54 results on '"Lian-Di Zhou"'
Search Results
2. Specific capture and determination of glycoprotein using a hybrid epitopes and monomers-mediated molecular-imprinted polymer enzyme-free electrochemical biosensor
- Author
-
Jia-Yuan He, Hui-Xian Xu, Qin Li, Qin-Yue Zheng, Chong-Zhi Wang, Lian-Di Zhou, Ling Luo, Qi-Hui Zhang, and Chun-Su Yuan
- Subjects
Analytical Chemistry - Published
- 2023
3. Constructing a dummy-template adsorbent using magnetic molecular-imprinted chitosan for rapid enrichment of flavonoids compounds from Penthorum chinense Pursh
- Author
-
Li-Li Yang, Ting Li, Dan-Dan Yang, Yan-Jun Li, Jia-Yuan He, Lian-Di Zhou, Qi-Hui Zhang, Chong-Zhi Wang, and Chun-Su Yuan
- Subjects
Spectroscopy ,Analytical Chemistry - Published
- 2023
4. An edible molecularly imprinted material prepared by a new environmentally friendly deep eutectic solvent for removing oxalic acid from vegetables and human blood
- Author
-
Yan-Jun Li, Jia-Yuan He, Qing-Yao Li, Li-Li Yang, Rong-Rong Ma, Chong-Zhi Wang, Lian-Di Zhou, Qi-Hui Zhang, and Chun-Su Yuan
- Subjects
Molecular Imprinting ,Oxalic Acid ,Vegetables ,Deep Eutectic Solvents ,Solvents ,Humans ,Adsorption ,Biochemistry ,Analytical Chemistry - Abstract
A novel deep eutectic solvent-magnetic molecularly imprinted polymer (DES-MMIP) for the specific removal of oxalic acid (OA) was prepared by an environmentally friendly deep eutectic solvent, consisting of betaine, citric acid, and glycerol, which acted as the functional monomer for polymerization. The structure and morphology of DES-MMIPs were studied by X-ray diffraction, scanning and transmission electron microscopy, thermal gravimetric analysis, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. DES-MMIPs had a core-shell structure, with magnetic iron oxide as the core, and showed good thermal stability and high adsorption capacity (18.73 mg/g) for OA. The adsorption process of OA by DES-MMIPs followed the pseudo-second-order kinetic model and Langmuir isotherm model. DES-MMIPs had significant selectivity for OA and their imprinting factor was 3.26. When applied to real samples, high performance liquid chromatography analysis showed that DES-MMIPs could remove OA from both spinach and blood serum. These findings provide potential methods for removal of OA from vegetables and for specific removal of OA in renal dialysis.
- Published
- 2022
5. Swelling of Multilayered Calcium Alginate Microspheres for Drug-Loaded Dressing Induced Rapid Lidocaine Release for Better Pain Control
- Author
-
Rong-Rong, Ma, Hui-Xian, Xu, Li, Ni, Jia-Min, Xiong, Yi-Lin, Chen, Jia-Yuan, He, Qin, Li, Li-Li, Yang, Lian-Di, Zhou, Qi-Hui, Zhang, and Ling, Luo
- Subjects
Chitosan ,Complementary and alternative medicine ,Alginates ,Humans ,Lidocaine ,Gelatin ,Pain ,General Medicine ,Bandages ,Microspheres - Abstract
The development of effective drug-loaded dressings has been considered a hot research topic for biomedical therapeutics, including the use of botanical compounds. For wound healing, adequate dressings can provide a good microenvironment for drug release, such as lidocaine. Biological macromolecular materials such as alginate show excellent properties in wound management. This study involves the preparation and evaluation of biocompatible multilayered-structure microspheres composed of chitosan, porous gelatin, and calcium alginate microspheres. The multilayered structure microspheres were named chitosan@ porous gelatin@ calcium alginate microspheres (CPAMs) and the drugs were rapidly released by the volume expansion of the calcium alginate microspheres. The in vitro release curve revealed that the peak release of lidocaine from CPAMs was reached within 18[Formula: see text]min. After 21[Formula: see text]min, the remaining lidocaine was then slowly released, and the active drug release was converted to a passive drug release phase. The initial release effect of lidocaine was much better than that reported in the published studies. Additionally, blood coagulation experiments showed that CPAMs coagulated blood in 60[Formula: see text]s, and the blood liquidity of the CPAMs group was worse than that of the woundplast group. Therefore, the coagulation characteristics of CPAMs were superior to the commonly used woundplast containing lidocaine healing gel. These study outcomes indicated that the CPAMs acted as fast-release dressings for faster pain control and better coagulation properties.
- Published
- 2022
6. Recognition and analysis of biomarkers in tumor microenvironments based on promising molecular imprinting strategies with high selectivity
- Author
-
Jia-Yuan He, Qin Li, Hui-Xian Xu, Qin-Yue Zheng, Qi-Hui Zhang, Lian-Di Zhou, Chong-Zhi Wang, and Chun-Su Yuan
- Subjects
Spectroscopy ,Analytical Chemistry - Published
- 2023
7. Constructing electrochemical sensor using molecular-imprinted polysaccharide for rapid identification and determination of L-tryptophan in diet
- Author
-
Yan-Jun Li, Li-Li Yang, Li Ni, Jia-Min Xiong, Jia-Yuan He, Lian-Di Zhou, Ling Luo, Qi-Hui Zhang, and Chun-Su Yuan
- Subjects
General Medicine ,Food Science ,Analytical Chemistry - Published
- 2023
8. Fast exhaustive enrichment and electrochemical quantitative detection of anthocyanins from natural products by using dual responsive and dummy molecularly imprinted polymers
- Author
-
Zhuang-Fei Jiang, Qin Li, Qing-Yao Li, Hui-Xian Xu, Jia-Yuan He, Chong-Zhi Wang, Lian-Di Zhou, Qi-Hui Zhang, Ling Luo, and Chun-Su Yuan
- Subjects
Spectroscopy ,Analytical Chemistry - Published
- 2022
9. Synergistic recognition of transferrin by using performance dual epitope imprinted polymers
- Author
-
Chong-Zhi Wang, Li-Li Yang, Qi-Hui Zhang, Zhining Xia, Qing-Yao Li, Jia-Yuan He, Lian-Di Zhou, Chun-Su Yuan, and Rong-Rong Ma
- Subjects
chemistry.chemical_classification ,Glycan ,biology ,Chemistry ,Polymers ,Molecularly imprinted polymer ,Transferrin ,Biochemistry ,High-performance liquid chromatography ,Combinatorial chemistry ,Epitope ,Analytical Chemistry ,Molecular Imprinting ,chemistry.chemical_compound ,Epitopes ,Adsorption ,Targeted drug delivery ,Acrylamide ,biology.protein ,Environmental Chemistry ,Humans ,Spectroscopy - Abstract
Transferrin (Trf) is a new type of active drug targeting carrier and disease biomarker that regulates the balance of iron ions in human body. The recognition and isolation of Trf is of great significance for disease diagnosis and treatment. Thus, a new type of magnetic dual affinity epitope molecularly imprinted polymer coated on Fe3O4 nanoparticles (Fe3O4@DEMIP) was successfully prepared for specific recognition of Trf. C-terminal nonapeptide and Trf glycan were selected as bi-epitope templates for metal chelation and boron affinity immobilization, respectively. 4-vinylphenylboric acid (4-VP), N-isopropyl acrylamide (NIPAM) and zinc acrylic were used as functional monomers. Results showed that Fe3O4@DEMIP exhibited excellent specific recognition ability adsorption capacity toward Trf, with an adsorption of 43.96 mg g−1 (RSD = 3.28%) and a more satisfactory imprinting factor (about 6.60) than that of other reported imprinting methods. In addition, Fe3O4@DEMIP displayed pH, temperature and magnetic sensitivity properties to realize temperature and pH-controlled recognition and release of target proteins and magnetic rapid separation. Furthermore, the Fe3O4@DEMIP coupled with high-performance liquid chromatography (HPLC) analysis was successfully used for specific recognition of Trf in biosamples. This study provides a reliable protocol for preparing metal chelation and boron affinity dual affinity bi-epitope molecularly imprinted polymers for synergistic and efficient recognition of biomacromolecules in the complex biological systems.
