Search

Social adversity not only causes severe psychological diseases but also may improve people’s ability to learn and grow. However, the beneficial effects of social adversity are often ignored. In this study, we investigated whether and how social adversity affects learning and memory in a mouse social defeat stress (SDS) model. A total of 652 mice were placed in experimental groups of six to 23 mice each. SDS enhanced spatial, novelty, and fear memory with increased synaptosome associated protein 25 ( SNAP-25) level and dendritic spine density in hippocampal neurons among young but not middle-aged mice. Chemogenetic inhibition of hippocampal CaMK2A + neurons blocked SDS-induced enhancement of learning or memory. Knockdown of SNAP-25 or blockade of N-methyl-D-aspartate (NMDA) receptor subunit GluN2B in the hippocampus prevented SDS-induced learning memory enhancement in an emotion-independent manner. These findings suggest that social adversity promotes learning and memory ability in youths and provide a neurobiological foundation for biopsychological antifragility.

To explore the clinical efficacy of focused extracorporeal shock wave therapy with centrifugal exercise in the treatment of greater trochanteric pain syndrome.From September 2017 to June 2019, 53 eligible cases of greater trochanteric pain syndrome were randomly divided into observation group (29 cases) and control group (24 cases). In observation group, there were 8 males and 21 females, aged from 38 to 62 years old with an average of (49.96±6.39) years old; the course of disease ranged from 6 to 13 months with an average of (8.58±1.99) months;treated with focused extracorporeal shock wave therapy with centrifugal exercise. In control group, there were 5 males and 19 females, aged from 39 to 62 years old with an average of (52.79±5.86) years old;the course of disease ranged from 6 to 14 months with an average of (9.04±2.51) months;treated with centrifugal exercise alone. Visual analogue scale (VAS) and hip Harris score were measured before ESWT treatment and at 1, 2, and 6 months to evaluate relieve degree of pain and functional recovery of hip joint, respectively.At 1 month after treatment, there were no significant differences in VAS, hip Harris score and treatment success rate (allIn treatment of greater trochanteric pain syndrome, focused extracorporeal shock wave therapy with centrifugal exercise could significantly relieve symptoms of lateral hip pain, improve functional recovery of hip joint with good safety. This treatment strategy is worthy of application and promotion in clinical practice.

To investigate the influence of MRD status in newly diagnosed MM patients with VGPR and above after treatment on clinical prognosis.Clinical data of 210 newly diagnosed MM patients with VGPR and above after treatment in Fifth People's Hospital of Chendu city. from January 2010 to January 2018 were collected and retrospectively analyzed. The patients were divided into 2 groups: group A (152 patients with MRDThere were no significant difference in clinical characteristics between the patients in 2 groups (P＞0.05). Single factor analysis showed that ASCT and MRD status were related with progression free survival of patients with newly diagnosed MM (P＜0.05). Multivariate analysis by Cox regression model showed that MRDMRD治疗后达VGPR及以上的初治MM患者MRD状态对临床预后的影响.探讨治疗后达非常好的部分缓解（VGPR）及以上初治多发性骨髓瘤（MM）患者微小残留病变（MRD）状态对临床预后的影响。方法：。结果：。结论：。.回顾性分析2010年1月-2018年1月于成都市第五人民医院治疗后达VGPR及以上的初治MM患者共210例临床资料, 其中MRD2组患者临床特征资料比较无显著性差异（P＞0.05）。单因素分析结果显示, ASCT和MRD状态与初治MM患者无进展生存时间有关（P＜0.05）；Cox回归模型多因素分析结果显示, MRD持续阳性是初治MM患者无进展生存时间的独立危险因素（P＜0.05）；A组患者随访2年累积无进展生存率显著高于B组患者（P＜0.05）。持续MRD治疗后达VGPR及以上的初治MM患者治疗后持续MRD

