183 results on '"Li-ping Zhou"'
Search Results
2. Blockade of adenosine A2A receptors reverses early spatial memory defects in the APP/PS1 mouse model of Alzheimer’s disease by promoting synaptic plasticity of adult-born granule cells
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Qi Ji, Yang Yang, Yun Xiong, Ying-Jie Zhang, Jun Jiang, Li-Ping Zhou, Xiao-Hui Du, Chun-Xiang Wang, and Zhi-Ru Zhu
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Alzheimer’s disease ,Spatial memory ,Adenosine A2A receptors ,LTP ,Adult born granule cells ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background The over-activation of adenosine A2A receptors (A2AR) is closely implicated in cognitive impairments of Alzheimer's disease (AD). Growing evidence shows that A2AR blockade possesses neuroprotective effects on AD. Spatial navigation impairment is an early manifestation of cognitive deficits in AD. However, whether A2AR blockade can prevent early impairments in spatial cognitive function and the underlying mechanism is still unclear. Methods A transgenic APP/PS1 mouse model of AD amyloidosis was used in this study. Behavioral tests were conducted to observe the protective effects of A2AR blockade on early spatial memory deficits in 4-month old APP/PS1 mice. To investigate the underlying synaptic mechanism of the protective effects of A2AR blockade, we further examined long-term potentiation (LTP) and network excitation/inhibition balance of dentate gyrus (DG) region, which is relevant to unique synaptic functions of immature adult-born granule cells (abGCs). Subsequently, the protective effects of A2AR blockade on dendritic morphology and synaptic plasticity of 6-week-old abGCs was investigated using retrovirus infection and electrophysiological recordings. The molecular mechanisms underlying neuroprotective properties of A2AR blockade on the synaptic plasticity of abGCs were further explored using molecular biology methods. Results APP/PS1 mice displayed DG-dependent spatial memory deficits at an early stage. Additionally, impaired LTP and an imbalance in network excitation/inhibition were observed in the DG region of APP/PS1 mice, indicating synaptic structural and functional abnormalities of abGCs. A2AR was found to be upregulated in the hippocampus of the APP/PS1 mouse model of AD. Treatment with the selective A2AR antagonist SCH58261 for three weeks significantly ameliorated spatial memory deficits in APP/PS1 mice and markedly restored LTP and network excitation/inhibition balance in the DG region. Moreover, SCH58261 treatment restored dendritic morphology complexity and enhanced synaptic plasticity of abGCs in APP/PS1 mice. Furthermore, SCH58261 treatment alleviated the impairment of synaptic plasticity in abGCs. It achieved this by remodeling the subunit composition of NMDA receptors and increasing the proportion of NR2B receptors in abGCs of APP/PS1 mice. Conclusions Blockade of A2AR improves early spatial memory deficits in APP/PS1 mice, possibly by reversing synaptic defects of abGCs. This finding suggests that A2AR blockade could be a potential therapy for AD.
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- 2023
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3. Combining nitrogen effects and metabolomics to reveal the response mechanisms to nitrogen stress and the potential for nitrogen reduction in maize
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Yan-li LU, Gui-pei SONG, Yu-hong WANG, Luo-bin WANG, Meng-ze XU, Li-ping ZHOU, and Lei WANG
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long-term experiment ,nitrogen deficiency ,nitrogen excess ,metabolites ,UPLC-QTOF ,Agriculture (General) ,S1-972 - Abstract
The physiological and metabolic differences in maize under different nitrogen (N) levels are the basis of reasonable N management, which is vital in improving fertilizer utilization and reducing environmental pollution. In this paper, on the premise of defining the N fertilizer efficiency and yield under different long-term N fertilization treatments, the corresponding differential metabolites and their metabolic pathways were analyzed by untargeted metabolomics in maize. N stress, including deficiency and excess, affects the balance of carbon (C) metabolism and N metabolism by regulating C metabolites (sugar alcohols and tricarboxylic acid (TCA) cycle intermediates) and N metabolites (various amino acids and their derivatives). L-alanine, L-phenylalanine, L-histidine, and L-glutamine decreased under N deficiency, and L-valine, proline, and L-histidine increased under N excess. In addition to sugar alcohols and the above amino acids in C and N metabolism, differential secondary metabolites, flavonoids (e.g., kaempferol, luteolin, rutin, and diosmetin), and hormones (e.g., indoleacetic acid, trans-zeatin, and jasmonic acid) were initially considered as indicators for N stress diagnosis under this experimental conditions. This study also indicated that the leaf metabolic levels of N2 (120 kg ha–1 N) and N3 (180 kg ha–1 N) were similar, consistent with the differences in their physiological indexes and yields over 12 years. This study verified the feasibility of reducing N fertilization from 180 kg ha–1 (locally recommended) to 120 kg ha–1 at the metabolic level, which provided a mechanistic basis for reducing N fertilization without reducing yield, further improving the N utilization rate and protecting the ecological environment.
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- 2023
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4. Combination of alpha-fetoprotein and neutrophil-to-lymphocyte ratio to predict treatment response and survival outcomes of patients with unresectable hepatocellular carcinoma treated with immune checkpoint inhibitors
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Hong-Fei Zhu, Jin-Kai Feng, Yan-Jun Xiang, Kang Wang, Li-Ping Zhou, Zong-Han Liu, Yu-Qiang Cheng, Jie Shi, Wei-Xing Guo, and Shu-Qun Cheng
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Hepatocellular carcinoma (HCC) ,Immune checkpoint inhibitor (ICI) ,Immunotherapy ,Predictive score ,Overall survival (OS) ,Progression-free survival (PFS) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Immune-checkpoint inhibitors (ICIs) have revolutionized the treatment of hepatocellular carcinoma (HCC). However, long-term survival outcomes and treatment response of HCC patients undergoing immunotherapy is unpredictable. The study aimed to evaluate the role of alpha-fetoprotein (AFP) combined with neutrophil-to-lymphocyte ratio (NLR) to predict the prognosis and treatment response of HCC patients receiving ICIs. Methods Patients with unresectable HCC who received ICI treatment were included. The HCC immunotherapy score was developed from a retrospective cohort at the Eastern Hepatobiliary Surgery Hospital to form the training cohort. The clinical variables independently associated with overall survival (OS) were identified using univariate and multivariate Cox regression analysis. Based on multivariate analysis of OS, a predictive score based on AFP and NLR was constructed, and patients were stratified into three risk groups according to this score. The clinical utility of this score to predict progression-free survival (PFS) and differentiate objective response rate (ORR) and disease control rate (DCR) was also performed. This score was validated in an independent external validation cohort at the First Affiliated Hospital of Wenzhou Medical University. Results Baseline AFP ≤ 400 ng/ml (hazard ratio [HR] 0.48; 95% CI, 0.24–0.97; P = 0.039) and NLR ≤ 2.77 (HR 0.11; 95% CI, 0.03–0.37; P 400 ng/ml and 3 points for NLR > 2.77. Patients with 0 point were classified as the low-risk group. Patients with 1–3 points were categorized as the intermediate-risk group. Patients with 4 points were classified as the high-risk group. In the training cohort, the median OS of the low-risk group was not reached. The median OS of the intermediate-risk group and high-risk group were 29.0 (95% CI 20.8–37.3) months and 16.0 (95% CI 10.8–21.2) months, respectively (P
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- 2023
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5. Efficacy and safety of TACE combined with lenvatinib and PD‐1 inhibitors for unresectable recurrent HCC: A multicenter, retrospective study
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Wei‐Jun Wang, Zong‐Han Liu, Kang Wang, Hong‐Ming Yu, Yu‐Qiang Cheng, Yan‐Jun Xiang, Jin‐Kai Feng, Li‐Ping Zhou, Hong‐Kun Zhou, Wei‐Wei Pan, Wei‐Xing Guo, Jie Shi, and Shu‐Qun Cheng
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combination therapy ,hepatocellular carcinoma (HCC) ,lenvatinib ,programmed death‐1 ,recurrent ,transarterial chemoembolization (TACE) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background There is no consensus on the optimal regimen for unresectable recurrent hepatocellular carcinoma (HCC), so this retrospective study aimed to evaluate the efficacy and safety of transarterial chemoembolization (TACE) combined with lenvatinib and PD‐1 inhibitors (T‐L‐P) versus TACE combined with lenvatinib (T‐L) or TACE alone. Method Data were collected from 204 patients with unresectable recurrent HCC who received T‐L‐P, T‐L, or TACE alone at three medical centers from January, 2019 to December, 2020 for analysis. The survival outcomes, tumor response, and adverse events were compared between three groups, and risk factors were further investigated. Results The median overall survival in the T‐L‐P, T‐L, and TACE alone groups were not reached, 25.6, and 15.7 months, respectively (p
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- 2023
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6. Efficacy and safety of a triple combination of atezolizumab, bevacizumab plus GEMOX for advanced biliary tract cancer: a multicenter, single-arm, retrospective study
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Kang Wang, Zong-Han Liu, Hong-Ming Yu, Yu-Qiang Cheng, Yan-Jun Xiang, Jing-Ya Zhong, Qian-Zhi Ni, Li-Ping Zhou, Chao Liang, Hong-Kun Zhou, Wei-Wei Pan, Wei-Xing Guo, Jie Shi, and Shu-Qun Cheng
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background: Anti-programmed cell death ligand 1/vascular endothelial growth factor inhibition, coupled with chemotherapy, may potentiate antitumor immunity leading to enhanced clinical benefit, but it has not been investigated in advanced biliary tract cancer (BTC). Objectives: We investigated the efficacy and safety of atezolizumab, bevacizumab, and gemcitabine plus oxaliplatin (GEMOX) in advanced BTC and explore the potential biomarkers related to the response. Design: Multicenter, single-arm, retrospective study. Methods: Advanced BTC patients, who received a triple combination therapy at three medical centers between 18 March 2020 and 1 September 2021, were included. Treatment response was evaluated via mRECIST and RECIST v1.1. Endpoints included the overall response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS), and safety. The whole exome sequencing of pathological tissues was conducted for bioinformatic analysis. Results: In all, 30 patients were enrolled. The best ORR was 76.7% and the DCR was 90.0%. The median PFS was 12.0 months, and the median OS was not reached. During the treatment, 10.0% (3/30) of patients suffered from ⩾grade 3 treatment-related adverse events (TRAEs). Furthermore, fever (73.3%), neutropenia (63.3%), increased aspartate transaminase and alanine aminotransferase levels (50.0% and 43.3%, respectively) are the most common TRAEs. Bioinformatics analysis revealed patients with altered ALS2CL had a higher ORR. Conclusion: The triple combination of atezolizumab, bevacizumab, and GEMOX may be efficacious and safe for patients with advanced BTC. ALS2CL may be a potential predictive biomarker for the efficacy of triple combination therapy.