- Published
- 2021
10. Debittering of lemon juice using surface molecularly imprinted polymers and the utilization of limonin
- Author
-
Guocan Zheng, Chong-Zhi Wang, Yu-Zhen Zhang, Zhining Xia, Lian-Di Zhou, Jia-Wei Zhang, Qi-Hui Zhang, Chun-Su Yuan, and Ling Tan
- Subjects
Limonins ,Citrus ,Sorbent ,Limonin ,Clinical Biochemistry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,Molecular Imprinting ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adsorption ,Animals ,chemistry.chemical_classification ,Wound Healing ,Chromatography ,010401 analytical chemistry ,Molecularly imprinted polymer ,Cell Biology ,General Medicine ,Polymer ,0104 chemical sciences ,Fruit and Vegetable Juices ,chemistry ,Polymerization ,Selectivity - Abstract
In this work, surface molecularly imprinted polymers (SMIPs) were prepared as a specific sorbent to remove the limonin from the lemon juice for the first time, and then the MIPs containing limonin were directly made into a water-soluble gel to treat inflammation of mice. The resulting polymers were characterized by scanning electron microscopy, transmission electron microscopy and Fourier transform infrared spectrometer spectra. And the polymerization conditions and adsorption performances of the resultant nanomaterials were further investigated in detail. Results showed that the MIPs have higher adsorption capacity (27.72 mg/g) compared with surface molecularly non-imprinted polymers (NIPs) (8.12 mg/g). The selectivity experiment indicated that the polymers had excellent selective recognition for limonin and the selectivity factors were calculated as 2.75 and 1.83 for nomilin and obakunone, respectively. The MIPs were successfully used as adsorbent for selectively removing limonin from lemon juice and the MIPs extracted almost all the limonin from lemon juice according to the HPLC results. Furthermore, the MIPs with limonin were processed into water-soluble gel, which can be used to reduce the inflammation and enhance wound healing of model mice.
- Published
- 2019
11. Selective separation and inexpensive purification of paclitaxel based on molecularly imprinted polymers modified with ternary deep eutectic solvents
- Author
-
Zhuang-Fei Jiang, Jia-Yuan He, Zhining Xia, Chun-Su Yuan, Rong-Rong Ma, Ling Tan, Qi-Hui Zhang, Chong-Zhi Wang, and Lian-Di Zhou
- Subjects
Thermogravimetric analysis ,Paclitaxel ,Clinical Biochemistry ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,Molecular Imprinting ,Adsorption ,Molecularly Imprinted Polymers ,Desorption ,Specific surface area ,Drug Discovery ,Spectroscopy, Fourier Transform Infrared ,Fourier transform infrared spectroscopy ,Spectroscopy ,chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,010401 analytical chemistry ,Solid Phase Extraction ,Molecularly imprinted polymer ,Polymer ,0104 chemical sciences ,Chemical engineering ,Selective adsorption ,Solvents - Abstract
Paclitaxel (PTX) is a powerful anticancer natural product, with its separation and purification having been widely studied. In this work, new molecular imprinted polymers (MIPs) using deep eutectic solvents (DESs) with different molar ratios were prepared as functional monomers. These were then used as adsorbents in solid phase extraction (SPE) for the separation of PTX from its structural analogs. The polymers were characterized by energy disperive X-rays (EDX), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and fourier transform infrared spectroscopy (FT-IR). The results suggested that the formative regular DES-MIPs had an even pore-size distribution and a large specific surface area. The dynamic adsorption and static adsorption showed that the DES-MIPs had excellent adsorption performance, with a maximum adsorption capacity and optimum adsorption time of 87.08 mg/g and 180 min, respectively. The selective adsorption experiments showed that the material had outstanding selectivity, and the maximum selectivity factor was 6.20. For stability, after six consecutive adsorption and desorption cycles, the DES-MIPs maintained the perfect stability and reusability. Furthermore, the fabricated SPE column was successfully utilized for extracting and eluting PTX. This study provides a reliable protocol for the separation and purification PTX from its structural analogs and the DES-MIPs materials have excellent potential application value in pharmaceutical industry.
- Published
- 2020
12. [Rotective Effects of Quercetin Against the Triptolide Induced Liver Injury and Relevant Mechanism Study]
- Author
-
Cai-Bing, Wei, Lian-di, Zhou, Jia-Wei, Zhang, Qi-Hui, Zhang, and Kun, Tao
- Subjects
Mice, Inbred C57BL ,Mice ,Oxidative Stress ,Random Allocation ,Liver ,Animals ,Cytokines ,Epoxy Compounds ,Quercetin ,Chemical and Drug Induced Liver Injury ,Diterpenes ,Phenanthrenes ,Antioxidants - Abstract
To explore the protective effects of quercetin (QE) on triptolide (TP) induced liver injury and the relevant mechanism.Forty C57BL/6 mice were equally divided into 4 groups, control group, TP model group, 20 mg/kg QE treatment group and 80 mg/kg QE treatment group randomly. The 20 mg/kg and 80 mg/kg QE groups were gastrointestinal administration with QE at the dose of 0.2 mL/10 g for 10 d, twice daily, while other groups were administrated with equivalent normal saline. Four hours post the last dose, animals were gastrointestinal administered with TP at a dose of 500 μg/kg per mouse, except for NS control. All the mice were sacrificed 22 h later, blood and liver tissue samples were collected. The pathologic change of liver tissue was detected by HE staining. The level of aminotransferase (AST) and aspartate alanine aminotransferase (ALT) in serum, and the level of glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue homogenates were detected using the commercial kits. The level of interleukin (IL)-17, IL-10 and IL-6 in liver tissue homogenates was measured by ELISA. Hepatic expression of Toll-like receptor 4 (TLR4) was detected by Western blot.Compared with the control group, in the TP model group, hepatic lobule structure atrophied and even disappeared, hepatic cell necrosis and inflammatory cell infiltration are obvious. Additionally, in TP model group, serum ALT, AST and MDA levels were significantly increased, SOD and GSH levels were decreased, IL-6 and IL-17 levels were increased, IL-10 levels were decreased, and TLR4 protein levels were increased (Quercetin can reduce TP-induced liver injury by reducing oxidative damage, promoting antioxidant and regulating cytokine secretion.
- Published
- 2019
13. Preparation and evaluation of temperature and magnetic dual-responsive molecularly imprinted polymers for the specific enrichment of formononetin
- Author
-
Zhining Xia, Chun-Su Yuan, Chong-Zhi Wang, Qi-Hui Zhang, Ling Tan, Lian-Di Zhou, Jia-Wei Zhang, and Yu-Zhen Zhang
- Subjects
Polymers ,Surface Properties ,Filtration and Separation ,02 engineering and technology ,01 natural sciences ,Polymerization ,Analytical Chemistry ,Molecular Imprinting ,chemistry.chemical_compound ,Adsorption ,Formononetin ,chemistry.chemical_classification ,Magnetic Phenomena ,010401 analytical chemistry ,Temperature ,Molecularly imprinted polymer ,Polymer ,021001 nanoscience & nanotechnology ,Isoflavones ,0104 chemical sciences ,chemistry ,Methacrylic acid ,Selective adsorption ,Trifolium ,0210 nano-technology ,Molecular imprinting ,Nuclear chemistry - Abstract
Thermo-responsive magnetic molecularly imprinted polymers were prepared by simple surface molecular imprinting polymerization for the selective adsorption and enrichment of formononetin from Trifolium pretense by temperature regulation. Using formononetin as a template, N-isopropylacrylamide as the thermo-responsive functional monomer, and methacrylic acid as an assisting functional monomer, the polymers were synthesized on the surface of the magnetic substrate. The results show that imprinted polymers attained controlled adsorption of formononetin in response to the temperature change, with large adsorption capacity (16.43 mg/g), fast kinetics (60 min) and good selectivity at 35°C compared with that at 25 and 45°C. The selectivity experiment indicated that the materials had excellent recognition ability for formononetin and the selectivity factors were between 1.32 and 2.98 towards genistein and daidzein. The excellent linearity was attained in the range of 5-100 μg/mL, with low detection limits and low quantitation limits of 0.017 and 0.063 μg/mL, respectively. Furthermore, the thermo-responsive magnetic molecularly imprinted polymers were successfully utilized for enriching and purifying formononetin from Trifolium pretense. The analytical results indicate that the imprinted polymers are promising materials for selective identification and enrichment of formononetin in complicated herbal medicines by simple temperature-responsive regulation.
- Published
- 2018
14. Searching for Potential Biomarkers of Penthorum chinense Pursh by Extraction Method and HPLC-MS Analysis Integrated with Evaluation of Hepatoprotective Effect
- Author
-
Chun-Su Yuan, Yu-Zhen Zhang, Zong-Liang Sun, Lian-Di Zhou, Qi-Hui Zhang, Cai-Bing Wei, Chong-Zhi Wang, and Jin-Yi Wan
- Subjects
Chromatography ,Chemistry ,Penthorum chinense ,010401 analytical chemistry ,Biophysics ,Ms analysis ,Pharmaceutical Science ,010402 general chemistry ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,0104 chemical sciences ,Potential biomarkers ,Molecular Medicine ,Extraction methods - Published
- 2018
15. Quality assessment of Penthorum chinense Pursh through multicomponent qualification and fingerprint, chemometric, and antihepatocarcinoma analyses
- Author
-
Guocan Zheng, Chun-Su Yuan, Lian-Di Zhou, Qi-Hui Zhang, Ling Tan, Zong-Liang Sun, Yu-Zhen Zhang, Feng Zhang, Jia-Wei Zhang, and Chong-Zhi Wang
- Subjects
Flavonoids ,0301 basic medicine ,Traditional medicine ,Penthorum chinense ,Quality assessment ,Liver Neoplasms ,General Medicine ,Biology ,Antineoplastic Agents, Phytogenic ,Thonningianin B ,Magnoliopsida ,03 medical and health sciences ,030104 developmental biology ,Fingerprint ,Cell Line, Tumor ,Principal component analysis ,Humans ,Control methods ,Cell Proliferation ,Drugs, Chinese Herbal ,Food Science - Abstract
An efficient method combined with fingerprint and chemometric analyses was developed to evaluate the quality of the traditional Chinese medicine plant Penthorum chinense Pursh. Nine samples were collected from different regions during different harvest periods, and 17 components in the form of extracts were simultaneously examined to assess quality by using high-performance liquid chromatography. The hepatoprotective effects of components were investigated by assessing the inhibition of SMMC-7721 cell growth. The results indicated that the quality control method was accurate, stable, and reliable, and the hierarchical heat-map cluster and the principle component analyses confirmed that the classification of all nine samples was consistent. Quercetin and ellagitannins including pinocembrin-7-O-[3''-O-galloyl-4'',6''-hexahydroxydiphenoyl]-β-glucose (PGHG), thonningianin A, thonningianin B, and other flavonoids were abundant in the extracts, and significantly contributed to the hepatoprotective effects.