To investigate the mechanisms through which myocyte large-conductance CaMiddle cerebral arteries (MCA) were obtained from 8-week-old male Wistar rats after anaesthetized. Middle cerebral arterial smooth muscle cells were enzymatically isolated. Whole cell recording mode of patch clamp technique was used to measure the current density of BK① Melatonin markedly increased the whole-cell BKMelatonin mediates vasodilation of MCA through the activation of BK

Objective To investigate the effect of exercise during pregnancy on L-type Ca2+ (CaV1.2) channel in mesenteric artery from spontaneously hypertensive rats (SHR) offspring. Methods Female (11 weeks old) and male (12 weeks old) SHR, female (11 weeks old) and male (12 weeks old) WKY rats were selected to use for breeding. The day when the vaginal bolt was found was considered day 1 of gestation. The pregnant rats were randomly divided into four groups: WKY sedentary group (WKY-SED), WKY exercise group (WKY-EX), SHR sedentary group (SHR-SED) and SHR exercise group (SHR-EX). The exercise groups were subjected to swimming at the first day of pregnancy, 1h/d，6 days/week for 3 weeks. The 6-month-old male offspring were operated with femoral arterial and venous cannulation, and the blood pressure after intravenous (i.v.) injection of CaV1.2 channel opener BayK8644 and blocker nifedipine were monitored in vivo. In vitro study, the mesenteric arteries were removed and used for isometric contraction studies. The non-selective NOS inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μM) was added after 60mM KCl measurement. To investigate the contribution of CaV1.2 channels in vascular tone regulation, the vascular responses to nifedipine (10−9–10−5M) were examined. Western blot was applied to examine the protein expression levels of CaV1.2 channel. Results (1) The mean arterial pressure(MAP) were higher in the 6M offspring of SHR-SED group than that of WKY-SED group (P＜0.01), but there was no significant difference between the 6M offspring of SED and Ex groups. (2) The pressor responses induced by i.v. injection of BayK8644（0.1mg/Kg）were increased in the 6M offspring of SHR-SED group (P＜0.05) compared with the WKY-SED group. Exercise during pregnancy markedly decreased the pressor responses in 6M offspring of SHR-EX group (P＜0.05). (3) Compared with the 6M offspring of WKY-SED group, the depressor responses induced by i.v. injection of nifedipine（1mg/Kg）were increased in the 6M offspring of SHR-SED group (P＜0.01). Exercise during pregnancy markedly attenuated the depressor responses in 6M offspring of SHR-EX group (P＜0.05). (4) The isometric contraction study revealed that nifedipine induced concentration-dependent vasorelaxation in mesenteric artery precontracted with noradrenaline. The sensitivity of tissues to nifedipine in 6M offspring of SHR-SED group was significantly higher than that of WKY-SED group (P＜0.01). Exercise during pregnancy normalized the increased sensitivity of tissues to nifedipine in 6M offspring of SHR (P＜0.05). (5) Compared with the 6M offspring of WKY-SED group, the protein expression of CaV1.2α1C was significantly increased in SHR-SED group(P＜0.01). Exercise during pregnancy markedly inhibited the expression of CaV1.2α1C in 6M offspring of SHR-EX group (P＜0.05). Conclusions Pregnancy exercise has no significant effect on basic blood pressure in 6M offspring of SHR; but the increased function and protein expression of CaV1.2 channel in 6M offspring of SHR may be normalized by exercise during pregnancy.

For the students of pre-school education, the application ability of modern educational technology is one of the essential qualities, and it is also one of the main qualities of professional development of future kindergarten teachers. We propose to strengthen the students' modern education technology awareness and emotion, strengthen the modern educational technology and teaching design, multimedia computer application and courseware production curriculum, define the modern educational technology learning objectives and task level to establish multiple evaluation and assessment system Measures to strengthen the students' modern education technology to master and apply. Modern education technology is mainly based on modern education theory, the use of information technology means, teaching and learning resources and process analysis and research, to take a systematic approach to teaching, to achieve the desired effect of education. With the continuous development of the era of knowledge economy, computer network technology has been rapid development, in the cause of education has been widely used. In the actual teaching process, the teacher of modern education technology to master the degree and ability to use, will have a direct impact on the teaching effect. And teachers want to master and use the appropriate modern education technology, we must strengthen the study of modern educational technology. This article mainly analyzes the necessity of the students to master the modern educational technology, expounds the present situation of the students' modern educational technology, and puts forward the effective enhancement measures according to the actual situation.

