Search

Lipid droplets (LDs) and lysosomes are vital organelles that play crucial roles in various physiological and pathological processes. However, simultaneous two-color visualization of these two organelles using a single probe for cell homeostasis monitoring remains a challenge due to the lack of rational design strategies. To address this issue, we have developed an aggregation-induced emission (AIE) fluorescent probe named TPE-NDI-Mor with an electron donor (D)–acceptor (A) structure, which can stain both LDs and lysosomes with high selectivity through green and red fluorescence imaging, respectively. A detailed mechanism study revealed that TPE-NDI-Mor, with a twisted intramolecular charge transfer (TICT) effect, shows a high affinity for a polar microenvironment. Additionally, the probe also demonstrates good stability, high anti-interference performance and a large Stokes shift, making it suitable for visualizing cell homeostasis and further disease diagnosis by tracking the dynamic changes of LDs and lysosomes. [ABSTRACT FROM AUTHOR]

In order to study the dyeing performance of Peristrophe roxburghiana on silk,Peristrophe roxburghiana dye extracted from water bath was used to dye mulberry silk fabrics. The pretreatment and dyeing process of mulberry silk dyed with Peristrophe roxburghiana dye was described, and the effects of dyeing temperature, dyeing time and bath pH value on the dyeing effect of mulberry silk fabrics was analyzed. By comparing the K/S values, the best dyeing conditions were found, and the color fastness to washing and sun exposure of mulberry silk dyed with Peristrophe roxburghiana dye was investigated. The experimental results show that the best dyeing process for mulberry silk was as follows: alum pre-mordant dyeing process, alum 5% (owf), bath ratio 1 :100, pH value of dye solution 10. 0, dyeing temperature 30 °C, dyeing time 30 min. [ABSTRACT FROM AUTHOR]

Aluminum (Al 3+ ) and fluorine (F - ) ions can be easily enriched in tea plants. When they excessively accumulate in tea, they can affect the health of tea lovers. Herein, a simple, highly sensitive and selective fluorescent probe (named BHMP) for Al 3+ and F - detection was developed through a one-step condensation reaction, in which benzothiazole acted as a fluorophore and acceptor and hydrazine-Schiff base as a recognition unit. The probe was characterized comprehensively using spectroscopic methods, and the structure-activity relationship was systematically researched through crystal structure and theoretical calculations. Its sensitivity was measured via the fluorescent titration experiment, and the limit of detection (LOD) towards Al 3+ was up to 1.04 × 10 -8 mol L -1 . Furthermore, we successfully utilized BHMP to visually detect the presence of Al 3+ in living cells and tea tree roots through fluorescence confocal imaging. The successful detection of Al 3+ in tea tree roots indicated that BHMP could be used as a candidate fluorescent chemosensor to dynamically monitor the variation in enriched Al 3+ under the influence of the environment during tea tree growth. Our study provides a reference for the control of Al 3+ concentration during the growth of tea plants and provides new insights into improving tea quality control.