- Published
- 2018
16. Quercetin protects mouse liver against triptolide-induced hepatic injury by restoring Th17/Treg balance through Tim-3 and TLR4-MyD88-NF-κB pathway
- Author
-
Chun-Su Yuan, Qi-Hui Zhang, Rong Jiang, Kun Tao, Cai-Bing Wei, and Lian-Di Zhou
- Subjects
0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Immunology ,Biology ,T-Lymphocytes, Regulatory ,Antioxidants ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Immunology and Allergy ,Hepatitis A Virus Cellular Receptor 2 ,Cells, Cultured ,Pharmacology ,Liver injury ,NF-kappa B ,FOXP3 ,Forkhead Transcription Factors ,hemic and immune systems ,NF-κB ,Phenanthrenes ,Triptolide ,medicine.disease ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,030104 developmental biology ,Cytokine ,Endocrinology ,Liver ,chemistry ,Myeloid Differentiation Factor 88 ,TLR4 ,Cancer research ,Epoxy Compounds ,Th17 Cells ,Female ,Quercetin ,Chemical and Drug Induced Liver Injury ,Diterpenes ,Signal transduction ,Signal Transduction - Abstract
Triptolide (TP) is a diterpene triepoxide with various biological activities, but its clinical applications have been limited by potential hepatotoxicity, which can be attributed to T helper 17 (Th17)/T regulatory (Treg) cell imbalance. Quercetin (QE), a natural flavonoid, has been reported to have many benefits and medicinal properties, including hepatoprotective activity against TP-induced liver injury. However, the hepatoprotection mechanisms have not been clarified. The present study was designed to explore the protective effect and the mechanism of QE against TP-induced liver injury. Treatment with QE (20, 50 and 80mg/kg) prior to TP administration restored TP-induced alterations in a certain dose range indicating that QE was able to inhibit TP-induced liver injury. One mechanism underlying this effect was the shifting balance in Th17 and Treg cells from Th17 dominance to Treg dominance. Furthermore, QE markedly decreased the expression level of the Th17-related pro-inflammatory cytokines interleukin (IL)-17 and IL-6, as well as the Th17 transcription factor retinoid-related orphan receptor-γt (ROR-γt). TP induced downregulation in the expression of anti-inflammatory cytokine IL-10, but the expression of Treg transcription factor forkhead/winged-helix family transcriptional repressor p3 (FoxP3) was restored by QE. In the process of exploring the possible hepatoprotective mechanisms of QE, we found that QE significantly reduced both protein and mRNA expression of Toll-like receptor 4 (TLR4), which in turn not only inactivated myeloid differentiation primary response gene 88 (MYD88), nuclear factor kappa B (NF-κB) and related inflammatory cytokines IL-6 and IL-17, but also simultaneously increased the levels of T-cell immunoglobulin and mucin domain-containing protein 3 (Tim-3). Furthermore, blocking of TLR4 enhanced the effect of QE in regulating the Th17/Treg imbalance. In summary, this report has demonstrated for the first time that the protection afforded by QE against TP-induced liver injury was associated with a shift in the balance of Th17 and Treg cells to Treg dominance, which was regulated by Tim-3 and TLR4-MyD88-NF-κB signaling pathway.
- Published
- 2017
17. Investigating two distinct dummy templates molecularly imprinted polymers as paclitaxel adsorbent in synthesis system and releaser in biological samples
- Author
-
Qi-Hui Zhang, Zhuang-Fei Jiang, Li-Li Yang, Chun-Su Yuan, Rong-Rong Ma, Jia-Yuan He, Yan-Jun Li, Ling-Tan, Qing-Yao Li, and Lian-Di Zhou
- Subjects
endocrine system ,Chromatography ,Bulk polymerization ,010401 analytical chemistry ,Extraction (chemistry) ,Molecularly imprinted polymer ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Drug vehicle ,chemistry.chemical_compound ,Adsorption ,Paclitaxel ,chemistry ,Drug delivery ,Side chain ,0210 nano-technology ,Spectroscopy - Abstract
Two distinct new dummy-template molecularly imprinted polymers (D-MIPs) for selective extraction of paclitaxel (PTX) were prepared by the bulk polymerization approach using 10-deacetylbaccatin III (10-DAB) and the side chain (SC) of PTX as the template molecules individually. The obtained D-MIPs exhibited high adsorption capacity and high selectivity: 10-DAB-MIPs for PTX 15.48 mg/g and SC-MIPs for PTX 19.23 mg/g. Under the optimum experimental conditions, both 10-DAB-MIPs and SC-MIPs could be successfully applied to enrich PTX from its structural analogs and acted as a drug vehicle for delivering therapy. For example, 10-DAB-MIPs and SC-MIPs were used as PTX carriers, and the carriers were incubated with artificial gastric juice, artificial intestinal fluid, and patient fecal samples. PTX was detected in samples with an elution rate of about 45%, indicating that the two D-MIPs were good vehicles for delivering PTX in the intestine. The results showed that these D-MIPs had great potential to be used as adsorbing materials for PTX and for drug delivery systems in complex biological systems.
- Published
- 2021
18. Rapid measurements of curcumin from complex samples coupled with magnetic biocompatibility molecularly imprinted polymer using electrochemical detection
- Author
-
Lian-Di Zhou, Jia-Wei Zhang, Shu-Xian Wang, Qi-Hui Zhang, Wei Li, Zhuang-Fei Jiang, Chong-Zhi Wang, Chun-Su Yuan, and Ling Tan
- Subjects
Curcumin ,Biocompatibility ,Polymers ,Surface Properties ,Kinetics ,Filtration and Separation ,Biocompatible Materials ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Molecular Imprinting ,chemistry.chemical_compound ,symbols.namesake ,Adsorption ,Solid phase extraction ,Particle Size ,Magnetite Nanoparticles ,chemistry.chemical_classification ,010405 organic chemistry ,Molecularly imprinted polymer ,Langmuir adsorption model ,Polymer ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,symbols ,0210 nano-technology ,Nuclear chemistry - Abstract
Curcumin widely exists in food, and rapid selective and accurate detection of curcumin have great significance in chemical industry. In this experiment, a new magnetic biocompatibility molecularly imprinted polymer was prepared with nontoxic and biocompatible Zein to adsorb curcumin selectively. The polymer has high biocompatibility, good adsorption capacity, and specific adsorption for curcumin. Combined with portable electrochemical workstations, the polymer can be used to detect curcumin rapidly and cost-effectively. Using curcumin as a template and Zein as the crosslinking agent, the polymers were synthesized on the surface of Fe3 O4 particles for solid phase extraction. The experimental results showed that the polymer reached large adsorption capacity (32.12 mg/g) with fast kinetics (20 min). The adsorption characteristic of the polymer followed the Langmuir isotherm and pseudo-second-order kinetic models. Hexacyanoferrate was used as electrochemical probe to generate signals, and the linear range was 5-200 µg/mL for measuring curcumin. The experimental analysis showed that the polymer was an ideal material for selective accumulation of curcumin from complex samples. This approach has been successfully applied to the determination of curcumin in food samples with electrochemical detection, indicating that this is a feasible and practical technique.