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- 2023
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7. Polydopamine-modified chitin conduits with sustained release of bioactive peptides enhance peripheral nerve regeneration in rats
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Ci Li, Song-Yang Liu, Li-Ping Zhou, Tian-Tian Min, Meng Zhang, Wei Pi, Yong-Qiang Wen, and Pei-Xun Zhang
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angiogenesis ,bioactive peptides ,nerve repair ,neurotrophic factor ,peripheral nerve injury ,peripheral nerve regeneration ,polydopamine ,surface modification ,synergistic effects ,tissue engineering ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
[INLINE:1] The introduction of neurotrophic factors into injured peripheral nerve sites is beneficial to peripheral nerve regeneration. However, neurotrophic factors are rapidly degraded in vivo and obstruct axonal regeneration when used at a supraphysiological dose, which limits their clinical benefits. Bioactive mimetic peptides have been developed to be used in place of neurotrophic factors because they have a similar mode of action to the original growth factors and can activate the equivalent receptors but have simplified sequences and structures. In this study, we created polydopamine-modified chitin conduits loaded with brain-derived neurotrophic factor mimetic peptides and vascular endothelial growth factor mimetic peptides (Chi/PDA-Ps). We found that the Chi/PDA-Ps conduits were less cytotoxic in vitro than chitin conduits alone and provided sustained release of functional peptides. In this study, we evaluated the biocompatibility of the Chi/PDA-Ps conduits. Brain-derived neurotrophic factor mimetic peptide and vascular endothelial growth factor mimetic peptide synergistically promoted proliferation of Schwann cells and secretion of neurotrophic factors by Schwann cells and attachment and migration of endothelial cells in vitro. The Chi/PDA-Ps conduits were used to bridge a 2 mm gap between the nerve stumps in rat models of sciatic nerve injury. We found that the application of Chi/PDA-Ps conduits could improve the motor function of rats and reduce gastrocnemius atrophy. The electrophysiological results and the microstructure of regenerative nerves showed that the nerve conduction function and remyelination was further restored. These findings suggest that the Chi/PDA-Ps conduits have great potential in peripheral nerve injury repair.
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- 2022
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8. Efficacy and safety of transarterial chemoembolization plus antiangiogenic- targeted therapy and immune checkpoint inhibitors for unresectable hepatocellular carcinoma with portal vein tumor thrombus in the real world
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Jin-Kai Feng, Zong-Han Liu, Zhi-Gang Fu, Zong-Tao Chai, Ju-Xian Sun, Kang Wang, Yu-Qiang Cheng, Hong-Fei Zhu, Yan-Jun Xiang, Li-Ping Zhou, Jie Shi, Wei-Xing Guo, Jian Zhai, and Shu-Qun Cheng
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hepatocellular carcinoma (HCC) ,portal vein tumor thrombus (PVTT) ,transarterial chemoembolization (TACE) ,anti-angiogenic targeted therapy ,PD-1 inhibitor ,immune checkpoint inhibitor (ICI) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
PurposeThis study aimed to assess the efficacy and safety of a triple therapy that comprises transarterial chemoembolization (TACE), antiangiogenic-targeted therapy, and programmed death-1 (PD-1) inhibitors in a real-world cohort of patients with unresectable hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT).MethodsConsecutive patients treated with TACE combined with antiangiogenic therapy and PD-1 inhibitors at the Eastern Hepatobiliary Surgery Hospital between June 2019 and May 2021 were enrolled. The baseline characteristics and treatment course of the patients were recorded. The tumor response was evaluated based on the Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 and HCC-specific modified RECIST (mRECIST). The overall survival (OS) and progression-free survival (PFS) of the patients were analyzed using the Kaplan–Meier method. Adverse events (AEs) were assessed according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0.ResultsAs of the data cutoff on 30 August 2021, the median follow-up time was 10.0 (3.9–28.4) months. A total of 39 eligible patients were included. The objective response rate (ORR) and the disease control rate (DCR) were 35.9% and 74.4% according to the RECIST 1.1, and 48.7% and 84.6% according to mRECIST criteria, respectively. The median OS and PFS were 14.0 and 9.2 months, respectively. Moreover, 34 (87.2%) patients experienced at least one treatment-related AE and 8 (20.5%) patients experienced grade 3/4 treatment-related AEs. The most common treatment- and laboratory-related AEs were hypertension (46.2%) and decreased albumin (53.8%), respectively. No treatment-related mortality occurred during the study period.ConclusionsTACE combined with antiangiogenic-targeted therapy and immune checkpoint inhibitors may have promising anticancer activity in unresectable HCC patients with PVTT. AEs were manageable, with no unexpected overlapping toxicities.
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- 2022
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9. Identification of the Microbial Transformation Products of Secoisolariciresinol Using an Untargeted Metabolomics Approach and Evaluation of the Osteogenic Activities of the Metabolites
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Wen-Xuan Yu, Hok-Him Tang, Jun-Jie Ye, Hui-Hui Xiao, Chung-Yan Lam, Tim-Fat Shum, Zhi-Kang Sun, Yuan-Zhen Li, Xin-Yu Zang, Wen-Chao Du, Jian-Ping Zhang, Tsz-Hung Kong, Li-Ping Zhou, Jia-Chi Chiou, Chun-Fai Kung, Kam-Wah Mok, Jing Hu, and Man-Sau Wong
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secoisolariciresinol ,microbial transformation ,metabolites ,osteogenic activities ,Organic chemistry ,QD241-441 - Abstract
Secoisolariciresinol (SECO) is one of the major lignans occurring in various grains, seeds, fruits, and vegetables. The gut microbiota plays an important role in the biotransformation of dietary lignans into enterolignans, which might exhibit more potent bioactivities than the precursor lignans. This study aimed to identify, synthesize, and evaluate the microbial metabolites of SECO and to develop efficient lead compounds from the metabolites for the treatment of osteoporosis. SECO was fermented with human gut microbiota in anaerobic or micro-aerobic environments at different time points. Samples derived from microbial transformation were analyzed using an untargeted metabolomics approach for metabolite identification. Nine metabolites were identified and synthesized. Their effects on cell viability, osteoblastic differentiation, and gene expression were examined. The results showed that five of the microbial metabolites exerted potential osteogenic effects similar to those of SECO or better. The results suggested that the enterolignans might account for the osteoporotic effects of SECO in vivo. Thus, the presence of the gut microbiota could offer a good way to form diverse enterolignans with bone-protective effects. The current study improves our understanding of the microbial transformation products of SECO and provides new approaches for new candidate identification in the treatment of osteoporosis.
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- 2023
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10. Cerebral Infarction as the Primary Presentation of Acute Aortic Dissection
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Li-Ping Zhou, Xiang-Min Li, Guo-Qing Huang, and Fang-Jie Zhang
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aortic dissection ,cerebral infarction ,missed diagnosis ,initial manifestation ,emergency ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background: The aim of this study was to determine the clinical characteristics and outcome of patients with aortic dissection (AD) who present with an initial manifestation of cerebral infarction. Methods: We retrospectively analyzed patients who were diagnosed with AD and admitted to the emergency department from May 1, 2017 to May 1, 2022. Data was collected for variables including age, sex, clinical manifestation, past medical history, and laboratory test results. Results: Twenty-five patients (2.61%, 22 type A and 3 type B) showed cerebral infarction as the primary presentation for acute AD, while another 933 AD patients (471 type A and 462 type B) who presented with other symptoms served as the control group. Eighteen of the 25 patients (72%) were initially diagnosed with stroke, and the diagnosis of AD was missed. However, patients with a missed diagnosis of AD did not have significantly different mortality to those in whom AD was diagnosed (chi-square test, p > 0.9999). Patients with cerebral infarction as the first presentation had a higher incidence of type A AD than the control patients (p = 0.0002), while their mortality rate was also higher than the control group of AD patients (p < 0.0001). Furthermore, patients with cerebral infarction as the first presentation were more likely to have multiple organ dysfunction. Conclusions: AD with an initial presentation of cerebral infarction is a rare condition with high mortality. However, the initial failure to diagnose AD does not further increase patient mortality.
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- 2023
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11. Clinical Features of Aortic Dissection in the Emergency Department: A Single-center Experience from South China
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Xiang-Min Li, Guo-Qing Huang, Ai-min Wang, Li-Ping Zhou, Xiao-Ye Mo, and Fang-Jie Zhang
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Medicine ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Objectives: Our goal in this study was to determine 1) whether there are any differences in clinical characteristics between Chinese and Western patients with aortic dissection (AD), and 2) the mortality rate of AD patients in the emergency department (ED) and identify the risk predictors for death. Methods: We retrospectively analyzed patients who were diagnosed with AD and admitted to our ED between September 1, 2017–August 31, 2020. Data on age, gender, clinical manifestation, medical history, routine blood tests, liver and kidney function, coagulation, myocardial enzymology, and mortality were collected. Results: We enrolled 535 AD patients (422 men and 113 women) with a mean age of 54.7±14.1 years. Type A AD constituted 40% of the total number of AD cases, while type B AD constituted 60%. The proportion of those who were females, 10–92 years, with type A AD, and hypertension in the Chinese population was lower than that in the Western population (P
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- 2022
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12. Effects of Stereotactic Body Radiation Therapy Plus PD-1 Inhibitors for Patients With Transarterial Chemoembolization Refractory
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Yan-Jun Xiang, Kang Wang, Yi-Tao Zheng, Shuang Feng, Hong-Ming Yu, Xiao-Wei Li, Xi Cheng, Yu-Qiang Cheng, Jin-Kai Feng, Li-Ping Zhou, Yan Meng, Jian Zhai, Yun-Feng Shan, and Shu-Qun Cheng
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hepatocellular carcinoma ,stereotactic body radiation therapy ,transarterial chemoembolization refractory ,immunotherapy ,combination therapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and AimsPatients with intermediate-stage hepatocellular carcinoma (HCC) who are refractory to transarterial chemoembolization (TACE) have a poor prognosis. This study aimed to explore whether stereotactic body radiation therapy (SBRT) combined with PD-1 inhibitors could improve the clinical outcomes of such patients.MethodsThis retrospective cohort study included patients with intermediate-stage HCC who were diagnosed with TACE refractoriness between January 2019 and December 2020 in the Eastern Hepatobiliary Surgery Hospital and the First Affiliated Hospital of Wenzhou Medical University. The patients were divided into two groups: (1) those who switched from TACE to receive stereotactic body radiotherapy (SBRT) combined with PD-1 inhibitors; (2) those who continued TACE treatment and added PD-1 inhibitors. Progression-free survival (PFS), overall survival (OS), and tumour response were assessed in both groups after becoming refractory to TACE treatment.ResultsOf the seventy-six patients included in this study, the median PFS was 19.6 months in the SBRT-IO group (n=31) and 10.1 months in the TACE-IO group (n=45, p
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- 2022
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13. Iron Porphyrin as a Cytochrome P450 Model for the Degradation of Dye
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Dan-Dan Ren, Xiaoyan Lu, Li-Ping Zhou, Huanghongjun Tian, Shuang Wang, Lu-Fang Ma, and Dong-Sheng Li
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iron porphyrins ,dye degradation ,gentian violet ,iodosylbenzene ,UV–vis ,Organic chemistry ,QD241-441 - Abstract
Organic dyes are widely used in the textile, biological, medical and other fields. However, a serious environmental problem has appeared because of the presence of organic dyes in industrial aqueous effluents. Thus, the efficient treatment of organic dyes in industrial wastewaters is currently in real demand. The current study investigated the oxidative degradation of the organic dye gentian violet by meso-tetra(carboxyphenyl) porphyriniron(III), [FeIII(TCPP)] as a cytochrome P450 model and iodosylbenzene (PhIO) as an oxidant at room temperature. The degradation reaction was monitored by UV–vis absorption spectroscopy via the observation of UV–vis spectral changes of the gentian violet. The results showed that the efficiency of catalyzed degradation reached more than 90% in 1 h, indicating the remarkable oxidative degradation capacity of the [FeIII(TCPP)]/PhIO system, which provided an efficient approach for the treatment of dyeing wastewater.
