proposed so as to keep the damping and inertia support to grid during the grid fault. Secondly, the voltage support control method for the VSG with improved topology during unbalanced fault is introduced, which can achieve multiple control objectives, in terms of voltage support, current limitation, and active power output simultaneously. Then a coordination control scheme of improved VSG and STATCOM is developed so as to optimize the maximum control objectives in all possible scenarios, especially in the case of severe unbalanced fault. Finally, the effectiveness of the method is verified by using the MATLAB/SIMULINK simulation platform.

High-frequency electromagnetic fields refer to electromagnetic waves with frequencies ranging from 100 kHz to 300 MHz. High-frequency medium heating has the advantages of uniform heating, rapid energy consumption, and environmental protection. While it has a wide range of applications, the use of high-frequency dielectric heating in the bamboo industry is rare. Understanding the influence of bamboo temperature rise rate in high-frequency heating could promote bamboo industry development. In this work, curved bamboo was tested with high-temperature energy for continuous heating. The influence of moisture content of bamboo, sample thickness, and high-frequency processing power on temperature rise rate were studied. The results showed that the water content of bamboo affected the temperature rise rate. The effect of high-frequency heating was highest when the moisture content of bamboo was close to 11%. The thickness of sample had little effect on the temperature rise rate, but the high-frequency power had a significant effect on the temperature rise rate. The temperature rise rate of the lower and higher frequency power levels increased slowly and was close to constant. The heating power was 11 kW, and the temperature rise rate was the highest.

Shanghai University School of Medicine was a newly established medical college in 2018. It is founded on the national health development policies, international medical development trends and the close relationship between the advantages of new medical courses and medical artificial intelligence, it is dedicated to using intelligent medicine as the breakthrough point, training graduate students in two interdisciplinary medical engineering subjects as the priority, and implementing the integrated medical curriculum teaching reform. In this paper, we introduce the background of the integrated medical curriculum system at the Shanghai University School of Medicine, the horizontal and vertical integration of medical courses in interdisciplinary medical engineering subjects, the cross-integration of traditional integrated medical courses with other disciplines and specialties, and the transformation mode of medical science and technology innovation led by artificial intelligence under the support of three-dimensional curriculum integration, putting forward the prospect of the curriculum integration system and providing experiences and references for other medical schools. KEY MESSAGESThis paper introduces the necessity and feasibility of implementing integrated medical course teaching in a newly established medical college.This paper introduces the strategies and concrete measures we took to implement integrated medical course teaching.The analysis of examination papers and other evaluations revealed that the integrated medical teaching for graduate students with non-medical professional backgrounds is feasible.

Intestinal microbiota alterations may affect heart function through the production of gut-derived metabolites. Here the authors found that gut microbiota-derived TMAVA is a key determinant for the development of cardiac hypertrophy through inhibition of carnitine synthesis and subsequent fatty acid oxidation.

Abstract The present study was performed to explore whether and how impaired autophagy could modulate calcium/calmodulin-dependent protein kinase II (CAMKII)-regulated necrosis in the pathogenesis of acute pancreatitis (AP). Wistar rats and AR42J cells were used for AP modeling. When indicated, genetic regulation of CAMKII or ATG7 was performed prior to AP induction. AP-related necrotic injury was positively regulated by the incubation level of CAMKII. ATG7 positively modulated the level of CAMKII and necrosis following AP induction, indicating that there might be a connection between impaired autophagy and CAMKII-regulated necrosis in the pathogenesis of AP. microRNA (miR)-30b-5p was predicted and then verified as the upstream regulator of CAMKII mRNA in our setting of AP. Given that the level of miR-30b-5p was negatively correlated with the incubation levels of ATG7 after AP induction, a rescue experiment was performed and indicated that the miR-30b-5p mimic compromised ATG7 overexpression-induced upregulation of CAMKII-regulated necrosis after AP induction. In conclusion, our results indicate that ATG7-enhanced impaired autophagy exacerbates AP by promoting regulated necrosis via the miR-30b-5p/CAMKII pathway.

