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Abstract Esophageal squamous cell carcinoma (ESCC) is a common fatal malignant tumor of the digestive tract; however, its pathogenic mechanism is unknown and lacks specific molecular diagnosis and treatment. Therefore, it is particularly important to identify new tumor biomarkers to enhance the early diagnosis and molecular-targeted therapy of ESCC. Here, we found that the E3 ubiquitin ligase Tripartitemotif-containing33 (TRIM33) is highly expressed in ESCC tissues and cell lines, and is associated with adverse clinical outcomes. We determined that TRIM33 drives aerobic glycolysis to promote tumor growth in vivo and in vitro. In terms of mechanism, TRIM33 binds to p53 to inhibit its stability and promote the expression of downstream glycolysis target genes GLUT1, HK2, PKM2, and LDHA. In addition, TRIM33 promotes the polyubiquitination of P53 K48-linked and proteasome degradation. Further studies have shown that the K351 site of P53 is the key site mediating the ubiquitination of P53 K48-linked to promote aerobic glycolysis in ESCC and tumor cell growth. Our results reveal that the TRIM33-P53 signal axis regulates glycolysis during ESCC and may provide a new perspective for the diagnosis and treatment of ESCC.

Abstract Background Cysticercosis is a zoonotic parasitic disease that poses a serious threat to public health. It is widely distributed and has a high incidence rate in China. Reports of disseminated cysticercosis worldwide are rare. This article presents a case of disseminated cysticercosis in the Guangxi Zhuang Autonomous Region of southwestern China. Case presentation The patient, a 46-year-old male belonging to the Miao ethnic group, hailed from a region in Guangxi Zhuang Autonomous Region known for its high incidence of cysticercosis. He had a habit of consuming raw pork and beef. With a history of recurrent consciousness disturbances and limb convulsions for five years, he presented with headaches and dizziness nine days prior. Comprehensive examinations were conducted on the patient. Ultimately, based on epidemiological history, imaging findings, pathogen testing, and pathological results, he was diagnosed with disseminated cysticercosis. Following anthelmintic treatment, the patient was discharged with clear consciousness, free from headaches, dizziness, nausea, vomiting, and seizures. The patient is currently under follow-up care. Conclusion It is crucial to enhance public awareness, promote health education, and cultivate good hygiene habits, as these are essential measures in reducing the incidence of cysticercosis.

Abstract Background Alveolar soft part sarcoma (ASPS) occurs most often in the deep muscles or fascia of the extremities in adults, with only 3.4% of these tumours originating from the head, face and neck. To date, only 17 cases of buccal ASPS have been reported, including the case presented here. Only one case of ASPS recurrence at the primary site, similar to our case, has been reported thus far. Immune checkpoint inhibitors (ICPis)-associated diabetes, with an estimated incidence of 0.43%, is usually seen in older cancer patients and has not been reported in younger people or in patients with ASPS. Case presentation A 24-year-old male patient presented with a slowly progressing right cheek mass with a clinical history of approximately 28 months. Sonographic imaging revealed a hypoechoic mass, which was considered a benign tumour. However, a pathological diagnosis of ASPS was made after excision of the mass. Five days later, functional right cervical lymph node dissection was performed. No other adjuvant therapy was administered after surgery. In a periodic follow-up of the patient six months later, blood-rich tumour growth was noted at the primary site, and Positron emission tomography-computedtomography (PET-CT) ruled out distant metastasis in other areas. The patient was referred to the Ninth People’s Hospital of Shanghai Jiaotong University. Due to the large extent of the mass, the patient received a combination of a Programmed Cell Death Ligand 1(PD-L1) inhibitor and a targeted drug. Unfortunately, the patient developed three episodes of severe diabetic ketoacidosis after the administration of the drugs. A confirmed diagnosis of ICPis-associated diabetes was confirmed. After the second operation, the postoperative pathological diagnosis was ASPS, and the margins were all negative. Therefore, we made a final clinical diagnosis of ASPS recurrence at the primary site. Currently in the follow-up, the patient is alive, has no distant metastases, and undergoes multiple imaging examinations every 3 months for the monitoring of their condition. Conclusions In analysing the characteristics of all previously reported cases of buccal ASPS, it was found that the clinical history ranged from 1 to 24 months, with a mean of approximately 3 to 9 months. Tumour recurrence at the primary site has been reported in only one patient with buccal ASPS, and the short-term recurrence in our patient may be related to the extraordinarily long 28-month history. ICPis-associated diabetes may be noted in young patients with rare tumours, and regular insulin level monitoring after use is necessary.

