576 results on '"Li DQ"'
Search Results
2. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gomez, E, Alessandri, C, Ali, M, Al-Bari, MAA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Alves, S, da Costa, CA, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, ZY, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Anton, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araujo, WL, Araya, J, Arden, C, Arevalo, MA, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnaune-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Avalos, Y, Aveic, S, Aveleira, CA, AvinWittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, DH, Bae, ON, Bae, SH, Baehrecke, EH, Baek, A, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, XY, Bai, YD, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barila, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Jr, RCB, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touze, N, Bellarosa, C, Belleudi, F, Perez, MB, Bello-Morales, R, Beltran, JSD, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, LR, Bi, XL, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, BoeszeBattaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, Bordi, M, Borner, C, Bornhauser, BC, Borthakur, G, Bosch, J, Bose, S, Botana, LM, Botas, J, Boulanger, CM, Boulton, ME, Bourdenx, M, Bourgeois, B, Bourke, NM, Bousquet, G, Boya, P, Bozhkov, PV, Bozi, LHM, Bozkurt, TO, Brackney, DE, Brandts, CH, Braun, RJ, Braus, GH, Bravo-Sagua, R, Bravo-San Pedro, JM, Brest, P, Bringer, MA, Briones-Herrera, A, Broaddus, VC, Brodersen, P, Alvarez, EMC, Brodsky, JL, Brody, SL, Bronson, PG, Bronstein, JM, Brown, CN, Brown, RE, Brum, PC, Brumell, JH, Brunetti-Pierri, N, Bruno, D, Bryson-Richardson, RJ, Bucci, C, Buchrieser, C, Bueno, M, Buitrago-Molina, LE, Buraschi, S, Buch, S, Buchan, JR, Buckingham, EM, Budak, H, Budini, M, Bultynck, G, Burada, F, Burgoyne, JR, Buron, MI, Bustos, V, Buttner, S, Butturini, E, Byrd, A, Cabas, I, Cabrera-Benitez, S, Cadwell, K, Cai, JJ, Cai, L, Cai, Q, Cairo, M, Calbet, JA, Caldwell, GA, Caldwell, KA, Call, JA, Calvani, R, Calvo, AC, Barrera, MCR, Camara, NO, Camonis, JH, Camougrand, N, Campanella, M, Campbell, EM, Campbell-Valois, FX, Campello, S, Campesi, I, Campos, JC, 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De Franceschi, L, De Leonibus, C, Barbosa, MGD, De Meyer, GRY, De Milito, A, De Nunzio, C, De Palma, C, De Santi, M, De Virgilio, C, De Zio, D, Debnath, J, DeBosch, BJ, Decuypere, J, Deehan, MA, Deflorian, G, DeGregori, J, Dehay, B, Del Rio, G, Delaney, JR, Delbridge, LMD, Delorme-Axford, E, Delpino, MV, Demarchi, F, Dembitz, V, Demers, ND, Deng, HB, Deng, ZQ, Dengjel, J, Dent, P, Denton, D, DePamphilis, ML, Der, CJ, Deretic, V, Descoteaux, A, Devis, L, Devkota, S, Devuyst, O, Dewson, G, Dharmasivam, M, Dhiman, R, di Bernardo, D, Di Cristina, M, Di Domenico, F, Di Fazio, P, Di Fonzo, A, Di Guardo, G, Di Guglielmo, GM, Di Leo, L, Di Malta, C, Di Nardo, A, Di Rienzo, M, Di Sano, F, Diallinas, G, Diao, JJ, Diaz-Araya, G, Diaz-Laviada, I, Dickinson, JM, Diederich, M, Dieude, M, Dikic, I, Ding, SP, Ding, WX, Dini, L, Dinic, M, Dinkova-Kostova, AT, Dionne, MS, Distler, JHW, Diwan, A, Dixon, IMC, Djavaheri-Mergny, M, Dobrinski, I, Dobrovinskaya, O, Dobrowolski, R, Dobson, RCJ, Emre, SD, Donadelli, M, Dong, B, Dong, XN, Dong, ZW, Ii, GWD, Dotsch, V, Dou, H, Dou, J, Dowaidar, M, Dridi, S, Drucker, L, Du, AL, Du, CG, Du, GW, Du, HN, Du, LL, du Toit, A, Duan, SB, Duan, XQ, Duarte, SP, Dubrovska, A, Dunlop, EA, Dupont, N, Duran, RV, Dwarakanath, BS, Dyshlovoy, SA, Ebrahimi-Fakhari, D, Eckhart, L, Edelstein, CL, Efferth, T, Eftekharpour, E, Eichinger, L, Eid, N, Eisenberg, T, Eissa, NT, Eissa, S, Ejarque, M, El Andaloussi, A, El-Hage, N, El-Naggar, S, Eleuteri, AM, El-Shafey, ES, Elgendy, M, Eliopoulos, AG, Elizalde, MM, Elks, PM, Elsasser, HP, Elsherbiny, ES, Emerling, BM, Emre, NCT, Eng, CH, Engedal, N, Engelbrecht, AM, Engelsen, AST, Enserink, JM, Escalante, R, Esclatine, A, Escobar-Henriques, M, Eskelinen, EL, Espert, L, Eusebio, MO, Fabrias, G, Fabrizi, C, Facchiano, A, Facchiano, F, Fadeel, B, Fader, C, Faesen, AC, Fairlie, WD, Falco, A, Falkenburger, BH, Fan, DP, Fan, J, Fan, YB, Fang, EF, Fang, YS, Fang, YQ, Fanto, M, Farfel-Becker, T, Faure, M, Fazeli, G, Fedele, AO, Feldman, AM, Feng, D, Feng, JC, Feng, LF, Feng, YB, Feng, YC, Feng, W, Araujo, TF, Ferguson, TA, Fernandez-Checa, JC, FernandezVeledo, S, Fernie, AR, Ferrante, AW, Ferraresi, A, Ferrari, MF, Ferreira, JCB, Ferro-Novick, S, Figueras, A, Filadi, R, Filigheddu, N, FilippiChiela, E, Filomeni, G, Fimia, GM, Fineschi, V, Finetti, F, Finkbeiner, S, Fisher, EA, Fisher, PB, Flamigni, F, Fliesler, SJ, Flo, TH, Florance, I, Florey, O, Florio, T, Fodor, E, Follo, C, Fon, EA, Forlino, A, Fornai, F, Fortini, P, Fracassi, A, Fraldi, A, Franco, B, Franco, R, Franconi, F, Frankel, LB, Friedman, SL, Frohlich, LF, Fruhbeck, G, Fuentes, JM, Fujiki, Y, Fujita, N, Fujiwara, Y, Fukuda, M, Fulda, S, Furic, L, Furuya, N, Fusco, C, Gack, MU, Gaffke, L, Galadari, S, Galasso, A, Galindo, MF, Kankanamalage, SG, Galluzzi, L, Galy, V, Gammoh, N, Gan, BY, Ganley, IG, Gao, F, Gao, H, Gao, MH, Gao, P, Gao, SJ, Gao, WT, Gao, XB, Garcera, A, Garcia, MN, Garcia, VE, Garcia-Del Portillo, F, Garcia-Escudero, V, GarciaGarcia, A, Garcia-Macia, M, Garcia-Moreno, D, Garcia-Ruiz, C, Garcia-Sanz, P, Garg, AD, Gargini, R, Garofalo, T, Garry, RF, Gassen, NC, Gatica, D, Ge, L, Ge, WZ, Geiss-Friedlander, R, Gelfi, C, Genschik, P, Gentle, IE, Gerbino, V, Gerhardt, C, Germain, K, Germain, M, Gewirtz, DA, Afshar, EG, Ghavami, S, Ghigo, A, Ghosh, M, Giamas, G, Giampietri, C, Giatromanolaki, A, Gibson, GE, Gibson, SB, Ginet, V, Giniger, E, Giorgi, C, Girao, H, Girardin, SE, Giridharan, M, Giuliano, S, Giulivi, C, Giuriato, S, Giustiniani, J, Gluschko, A, Goder, V, Goginashvili, A, Golab, J, Goldstone, DC, Golebiewska, A, Gomes, LR, Gomez, R, Gomez-Sanchez, R, Gomez-Puerto, MC, Gomez-Sintes, R, Gong, Q, Goni, FM, Gonzalez-Gallego, J, Gonzalez-Hernandez, T, Gonzalez-Polo, RA, Gonzalez-Reyes, JA, Gonzalez-Rodriguez, P, Goping, IS, Gorbatyuk, MS, Gorbunov, NV, Gorojod, RM, Gorski, SM, Goruppi, S, Gotor, C, Gottlieb, RA, Gozes, I, Gozuacik, D, Graef, M, Graler, MH, Granatiero, V, Grasso, D, Gray, JP, Green, DR, 