Abstract For the LCL‐type grid‐connected inverter, when the capacitor voltage feedforward is applied, the delay in the digital control system could change the phase characteristics of capacitor voltage feedback and affect the stability of the system. To solve the problem, a delay compensation method based on lead compensator is proposed in this paper, which can reduce the impacts of control delay on the capacitor voltage full feedforward control system. In this study, a lead compensator is introduced into the feedforward channel to compensate the phase lag, and the compensation function is designed to compensate for the phase margin but not change the system amplitude gain. The proposed strategy can keep the phase margin of the system above 25° when the grid impedance changes from 0 to 2.6 mH (0.1 p.u.), which enhances the robustness of the grid‐connected inverter under weak condition. The grid current distortion caused by grid background harmonics is greatly reduced and is kept within 4%. Moreover, the proposed strategy has fast dynamics while ensuring the system stability. Finally, the simulation and experimental results are presented to verify the effectiveness and robustness of the proposed control strategy.

Abstract In recent years, the modular multi‐level converter (MMC) has been widely used in high and medium voltage DC transmission systems because of its topological advantages. However, for an MMC with a two‐stage model predictive control (TSMPC) method, it is difficult to precisely and reasonably design the weighting factor in the cost function. Here, an improved TSMPC method is proposed which not only can avoid choosing the weighting factor for both first and second stage control but also can raise the output voltage level (OVL) to 2N + 1 without increasing computation burden. The discrete‐time mathematical model of the MMC is first derived. Two circulating current factors are introduced to calculate the optimal number of submodules (SMs) of the upper and lower arms in the first stage control. Secondly, the second stage control calculates the optimal number of SMs through the superior control and forms the optimisation array by adding or subtracting one SM. Then the objective function is developed, and the SMs with the minimum value of the objective function are selected for the final input. Finally, the algorithm of reducing switching frequency (RSF) is applied to achieve the balance of the SM capacitor voltage. The simulation and experimental results verify the effectiveness of the proposed method.

Objective: This study aimed to evaluate the quality of different non-waxy proso millet varieties. Methods: 21 non-waxy proso millet varieties were selected as experimental materials, and their nutritional composition, pasting characteristics, cooking characteristics and texture characteristics were measured. The quality difference and index correlations between different non-waxy proso millet varieties were analyzed and compared. Principal component analysis was used to evaluate their quality. Results: The texture characteristics, crude fat content, setback, breakdown and final viscosity had great differences among the tested varieties, and the variation coefficient of pasting temperature and starch content was small, which were relatively stable indexes. The results of principal component analysis showed that the first three principal components reflected 72.04% of the information of the original variable. The final viscosity, setback, hardness and gumminess were selected as the key indexes. The comprehensive quality of non-waxy proso millet varieties was evaluated according to the comprehensive score. Conclusion: Longmi 10, Neimi 9, Longmi 8, Yumi 3 and Ningmi 13 had the best comprehensive quality. The final viscosity, setback, hardness and gumminess could be used as representative indexes of quality of non-waxy proso millet to guide the selection of proso millet varieties.

To further improve air quality in China, especially ozone and fine particulate matter (PM2.5), additional reductions of volatile organic compounds (VOCs) are needed. Evaporative emissions from internal combustion engines (ICE) and hybrid vehicles are known to be an important source of VOCs in urban cities, which can be efficiently reduced through the introduction of more stringent regulatory standards. The most stringent global standards for evaporative emissions are the U.S. Tier 3 light-duty vehicle standards. This study compares the evaporative emissions of light-duty ICE vehicles designed to China 6 and U.S. Tier 3 regulations for both China and U.S. test conditions. Data are analyzed from manufacturer testing of full vehicle evaporative emissions conducted in the laboratory following U.S. Tier 3 and China 6 certification test methods utilizing Sealed Housing for Evaporative Determination (SHED) chambers equipped with Flame Ionization Detection (FID) analyzers. Vehicles designed to U.S. Tier 3 standards are observed to have 64% lower diurnal and hot soak evaporative emissions and 98% lower canister bleed emissions relative to vehicles designed to China 6 standards. U.S. Tier 3, U.S. Tier 2, and China 6 light-duty evaporative emission certification values submitted by manufacturers are also compared. The average of all current U.S. Tier 3 certifications is, on average, 52.4% lower than the average of all China 6 certifications. The results from the testing and data analysis of five vehicles, with comparison to the certification data, suggest that the introduction of standards equivalent in stringency to U.S. Tier 3 in China can significantly reduce evaporative emissions relative to China 6.