- Published
- 2019
19. Extraction of activated epimedium glycosides in vivo and in vitro by using bifunctional-monomer chitosan magnetic molecularly imprinted polymers and identification by UPLC-Q-TOF-MS
- Author
-
Ri-Fa Qiao, Zhining Xia, Qi-Hui Zhang, Chun-Su Yuan, Jinbin Yuan, Ling Tan, Jia-Wei Zhang, Feng-Qing Yang, Chong-Zhi Wang, and Lian-Di Zhou
- Subjects
Male ,Polymers ,Flavonoid ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Molecular Imprinting ,Chitosan ,chemistry.chemical_compound ,Molecularly Imprinted Polymers ,In vivo ,Animals ,Glycosides ,Epimedium ,chemistry.chemical_classification ,Chromatography ,biology ,Magnetic Phenomena ,Solid Phase Extraction ,010401 analytical chemistry ,Molecularly imprinted polymer ,Glycoside ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Rats ,0104 chemical sciences ,Monomer ,chemistry ,Methacrylic acid ,Adsorption ,0210 nano-technology - Abstract
In this work, efficient, sensitive bifunctional-monomer chitosan magnetic molecularly imprinted polymers (BCMMIPs) were fabricated and successfully applied to concentrate the metabolites of Epimedium flavonoids in rat testis and bone that were later analyzed using UPLC-Q-TOF-MS. Using chitosan and methacrylic acid as co-functional monomers, BCMMIPs exhibited a large adsorption capacity (7.60 mg/g), fast kinetics (60 min), and good selectivity. Chitosan is bio-compatible and non-toxic, and methacrylic acid provides multiple hydrogen bond donors. The BCMMIPs were injected into rat testis to specifically enrich the total flavonoid metabolites in vivo and were used to extract metabolites from bone in vitro. The results showed that the BCMMIPs coupled with UPLC-Q-TOF-MS successfully identified 28 compounds from testis and 18 compounds from bone, including 19 new compounds. This study provided a reliable protocol for the concentration of metabolites from complex biological samples, and several new metabolites of Epimedium flavonoids were found in vivo and in vitro.
- Published
- 2020
20. Simultaneous extraction of several targets by using non-toxic dual template molecularly imprinted polymers in vivo and in vitro
- Author
-
Feng-Qing Yang, Jia-Yuan He, Qi-Hui Zhang, Chong-Zhi Wang, Lian-Di Zhou, Zhining Xia, Jia-Wei Zhang, and Chun-Su Yuan
- Subjects
Polymers ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Molecular Imprinting ,Mice ,chemistry.chemical_compound ,Molecularly Imprinted Polymers ,Pharmacokinetics ,In vivo ,Animals ,heterocyclic compounds ,Chromatography ,biology ,Chemistry ,Solid Phase Extraction ,010401 analytical chemistry ,Extraction (chemistry) ,Molecularly imprinted polymer ,021001 nanoscience & nanotechnology ,biology.organism_classification ,In vitro ,0104 chemical sciences ,Drug delivery ,Adsorption ,0210 nano-technology ,Quercetin ,Schisandra - Abstract
In this report, a non-toxic Dual Template Molecularly Imprinted Polymers (DMIPs) was synthesized with quercetin and schisandrin b as template molecules, using deep-eutectic solvents as functional monomers for the first time. The DMIPs were used to efficiently and simultaneously enrich quercetin and schisandrin b from the mixed crude extracts of penthorum and schisandra. The results indicated that the DMIPs exhibited rapid adsorption kinetics (80 min for adsorption equilibrium) and high selectivity. The largest adsorbing capacities to quercetin and schisandrin b were 23.58 mg/g and 41.64 mg/g, respectively. After presaturation with quercetin and schisandrin b, the nontoxic saturated DMIPs were fed to the mice. Blood samples of the mice were taken and both quercetin and schisandrin b were successfully detected. The pharmacokinetics of quercetin and schisandrin b were similar to reports in the literature where mice were directly fed with botanicals. Our study provides a reliable protocol such that DMIPs can be used to separate and enrich several target molecules simultaneously from complex biological systems. Our findings suggested that the DMIPs have potential application as a drug delivery system of compound herbal formulas.
- Published
- 2020
21. Solid-phase microextraction technology for in vitro and in vivo metabolite analysis
- Author
-
Zhining Xia, Chun-Su Yuan, Hua Chen, Chong-Zhi Wang, Qi-Hui Zhang, and Lian-Di Zhou
- Subjects
Chromatography ,Chemistry ,010401 analytical chemistry ,010501 environmental sciences ,Metabolite analysis ,Solid-phase microextraction ,01 natural sciences ,Article ,In vitro ,0104 chemical sciences ,Analytical Chemistry ,Metabolomics ,In vivo ,Environmental chemistry ,Pharmaceutical sciences ,Spectroscopy ,Combined method ,0105 earth and related environmental sciences - Abstract
Analysis of endogenous metabolites in biological samples may lead to the identification of biomarkers in metabolomics studies. To achieve accurate sample analysis, a combined method of continuous quick sampling and extraction is required for online compound detection. Solid-phase microextraction (SPME) integrates sampling, extraction and concentration into a single solvent-free step for chemical analysis. SPME has a number of advantages, including simplicity, high sensitivity and a relatively non-invasive nature. In this article, we reviewed SPME technology in in vitro and in vivo analyses of metabolites after the ingestion of herbal medicines, foods and pharmaceutical agents. The metabolites of microorganisms in dietary supplements and in the gastrointestinal tract will also be examined. As a promising technology in biomedical and pharmaceutical research, SPME and its future applications will depend on advances in analytical technologies and material science.
- Published
- 2016
22. Epigallocatechin-3-gallate Prevents Triptolide-Induced Hepatic Injury by Restoring the Th17/Treg Balance in Mice
- Author
-
Shu-Jing Yu, Ying Z. Mazzu, Lian-Di Zhou, Yu-Zhen Zhang, Zong-Liang Sun, Rong Jiang, Qi-Hui Zhang, and Cai-Bing Wei
- Subjects
0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Inflammation ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,Internal medicine ,medicine ,Interleukin 6 ,Liver injury ,biology ,food and beverages ,General Medicine ,Triptolide ,medicine.disease ,Interleukin 10 ,030104 developmental biology ,Endocrinology ,Cytokine ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,TLR4 ,medicine.symptom - Abstract
Drug-induced liver injury (DILI) is the most common cause of acute liver failure. Disruption of the Th17/Treg balance can lead to hepatic inflammation, which causes the main symptoms of DILI. Here we investigate the protective mechanisms of (-)-Epigallocatechin-3-gallate (EGCG) on triptolide (TP)-induced DILI that shows the Th17/Treg imbalance. Pretreatment with EGCG (5[Formula: see text]mg/kg) for 10 days before TP (0.5[Formula: see text]mg/kg) administration in mice significantly reduced the increased alanine aminotransferase (ALT) level ([Formula: see text]) induced by TP treatment. The hepatic histology analysis further proved that EGCG protected mice from TP-induced liver injury. The imbalance of Th17/Treg was induced by TP treatment, as shown by the upregulation of TLR4 and downregulation of Tim3 expression. EGCG pretreatment can maintain the expression of TLR4 and Tim3 at normal levels to restore the Th17/Treg imbalance. In addition, EGCG can block the TP-induced expression of the downstream targets of TLR4, including MyD88, NF[Formula: see text]B, and retinoid related orphan receptor (ROR-[Formula: see text]t), while EGCG can restore the TP inhibition of forkhead/winged-helix family transcriptional repressor p3 (FoxP3) that is the downstream target of Tim3. Consequently, EGCG pretreatment can effectively inhibit the Th17-related pro-inflammatory cytokine (e.g. IL-17 and IL-6) upregulation induced by TP treatment. However, TP inhibition of Treg-related anti-inflammatory cytokine IL-10 production was restored by EGCG pretreatment. Taken together, these results suggest that EGCG possesses significant protective properties against TP-induced hepatic inflammatory injury, and that these properties are carried out via the restoration of the Th17/Treg imbalance by the inhibition of the TLR4 signaling pathway and the enhanced activation of the Tim3 signaling pathway.
- Published
- 2016
23. Specific adsorption of tetracycline from milk by using biocompatible magnetic molecular imprinting material and evaluation by ECD
- Author
-
Chun-Su Yuan, Rong-Rong Ma, Ling Tan, Zhining Xia, Qi-Hui Zhang, Lian-Di Zhou, Shu-Xian Wang, Jia-Wei Zhang, Wei Li, and Ying Z. Mazzu
- Subjects
Materials science ,Polymers ,Scanning electron microscope ,Zein ,Biocompatible Materials ,Food Contamination ,01 natural sciences ,Analytical Chemistry ,Molecular Imprinting ,symbols.namesake ,0404 agricultural biotechnology ,Adsorption ,X-Ray Diffraction ,Limit of Detection ,Animals ,Solid phase extraction ,Magnetite Nanoparticles ,chemistry.chemical_classification ,Detection limit ,Magnetic Phenomena ,010401 analytical chemistry ,Molecularly imprinted polymer ,Langmuir adsorption model ,Electrochemical Techniques ,04 agricultural and veterinary sciences ,General Medicine ,Polymer ,Tetracycline ,040401 food science ,Anti-Bacterial Agents ,0104 chemical sciences ,Milk ,chemistry ,Tetracyclines ,symbols ,Molecular imprinting ,Food Analysis ,Food Science ,Nuclear chemistry - Abstract
Biocompatible magnetic molecularly imprinted polymers (BMMIPs) were prepared with Zein for the first time, and were used to enrich tetracycline compounds selectively. Innovative combination of BMMIPs and electrochemistry to obtain lower detection line to satisfy industrial detection demands. Using Zein as the crosslinking agent, the polymers were synthesized on the surface of Fe3O4 particles. The scanning electron microscope, transmission electron microscope and X-ray diffraction technologies were used to characterize BMMIPs. Through optimization, BMMIPs attained large adsorption capacity (236.40 mg/g) with fast kinetics (40 min) and followed the Langmuir isotherm and pseudo-second-order kinetic models. BMMIPs had good recognition ability, the selective factors of oxytetracycline, chlortetracycline, doxycycline were 4.78, 4.23, and 3.39, respectively. Excellent linearity was attained in the range of 0.025–500 μg/mL, with low detection limits and low quantitation limits of 0.025 and 0.083 μg/mL. According to our exploring, BMMIPs was ideal materials for enrichment of tetracycline in complex biological samples.