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VN, Vaccari, T, Vaccaro, MI, Vahsen, BF, Vakifahmetoglu-Norberg, H, Valdor, R, Valente, MJ, Valko, A, Vallee, RB, Valverde, AM, Van den Berghe, G, van Der Veen, S, Van Kaer, L, van Loosdregt, J, van Wijk, SJL, Vandenberghe, W, Vanhorebeek, I, Vannier-Santos, MA, Vannini, N, Vanrell, MC, Vantaggiato, C, Varano, G, Varela-Nieto, I, Varga, M, Vasconcelos, MH, Vats, S, Vavvas, DG, VegaNaredo, I, Vega-Rubin-de-Celis, S, Velasco, G, Velazquez, AP, Vellai, T, Vellenga, E, Velotti, F, Verdier, M, Verginis, P, Vergne, I, Verkade, P, Verma, M, Verstreken, P, Vervliet, T, Vervoorts, J, Vessoni, AT, Victor, VM, Vidal, M, Vidoni, C, Vieira, OV, Vierstra, RD, Vigano, S, Vihinen, H, Vijayan, V, Vila, M, Vilar, M, Villalba, JM, Villalobo, A, Villarejo-Zori, B, Villarroya, F, Villarroya, J, Vincent, O, Vindis, C, Viret, C, Viscomi, MT, Visnjic, D, Vitale, I, Vocadlo, DJ, Voitsekhovskaja, OV, Volonte, C, Volta, M, Vomero, M, Von Haefen, C, Vooijs, MA, Voos, W, Vucicevic, L, Wade-Martins, R, Waguri, S, Waite, KA, Wakatsuki, S, Walker, DW, Walker, MJ, Walker, SA, Walter, J, Wandosell, FG, Wang, B, Wang, CY, Wang, C, Wang, CR, Wang, CW, Wang, D, Wang, FY, Wang, F, Wang, FM, Wang, GS, Wang, H, Wang, HX, Wang, HG, Wang, JR, Wang, JG, Wang, J, Wang, JD, Wang, K, Wang, LR, Wang, LM, Wang, MH, Wang, MQ, Wang, NB, Wang, PW, Wang, PP, Wang, P, Wang, QJ, Wang, Q, Wang, QK, Wang, QA, Wang, WT, Wang, WY, Wang, XN, Wang, XJ, Wang, Y, Wang, YC, Wang, YZ, Wang, YY, Wang, YH, Wang, YP, Wang, YQ, Wang, Z, Wang, ZY, Wang, ZG, Warnes, G, Warnsmann, V, Watada, H, Watanabe, E, Watchon, M, Weaver, TE, Wegrzyn, G, Wehman, AM, Wei, HF, Wei, L, Wei, TT, Wei, YJ, Weiergraber, OH, Weihl, CC, Weindl, G, Weiskirchen, R, Wells, A, Wen, RXH, Wen, X, Werner, A, Weykopf, B, Wheatley, SP, Whitton, JL, Whitworth, AJ, Wiktorska, K, Wildenberg, ME, Wileman, T, Wilkinson, S, Willbold, D, Williams, B, Williams, RSB, Williams, RL, Williamson, PR, Wilson, RA, Winner, B, Winsor, NJ, Witkin, SS, Wodrich, H, Woehlbier, U, Wollert, T, Wong, E, Wong, JH, Wong, RW, Wong, VKW, Wong, WWL, Wu, AG, Wu, CB, Wu, J, Wu, JF, Wu, KK, Wu, M, Wu, SY, Wu, SZ, Wu, SF, Wu, WKK, Wu, XH, Wu, XQ, Wu, YW, Wu, YH, Xavier, RJ, Xia, HG, Xia, LX, Xia, ZY, Xiang, G, Xiang, J, Xiang, ML, Xiang, W, Xiao, B, Xiao, GZ, Xiao, HY, Xiao, HT, Xiao, J, Xiao, L, Xiao, S, Xiao, Y, Xie, BM, Xie, CM, Xie, M, Xie, YX, Xie, ZP, Xie, ZL, Xilouri, M, Xu, CF, Xu, E, Xu, HX, Xu, J, Xu, JR, Xu, L, Xu, WW, Xu, XL, Xue, Y, Yakhine-Diop, SMS, Yamaguchi, M, Yamaguchi, O, Yamamoto, A, Yamashina, S, Yan, SM, Yan, SJ, Yan, Z, Yanagi, Y, Yang, CB, Yang, DS, Yang, H, Yang, HT, Yang, JM, Yang, J, Yang, JY, Yang, L, Yang, M, Yang, PM, Yang, Q, Yang, S, Yang, SF, Yang, WN, Yang, WY, Yang, XY, Yang, XS, Yang, Y, Yao, HH, Yao, SG, Yao, XQ, Yao, YG, Yao, YM, Yasui, T, Yazdankhah, M, Yen, PM, Yi, C, Yin, XM, Yin, YH, Yin, ZY, Ying, MD, Ying, Z, Yip, CK, Yiu, SPT, Yoo, YH, Yoshida, K, Yoshii, SR, Yoshimori, T, Yousefi, B, Yu, BX, Yu, HY, Yu, J, Yu, L, Yu, ML, Yu, SW, Yu, VC, Yu, WH, Yu, ZP, Yu, Z, Yuan, JY, Yuan, LQ, Yuan, SL, Yuan, SSF, Yuan, YG, Yuan, ZQ, Yue, JB, Yue, ZY, Yun, J, Yung, RL, Zacks, DN, Zaffagnini, G, Zambelli, VO, Zanella, I, Zang, QS, Zanivan, S, Zappavigna, S, Zaragoza, P, Zarbalis, KS, Zarebkohan, A, Zarrouk, A, Zeitlin, SO, Zeng, JL, Zeng, JD, Zerovnik, E, Zhan, LX, Zhang, B, Zhang, DD, Zhang, HL, Zhang, H, Zhang, HH, Zhang, HF, Zhang, HY, Zhang, JB, Zhang, JH, Zhang, JP, Zhang, KLYB, Zhang, LSW, Zhang, L, Zhang, LS, Zhang, LY, Zhang, MH, Zhang, P, Zhang, S, Zhang, W, Zhang, XN, Zhang, XW, Zhang, XL, Zhang, XY, Zhang, X, Zhang, XX, Zhang, XD, Zhang, Y, Zhang, YJ, Zhang, YD, Zhang, YM, Zhang, YY, Zhang, YC, Zhang, Z, Zhang, ZG, Zhang, ZB, Zhang, ZH, Zhang, ZY, Zhang, ZL, Zhao, HB, Zhao, L, Zhao, S, Zhao, TB, Zhao, XF, Zhao, Y, Zhao, YC, Zhao, YL, Zhao, YT, Zheng, GP, Zheng, K, Zheng, L, Zheng, SZ, Zheng, XL, Zheng, Y, Zheng, ZG, Zhivotovsky, B, Zhong, Q, Zhou, A, Zhou, B, Zhou, CF, Zhou, G, Zhou, H, Zhou, HB, Zhou, J, Zhou, JY, Zhou, KL, Zhou, RJ, Zhou, XJ, Zhou, YS, Zhou, YH, Zhou, YB, Zhou, ZY, Zhou, Z, Zhu, BL, Zhu, CL, Zhu, GQ, Zhu, HN, Zhu, HX, Zhu, H, Zhu, WG, Zhu, YP, Zhu, YS, Zhuang, HX, Zhuang, XH, Zientara-Rytter, K, Zimmermann, CM, Ziviani, E, Zoladek, T, Zong, WX, Zorov, DB, Zorzano, A, Zou, WP, Zou, Z, Zou, ZZ, Zuryn, S, Zwerschke, W, Brand-Saberi, B, Dong, XC, Kenchappa, CS, Li, ZG, Lin, Y, Oshima, S, Rong, YG, Sluimer, JC, Stallings, CL, and Tong, CK
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flux ,macroautophagy ,phagophore ,stress ,vacuole ,Autophagosome ,LC3 ,lysosome ,neurodegeneration ,cancer - Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
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- 2021
3. A hyaluronan hydrogel scaffold-based xeno-free culture system for ex vivo expansion of human corneal epithelial stem cells
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Stephen C. Pflugfelder, Zuguo Liu, Ding Chen, Li Dq, Zhang L, Qu Y, and Hua X
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0301 basic medicine ,Male ,medicine.medical_specialty ,Cell Culture Techniques ,Limbus Corneae ,Stem cell marker ,Real-Time Polymerase Chain Reaction ,Hydrogel, Polyethylene Glycol Dimethacrylate ,03 medical and health sciences ,Cytokeratin ,0302 clinical medicine ,Ophthalmology ,Laboratory Study ,Medicine ,Humans ,Hyaluronic Acid ,Protein Precursors ,Involucrin ,Cells, Cultured ,Corneal epithelium ,Aged ,Tissue Scaffolds ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,Epithelium, Corneal ,Keratin-12 ,Middle Aged ,Molecular biology ,Epithelium ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Connexin 43 ,Culture Media, Conditioned ,030221 ophthalmology & optometry ,RNA ,Female ,Stem cell ,business ,Fetal bovine serum ,Ex vivo - Abstract