Traditional cam profile curve is not changeable after it is made. So it can’t meet the requirement when the follower needs to change the motion within a short time. Therefore, we designed a new cylindrical cam structure whose profile curve could be reconstructed by adjust inserted depth of the chips. We built its three-dimension object model by SOLIDWORKS and explained its advantages and shortcomings. We illustrated its advantages by provided an example of application. And results show the new cam structure is high versatility and convenient to realize the needs of different follower motion by reconstruct its profile.

Dry anaerobic co-digestion of animal manure and straw was conducted to produce biogas. Startup characteristics and biogas production perform of dry digestion were studied, and the effect of operation temperature and incubation amount on dry digestion was also investigated. The study result showed that under the conditions of C/N=25-30, TS=20% and T=(36±1)°C,dry digestion can start up quickly with acclimated thickening sludge as incubation sludge. Compared to dry digestion of chicken manure and pig manure, dry digestion of cow manure proceeded steadily with high biogas yield. It is found that incubation is necessary in the process of dry digestion and biogas yield increases with more incubation amount. The appreciate incubation ratio is about 10%. Temperature change has apparent effect on biogas production, and it is suggested that mesophilic temperature should be chosen in the scaled project of dry digestion.

The rigid-flexible hybrid modeling of the wind tunnel test platform is established by means of ANSYS and ADAMS for more accurate analysis of the motion performance of the test platform in this paper. We get the kinematical characteristic curve based on the three working conditions of the pitching motion, yawing motion, rolling motion of the test platform. According to the characteristic curve indirectly reflects the rationality of the design configuration. So as to provide fundamental basis for the precision dynamics simulation analysis and make the real physical prototype are offered.

To mimic human mood disorders, traditional chronic stresses and social defeat stress have been developed and widely applied. However, these active stresses do not mimic the emotional flaws induced by stresses, and their input levels vary greatly. Also, emotional stresses resulting from social unobtainability remain largely elusive due to the lack of useful animal models. In this study, we developed a mouse model named “opposite sex contact and isolation” (OSCI) and found that OSCI induced significant social avoidance, anhedonia, and anxiety. These behavioral defects developed differently after 7 days of OSCI. The social avoidance behavior was self-curable while anxiety gradually worsened but was alleviated by re-pairing with the same female partner. Corresponding to the behavior changes, the plasma corticosterone and phosphorylated cAMP response element binding protein levels were decreased in the nucleus accumbens of the mice that experienced isolation. Together, this study has developed a novel strategy for depression/anxiety modeling and shows that OSCI may be a useful tool for studying the lovelorn/lovesick type of depression.

