389 results on '"Li-Hong Wang"'
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2. Causal association between major depressive disorder and venous thromboembolism: a bidirectional mendelian randomization study
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Hong-Yan Li, Li-Hong Wang, Jing Wang, Yong-Bo Wang, and Hai-Shan Wang
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major depressive disorder ,venous thromboembolism ,risk factor ,singlenucleotide polymorphisms ,mendelian randomization ,Genetics ,QH426-470 - Abstract
PurposeMajor depressive disorder (MDD) and venous thromboembolism (VTE) may be linked in observational studies. However, the causal association remains ambiguous. Therefore, this study investigates the causal associations between them.MethodsWe performed a two-sample univariable and multivariable bidirectional Mendelian randomization (MR) analysis to evaluate the associations between MDD and VTE. The summary genetic associations of MDD statistics were obtained from the Psychiatric Genomics Consortium and UK Biobank. Information on VTE, deep vein thrombosis (DVT), and pulmonary embolism (PE) were obtained from the FinnGen Biobank. Inverse-variance weighting was used as the main analysis method. Other methods include weighted median, MR-Egger, Simple mode, and Weighted mode.ResultsUnivariable MR analysis revealed no significant associations between MDD and VTE risk (odds ratio (OR): 0.936, 95% confidence interval (CI): 0.736–1.190, p = 0.590); however, after adjusting the potential relevant polymorphisms of body mass index and education, the multivariable MR analysis showed suggestive evidence of association between them (OR: 1.163, 95% CI: 1.004–1.346, p = 0.044). Univariable MR analysis also revealed significant associations between MDD and PE risk (OR: 1.310, 95% CI: 1.073–1.598, p = 0.008), but the association between them was no longer significant in MVMR analysis (p = 0.072). We found no significant causal effects between MDD and DVT risk in univariable or multivariable MR analyses. There was also no clear evidence showing the causal effects between VTE, PE, or DVT and MDD risk.ConclusionWe provide suggestive genetic evidence to support the causal association between MDD and VTE risk. No causal associations were observed between VTE, PE, or DVT and MDD risk. Further validation of these associations and investigations of potential mechanisms are required.
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- 2024
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3. The E3 ubiquitin ligase HUWE1 acts through the N‐Myc‐DLL1‐NOTCH1 signaling axis to suppress glioblastoma progression
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Ye Yuan, Li‐Hong Wang, Xian‐Xian Zhao, Jiao Wang, Meng‐Si Zhang, Qing‐Hua Ma, Sen Wei, Ze‐Xuan Yan, Yue Cheng, Xiao‐Qing Chen, Hong‐Bo Zou, Jia Ge, Yan Wang, Xia Zhang, You‐Hong Cui, Tao Luo, and Xiu‐Wu Bian
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DLL1 ,E3 ubiquitin ligase ,glioblastoma ,HUWE1 ,N‐Myc ,NOTCH1 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Elucidation of the post‐transcriptional modification has led to novel strategies to treat intractable tumors, especially glioblastoma (GBM). The ubiquitin‐proteasome system (UPS) mediates a reversible, stringent and stepwise post‐translational modification which is closely associated with malignant processes of GBM. To this end, developing novel therapeutic approaches to target the UPS may contribute to the treatment of this disease. This study aimed to screen the vital and aberrantly regulated component of the UPS in GBM. Based on the molecular identification, functional characterization, and mechanism investigation, we sought to elaborate a novel therapeutic strategy to target this vital factor to combat GBM. Methods We combined glioma datasets and human patient samples to screen and identify aberrantly regulated E3 ubiquitin ligase. Multidimensional database analysis and molecular and functional experiments in vivo and in vitro were used to evaluate the roles of HECT, UBA and WWE domain‐containing E3 ubiquitin ligase 1 (HUWE1) in GBM. dCas9 synergistic activation mediator system and recombinant adeno‐associated virus (rAAV) were used to endogenously overexpress full‐length HUWE1 in vitro and in glioma orthotopic xenografts. Results Low expression of HUWE1 was closely associated with worse prognosis of GBM patients. The ubiquitination and subsequent degradation of N‐Myc mediated by HUWE1, leading to the inactivation of downstream Delta‐like 1 (DLL1)‐NOTCH1 signaling pathways, inhibited the proliferation, invasion, and migration of GBM cells in vitro and in vivo. A rAAV dual‐vector system for packaging and delivery of dCas9‐VP64 was used to augment endogenous HUWE1 expression in vivo and showed an antitumor activity in glioma orthotopic xenografts. Conclusions The E3 ubiquitin ligase HUWE1 acts through the N‐Myc‐DLL1‐NOTCH1 signaling axis to suppress GBM progression. Antitumor activity of rAAV dual‐vector delivering dCas9‐HUWE1 system uncovers a promising therapeutic strategy for GBM.
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- 2022
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4. Factors associated with left ventricular diastolic dysfunction in patients with septic shock
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Wei-Dong Ge, Feng-Zhi Li, Bang-Chuan Hu, Li-Hong Wang, and Ding-Yuan Ren
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Sepsis, Septic shock ,Left ventricular diastolic dysfunction ,Ultrasound ,Risk factor ,Medicine - Abstract
Abstract Purpose To investigate risk factors associated with left ventricular diastolic dysfunction (LVDD) of patients with septic shock. Materials and methods Patients with septic shock concomitant with or without LVDD were retrospectively enrolled and divided into the LVDD group (n = 17) and control without LVDD (n = 85). The clinical and ultrasound data were analyzed. Results A significant (P 37 mL/m2, septal e’ 15 (OR 22.86, 95% CI 6.09–85.79), lateral e’ 13 (OR 52, 95% CI 11.99- 225.55), lateral s’ 10, E/average e’ > 10 (OR 9.53, 95% CI 2.49–36.46), APACHE II score > 16 (OR 3.33, 95% CI 1.00–11.03), SOFA > 5 (or 3.43, 95% CI 1.11–10.60), BUN > 12 mmol/L (OR 3.37, 95% CI 1.15–9.87), serum creatinine > 146 μmol/L (OR 5.08, 95% CI 1.69–15.23), serum glucose > 8 mmol/L (OR 3.36, 95% CI 1.09–10.40), and triglyceride > 1.8 mmol/L were significant (P 37 ml/m2, lateral e’ 13, and SOFA > 5 were significant (P
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- 2022
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5. Corrigendum: Anesthesia management of patients undergoing off-pump coronary artery bypass grafting: A retrospective study of single center
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Yong Lin, Tao Sun, Ning-ning Cheng, Jing-jing Liu, Li-xian He, Li-hong Wang, Xian-wen Liu, Mei-fang Chen, Liang-wan Chen, Yun-tai Yao, and the Evidence in Cardiovascular Anesthesia (EICA) Group
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coronary artery bypass ,anesthesia management ,single center ,retrospective ,hemodynamics ,Surgery ,RD1-811 - Published
- 2023
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6. Ezrin expression in female reproductive tissues: A review of regulation and pathophysiological implications
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Wen-Ting Xu, Ling-Li Shi, Jie Xu, Haiqing Qian, Huifang Zhou, and Li-Hong Wang
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ezrin ,female reproductive ,embryo ,oocytes ,follicle ,Biology (General) ,QH301-705.5 - Abstract
Ezrin, a plasma membrane-microfilament linker, is a cytoskeletal organizer involved in many cellular activities by binding to the membrane protein-ezrin-cytoskeletal protein complex and regulating downstream signal transduction. Increasing evidence demonstrates that ezrin plays an important role in regulating cell polarity, proliferation and invasion. In this study, we analyzed the effects of ezrin on oocytes, follicle development, embryo development and embryo implantation. We reviewed the recent studies on the modalities of ezrin regulation and its involvement in the biological processes of female reproductive physiology and summarized the current research advances in ezrin inhibitors. These studies will provide new strategies and insights for the treatment of diseases.