PurposeTo develop a hyaluronan hydrogel scaffold-based xeno-free culture system for ex vivo cultivation of human corneal epithelial stem cells (CESCs).Patients and MethodsCESCs were cultivated from donor limbal explants on the HyStem-C Hydrogel bio-scaffold in 12-well plates for 3 weeks. Group A used the traditional supplemented hormonal epidermal medium (SHEM) and group B used the defined SHEM (without fetal bovine serum and toxin A, adding 20% serum replacement). The growth and morphology of the cultured cells were assessed by phase contrast microscope. The expressions of specific cell markers were assessed by immunofluorescence staining and quantitative real-time PCR (qRT-PCR).ResultsSuccessful cultures of CESCs were obtained in both groups, resulting in multilayered stratified epithelia. Comparing to group A, the cells in group B was grown slightly slower and formed less cellular layers at the end of culture. The corneal specific cytokeratin (K) 12 and differentiation markers, involucrin, and connexin 43, were mainly expressed in the superficial cellular layers in both groups. Interestingly, certain basal cells were immune-positive to proposed stem cell markers such as K19, ABCG2, and integrin β1 in both groups. There was no significant difference between the two groups with regard to the gene expression levels of all these selected corneal markers (all P>0.05).ConclusionsThe hyaluronan hydrogel scaffold-based xeno-free culture system may support the expansion of regenerative CESCs without the risk of xeno component contamination. The regenerated epithelium maintains similar characteristics of native corneal epithelium.
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- 2017
4. Regulated insulin release requires chloride channel 3 (ClC3) for proper granule acidification and priming
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Li, DQ, Jing, XJ, Maritzen, T, Salehi, AS, Lundquist, I, Jentsch, TJ, Rorsman, P, and Renstrom, E
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- 2016
5. PAKs in Human Cancer Progression
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Rakesh Kumar and Li Dq
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0301 basic medicine ,DNA repair ,Cancer ,Context (language use) ,macromolecular substances ,Biology ,equipment and supplies ,medicine.disease ,medicine.disease_cause ,Bioinformatics ,environment and public health ,enzymes and coenzymes (carbohydrates) ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Breast cancer ,PAK1 ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,biological phenomena, cell phenomena, and immunity ,Signal transduction ,p21-activated kinases ,Carcinogenesis - Abstract
Since the initial recognition of a mechanistic role of p21-activated kinase 1 (PAK1) in breast cancer invasion, PAK1 has emerged as one of the widely overexpressed or hyperactivated kinases in human cancer at-large, allowing the PAK family to make in-roads in cancer biology, tumorigenesis, and cancer therapeutics. Much of our current understanding of the PAK family in cancer progression relates to a central role of the PAK family in the integration of cancer-promoting signals from cell membrane receptors as well as function as a key nexus-modifier of complex, cytoplasmic signaling network. Another core aspect of PAK signaling that highlights its importance in cancer progression is through PAK's central role in the cross talk with signaling and interacting proteins, as well as PAK's position as a key player in the phosphorylation of effector substrates to engage downstream components that ultimately leads to the development cancerous phenotypes. Here we provide a comprehensive review of the recent advances in PAK cancer research and its downstream substrates in the context of invasion, nuclear signaling and localization, gene expression, and DNA damage response. We discuss how a deeper understanding of PAK1's pathobiology over the years has widened research interest to the PAK family and human cancer, and positioning the PAK family as a promising cancer therapeutic target either alone or in combination with other therapies. With many landmark findings and leaps in the progress of PAK cancer research since the infancy of this field nearly 20 years ago, we also discuss postulated advances in the coming decade as the PAK family continues to shape the future of oncobiology.
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- 2016
6. Screening of tissues pooled cDNA library using probes by restricted fragments of BAC positive clones of ovine MHC
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Ma Rl, Hugh Tb, Gao Jf, Li Dq, Qiu W, Chen F, Dong Hq, Bai Dz, and Yang Xl
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Genetics ,Bacterial artificial chromosome ,cDNA library ,Sequence analysis ,Complementary DNA ,Genomic library ,General Medicine ,Gene Annotation ,Biology ,Gene ,Genome - Abstract
Under the premise what we have known bacterial artificial chromosome(BAC)clone sequence information and gene annotation predicted in the Chinese Merino sheep major histocompatibility complex (MHC) region, the digested fragments from 6 BAC clones that were located in the MHC region of the Chinese Merino sheep genome BAC library, which were used to screen the cDNA library using plaque in situ hybridization as probes. The full length of positive cDNA clones (sequences) isolated were completely sequenced, and the sequences obtained were aligned with the corresponding known sequence information and the BAC clones with gene annotation. Meanwhile, the sequence similarity was searched in NCBI Blastn database. This work aimed at verification of accuracy of the gene annotation results and initial analysis of gene (sequence) function. At last, 27 positive cDNA clones (sequences) in total were screened through two runs of hybridization. It was also found that these sequences could be positioned in the corresponding BAC clones, and 25 sequences were located in exon area of the annotated gene. It was verified that 23 sequences had the highest sequence similarity with those in the Bos taurus by searching against the NCBI Blastn database; moreover, the function of these sequences were closely relate to immunology.