The article presents a novel strategy for enhancing the efficiency of machines that are used for complex structure machining. It proposes a low-cost five-axis four-linkage milling system as an alternative to the more expensive five-axis five-linkage system. Kinematic analysis of the machine tool is conducted to establish a correlation between the tool location data and the displacement of kinematic axes. An interpolation algorithm is then devised to determine a four-axis linkage milling strategy. The theoretical errors of the interpolation trajectory are observed to be reduced following the transformation. The research employs impeller processing as a case study, wherein the five-axis linkage machining path is translated into a more efficient five-axis four-linkage path using the interpolation algorithm. The practical application of this novel milling strategy confirms its effectiveness in processing the integral impeller within acceptable efficiency parameters. The results provide a theoretical foundation for the practical application of the low-cost five-axis four-linkage machining strategy in high-precision five-axis five-linkage machine tools.

Abstract Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibited the growth of P. purpureum and resulted in yellowing of the cells and thickening of the extracellular viscousness sheath. Under nitrogen stress, the contents of total lipids and exopolysaccharides in P. purpureum were increased by 65.2% and 188.0%, respectively. We demonstrate that the immediate response of P. purpureum to nitrogen deficiency is mediated by carbon flow to polysaccharide synthesis, while the synthesis of lipids is enhanced as a permanent energy storage substance at the later stage. Based on transcriptome annotation information, we elucidate the synthesis pathway of polysaccharides from P. purpureum from the perspective of glycosyl-donor interconversion, and demonstrate that the n-6 pathway is the main synthesis pathway of polyunsaturated fatty acids. This study not only provides a production strategy for polysaccharides and fatty acids by single-celled marine red algae P. purpureum, but also provides targets for further genetic modification.

Combining the core-shell structure with the optimization of surface composition and structure in the shell is a fantastic strategy to enhance the electrocatalytic performances. Here, we synthesized trimetallic Au@PtxSny core-shell nanoparticles (NPs) with tunable composition and structure of Pt-Sn alloyed shells. Impressively, the Au@PtSn core-shell NPs with hexagonal PtSn alloyed shells exhibited the highest mass activity and specific activity toward ethanol oxidation reaction (EOR) in alkaline electrolyte, which are 13.0 and 12.7 times higher than those of the commercial Pt/C. In addition, the Au@PtSn core-shell NPs displayed the best stability compared to commercial Pt/C, with only 44.8% loss vs. 86.8% loss in mass activity after 1,000 s due to the stronger anti-poisoning ability for reaction intermediates. The theory calculations reveal that the introduction of Au core and alloying Pt with Sn both endow Pt with an appropriate d-band center, and thus effectively boosting the EOR activity.

Acute pancreatitis (AP) is a common cause of a clinically acute abdomen. Crosstalk between acinar cells and leukocytes (especially macrophages) plays an important role in the development of AP. However, the mechanism mediating the interaction between acinar cells and macrophages is still unclear. This study was performed to explore the role of acinar cell extracellular vesicles (EVs) in the crosstalk between acinar cells and macrophages involved in the pathogenesis of AP. EVs derived from caerulein-treated acinar cells induced macrophage infiltration and aggravated pancreatitis in an AP rat model. Further research showed that acinar cell-derived EV miR-183-5p led to M1 macrophage polarization by downregulating forkhead box protein O1 (FoxO1), and a dual-luciferase reporter assay confirmed that FoxO1 was directly inhibited by miR-183-5p. In addition, acinar cell-derived EV miR-183-5p reduced macrophage phagocytosis. Acinar cell-derived EV miR-183-5p promoted the pancreatic infiltration of M1 macrophages and increased local and systemic damage in vivo. Subsequently, miR-183-5p overexpression in macrophages induced acinar cell damage and trypsin activation, thus further exacerbating the disease. In clinical samples, elevated miR-183-5p levels were detected in serum EVs and positively correlated with the severity of AP. EV miR-183-5p might play an important role in the development of AP by facilitating M1 macrophage polarization, providing a new insight into the diagnosis and targeted management of pancreatitis.Graphical abstract of the present study. In our caerulein-induced AP model, miR-183-5p was upregulated in injured acinar cells and transported by EVs to macrophages. miR-183-5p could induce M1 macrophage polarization through downregulation of FoxO1 and the release of inflammatory cytokines, which could aggravate AP-related injuries. Therefore, a vicious cycle might exist between injured ACs and M1 macrophage polarization, which is fulfilled by EV-transported miR-183-5p, leading to sustainable and progressive AP-related injuries.