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- 2023
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7. Anesthesia management of patients undergoing off-pump coronary artery bypass grafting: A retrospective study of single center
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Yong Lin, Tao Sun, Ning-ning Cheng, Jing-jing Liu, Li-xian He, Li-hong Wang, Xian-wen Liu, Mei-fang Chen, Liang-wan Chen, Yun-tai Yao, and the Evidence in Cardiovascular Anesthesia (EICA) Group
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coronary artery bypass ,anesthesia management ,single center ,retrospective ,hemodynamics ,Surgery ,RD1-811 - Abstract
BackgroundTo summarize the current practice of anesthesia management for Chinese patients undergoing off-pump coronary artery bypass (OPCAB) surgery at a large-volume cardiovascular center.Materials and methodsThe clinical data of consecutive patients undergoing isolated, primary OPCAB surgery during the period from September 2019 to December 2019 were retrospectively analyzed. Patient characteristics, intraoperative data, and short-term outcomes were extracted from the Hospital Information System and the Anesthesia Information Management System.ResultsA total of 255 patients who underwent OPCAB surgery were enrolled in the current study. High-dose opioids and short-acting sedatives were the most commonly administrated anesthetics intraoperatively. Pulmonary arterial catheter insertion is frequently performed in patients with serious coronary heart disease. Goal-directed fluid therapy, a restricted transfusion strategy, and perioperative blood management were routinely used. Rational usages of inotropic and vasoactive agents facilitate hemodynamic stability during the coronary anastomosis procedure. Four patients underwent re-exploration for bleeding, but no death was observed.ConclusionsThe study introduced the current practice of anesthesia management at the large-volume cardiovascular center, and the short-term outcomes indicated the efficacy and safety of the practice in OPCAB surgery.
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- 2023
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8. Elution and Biomechanical Properties of Meropenem‐Loaded Bone Cement
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Li‐hong Wang, Ya‐dong Feng, Xiao‐wei Zhang, Long Jin, Fang‐lun Zhou, and Guo‐hong Xu
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Biomechanical phenomena ,Bone cements ,Kinetics ,Meropenem ,Vancomycin ,Orthopedic surgery ,RD701-811 - Abstract
Objective To investigate the biomechanical and elution properties of meropenem‐loaded bone cement. Methods Bone cement (Palacos LV) with 5% (2 g/4 0g), 10% (4 g/40 g), and 15% (6 g/40 g) meropenem; 5% (2 g/40 g) and 10% (4 g/40 g) vancomycin; and blank bone cement were prepared in a total of six groups named A2, A4, A6, B2, B4, and A0 (antibiotic‐free). 36 cylinder specimens (6‐mm diameter and 12‐mm height) of all six groups were molded for a compression test. After the compression test, because of mechanical properties below the ISO standard requirements, groups B2, B4 were not subjected to a bending test. So a total of 24 rectangular strip specimens (10‐mm width, 75‐mm length, and 3.3‐mm thickness) for groups A2, A4, A6 and A0 were molded for the bending test. Between‐group differences of compressive strength, bending strength and bending modulus were analyzed. The meropenem standard was prepared as a series of standard solutions to calculate the standard curve. At a constant temperature of 37 °C, separately, meropenem‐loaded bone cement cylinder specimens (12 mm in diameter and 17 mm in length) of A2, A4 and A6 were serially immersed in saline solution without stirring. The eluent drug concentration at 24, 48, 72 h and 6, 12, 24 days was measured and the drug concentration‐time curve of meropenem was constructed. Results With the exception of groups B2 and B4, all cements compressive strength values were well above the minimum requirement of the ISO 5833 standard (70 MPa). The compressive strength and bending strength values of group A4 were higher than those of group A0 (P 0.05). There were no intergroup differences of bending modulus between the A0, A2, A4 and A6 groups (P > 0.05). A standard curve of meropenem was obtained and a regression equation was constructed: Y = 15.0265 X + 13.5218, r = 1.00. At 37 °C, the release of meropenem was rapid during the first 48 h for all A2, A4, A6 samples, and subsequent release continued to decrease. Conclusion When adding up to 15% (6 g/40 g) meropenem to the bone cement, the biomechanical properties were not reduced, and bone cement with 10% (4 g/40 g) meropenem had the best performance. At a constant temperature of 37°C, meropenem can be released from bone cement for up to 24 days.
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- 2021
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9. Lifestyle-related risk factors correlated with mental health problems: A longitudinal observational study among 686 male college students in Chongqing, China
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Bin-Wei Yang, Peng Zou, Qing Chen, Lei Sun, Xi Ling, Huan Yang, Ni-Ya Zhou, Li-Hong Wang, Lin-Ping Huang, Jin-Yi Liu, Hui-Fang Yang, Jia Cao, and Lin Ao
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lifestyle factors ,mental health problems ,DASS-21 ,college students ,longitudinal observational study ,Public aspects of medicine ,RA1-1270 - Abstract
AimPublic concerns over the mental health problems of college students are rising. Previous research show that female tend to suffer more from mental health problems than males, with few studies focusing on males. This study sought to explore the association of lifestyle-related risk factors with the prevalence of mental health problems among male college students in China.MethodsThe lifestyle information and mental health status of 686 male college students from Chongqing, China, were assessed in 2014, and 582 of them were followed up a year later. Participants completed a questionnaire assessing demographic and lifestyle factors which include sleep quality, computer usage, sedentariness, physical activity, smoking, current alcohol, coke, coffee, and milk tea drinking, and current tea/fried food/baked food consumption. Mental health problems were measured using the Depression Anxiety Stress Scale-21 (DASS-21).ResultsUnivariate analyses indicated that age, sleep latency, sleep duration, computer usage time, milk tea drinking, and fried food consumption were potential risk factors for mental health problems (p's < 0.05). Multivariate analysis further revealed that, either at baseline or during follow-up, participants with (i) more computer usage time were at a higher risk of having depression symptoms (p's < 0.05) and (ii) a higher frequency of fried food consumption were associated with a higher risk of having depression, anxiety, and stress symptoms (p's < 0.05). Additionally, the cross-lagged analysis showed that (i) computer usage time in 2014 is positively correlated with depression status (β = 0.106, p < 0.05) but not anxiety (β = 0.047, p > 0.05) and stress (β = 0.019, p > 0.05) status a year later and (ii) fried food consumption in 2014 is positively correlated with depression (β = 0.129, p < 0.01), anxiety (β = 0.168, p < 0.001), and stress (β = 0.113, p < 0.01) status a year later.ConclusionsComputer usage time and fried food consumption were lifestyle-related risk factors for mental health problems in male college students in Chongqing, China. These results might emphasize further preventive strategies for mental health problems, especially in male college students.
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- 2022
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10. Corrigendum: Regulation of microrna-497-targeting AKT2 influences tumor growth and chemoresistance to cisplatin in lung cancer
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Lin Wang, Xiang-Bo Ji, Li-Hong Wang, Jian-Ge Qiu, Feng-Mei Zhou, Wen-Jing Liu, Di-di Wan, Marie Chai-mi Lin, Ling-Zhi Liu, Jian-Ying Zhang, and Bing-Hua Jiang
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MiR-497 ,Akt2 ,tumor growth ,chemoresistance ,NSCLC ,Biology (General) ,QH301-705.5 - Published
- 2022
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11. Risk Factors and Outcomes for Preoperative Asymptomatic Pulmonary Embolism in Patients Aged 60 Years and Over with Hip Fracture
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Fang‐lun Zhou, Li‐hong Wang, Cheng‐qian Dai, Guo‐jian Shentu, and Guo‐hong Xu
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Hip fractures ,Preoperative ,Pulmonary embolism ,Orthopedic surgery ,RD701-811 - Abstract
Objective To investigate the risk factors for, and outcomes of, preoperative asymptomatic pulmonary embolism (PE) in patients ≥60 years old following delayed operation for hip fracture. Methods From March 2017 to December 2018, 90 patients aged ≥60 years with hip fracture who suffered a delay in surgery were recruited to this prospective study following admission to our hospital. Computed tomography pulmonary angiography (CTPA) was used to detect preoperative asymptomatic PE and calculated its incidence. Time from injury to admission, baseline characteristics, medical comorbidities, and blood biomarker levels were evaluated as potential risk factors. Logistic regression analysis was used to identify risk factors. Mortality and major bleeding events were recorded and compared between individuals with PE and without. Data were analyzed by t‐test, Mann–Whitney U test, χ2 test, Fisher's exact test, and logistic regression analysis. Results The incidence of preoperative asymptomatic PE was 18.9% (17/90 patients). In the univariate analysis, the risk factors for preoperative asymptomatic PE were male sex, hypertension, cerebrovascular accident, smoking, plasma D‐dimer level, potassium level, urea level, creatinine level, and cysteine level. Multivariate logistic regression analysis showed that the risk of preoperative asymptomatic PE was higher in patients with hypertension (odds ratio [OR] = 10.048; 95% confidence interval [CI], 1.118–90.333), cerebrovascular accident (OR = 20.135; 95% CI, 1.875–216.164), smoking (OR = 48.741; 95% CI, 4.155–571.788), high plasma D‐dimer levels (OR = 1.200; 95% CI, 1.062–157.300), and high plasma potassium levels (OR = 12.928; 95% CI, 1.062–157.300). All patients were followed up for 21.0 months (range, 2 to 36 months). Mortality within the first year postoperatively was higher in patients with PE (29.41% vs 9.59%, P = 0.046). Conclusions In view of the high incidence of preoperative asymptomatic PE and the inferior prognosis in individuals with PE, routine CTPA examination for preoperative asymptomatic PE could be useful for patients aged ≥60 years with hip fracture for whom surgery is delayed.