Autoimmune glial fibrillary acidic protein (GFAP) astrocytopathy is a rare autoimmune disease, which is characterized by the immune system attacking astrocytes in the central nervous system, resulting in inflammation and damage to the nervous system. We reported a 41-year-old female patient with only drowsiness for 3 months, who was, otherwise, healthy with no other signs of meningoencephalitis or myelitis. There were no obvious abnormalities in her neurological and ophthalmic tests. Brain magnetic resonance imaging (MRI) plain scan + enhancement with the gadolinium contrast agent revealed patchy hypointensity on T1-weighted imaging, hyperintensity on T2-weighted imaging, hyperintensity on T2-weighted fluid-attenuated inversion recovery in the left basal ganglia, corona radiata, and local septum pellucida, with no enhancement in the enhanced lesions. Cerebrospinal fluid (CSF) revealed white blood cell count of 5.00 × 106/L, CSF protein of 828.53 mg/L, and glucose of 2.83 mmol/L. Aquaporin-4 (AQP4) antibody, N-methyl-D-aspartate receptor (NMDAR) antibody and GFAP antibody were all positive, whereas the remaining autoimmune encephalitis antibody tests were negative. Oncology screening [including head, chest, and whole-abdomen (involving the pelvic cavity) CT and tumor markers] did not reveal any obvious evidence of tumor presence. The patient received systemic treatment with high-dose intravenous injection of steroids combined with immunosuppressive agents, and the clinical and imaging features of the patients improved. To the best of our knowledge, reports on overlapping positivity of AQP4 antibody and NMDAR antibody in patients with GFAP astrocytopathy were still very rare. We hope to supplement the existing literature on this topic, review the relevant literature, and strive to increase the understanding toward GFAP astrocytopathy with overlapping autoimmune syndrome so as to enable early diagnosis and early treatment and to improve the clinical outcome of patients.

【Objective】Natural disasters such as earthquakes have the characteristics of persistence and wide range. During the occurrence of disasters, the link resources of the optical network will be continuously damaged, resulting in the constant change of the link risk. In the face of constantly changing link risks, improper service recovery planning may cause service failures. From a service perspective, repeated faults will interrupt data transmission many times, and the subsequent link status damage may be aggravated after a disaster occurs. From the perspective of network management and control, repeated recovery wastes route calculation resources and occupies the recovery resources of other services. At the same time, different services have different requirements for transmission reliability because of the importance of the data to be transmitted. When a fault occurs, the high-importance services should be recovered first. Therefore, in the scenario of a large-scale persistent disaster, it is a problem worth studying to comprehensively consider the sustained impact of the disaster on link risk and the difference in path reliability requirements of different services for service recovery. To solve this problem, this paper proposes a link risk-aware service recovery algorithm-Dynamic Link Risk Reroute Algorithm (DLRRA) under persistent disasters.【Methods】Firstly, according to the service importance and link risk, we establish the service importance evaluation model and link risk evaluation model. Then we propose the optimization target route reliability. The DLRRA, combined with the optimization objective, fully considers the change of link risk degree caused by the impact of disasters on the continuity of links. By preferentially allocating low-risk recovery resources to the fault services of high importance, the risk of secondary failure of the same high-importance services is avoided during the continuous occurrence of disasters.【Results】The simulation results show that the second failure probability of DLRRA recovery is reduced by 11% compared with the traditional algorithm, and the average importance of DLRRA recovery under the high load is increased by 10%.【Conclusion】Therefore, the algorithm effectively avoids the loss caused by multiple service interruptions caused, and ensures the continuous and stable operation of important services in the disaster environment.