- Published
- 2020
24. 6,8-di-C-glycosyl flavones with β-furanoarabinose from Scutellaria baicalensis and their anti-inflammatory activities
- Author
-
Chun-Su Yuan, Lian-Di Zhou, Qian Gou, Ling Tan, Chong-Zhi Wang, and Qi-Hui Zhang
- Subjects
Lipopolysaccharides ,Lipopolysaccharide ,medicine.drug_class ,Anti-Inflammatory Agents ,Inflammation ,Plant Science ,01 natural sciences ,Biochemistry ,Flavones ,Anti-inflammatory ,Dinoprostone ,Analytical Chemistry ,chemistry.chemical_compound ,Mice ,medicine ,Macrophage ,Animals ,Glycosyl ,chemistry.chemical_classification ,biology ,010405 organic chemistry ,Chemistry ,Interleukin-6 ,Organic Chemistry ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,RAW 264.7 Cells ,Scutellaria baicalensis ,medicine.symptom ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Two flavone di-C-glycosides, a pair of isomers, were isolated from Scutellaria baicalensis. The structures of compounds 1 and 2 were elucidated by means of physical data, including 1D and 2D NMR and HR-ESI-MS. Supporting theoretical calculations of the compound conformational landscape has also been conducted for geometry optimization. This is the first report of the natural occurrence of β-furanoarabinoside. In addition, the effects of compounds 1 and 2 on NO, pro-inflammatory cytokines, PGE2 and COX-2 levels were measured in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. The pair of isomers exhibited significant inhibitory effects on inflammation.
- Published
- 2018
25. Polydopamine-Coated Magnetic Molecularly Imprinted Polymers with Fragment Template for Identification of Pulsatilla Saponin Metabolites in Rat Feces with UPLC-Q-TOF-MS
- Author
-
Chong-Zhi Wang, Chun-Su Yuan, Jia-Wei Zhang, Yu-Zhen Zhang, Lian-Di Zhou, and Qi-Hui Zhang
- Subjects
Indoles ,Polymers ,Saponin ,02 engineering and technology ,Mass spectrometry ,01 natural sciences ,Mass Spectrometry ,Matrix (chemical analysis) ,Molecular Imprinting ,Feces ,Magnetics ,Adsorption ,Animals ,Solid phase extraction ,Magnetite Nanoparticles ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Chromatography ,Chemistry ,010401 analytical chemistry ,Extraction (chemistry) ,Solid Phase Extraction ,Molecularly imprinted polymer ,General Chemistry ,Saponins ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Rats ,Pulsatilla ,0210 nano-technology ,General Agricultural and Biological Sciences ,Molecular imprinting - Abstract
In this work, a modified pretreatment method using magnetic molecularly imprinted polymers (MMIPs) was successfully applied to study the metabolites of an important botanical with ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The MMIPs for glucoside-specific adsorption was used to identify metabolites of Pulsatilla chinensis in rat feces. Polymers were prepared by using Fe3O4 nanoparticles as the supporting matrix, d-glucose as fragment template, and dopamine as the functional monomer and cross-linker. Results showed that MMIPs exhibited excellent extraction performance, large adsorption capacity (5.65 mg/g), fast kinetics (60 min), and magnetic separation. Furthermore, the MMIPs coupled with UPLC-Q-TOF-MS were successfully utilized for the identification of 17 compounds including 15 metabolites from the Pulsatilla saponin metabolic pool. This study provides a reliable protocol for the separation and identification of saponin metabolites in a complex biological sample, including those from herbal medicines.
- Published
- 2017
26. Spatial Pattern Analysis of Land Use and Land Cover Change Based on MODIS
- Author
-
Hong Li, Shi Wei Dong, Dan Feng Sun, Wei Wei Zhang, and Lian Di Zhou
- Subjects
Land use ,business.industry ,Environmental resource management ,General Engineering ,Land cover ,Geography ,Beijing ,Land information system ,Remote sensing (archaeology) ,Common spatial pattern ,Land development ,business ,China ,Remote sensing - Abstract
Understanding the process and pattern of regional land use and land cover change was essential for intensive and highly efficient, scientific and rational utilization of land resources. Technologies of remote sensing (RS) and geographical information system (GIS) were applied to simulate and analyze the land use changes from 2000 to 2007 in Pinggu district in Beijing, China. The area of cropland decreased by 62.42 km2, while the area of construction land, orchards and forests increased 17.65 km2, 25.88 km2 and 17.86 km2, respectively. We should develop appropriate policies and measures to increase the area of cultivated land in the future. The research results provided the necessary reference for the related researches of land use and land cover change.
- Published
- 2013
27. Application Research of Remote Sensing in the Field of Land Use and Land Cover
- Author
-
Lian Di Zhou, Shi Wei Dong, Dan Feng Sun, Wei Wei Zhang, and Hong Li
- Subjects
ComputingMilieux_GENERAL ,Land information system ,Land use ,Remote sensing (archaeology) ,General Engineering ,Land management ,Land consolidation ,Environmental science ,Land cover ,Temporal scales ,Field (geography) ,Remote sensing - Abstract
As a new high-technology with large amount of information, strong temporal resolution, high efficiency and low cost, remote sensing provided a useful tool for related researches of land use and land cover in different spatial and temporal scales. Firstly, concepts and characteristics of the remote sensing technology were introduced. Secondly, its applications were elaborated in land use and land cover such as land resources survey, land resources change monitoring, land use evaluation, overall planning of land use and land consolidation aspects. At last, future application trends and several aspects noticed were pointed out.
- Published
- 2013
28. Valley Economy Regionalization Based on Geographical Information System and Statistical Analysis: A Case of Beijing Mountainous Area
- Author
-
Feng He, Lian Di Zhou, Dan Feng Sun, and Hong Li
- Subjects
Production area ,Geography ,Economy ,Beijing ,Water source ,General Engineering ,Information system ,Statistical analysis ,Scale (map) - Abstract
This paper discussed the regionalization of valley economy of the mountainous area of Beijing. 19 indexes of natural ecological and socio-economic systems were covered,and valley were considered as complete individual taxons. The mountainous area of Beijing was divided into 4 valley economic regions : Shallow mountain, plain grain and vegetable production area (I), Plain premier, special and high quality varieties planting area (II), Remote mountains forest conservation zone (III), Suburban Hilly Area and Remote Mountains ,water source protection and forestry product planting area (IV) valley economic regions by Principal Component Analysis (PCA) and Cluster Analysis (CA) combining with Geographical Information System (GIS) platform. These 4 regions were further divided into 12 valley economic types. The conclusion analyzed the regionalization and methodological framework for large scale areas like the mountainous area of Beijing. Such practice could be generalized to develop appropriate regional valley economic theory for large scale areas.
- Published
- 2013
29. Spectral Characteristics of Cr and Cu in Agricultural Soil at Beijing Plain Based on SWIR Spectroscopy and PLSR
- Author
-
Hong Li, Shu Min Li, Lian Di Zhou, and Dan Feng Sun
- Subjects
Beijing ,Linear regression ,Soil water ,Outlier ,Partial least squares regression ,General Engineering ,Environmental science ,Heavy metals ,Soil science ,Spectroscopy ,Spectral line ,Remote sensing - Abstract
Heavy metals pollution in agricultural soils has been an important problem to human health, mapping large-scale spatial distribution of soil heavy metals is urgently needed. Instead of traditional methods, time-consuming and destructive, soil properties predicted by remote sensing technology shows a lot of advantages, which makes large area of real-time dynamic monitoring as possible. However, before achieving prediction using spectra data, the first thing to do is that finding the spectral characteristics of soil heavy metals. In this paper, taking Cr and Cu for example, the correlations between soil heavy metals content and laboratory-measured reflectance is studied using partial least squares regression (PLSR), which is an adaptive method to examine linear between spectrum and concentration. First of all, using the raw spectra, remove outliers of heavy metals concentration by PLSR modeling. Next, though comparing RMSEC and RMSEV against PLSR components, and cumulative explanatory of spectral components to metal content using different pre-precessing methods, find the right pre-pcocessing is CR and optimum number of components to Cr and Cu are 3 and 2 respectively. Simultaneously, with the meaning of PLSR models regression coefficients, we analysis the spectral characteristics of Cr and Cu, although can not to realize the prediction only take use of these spectra, which is still essential to achieve simulating spatial distribution of soil heavy metal by remote sensing.