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- 2022
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14. The Lignan-Rich Fraction from Sambucus williamsii Hance Exerts Bone Protective Effects via Altering Circulating Serotonin and Gut Microbiota in Rats
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Hui-Hui Xiao, Yu-Xin Zhu, Lu Lu, Li-Ping Zhou, Christina Chui-Wa Poon, Chi-On Chan, Li-Jing Wang, Sisi Cao, Wen-Xuan Yu, Ka-Ying Wong, Daniel Kam-Wah Mok, and Man-Sau Wong
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osteoporosis ,lignans ,serotonin ,TPH-1 ,gut microbiota ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Our previous study revealed that the bone anabolic effects of the lignan-rich fraction (SWCA) from Sambucus williamsii Hance was involved in modulating the metabolism of tryptophan in vivo and inhibiting serotonin (5-HT) synthesis in vitro. This study aimed to determine how SWCA modulates bone metabolism via serotonin in vivo. The effects of SWCA were evaluated by using 4-month-old Sprague-Dawley (SD) ovariectomized rats. The serum levels of 5-HT and kynurenine, the protein expressions of tryptophan hydroxylase 1 (TPH-1) and TPH-2, the genes and proteins related to the 5-HT signaling pathway as well as gut microbiota composition were determined. SWCA treatment alleviated bone loss and decreased serum levels of serotonin, which was negatively related to bone mineral density (BMD) in rats. It suppressed the protein expression of TPH-1 in the colon, and reversed the gene and protein expressions of FOXO1 and ATF4 in the femur in OVX rats, while it did not affect the TPH-2 protein expression in the cortex. SWCA treatment escalated the relative abundance of Antinobacteria and modulated several genera relating to BMD. These findings verified that the bone protective effects of lignans were mediated by serotonin, and provided evidence that lignans might be a good source of TPH-1 inhibitors.
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- 2022
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15. The lignan-rich fraction from Sambucus Williamsii Hance ameliorates dyslipidemia and insulin resistance and modulates gut microbiota composition in ovariectomized rats
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Hui-Hui Xiao, Lu Lu, Christina Chui-Wa Poon, Chi-On Chan, Li-Jing Wang, Yu-Xin Zhu, Li-Ping Zhou, Sisi Cao, Wen-Xuan Yu, Ka Ying Wong, Daniel Kam-Wah Mok, and Man-Sau Wong
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Lignan-rich fraction ,Sambucus williamsii Hance ,Dyslipidemia ,Insulin resistance ,Ovariectomized rat ,Actinobacteria ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Menopausal women are susceptible to have high risk of cardiovascular diseases, type II diabetes and osteoporosis due to the metabolic disorder caused by estrogen deficiency. Accumulating evidence supports that gut microbiota is a key regulator of metabolic diseases. Our previous metabolomics study interestingly demonstrated that the anti-osteoporotic effects of lignan-rich fraction (SWCA) from Sambucus wialliamsii Hance were related to the restoration of a series of lipid and glucose metabolites. This study aims to investigate how SWCA modulates lipid and glucose metabolism and the underlying mechanism. Our results show that oral administration of SWCA (140 mg/kg and 280 mg/kg) for 10 weeks alleviated dyslipidemia, improved liver functions, prevented glucose tolerance and insulin actions, attenuated system inflammation and improved intestinal barrier in OVX rats. It also induced a high abundance of Actinobacteria, and restored microbial composition. We are the first to report the protective effects of the lignan-rich fraction from S. williamsii on dyslipidemia and insulin resistance. Our findings provide strong evidence for the application of this lignan-rich fraction to treat menopausal lipid disorder and insulin resistance-related diseases.
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- 2021
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16. Ginsenoside Rg1 activates ligand-independent estrogenic effects via rapid estrogen receptor signaling pathway
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Quan-Gui Gao, Li-Ping Zhou, Vien Hoi-Yi Lee, Hoi-Yi Chan, Cornelia Wing-Yin Man, and Man-Sau Wong
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Botany ,QK1-989 - Abstract
Background: Ginsenoside Rg1 was shown to exert ligand-independent activation of estrogen receptor (ER) via mitogen-activated protein kinase–mediated pathway. Our study aimed to delineate the mechanisms by which Rg1 activates the rapid ER signaling pathways. Methods: ER-positive human breast cancer MCF-7 cells and ER-negative human embryonic kidney HEK293 cells were treated with Rg1 (10−12M, 10−8M), 17ß-estradiol (10−8M), or vehicle. Immunoprecipitation was conducted to investigate the interactions between signaling protein and ER in MCF-7 cells. To determine the roles of these signaling proteins in the actions of Rg1, small interfering RNA or their inhibitors were applied. Results: Rg1 rapidly induced ERα translocation to plasma membrane via caveolin-1 and the formation of signaling complex involving linker protein (Shc), insulin-like growth factor-I receptor, modulator of nongenomic activity of ER (MNAR), ERα, and cellular nonreceptor tyrosine kinase (c-Src) in MCF-7 cells. The induction of extracellular signal-regulated protein kinase and mitogen-activated protein kinase kinase (MEK) phosphorylation in MCF-7 cells by Rg1 was suppressed by cotreatment with small interfering RNA against these signaling proteins. The stimulatory effects of Rg1 on MEK phosphorylation in these cells were suppressed by both PP2 (Src kinase inhibitor) and AG1478 [epidermal growth factor receptor (EGFR) inhibitor]. In addition, Rg1-induced estrogenic activities, EGFR and MEK phosphorylation in MCF-7 cells were abolished by cotreatment with G15 (G protein-coupled estrogen receptor-1 antagonist). The increase in intracellular cyclic AMP accumulation, but not Ca mobilization, in MCF-7 cells by Rg1 could be abolished by G15. Conclusion: Ginsenoside Rg1 exerted estrogenic actions by rapidly inducing the formation of ER containing signalosome in MCF-7 cells. Additionally, Rg1 could activate EGFR and c-Src ER-independently and exert estrogenic effects via rapid activation of membrane-associated ER and G protein-coupled estrogen receptor. Keywords: Estrogen receptor, Estrogen receptor signaling proteins, Ginsenoside Rg1, G protein-coupled estrogen receptor-1, Mitogen-activated protein kinase
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- 2019
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17. Electronic Properties of Armchair Black Phosphorene Nanoribbons Edge-Modified by Transition Elements V, Cr, and Mn
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Jiong-Hua Huang, Xue-Feng Wang, Yu-Shen Liu, and Li-Ping Zhou
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Black phosphorene nanoribbons ,Half semiconductor ,Spin diode ,Semiconductor-metal transition ,Edge functionalization ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Abstract The structural, electrical, and magnetic properties of armchair black phosphorene nanoribbons (APNRs) edge-functionalized by transitional metal (TM) elements V, Cr, and Mn were studied by the density functional theory combined with the non-equilibrium Green’s function. Spin-polarized edge states introduce great varieties to the electronic structures of TM-APNRs. For APNRs with Mn-stitched edge, their band structures exhibit half-semiconductor electrical properties in the ferromagnetic state. A transverse electric field can then make the Mn-APNRs metallic by shifting the conduction bands of edge states via the Stark effect. The Mn/Cr-APNR heterojunction may be used to fabricate spin p-n diode where strong rectification acts only on one spin.
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- 2019
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18. Two New Approaches for Multi-Attribute Group Decision-Making With Interval-Valued Neutrosophic Frank Aggregation Operators and Incomplete Weights
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Li-Ping Zhou, Jiu-Ying Dong, and Shu-Ping Wan
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Multi-attribute group decision-making ,interval-valued neutrosophic set ,interval-valued neutrosophic frank weighted averaging operator ,interval-valued neutrosophic frank weighted geometric operator ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
This paper investigates some Frank aggregation operators of interval-valued neutrosophic numbers (IVNNs) and applies to multi-attribute group decision-making (MAGDM) problems. First, the Frank t-conorm and t-norm are extended to interval-valued neutrosophic environment. Some new operational laws for IVNNs are defined and their related properties are investigated. Based on these new operational laws, some new aggregation operators for IVNNs are developed including the interval-valued neutrosophic Frank weighted averaging (IVNFWA) operator and the interval-valued neutrosophic Frank weighted geometric (IVNFWG) operator. Then some desirable properties and special cases of these new operators are further discussed. To solve the MAGDM with IVNNs, the weights of decision makers (DMs) are determined by using extended technique for order preference by similarity to ideal solution (TOPSIS) method based on cross-entropy. Additionally, attribute weights are determined based on the similarity degrees between alternatives and the absolute ideal solutions. Further, two new decision-making approaches for MAGDM with IVNNs are put forward by means of the IVNFWA and IVNFWG operators, respectively. Finally, a case study of selecting an agricultural socialization service provider is analyzed to illustrate the practicality and effectiveness of the developed two approaches.