Background Social defeat (SD) stress induces social avoidance and anxiety-like phenotypes. Amygdala is recognized as an emotion-related brain region such as fear, aversion and anxiety. It is conceivable to hypothesize that activation of amygdala is involved in SD-dependent behavioral defects. Results SD model was established using C57BL/6J mice that were physically defeated by different CD-1 mice for 10 days. Stressed mice exhibited decreased social interaction level in social interaction test and significant anxiety-like behaviors in elevated plus maze and open field tests. Meanwhile, a higher phosphorylation of PKA and CREB with a mutually linear correlation, and increased Fos labeled cells in the basolateral amygdala (BLA) were observed. Activation of PKA in the BLA by 8-Br-cAMP, a PKA activitor, significantly upregulated pCREB and Fos expression. To address the role of PKA activation on SD stress-induced social avoidance and anxiety-like behaviors, 8-Br-cAMP or H-89, a PKA inhibitor, was continuously administered into the bilateral BLA by a micro-osmotic pump system during the 10-day SD period. Neither H-89 nor 8-Br-cAMP affected the social behavior. Differently, 8-Br-cAMP significantly relieved anxiety-like behaviors in both general and moderate SD protocols. H-89 per se did not have anxiogenic effect in naïve mice, but aggravated moderate SD stress-induced anxiety-like behaviors. The antidepressant clomipramine reduced SD-induced anxiety and up-regulated pPKA level in the BLA. Conclusions These results suggest that SD-driven PKA activation in the basolateral amygdala is actually a compensatory rather than pathogenic response in the homeostasis, and modulating amygdaloid PKA may exhibit potency in the therapy of social derived disorders. Electronic supplementary material The online version of this article (doi:10.1186/s13041-015-0181-3) contains supplementary material, which is available to authorized users.

Dry anaerobic fermentation of animal manure and straw was conducted to produce biogas. Under C/N=25-30 and TS=20%, three different temperature conditions of room temperature condition(16.5°C-25°C), mesophilic condition(36°C) and thermophilic condition(55°C) were tested to investigate the effect of operation temperature on dry fermentation. The results show that dry fermentation under room temperature starts up slowly with low biogas production, so room temperature is unfit for dry fermentation. Dry fermentation under thermophilic condition and mesophilic condition proceed steadily with high biogas production and mesophilic condition is more suitable for dry fermentation. The organic loading rates under thermophilic condition and mesophilic condition are 1.69 kg•m-3•d-1 and 1.89 kg•m-3•d-1 respectively with total solid (TS) biogas yields of 0.237 m3•kg-1 and 0.208 m3•kg-1, and volumetric biogas yields of 0.401 m3•m-3•d-1 and 0.393 m3•m-3•d-1 respectively. It could provide instructive meaning to the engineering application of dry anaerobic fermentation.

This paper presents a new method for classification of remote sensing image based on multiple classifiers combination. In this method, three supervised classifications such as Mahalanobis Distance, Maximum Likelihood and SVM are selected to sever as the sub-classifications. The simple vote classification, maximum probability category method and fuzzy integral method are combined together according to certain rules. And adopted color infrared aerial images of Huairen country as the experimental object. The results show that the overall classification accuracy was improved by 12% and Kappa coefficient was increased by 0.12 compared with SVM classification which has the highest accuracy in single sub-classifications.

In this paper anaerobic digestion of dairy manure and straw was conducted to produce biogas. Under the conditions of C/N=25-30 and T=36°C, five kinds of dry matter concentration of 20%, 15%, 10%, 5% and 2.5% were tested to investigate the effect of dry matter concentration on anaerobic digestion. The result showed that first 30 days was the biogas production peak phase and VFA concentrations in the leachate were also high during the same period. When dry matter concentration increased, biogas production appeared larger fluctuation, and alkalinity and NH4+-N concentration in the leachate also increased with higher organic loading rate. Among five kinds of dry matter concentration, 10% was more suitable for anaerobic digestion to produce biogas with total biogas production amount of 4710 mL after 30 days and volumetric biogas yield of 0.313 m3•m-3•d-1. These results could provide instructive meaning to the engineering application of dry anaerobic digestion.

Mechanical properties of Al-Mg porous materials which were made by self melting reactions at low temperatures are investigated in this work. The results showed that the yield strength of Al-20%Mg was up to 240 MPa, and this is the compressive characteristics of brittle porous materials. The excellent of mechanical properties of Al-Mg porous material was analyzed and the reason was ascribed to the homogenous distributed pore with cell wall strengthening.

In this paper, the nosing process of metal tube with a conical die is investigated using the finite element method, and a series of simulations on the tube nosing process by using the program ABAQUS is carried out. The concrete process of tube nosing deformation is described. Some simulation results on tube nosing deformation such as the distributions of the Von Mises stress and effective strain, the material thickness variation of deformation zone are obtained and analyzed.