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- 2021
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12. Association of thyroid hormones with blood pressure and arterial stiffness in the general population: The Dali study
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Md Tasneem Jamal, Qing‐Lu Li, Qi‐Yan Li, Wan‐Ying Liang, Li‐Hong Wang, Jian‐Hang Wei, Quan Liang, Nai‐Qing Hu, and Li‐Hua Li
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arterial stiffness ,blood pressure ,Chinese ,population ,thyroid hormone ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Thyroid dysfunction plays a role in blood pressure (BP) regulation. However, the associations between thyroid function and BP and arterial stiffness in the general Chinese population without thyroid disease are unknown. This population‐based cross‐sectional study aimed to investigate the association between thyroid function and peripheral and central BP and arterial stiffness in Chinese individuals. After excluding those who had thyroid diseases or incomplete clinical measurements, this study included 691 participants. Of the participants, 444 (64.2%) were women and 215 (31.1%) had hypertension. After adjustment for covariates, serum FT3 was significantly associated with a higher pulse rate in both sexes. In men, each 2.72‐fold increase in serum FT4 levels was associated with higher peripheral systolic BP (+10.82 mmHg, p = .005) and pulse pressure (+5.71 mmHg, p = .03). Each 2.72‐fold increase in serum FT4 levels was associated with higher central systolic BP (+8.03 mmHg, p = .03) and pulse pressure (+3.89 mmHg, p = .05). In women, serum FT4 was only associated with a higher central pulse pressure (+2.96 mmHg, p = .04). After adjustment for covariates, serum FT4 was significantly associated with a faster cfPWV exclusively in men. Our study showed that serum FT4 is associated with higher peripheral and central BP and faster cfPWV in men, whereas serum FT3 is positively associated with a higher pulse rate in both sexes, indicating that the effects of thyroid function on BP and arterial stiffness are more significant in men than in women.
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- 2021
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13. No significant association of serum klotho concentration with blood pressure and pulse wave velocity in a Chinese population
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Wan-Ying Liang, Li-Hong Wang, Jian-Hang Wei, Qing-Lu Li, Qi-Yan Li, Quan Liang, Nai-Qing Hu, and Li-Hua Li
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Medicine ,Science - Abstract
Abstract Klotho, an important anti-aging protein, may be related to elevated blood pressure (BP) and arterial stiffness. We aimed to investigate associations between the serum klotho concentration and peripheral/central BP and arterial stiffness based on the carotid–femoral pulse wave velocity (cfPWV) in a Chinese population. We invited all inhabitants aged ≥ 18 years in two Dali communities for participation. The SphygmoCor system was used to record radial arterial waveforms. Aortic waveforms were derived using a generalized transfer function. The central BP was assessed by calibrating the brachial BP, which was measured using an oscillometric device. The serum klotho concentration was measured using an enzyme-linked immunosorbent assay and logarithmically transformed. Of the 716 participants (mean age: 51.9 ± 12.6 years), 467 (65.2%) were women. The median serum klotho concentration was 381.8 pg/mL. The serum klotho concentration did not significantly differ between patients with and without hypertension (P > 0.05) and between those with and without arterial stiffness (cfPWV ≥ 10 m/s) (P > 0.05). After adjusting for confounders, the serum klotho concentration was not significantly associated with the peripheral or central BP (P > 0.05) and cfPWV (P > 0.05). Our data indicated that the serum klotho concentration was not associated with BP or cfPWV in the general Chinese population.
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- 2021
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14. Supercapsular percutaneously assisted total hip arthroplasty versus conventional posterior approach: Comparison of early functional results
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Heng Jiang, Li-Hong Wang, Yong-Xin Jin, Zhi-Ming Liu, Liang-Feng Xu, and Xian-Yun Chen
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Orthopedic surgery ,RD701-811 - Published
- 2020
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15. Body Mass Index: An Effective Predictor of Ejection Fraction Improvement in Heart Failure
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Li-fang Ye, Xue-ling Li, Shao-mei Wang, Yun-fan Wang, Ya-ru Zheng, and Li-hong Wang
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body mass index ,obesity ,left ventricular ejection fraction ,improvement ,heart failure ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background: Heart failure patients with higher body mass index (BMI) exhibit better clinical outcomes. Therefore, we assessed whether the BMI can predict left ventricular ejection fraction (EF) improvement following heart failure.Methods and Results: We included 184 patients newly diagnosed with dilated cardiomyopathy and reduced EF in our center and who underwent follow-up examination of EF via echocardiography after 6 months. The EF improved at 6 months in 88 participants, who were included in the heart failure with recovered EF (HFrecEF) subgroup. Patients in whom the EF remained reduced were included in the heart failure with persistently reduced EF (persistent HFrEF) subgroup. Our analyses revealed that EF increase correlated with age (r = −0.254, P = 0.001), left ventricular diastolic dimension (LVDD; r = −0.210, P = 0.004), diabetes (P = 0.034), brain natriuretic peptide (r = −0.199, P = 0.007), and BMI grade (P = 0.000). BMI grade was significantly associated with elevated EF after adjustment for other variables (P = 0.001). On multivariable analysis, compared to patients with persistent HFrEF, those with HFrecEF had higher BMI [odds ratio (OR) = 2.342 per one standard deviation increase; P = 0.001] and lower LVDD (OR = 0.466 per one standard deviation increase; P = 0.001). ROC-curve analysis data showed that BMI > 22.66 kg/m2 (sensitivity 84.1%, specificity 59.4%, AUC 0.745, P = 0.000) indicate high probability of EF recovery in 6 months.Conclusions: Our data suggest that higher BMI is strongly correlated with the recovered EF and that BMI is an effective predictor of EF improvement in patients with heart failure and reduced EF.