Emerging evidence is examining the precise role of intestinal microbiota in the pathogenesis of type 2 diabetes. The aim of this study was to investigate the association of intestinal microbiota and microbiota-generated metabolites with glucose metabolism systematically in a large cross-sectional study in China. 1160 subjects were divided into three groups based on their glucose level: normal glucose group (n=504), prediabetes group (n=394), and diabetes group (n=262). Plasma concentrations of TMAO, choline, betaine, and carnitine were measured. Intestinal microbiota was measured in a subgroup of 161 controls, 144 prediabetes and 56 diabetes by using metagenomics sequencing. We identified that plasma choline [Per SD of log-transformed change: odds ratio 1.36 (95 confidence interval 1.16, 1.58)] was positively, while betaine [0.77 (0.66, 0.89)] was negatively associated with diabetes, independently of TMAO. Individuals with diabetes could be accurately distinguished from controls by integrating data on choline, and certain microbiota species, as well as traditional risk factors (AUC=0.971). KOs associated with the carbohydrate metabolism pathway were enhanced in individuals with high choline level. The functional shift in the carbohydrate metabolism pathway in high choline group was driven by species Ruminococcus lactaris, Coprococcus catus and Prevotella copri. We demonstrated the potential ability for classifying diabetic population by choline and specific species, and provided a novel insight of choline metabolism linking the microbiota to impaired glucose metabolism and diabetes.

Abstract The optimised droop control method is proposed to achieve the state‐of‐charge (SoC) balance among parallel‐connected distributed energy storage units in islanded DC microgrid, which considers the difference of line impedance, initial state‐of‐charge values and capacities among distributed energy storage units. Since the droop control is the basic control strategy for load sharing in DC microgrid applications, however, the load sharing accuracy is degraded under conventional droop control method due to the unmatched line impedance in reality. Meanwhile, the initial state‐of‐charge values and capacities of each distributed energy storage unit are usually different. Hence, the state of charge for distributed energy storage units cannot be balanced. In order to prolong the lifetime of the distributed energy storage units and avoid the overuse of a certain distributed energy storage unit, the optimised droop control strategy based on sample and holder is designed, by modifying the droop coefficient adaptively, the accurate load sharing and balanced state of charge among distributed energy storage units are both obtained. Finally, the performance of the proposed control scheme is accessed through a series cases on technologies real‐time digital simulator (RTDS) and its effectiveness is verified.

The depletion and environmental pollution associated with traditional fossil energy sources has generated great interest in the development of new energy. Among the kinds of new-energy batteries, lithium-ion batteries have the advantages of small size, high energy density, a long life cycle, zero emissions, and no pollution. These batteries are widely used in many industries and fields, including vehicles. Currently, assessments of the health status of lithium-ion batteries have become a hot research topic. The lithium-ion battery has complex electrochemical characteristics and its capacity tends to degrade with cyclic charges and discharges. When its capacity degrades to the failure threshold (usually 70%–80% of rated capacity), the life of lithium ion battery is considered to have reached an end. Therefore, investigations to better predict the remaining useful life of a lithium-ion battery can help to improve system reliability and prevent accidents. Battery-system health evaluations have important research and application value. In this study, the voltage change curves of the lithium-ion battery were investigated with discharge time under equivalent cycle conditions and different cycle times. By analyzing the slope change rule of the derivative function at an equivalent characteristic point for different cycle times, the life degradation curves of the lithium-ion battery under equivalent cycle conditions were established. Using the NASA and self-test JZ equivalent cycle batteries, the intersection point of the specific-slope straight line and curve at early and late stages of discharge was taken as the equivalent feature points for predicting the equivalent cycle life. Based on these two groups of feature points, Mini and Mlat degradation models were established, respectively. To verify this method, other batteries in the equivalent-cycle battery pack were tested. The results of the test data set validate the prediction accuracy and stability, which has strong application value.

Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging in vivo, a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration in vivo, electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring in vivo added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind.