Abstract Background GEFT is a key regulator of tumorigenesis in rhabdomyosarcoma (RMS), and overexpression of GEFT is significantly correlated with distant metastasis, lymph node metastasis, and a poor prognosis, yet the underlying molecular mechanism is still poorly understood. This study aimed to investigate and validate the molecular mechanism of GEFT-activated lncRNAs in regulating mTOR expression to promote the progression of RMS. Methods GEFT-regulated lncRNAs were identified through microarray analysis. The effects of GEFT-regulated lncRNAs on the proliferation, apoptosis, invasion, and migration of RMS cells were confirmed through cell functional experiments. The target miRNAs of GEFT-activated lncRNAs in the regulation of mTOR expression were predicted by bioinformatics analysis combined with quantitative real-time polymerase chain reaction (qRT–PCR) analysis. The expression of lnc-PSMA8-1, miR-144-3p, and mTOR was measured by qRT–PCR in RMS tissue samples and cell lines. The regulatory mechanisms of the lnc-PSMA8-1-miR-144-3p-mTOR signaling axis were verified by RNA-binding protein immunoprecipitation (RIP), a luciferase reporter assay, qRT–PCR analysis, Western blot analysis, and cell functional experiments. Results The microarray-based analysis identified 31 differentially expressed lncRNAs (fold change > 2.0, P

For the coil misalignment problem in the charging process of automatic guidance vehicles (AGVs), this paper proposes an optimization method for the bilateral LCC compensation network parameters based on quadruple-D quadrature (4DQ) coils. By introducing two coefficients $K_{\text {T}}$ and $K_{\text {f1}}$ , the compensation capacitors $C_{\text {T}}$ and $C_{\text {f1}}$ are optimized, achieving smaller output power fluctuations. Firstly, the capacitor values $C_{\text {T}}$ and $C_{\text {f1}}$ at resonance in the SS and PS equivalent circuit topology are obtained, meanwhile, the relationship between the output power $P_{\text {out}}$ and the coupling coefficient $k$ , represented by the coefficients $K_{\text {T}}$ and $K_{\text {f1}}$ , is obtained. Furthermore, with the transmission efficiency as the limiting condition, the output power fluctuation is effectively suppressed by adjusting $K_{\text {T}}$ and $K_{\text {f1}}$ . Finally, the experimental results show that after optimization the output power is 427.08W, and the transmission efficiency is 93.506% when the coupling coil are aligned, the system can still maintain a power output of at least 382.36W with a coil offset of 0-153mm in the X/Y axis directions, and the transmission efficiency is always above 89.472%. At the same time, the maximum deviation value $\Delta P_{{\text {abs}\_{}\text{max}}}$ decreased from 213.1W before optimization to 111.0W after optimization, a decrease of 47.91%, effectively suppressing the power fluctuation and improving anti-offset performance.

ObjectiveThis meta-analysis aims to assess the effectiveness and safety of robot-assisted deep brain stimulation (DBS) surgery for Parkinson’s disease(PD).MethodsFour databases (Medline, Embase, Web of Science and CENTRAL) were searched from establishment of database to 23 March 2024, for articles studying robot-assisted DBS in patients diagnosed with PD. Meta-analyses of vector error, complication rate, levodopa-equivalent daily dose (LEDD), Unified Parkinson’s Disease Rating Scale (UPDRS), UPDRS II, UPDRS III, and UPDRS IV were performed.ResultsA total of 15 studies were included in this meta-analysis, comprising 732 patients with PD who received robot-assisted DBS. The pooled results revealed that the vector error was measured at 1.09 mm (95% CI: 0.87 to 1.30) in patients with Parkinson’s disease who received robot-assisted DBS. The complication rate was 0.12 (95% CI, 0.03 to 0.24). The reduction in LEDD was 422.31 mg (95% CI: 68.69 to 775.94). The improvement in UPDRS, UPDRS III, and UPDRS IV was 27.36 (95% CI: 8.57 to 46.15), 14.09 (95% CI: 4.67 to 23.52), and 3.54 (95% CI: −2.35 to 9.43), respectively.ConclusionRobot-assisted DBS is a reliable and safe approach for treating PD. Robot-assisted DBS provides enhanced accuracy in contrast to conventional frame-based stereotactic techniques. Nevertheless, further investigation is necessary to validate the advantages of robot-assisted DBS in terms of enhancing motor function and decreasing the need for antiparkinsonian medications, in comparison to traditional frame-based stereotactic techniques.Clinical trial registration: PROSPERO(CRD42024529976).