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- 2021
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16. The changes in Bispectral index during recovery period in tracheal intubation general Anesthesia with or without tracheal-laryngopharynx topical Anesthesia
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Run-qiao Fu, Mian-rong Xue, Li-hong Wang, and Wang-yan Yang
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Surgery ,RD1-811 - Published
- 2022
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17. Regulation of MicroRNA-497-Targeting AKT2 Influences Tumor Growth and Chemoresistance to Cisplatin in Lung Cancer
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Lin Wang, Xiang-Bo Ji, Li-Hong Wang, Jian-Ge Qiu, Feng-Mei Zhou, Wen-Jing Liu, Di-di Wan, Marie Chai-mi Lin, Ling-Zhi Liu, Jian-Ying Zhang, and Bing-Hua Jiang
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miR-497 ,AKT2 ,tumor growth ,chemoresistance ,NSCLC ,Biology (General) ,QH301-705.5 - Abstract
BackgroundMicroRNA-497 (miR-497) has been implicated in several cancers. Increasing studies demonstrate the role of AKT2 in cancers as an oncogene which is closely associated with tumor aggressiveness by enhancing cancer cell survival, migration and invasion However, miR-497/AKT2 axis in non-small cell lung cancer (NSCLC) remains unclear.MethodsQuantitative real-time PCR (qRT-PCR) was used to quantify the expression of miR-497 and its target gene. The function of miR-497 in lung cancer was investigated through in vitro and in vivo assays (cell proliferation assay, cell migration assay, colony formation assay, flow cytometry assay, immunoblotting and tumorigenesis assay). Luciferase reporter assay was conducted to confirm the target gene of miR-497.ResultsIn this study, we found that miR-497 was significantly downregulated in tumor tissues and blood samples of lung cancer patients. To understand the potential mechanism of miR-497 in inhibiting tumor growth, we showed that miR-497 blocked the activation of AKT2 and regulated cell proliferation, cell migration, colony formation and increases chemosensitivity of H1299 cells to cisplatin by inhibiting AKT2. MiR-497 also inhibited tumor growth and suppressed expression of AKT2 at the protein and mRNA levels in mouse xenograft tumors.ConclusionTaken together, our findings indicated that miR-497 suppresses the tumor growth by targeting AKT2, and the miR-497/AKT2 axis is a potential therapeutic target for NSCLC intervention.
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- 2020
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18. Efficacy and Safety of Exercise Rehabilitation for Heart Failure Patients With Cardiac Resynchronization Therapy: A Systematic Review and Meta-Analysis
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Li-fang Ye, Shao-mei Wang, and Li-hong Wang
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cardiac rehabilitation ,heart failure ,cardiac resynchronization therapy ,meta-analysis ,randomized controlled trial ,Physiology ,QP1-981 - Abstract
Background: Many heart failure (HF) patients admitted to cardiac rehabilitation (CR) centers have a cardiac resynchronization therapy (CRT) device. However, information about the efficacy and safety of exercise rehabilitation in HF patients with a CRT device is scant. We assessed the effects of exercise rehabilitation in HF patients with a CRT device.Methods and Results: The PubMed, EMBASE, Cochrane Central Register of Controlled Trials, CINAHL, PsycInfo, China Biology Medicine, Wanfang, and China National Knowledge Infrastructure databases were searched comprehensively to identify randomized controlled trials (RCTs) published between January 1, 1990 and July 1, 2019 on exercise rehabilitation in HF patients with CRT devices. We identified seven studies published from 2006 to 2019, including 661 patients with an intervention duration of 8 to 24 weeks. Three studies reported all-cause mortality and serious adverse events, and no significant difference was found between exercise rehabilitation patients and controls at the longest available follow-up (both P > 0.05; both I2 = 0%). Exercise rehabilitation patients exhibited a higher exercise capacity (peak oxygen uptake: random-effect WMD = 2.02 ml/kg/min, 95% CI 0.62 to 3.41, P = 0.005, I2 = 67.4%; exercise duration: fixed-effect WMD = 102.34s, 95% CI 67.06 to 137.62, P < 0.001, I2 = 25%) after intervention, despite the significant heterogeneity of studies. Left ventricular ejection fraction (LVEF) was significantly improved in exercise rehabilitation patients compared to that in controls (fixed-effect WMD = 3.89%, 95% CI 1.50 to 6.28; P = 0.001; I2 = 48.0%). Due to differences in health-related quality of life (HRQOL) assessment methods, we only pooled data that reported Minnesota Living with Heart Failure Questionnaire scores. Exercise rehabilitation patients exhibited a better HRQOL than controls (fixed-effect WMD = −5.34, 95% CI −10.12 to −0.56; P = 0.028; I2 = 0%).Conclusions: Exercise rehabilitation may restore exercise capacity and cardiac function in HF patients with a CRT device. Furthermore, exercise training was associated with better HRQOL on follow-up.
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- 2020
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19. Valproic Acid Increased Autophagic Flux in human Multiple Myeloma Cells in Vitro
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Yang Wang, Chang-Lai Hao, Zhi-Hua Zhang, Li-Hong Wang, Li-Na Yan, Rong-Juan Zhang, Li Lin, and Yue Yang
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multiple myeloma ,valproic acid ,autophagic flux ,LC3-II ,autophagy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: To investigate the effects of valproic acid (VPA) on autophagic flux in multiple myeloma (MM) cells. Methods and Results: Cell proliferation was assayed by the Cell Counting Kit-8 assay. The qRT-PCR was used to measure the expressions of LC3-II at mRNA level. Autophagic flux was measured by LC3-II turnover using western blot analysis and flow cytometry using the fluorescent dye Cyto-ID. An assay using the RFP-GFP-LC3 tandem construct was performed to monitor autophagic flux. Cell proliferation assay showed that VPA could inhibit the proliferation of MM cells and the inhibitory effects were enhanced with the extension of time. The qRT-PCR and western blot showed that the expression level of LC3-II in the VPA plus CQ group was significantly higher than that in CQ group. Cyto-ID autophagy test showed that the intracellular average fluorescence intensity in VPA plus CQ group was significantly higher than that in control and VPA group (all p
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- 2020
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20. Shenmai Injection Improves Energy Metabolism in Patients With Heart Failure: A Randomized Controlled Trial
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Shao-mei Wang, Li-fang Ye, and Li-hong Wang
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heart failure ,energy metabolism ,Shenmai injection ,cardiac function ,traditional Chinese medicine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
BackgroundIn recent years, the application of Shenmai (SM) injection, a traditional Chinese medicine (TCM), to treat heart failure (HF) has been gradually accepted in China. However, whether SM improves energy metabolism in patients with HF has not been determined due to the lack of high-quality studies. We aimed to investigate the influence of SM on energy metabolism in patients with HF.MethodsThis single-blind, controlled study randomly assigned 120 eligible patients equally into three groups receiving SM, trimetazidine (TMZ), or control in addition to standard medical treatment for HF for 7 days. The primary endpoints were changes in free fatty acids (FFAs), glucose, lactic acid (LA), pyroracemic acid (pyruvate, PA) and branched chain amino acids (BCAAs) in serum. The secondary outcomes included the New York Heart Association (NYHA) functional classification, TCM syndrome score (TCM-s), left ventricular injection fraction (LVEF), left ventricular internal diastolic diameter (LVIDd), left ventricular internal dimension systole (LVIDs), and B-type natriuretic peptide (BNP).ResultsAfter treatment for 1 week, the NYHA functional classification, TCM-s, and BNP level gradually decreased in the patients in all three groups, but these metrics were significantly increased in the patients in the SM group compared with those in the patients in the TMZ and control groups (P < 0.05). Moreover, energy metabolism was improved in the NYHA III–IV patients in the SM group compared with those in the patients in the TMZ and control groups as evidenced by changes in the serum levels of FFA, LA, PA, and BCAA.ConclusionsIntegrative treatment with SM in addition to standard medical treatment for HF was associated with improved cardiac function compared to standard medical treatment alone. The benefit of SM in HF may be related to an improvement in energy metabolism, which seems to be more remarkable than that following treatment with TMZ.
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- 2020
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21. Dehiscence method: a seed-saving, quick and simple viability assessment in rice
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Ling-xiang Xu, Yi-xin Lin, Li-hong Wang, and Yuan-chang Zhou
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Ageing ,Dehydration ,Deterioration ,Dormancy ,Drought resistance ,Endangered species ,Plant culture ,SB1-1110 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Seed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion. Results Dehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds. Conclusion We demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species.