Mitotic spindle function depends on the precise regulation of microtubule dynamics and microtubule sliding. Throughout mitosis, both processes have to be orchestrated to establish and maintain spindle stability. We show that during anaphase B spindle elongation in Schizosaccharomyces pombe, the sliding motor Klp9 (kinesin-6) also promotes microtubule growth in vivo. In vitro, Klp9 can enhance and dampen microtubule growth, depending on the tubulin concentration. This indicates that the motor is able to promote and block tubulin subunit incorporation into the microtubule lattice in order to set a well-defined microtubule growth velocity. Moreover, Klp9 recruitment to spindle microtubules is dependent on its dephosphorylation mediated by XMAP215/Dis1, a microtubule polymerase, creating a link between the regulation of spindle length and spindle elongation velocity. Collectively, we unravel the mechanism of anaphase B, from Klp9 recruitment to the motors dual-function in regulating microtubule sliding and microtubule growth, allowing an inherent coordination of both processes.

Abstract Currently, preliminary results have confirmed the existence of receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis of pancreatic acinar cells during early acute pancreatitis (AP), which might be a potential target for the effective regulation of necroinflammatory injury. However, the exact effect of receptor-interacting protein kinase 1 (RIPK1)-dependent regulated acinar cell necrosis on AP is still uncertain. In our study, we first explored the changes in the degree of local and systemic inflammation in AP rats when the activation of acinar cell RIPK1 was inhibited. The RIPK1 inhibitor Nec-1 was used to treat rats, and the levels of related inflammatory markers, necrosis indicators and apoptotic indicators were measured. Changes in pancreatic nuclear factor κB (NF-κB) and aquaporin 8 (AQP8) expression were noted. Next, the expression of AQP8 in AR42J cells was inhibited, and the degree of cell necrosis and inflammatory damage was found to be significantly reduced. Most importantly, we demonstrated that the RIPK1/NF-ĸB/AQP8 axis might be a potential regulatory pathway mediating RIPK1-dependent regulated acinar cell necrosis in early AP. Finally, we used the NF-κB inhibitor PDTC and Nec-1 to treat rats in different groups and measured the degree of pathological pancreatic injury, the activation of RIPK1, and the expression of NF-κB and AQP8. In summary, we hypothesized that there might be a RIPK1/NF-ĸB/AQP8 pathway controlling RIPK1-dependent regulated necrosis of acinar cells in AP, which might be a promising therapeutic target against AP-related injury.

Abstract Background Ubiquitin-like protein 4A (UBL4A) plays a significant role in protein metabolism and the maintenance of cellular homeostasis. In cancer, UBL4A represses tumorigenesis and is involved in various signaling pathways. Pancreatic ductal adenocarcinoma (PDAC) is still a major cause of cancer-related death and the underlying molecular mechanism of UBL4A and PDAC remains unknown. Methods First, the prognostic role of UBL4A and its expression in human PDAC patients and in pancreatic cancer cell lines were detected by survival analysis and qRT-PCR, western blotting, and immunohistochemistry. Next, the effects of UBL4A on proliferation and metastasis in pancreatic cancer were evaluated by functional assays in vitro and in vivo. In addition, chloroquine was introduced to determine the role of autophagy in UBL4A-related tumor proliferation and metastasis. Ultimately, coimmunoprecipitation was used to confirm the interaction between UBL4A and lysosome associated membrane protein-1 (LAMP1), and western blotting was performed to explore the UBL4A mechanism. Results We found that UBL4A was decreased in PDAC and that high levels of UBL4A correlated with a favorable prognosis. We observed that UBL4A inhibited tumor proliferation and metastasis through suppression of autophagy, a critical intracellular catabolic process that reportedly protects cells from nutrient starvation and other stress conditions. UBL4A caused impaired autophagic degradation in vitro, a crucial process in autophagy, by disturbing the function of lysosomes and contributing to autophagosome accumulation. We found a positive correlation between UBL4A and LAMP1. Furthermore, UBL4A caused lysosomal dysfunction by directly interacting with LAMP1, and LAMP1 overexpression reversed the antitumor effects of UBL4A in pancreatic cancer. In addition, we demonstrated that UBL4A suppressed tumor growth and metastasis in a pancreatic orthotopic tumor model. Conclusions These findings suggest that UBL4A exerts an antitumor effect on autophagy-related proliferation and metastasis in PDAC by directly targeting LAMP1. Herein, we describe a novel mechanism of UBL4A that suppresses the progression of pancreatic cancer. UBL4A might be a promising target for the treatment and prognostication of PDAC.