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- 2012
7. Comparative study of clinicopathological significance, BIRC7, and STC2 expression between squamous cell/adenosquamous carcinomas and adenocarcinoma of gallbladder
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Li Dq, Liang Lf, Zeng Gx, Zou Q, Yang Zl, Yuan Y, Chen Sl, and Li Jh
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Adenosquamous carcinoma ,Adenocarcinoma ,Gastroenterology ,Metastasis ,Inhibitor of Apoptosis Proteins ,Immunoenzyme Techniques ,Carcinoma, Adenosquamous ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Gallbladder cancer ,Stage (cooking) ,Pathological ,Adaptor Proteins, Signal Transducing ,Aged ,Glycoproteins ,Neoplasm Staging ,Aged, 80 and over ,business.industry ,Gallbladder ,Middle Aged ,medicine.disease ,Prognosis ,Neoplasm Proteins ,Survival Rate ,medicine.anatomical_structure ,Oncology ,Lymphatic Metastasis ,Carcinoma, Squamous Cell ,Immunohistochemistry ,Intercellular Signaling Peptides and Proteins ,Female ,Gallbladder Neoplasms ,Neoplasm Recurrence, Local ,business ,Follow-Up Studies - Abstract
Background The differences in clinical, pathological, and biological characteristics between adenocarcinoma (AC) and squamous cell/adenosquamous carcinoma (SC/ASC) of gallbladder cancer have not been well documented. This study is to compare the clinicopathological characteristics and FGFBP1 and WISP-2 expression between AC and SC/ ASC patients. Methods We examined FGFBP1 and WISP-2 expression in 46 SC/ASC and 80 AC samples using immunohistochemistry and analyzed their correlations with clinicopathological characteristics. Results SC/ASCs occur more frequently in older patients and often correspond to larger tumor masses than ACs. Positive FGFBP1 and negative WISP-2 expression were significantly associated with lymph node metastasis and invasion of SC/ASCs and ACs. In addition, positive FGFBP1 and negative WISP-2 expression were significantly associated with differentiation and TMN stage in ACs. Univariate Kaplan‐Meier analysis showed that either elevated FGFBP1 (p \ 0.001) or lowered WISP-2 (p \ 0.001) expression was closely associated with decreased overall survival in both SC/ASC and AC patients. Multivariate Cox regression analysis showed that positive FGFBP1 expression (p = 0.001) or negative WISP-2 expression (p = 0.035 for SC/ASC and p = 0.009 for AC) is an independent predictor of poor prognosis in both SC/ASC and AC patients. We also revealed that differentiation, tumor size, TNM stage, lymph node metastasis, invasion, and surgical procedure were associated with survival of both SC/ASC and AC patients. Conclusion Our study suggested that the overexpression of FGFBP1 or loss of WISP-2 expression is closely related to the metastasis, invasion and poor prognosis of gallbladder cancer.
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- 2013
8. Spatial distribution and vertical variation of arsenic in Guangdong soil profiles, China
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Hu Yg, Lizhong Zhu, Li Dq, Wu Zf, Fei Li, Zhu La, Yuan Hx, and Huan Zhang
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inorganic chemicals ,China ,Health, Toxicology and Mutagenesis ,chemistry.chemical_element ,Soil science ,Toxicology ,Arsenic ,Soil ,Soil retrogression and degradation ,Soil Pollutants ,Particle Size ,Humic Substances ,Hydrology ,geography ,geography.geographical_feature_category ,integumentary system ,Bedrock ,Soil organic matter ,General Medicine ,Pollution ,Soil contamination ,chemistry ,Soil water ,Erosion ,Environmental science ,Soil horizon ,Environmental Monitoring - Abstract
Total of 260 soil profiles were reported to investigate the arsenic spatial distribution and vertical variation in Guangdong province. The arsenic concentration followed an approximately lognormal distribution. The arsenic geometric mean concentration of 10.4 mg/kg is higher than that of China. An upper baseline concentration of 23.4 mg/kg was estimated for surface soils. The influence of soil properties on arsenic concentration was not important. Arsenic spatial distributions presented similar patterns that high arsenic concentration mainly located in limestone, and sandshale areas, indicating that soil arsenic distribution was dependent on bedrock properties than anthropogenic inputs. Moreover, from A- to C-horizon arsenic geometric mean concentrations had an increasing tendency of 10.4, 10.7 to 11.3 mg/kg. This vertical variation may be related to the lower soil organic matter and soil degradation and erosion. Consequently, the soil arsenic export into surface and groundwaters would reach 1040 t year −1 in the study area.
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- 2005
9. A TAYLOR SERIES BASED INPUT VARIABLE SELECTION FOR NONLINEAR SYSTEM
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Song GW(宋广为) and Li DQ(李德强)
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Mathematical optimization ,Nonlinear system ,Adaptive neuro fuzzy inference system ,symbols.namesake ,Taylor series ,symbols ,Applied mathematics ,Partial derivative ,Feature selection ,Linear equation ,Selection (genetic algorithm) ,Mathematics ,Variable (mathematics) - Abstract
Input variable selection is always a significant problem in nonlinear system modeling. In this paper, we propose an effective and efficient input variable selection method based on Taylor series. We assume the nonlinear system as an at least 3 order derivative and continuous function f(x), and approximate it with 3 order Taylor series in the small proximities of the sample data. We define the average absolute values of first order partial derivates at the sample data as the importance indexes for the input variable candidates, and the average absolute values of second order partial derivatives as the correlation degrees between the input candidates. The importance indexes of the input variable candidates can be calculated by solving the linear equation at low computational cost. In this paper, we describe the nonlinear system as an Adaptive Network Based Fuzzy Inference System (ANFIS). Guided by the priori knowledge of input variables, importance indexes and correlation degrees, a fast input variable selection method is presented in which Regularity Criterion is adopted to evaluate the performance of the selected input variables. Experiment results and theoretical analysis both prove that this input variable selection method can speed up the selection process and is more powerful than the typical search tree method.
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- 2005
10. The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)2SO4 aqueous two-phase system
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Jun Lu, Li Dq, and Xu Y
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Ammonium sulfate ,Aqueous solution ,Chemical Phenomena ,Chemistry, Physical ,Inorganic chemistry ,Static Electricity ,Aqueous two-phase system ,Serum Albumin, Bovine ,General Medicine ,Polyethylene glycol ,Hydrogen-Ion Concentration ,Biochemistry ,Polyethylene Glycols ,Partition coefficient ,chemistry.chemical_compound ,chemistry ,Ammonium Sulfate ,Potassium thiocyanate ,PEG ratio ,Muramidase ,Lysozyme ,Thiocyanates ,Biotechnology - Abstract
The effect of potassium thiocyanate on the partitioning of lysozyme and BSA in polyethylene glycol 2000/ammonium sulfate aqueous two-phase system has been investigated. As a result of the addition of potassium thiocyanate to the PEG/ammonium sulfate system, the PEG/mixed salts aqueous two-phase system was formed. It was found that the potassium thiocyanate could alter the pH difference between the two phases, and, thus, influence the partition coefficients of the differently charged proteins. The relationship between partition coefficient of the proteins and pH difference between two phases has been discussed. It was proposed that the pH difference between two phases could be employed as the measurement of electrostatic driving force for the partitioning of charged proteins in polyethylene glycol 2000/ammonium sulfate aqueous two-phase system.
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- 2001
11. Wuling Capsule promotes hippocampal neurogenesis by improving expression of connexin 43 in rats exposed to chronic unpredictable mild stress
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Li, DQ, primary
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- 2010
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12. BiClamp((R)) forceps was significantly superior to conventional suture ligation in radical abdominal hysterectomy: a retrospective cohort study in 391 cases.
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Li L, Qie MR, Wang XL, Huang J, Zhang Q, Li DQ, and He YD
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- 2012
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13. Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis.