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- 2018
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22. MiR-199a Inhibits Tumor Growth and Attenuates Chemoresistance by Targeting K-RAS via AKT and ERK Signalings
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Wei Li, Lin Wang, Xiang-Bo Ji, Li-Hong Wang, Xin Ge, Wei-Tao Liu, Ling Chen, Zhong Zheng, Zhu-Mei Shi, Ling-Zhi Liu, Marie C. Lin, Jie-Yu Chen, and Bing-Hua Jiang
- Subjects
miR-199a ,glioma ,K-RAS ,chemoresistance ,tumorigenesis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Glioma is the most malignant brain tumors in the world, the function and molecular mechanism of microRNA-199a (miR-199a) in glioma is not fully understood. Our research aims to investigate miR-199a/K-RAS axis in regulation of glioma tumor growth and chemoresistance. The function of miR-199a in glioma was investigated through in vitro and in vivo assays. We found that miR-199a in tumor tissues of glioma patients was significantly downregulated in this study. Kinase suppressor of ras 1 (K-RAS), was indicated as a direct target of miR-199a, as well as expression levels of K-RAS were inversely correlated with expression levels of miR-199a in human glioma specimens. Forced expression of miR-199a suppressed AKT and ERK activation, decreased HIF-1α and VEGF expression, inhibited cell proliferation and cell migration, forced expression of K-RAS restored the inhibitory effect of miR-199a on cell proliferation and cell migration. Moreover, miR-199a renders tumor cells more sensitive to temozolomide (TMZ) via targeting K-RAS. In vivo experiment validated that miR-199a functioned as a tumor suppressor, inhibited tumor growth by targeting K-RAS and suppressed activation of AKT, ERK and HIF-1α expression. Taken together, these findings indicated that miR-199a inhibits tumor growth and chemoresistance by regulating K-RAS, and the miR-199a/K-RAS axis is a potential therapeutic target for clinical intervention in glioma.
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- 2019
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23. Clinical Analysis of Polyethylene Glycol Interferon-α Treatment in 155 Hepatitis B e Antigen (HBeAg)-Positive Chronic Hepatitis B (CHB) Patients
- Author
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Xin Luo, Ji-Xian Yu, Lei Xie, Wen-Jun Ma, and Li-Hong Wang
- Subjects
CHB ,Antiviral effect ,PEG interferon-α ,Specialties of internal medicine ,RC581-951 - Abstract
Purpose: This study aims to investigate the antiviral effect of polyethylene glycol (PEG)-interferon α-2a and PEG-interferon α-2b treatment on hepatitis B e antigen (HBeAg)-positive chronic hepatitis B (CHB) at the 48th week of treatment and the 24th and 48th week after withdrawal, in order to provide guidance on the antiviral treatment of HBeAg-positive CHB patients. Material and methods: Antiviral treatment was performed on 155 HBeAg-positive CHB patients. Among these patients, 66 patients received PEG-interferon α-2a treatment and 89 patients received PEG-interferon α-2b treatment; and these treatments were administered by subcutaneous injection, once per week, which lasted for 48 weeks. Other antiviral and hepatoprotective drugs were not used during the treatment. Results: At the 48th week of treatment, ALT recovery rate, HBsAg seroconversion rate, HBeAg seroconversion rate and HBV DNA titers dropped below 200 IU/mL rate were 69.7%, 6.1%, 27.3% and 50.0%, respectively, in the PEG-interferon α-2a group; and were 70.8%, 6.7%, 33.7% and 62.9%, respectively, in the PEG-interferon α-2b group. At the 24th and 48th week of follow-up after withdrawal, HBsAg seroconversion rate in these two groups did not change; and HBeAg seroconversion rate further increased. Furthermore, HBV DNA revealed a low recurrence rate. The difference between these two groups was not significantly significant. Conclusions: PEG-interferon α-2a and PEG-interferon α-2b are effective antiviral drugs for the treatment of HBeAg-positive CHB, which has a HBsAg seroconversion rate of more than 5%. Furthermore, this sustained response effect was maintained at the 24th and 48th week of follow-up after withdrawal.
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- 2017
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24. Aeroembolism in left atrium during catheter ablation of atrial fibrillation in a patient with dextrocardia: a case report and review of the literature
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Yun-fan Wang, Xue-jiang Cen, Jian-wu Yu, Bai-ming Qu, and Li-hong Wang
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Aeroembolism ,Atrial fibrillation ,Catheter ablation ,Dextrocardia ,Case report ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Air embolus penetrating into heart chamber as a complication during percutaneous radiofrequency catheter ablation has been infrequently reported. Case presentation A 55-year-old man with dextrocardia who suffered from abdominal pain was suspected to have multiple arterial thromboembolisms, which might have originated from left atrium thrombosis since he had atrial fibrillation. He received oral anticoagulant therapy and catheter ablation of the arrhythmia. During the ablation procedure, an iatrogenic aeroembolism penetrated into the left atrium due to improper operation. Ultimately, the entire air embolus was extracted from the patient, who was free of any aeroembolism events thereafter. Conclusions It is essential for an operator to pay full attention to all details of the procedure to avoid an aeroembolism during catheter ablation. In case of aeroembolism, removal by aspiration is an optimal and effective treatment.
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- 2017
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25. Apigenin Inhibits IL-6 Transcription and Suppresses Esophageal Carcinogenesis
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Jian-Ge Qiu, Lin Wang, Wen-Jing Liu, Ju-Feng Wang, Er-Jiang Zhao, Feng-Mei Zhou, Xiang-Bo Ji, Li-Hong Wang, Zhong-Kun Xia, Wei Wang, Marie Chia-mi Lin, Ling-Zhi Liu, Ying-Xue Huang, and Bing-Hua Jiang
- Subjects
apigenin ,esophagus cancer ,interleukin 6 ,tumor growth ,inhibition ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Esophagus cancer is the seventh cause of cancer-related deaths globally. In this study, we analyzed interleukin 6 (IL-6) gene expression in human esophagus cancer patients and showed that IL-6 mRNA levels are significantly higher in tumor tissues and negatively correlated with overall survival, suggesting that IL-6 is a potential therapeutic target for esophagus cancer. We further demonstrated that apigenin, a nature flavone product of green plants, inhibited IL-6 transcription and gene expression in human esophagus cancer Eca-109 and Kyse-30 cells. Apigenin significantly and dose-dependently inhibited cell proliferation and promoted apoptosis while stimulating the cleaved PARP (poly ADP-ribose polymerase) (C-PARP) and caspase-8 expression. It suppressed VEGF (Vascular endothelial growth Factor) expression and tumor-induced angiogenesis. Pretreatment of cells with IL-6 could completely reverse apigenin-induced cellular changes. Finally, using a preclinical nude mice model subcutaneously xenografted with Eca-109 cells, we demonstrated the in vivo antitumor activity and mechanisms of apigenin. Taken together, this study revealed for the first time that apigenin is a new IL-6 transcription inhibitor and that inhibiting IL-6 transcription is one of the mechanisms by which apigenin exhibits its anticancer effects. The potential clinical applications of apigenin in treating esophagus cancer warrant further investigations.
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- 2019
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26. Epidemiological Research on Hand, Foot, and Mouth Disease in Mainland China
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Zhi-Chao Zhuang, Zeng-Qiang Kou, Yong-Juan Bai, Xiang Cong, Li-Hong Wang, Chun Li, Li Zhao, Xue-Jie Yu, Zhi-Yu Wang, and Hong-Ling Wen
- Subjects
HFMD ,enterovirus ,epidemiology ,etiology ,influencing factor ,Microbiology ,QR1-502 - Abstract
Hand, foot, and mouth disease (HFMD), which has led to millions of attacks and several outbreaks across the world and become more predominant in Asia-Pacific Region, especially in Mainland China, is caused by several Human Enteroviruses including new enterovirus, coxsakievirus and echovirus. In recent years, much research has focused on the epidemiological characteristics of HFMD. In this article, multiple characteristics of HFMD such as basic epidemiology, etiology and molecular epidemiology; influencing factors; detection; and surveillance are reviewed, as these can be help protect high risks groups, prevalence prediction and policy making for disease prevention.