- Published
- 2019
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19. Polymer Scaffolds for Biomedical Applications in Peripheral Nerve Reconstruction
- Author
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Meng Zhang, Ci Li, Li-Ping Zhou, Wei Pi, and Pei-Xun Zhang
- Subjects
peripheral nerve injury ,nerve conduits ,nerve reconstruction ,polymeric scaffold ,natural and synthetic polymers ,Organic chemistry ,QD241-441 - Abstract
The nervous system is a significant part of the human body, and peripheral nerve injury caused by trauma can cause various functional disorders. When the broken end defect is large and cannot be repaired by direct suture, small gap sutures of nerve conduits can effectively replace nerve transplantation and avoid the side effect of donor area disorders. There are many choices for nerve conduits, and natural materials and synthetic polymers have their advantages. Among them, the nerve scaffold should meet the requirements of good degradability, biocompatibility, promoting axon growth, supporting axon expansion and regeneration, and higher cell adhesion. Polymer biological scaffolds can change some shortcomings of raw materials by using electrospinning filling technology and surface modification technology to make them more suitable for nerve regeneration. Therefore, polymer scaffolds have a substantial prospect in the field of biomedicine in future. This paper reviews the application of nerve conduits in the field of repairing peripheral nerve injury, and we discuss the latest progress of materials and fabrication techniques of these polymer scaffolds.
- Published
- 2021
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20. A new method to solve anophthalmic contracted socket in embedding orbital implant in 114 cases
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Ming Cheng, Li-Ping Zhou, Jia Li, and Hong-Feng Yuan
- Subjects
anophthalmia ,contracted socket ,orbital implant ,Ophthalmology ,RE1-994 - Abstract
AIM: To perform a new method for orbital implant and contracted socket through one time and its results.METHODS: Totally 114 patients 114 eyes, from January 2008 to June 2014, with contracted socket participated in this study. We incised the bulbar conjunctiva horizontally and excised scar tissue, then implanted the hydroxyapatite in the four extraocular muscles and tightly sutured the Tenon' capsule. After that, we put the superior and inferior conjunctival petals backwards and sutured them to the Tenon's capsule. All the patients were divided into four groups according to the vertical diameter length of the conjunctival defect area: Group Ⅰ: ≤5mm; Group Ⅱ: 6-10mm; Group Ⅲ: 11-15mm; and Group Ⅳ: ≥16mm. These patients were followed up for 6mo to 3y to observe the conjunctival sac shaping and growth of conjunctiva.RESULTS: There were 64 cases in Group Ⅰ, 31 cases in Group Ⅱ, 16 cases in Group Ⅲ and 3 cases in Group Ⅳ. All patients' conjunctival defect was covered by new conjunctiva and scar tissue 4 to 6wk after surgeries. Ten cases had contracted socket; 2 cases had orbital implant exposure, requiring reoperation. Of the 114 cases, 8 had contracted socket and could use a smaller conformer, 106 could use a normal size conformer.CONCLUSION: When the conjunctival defect was ≤15mm, this new method can address the orbital implant and contracted socket at the same time. While it was ≥16mm, flap transplantation is necessary.
- Published
- 2017
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21. Clinical diagnosis and treatment of intraorbital wooden foreign bodies
- Author
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Jia Li, Li-Ping Zhou, Jing Jin, and Hong-Feng Yuan
- Subjects
Eye injuries ,Wooden foreign bodies ,Computed tomography ,Magnetic resonance imaging ,Medicine (General) ,R5-920 - Abstract
Purpose: The intraorbital wooden foreign body is often misdiagnosed or missed on computed tomography (CT) scan, due to the invisible or unclear images. The residual foreign bodies often occur during surgical removal. The clinical manifestations, imaging features and treatment of intraorbital wooden foreign bodies were discussed in this study. Method: We retrospectively analyzed 14 cases of intraorbital wooden foreign bodies managed at our hospital between January 2007 and May 2015. All patients underwent orbital CT examination before surgery, and surgery was performed under general anesthesia with orbital wound debridement and suture, as well as exploration and removal of wooden foreign bodies. Results: At first, 11 cases underwent removal of foreign bodies, including 1 case with incomplete removal and then receiving a secondary surgery. Foreign bodies were not found in three cases with preoperative misdiagnosis and orbital MRI found residual foreign bodies in the orbit. Operations were performed via primary wound approach in eight cases, conjunctival approach in two cases, and anterior orbitotomy in four cases. Postoperatively, one case was complicated with eye injuries, three cases with ocular muscle injuries, eight cases with visual loss, and eight cases with orbital abscess. The length of foreign bodies ranged from 1.8 cm to 11.0 cm. The maximum of four foreign bodies were removed at the same time. Conclusion: Because the imaging of orbital wooden foreign bodies is complex and varied, MRI should be combined when they are invisible on CT scan. At the same time injuries trajectory and clinical manifestations of patients should be taken into account. Surgical exploration should be extensive and thorough, and foreign bodies and orbital abscess must be cleared.
- Published
- 2016
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22. Oleanolic Acid and Ursolic Acid Improve Bone Properties and Calcium Balance and Modulate Vitamin D Metabolism in Aged Female Rats
- Author
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Sisi Cao, Xue-Lian Tian, Wen-Xuan Yu, Li-Ping Zhou, Xiao-Li Dong, Murray J. Favus, and Man-Sau Wong
- Subjects
oleanolic acid ,ursolic acid ,bone protection ,calcium balance ,vitamin D metabolism ,aged rats ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Oleanolic acid (OA) and ursolic acid (UA) are the major chemical constituents in Fructus Ligustri Lucidi (FLL), a kidney-tonifying Chinese herb that is previously shown to improve bone properties and enhance calcium balance in aged female rats. The present study was designed to study if OA and UA act as the active ingredients in FLL to exert the positive effects on bone and mineral metabolism in aged rats. Aged (13-month-old) Sprague-Dawley female rats were randomly assigned to four groups with oral administration of drug or vehicle treatment for 12 weeks: medium calcium diet (MCD, 0.6% calcium), high calcium diet (HCD, 1.2% calcium), MCD + FLL (700 mg/kg/day), MCD + OA (23.6 mg/kg/day) + UA (8.6 mg/kg/day). A group of mature (3-month-old) female rats fed with MCD was included as positive control. The results demonstrated that FLL and OA+UA increased bone mineral density and improved microarchitectural properties of aged female rats. The osteoprotective effects of FLL and OA+UA might be, at least in part, associated with their actions on enhancing calcium balance and suppressing age-induced secondary hyperparathyroidism in aged female rats. FLL and OA+UA also significantly induced renal CYP27B1 protein expression and OA+UA treatment decreased CYP24A1 mRNA and protein expressions in aged female rats. In addition, FLL and OA+UA significantly increased the promoter activity, mRNA and protein expressions of renal CYP27B1 in vitro in human proximal tubule HKC-8 cells. The present findings suggest that OA+UA can be regarded as the active ingredients of FLL and might be a potential drug candidate for prevention and treatment of osteoporosis.
- Published
- 2018
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23. Effects of long-term full straw return on yield and potassium response in wheat-maize rotation
- Author
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You-lu BAI, Lei WANG, Yan-li LU, Li-ping YANG, Li-ping ZHOU, Lu NI, and Ming-fang CHENG
- Subjects
straw return ,potassium in straw ,wheat ,maize ,potassium response ,Agriculture (General) ,S1-972 - Abstract
The effect of long-term straw return on crop yield, soil potassium (K) content, soil organic matter, and crop response to K from both straw and chemical K fertilizer (K2SO4) were investigated in a fixed site field experiment for winter wheat-summer maize rotation in 6 years for 12 seasons. The field experiment was located in northern part of North China Plain with a sandy soil in relatively low yield potential. Two factors, straw return and chemical K fertilizer, were studied with two levels in each factor. Field split design was employed, with two straw treatments, full straw return of previous crop (St) and no straw return, in main plots, and two chemical K fertilizer treatments, 0 and 60 kg K2O ha−1, as sub-plots. The results showed that straw return significantly increased yields of winter wheat and summer maize by 16.5 and 13.2% in average, respectively, and the positive effect of straw return to crop yield showed more effective in lower yield season. Straw return significantly increased K absorption by the crops, with significant increase in straw part. In treatment with straw return, the K content in crop straw increased by 15.9 and 21.8% in wheat and maize, respectively, compared with no straw return treatment. But, straw return had little effect on K content in grain of the crops. Straw return had significant influences on total K uptake by wheat and maize plants, with an increase of 32.7 and 30.9%, respectively. There was a significant correlation between crop yield and K uptake by the plant. To produce 100 kg grain, the wheat and maize plants absorbed 3.26 and 2.24 kg K2O, respectively. The contents of soil available K and soil organic matter were significantly affected by the straw return with an increase of 6.07 and 23.0%, respectively, compared to no straw return treatment. K2SO4 application in rate of 60 kg K2O ha−1 showed no significant effect on wheat and maize yield, K content in crop straw, total K uptake by the crops, soil available K content, and soil organic matter. The apparent K utilization rate (percentage of applied K absorbed by the crop in the season) showed difference for wheat and maize with different K sources. In wheat season, the K utilization rate from K2SO4 was higher than that from straw, while in maize season, the K utilization rate from straw was higher than that from chemical fertilizer. In the whole wheat-maize rotation system, the K absorption efficiency by the two crops from straw was higher than that from K2SO4.
- Published
- 2015
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24. Hyper-spectral characteristics and classification of farmland soil in northeast of China
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Yan-li LU, You-lu BAI, Li-ping YANG, Lei WANG, Yi-lun WANG, Lu NI, and Li-ping ZHOU
- Subjects
soil type ,spectral characteristics ,principle component ,classification ,Agriculture (General) ,S1-972 - Abstract
The physical and chemical heterogeneities of soils make the soil spectral different and complicated, and it is valuable to increase the accuracy of prediction models for soil organic matter (SOM) based on pre-classification. This experiment was conducted under a controllable environment, and different soil samples from northeast of China were measured using ASD2500 hyperspectral instrument. The results showed that there are different reflectances in different soil types. There are statistically significant correlation between SOM and reflectence at 0.05 and 0.01 levels in 550–850 nm, and all soil types get significant at 0.01 level in 650–750 nm. The results indicated that soil types of the northeast can be divided into three categories: The first category shows relatively flat and low reflectance in the entire band; the second shows that the spectral reflectance curve raises fastest in 460–610 nm band, the sharp increase in the slope, but uneven slope changes; the third category slowly uplifts in the visible band, and its slope in the visible band is obviously higher than the first category. Except for the classification by curve shapes of reflectance, principal component analysis is one more effective method to classify soil types. The first principal component includes 62.13–97.19% of spectral information and it mainly relates to the information in 560–600, 630–690 and 690–760 nm. The second mainly represents spectral information in 1 640–1 740, 2 050–2 120 and 2 200–2 300 nm. The samples with high OM are often in the left, and the others with low OM are in the right of the scatter plot (the first principal component is the horizontal axis and the second is the longitudinal axis). Soil types in northeast of China can be classified effectively by those two principles; it is also a valuable reference to other soil in other areas.