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Li DQ, Hou YF, Wu J, Chen Y, Lu JS, Di GH, Ou ZL, Shen ZZ, Ding J, and Shao ZM
- Abstract
To study the molecular mechanisms underlying breast cancer metastasis, gene expression profile analysis was performed on two well-established breast cancer cell lines with high and low metastatic potentials: MDA-MB-435HM and MDA-MB-435LM. The analysis was conducted using cDNA microarrays containing 8000 genes. Of 60 differentially expressed genes, ALL1-fused gene from chromosome 1q (AF1Q), a putative oncogene not described previously in breast cancer, was identified and found to be over-expressed in MDA-MB-435HM cells compared with MDA-MB-435LM cells. The results indicate that AF1Q may play an important role in breast cancer metastasis. To test this hypothesis, we generated an AF1Q high-expression cell line by stable transfection of AF1Q cDNA into MDA-MB-435LM cells. Results showed that over-expression of AF1Q led to a marked increase in the invasive and metastatic potential of MDA-MB-435LM cells in vitro and in vivo, accompanied by the up-regulation of matrix metalloproteinase-2 (MMP-2), MMP-9, transcription factor Ets-1, and RhoC expression in both mRNA and protein levels. Consistent with this observation, reduced AF1Q expression in MDA-MB-435HM cells by small interfering RNA (siRNA) resulted in a significant decrease in the invasive potential of MDA-MB-435HM cells in vitro and in the protein expression of MMP-2, MMP-9, Ets-1, and RhoC, compared with either parental or non-silencing control cells. These data provide functional evidence that oncogene AF1Q may be a novel mediator of metastasis promotion in human breast cancer through regulation of the MMP pathway and RhoC expression. [ABSTRACT FROM AUTHOR]
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- 2006
14. An improved method of determining the zeta-potential and surface conductance
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Erickson, D., Li, Dq, and Carsten Werner
15. Insight into the Effect of Dual Active Cu0/Cu+ Sites in a Cu/ZnO-Al2O3 Catalyst on 5-Hydroxylmethylfurfural Hydrodeoxygenation
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Qian Wang, Dianqing Li, Junting Feng, Yufei He, Zhi Yu, Paolo Fornasiero, Wang, Q, Yu, Z, Feng, Jt, Fornasiero, P, He, Yf, and Li, Dq
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LDH ,General Chemical Engineering ,media_common.quotation_subject ,Cu-based catalyst ,Biomass ,dual active sites ,02 engineering and technology ,010402 general chemistry ,Furfural ,01 natural sciences ,Competition (biology) ,Catalysis ,C-O bond activation ,chemistry.chemical_compound ,Environmental Chemistry ,LDHs ,media_common ,Renewable Energy, Sustainability and the Environment ,Chemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Dual (category theory) ,biofuel ,Chemical engineering ,Biofuel ,dual active site ,0210 nano-technology ,Hydrodeoxygenation - Abstract
The production of biofuel 2,5-dimethylfuran (DMF) from an important biomass platform of 5-hydroxymethyl furfural (HMF) involves a complicated reaction pathway due to the competition of C=O hydrogenation and C-O hydrogenolysis; however, the dependence of formed intermediates and catalytic sites needs to be further identified. Herein, we constructed a highly efficient and selective Cu/ZnO-Al2O3 catalyst via reduction of a CuZnAl-layer double hydroxide (LDH) precursor, aimed for studying the effect of Cu-0/Cu+ species on forming different intermediates and their synergetic promotional effect on HMF hydrodeoxygenation. As expected, the dependence of formation of different intermediates on the Cu+/Cu-0 ratio was observed; notably, Cu/ZnOAl2O3 with higher Cu+/Cu-0 ratio finally gave 90.1% DMF selectivity at 100% HMF conversion, more selective than CuNi/(AlO3)-O-2 (59.6%) and Cu/MgO-Al2O3 (75.8%) references. By a combined study of in situ IR experiments, the observed correlation between the reaction intermediates and the Cu-0/Cu+ site suggests that Cu-0 tends to adsorb the C=O bond as well as hydrogen molecule, while the Cu+ site is preferable to adsorb and activate the C-O bond. With the synergy of dual active sites, the Cu/ZnO-(AlO3)-O-2 catalyst showed both high activity and selectivity to DMF. This work provides deep insight into the nature of active Cu species in multiple functional group biomass platform transformation.
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- 2020
16. Images in cardiology. Aberrant origin of circumflex coronary artery from left subclavian artery.
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Li CB, Bi YW, Sun WY, Li RJ, Li GS, You BA, Chen WQ, Li DQ, Chen YG, Zhang Y, Li, Chuan-Bao, Bi, Yan-Wen, Sun, Wen-Yu, Li, Rui-Jian, Li, Gui-Shuang, You, Bei-An, Chen, Wen-Qiang, Li, Da-Qing, Chen, Yu-Guo, and Zhang, Yun
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- 2011
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17. The DRAP1/DR1 Repressor Complex Increases mTOR Activity to Promote Progression and Confer Everolimus Sensitivity in Triple-Negative Breast Cancer.
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Huang MY, Hu SY, Dong J, Deng L, Andriani L, Ma XY, Zhang YL, Zhang FL, Shao ZM, and Li DQ
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- Humans, Female, Animals, Mice, Cell Proliferation drug effects, Cell Line, Tumor, Gene Expression Regulation, Neoplastic drug effects, Xenograft Model Antitumor Assays, Mice, Nude, Cell Movement drug effects, Mice, Inbred BALB C, Triple Negative Breast Neoplasms pathology, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms genetics, TOR Serine-Threonine Kinases metabolism, Everolimus pharmacology, Disease Progression
- Abstract
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. Transcriptional dysregulation is a hallmark of cancer, and several transcriptional regulators have been demonstrated to contribute to cancer progression. In this study, we identified an upregulation of the transcriptional corepressor downregulator of transcription 1-associated protein 1 (DRAP1) in TNBC, which was closely associated with poor recurrence-free survival in patients with TNBC. DRAP1 promoted TNBC proliferation, migration, and invasion in vitro and tumor growth and metastasis in vivo. Mechanistically, the downregulator of transcription 1 (DR1)/DRAP1 heterodimer complex inhibited expression of the cytosolic arginine sensor for mTORC1 subunit 1 (CASTOR1) and thereby increased activation of mTOR, which sensitized TNBC to treatment with the mTOR inhibitor everolimus. DRAP1 and DR1 also formed a positive feedback loop. DRAP1 enhanced the stability of DR1 by recruiting the deubiquitinase USP7 to inhibit its proteasomal degradation; in turn, DR1 directly promoted DRAP1 transcription. Collectively, this study uncovered a DRAP1-DR1 bidirectional regulatory pathway that promotes TNBC progression, suggesting that targeting the DRAP1/DR1 complex might be a potential therapeutic strategy to treat TNBC. Significance: DR1 and DRAP1 form a positive feedback loop and a repressor complex to cooperatively inhibit cytosolic arginine sensor for mTORC1 subunit 1 transcription and stimulate mTOR signaling, leading to progression and increased everolimus sensitivity in triple-negative breast cancer., (©2024 American Association for Cancer Research.)
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- 2024
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18. Sideroflexin-1 promotes progression and sensitivity to lapatinib in triple-negative breast cancer by inhibiting TOLLIP-mediated autophagic degradation of CIP2A.