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- 2015
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27. CELSR1 Promotes Neuroprotection in Cerebral Ischemic Injury Mainly through the Wnt/PKC Signaling Pathway
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Li-Hong Wang, Geng-Lin Zhang, Xing-Yu Liu, Ai Peng, Hai-Yuan Ren, Shu-Hong Huang, Ting Liu, and Xiao-Jing Wang
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celsr1 ,neurogenesis ,angiogenesis ,stroke ,wnt/pkc pathway ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Cadherin epidermal growth factor (EGF) laminin G (LAG) seven-pass G-type receptor 1 (CELSR1) is a member of a special subgroup of adhesion G protein-coupled receptors. Although Celsr1 has been reported to be a sensitive gene for stroke, the effect of CELSR1 in ischemic stroke is still not known. Here, we investigated the effect of CELSR1 on neuroprotection, neurogenesis and angiogenesis in middle cerebral artery occlusion (MCAO) rats. The mRNA expression of Celsr1 was upregulated in the subventricular zone (SVZ), hippocampus and ischemic penumbra after cerebral ischemic injury. Knocking down the expression of Celsr1 in the SVZ with a lentivirus significantly reduced the proliferation of neuroblasts, the number of CD31-positive cells, motor function and rat survival and increased cell apoptosis and the infarct volume in MCAO rats. In addition, the expression of p-PKC in the SVZ and peri-infarct tissue was downregulated after ischemia/ reperfusion. Meanwhile, in the dentate gyrus of the hippocampus, knocking down the expression of Celsr1 significantly reduced the proliferation of neuroblasts; however, it had no influence on motor function, cell apoptosis or angiogenesis. These data indicate that CELSR1 has a neuroprotective effect on cerebral ischemia injury by reducing cell apoptosis in the peri-infarct cerebral cortex and promoting neurogenesis and angiogenesis, mainly through the Wnt/PKC pathway.
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- 2020
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28. Diagnostic Values of Vascular Endothelial Growth Factor and Epidermal Growth Factor Receptor for Benign and Malignant Hydrothorax
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Yan Gu, Min Zhang, Guo-Hua Li, Jun-Zhen Gao, Liping Guo, Xiao-Juan Qiao, Li-Hong Wang, Lan He, Mei-Ling Wang, Li Yan, and Xiu-Hua Fu
- Subjects
Enzyme-linked Immunosorbent Assay ,Epidermal Growth Factor Receptor ,Fluorescence In Situ Hybridization ,Hydrothorax ,Vascular Endothelial Growth Factor ,Medicine - Abstract
Background: Hydrothorax, as one of the common complications of malignant tumors, still cannot be sensitively detected in clinical practice, thus requiring a sensitive, specific method for diagnosis. The aim of this study was to analyze the correlation between levels of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in patients with benign and malignant hydrothorax. Methods: The contents of VEGF in the pleural effusion and serum of the patients with malignant pleural effusion (n = 35) and benign pleural effusion (n = 30) were detected by double antibody sandwich enzyme linked immunosorbent assay. The gene copy number level of EGFR in pleural effusion was detected by fluorescence in situ hybridization (FISH). The points with the highest sensitivity and specificity were selected as the critical values to calculate the diagnostic value of the VEGF in pleural effusion and serum, and EGFR gene copy number in pleural effusion. Results: The contents of VEGF in pleural effusion and serum of patients with malignant hydrothorax were (384.91 ± 120.18), and (129.62 ± 46.35) ng/L, respectively, which were significantly higher than those of the patients with benign hydrothorax (207.97 ± 64.04), (63.49 ± 24.58) ng/L (P < 0.01). The sensitivity and specificity of detecting VEGF in pleural effusion were 80.0% and 96.7% (the boundary value was 297.06 ng/L), respectively for diagnosing benign and malignant hydrothorax. The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax. The diagnostic efficiencies of EGFR and VEGF in hydrothorax were similar. There was a significant correlation between EGFR and VEGF in hydrothorax (P < 0.01). Conclusions: VEGF and EGFR play important roles in the formation of pleural effusion. VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions. It is feasible to detect the gene copy number of the pleural effusion cell mass EGFR by FISH technique. Joint detection can improve the diagnostic sensitivity.
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- 2015
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29. Determinants of Quality of Life for Breast Cancer Patients in Shanghai, China.
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Bei Yan, Li-Ming Yang, Li-Peng Hao, Chen Yang, Lei Quan, Li-Hong Wang, Zheng Wu, Xiao-Pan Li, Yu-Tang Gao, Qiao Sun, and Jian-Min Yuan
- Subjects
Medicine ,Science - Abstract
PURPOSE:To evaluate the association of social support status, health insurance and clinical factors with the quality of life of Chinese women with breast cancer. METHODS:Information on demographics, clinical characteristics, and social support status was collected from 1,160 women with newly diagnosed breast cancer in Shanghai, China. The Perceived Social Support Scale was used to assess different sources of social support for breast cancer patients. The quality of life was evaluated using the Functional Assessment of Cancer Therapy-Breast Cancer that consisted of five domains: breast cancer-specific, emotional, functional, physical, and social & family well-being. Multivariate linear regression models were used to evaluate the associations of demographic variables, clinical characteristics, and social support status with the quality of life measures. RESULTS:Adequate social support from family members, friends and neighbors, and higher scores of Perceived Social Support Scale were associated with significantly improved quality of life of breast cancer patients. Higher household income, medical insurance plans with low copayment, and treatment with traditional Chinese medicine for breast cancer all were associated with higher (better) scores of quality of life measures whereas patients receiving chemotherapy had significantly lower scores of quality of life. CONCLUSION:Social support and financial aids may significantly improve the quality of life of breast cancer survivors.
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- 2016
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30. Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
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Geng-Lin Zhang, Li-Hong Wang, Xing-Yu Liu, Ya-Xuan Zhang, Meng-Yang Hu, Lin Liu, Yuan-Yuan Fang, Yu Mu, Yan Zhao, Shu-Hong Huang, Ting Liu, and Xiao-Jing Wang
- Subjects
CDNF ,stroke ,oxygen–glucose depletion ,neuroprotection ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic neurons. The function of CDNF during brain ischemia is still not known. MANF is identified as an endoplasmic reticulum (ER) stress protein; however, the role of CDNF in ER stress remains to be fully elucidated. Here, we test the neuroprotective effect of CDNF on middle cerebral artery occlusion (MCAO) rats and neurons and astrocytes treated with oxygen–glucose depletion (OGD). We also investigate the expression of CDNF in cerebral ischemia and in primary neurons treated with ER stress-inducing agents. Our results show that CDNF can significantly reduce infarct volume, reduce apoptotic cells and improve motor function in MCAO rats, while CDNF can increase the cell viability of neurons and astrocytes treated by OGD. The expression of CDNF was upregulated in the peri-infarct tissue at 2 h of ischemia/24 h reperfusion. ER stress inducer can induce CDNF expression in primary cultured neurons. Our data indicate that CDNF has neuroprotective effects on cerebral ischemia and the OGD cell model and the protective mechanism of CDNF may occur through ER stress pathways.
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- 2018
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31. Attribute Reduction and Information Granularity
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Li-hong Wang and Geng-feng Wu
- Subjects
Rough Set ,Reducts Combination ,Attribute Reduct ,Information Granularity ,Information technology ,T58.5-58.64 ,Communication. Mass media ,P87-96 - Abstract
In the view of granularity, this paper analyzes the influence of three attribute reducts on an information system, finding that the possible reduct and m - decision reduct will make the granule view coarser, while discernible reduct will not change the granule view. In addition, we investigate the combination of reducts from two partial information systems in parallel or in incremental data mining and urge that the union of partial possible reducts can be regarded as a possible reduct for union of partial information systems.
- Published
- 2003
32. Adiponectin Induces Oncostatin M Expression in Osteoblasts through the PI3K/Akt Signaling Pathway
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Chen-Ming Su, Wei-Lin Lee, Chin-Jung Hsu, Ting-Ting Lu, Li-Hong Wang, Guo-Hong Xu, and Chih-Hsin Tang
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rheumatoid arthritis ,adiponectin ,oncostatin M ,osteoblasts ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Rheumatoid arthritis (RA), a common autoimmune disorder, is associated with a chronic inflammatory response and unbalanced bone metabolism within the articular microenvironment. Adiponectin, an adipokine secreted by adipocytes, is involved in multiple functions, including lipid metabolism and pro-inflammatory activity. However, the mechanism of adiponectin performance within arthritic inflammation remains unclear. In this study, we observed the effect of adiponectin on the expression of oncostatin M (OSM), a pro-inflammatory cytokine, in human osteoblastic cells. Pretreatment of cells with inhibitors of phosphatidylinositol 3-kinase (PI3K), Akt, and nuclear factor (NF)-κB reduced the adiponectin-induced OSM expression in osteoblasts. Stimulation of the cells with adiponectin increased phosphorylation of PI3K, Akt, and p65. Adiponectin treatment of osteoblasts increased OSM-luciferase activity and p65 binding to NF-κB on the OSM promoter. Our results indicate that adiponectin increased OSM expression via the PI3K, Akt, and NF-κB signaling pathways in osteoblastic cells, suggesting that adiponectin is a novel target for arthritis treatment.