- Published
- 2015
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25. Determining the Age of Terrace Formation Using Luminescence Dating—A Case of the Yellow River Terraces in the Baode Area, China
- Author
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Jia-Fu Zhang, Wei-Li Qiu, Gang Hu, and Li-Ping Zhou
- Subjects
terrace strath and tread ages ,luminescence dating ,deposition rate ,age-depth model ,yellow river terrace ,Biology (General) ,QH301-705.5 - Abstract
Dating fluvial terraces has long been a challenge for geologists and geomorphologists, because terrace straths and treads are not usually directly dated. In this study, the formation ages of the Yellow River terraces in the Baode area in China were determined by dating fluvial deposits overlying bedrock straths using optically stimulated luminescence (OSL) dating techniques. Seven terraces (from the lowest terrace T1 to the highest terrace T7) in the study area were recognized, and they are characterized by thick fluvial terrace deposits overlaid by loess sediments. Twenty-five samples from nine terrace sections were dated to about 2−200 ka. The OSL ages (120−190 ka) of the fluvial samples from higher terraces (T3−T6) seem to be reliable based on their luminescence properties and stratigraphic consistency, but the geomorphologic and stratigraphic evidence show that these ages should be underestimated, because they are generally similar to those of the samples from the lower terrace (T2). The formation ages of the terrace straths and treads for the T1 terrace were deduced to be about 44 ka and 36 ka, respectively, based on the deposition rates of the fluvial terrace deposits, and the T2 terrace has the same strath and tread formation age of about 135 ka. The incision rate was calculated to be about 0.35 mm/ka for the past 135 ka, and the uplift rate pattern suggests that the Ordos Plateau behaves as a rigid block. Based on our previous investigations on the Yellow River terraces and the results in this study, we consider that the formation ages of terrace straths and treads calculated using deposition rates of terrace fluvial sediments can overcome problems associated with age underestimation or overestimation of strath or fill terraces based on the single age of one fluvial terrace sample. The implication is that, for accurate dating of terrace formation, terrace sections should be systematically sampled and dated.
- Published
- 2020
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26. Bone Protective Effects of Danggui Buxue Tang Alone and in Combination With Tamoxifen or Raloxifene in vivo and in vitro
- Author
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Li-Ping Zhou, Ka-Ying Wong, Hoi-Ting Yeung, Xiao-Li Dong, Hui-Hui Xiao, Amy G.-W. Gong, Karl W.-K. Tsim, and Man-Sau Wong
- Subjects
Danggui Buxue Tang ,SERMs ,postmenopausal osteoporosis ,interactions ,estrogen receptor ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Danggui Buxue Tang (DBT), a traditional Chinese Medicine decoction containing Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), is commonly prescribed for women in China as a remedy for menopausal symptoms. Previous study indicated that DBT stimulated cell growth and differentiation of human osteosarcoma MG-63 cells and exhibited estrogenic properties via estrogen receptors (ERs). The present study aimed to study the bone protective effects of DBT and its potential interactions with selective estrogen receptor modulators (SERMs, tamoxifen and raloxifene) in both in vivo and in vitro models as they act via similar ERs. Six-month-old Sprague-Dawley rats were randomly assigned to the following treatments for 12 weeks: (1) sham-operated control group with vehicle (sham), (2) ovariectomized group with vehicle (OVX), (3) OVX with 17β-estradiol (E2, 2.0 mg/kg day), (4) OVX with tamoxifen (Tamo, 1.0 mg/kg day), (5) OVX with raloxifene (Ralo, 3.0 mg/kg day), (6) OVX with DBT (DBT, 3.0 g/kg day), (7) OVX with DBT+Tamoxifen (DBT+Tamo), and (8) OVX with DBT+Raloxifene (DBT+Ralo). Effects of DBT and potential interactions between DBT and SERMs were also evaluated in MG-63 cells. DBT, tamoxifen, raloxifene, and their combinations significantly increased bone mineral density (BMD) and improved trabecular bone properties, including bone surface (BS), trabecular bone number (Tb.N), and trabecular bone separation (Tb.Sp), as well as restored changes in bone turnover biomarkers and mRNA expression of genes involved in bone metabolism in OVX rats. Furthermore, DBT, SERMs, and their combinations significantly increased serum estradiol and suppressed follicle stimulating hormone and luteinizing hormone in OVX rats, suggesting the possible involvement of the hypothalamus–pituitary–gonadal axis in mediating their bone protective effects. However, SERMs, but not DBT, significantly increased uterus index in OVX rats. DBT significantly induced ALP activity and estrogen response element-dependent transcription in MG-63 cells. Our study demonstrated that DBT alone and in combinations with SERMs could exert bone protective effects in vitro and in vivo.
- Published
- 2018
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27. miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2
- Author
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Ya-Han Wu, Hong Zhao, Li-Ping Zhou, Chun-Xia Zhao, Yu-Fei Wu, Li-Xiao Zhen, Jun Li, Dong-Xia Ge, Liang Xu, Li Lin, Yi Liu, Dan-Dan Liang, and Yi-Han Chen
- Subjects
hCMPCs ,miR-134 ,proliferation ,Meis2 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Cardiomyocyte progenitor cells play essential roles in early heart development, which requires highly controlled cellular organization. microRNAs (miRs) are involved in various cell behaviors by post-transcriptional regulation of target genes. However, the roles of miRNAs in human cardiomyocyte progenitor cells (hCMPCs) remain to be elucidated. Our previous study showed that miR-134 was significantly downregulated in heart tissue suffering from congenital heart disease, underlying the potential role of miR-134 in cardiogenesis. In the present work, we showed that the upregulation of miR-134 reduced the proliferation of hCMPCs, as determined by EdU assay and Ki-67 immunostaining, while the inhibition of miR-134 exhibited an opposite effect. Both up- and downregulation of miR-134 expression altered the transcriptional level of cell-cycle genes. We identified Meis2 as the target of miR-134 in the regulation of hCMPC proliferation through bioinformatic prediction, luciferase reporter assay and western blot. The over-expression of Meis2 mitigated the effect of miR-134 on hCMPC proliferation. Moreover, miR-134 did not change the degree of hCMPC differentiation into cardiomyocytes in our model, suggesting that miR-134 is not required in this process. These findings reveal an essential role for miR-134 in cardiomyocyte progenitor cell biology and provide new insights into the physiology and pathology of cardiogenesis.
- Published
- 2015
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28. 7-Diethylamino-3-{(E)-4-[(E)-2-(pyridin-4-yl)ethenyl]styryl}-2H-chromen-2-one
- Author
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Li-Ping Zhou and Ling-Liang Long
- Subjects
Crystallography ,QD901-999 - Abstract
In the title coumarin derivative, C28H26N2O2, the coumarin unit is approximately planar, with a maximum deviation of 0.048 (3) Å. The central benzene ring is oriented at dihedral angles of 30.15 (14) and 10.51 (11)°, respectively, to the pyridine ring and coumarin ring system. In the crystal, weak C—H...O and C—H...N hydrogen bonds and weak C—H...π interactions link the molecules into a three-dimensional supramolecular architecture.
- Published
- 2014
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29. Section curve reconstruction and mean-camber curve extraction of a point-sampled blade surface.
- Author
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Wen-long Li, He Xie, Qi-dong Li, Li-ping Zhou, and Zhou-ping Yin
- Subjects
Medicine ,Science - Abstract
The blade is one of the most critical parts of an aviation engine, and a small change in the blade geometry may significantly affect the dynamics performance of the aviation engine. Rapid advancements in 3D scanning techniques have enabled the inspection of the blade shape using a dense and accurate point cloud. This paper proposes a new method to achieving two common tasks in blade inspection: section curve reconstruction and mean-camber curve extraction with the representation of a point cloud. The mathematical morphology is expanded and applied to restrain the effect of the measuring defects and generate an ordered sequence of 2D measured points in the section plane. Then, the energy and distance are minimized to iteratively smoothen the measured points, approximate the section curve and extract the mean-camber curve. In addition, a turbine blade is machined and scanned to observe the curvature variation, energy variation and approximation error, which demonstrates the availability of the proposed method. The proposed method is simple to implement and can be applied in aviation casting-blade finish inspection, large forging-blade allowance inspection and visual-guided robot grinding localization.
- Published
- 2014
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30. A Fermatean Fuzzy ELECTRE Method for Multi-Criteria Group Decision-Making.
- Author
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Li-Ping Zhou, Shu-Ping Wan, and Jiu-Ying Dong
- Published
- 2022
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31. A control strategy for extractive and reactive dividing wall columns
- Author
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Rodríguez, Manuel, Li, Ping Zhou, and Díaz, Ismael
- Published
- 2017
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32. Anti-Colon Cancer Activity of Patrinia villosa Extract and Analysis of its Components based on UPLC-QTOF-MS/MS
- Author
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Xin Xin Yang, Shuai Wang, Li Ping Zhou, Tian Jiao Li, Lin Zhao, Xian Sheng Meng, Feng Gao, and Yong Rui Bao
- Subjects
Pharmacology - Published
- 2022
33. Hazard rate for postoperative recurrence in patients with hepatocellular carcinoma at Barcelona Clinic Liver Cancer stage 0 or A1: A multicenter observational study
- Author
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Yan‐Jun Xiang, Kang Wang, Hong‐Ming Yu, Miao‐Miao Wang, Le‐Qun Li, Hui‐Chuan Sun, Tian‐Fu Wen, Yu‐Qing Zhang, Yun‐Feng Shan, Li‐Ping Zhou, and Shu‐Qun Cheng
- Subjects
Infectious Diseases ,Hepatology - Abstract
Surgical treatment is the first-line treatment for patients with Barcelona Clinic Liver Cancer (BCLC) stage 0 or A1 hepatocellular carcinoma (HCC), and postoperative monitoring improves long-term survival. We aimed to establish a reasonable short-interval follow-up duration for patients with HCC.The cohort for this retrospective study included 1396 HCC patients with BCLC stage 0 or A1 disease who underwent curative resection from 2013 to 2016 at five centers in China. Hazard rates for recurrence were calculated using the hazard function.The recurrence rates in patients with BCLC stage 0 and A1 HCC were 46.4% and 58.0%, respectively. The hazard curve for stage 0 patients was relatively flat, and the hazard rate was consistently low (peak hazard rate 0.0163). The hazard rate curve for recurrence was initially high (peak hazard rate 0.0441) in patients with BCLC stage A1 disease and showed a rapid decreasing trend within 1 year, followed by a slow decreasing trend, reaching a low level (0.0163) at approximately 36 months. The time to low risk was 47, 41, and 51 months in patients with cirrhosis, hepatitis B virus (HBV) infection, and satellite lesions, respectively.A short-interval follow-up of 1 year is sufficient for HCC patients with BCLC stage 0 disease, whereas a short-interval follow-up time of 3 years should be considered for patients with stage A1 disease. The follow-up period should be appropriately prolonged for patients with cirrhosis, HBV infection, and satellite lesions.