- Published
- 2013
30. Fourier and Wavelet Analysis of Water Quality Signals
- Author
-
Lian Di Zhou, Dan Feng Sun, Wei Zhang, and Hong Li
- Subjects
symbols.namesake ,Watershed ,Wavelet ,Fourier transform ,Fourier analysis ,Surface water quality ,General Engineering ,symbols ,Water quality ,Temporal change ,Scale (map) ,Geology ,Remote sensing - Abstract
Taking the nitrogen monitoring data of two sites S1 and S2 in the period 1988-2003 in Baihe River lying Miyun reservoir stream watershed as a case, Fourier and Wavelet analysis were adopted to explore and compare the periodic patterns and temporal pattern characteristics of the two sites. The results showed that the periodic patterns of two sites were discovered using Fourier analysis. The site S1 had a period of two years, while the site S2 had no significant periodic patterns. The temporal pattern characteristics at different scales were obtained through wavelet analysis, which were at small scale for the site S1, while at moderate and small scales for the site S2. The Fourier and wavelet analysis method can both be used in the study of surface water quality temporal change pattern, the first is a coarse method and the latter is a more detailed method for analyzing surface water quality temporal pattern characteristics.
- Published
- 2013
31. The Effect on Soil Microbial Biomass and Soil Nutrient of Interplanted in Chestnut Forest
- Author
-
Zhi Chen Yang, Jin Shun Bai, Lian Di Zhou, and Hong Li
- Subjects
biology ,Microorganism ,Soil organic matter ,Phosphorus ,fungi ,General Engineering ,food and beverages ,Biomass ,chemistry.chemical_element ,biology.organism_classification ,complex mixtures ,Crop ,Tillage ,Nutrient ,Agronomy ,chemistry ,Environmental science ,Agropyron - Abstract
By analyzing the different crop interplanting test in chestnut forests, the results show that soil nitrate nitrogen, ammonium nitrogen and available phosphorus fell in October when intercropped plants, this may be related to the growth of chestnut trees absorb nutrients and microorganisms in the soil. The results of this study show that interplanting different plants had an impact on soil microbial biomass carbon and microbial biomass phosphorus. Microbial biomass carbon turnover rate in the order: ryegrass> clean tillage> natural grass> Alfalfa> flat Agropyron> soybean; turnover rates of soil microbial biomass phosphorus in the order: clean tillage> Alfalfa> ryegrass natural grass> soy> flat crested wheatgrass.
- Published
- 2013
32. Analysis of the Main Factors of Vegetable Circulation Efficiency in Beijing Based on the Analytic Hierarchy Process
- Author
-
Lian Di Zhou, Ai Sheng Ren, and Peng Jiang
- Subjects
Engineering ,Beijing ,Operations research ,Influence factor ,business.industry ,Rank (computer programming) ,Analytic hierarchy process ,Circulation (currency) ,General Medicine ,business - Abstract
For the characteristics of numerous populations, low self-sufficiency rate of vegetables, this article studies how to improve the supply ability of vegetables in Beijing by enhancing vegetable circulation efficiency. In this paper, we rank the weights of factors which are calculated by the analytic hierarchy process (AHP) in vegetable circulation, analyze the main factors in the evaluation results, and then provide the methods of solving problems.
- Published
- 2013
33. Strategy for Renewable Energy Development in Rural Areas of Beijing
- Author
-
Lian Di Zhou, Di Tang Su, and Zhong Ren Zhou
- Subjects
Engineering ,business.industry ,media_common.quotation_subject ,General Engineering ,Environmental engineering ,Environmental economics ,Solar energy ,Energy engineering ,Renewable energy ,Beijing ,Service (economics) ,Product (category theory) ,Rural area ,business ,Feed-in tariff ,media_common - Abstract
Beijing has long attached great importance to the development of renewable energy in its rural areas. On the basis of an analysis of resources, history, and status, this study presents a strategy for developing renewable energy in rural Beijing. Namely, for Beijing to become a high-level demonstration zone for renewable energy use, it must grasp two key development links (renewable energy and energy saving), focus on three strategic emphases (solar energy heat utilization, biomass gas, and energy-saving housing renovation), and enforce six construction elements (e.g., product research and development, technology upgrades, demonstration zones, and service systems). We also introduce concrete measures for promoting the development of rural renewable energy in Beijing in terms of funds, markets, policies, technologies, and services.
- Published
- 2013
34. Proposal for Developing Carbon Sequestration Economy in Ecological Conservation Area of Beijing
- Author
-
Miao Yu, Dan Feng Sun, Zhi Chen Yang, Hong Li, and Lian Di Zhou
- Subjects
Sustainable development ,Functional ecology ,Government ,Economy ,Beijing ,Ecology ,Urban planning ,Capital (economics) ,General Engineering ,Kyoto Protocol ,Business ,Carbon sequestration - Abstract
In order to build “Culture-enriched Beijing, science and technology Beijing, green city of Beijing” and aim for world cities, the government of Beijing putting in a lot of money to support the ecological environmental protection as the main features of the eco-industrial development, and the city is divided into four functional areas, including the core area of the capital function, Urban Fringe’s Sustainable Development, urban development zone, ecological conservation area, ecological conservation area mainly in the suburbs of Beijing. At present, how to Implement the ecological function and maintain rapid growth in economy of Ecological Conservation Area to guarantee economic health, balanced, sustainable development is a serious problem. This article discusses the approach of "Kyoto Protocol" carbon trading and put forward the implementation of Carbon sequestration economy to solve the contradiction of ecosystem conservation and economic development in Beijing mountain area, by this way we can provide some ideas to construct “Culture-enriched Beijing, science and technology Beijing, green city of Beijing”
- Published
- 2012
35. The Current State and Future Strategy for Utilizing Biomass Energy in Beijing
- Author
-
Qun Chen, Ai Ling Wang, Lian Di Zhou, and Zhong Ren Zhou
- Subjects
Engineering ,Resource (biology) ,Beijing ,business.industry ,Energy (esotericism) ,General Engineering ,Biomass ,Environmental economics ,business ,Livelihood ,Civil engineering - Abstract
In recent years, the biomass energy industry has become an important part of the construction of “Green Beijing” and has begun to grow. This paper analyzes the resource availability, present situation, and existing problems with biomass energy in Beijing. In addition, it examines the advantages and disadvantages of developing biomass energy in Beijing, and proposes future development strategies. The findings are: 1) developing and utilizing biomass energy effectively and appropriately in suburban Beijing to improve livelihoods and the environment; 2) making full use of the technological, knowledge, and information advantages in Beijing by building an R&D base for biomass energy technologies; and 3) funds and technology should be transferred externally to establish a biomass energy industry base in regions outside of Beijing.
- Published
- 2012
36. Long-Term Effects of Crop Residual and Inorganic Fertilizers on Yield and Soil Organic Matter for a Winter Wheat-Maize System in North China Plain
- Author
-
Lian Di Zhou, Hong Li, Zhi Chen Yang, and Yi Zhong Lv
- Subjects
Crop ,Nutrient ,Agronomy ,Agriculture ,business.industry ,Soil organic matter ,Crop yield ,Yield (wine) ,General Engineering ,Environmental science ,Soil carbon ,Soil fertility ,business - Abstract
Long-term effects of inorganic and corn stalks organic nutrient sources on yield, soil organic carbon(SOC) and the relationship between crop yield and SOC were investigated in Dry-Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences, Hengshui, Hebei Province, China from 1981 to 2005. Hengshui is a part the North China Plain and the dominant cropping systems are Winter wheat-maize rotations. Recently the use of organic manure with grain crops has almost disappeared. This could reduce soil fertility and crop productivity in the long run. There were sixteen treatments applied to both wheat and maize seasons: Inorganic fertilizers as main plots and corn stalks as subplots and the main plot and subplot all have four levels expressed A and B respectively. So there are sixteen treatments with three replicates were set (A1,A2,A3,A4)*(B1,B2,B3,B4). After more than 20 years crop yield and SOC decreased in treatment of without fertilizers and only applicate corn stalks just sustain it. Combine application of inorganic fertilizers and corn stalks increase SOC and crop yield very strongly. For crop yield the more fertlizers the more increase. But the SOC will decrease as the more inorganiac fertlizer application and increase with the increase of corn stalks.
- Published
- 2011
37. Study on Economic Development Model and Approach of Beijing's Mountainous Valley Areas
- Author
-
Jun-Hong Chen, Lian-di Zhou, and mei Zhu
- Subjects
Valley Areas ,Economic growth ,Government ,Economic development model of Valley Areas ,Building Thinking ,Space (commercial competition) ,Geography ,Safeguard ,Beijing ,Order (exchange) ,Mountainous Areas ,General Earth and Planetary Sciences ,General Environmental Science - Abstract
The economic development model of Mountainous Valley areas was put forward by Beijing government in order to fit in with the needs of ecosystems conserve and socio-economic development in mountain areas. It had achieved remarkable results in practice. The basic content and the problems faced by the model were both described in this paper. Based on theory of regional economic development, the paper proposed the development thought of “multi-point and one line” and “A line with multi-center” space layout and relative safeguard measures of valley areas. This research can provide reference for relevant departments to formulate economic development policies.