The ultrasensitive bio-barcode amplification assay (BCA) technique was developed for the specific detection of the outer-core protein VP7 of bluetongue virus (BTV). The target antigen VP7 was first captured by gold nanoparticles (GNPs) coated with polyclonal antibodies. Magnetic microparticles (MMP) coated with VP7 monoclonal antibody were then added to form a sandwich immuno-complex. After the immuno-complex was formed, signal DNA annealed to DNA strands covalently bound to the GNPs were released by heating and characterized by PCR and real-time fluorescence PCR. A detection limit of 0.1 fg/ml was measured for purified VP7, seven orders of magnitude more sensitive than that of conventional antigen capture ELISA. The BCA demonstrated the same enhanced sensitivity for detecting BTV in serum samples from sheep. In the following work it is demonstrated that this assay is a highly sensitive method for the detection of BTV proteins that could be adapted to measure other proteins.

Dry anaerobic co-digestion of animal manure and straw was conducted to produce biogas. Startup characteristics and biogas production perform of dry digestion were studied, and the effect of operation temperature and incubation amount on dry digestion was also investigated. The study result showed that under the conditions of C/N=25-30, TS=20% and T=(36±1) °C,dry digestion can start up quickly with acclimated thickening sludge as incubation sludge. Compared to dry digestion of chicken manure and pig manure, dry digestion of cow manure proceeded steadily with high biogas yield. It is found that incubation is necessary in the process of dry digestion and biogas yield increases with more incubation amount. The appreciate incubation ratio is about 10%. Temperature change has apparent effect on biogas production, and it is suggested that mesophilic temperature should be chosen in the scaled project of dry digestion.

The relationship between formaldehyde emission and time was researched and a mathematical model was developed which describes the variation of formaldehyde with time in the airtight chamber. It was found that high quality composite floor was in line with 0-order kinetic equation and low quality composite floor was in line with 1-order kinetic equation. Besides, the effect of activated carbon adsorption on formaldehyde was studied and the result showed that activated carbon had poor adsorption on formaldehyde for weak van der waals force.

The performance research of Friction Stir Weld’s Joints of Aluminum Alloy under different welding parameters is very necessary and valuable in the engineering. Two points is proposed in this paper. One is the new fracture criterion using the function curve of the value of critical crack opening displacement (|CODc|) as a fracture parameter, the other is using critical crack opening angle (CTOA) as a fracture parameter.

In this paper aerobic process was applied in an anaerobic baffled reactor to form an innovative Hybrid Baffled Reactor (HBR), which was used to investigate the treatment effect of livestock and poultry farming wastewater. Biofilm cultivation, the influence of Hydraulic Retention Time (HRT) and treatment effect in three tanks were also discussed. The study result showed that good COD and NH4+-N removal efficiency was achieved in the reactor. When the influent COD concentration, NH4+-N concentration and HRT were 900 mg/L, 200 mg/L and 24 h, the concentrations of COD and NH4+-N in the outlet were smaller than 100 mg/L and 70 mg/L, and COD removal efficiency and nitrification rate were 90% and 70% respectively.

The strip flatness and crown control model is the foundation of automatic strip shape control. Considering the metal transverse flows and the inter stand second deformation, the trip flatness and crown control model has been developed, which can be applied to CVC mills and PC mills as well as normal four-high mills. The strip flatness and crown control model has high precision, and has been successfully applied to the automatic strip shape control system reconstruction of Tangshan Ganglu 1 250 mm hot strip plant.