- Published
- 2022
34. Transarterial chemoembolization plus a PD‐1 inhibitor with or without lenvatinib for intermediate‐stage hepatocellular carcinoma
- Author
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Yan‐Jun Xiang, Kang Wang, Hong‐Ming Yu, Xiao‐Wei Li, Yu‐Qiang Cheng, Wei‐Jun Wang, Jin‐Kai Feng, Meng‐Han Bo, Ying‐Yi Qin, Yi‐Tao Zheng, Yun‐Feng Shan, Li‐Ping Zhou, Jian Zhai, and Shu‐Qun Cheng
- Subjects
Infectious Diseases ,Hepatology - Abstract
Transarterial chemoembolization (TACE) combined with a PD-1 inhibitor and TACE combined with a PD-1 inhibitor and lenvatinib have recently been reported as promising treatments to improve the prognosis of hepatocellular carcinoma (HCC) patients. This study aims to compare the efficacy of these two treatments.A retrospective study was conducted, and patients were recruited from two centers in China. Progression-free survival (PFS) and overall survival (OS) were compared, and the objective response rate (ORR) and disease control rate (DCR) were evaluated according to the modified Response Evaluation Criteria in Solid Tumors (mRECIST). Treatment-related adverse events (AEs) were analyzed to assess safety.The median follow-up for the entire cohort was 11.4 months. Of the 103 patients included in this study, 56 received triple therapy, and 47 received doublet therapy. PFS was significantly higher in the triple therapy group than in the doublet therapy group (mPFS 22.5 vs. 14.0 months, P 0.001). Similar results were obtained in terms of OS (P = 0.001). The ORR and DCR were also better in the triple therapy group (64.3% vs. 38.3%, P = 0.010; 85.7% vs. 57.4%, P = 0.002). The most common AEs in the triple therapy group were decreased albumin (55.3%), decreased platelet count (51.8%) and hypertension (44.6%).The combination of TACE with a PD-1 inhibitor and lenvatinib in patients with BCLC stage B HCC might result in significantly improved clinical outcomes with a manageable safety profile compared with TACE with a PD-1 inhibitor.
- Published
- 2022
35. Efficacy and safety of <scp>TACE</scp> combined with lenvatinib and <scp>PD</scp> ‐1 inhibitors for unresectable recurrent <scp>HCC</scp> : A multicenter, retrospective study
- Author
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Wei‐Jun Wang, Zong‐Han Liu, Kang Wang, Hong‐Ming Yu, Yu‐Qiang Cheng, Yan‐Jun Xiang, Jin‐Kai Feng, Li‐Ping Zhou, Hong‐Kun Zhou, Wei‐Wei Pan, Wei‐Xing Guo, Jie Shi, and Shu‐Qun Cheng
- Subjects
Cancer Research ,Oncology ,Radiology, Nuclear Medicine and imaging - Published
- 2023
36. A Fermatean Fuzzy ELECTRE Method for Multi-Criteria Group Decision-Making
- Author
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Jiu-Ying Dong, Li-Ping Zhou, and Shu-Ping Wan
- Subjects
Operations research ,Computer science ,Multi criteria ,Applied Mathematics ,ELECTRE ,Fuzzy logic ,Information Systems ,Group decision-making - Abstract
This paper aims to develop a Fermatean fuzzy ELECTRE method for solving multi-criteria group decision-making problems with unknown weights of decision makers and incomplete weights of criteria. First, a new distance measure between Fermatean fuzzy sets is proposed based on the Jensen–Shannon divergence. The cross entropy for Fermatean fuzzy sets is defined. Three kinds of dominance relationships for Fermatean fuzzy sets are proposed. Then, two optimization models are constructed to obtain positive ideal decision-making information and negative ideal decision-making information, respectively. Accordingly, the credibility degree of each decision maker is calculated. Decision makers’ dynamic weights are determined by their credibility degrees. Besides, to obtain the weights of criteria, an optimization model is constructed based on grey relational analysis for Fermatean fuzzy numbers. Finally, the strong, medium and weak Fermatean fuzzy concordance and discordance sets are identified to construct the Fermatean fuzzy concordance and discordance matrices, respectively. A practical case study is carried out to illustrate the feasibility and applicability of the proposed ELECTRE method. Comparative analyses are performed to demonstrate the superiority and effectiveness of the proposed ELECTRE method.
- Published
- 2021
37. [Effect of Maxing Shigan Decoction and dissembled prescriptions against airway inflammation in RSV-aggravated asthma and mechanism of regulating TRPV1]
- Author
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Meng-Wen, Li, Xin-Sheng, Fan, Li-Ping, Zhou, Mo, Liu, and Er-Xin, Shang
- Subjects
Inflammation ,Mice, Inbred BALB C ,Interleukin-13 ,Ovalbumin ,TRPV Cation Channels ,Dinoprostone ,Asthma ,Mice, Inbred C57BL ,Mice ,Disease Models, Animal ,Prescriptions ,Animals ,Female ,Interleukin-4 ,RNA, Messenger ,Lung ,Bronchoalveolar Lavage Fluid - Abstract
This study investigated the effect of Maxing Shigan Decoction(MXSGD) and its disassembled prescriptions against the airway inflammation in respiratory syncytial virus(RSV)-aggravated asthma and the regulation of transient receptor potential vanilloid-1(TRPV1). To be specific, ovalbumin(OVA) and RSV were used to induce aggravated asthma in mice(female, C57BL/6). Then the model mice were intervened by MXSGD and the disassembled prescriptions. The eosinophil(EOS) in peripheral blood, inflammatory cells in bronchoalveolar lavage fluid(BALF), enhanced pause(Penh) variation, and lung pathological damage in each group were observed, and the changes of interleukin(IL)-4, IL-13, substance P(SP), and prostaglandin E2(PGE2) in BALF were mea-sured by enzyme-linked immunosorbent assay(ELISA). Quantitative real time polymerase chain reaction(qPCR) and Western blot were used to detect mRNA and protein of TRPV1 in mouse lung tissue. In the in vitro experiment, 16 HBE cells were stimulated with IL-4 and RSV. Then the changes of TRPV1 expression after the intervention with the serum containing MXSGD and its disassembled prescriptions were observed. Besides, the intracellular Ca~(2+) level after the stimulation with TRPV1 agonist was evaluated. The results showed that the mice in the model group had obvious asthma phenotype, the levels of various inflammatory cells in the peripheral blood and BALF and Penh were significantly increased(Plt;0.05, Plt;0.01), and the lung tissue was severely damaged compared with the control group. Compared with the model group, the levels of EOS in the peripheral blood and BALF were significantly decreased in the MXSGD group, the SG group and the MXC group(Plt;0.05, Plt;0.01). The levels of WBC and neutrophils in BALF were significantly decreased in the MXSGD group and SG group(Plt;0.01), the levels of neutrophils in BALF were decreased in the MXC group(Plt;0.05). The improvement effect of the MXGSD on the level of inflammatory cells in peripheral blood and BALF was better than that of two disassembled groups(Plt;0.05, Plt;0.01). After 50 mg·mL~(-1) acetylcholine chloride stimulation, the Penh values of the MXSGD group and the MXC group significantly decreased(Plt;0.01), and the Penh value of the SG group decreased(Plt;0.05). The levels of IL-4, IL-13, PGE2 and SP in BALF could be significantly decreased in the MXSGD group(Plt;0.05, Plt;0.01), the levels of IL-13 and PGE2 in BALF could be decreased in the MXC group(Plt;0.05, Plt;0.01), and the levels of IL-13, PGE2 and SP in BALF could be decreased in the SG group(Plt;0.05, Plt;0.01). MXSGD could down-regulate the protein and mRNA expression of TRPV1 in lung tissue(Plt;0.05, Plt;0.01). The serum containing MXSGD and its disassembled prescriptions could down-regulate TRPV1 expression in 16 HBE cells stimulated by IL-4 combined with RSV and inhibit the inward flow of Ca~(2+) induced by TRPV1 agonist, especially the serum containing MXSGD which showed better effect than the serum containing disassembled ones(Plt;0.05). In vivo and in vitro experiments verified the protective effect of MXSGD and its disassembled prescriptions against airway inflammation in RSV-exacerbated asthma, the whole decoction thus possessed synergy in treating asthma, with better performance than the dissembled prescriptions. Different groups of prescription had made contributions in improving airway hyperresponsiveness, anti-allergy and anti-inflammation. The mechanism is the likelihood that it regulates TRPV1 channel and levels of related inflammatory mediators.
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- 2022
38. Aviation Blade Inspection Based on Optical Measurement.
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Wen-Long Li, Li-Ping Zhou, and Youlun Xiong
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- 2013
- Full Text
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39. Sarcopenia is a predictive factor of poor quality of life and prognosis in patients after radical gastrectomy
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Feng-Min Zhang, Zi-Le Shen, Xia-Lin Yan, Hong-Bo Zou, Li-Ping Zhou, Zhen Yu, Cheng-Le Zhuang, Wen-Xi Dong, Ding-Ye Yu, and Guo-Jun Cai
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Male ,Sarcopenia ,medicine.medical_specialty ,Adenocarcinoma ,Anxiety ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Quality of life ,Gastrectomy ,Stomach Neoplasms ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Risk factor ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Radical gastrectomy ,business.industry ,Incidence (epidemiology) ,Cancer ,Health Care Costs ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,humanities ,Predictive factor ,Survival Rate ,Oncology ,030220 oncology & carcinogenesis ,Quality of Life ,Female ,Surgery ,medicine.symptom ,business ,human activities - Abstract
Background Patients with gastric cancer often suffer from generalized and progressive reduction of skeletal muscle mass and strength, which negatively affects the quality of life (QOL). In this study, we explored the impact of sarcopenia on QOL and overall survival (OS). Methods From December 2015 to June 2017, 135 patients underwent radical gastrectomy at the First Affiliated Hospital of Wenzhou Medical University. Based on the diagnostic criteria of the Asian Working Group for Sarcopenia (AWGS), data including handgrip strength, 6-m gait speed and muscle mass were collected and analyzed. EORTC QLQ-C30 and EORTC QLQ-STO22 were used to evaluate the QOL before surgery, 1, 3 and 6 months after surgery. Results A total of 27 out of the 135 patients (20.00%) were diagnosed with sarcopenia. Compared with non-sarcopenia group, patients in sarcopenia group had a higher incidence of postoperative complications (14.80% vs. 40.70%, p = 0.003), and more hospitalization costs (p = 0.029). The scores of eating restriction (p = 0.026), anxiety (p = 0.045) and body image (p = 0.046) were significantly higher in sarcopenia group at postoperative 6 months. Besides, sarcopenia was an independent risk factor for global health status at 6 months after operation (OR: 2.881, 95% CI: 1.110–7.475, p = 0.030) and OS (HR: 3.140, 95% CI: 1.255–7.855, p = 0.014). Other factors, including tumor stage III and the postoperative complications, had negative influences on OS. Conclusion Sarcopenia is a predictive factor of poor QOL and prognosis in patients with gastric cancer.