- Published
- 2011
38. Serologic study on the outbreak of acute upper respiratory tract infections caused by adenovirus 3
- Author
-
Liwen Ju, Renjie Jiang, Shunzhang Yu, Lu-Fang Jiang, Yu-zun Lin, Lian-di Zhou, and Qingwu Jiang
- Subjects
Titer ,Respiratory tract infections ,biology.protein ,Outbreak ,General Medicine ,Biology ,Antibody ,Direct fluorescent antibody ,Virology ,Neutralization ,Immunoglobulin G ,Serology - Abstract
From April to June, 2004, an outbreak of acute upper respiratory tract infections (AURTI) occurred in the north area of Jiangsu Province, China. Twenty throat swabs were collected with 13 of them presenting an adenovirus (Ad)-like cytopathogenic effect on HEp-2. These were verified as Ad by the electron microscope, direct immunofluorescence assay and Ad primer-mediated PCR. Moreover, they were identified as adenovirus type 3 (Ad3) by type-specific PCR and sequencing of the amplification products. Subsequent serologic studies were carried out to finally diagnose and document the outbreak. The neutralization test of paired serum of six in nine cases show obviously increased antibodies titers. The positive rate of IgM, IgG and recovery phase neutralization antibodies of the cases were 3.7%, 44.4% and 59.5% respectively while those of the controls were 0%, 8.3% and 33.3% respectively. The P values of Chi-Square were 0.510, 0.018 and 0.226 respectively. The concordance between IgG detected by ELISA and neutralization anti bodies detected by the neutralization test was 61.4% and the P value of Kappa was 0.070. By the serologic study, we can definitively diagnose that this outbreak of acute respiratory infections was caused by Adenovirus 3.
- Published
- 2014
39. Biotransformation and metabolic profile of anemoside B4 with rat small and large intestine microflora by ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry
- Author
-
Lian-Di Zhou, Yu-Zhen Zhang, Feng-Qing Yang, Jin-Bin Yuan, Jinyi Wan, Qi-Hui Zhang, and Yan Chen
- Subjects
0301 basic medicine ,Clinical Biochemistry ,Tandem mass spectrometry ,Biochemistry ,Analytical Chemistry ,HeLa ,03 medical and health sciences ,0302 clinical medicine ,Biotransformation ,In vivo ,Drug Discovery ,medicine ,Large intestine ,MTT assay ,Molecular Biology ,Pharmacology ,Chromatography ,biology ,Chemistry ,General Medicine ,Metabolism ,biology.organism_classification ,Metabolic pathway ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis - Abstract
Pulsatilla chinensis (Bunge) Regel has been commonly used in Asia, and Anemoside B4 conducted as its major saponin with diverse pharmaceutical effects. Previous studies showed that intestinal flora plays an important role in the metabolism of herbs administered orally. In this study, the metabolic profile of AB4 with microflora in rat small and large intestine in vitro was investigated. Gut microflora was collected from different intestinal segments and anaerobically incubated with AB4 at 37 °C for 24, 48, 72 and 96 h, respectively. A total of 10 metabolites were detected and identified by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry, involving the products of oxygenation and deglycosylation reactions. Gut microflora in the large intestine generated more comprehensive metabolic pathways, which appears to attribute to the wider assortment of bacterial types and amount. Human cancer cell lines SMMC-7721, Hela and MCF-7 were treated with metabolites pool by MTT assay, together with M6 as the most deglycosylation product. As a result, M6 exhibited a reduction in cell viability of SMMC-7721 with an IC50 value of 22.28 ± 1.26 μg/mL. The present study provided scientific evidences for AB4 metabolism in small and large intestine, which is helpful to reveal the active forms of AB4 in vivo. This article is protected by copyright. All rights reserved.
- Published
- 2016
40. Polydopamine-Coated Magnetic Molecularly Imprinted Polymers with Fragment Template for Identification of Pulsatilla Saponin Metabolites in Rat Feces with UPLC-Q-TOF-MS.
- Author
-
Yu-Zhen Zhang, Jia-Wei Zhang, Chong-Zhi Wang, Lian-Di Zhou, Qi-Hui Zhang, and Chun-Su Yuan
- Published
- 2018
- Full Text
- View/download PDF
41. Heavy Metals in Greenhouse Vegetable Soils in Beijing: Accumulation Characteristics of Copper and Cadmium
- Author
-
Hong Li, Zhi Wang, Lian-di Zhou, and Hui-zhi Zhang
- Subjects
Cadmium ,chemistry ,Agronomy ,Soil test ,Phosphorus ,Soil water ,chemistry.chemical_element ,Environmental science ,Greenhouse ,Soil carbon ,complex mixtures ,Soil contamination ,Copper - Abstract
A survey was conducted to determine the status of copper and cadmium accumulations in greenhouse vegetable soils in Tongzhou of Beijing. Based on the investigated results, the contamination degree of the two heavy metals in the greenhouse vegetable soils was assessed and the main influence factors caused the accumulation of copper and cadmium was analyzed. The results indicated that there was a distinct increase of copper and cadmium concentration in greenhouse vegetable soils. The concentrations of copper and cadmium in part of soil samples exceeded the environmental quality evaluation standard for farmland of greenhouse vegetables production of China. These over-standard soil samples were mainly collected from Lucheng town and Xiji town. The contents of copper and cadmium in greenhouse soil were increased significantly as the cultivated time of the soil added. The content of copper in greenhouse soil varied from 19.8 mg/kg to 39.2 mg/kg when the cultivation time of the greenhouse increased from 1 year to 5 years, nearly increased one times. The content of cadmium in vegetable soils of 20-year age greenhouse was 3.2 times of that in 1-year age greenhouse. Significant correlations were found between the content of copper and cadmium in soil, soil organic carbon content, CEC, and available phosphorus and potassium content in soils. Soil physical and chemical properties should be considerate integrated to reduce the heavy metal contamination in greenhouse soils.
- Published
- 2012
42. [The variability of vegetation beginning date of greenness period in spring in the north-south transect of eastern China based on NOAA NDVI]
- Author
-
Zhi, Wang, Shi-rong, Liu, Peng-sen, Sun, Zhi-hua, Guo, and Lian-di, Zhou
- Subjects
China ,Spatio-Temporal Analysis ,Agriculture ,Seasons ,Plants ,Environmental Monitoring - Abstract
NDVI based on NOAA/AVHRR from 1982 to 2003 are used to monitor variable rules for the growing season in spring of vegetation in the north-south transect of eastern China (NSTEC). The following, mainly, are included: (1) The changing speed of greenness period in spring of most regions in NSTEC is slow and correlation with the year is not distinct; (2) The regions in which greenness period in spring distinctly change mainly presented an advance; (3) The regions in which inter-annual fluctuation of greenness period in spring is over 10 days were found in 3 kinds of areas: the area covered with agricultural vegetation types; the areas covered with evergreen vegetation types; the areas covered with steppe vegetation types; (4) changes of vegetation greenness period in spring have spatio-temporal patterns.
- Published
- 2010
43. [Surface water quality assessment in Miyun reservoir watershed, Beijing in the period 1980-2003]
- Author
-
Wei-wei, Zhang, Dan-feng, Sun, Hong, Li, and Lian-di, Zhou
- Subjects
China ,Nitrogen ,Water Supply ,Fresh Water ,Phosphorus ,Water Pollutants ,Mercury ,Environmental Monitoring - Abstract
Single factor water quality identification index was adopted to assess the surface water quality of Miyun reservoir watershed in Beijing using nearly 20 years monitoring data of 4 sites, also the surface water quality pollution sources were analyzed. The results indicated TP had the largest temporal variation at every monitoring site, coefficients of variation were 93.86%, 86.08%, 50.56% and 139.47%, respectively. The following element was Hg, the coefficients of its variation were 86.08%, 25.75%, 56.52% and 47.01%, respectively. While TN, permanganate index, BOD5, Pb and Cr were relatively stable with small coefficient of temporal variation. The permanganate index, BOD5, Pb and Cr did not exceed to the Chinese surface drinking water standard limit in the study period, while Hg had high pollution risk in several years, such as monitoring sites S1 and S3 in 1992, monitoring sites S4 in 1996. The major pollutants of Miyun reservoir watershed in Beijing were TN and TP, and TN had larger pollution risk compared with TP in most years. Comparing to that before the 1990s, the decade average fertilizer, pesticide and agricultural plastic mulch inputs after the 1990s had increased by 46%, 173% and 359%, respectively. The husbandry proportion in agriculture rose from 24.4% to 39.8%, and the average gross industrial production by 424%. The upstream of Miyun reservoir had larger pollution risk than its downstream. In addition, Chaohe watershed contributed more TN and TP to the reservoir than Baihe watershed.