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- 2015
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33. 12-(4-Chlorophenyl)-9,9-dimethyl-9,10-dihydro-8H-benzo[a]xanthen-11(12H)-one
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Li-Hong Wang and De-Ling Li
- Subjects
Crystallography ,QD901-999 - Abstract
The title compound, C25H21ClO2, was synthesized via the three-component coupling of 4-chlorobenzaldehyde, 2-naphthol and 5,5-dimethylcyclohexane-1,3-dione. The pyran ring adopts a boat conformation, while the cyclohexenone ring is in an envelope conformation. The 4-chlorophenyl ring is almost perpendicular to the pyran ring [dihedral angle = 87.39 (1)°]. In the crystal, molecules are connected by intermolecular C—H...O hydrogen bonds.
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- 2010
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34. 12-(2-Methoxyphenyl)-9,9-dimethyl-8,9-dihydro-12H-benzo[a]xanthen-11(10H)-one
- Author
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De-Ling Li and Li-Hong Wang
- Subjects
Crystallography ,QD901-999 - Abstract
The title compound, C26H24O3, was synthesized via the coupling of 2-methoxybenzaldehyde, 2-naphthol and 5,5-dimethylcyclohexane-1,3-dione. The pyran ring adopts a boat conformation, while the cyclohexenone ring is in an envelope conformation. The 2-methoxyphenyl ring is almost perpendicular to the plane through the four C atoms of the pyran ring [dihedral angle = 88.76 (9)°].
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- 2010
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35. 9,9-Dimethyl-12-(4-nitrophenyl)-9,10-dihydro-12H-benzo[a]xanthen-11(8H)-one
- Author
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Li-Hong Wang and De-Ling Li
- Subjects
Crystallography ,QD901-999 - Abstract
In the molecular structure of the title compound, C25H21NO4, the pyran ring adopts a flattened boat conformation, while the cyclohexenone ring is in an envelope conformation. The 4-nitrophenyl ring is almost perpendicular to the pyran ring [dihedral angle = 89.39 (1)°]. In the crystal, molecules are connected by intermolecular C—H...O hydrogen bonds.
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- 2009
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36. A Multi-Graph Laplacian Regularized Low-Rank Representation method for cancer sample clustering with integrated TCGA data.
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Juan Wang 0003, Li-Hong Wang, Tian-Jing Qiao, and Shasha Yuan
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- 2022
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37. Multi-View Random-Walk Graph Regularization Low-Rank Representation for Cancer Clustering and Differentially Expressed Gene Selection.
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Juan Wang 0003, Li-Hong Wang, Jin-Xing Liu 0001, Xiang-Zhen Kong, and Shengjun Li
- Published
- 2022
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38. Iridium-Catalyzed Asymmetric Hydrogenation of Heteroaromatics with Multiple N Atoms via Substrate Activation: An Entry to 4,5,6,7-Tetrahydropyrazolo[1,5‑a]pyrimidine-3-carbonitrile Core of a Potent BTK Inhibitor.
- Author
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Chen, Mu-Wang, Li, Hong-Wang, Wang, Ying-Qi, Wu, Bo, Liu, Zheng, Lai, Xinzhong, Deerberg, Joerg, and Zhou, Yong-Gui
- Published
- 2024
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39. Attractors and asymptotic regularity for nonclassical diffusion equations in locally uniform spaces with critical exponent.
- Author
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Fanghong Zhang, Li-hong Wang, and Jin-ling Gao
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- 2016
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40. Hole Transfer Prompted by Viscous Oligomer Solid Additives in Non-Fullerene Bulk-Heterojunction Layers
- Author
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Yan Chen, Lin Hu, Zhen Su, Xiao-Feng Zhang, Hui Liu, Li-Hong Wang, Bin Huang, Zaifang Li, and Shi-Yong Liu
- Subjects
Polymers and Plastics ,Process Chemistry and Technology ,Organic Chemistry - Published
- 2022
41. Pot- and atom-economic synthesis of oligomeric non-fullerene acceptors via C–H direct arylation
- Author
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Li-Hong Wang, Xian-Jie Chen, Dong-Nai Ye, Hui Liu, Yan Chen, Ai-Guo Zhong, Chang-Zhi Li, and Shi-Yong Liu
- Subjects
Polymers and Plastics ,Organic Chemistry ,Bioengineering ,Biochemistry - Abstract
Three long-chain oligomeric acceptors with a stepwise increase in conjugation length are obtained via three successive one-pot reactions and a systematic structure–property–performance relationship study was carried out.
- Published
- 2022
42. Isolated metastasis of mediastinal carcinoid to the left ventricle: A case report
- Author
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Hong-Yan Li, Hui-Hui Wu, and Li-Hong Wang
- Subjects
Pathology ,medicine.medical_specialty ,Gastrointestinal tract ,business.industry ,Carcinoid tumors ,Mediastinum ,Neuroendocrine tumors ,medicine.disease ,digestive system diseases ,Metastasis ,medicine.anatomical_structure ,Peritoneum ,Ventricle ,Medicine ,Radiology, Nuclear Medicine and imaging ,Serotonin ,Cardiology and Cardiovascular Medicine ,business ,neoplasms - Abstract
Carcinoid tumors are uncommon neuroendocrine tumors that produce high levels of serotonin and are mainly located in the gastrointestinal tract and bronchopulmonary system but very rarely in the mediastinum. These are slow-growing tumors that most commonly present metastatic lesions in the liver, followed by the bones, lungs, and peritoneum. Solid cardiac metastases from carcinoid tumors have seldom been reported. In this case report, we present a patient with a rare localized metastasis in the left ventricle with an antecedent history of resected carcinoid tumor of the anterior mediastinum.
- Published
- 2021
43. MiRNA-30e downregulation increases cancer cell proliferation, invasion and tumor growth through targeting RPS6KB1
- Author
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Lin Wang, Jian-Ge Qiu, Bing-Hua Jiang, Xiang-Bo Ji, Ling-Zhi Liu, Yun-Xia Xie, Zhong-Kun Xia, Li-Hong Wang, and Wen-Jing Liu
- Subjects
Aging ,Esophageal Neoplasms ,clinical outcome ,Down-Regulation ,Biology ,esophageal carcinoma ,Esophagus ,Downregulation and upregulation ,Cell Line, Tumor ,microRNA ,medicine ,Humans ,Neoplasm Invasiveness ,Survival rate ,Cell Proliferation ,Tube formation ,Cell growth ,Cancer ,Ribosomal Protein S6 Kinases, 70-kDa ,Cell Biology ,medicine.disease ,Blot ,RPS6KB1 ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,tumor growth ,Cancer cell ,miR-30e ,Cancer research ,Research Paper - Abstract
Human esophagus carcinoma (EC) is one of the most common malignant tumors, especially in Africa and Asia including China. In EC initiation and progression, genetic and epigenetic aberrations have been reported to play a major role, but the underlying molecular mechanisms are largely unknown. In this study, the miR-30e levels were analyzed in human EC tissues and TCGA databases, and the results demonstrated that miR-30e expression in EC tissues was significantly decreased compared to adjacent normal tissues. To further investigate the role of miR-30e in cancer cells, we found that forced expression of miR-30e dramatically inhibited cell proliferation, invasion, tube formation, and colony formation of cancer cells. To determine the underlying mechanism of miR-30e, we found that RPS6KB1 was a direct target of miR-30e by binding to its 3'-UTR, which was verified by luciferase activity assay using reporters with wild-type miR-30e and its seed sequence mutant constructs and Western blotting assay. In vivo experiment showed that miR-30e overexpression significantly inhibited tumor growth and decreased RPS6KB1 expression in xenografts. In EC, high expression of RPS6KB1 in tumor tissues indicated poor prognosis of patients with less survival rate. High levels of RPS6KB1 and low levels of miR-30e closely correlated poor survival of patients with several other types of cancer. These findings show that miR-30e and its target RPS6KB1 are important in cancer development and clinical outcomes, and miR-30e/RPS6KB1 is a potential future therapeutic pathway for EC intervention.