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- 2021
40. Gemcitabine plus Oxaliplatin (GEMOX) combined with Atezolizumab and Bevacizumab for Advanced Biliary Tract Cancer: A Preliminary Observational Study
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Kang Wang, Zong-Han Liu, Hong-Ming Yu, Yu-Qiang Cheng, Yan-Jun Xiang, Jing-Ya Zhong, Qian-Zhi Ni, Li-Ping Zhou, Chao Liang, Hong-Kun Zhou, Wei-Wei Pan, Wei-Xing Guo, Jie Shi, Wan Yee Lau, and Shu-Qun Cheng
- Abstract
Background: Anti-PD-L1/VEGF inhibition, coupled with chemotherapy, may potentiate antitumor immunity leading to enhanced clinical benefit, but it has not been investigated in advanced biliary tract cancer (BTC). We investigated the efficacy and safety of gemcitabine plus oxaliplatin (GEMOX) combined with atezolizumab and bevacizumab for advanced BTC and explore the potential biomarkers related with response. Patients and Methods: Advanced BTC patients, who received a triple combination therapy at three medical centers between March 18th, 2020 and Sep 1st, 2021, were included. Treatment response was evaluated via mRECIST and RECIST v1.1. Endpoints included the overall response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS) and safety. The whole exome sequencing of pathological tissues was conducted for bioinformatic analysis. Results: Thirty patients were enrolled ultimately in this study. The best ORR was 76.7% and the disease control rate was 90.0%. The median PFS was 12.0 months, and the median OS was not reached. During the treatment, 10.0% (3/30) patients suffered from ≥ grade 3 treatment-related adverse events (TRAEs). Furthermore, fever (73.3%), neutropenia (63.3%), AST and ALT level increased (50.0% and 43.3% respectively) are the most common TRAEs. Bioinformatics analysis revealed patients with altered ALS2CL had a higher ORR. Conclusion: The triple combination of atezolizumab, bevacizumab and GEMOX is potentially efficacious and safe for patients with advanced BTC. The mutation of ALS2CL is a potential predictive biomarker for the efficacy of triple combination therapy.
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- 2022
41. Effects of cigarette smoke on the aggravation of ovalbumin-induced asthma and the expressions of TRPA1 and tight junctions in mice
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Mo Liu, Qin-fei Yue, Yi-ming Zhang, Fang-yuan Hu, Yu-bo Sun, Li-ping Zhou, Xin-Sheng Fan, and Mengwen Li
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0301 basic medicine ,medicine.medical_specialty ,Ovalbumin ,Immunology ,Occludin ,Tight Junctions ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Smoke ,Internal medicine ,Tobacco ,medicine ,Animals ,TRPA1 Cation Channel ,Molecular Biology ,Asthma ,Mice, Knockout ,Interleukin-13 ,Lung ,medicine.diagnostic_test ,biology ,Tight junction ,business.industry ,Smoking ,respiratory system ,medicine.disease ,respiratory tract diseases ,Enzyme Activation ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,Bronchoalveolar lavage ,Endocrinology ,medicine.anatomical_structure ,Claudins ,Zonula Occludens-1 Protein ,biology.protein ,Respiratory epithelium ,Interleukin-4 ,Interleukin-5 ,business ,Bronchoalveolar Lavage Fluid ,030215 immunology - Abstract
The occurrence of asthma is closely related to environmental factors such as cigarette smoke (CS), one of the common risk factors. Environmental stimuli have the potential to activate transient receptor potential ankyrin 1 (TRPA1) and cause or aggravate asthma. The destruction of tight junctions (TJs) between airway epithelial cells by environmental stimuli in asthma has been researched. It is worth exploring whether CS can injury TJs and aggravate asthma by activating TRPA1. The objective of this study was to investigate the aggravation of CS on ovalbumin (OVA)-induced asthma related phenotypes and TJs expression in mice, and to explore the relationship between TRPA1 and the expression of TJs protein. Female wild type (WT) C57BL/6 mice, induced by OVA, CS and OVA plus CS (OVA + CS) respectively, were used to establish a 42-day asthma model, and mice with TRPA1 knockout (TRPA1−/−) were treated in the same way. This study detected the number of inflammatory cells in peripheral blood and bronchoalveolar lavage fluid (BALF), the levels of IL-4, IL-5, IL-13 in BALF, enhanced pause (Penh) of lung function, pathological changes and the gene and protein expressions of TRPA1 and TJs (including ZO-1, Occludin and Claudin-2) in lung tissues. Compared with normal saline (NS) group, WT mice in the OVA group and OVA + CS group were significantly higher in asthma related phenotypes. The WT-OVA + CS group also showed higher Penh value, levels of IL-5 and IL-13 in BALF and lung tissue injury scores when compared with the WT-OVA group and WT-CS group. However, WT-OVA + CS group mice had significantly larger number of neutrophils in BALF than the WT-OVA group, and had larger number of eosinophils in peripheral blood and higher levels of IL-4 in BALF than the WT-CS group. Meanwhile, compared with the WT-NS group, the expressions of TRPA1 and Claudin-2 in lung tissues increased in other three groups while their expressions of ZO-1 and Occludin decreased, among which, the WT-OVA + CS group showed more remarkable changes. Compared with the WT-OVA + CS group, mice in the TRPA1−/−-OVA + CS showed a significant decrease in the number of inflammatory cells, levels of IL-4, IL-5 and IL-13 in BALF, Penh value and lung tissue injury score, and a downregulation of Claudin-2 expression while an upregulation of ZO-1 and Occludin expressions. In addition, the airway inflammation and injury, and the expressions of ZO-1, Occluding and Claudin-2 expressions were found with no statistic differences between TRPA1−/−-OVA group and TRPA1−/−-OVA + CS group. These results suggest that CS has aggravated the airway inflammation, pathological damage and destruction of TJs in airway epithelium of OVA-induced asthmatic mice, the processes of which are related to the increase of TRPA1 expression.
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- 2021
42. Breast implant-associated ALCL (BIA-ALCL). Experience at Douglass Hanly Moir Pathology
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Leonard Hsu, Kwan Yee Tsu, Jenny Turner, Ivan Burchett, Anita Muljono, King Tan, Oana Crainic, Kathleen Young, Jessamine Reddy, Nirmala Pathmanathan, Suzanne Danieletto, Lisa Lin, Kathleen Merrick, Caroline Kurek, Eva Fong, Banu Banuthevan, Marcella Roman, Thomas Lynch, Alison Barnett, Caroline Edralin, Debbie Ekman, Frances Siganakis, Jasminka Humcevic, Jeffrey Ashdown, Joanne Crescini, Li Ping Zhou, Mario Rossetto, Reena Small, Ross McDonald, Shirley George, Sophie Zuo, and Cristina Vargas
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Pathology and Forensic Medicine - Published
- 2023
43. Effect of Houpo-Mahuang Decoction on aggravated asthma induced by cigarette smoke and the expression of TRPA1 and tight junctions in mice
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Yu-bo Sun, Ying Ni, Xin-sheng Fan, Li-ping Zhou, Qin-fei Yue, and Er-xin Shang
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Pharmacology ,Ankyrins ,Mice, Inbred BALB C ,Ovalbumin ,Asthma ,Cigarette Smoking ,Tight Junctions ,Mice, Inbred C57BL ,Disease Models, Animal ,Mice ,1-Butanol ,Transient Receptor Potential Channels ,Drug Discovery ,Animals ,Female ,Interleukin-4 ,Bronchoalveolar Lavage Fluid ,Lung ,TRPA1 Cation Channel ,Drugs, Chinese Herbal - Abstract
Cigarette smoke (CS) is a common environmental irritant and a risk factor for asthma, as it induces as well as aggravates asthmatic attacks. The injured airway epithelial tight junctions (TJs) aggravate asthma. CS can aggravate asthma by activating the transient receptor potential ankyrin A1 (TRPA1) channel and enhancing TJs destruction. Houpo Mahuang decoction (HPMHD) is a classic traditional Chinese prescription for the treatment of asthma. However, its underlying action mechanism is unclear.The present study aimed to evaluate the effect of HPMHD on the asthma phenotype and the regulation of TRPA1 and TJs in a CS-induced mouse model of aggravated asthma.Under optimized chromatographic and mass spectrometry conditions, the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) technique was used to detect and analyze the major chemical components of HPMHD. C57BL/6 female mice were randomly divided into seven groups, viz, normal saline (NS) group, ovalbumin (OVA) + CS group, dexamethasone group, HPMHD high-dose group and low-dose groups, n-butanol extract group, and ethyl acetate extract group, with 10 mice in each group. OVA sensitization and challenge, and CS exposure were used to establish the aggravated asthma model. As the main indices to evaluate the protective effect of HPMHD, the eosinophils count in peripheral blood, percentages of inflammatory cells classified and the levels of interleukin (IL)-4, IL-5, IL-13 in the bronchoalveolar lavage fluid (BALF), airway responsiveness enhanced pause (Penh), and changes in lung histopathology were determined and compared among the groups. The mRNA and protein expression of TRPA1 and TJs in lung tissue was also examined.Using UPLC-QTOF-MS, the chemical components of HPMHD, including ephedrine, pseudoephedrine, laetrile, and amygdalin amide, were identified by 51 signal peaks. Compared with those in the NS group, the eosinophil number in the peripheral blood and the eosinophils and neutrophils percentages in BALF of the OVA + CS group were remarkably increased. Following the inhalation of 50 μl of acetylcholine chloride (ACH) at doses of 25 and 50 mg/mL, the Penh increased significantly (p 0.01). Moreover, in the OVA + CS group, hematoxylin and eosin (HE) staining of lung tissue showed a significant number of infiltrated inflammatory cells, increased mucus secretion in the lumen, damaged bronchial mucosa, increased thickness of tracheal wall, and increased score of lung damage (p 0.01). The IL-4/5/13 levels were also remarkably increased (p 0.01). The protein as well as gene expression of both ZO-1 and occludin decreased markedly in the lung tissue, while the expression of TRPA1 and claudin-2 was increased (p 0.05, p 0.01). Next, the OVA + CS group and the treatment groups were compared. The inflammatory cells, Penh value, and levels of IL-4/5/13 were significantly reduced, and less lung injury was observed in the treatment groups. The gene and protein levels of TRPA1 and TJs were corrected (p 0.05, p 0.01); the effects on the HPMHD high-dose and ethyl acetate extract groups were particularly remarkable.HPMHD reduced airway hyperresponsiveness, inflammatory cell recruitment and Th2 cytokine secretion in CS-induced aggravated asthma mice, in a manner potentially dependent on regulation of the expression of TRPA1 and TJ proteins. Both the n-butanol and ethyl acetate extracts contained the active ingredients, especially the ethyl acetate extract.