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Andriani L, Ling YX, Yang SY, Zhao Q, Ma XY, Huang MY, Zhang YL, Zhang FL, Li DQ, and Shao ZM
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- Animals, Female, Humans, Mice, Antineoplastic Agents pharmacology, Cell Line, Tumor, Cell Proliferation drug effects, Disease Progression, Gene Expression Regulation, Neoplastic drug effects, Intracellular Signaling Peptides and Proteins metabolism, Intracellular Signaling Peptides and Proteins genetics, Proteolysis drug effects, Signal Transduction drug effects, Xenograft Model Antitumor Assays, Autoantigens metabolism, Autoantigens genetics, Autophagy drug effects, Lapatinib pharmacology, Membrane Proteins metabolism, Membrane Proteins genetics, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms pathology, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms genetics, Cation Transport Proteins genetics, Cation Transport Proteins metabolism
- Abstract
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and it lacks specific therapeutic targets and effective treatment protocols. By analyzing a proteomic TNBC dataset, we found significant upregulation of sideroflexin 1 (SFXN1) in tumor tissues. However, the precise function of SFXN1 in TNBC remains unclear. Immunoblotting was performed to determine SFXN1 expression levels. Label-free quantitative proteomics and liquid chromatography-tandem mass spectrometry were used to identify the downstream targets of SFXN1. Mechanistic studies of SFXN1 and cellular inhibitor of PP2A (CIP2A) were performed using immunoblotting, immunofluorescence staining, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Functional experiments were used to investigate the role of SFXN1 in TNBC cells. SFXN1 was significantly overexpressed in TNBC tumor tissues and was associated with unfavorable outcomes in patients with TNBC. Functional experiments demonstrated that SFXN1 promoted TNBC growth and metastasis in vitro and in vivo. Mechanistic studies revealed that SFXN1 promoted TNBC progression by inhibiting the autophagy receptor TOLLIP (toll interacting protein)-mediated autophagic degradation of CIP2A. The pro-tumorigenic effect of SFXN1 overexpression was partially prevented by lapatinib-mediated inhibition of the CIP2A/PP2A/p-AKT pathway. These findings may provide a new targeted therapy for patients with TNBC., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
19. Maleic anhydride-functionalized cellulose nanocrystal-stabilized high internal phase Pickering emulsion for pesticide delivery.
- Author
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Wu C, Li J, Ding JW, Jiang H, Su HF, and Li DQ
- Subjects
- Nitro Compounds chemistry, Temperature, Drug Liberation, Cellulose chemistry, Nanoparticles chemistry, Maleic Anhydrides chemistry, Emulsions chemistry, Pesticides chemistry, Neonicotinoids chemistry
- Abstract
The salt-responsiveness of Pickering emulsions has significantly influenced their applications due to the large amount of salt on the surface of plant leaves. The present study provided a maleic anhydride-functionalized cellulose nanocrystal-stabilized high internal phase Pickering emulsion (MACNCs-HIPPEs) that was stable to high-concentration salt and used for pesticide delivery. The stability of MACNCs-HIPPEs was investigated by adjusting the oil-phase volume fraction (φ), the MACNCs concentration, NaCl dosages, and the rheological properties. The high internal phase Pickering emulsion was obtained at φ of 0.8 and MACNCs concentration of 2wt% and showed excellent salt stability (NaCl, 1200 mM) and significant storage stability (60 days). The sustained release of imidacloprid (IMI) from imidacloprid-loaded MACNCs-HIPPEs (IMI@MACNCs-HIPPEs) showed a positive correlation to the temperature (15°C, 25°C, 35°C), indicating clear thermo-responsiveness of the prepared pesticide formulation. The test of spread and retention of IMI@MACNCs-HIPPEs on the leaf surface showed a significant advantage compared with the commercial IMI water dispersible granules (CG). All the advantages mentioned above showed the excellent potential of the MACNCs-HIPPEs in delivering lipophilic pesticides., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
20. Fabrication of thermo-responsive microcapsule pesticide delivery system from maleic anhydride-functionalized cellulose nanocrystals-stabilized pickering emulsion template.
- Author
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Li J, Wu C, Xu HJ, Ding JW, Li YJ, Jiang H, Su HF, Chen S, Li X, Javaid MA, and Li DQ
- Subjects
- Animals, Neonicotinoids chemistry, Drug Liberation, Temperature, Nitro Compounds chemistry, Mice, Drug Delivery Systems methods, Drug Carriers chemistry, Acrylamides, Maleic Anhydrides chemistry, Cellulose chemistry, Nanoparticles chemistry, Pesticides chemistry, Emulsions chemistry, Capsules chemistry
- Abstract
The overuse of pesticides has shown their malpractices. Novel and sustainable formulations have consequently attracted abundant attention but still appear to have drawbacks. Here, we use a maleic anhydride-functionalized cellulose nanocrystals-stabilized Pickering emulsions template to prepare thermo-responsive microcapsules for a pesticide delivery system via radical polymerization with N-isopropyl acrylamide. The microcapsules (MACNCs-g-NIPAM) are characterized by the microscope, SEM, FTIR, XRD, TG-DTG, and DSC techniques. Imidacloprid (IMI) is loaded on MACNCs-g-NIPAM to form smart release systems (IMI@MACNCs-g-NIPAM) with high encapsulation efficiency (~88.49%) and loading capability (~55.02%). The IMI@MACNCs-g-NIPAM present a significant thermo-responsiveness by comparing the release ratios at 35°C and 25°C (76.22% vs 50.78%). It also exhibits advantages in spreadability, retention and flush resistance on the leaf surface compared with the commercial IMI water-dispersible granules (CG). IMI@MACNCs-g-NIPAM also manifest a significant advantage over CG (11.12 mg/L vs 38.90 mg/L for LC
50 ) regarding activity tests of targeted organisms. In addition, IMI@MACNCs-g-NIPAM has shown excellent biocompatibility and low toxicity. All the benefits mentioned above prove the excellent potential of IMI@MACNCs-g-NIPAM as a smart pesticide formulation., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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21. A handy way for forming N-doped TiO 2 /carbon from pectin and d,l-serine hydrazide hydrochloride.
- Author
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Xiong ZW, Guo LN, Jiang H, Zhang Y, Gao Q, Li J, Chen S, Li X, Javaid MA, Li DQ, and Xie H
- Subjects
- Nitrogen chemistry, Catalysis, Photolysis, Porosity, Methylene Blue chemistry, Pectins chemistry, Titanium chemistry, Carbon chemistry, Serine chemistry
- Abstract
N-doped TiO
2 /carbon composites (N-TiPC) have shown excellent photodegradation performances to the organic contaminants but are limited by the multistage preparation (i.e., preparation of porous carbon, preparation of N-doped TiO2 , and loading of N-doped TiO2 on porous carbon). Here, we develop a handy way by combining the Pickering emulsion-gel template route and chelation reaction of polysaccharides. The N-TiPC is obtained by calcinating pectin/Dl-serine hydrazide hydrochloride (SHH)-Ti4+ chelate and is further described by modern characterization techniques. The results show that the N atom is successfully doped into the TiO2 lattice, and the bandgap value of N-TiPC is reduced to 2.3 eV. Moreover, the particle size of N-TiPC remains about 10 nm. The configurations of the composites are simulated using DFT calculation. The photocatalytic experiments show that N-TiPC has a high removal efficiency for methylene blue (MB) and oxytetracycline hydrochloride (OTC-HCL). The removal ratios of MB (20 mg/L, 50 mL) and OTC-HCL (30 mg/L, 50 mL) are 99.41 % and 78.29 %, respectively. The cyclic experiments show that the photocatalyst has good stability. Overall, this study provides a handy way to form N-TiPC with enhanced photodegradation performances. It can also be promoted to other macromolecules such as cellulose and its derivatives, sodium alginate, chitosan, lignin, etc., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
22. The mysteries of pharmacokinetics and in vivo metabolism of Oroxylum indicum (L.) Kurz: A new perspective from MSOP method.