- Published
- 2010
44. [Spectral characteristics analysis and remote sensing inversion of water quality parameters in Han Shiqiao wetland]
- Author
-
Wei-Jing, Du, Shu-Min, Li, Hong, Li, Dan-Feng, Sun, and Lian-Di, Zhou
- Abstract
The research object of the present paper is the water quality of Han Shiqiao wetland water. Water spectrum and quality parameters were measured on the site and in the lab. The authors simulated the relationships between water quality parameters and the best bands or combination, and built the multiple linear regression equation to obtain characteristic spectrum of the key water quality parameters. Besides, several key issues involved in applying ASTER satellite imagery to water quality include atmospheric correction, discussing methods for ASTER data bands analysis, and choosing the best bands and band combination. Results indicated that although the simulation model is not universal, the analysis of spectral characteristics based on ground spectrometer could provide foundations for the choice of remote sensing characteristics bands. The band ratio of water quality parameters simulated from ASTER spectral characteristics moves to relatively long-wave band. Finally, based on the analysis of ASTER remote sensing characteristics bands, the authors built water quality parameters regression model. The models for water quality parameters were recommended, and the accuracies of these models were analyzed. Making use of regression model, we executed spatial distribution map of water quality parameters to achieve wetland water monitoring with remote sensing in terms of variation in space and with time.
- Published
- 2010
45. Two new compounds from the marine Nigrospora sphaerica
- Author
-
Li Tian, Hui-Ming Hua, Zhi-Feng Li, Ying Zhang, Qi-Hui Zhang, Lian-Di Zhou, and Yue-Hu Pei
- Subjects
Pharmacology ,chemistry.chemical_classification ,Molecular Structure ,Phenylpropionates ,Chemistry ,Stereochemistry ,Organic Chemistry ,Pharmaceutical Science ,Marine Biology ,General Medicine ,Nigrospora sphaerica ,Analytical Chemistry ,3-hydroxybutan-2-yl 2-hydroxy-3-phenylpropanoate ,medicine.drug_formulation_ingredient ,Complementary and alternative medicine ,Ascomycota ,Drug Discovery ,medicine ,Molecular Medicine ,Organic chemistry ,Benzeneethanol ,Nuclear Magnetic Resonance, Biomolecular ,Lactone ,Phenylacetates - Abstract
Two new compounds, 1-(5-oxotetrahydrofuran-2-yl)ethyl 2-phenylacetate (1) and 3-hydroxybutan-2-yl 2-hydroxy-3-phenylpropanoate (2), along with three known compounds, harzialactone A (3), benzeneethanol 4-hydroxy-1-acetate (4), and 1,4-dioxane-2,5-dione-3,6-bis(penylmethyl)-homopolymer (5), have been isolated from the fungus Nigrospora sphaerica. Their structures were determined on the basis of chemical and spectroscopic methods.
- Published
- 2010
46. [Estimation of regional leaf area index by remote sensing inversion of PROSAIL canopy spectral model]
- Author
-
Shu-Min, Li, Hong, Li, Dan-Feng, Sun, and Lian-Di, Zhou
- Abstract
The present paper selected Qing yundian town and Weishanzhuang town in Da Xing District, and Gaoling ying town in Shunyi District as test areas, using MODIS data and ASTER data in different scales. The feasibility of winter wheat LAI inversion by PROSAIL physical model, especially the stability of remote sensing data in different scales, was discussed, and the results from experience model inversion were compared with that from statistical methods. The values of all samples LAI inversion from experience model are close in a region, which means experience model is a reflection of general growing trend, ignoring spatial heterogeneity of the regional leaf area index. But the value of LAI inversion from physical model can be truer in reflecting spatial heterogeneity of the regional leaf area index. The value of LAI inversion from physical model is more real, compared with experience model. With the method of linear weighing, the scale conversion was accomplished, and the LAI inversion results from different remote sensing scale data were compared, and were found similar. The result shows that in the process of large-scale regional LAI inversion, physical model inversion is more valid.
- Published
- 2009
47. The shiitake mushroom-derived immuno-stimulant lentinan protects against murine malaria blood-stage infection by evoking adaptive immune-responses
- Author
-
Jun Liu, Lian-di Zhou, Ying Zhang, Yaming Cao, and Qi-hui Zhang
- Subjects
medicine.drug_class ,T cell ,Immunology ,Lentinan ,Shiitake Mushrooms ,chemical and pharmacologic phenomena ,Apoptosis ,Biology ,Nitric Oxide ,Parasitemia ,Immunostimulant ,T-Lymphocytes, Regulatory ,Microbiology ,chemistry.chemical_compound ,Interferon-gamma ,Mice ,Immune system ,Adjuvants, Immunologic ,parasitic diseases ,medicine ,Immunology and Allergy ,Animals ,IL-2 receptor ,Cells, Cultured ,Pharmacology ,Mice, Inbred BALB C ,Histocompatibility Antigens Class II ,Dendritic Cells ,Plasmodium yoelii ,Th1 Cells ,biology.organism_classification ,Interleukin-12 ,Toll-Like Receptor 2 ,Malaria ,Toll-Like Receptor 4 ,TLR2 ,Disease Models, Animal ,medicine.anatomical_structure ,chemistry ,Interleukin 12 ,B7-1 Antigen ,Female ,B7-2 Antigen - Abstract
Lentinan, a (1-3)-beta glucan from Lentinus edodes, is an effective immunostimulatory drug. We tested the effects of lentinan during blood-stage infection by Plasmodium yoelii 17XL (P.y17XL). Pre-treatment of mice with lentinan significantly decreased the parasitemia and increased their survival after infection. Enhanced IL-12, IFN-gamma and NO production induced by lentinan in spleen cells of infected mice revealed that the Th1 immune response was stimulated against malaria infection. In vitro and in vivo, lentinan can result in enhanced expression of MHC II, CD80/CD86, and Toll-like receptors (TLR2/TLR4), and increased production of IL-12 in spleen dendritic cells (DCs) co-cultured with parasitized red blood cells (pRBCs). Moreover, both the number of CD4(+)CD25(+) regulatory T cells (Tregs) and the levels of IL-10 secreted by Tregs were reduced by pre-treatment with lentinan in the spleen of malaria-infected mice. Meanwhile, apoptosis of CD4(+) T cell in spleens of mice pretreated with lentinan was significantly reduced. In summary, lentinan can induce protective Th1 immune responses to control the proliferation of malaria parasites during the blood-stage of P.y17XL infection by stimulating maturation of DCs to inhibit negative regulation of the Th1 immune response by Tregs. Taken together, our findings suggest that lentinan has prophylactic potential for the treatment of malaria.
- Published
- 2008
48. [Sequence analysis on measles viruses isolated in Shanghai in 2005]
- Author
-
Shu-hua, Li, Zheng, Ni, Li-wen, Ju, Hui-guo, Shen, Yi-yun, Tan, Lu-fang, Jiang, Lian-di, Zhou, Yu-zun, Lin, Ying-jie, Zheng, and Qing-wu, Jiang
- Subjects
China ,Genotype ,Measles virus ,Reverse Transcriptase Polymerase Chain Reaction ,Humans ,Sequence Analysis, DNA ,Disease Outbreaks ,Measles - Abstract
To ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005.Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain published in GenBank.4 measles viruses were isolated from 10 throat swab specimens, and the sequence analysis indicated that they belonged to H1 genotype. The homogeneity of 450 nucleotides in the C terminal of the N gene was at 98%-98.2% as compared to H1 genotype (China93-7). They differed from genotype H2 (China94-1) at 6.4%-6.9% and from genotype A (Edmonston) at 6.7%-6.9%, from measles vaccine (Shanghail91) at 7.6%-8.0%. They differed from the other measles viral strain isolated in China in 1993 - 2005 at 0.2%-3.7%. The variation within 4 isolated measles viruses was at 0.7%-1.3%.It was H1 genotype measles viruses,which are the native viruses in China that led to the outbreak of measles in Shanghai, in 2005.
- Published
- 2007
49. Numerical Simulation of Flows Over Underwater Axisymmetric Bodies With Full Appendages
- Author
-
Lian-Di Zhou and Zhen-Yu Huang
- Subjects
Curvilinear coordinates ,Engineering ,Computer simulation ,business.industry ,Numerical analysis ,Rotational symmetry ,Propeller ,Mechanics ,Wake ,Physics::Fluid Dynamics ,Classical mechanics ,Propulsor ,Underwater ,business - Abstract
Publisher Summary This chapter illustrates construction of a numerical method based on flux–difference splitting LU decomposition, implicit high-resolution third-order essentially nonoscillatory (ENO) schemes for efficient computations of steady-state solution to the three-dimensional, incompressible Navier–Stokes equations in curvilinear coordinates. The flow fields over underwater axisymmetric bodies with a fairwater and four stem appendages are also calculated. The third-order-accurate efficient ENO is applied in the calculations, which can capture the details of the flow field around underwater bodies with full appendages. The numerical results agree quite well with the experimental data. The numerical results show that the circumferential-mean nondimensional velocity profiles at propeller are in good agreement with the experimental data, which can be satisfied with the need of wake profile accuracy in the design of propuslor blade. The schemes and code developed in the chapter can be applied in the design of underwater vehicle propulsor and in the optimization and evaluation of its hydrodynamics noise. Also, the code can be used in the optimization and design of shapes of vehicle body and its appendages.
- Published
- 2001
50. Combining Geostatistics with Moran's I Analysis for Mapping Soil Heavy Metals in Beijing, China.
- Author
-
Xiao-Ni Huo, Hong Li, Dan-Feng Sun, Lian-Di Zhou, and Bao-Guo Li
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.