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- 2022
44. Effect of Houpo-Mahuang Decoction on Aggravated Asthma Mice Model Induced by Cigarette Smoke and the Expressions of TRPA1 and Tight Junctions in Mice
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Yu-bo Sun, Ying Ni, Xinsheng Fan, Li-ping Zhou, and Qin-fei Yue
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- 2022
45. Efficacy of Stereotactic Body Radiation Therapy in Patients With Pd-1 Inhibitor–transarterial Chemoembolization Refractory Intermediate-stage Hepatocellular Carcinoma: a Retrospective Controlled Study
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Yu-Qiang Cheng, Wei-Jun Wang, Shuang Feng, Xiao-Wei Li, Yan-Jun Xiang, Shu-Qun Cheng, Li-Ping Zhou, Yan Meng, Hong-Ming Yu, Jian Zhai, Ying-Yi Qin, Kang Wang, Yi-Tao Zheng, Xi Cheng, Yun-Feng Shan, and Jin-Kai Feng
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medicine.medical_specialty ,biology ,business.industry ,Stereotactic body radiation therapy ,medicine.disease ,Intermediate stage ,Refractory ,Hepatocellular carcinoma ,Programmed cell death 1 ,medicine ,biology.protein ,In patient ,Radiology ,business - Abstract
Introduction: Transarterial chemoembolization (TACE) combined with PD-1 inhibitor is a novel and effective combination therapy. However, there is currently a gap in subsequent treatment when patients are diagnosed with PD-1 inhibitors-TACE refractoriness.Methods: This retrospective cohort study included enrolled patients with intermediate stage HCC who were diagnosed with PD-1 inhibitor-TACE refractoriness between January 2019 and December 2020 in the Eastern Hepatobiliary Surgery Hospital and the First Affiliated Hospital of Wenzhou Medical University. They were divided into two cohorts, (1) those who switched from TACE combined with PD-1 to stereotactic body radiation therapy (SBRT) combined with PD-1 and (2) those who continued TACE combined with PD-1. Progression free survival (PFS), overall survival (OS), and tumor response were assessed in both groups after refractory to PD-1 inhibitors combined with TACE.Results: Of the seventy-six patients included in this study, the median PFS was 19.6 months in the SBRT group (n=31) and 10.1 months in the TACE group (n=45, pConclusions: SBRT combined with PD-1 inhibitor improves PFS and OS in patients refractory to PD-1 inhibitor combined with TACE. Therefore, SBRT is recommended in case of failure of treatment with PD-1 inhibitors combined with TACE.
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- 2021
46. A nerve conduit filled with Wnt5a-loaded fibrin hydrogels promotes peripheral nerve regeneration
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Feng Rao, Dian-ying Zhang, Yi-jun Liu, Yanhua Wang, Li-ping Zhou, and Xiao-feng Chen
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Vascular Endothelial Growth Factor A ,Nerve guidance conduit ,Ciliary neurotrophic factor ,Fibrin ,Wnt-5a Protein ,nerve growth factor ,Rats, Sprague-Dawley ,Neurotrophic factors ,Peripheral Nerve Injuries ,Physiology (medical) ,nerve conduit ,Animals ,peripheral nerve injury ,Pharmacology (medical) ,Pharmacology ,biology ,vascular endothelial growth factor ,Chemistry ,Hydrogels ,Recovery of Function ,Original Articles ,Wnt5a ,Sciatic Nerve ,Cell biology ,Nerve Regeneration ,Rats ,body regions ,Psychiatry and Mental health ,Nerve growth factor ,Peripheral nerve injury ,embryonic structures ,biology.protein ,Original Article ,Sciatic nerve ,sense organs ,Schwann Cells ,Neurotrophin - Abstract
Aims Peripheral nerve injury is a significant clinical problem with a substantial impact on quality of life, for which no optimal treatment has been found. This study aimed to analyze the effect and mechanism of Wnt5a‐loaded fibrin hydrogel on a 10‐mm rat sciatic nerve defect. Methods The Wnt5a‐loaded fibrin hydrogel was synthesized by mixing a Wnt5a solution with thrombin and fibrinogen solutions. The loading capacity and release profile of Wnt5a‐loaded fibrin hydrogel and the effect of Wnt5a on Schwann cells were evaluated in vitro. We also assessed the in vivo repair status via histological analysis of the regenerative nerve and gastrocnemius muscle, electrophysiological examination, gait analysis, and muscle wet weight. Results We developed a nerve conduit filled with Wnt5a‐loaded fibrin hydrogel (Fn) as a sustained‐release system to repair a 10‐mm rat sciatic nerve defect. In vitro, Wnt5a could promote SC proliferation and the gene expression of vascular endothelial growth factor (VEGF), nerve growth factor (NGF), and cholinergic neurotrophic factor (CNTF), as well as the protein secretion of VEGF and NGF. In vivo, the Wnt5a/Fn group was superior to the hollow, fibrin hydrogel, and Wnt5a groups in terms of axonal growth, myelination, electrophysiological recovery, target organ innervation, and motor function recovery 12 weeks after the operation. Conclusion The Wnt5a/Fn nerve conduit can promote peripheral nerve defect regeneration, with potential clinical applications. The mechanism for this may be the facilitation of multiple neurotrophin secretion, combining vascularization and neurotrophic growth cues., The nerve conduit filled with Wnt5a‐loaded fibrin hydrogel as a sustained‐release system was developed to repair a 10‐mm rat sciatic nerve defect, which can promote peripheral nerve defect regeneration with potential in clinical application. The mechanism may be the facilitation of multiple neurotrophin secretion combining vascularization and neurotrophic growth cues.
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- 2021
47. Asiatic acid alleviates liver fibrosis via multiple signaling pathways based on integrated network pharmacology and lipidomics
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Yong, Li, Xu-Lin, He, Li-Ping, Zhou, Xiao-Zhong, Huang, Shan, Li, Su, Guan, Jing, Li, and Lei, Zhang
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Liver Cirrhosis ,Pharmacology ,Mice ,Arachidonic Acid ,Liver ,Lipidomics ,Hepatic Stellate Cells ,Animals ,Humans ,Network Pharmacology ,Pentacyclic Triterpenes ,Signal Transduction - Abstract
Liver fibrosis is characterized by the abnormal deposition of the extracellular matrix with a severe inflammatory response and/or metabolic disorder. Asiatic acid (AA), a natural compound derived from Centella asiatica, exhibited potent anti-fibrosis effects. This investigation first confirmed the anti-fibrosis effects of AA in TGF-β-LX-2 cells and CCl
- Published
- 2022
48. Two New Approaches for Multi-Attribute Group Decision-Making With Interval-Valued Neutrosophic Frank Aggregation Operators and Incomplete Weights
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Jiu-Ying Dong, Li-Ping Zhou, and Shu-Ping Wan
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Theoretical computer science ,General Computer Science ,General Engineering ,interval-valued neutrosophic set ,Interval valued ,Group decision-making ,interval-valued neutrosophic frank weighted averaging operator ,Multi-attribute group decision-making ,General Materials Science ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,Electrical and Electronic Engineering ,interval-valued neutrosophic frank weighted geometric operator ,lcsh:TK1-9971 ,Mathematics - Abstract
This paper investigates some Frank aggregation operators of interval-valued neutrosophic numbers (IVNNs) and applies to multi-attribute group decision-making (MAGDM) problems. First, the Frank t-conorm and t-norm are extended to interval-valued neutrosophic environment. Some new operational laws for IVNNs are defined and their related properties are investigated. Based on these new operational laws, some new aggregation operators for IVNNs are developed including the interval-valued neutrosophic Frank weighted averaging (IVNFWA) operator and the interval-valued neutrosophic Frank weighted geometric (IVNFWG) operator. Then some desirable properties and special cases of these new operators are further discussed. To solve the MAGDM with IVNNs, the weights of decision makers (DMs) are determined by using extended technique for order preference by similarity to ideal solution (TOPSIS) method based on cross-entropy. Additionally, attribute weights are determined based on the similarity degrees between alternatives and the absolute ideal solutions. Further, two new decision-making approaches for MAGDM with IVNNs are put forward by means of the IVNFWA and IVNFWG operators, respectively. Finally, a case study of selecting an agricultural socialization service provider is analyzed to illustrate the practicality and effectiveness of the developed two approaches.
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- 2019
49. Clinical Features of Aortic Dissection in the Emergency Department: A Single-center Experience from South China
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Xiang-Min Li, Guo-Qing Huang, Ai-min Wang, Li-Ping Zhou, Xiao-Ye Mo, and Fang-Jie Zhang
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Adult ,Male ,General Medicine ,Middle Aged ,Aortic Dissection ,Logistic Models ,Risk Factors ,Hypertension ,Emergency Medicine ,Humans ,Female ,Emergency Service, Hospital ,Aged ,Retrospective Studies - Abstract
Objectives: Our goal in this study was to determine 1) whether there are any differences in clinical characteristics between Chinese and Western patients with aortic dissection (AD), and 2) the mortality rate of AD patients in the emergency department (ED) and identify the risk predictors for death. Methods: We retrospectively analyzed patients who were diagnosed with AD and admitted to our ED between September 1, 2017–August 31, 2020. Data on age, gender, clinical manifestation, medical history, routine blood tests, liver and kidney function, coagulation, myocardial enzymology, and mortality were collected. Results: We enrolled 535 AD patients (422 men and 113 women) with a mean age of 54.7±14.1 years. Type A AD constituted 40% of the total number of AD cases, while type B AD constituted 60%. The proportion of those who were females, 10-92 years, with type A AD, and hypertension in the Chinese population was lower than that in the Western population (P
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- 2021
50. Automatic keyword extraction from documents based on multiple content-based measures.
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Kun Yue, Wei-Yi Liu, and Li-Ping Zhou
- Published
- 2011
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