- Published
- 2021
44. Levosimendan administration is not associated with increased risk of bleeding and blood transfusion requirement in patients undergoing off-pump coronary artery bypass grafting: a retrospective study from single center
- Author
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Li-Xian He, Xiao-Hong Wang, Li-Hong Wang, Run-Qiao Fu, Jie-chao Tan, Yun-Tai Yao, and Yong Lin
- Subjects
Advanced and Specialized Nursing ,Blood transfusion ,business.industry ,medicine.medical_treatment ,Mortality rate ,Retrospective cohort study ,General Medicine ,Levosimendan ,Single Center ,medicine.anatomical_structure ,Anesthesia ,medicine ,Radiology, Nuclear Medicine and imaging ,Fresh frozen plasma ,Cardiology and Cardiovascular Medicine ,business ,Safety Research ,medicine.drug ,Off-pump coronary artery bypass ,Artery - Abstract
Background: Levosimendan (LEVO) is a positive inotropic drug which could increase myocardial contractility and reduce the mortality rate in cardiac surgical patients. However, Whether LEVO is associated with postoperative bleeding and blood transfusion in cardiac surgical patients is controversial. Therefore, the current study was designed to investigate the impact of LEVO administration on bleeding and blood transfusion requirement in off-pump coronary artery bypass grafting (OPCAB) patients. Methods: In a retrospective analysis, a total of 292 patients, aged 40–87 years, undergoing elective OPCAB between January 2019 and July 2019, were divided into LEVO group ( n = 151) and Control group ( n = 141). Patients in LEVO group continuously received LEVO at a rate of 0.1–0.2 μg kg−1 min−1 after anesthesia induction until 24 hours after OPCAB or patients in Control group received no LEVO. The primary outcome was postoperative chest drainage volume. The secondary outcomes were reoperation for postoperative bleeding, transfusion requirement of red blood cells (RBCs), fresh frozen plasma (FFP) and platelet concentrate (PC), etc. Comparisons of two groups were performed with the Student’s t-test or Wilcoxon-Mann-Whitney test. Results: There was no significant difference with respect to chest drainage volume ((956.29 ± 555.45) ml vs (1003.19 ± 572.25) ml, p = 0.478) and the incidence of reoperation for postoperative bleeding (1.32% vs 1.42%, p = 0.945) between LEVO group and Control group. The transfusion incidence and volume of allogeneic RBCs, FFP, and PC were comparable between two groups. Conclusions: LEVO administration was neither associated with more postoperative blood loss nor increased allogeneic blood transfusion requirement in OPCAB patients.
- Published
- 2021
45. A Multi-Graph Laplacian Regularized Low-Rank Representation method for cancer sample clustering with integrated TCGA data
- Author
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Juan Wang, Li-Hong Wang, Tian-Jing Qiao, and Sha-Sha Yuan
- Published
- 2022
46. Transformation of hydroxylamine to nitrosated and nitrated products during advanced oxidation process
- Author
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Zi-jun Dong, Cheng-chun Jiang, Yue Zhou, Jie-bin Duan, Li-hong Wang, Su-yan Pang, Jin Jiang, and Xiao-hui Sun
- Subjects
Environmental Engineering ,Health, Toxicology and Mutagenesis ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Abstract
Recently, hydroxylamine (HAm) was introduced to drive advanced oxidation processes (AOPs) for removing organic contaminants. However, we found that HAm-driven Cu(II)/peroxymonosulfate oxidation of phenol produced p-nitrosophenol, 2-nitrophenol and 4-nitrophenol. The total nitro(so) products accounted for approximately 25.0 % of the phenol transformation at certain condition. SO
- Published
- 2022
47. Risk Factors and Outcomes for Preoperative Asymptomatic Pulmonary Embolism in Patients Aged 60 Years and Over with Hip Fracture
- Author
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Guo-Jian Shentu, Guo-Hong Xu, Li-Hong Wang, Fang-Lun Zhou, and Chengqian Dai
- Subjects
Male ,medicine.medical_specialty ,Asymptomatic ,Time-to-Treatment ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Humans ,Medicine ,Orthopedics and Sports Medicine ,Prospective Studies ,Prospective cohort study ,Aged ,Preoperative ,Aged, 80 and over ,Orthopedic surgery ,030222 orthopedics ,Univariate analysis ,Hip fracture ,Clinical Article ,business.industry ,Incidence ,Incidence (epidemiology) ,Pulmonary embolism ,Odds ratio ,Middle Aged ,medicine.disease ,Exact test ,Asymptomatic Diseases ,Preoperative Period ,Hip fractures ,Clinical Articles ,Female ,Surgery ,medicine.symptom ,business ,Biomarkers ,030217 neurology & neurosurgery ,RD701-811 - Abstract
Objective To investigate the risk factors for, and outcomes of, preoperative asymptomatic pulmonary embolism (PE) in patients ≥60 years old following delayed operation for hip fracture. Methods From March 2017 to December 2018, 90 patients aged ≥60 years with hip fracture who suffered a delay in surgery were recruited to this prospective study following admission to our hospital. Computed tomography pulmonary angiography (CTPA) was used to detect preoperative asymptomatic PE and calculated its incidence. Time from injury to admission, baseline characteristics, medical comorbidities, and blood biomarker levels were evaluated as potential risk factors. Logistic regression analysis was used to identify risk factors. Mortality and major bleeding events were recorded and compared between individuals with PE and without. Data were analyzed by t‐test, Mann–Whitney U test, χ 2 test, Fisher's exact test, and logistic regression analysis. Results The incidence of preoperative asymptomatic PE was 18.9% (17/90 patients). In the univariate analysis, the risk factors for preoperative asymptomatic PE were male sex, hypertension, cerebrovascular accident, smoking, plasma D‐dimer level, potassium level, urea level, creatinine level, and cysteine level. Multivariate logistic regression analysis showed that the risk of preoperative asymptomatic PE was higher in patients with hypertension (odds ratio [OR] = 10.048; 95% confidence interval [CI], 1.118–90.333), cerebrovascular accident (OR = 20.135; 95% CI, 1.875–216.164), smoking (OR = 48.741; 95% CI, 4.155–571.788), high plasma D‐dimer levels (OR = 1.200; 95% CI, 1.062–157.300), and high plasma potassium levels (OR = 12.928; 95% CI, 1.062–157.300). All patients were followed up for 21.0 months (range, 2 to 36 months). Mortality within the first year postoperatively was higher in patients with PE (29.41% vs 9.59%, P = 0.046). Conclusions In view of the high incidence of preoperative asymptomatic PE and the inferior prognosis in individuals with PE, routine CTPA examination for preoperative asymptomatic PE could be useful for patients aged ≥60 years with hip fracture for whom surgery is delayed., The overall incidence of preoperative asymptomatic PE was 18.9% in patients ≥60 years old following delayed operation for hip fracture. Individuals with preoperative asymptomatic PE have an inferior prognosis. Hypertension, cerebrovascular accident, smoking, high plasma D‐dimer levels, and high plasma potassium levels were associated with preoperative asymptomatic PE in these patients.
- Published
- 2021
48. Investigation and evaluation of geological hazards using remote sensing in the Tibetan part of national highway 214.
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An-xin Lu, Li-hong Wang, Zhi-yu Jia, Lin-qing Yu, and De-fu Ran
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- 2007
- Full Text
- View/download PDF
49. Using remote sensing in the Tibetan part of national highway 317.
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Lin-qing Yu, An-xin Lu, Li-hong Wang, Zhi-yu Jia, and De-fu Ran
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- 2007
- Full Text
- View/download PDF
50. The study of disaster investigation by using remote sensing on the sichuan-tibet highway in tibet.
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Li-hong Wang, An-xin Lu, Zhi-yu Jia, and Lin-qing Yu
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- 2007
- Full Text
- View/download PDF
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