Hepatolithiasis is associated with the presence of intrahepatic biliary strictures, and balloon dilatation is the main approach. However, this method is difficult to implement if the bile duct distal to the stricture is blocked by stones. Therefore, alternative methods need to be explored to effectively treat hepatolithiasis. The aim of this study is to investigate the feasibility and effectiveness of choledochoscopic high-frequency needle-knife electrotomy for the treatment of intrahepatic biliary strictures.Clinical data of 58 patients suffering from intrahepatic bile duct strictures from January 2011 to January 2013 were retrospectively analyzed. Choledochoscopic electrotomy was used to resolve the strictures.One hundred thirty-four sites of intrahepatic bile duct strictures were discovered. The average operating time of electrotomy is 5.6 min (range, 1 ∼ 15 min). Structured bile duct tissue bleeding occurred in eight sites (8/134, 6.0%) but were resolved by endoscopic high-frequency electric cautery. After the operations, 14 cases of cholangitis (14/58, 24.1%), three cases of delayed hemobilia, one case of liver abscess (1/58, 1.7%), and seven cases of stenting exodus (7/58, 12.1%) were observed despite conservative treatment and stenting reset. The average supporting time was 7.0 months (6 ∼ 9 months). No abnormal bile duct structure or presence of stone was found according to choledochoscopy. The follow-up period ranged from 12 to 48 months. Hepatolithiasis recurred in five (5/58, 8.6%) patients, and the cumulative recurrent probability of intrahepatic bile duct stricture was 5.2% (7/134).Choledochoscopic high-frequency needle-knife electrotomy could be considered as a simple, safe, and effective complementary approach for treating intrahepatic biliary strictures.

The effects of neomycin, one of the aminoglycoside antibiotics, on the acetylcholine (ACh)-induced current (I(ACh)) were studied in pheochromocytoma cells by using the whole-cell clamp technique. The I(ACh) proved to be generated through neuronal nicotinic receptor. ACh (30 microM) induced an inward current at a holding potential of -80 mV. When cells were treated with neomycin (0.01-1 mM) and ACh (30 microM) simultaneously, an inhibitory effect of neomycin on the peak of I(ACh) was found. This effect was fast, reversible, and concentration dependent. Pretreatment with neomycin for 3-8 min had no effect on the inhibition of I(ACh) induced by neomycin. External application of 0.1 mM neomycin neither shifted the dose-response curve of the peak I(ACh) to the right (dissociation constant (K(d)) = 16.5 microM) nor affected its coefficient (1.8) but inhibited the curve amplitudes by approximately 33%. Stimulated protein kinase C activation by using an exogenous activator produced inhibition of I(ACh), while using protein kinase C inhibitor (PKCI 19-31) had no effect on the inhibition of I(ACh) induced by neomycin. These results suggest that neomycin has an inhibitory effect on I(ACh) without the involvement of phospholipase C. It indicates that neomycin binds to a specific site on the cell membrane, probably on the neuronal nicotinic receptor-coupled channel, and inhibits the I(ACh) in a noncompetitive manner, thus controlling the immediate catecholamine release from the sympathetic cells.

In this study, a previously unknown fungal immunomodulatory protein (FIP), here called FIP-ppl, was identified from the basidiomycete fungus Postia placenta by searching its genome sequence database using known FIPs as baits, which was the first basidiomycete FIP to be identified outside the order of edible macro fungi. The gene FIP-ppl was synthesized and expressed in Escherichia coli to produce a glutathione S-transferase (GST) fusion protein. The fusion protein was purified on a GST affinity column and the protein tag was removed using in situ thrombin cleavage. The purified recombinant protein (rFIP-ppl) displayed hemagglutination activity toward rabbit red blood cells but not against human red blood cells. RFIP-ppl stimulated mouse splenocyte cell proliferation and enhanced interleukin-2 (IL-2) release. Antitumor assays indicated that rFIP-ppl had significant cell proliferation inhibitory activity and apoptotic effects in human tumor cells with more pronounced inhibiting proliferation and inducing apoptotic effects on gastric tumor cells (MGC823) than against hepatoma (HepG2) cells. This study confirms an alternative means of identifying, producing, and isolating new FIPs. It may provide convenient access to FIP-ppl with potential human therapeutic applications.