- Author
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Zhang X, Zhang Y, Wang N, Liu J, Zhang LT, Zhang ZQ, and Li DQ
- Abstract
The pharmacological effects of flavonoids in Oroxylum indicum (L.) Kurz against inflammation, bacterial, and oxidation have been well-documented. Additionally, it is commonly consumed as tea. However, the in vivo mechanism of its main compounds has not been well elucidated. In this study, a highly selective and sensitive UHPLC-Q-TOF-MS method combined with Mass Spectrum-based Orthogonal Projection (MSOP) theory and four-step analytical strategy was established and validated to identify metabolites in rats following oral administration Oroxylum indicum (L.) Kurz extract. Furthermore, a sensitive LC-MS/MS method was developed and validated for the first time to analyze the pharmacokinetics of ten main flavonoids in rats. Notably, a total of 47 metabolites were identified in blood, bile, urine, and feces samples. The maximum plasma concentration (C
max ) values for oroxin A, oroxin B, baicalin, chrysin, baicalein, scutellarein, apigenin, quercetin oroxylin A and isorhamnetin were 2945.1 ± 11.23 ng/mL, 3123.9 ± 16.37 ng/mL, 130.40 ± 27.52 ng/mL, 117.20 ± 28.54 ng/mL, 64.12 ± 19.33 ng/mL, 97.22 ± 24.27 ng/mL, 145.22 ± 29.92 ng/mL, 45.19 ± 18.84 ng/mL, 67.32 ± 15.78 ng/mL and 128.44 ± 26.42 ng/mL. A double peak was observed in the drug-time curve of apigenin, due to enterohepatic recirculation. This study demonstrated that MSOP method provided more technical support for the identification of flavonoid metabolites in complex system than traditional methods., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)- Published
- 2024
- Full Text
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23. Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model.
- Author
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Lin N, Chen X, Liu H, Gao N, Liu Z, Li J, Pflugfelder SC, and Li DQ
- Subjects
- Animals, Mice, Conjunctiva metabolism, Conjunctiva drug effects, Conjunctiva pathology, Enzyme-Linked Immunosorbent Assay, Female, Epithelium, Corneal metabolism, Epithelium, Corneal drug effects, Real-Time Polymerase Chain Reaction, RNA, Messenger genetics, RNA, Messenger metabolism, Amino Acids, Diamino, Dry Eye Syndromes metabolism, Dry Eye Syndromes drug therapy, Mice, Inbred C57BL, Disease Models, Animal, Goblet Cells metabolism, Goblet Cells drug effects, Goblet Cells pathology, Interferon-gamma metabolism, Mucins metabolism, Mucins biosynthesis, Mucins genetics, Interleukin-13 metabolism
- Abstract
Purpose: This study aimed to explore protective effects and potential mechanism of ectoine, a natural osmoprotectant, on ocular surface mucin production in dry eye disease., Methods: A dry eye model was established in C57BL/6 mice exposed to desiccating stress (DS) with untreated (UT) mice as controls. DS mice were topically treated with 2.0% ectoine or PBS vehicle. Corneal epithelial defects were assessed by Oregon Green Dextran (OGD) fluorescent staining. Conjunctival goblet cells, ocular mucins, and T help (Th) cytokines were evaluated by immunofluorescent staining or ELISA, and RT-qPCR., Results: Compared with UT mice, corneal epithelial defects were detected as strong punctate OGD fluorescent staining in DS mice with vehicle, whereas ectoine treatment largely reduced OGD staining to near-normal levels. Conjunctival goblet cell density and cell size decreased markedly in DS mice, but was significantly recovered by ectoine treatment. The protein production and mRNA expression of two gel-forming secreted MUC5AC and MUC2, and 4 transmembrane mucins, MUC1, MUC4, MUC16, and MUC15, largely decreased in DS mice, but was restored by ectoine. Furthermore, Th2 cytokine IL-13 was inhibited, whereas Th1 cytokine IFN-γ was stimulated at protein and mRNA levels in conjunctiva and draining cervical lymph nodes (CLNs) of DS mice, leading to decreased IL-13/IFN-γ ratio. Interestingly, 2.0% ectoine reversed their alternations and restored IL-13/IFN-γ balance., Conclusions: Our findings demonstrate that topical ectoine significantly reduces corneal damage, and enhances goblet cell density and mucin production through restoring imbalanced IL-13/IFN-γ signaling in murine dry eye model. This suggests therapeutic potential of natural osmoprotectant ectoine for dry eye disease.
- Published
- 2024
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- View/download PDF
24. [Relationship between DTA Mutations and Thromboembolism in Patients with Myeloproliferative Neoplasms].
- Author
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Wang M, Zhao HY, Li DQ, and Chen P
- Subjects
- Humans, Middle Aged, Retrospective Studies, DNA (Cytosine-5-)-Methyltransferases genetics, Male, Female, High-Throughput Nucleotide Sequencing, Mutation, Dioxygenases, Myeloproliferative Disorders genetics, Myeloproliferative Disorders complications, Thromboembolism genetics, Proto-Oncogene Proteins genetics, DNA-Binding Proteins genetics, Repressor Proteins genetics, DNA Methyltransferase 3A
- Abstract
Objective: To analyze the DTA ( DNMT3A , TET2 , ASXL1 ) mutations in patients with myeloproliferative neoplasms (MPN), and preliminarily explore their correlation with thromboembolism., Methods: Clinical characteristics of 62 patients diagnosed de novo MPN at Central Hospital Affiliated to Shandong First Medical University from September 2016 to September 2022 were retrospectively analyzed. Next-generation sequencing was used to detect 35 MPN-related genes, and the DTA mutations in MPN patients and their relationship with thromboembolic events were analyzed., Results: 75.8% (47/62) of the patients presented pathogenic non-driver mutations, and the mean number of pathogenic non-driver mutations per patient was 1.08. Among them, the most frequently mutated non-driver genes were TET2 (38.7%, 24/62), DNMT3A (9.7%, 6/62) and ASXL1 (6.5%, 4/62). The presence of DTA gene mutations was 50% (31/62) in the total MPN patients, and mainly accompanied by driver mutations. The mutation rate of DTA in patients aged ≥60 years was significantly higher than that in patients <60 years old ( P =0.039). The incidence of thromboembolism in patients with DTA mutation was 58.1% (18/31), which was significantly higher than that in patients without DTA mutation (19.4%, 6/31) ( P =0.002). The TET2 gene mutation rate in MPN patients with thromboembolism was 66.7% (16/24), which was significantly higher than that in patients without thromboembolism (21.1%, 8/38) ( P =0.00)., Conclusion: Patients with MPN have a higher incidence of DTA mutations, which are mainly accompanied by driver gene mutations. The incidence of thromboembolism in MPN patients with DTA mutations is higher than that in patients without DTA mutations. Especially, the elderly (≥60 years) essential thrombocythemia(ET) and polycythemia vera(PV) patients with TET2 mutation should be vigilant for thromboembolic events.
- Published
- 2024
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25. SF3A2 promotes progression and cisplatin resistance in triple-negative breast cancer via alternative splicing of MKRN1.
- Author
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Deng L, Liao L, Zhang YL, Yang SY, Hu SY, Andriani L, Ling YX, Ma XY, Zhang FL, Shao ZM, and Li DQ
- Subjects
- Humans, Alternative Splicing, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, RNA Splicing Factors genetics, RNA Splicing Factors metabolism, Cisplatin pharmacology, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms genetics, Triple Negative Breast Neoplasms metabolism
- Abstract
Triple-negative breast cancer (TNBC) is the deadliest subtype of breast cancer owing to the lack of effective therapeutic targets. Splicing factor 3a subunit 2 (SF3A2), a poorly defined splicing factor, was notably elevated in TNBC tissues and promoted TNBC progression, as confirmed by cell proliferation, colony formation, transwell migration, and invasion assays. Mechanistic investigations revealed that E3 ubiquitin-protein ligase UBR5 promoted the ubiquitination-dependent degradation of SF3A2, which in turn regulated UBR5, thus forming a feedback loop to balance these two oncoproteins. Moreover, SF3A2 accelerated TNBC progression by, at least in part, specifically regulating the alternative splicing of makorin ring finger protein 1 ( MKRN1 ) and promoting the expression of the dominant and oncogenic isoform, MKRN1-T1 . Furthermore, SF3A2 participated in the regulation of both extrinsic and intrinsic apoptosis, leading to cisplatin resistance in TNBC cells. Collectively, these findings reveal a previously unknown role of SF3A2 in TNBC progression and cisplatin resistance, highlighting SF3A2 as a potential therapeutic target for patients with TNBC.
