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Additional file 1. Dynamic changes of complete blood counts and immune parameters pre-RT and post-RT

Additional file 1. Table: Univariate and multivariate analysis of dosimetric parameters associated with the risk of ncRILD.

Novel hydrophilic NaYF4:Yb3+,Tm3+@NaGdF4:Ce3+,Eu3+ double-jacket microtubes (DJMTs) with upconversion/ downconversion dual-mode luminescence were designed and prepared through epitaxial growth of NaGdF4:Ce3+, Eu3+ shell onto the NaYF4:Yb3+,Tm3+ microtube via poly(acrylic acid) (PAA) mediated hydrothermal method. It is demonstrated that PAA ligand played an important role in guiding the direct growth of NaGdF4:Ce3+,Eu3+ shell onto the surface of NaYF4:Yb3+,Tm3+ parent microtubes. The growth of NaGdF4:Ce3+,Eu3+ shell experienced a crystal phase transition from β-NaGdF4 and β-NaYF4 mixture to β-NaYF4@NaGdF4 composite crystal, and morphology evolution from mixture of β-NaGdF4:Ce3+,Eu3+ nanorods and β-NaYF4:Yb3+,Tm3+ microtubes to NaYF4:Yb3+,Tm3+@NaGdF4: Ce3+,Eu3+ DJMTs. The formation mechanism of DJMTs was the dissolution−renucleation of β-NaGdF4:Ce3+,Eu3+ nanorods and the growth of β-NaGdF4:Ce3+,Eu3+ shell via the classical Ostwald ripening mechanism. The as-prepared DJMTs could exhibit blue upconversion and red downconversion luminescence, which was further made into environmentally benign luminescent inks for creating highly secured and fluorescent-based anti-counterfeiting patterns via inkjet printing.

A computational hemodynamics method was employed to investigate how the morphotype and functional state of aortic valve would affect the characteristics of blood flow in aortas with pathological dilation, especially the intensity and distribution of flow turbulence. Two patient-specific aortas diagnosed to have pathological dilation of the ascending segment while differential aortic valve conditions (i.e., one with a stenotic and regurgitant RL bicuspid aortic valve (RL-BAV), whereas the other with a quasi-normal tricuspid aortic valve (TAV)) were studied. When building the computational models, in addition to in vivo data-based reconstruction of geometrical model and boundary condition setting, the large eddy simulation method was adopted to quantify potential flow turbulence in the aortas. Obtained results revealed the presence of complex flow patterns (denoted by time-varying changes in vortex structure), flow turbulence (indicated by high turbulent eddy viscosity (TEV)), and regional high wall shear stress (WSS) in the ascending segment of both aortas. Such hemodynamic characteristics were significantly augmented in the aorta with RL-BAV. For instance, the space-averaged TEV in late systole and the wall area exposed to high time-averaged WSS (judged by WSS> two times of the mean WSS in the entire aorta) in the ascending aortic segment were increased by 176% and 465%, respectively. Relatively, flow patterns in the descending aortic segment were less influenced by the aortic valve disease. These results indicate that aortic valve disease has profound impacts on flow characteristics in the ascending aorta, especially the distribution and degree of high WSS and flow turbulence.

Background: Methyltransferase-like 3(METTL3)-mediated N6-methyladenosine (m6A) modification has been reported to regulate microRNAs maturation. Here, the study was designed to investigate the regulatory effect of m6A-dependent miRNA maturation on pancreatic cancer progression which is still limited before.Results: We found that METTL3 significantly upregulated in the pancreatic tumor tissues. Overexpression of METTL3 promoted cancer cell proliferation and migration in vitro and tumor progression in vivo. METTL3-mediated m6A modification facilitated miR-196a maturation in pancreatic cancer cells, and miR-196a increased the proliferation and migration of cancer cells in vitro. Luciferase reporter assay verified that cytoplasmic polyadenylation element binding protein 3 (CPEB3) was a direct target gene of miR-196a. In vivo studies proved that overexpression of miR-196a inhibited the anti-tumor effect of knockdown of METTL3, and overexpression of CPEB3 inhibited the miR-196a-enhanced tumor progression. Conclusions: We identified that METTL3 was upregulated in pancreatic cancer, leading to the upregulation of miR-196a, resulting in the downregulation of CPEB3, which promoted the pancreatic tumor progression. We first demonstrated that CPEB3 was a tumor suppressor gene in pancreatic cancer, and the METTL3 regulated miR-196a/CPEB3 axis may be a therapeutic target for pancreatic cancer therapy.