The aim of the study was investigate the impact of body-mass factors (BMF) on setup displacement during pelvic radiotherapy in patients with lower abdominal cancers.

Abstract Radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) often leads to aggressive local recurrence and increased metastasis, and vascular integrity and platelets are implicated in tumor metastasis. However, whether interactions between endothelial cells and platelets induce endothelial permeability in HCC after insufficient RFA remains unclear. Here, significantly increased CD62P‐positive platelets and sP‐selectin in plasma are observed in HCC patients after RFA, and tumor‐associated endothelial cells (TAECs) activate platelets and are susceptible to permeability after heat treatment in the presence of platelets in vitro. In addition, tumors exhibit enhanced vascular permeability after insufficient RFA in mice; heat treatment promotes platelets‐induced endothelial permeability through vascular endothelial (VE)‐cadherin, and ICAM‐1 upregulation in TAECs after heat treatment results in platelet activation and increased endothelial permeability in vitro. Moreover, the binding interaction between upregulated ICAM‐1 and Ezrin downregulates VE‐cadherin expression. Furthermore, platelet depletion or ICAM‐1 inhibition suppresses tumor growth and metastasis after insufficient RFA in an orthotopic tumor mouse model, and vascular permeability decreases in ICAM‐1−/− mouse tumor after insufficient RFA. The findings suggest that ICAM‐1 activates platelets and promotes endothelial permeability in TAECs through VE‐cadherin after insufficient RFA, and anti‐platelet and anti‐ICAM‐1 therapy can be used to prevent progression of HCC after insufficient RFA.

Disruption of endothelial monolayer integrity is the primary instigating factor for many cardiovascular diseases. High density lipoprotein (HDL) oxidized by heme enzyme myeloperoxidase (MPO) is dysfunctional in promoting endothelial repair. Apolipoprotein A-1 mimetic 4F with its pleiotropic benefits has been proven effective in many in vivo models. In this study we investigated whether 4F promotes endothelial repair and restores the impaired function of oxidized HDL (Cl/NO2-HDL) in promoting re-endothelialization. We demonstrate that 4F and Cl/NO2-HDL act on scavenger receptor type I (SR-B1) using human aorta endothelial cells (HAEC) and SR-B1 (-/-) mouse aortic endothelial cells. Wound healing, transwell migration, lamellipodia formation and single cell migration assay experiments show that 4F treatment is associated with a recovery of endothelial cell migration and associated with significantly increased endothelial nitric oxide synthase (eNOS) activity, Akt phosphorylation and SR-B1 expression. 4F increases NO generation and diminishes oxidative stress. In vivo, 4F can stimulate cell proliferation and re-endothelialization in the carotid artery after treatment with Cl/NO2-HDL in a carotid artery electric injury model but fails to do so in SR-B1(-/-) mice. These findings demonstrate that 4F promotes endothelial cell migration and has a potential therapeutic benefit against early endothelial injury in cardiovascular diseases.

Hybrid reactive power compensation (HRPC) which consists of series compensation and a stepped-controlled shunt reactor will see use in China's future ultra-high-voltage (UHV) transmission system. As a result, some new characteristics of the secondary arc during a single-phase-to-ground fault on UHV transmission lines with HRPC may be raised. In this paper, the secondary arc characteristics during the single-phase auto-reclosure (SPAR) operation have been simulated with regard to the UHV AC system installed with HRPC. Based on the UHV system model with HRPC and its secondary arc model, the influence mechanism of HRPC on secondary arc current is theoretically derived with the simulated waveforms and the Fourier analysis. Besides, the relationship among the secondary arc current, the arcing time with different HRPC compensation degrees, neutral reactance, as well as the grounding fault locations have been investigated in detail. Finally, an improved time sequential control strategy of bypass breaker and SPAR has been put forward. The research presented in this paper will provide necessary theoretical basis and technical support for the future application of HRPC in UHV transmission lines.

Stereotactic ablative radiotherapy (SABR) is a promising option for non-operated early-stage non-small cell lung cancer (NSCLC) compared to conventional fractionated radiotherapy (CFRT). However, results from conclusive randomized controlled trials are not yet available. The aim of our study was to explore the effectiveness of SABR vs. CFRT for non-operated early-stage NSCLC.