A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode assay (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In the GNPA, a chain of ricin was captured by a GNP probe coated with polyclonal antibodies and single-stranded signal DNA. A magnetic microparticle (MMP) probe coated with ricin A chain monoclonal antibody was then added to form an immuno-complex. After being magnetically separated, the immuno-complex containing the single-stranded signal DNA was characterized by PCR and real-time PCR. A detection limit of 10(-2) fg/ml was determined for the ricin A chain; this is eight orders of magnitude more sensitive than that achieved with an ELISA and two orders more sensitive than that obtained with the BCA. The coefficients of variation (CV) of the intra- and inter-assay values ranged from 3.82-6.46%. The results here show that this novel assay is an ultrasensitive method for detection of ricin proteins and may be suitable for the ultrasensitive detection of other proteins.

To study the effect of PP333 treatment on seed yield,medicinal materials yield and quality of Flemingia Philippinensis.The experiment was in split plot design for PP333 in main plot and in planting density subplot.The seed yield of 600 mg/L (PP333) + 60 plants/m2 (planting density) was the highest. The medicinal materials yield of 600 mg/L (PP333) + 90 plants/m2 (planting density) was the highest. The quality of 900 mg/L( PP333) + 60 plants/m2 (planting density) was the best.The appropriate density of PP333 and planting can improve seed yield,medicinal materials yield and quality of Flemingia Philippinensis.

When the wind energy is separated from the power grid, explore to find a way by using four-leg active power filter to guarantee power quality to solve the problem caused by four-wire nonlinear load. Considering the disadvantage of phase locked loop, a real-time algorithm of detecting harmonic current based on improved ip-iq theory is proposed. The first step is to remove the zero sequence of the three phase current from the original current. And then in order to gain the compensation instructions of the harmonic current and zero sequence current and reactive current, using mean method of slide time window to get rid of the fundamental active current. And based on these steps, a dynamic and real-time simulation model is set up, under the environment of MATLAB- SIMULINK and the components of the libraries of SimPowSystems. Besides, the model of Fcn is used to program. The simulation results show that the detective algorithm of the harmonic current of nonlinear load is correct.

In order to improve and accelerate the detection of West Nile virus (WNV), a rapid and specific real-time reverse transcription polymerase chain reaction (rtRT-PCR) was established. Primers and probe were designed according to the conservative sequence of capsid protein gene of WNV. Tenfold successive dilutions of positive WNV DNA were used to measure the sensitivity of rtRT-PCR. The amplifying curve showed that this method could successfully amplify 101 copies/μl WNV gene, while reference to Japanese encephalitis virus (JEV) and blank control were all negative. The assay system showed high reproducibility with coefficient of variation (CV) < 2%. The detection of WNV can be completed within 2 to 3 h. By detecting cDNA samples (n = 55) with rtRT-PCR and the conventional PCR assay, the established rtRT-PCR showed 96.36% (37 + 16/55) coincidence rate with the conventional PCR. All the results showed that the newly established rtRT-PCR assay was shown to be a rapid, sensitive and specific test for detecting WNV. Key words: West Nile virus, capsid protein gene, real-time RT-PCR.

The immunostimulatory activity of Sophora flavescens polysaccharide (SFPW1) was evaluated by using in vitro cell models and in vivo animal models. The results demonstrated that SFPW1 could effectively inhibit the tumor growth in H22 tumor-bearing mice and promote the splenocyte proliferation, thus resulting in a prolonged life survival. For assay in vitro, SFPW1 significantly strengthened peritoneal macrophages to devour H22 tumor cells and stimulated macrophages to produce nitric oxide (NO) via up-regulation of inducible NO synthase (iNOS) activity. However, no direct cytotoxicity against H22 tumor cells was observed in vitro. These results suggest that SFPW1 might be a strong natural immunomodulator and the antitumor effect of this polysaccharide is associated with its potent immunostimulating effect.