Xenotransplantation, involving animal organ transplantation into humans to address the human organ shortage, has been studied since the 17th century. Early attempts to obtain organs from animals such as goats, dogs, and non-human primates proved unsuccessful. In the 1990s, scientists agreed that pigs were the most suitable donor animals for xenotransplantation. However, immune rejection between pig and human has hindered the application. To overcome these challenges, researchers developed genetically modified pigs that deactivate xenoreactive antigen genes and express human protective genes. These advances extended xenograft survival from days to years in non-human primates, resulting in the first human heart xenotransplant trial. Using genetically engineered pigs for the organ shortage is promising. This review provides an overview of potential incompatibilities of immunogenicity and functional proteins related to xenotransplantation between humans and pigs. Furthermore, it elucidates possible approaches for multiplex gene modification to breed better-humanized pigs for clinical xenotransplantation.

Objective To investigate the effects of preoperative pre-rehabilitation on postoperative gastrointestinal function, nutritional indicators, quality of life and adverse reactions in patients with gastric cancer. Methods A total of 66 patients who underwent radical gastrectomy for gastric cancer in the 901st Hospital of Joint Logistics Support Force of the CPLA from November 2019 to March 2022 were selected as the research subjects. The patients were divided into control group (n=35) and study group (n=31) by random number table method. The control group received routine preoperative preparation, and the study group received preoperative pre-rehabilitation. Postoperative gastrointestinal function recovery (first postoperative exhaust time, defecation time and the time to first oral feeding), serum nutritional indicators ［total protein (TP), albumin (Alb), prealbumin (PA) and hemoglobin (Hb)］, postoperative gastrointestinal adverse reactions during enteral nutrition, and the generic quality of life inventory-74 (GQOL-74) scores were compared between two groups. Results The time to first oral feeding, defecation recovery time and exhaust time in the study group after operation were significantly shorter than those in the control group (P

Abstract Objective The incidence of open reduction and internal fixation (ORIF) in flexion-type supracondylar humerus fractures (SCHF) in children is significantly higher than that of extension-type fractures. This study aimed to identify risk factors for ORIF in flexion-type SCHF. Methods One hundred seventy-one patients with Wilkins type III flexion-type SCHF from January 2012 to December 2021 were retrospectively enrolled in a tertiary paediatric hospital. Patients were divided into ORIF group versus closed reduction and internal fixation (CRIF) group. Then, patients data of age, sex, injury side, obesity, deviation of displacement, fracture level, rotation, nerve injury, and delay from injury to surgery were reviewed. Univariate analysis and multivariate logistic regression were used to identify independent risk factors and odds ratios (OR) of ORIF. Results Overall, 171 children with type III flexion-type SCHF were analyzed (average aged 7.9 ± 2.8 years). Displacement was lateral in 151 cases, medial in 20. 20 cases had combined ulnar nerve injury. The failed closed reduction rate was 20%. Univariate analysis indicated age, distal fracture fragment rotation, and ulnar nerve injury were significantly associated with ORIF. (P = 0.047, P = 0.009, and P = 0.001, respectively). Multivariate logistic regression analysis showed that distal fracture fragment rotation (OR, 3.3; 95%CI:1.1–9.5; P = 0.028) and ulnar nerve injury (OR, 6.4; 95%CI:2.3–18.3; P = 0.001) were independent risk factors; however, the age was not an independent one (OR, 1.5; 95%CI:0.6–3.5; P = 0.397) for ORIF in the Wilkins type III flexion-type SCHF. Conclusion Distal fracture fragment malrotation on initial x-rays and ulnar nerve injury were significant risk factors for ORIF in Wilkins type III flexion-type SCHF. Surgeons should prepare tourniquets or other open reduction instruments when treating these types of fractures. Level of evidence Level IV