Corona inception voltage is of great significance for the design of ultra‐high‐voltage direct current transmission lines. In this study, based on the change rule of frequency‐domain characteristics of corona current, a new method for calculating corona inception voltage is proposed. First, corona current waveforms are collected through experiments and the range of measuring voltages is determined according to the frequency spectrum characteristics of corona current. Then, the curves representing the relationship between the change rate of corona current octave power and the voltage at different centre frequencies (6–20 kHz) are obtained through least‐square fitting. The voltage at which the fitted curve reaches the first peak value with the rise of voltage is identified as corona inception voltage. Finally, with the proposed method, the corona inception voltage for a six bundled conductor system in the biggest outdoor corona cage of the world is obtained. Combined with the experimental results of A‐weighted sound level of audible noise, the comparisons between the obtained corona inception voltage and that calculated with I/U−U curve fitting method, I−U curve fitting method and an audible noise‐based method prove that the proposed method is reasonable.

Microtubules in the mitotic spindle are organised by microtubule-associated proteins. In the late stage of mitosis, spindle microtubules are robustly organised through bundling by the antiparallel microtubule bundler Ase1/PRC1. In early mitosis, however, it is not well characterised as to whether spindle microtubules are actively bundled, as Ase1 does not particularly localise to the spindle at that stage. Here we show that the conserved microtubule-associated protein CLASP (fission yeast Peg1/Cls1) facilitates bundling of spindle microtubules in early mitosis. The peg1 mutant displayed a fragile spindle with unbundled microtubules, which eventually resulted in collapse of the metaphase spindle and abnormal segregation of chromosomes. Peg1 is known to be recruited to the spindle by Ase1 to stabilise antiparallel microtubules in late mitosis. However, we demonstrate that the function of Peg1 in early mitosis does not rely on Ase1. The unbundled spindle phenotype of the peg1 mutant was not seen in the ase1 mutant, and Peg1 preferentially localised to the spindle even in early mitosis unlike Ase1. Moreover, artificial overexpression of Ase1 in the peg1 mutant partially suppressed unbundled microtubules. We thus conclude that Peg1 bundles microtubules in early mitosis, in a distinct manner from its conventional Ase1-dependent functions in other cell cycle stages.

Labeling of Pamidronate (PAM) with 99mTc was studied by a direct labeling method in the presence of SnCl2•2H2O as reducing agent. The influences of the concentration of SnCl2•2H2O, PAM concentration and pH value, reaction time on labeling yield were investigated. The optimum labeling was determined. The results showed that the radiochemistry purity of 99mTc-PAM was more than 95%. Biodistribution studies in normal mice and rats showed very high uptake of 99mTc-PAM and long retain in bone. 99mTc-PAM was washed out from the blood very quickly. In addition, considerable uptake in the kidneys indicated this complex was excreted mainly by renal pathway. On the other hand, the radioactivity in liver, lung and heart was negligible. It could be known from SPECT images that 99mTc-PAM would be an excellent bone-imaging agent. Bone uptake of 99mTc-PAM was higher than that of 99mTc-MDP in mice. This study suggested that 99mTc-PAM was a promising bone imaging agent and further study was worthwhile.

This paper presents an improved inverse-time over-current protection method based on the compound fault acceleration factor and the beetle antennae search (BAS) optimization method for a microgrid. The proposed method can not only significantly increase the operation speed of the inverse-time over-current protection but also improve the protection coordination by considering the possible influential factors in terms of microgrid operation modes, distributed generation (DG) integration status, fault types, and positions, which are marked as the most challenging problems for over-current protection of a microgrid. In this paper, a new Time Dial Setting (TDS) of inverse-time protection is developed by applying a compound fault acceleration factor, which can notably accelerate the speed of protection by using low-voltage and short-circuit impedance during the fault. In order to improve the protection coordination, the BAS algorithm is then used to optimize the protection parameters of the pick-up current, TDS, and the inverse time curve shape coefficient. Finally, case studies and various evaluations based on DIgSILENT/Power Factory are carried out to illustrate the effectiveness of the proposed method.