Rapid, reliable and quantitative assays of virus universal detection are essential for fighting against Canine Parvovirus (CPV). A rapid fluorescence probe-based real-time polymerase chain reaction (RT-PCR) for detecting Canine Parvovirus was established. Primers were designed according to VP2 protein gene by Primer Premier 5.0. In response, a sensitive assay with the TaqMan probe was developed and validated. Ten-fold successive dilutions of positive CPV DNA plasmids were used to measure the sensitivity of (RT-PCR). Amplifying curve showed that this method could successfully amplify 102 copies/μL CPV gene, meanwhile reference Porcine Parvovirus (PPV), Porcine Circovirus (PCV) and blank control were all negative. The assay system showed high reproducibility with CV

Arbuscular mycorrhizal (AM) fungi, an important component of soil microbes, are of significance in maintaining the sustainable development of urban ecosystem. This paper summarized the characteristics of the colonization and community structure of AM fungi in urban ecosystems, and discussed the effects of urban ecological factors, e.g., human activities, vegetation re-establishment and maintenance, and urban soil status, on the colonization and community structure. It was considered that the researches on the community structure and function of AM fungi in urban ecosystems, such as the effects and mechanisms of the key urban ecological factors (e.g., water resource shortage and heat island effect) on the alternation of AM fungal community structure should be strengthened in the future.

Materials of aluminum alloy, a kind of lightweight material, were employed widely in the field of engineering. For the research of friction stir weld's joints of aluminum alloy under different welding parameters, It is found that the value of critical crack opening displacement (|CODcritc|) is constant when stable crack growth and hereby tried to suggest that the |CODcritc| as a fracture parameter to establish one fracture criterion in order to optimize the welding parameters. But the |CODcritc| is variable on the initial stage of unstable crack growth, if the constant on the stable stage was introduced in the simulation to analyze the whole course of the crack development, the results of the simulation would be not accuracy enough. Therefore, the other criterion, the introduction of the function curve of |CODcritc|, was proposed and could solve the related problems very well. Using the software of Franc2D/L to build the Arcan model of Aluminum Alloy 2524-T351 (short in AA2524), compare the results of simulation to the experiment and also compare the results from two criterions.

The properties and sensitivity to acetylcholine of PC12 cells differentiated with nerve growth factor (NGF) have been investigated by using whole-cell clamp technique.When cultured in the presence of NGF, PC12 cells not only differentiated to resemble sympathetic neurons morphologically, but also developed electrical excitability. NGF-treated PC12 cells were highly sensitive to ACh than untreated cells. The I(Ach) proved to be generated by nAChR by pharmacological identification. Nicotinic receptor was characterized by desensitization. The macroscopic I(ACh) was inward rectified and concentration dependent.PC12 cells are easily cultured and provides a homogenous population of cells. When culture in NGF, they differentiate to sympathetic-like neurons that contain on their surface neuronal nAChR, it can be used as good model system for studying regulation of a sympathetic neuronal nAChR.

Twenty-four serotypes of bluetongue virus (BTV) have been recognized world wide. Reliable and quantitative assays of virus universal detection are essential for fighting against BT. A real-time reverse transcription-polymerase chain reaction (RT-PCR) with a TaqMan fluorescence probe has been developed for detection of the NS1 gene of different BTV serotypes. In BHK-21 cells, in the assay detected BTV1-22 specifically, and had no cross-reactivity with the closely related epizootic hemorrhagic disease virus (EHDV) serotypes 1-5. The limit of sensitivity of the assay was 0.1 TCID(50)/ml for BTV-1 and 10(2) copies for the control R121/pGEM. Accurate quantitation can be achieved with samples containing between 10(2) and 10(6) copies. The coefficient of variation (CV) of intra-assay and inter-assay ranged from 2.17% to 5.60%. The developed real-time RT-PCR assay showed good coincident rate (99.2%) with duplex RT-PCR in 122 whole blood clinical samples from sheep. Therefore, the real-time RT-PCR can be a reliable method for detection of various serotypes of BTV.