In order to optimize the hot deformation processing, useful and efficient mathematical models able to evaluate the different aspects of the response of plain carbon steel to hot plastic deformation are among the most important prerequisites. In this paper, a group of mathematical equations were developed according to a series of experiments data to model the austenite grain evolution, static and dynamic recrystallization during-deformation and post-deformation of plain carbon steel. And then those equations were implemented into ABAQUS6.5 (License Agreement Identifier: 27SHANU) using the VUMAT for numerical simulations of cylinder specimen compression.

In hot metal forming processes, the material is subjected to the thermo-mechanical processing. A fully three dimensional thermo-mechanically coupled FEM-simulation of an eleven pass hot rough rolling process of H-beam has been performed. Microstructure evolution equations available in literatures were incorporated into the commercial FE solver ABAQUS/Explicit, through user defined subroutine VUMAT, to simulate the microstructure evolution. Since it’s impractical to obtain the austenite grain size distribution in the beam blank during industrial hot rolling, the calculated rolling loads are compared with the mills loads instead of grain size comparison between the predicted average value and the real ones.

Strain ZSI isolated from heavy metal contaminated soil was identified by morphological observation and 16S rDNA sequence analysis. The tolerance to heavy metals and 12 antibiotics was examined. The results showed that the strain was most homophylic with Cupriavidus sp. It has high tolerance to serval metals, and the order was Cu＞Zn＞Cd＞Ni＞Mn=Co＞Pb. The strain can resistant to 9 antibiotics.

Hot rolling is a basic metal forming technique that is used to transform preformed shape into final products of forms more suitable for further processing. As the hot stock progresses through the forming surfaces, the shape eventually reaches a constant state. With the assumption of forming process has reached steady-state condition, a simulation technique based on elements re-meshes has been constructed to analyze the H-beam metal hot roll process. The technique includes the following approaches: the solution was halted as soon as the steady-state criteria were met, and the plane of elements, which first satisfied the steady-state criteria were written to database, SSES for short; a two-dimensional model was created to model the hot stock cooling between the two roll passes and a geometric part was generated and meshed with quadrilateral elements to transfer the nodes temperatures; a new three-dimensional model extruded from the two-dimensional model was constructed to model the next roll pass with the transfer of nodes temperatures and element integration points equivalent plastic strain(PEEQ), identifying the plastic deformation extent for the classical metal plasticity models, from the new two-dimensional model and the first three-dimensional model respectively. Gleeble-1500 tester is used to get the true stress and true plastic strain data for modeling the yield behavior of material Q235. The effectiveness of the simulation technique has been proved by a simulation of 11-pass H-beam rolling process.

Beauvericin (BEA) is a cyclic hexadepsipeptide mycotoxin with notable phytotoxic and insecticidal activities. Fusarium redolens Dzf2 is a highly BEA-producing fungus isolated from a medicinal plant. The aim of the current study was to develop a simple and valid kinetic model for F. redolens Dzf2 mycelial growth and the optimal fed-batch operation for efficient BEA production. A modified Monod model with substrate (glucose) and product (BEA) inhibition was constructed based on the culture characteristics of F. redolens Dzf2 mycelia in a liquid medium. Model parameters were derived by simulation of the experimental data from batch culture. The model fitted closely with the experimental data over 20-50 g l(-1) glucose concentration range in batch fermentation. The kinetic model together with the stoichiometric relationships for biomass, substrate and product was applied to predict the optimal feeding scheme for fed-batch fermentation, leading to 54% higher BEA yield (299 mg l(-1)) than in the batch culture (194 mg l(-1)). The modified Monod model incorporating substrate and product inhibition was proven adequate for describing the growth kinetics of F. redolens Dzf2 mycelial culture at suitable but not excessive initial glucose levels in batch and fed-batch cultures.

Fusarium redolens Dzf2, an endophytic fungal species, is a high producer of the antibiotic compound beauvericin (BEA). However, the BEA produced by the F. redolens Dzf2 fungus was retained mainly as an intracellular product. This study was to evaluate an integrated fermentation-in situ product recovery process for enhancement of BEA production in F. redolens Dzf2 myelical culture. A macroporous polystyrene resin (X-5) was selected as the sorbent and added to the mycelial culture flasks (enclosed in a nylon bag). With 2 g resin added to 40 ml medium in each flask in the early stationary growth phase (day 5), the volumetric BEA yield (on day 7) was increased from 194 to 265 mg l −1 , with 65% being adsorbed to the resin phase. With resin renewal plus glucose feeding (on day 7), the BEA production was increased even more dramatically to 400 mg l −1 (on day 9), double of the yield in the batch control culture. The results show that in situ adsorption was an effective strategy for enhancing the BEA production and also facilitating its recovery in the mycelial liquid culture.