The microtubule cytoskeleton plays important roles in cell polarity, motility and division. Microtubules inherently undergo dynamic instability, stochastically switching between phases of growth and shrinkage. In cells, some microtubule-associated proteins (MAPs) and molecular motors can further modulate microtubule dynamics. We present here the fission yeast mtr1+, a new regulator of microtubule dynamics that appears to be not a MAP or a motor. mtr1-deletion (mtr1Δ) primarily results in longer microtubule dwell-time at the cell tip cortex, suggesting that mtr1p acts directly or indirectly as a destabilizer of microtubules. mtr1p is antagonistic to mal3p, the ortholog of mammalian EB1, which stabilizes microtubules. mal3Δ results in short microtubules, but can be partially rescued by mtr1Δ, as the double mutant mal3Δ mtr1Δ exhibits longer microtubules than mal3Δ single mutant. By sequence homology, mtr1p is predicted to be a component of the ribosomal quality control complex. Intriguingly, deletion of a predicted ribosomal gene, rps1801, also resulted in longer microtubule dwell-time similar to mtr1Δ. The double-mutant mal3Δ rps1801Δ also exhibits longer microtubules than mal3Δ single mutant alone. Our study suggests a possible involvement of mtr1p and the ribosome complex in modulating microtubule dynamics.

In this paper, carbon nanotubes (CNTs) were added to activated carbon to enhance the mesopores. At first, different amounts of CNTs were added to phenolic resin and composite spheres were synthesized by suspension polymerization. Then, by carbonizing these spheres at 600°C followed by steam activation at 850°C for more than 90 min, we prepared highly developed porous CNTs/activated carbon composite spheres. The composite spheres were characterized by a laser particle size analyzer, scanning electron microscopy, Raman spectrum, and nitrogen adsorption-desorption isotherms. Results showed that the composite spheres had good sphericity even with high proportion of CNTs (45 wt.%). Analysis also showed that the pore size distribution of the composite spheres containing CNTs was “multi-peak”, especially with 20–100-nm pores. The improved 20–100-nm porous structures in the composite spheres can be ascribed to aggregated pores of CNT bundles. The amount of vitamin B12 adsorbed on the 45 wt.% CNT composite spheres was as high as 32.38 mg/g. The spheres could be used as adsorbents for middle-molecular-weight toxins or large molecules in hemoperfusion.

Catalytic doping plays an important role in enhancing the hydrogen storage performance of MgH2, while finding an efficient and reversible catalyst remains to be a great challenge in enhancing the de/rehydrogenation properties of MgH2. Herein, a bidirectional nano-TiH1.971 catalyst was prepared by a wet chemical ball milling method and its effect on hydrogen storage properties of MgH2 was studied. The results showed that all the TiH1.971 nanoparticles were effective in improving the de/rehydrogenation kinetics of MgH2. The MgH2 composites doped with TiH1.971 could desorb 6.5 wt % H2 in 8 min at 300 °C, while the pure MgH2 only released 0.3 wt % H2 in 8 min and 1.5 wt % H2 even in 50 min. It was found that the smaller the size of the TiH1.971 particles, the better was the catalytic effect in promoting the performance of MgH2. Besides, the catalyst concentration also played an important role and the 5 wt %-c-TiH1.971 modified system was found to have the best hydrogen storage performance. Interestingly, a significant hydrogen absorption amount of 4.60 wt % H2 was evidenced for the 5 wt %-c-TiH1.971 doped MgH2 within 10 min at 125 °C, while MgH2 absorbed only 4.11 wt% hydrogen within the same time at 250 °C. The XRD results demonstrated that the TiH1.971 remained stable in cycling and could serve as an active site for hydrogen transportation, which contributed to the significant improvement of the hydrogen storage properties of MgH2.

Along with social economy development, the total water consumption increased year by year. The conflicts between water supply and water consumption is growing. Water resources optimization dispatch, which is used to allocate water resources to meet the demand of water user on both time scale and spatial scale, plays an important role in water resources management. Aiming at improving the spatial allocation ability of traditional optimization algorithms, a uniform spatial allocation strategy is proposed accordingly. The proposed method is used to improve the performance of optimization algorithm to obtain solutions which can uniform the water supply in spatial scale. Simulation results show that the maximum ratio of water deficiency gained by the proposed method is smaller than that obtained by the original algorithm. The proposed method is effective to balance the demand of water users in spatial scale. Copyright © 2018 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the 10th International Conference on Applied Energy (ICAE2018).