Glycosylase base editor (GBE) can induce C‐to‐G transversion in mammalian cells, showing great promise for the treatment of human genetic disorders. However, the limited efficiency of transversion and the possibility of off‐target effects caused by Cas9 restrict its potential clinical applications. In our recent study, we have successfully developed TaC9‐CBE and TaC9‐ABE by separating nCas9 and deaminase, which eliminates the Cas9‐dependent DNA off‐target effects without compromising editing efficiency. We developed a novel GBE called TaC9‐GBEYE1, which utilizes the deaminase and UNG‐nCas9 guided by TALE and sgRNA, respectively. TaC9‐GBEYE1 showed comparable levels of on‐target editing efficiency to traditional GBE at 19 target sites, without any off‐target effects caused by Cas9 or TALE. The TaC9‐GBEYE1 is a safe tool for gene therapy. [ABSTRACT FROM AUTHOR]

Diplazium zangnanense, a new species of Diplazium Sw. from Medog county, Xizang Autonomous Region, China, is described and illustrated. Morphological comparisons between this new species and similar ones (e.g. D. kawakamii, D. medogense, D. muricatum, D. multicaudatum and D. spectabile) are provided. Molecular evidence shows that the new species is closely related to D. multicaudatum. The most distinctive characters of D. zangnanense are its decumbent to erect rhizomes, bicolorous scales with toothed margin on the stems, and oblong sori. [ABSTRACT FROM AUTHOR]

This paper investigates the mechanical properties of a traditional welded rigid joint with a weakened panel zone under seismic load. The created finite element model is calibrated by the high-strength steel joint test, carried out by the team in the early stage, and the effectiveness of the finite element method was verified. The finite element software ABAQUS is used to investigate the influence of different joint web thicknesses on the mechanical properties of middle column joints under a low-cyclic-loading test. Supported by a validated numerical model, the ductility, energy dissipation, and other properties of different thicknesses of panel zone column webs are carefully analyzed. The results indicate that the thickness of the web plate in the panel zone significantly affects the location of the joint plastic hinge. The ultimate loading capacity of the joints increased significantly with an increase in the thickness of the webs in the panel zones. Compared with the joint with a weakened panel zone, the hysteresis curve of the strengthened joint is fuller; meanwhile, it cannot alleviate the stress concentration at the weld holes of the web. When the thickness of the joint domain web is too weak, excessive deformation in the joint domain will lead to a decrease in the bearing capacity of the joint, causing damage. The stiffness degradation coefficient of the web-thickened specimen was found to be dominated and controlled by the stiffness of the beam; however, with an increase in the thickness of the web, the stiffness degradation coefficient remained basically unchanged. Finally, a recommendation for weakened beam–column interior joints based on the steel frame panel zone is made, which will lay a foundation for the simulation and analysis of the seismic performance of this structure.

This study proposed a framework of optimal design for flexible cable dome structures based on progressive collapse resistance. First, a quantitative evaluation method for nonlinear robustness based on robustness control theory to reflect the structural progressive collapse resistance was proposed. Second, an actual engineering structure was used as a case study to evaluate the effects of design parameters on structural robustness. Finally, a genetic algorithm was used as an optimisation algorithm to further optimise the element cross-section and the structural shape and obtain a combined optimisation rate. The results indicated that increasing the element cross-sectional area, decreasing the structural span, and increasing the rise-to-span ratio effectively improved the structural robustness. The structural robustness was also effectively improved through the optimal design of element cross-sections by increasing element cross-sections sensitive to structural robustness and decreasing those insensitive to structural robustness. In this study, the combined optimisation rate was 38.27%, which was not only greater than the individual optimisation rates of 11.2% for element cross-sectional area optimisation and 22.5% for structural shape optimisation but also the sum of these two rates.