- Published
- 2024
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26. Cemiplimab for metastatic squamous cell carcinoma of the orbit, periocular adnexa, and thigh.
- Author
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Dhillon J, Li DQ, Burnier M, Shenouda G, Arthurs BP, and El-Hadad C
- Subjects
- Male, Humans, Middle Aged, Orbit pathology, Thigh pathology, Positron Emission Tomography Computed Tomography, Carcinoma, Squamous Cell pathology, Skin Neoplasms pathology, Orbital Neoplasms diagnostic imaging, Orbital Neoplasms drug therapy, Antibodies, Monoclonal, Humanized
- Abstract
The current case presentation highlights the potential of cemiplimab, a programmed cell death protein-1 (PD-1) inhibitor, as first-line treatment for periocular metastatic cutaneous squamous cell carcinoma (SCC) without requiring curative surgery or radiotherapy. A 64-year-old male presented with a progressing 4.5 × 3.0 cm left upper eyelid lesion initially diagnosed as psoriasis. Work-up revealed cutaneous SCC with tumor invasion into extraconal fat and lacrimal gland, and metastasis to the left parotid lymph node. The patient also presented with a suspicious lesion on his left medial thigh found to be a second primary on pathology. To avoid orbital exenteration and treat the multifocal disease, the patient was started on intravenous cemiplimab immunotherapy. Following six doses, repeated FGD-PET-CT revealed a complete response of the left eyelid lesion and residual low-grade hypermetabolic activity of the left medial thigh lesion. Biopsy confirmed chronic inflammation and fibrosis with no signs of malignancy. This unique case with dual primary cutaneous SCC provides support for cemiplimab in treating locally invasive periocular SCC, and potentially abrogating the need for highly morbid exenteration procedures to preserve binocular vision.
- Published
- 2024
- Full Text
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27. Aldehyde group pendant-grafted pectin-based injectable hydrogel.
- Author
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Li DQ, Tohti M, Fu YS, Zhang Y, Xiong ZW, Li J, and Guo YF
- Subjects
- Aldehydes, Spectroscopy, Fourier Transform Infrared, Polysaccharides chemistry, Hydrogels chemistry, Pectins
- Abstract
Periodate oxidation has been the widely accepted route for obtaining aldehyde group-functionalized polysaccharides but significantly influenced the various physicochemical properties due to the ring opening of the backbone of polysaccharides. The present study, for the first time, presents a novel method for the preparation of aldehyde group-functionalized polysaccharides that could retain the ring structure and the consequent rigidity of the backbone. Pectin was collected as the representative of polysaccharides and modified with cyclopropyl formaldehyde to obtain pectin aldehyde (AP), which was further crosslinked by DL-lysine (LYS) via the Schiff base reaction to prepare injectable hydrogel. The feasibility of the functionalization was proved by FT-IR and
1 H NMR techniques. The obtained hydrogel showed acceptable mechanical properties, self-healing ability, syringeability, and sustained-release performance. Also, as-prepared injectable hydrogel presented great biocompatibility with a cell proliferation rate of 96 %, and the drug-loaded hydrogel exhibited clear inhibition of cancer cell proliferation. Overall, the present study showed a new method for the preparation of aldehyde group-functionalized polysaccharides, and the drug-loaded hydrogel has potential in drug release applications., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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- View/download PDF
28. Ectoine protects corneal epithelial survival and barrier from hyperosmotic stress by promoting anti-inflammatory cytokine IL-37.
- Author
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Li JM, Lin N, Zhang Y, Chen X, Liu Z, Lu R, Bian F, Liu H, Pflugfelder SC, and Li DQ
- Subjects
- Humans, Cells, Cultured, Interleukin-1 metabolism, Interleukin-1 pharmacology, Enzyme-Linked Immunosorbent Assay, Microscopy, Confocal, Cell Proliferation drug effects, Cytokines metabolism, Cell Survival drug effects, Epithelium, Corneal metabolism, Epithelium, Corneal drug effects, Epithelium, Corneal pathology, Osmotic Pressure, Amino Acids, Diamino pharmacology
- Abstract
Purpose: To explore novel role and molecular mechanism of a natural osmoprotectant ectoine in protecting corneal epithelial cell survival and barrier from hyperosmotic stress., Methods: Primary human corneal epithelial cells (HCECs) were established from donor limbus. The confluent cultures in isosmolar medium were switched to hyperosmotic media (400-500 mOsM), with or without ectoine or rhIL-37 for different time periods. Cell viability and proliferation were evaluated by MTT or WST assay. The integrity of barrier proteins and the expression of cytokines and cathepsin S were evaluated by RT-qPCR, ELISA, and immunostaining with confocal microscopy., Results: HCECs survived well in 450mOsM but partially damaged in 500mOsM medium. Ectoine well protected HCEC survival and proliferation at 500mOsM. The integrity of epithelial barrier was significantly disrupted in HCECs exposed to 450mOsM, as shown by 2D and 3D confocal immunofluorescent images of tight junction proteins ZO-1 and occludin. Ectoine at 5-20 mM well protected these barrier proteins under hyperosmotic stress. The expression of TNF-α, IL-1β, IL-6 and IL-8 were dramatically stimulated by hyperosmolarity but significantly suppressed by Ectoine at 5-40 mM. Cathepsin S, which was stimulated by hyperosmolarity, directly disrupted epithelial barrier. Interestingly, anti-inflammatory cytokine IL-37 was suppressed by hyperosmolarity, but restored by ectoine at mRNA and protein levels. Furthermore, rhIL-37 suppressed cathepsin S and rescued cell survival and barrier in HCECs exposed to hyperosmolarity., Conclusion: Our findings demonstrate that ectoine protects HCEC survival and barrier from hyperosmotic stress by promoting IL-37. This provides new insight into pathogenesis and therapeutic potential for dry eye disease., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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29. Spermatid perinuclear RNA-binding protein promotes UBR5-mediated proteolysis of Dicer to accelerate triple-negative breast cancer progression.
- Author
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Chen SY, Zhang FL, Zhang YL, Liao L, Deng L, Shao ZM, Liu GY, and Li DQ
- Subjects
- Animals, Humans, Male, Mice, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Gene Expression Regulation, Neoplastic, Proteolysis, Semen metabolism, Spermatids metabolism, Spermatids pathology, Ubiquitin-Protein Ligases genetics, Ubiquitin-Protein Ligases metabolism, MicroRNAs genetics, MicroRNAs metabolism, Triple Negative Breast Neoplasms pathology
- Abstract
Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer with no targeted therapy. Spermatid perinuclear RNA binding protein (STRBP), a poorly characterized RNA-binding protein (RBP), has an essential role in normal spermatogenesis and sperm function, but whether and how its dysregulation contributing to cancer progression has not yet been explored. Here, we report that STRBP functions as a novel oncogene to drive TNBC progression. STRBP expression was upregulated in TNBC tissues and correlated with poor disease prognosis. Functionally, STRBP promoted TNBC cell proliferation, migration, and invasion in vitro, and enhanced xenograft tumor growth and lung colonization in mice. Mechanistically, STRBP interacted with Dicer, a core component of the microRNA biogenesis machinery, and promoted its proteasomal degradation through enhancing its interaction with E3 ubiquitin ligase UBR5. MicroRNA-sequencing analysis identified miR-200a-3p as a downstream effector of STRBP, which was regulated by Dicer and affected epithelial-mesenchymal transition. Importantly, the impaired malignant phenotypes of TNBC cells caused by STRBP depletion were largely rescued by knockdown of Dicer, and these effects were compromised by transfection of miR-200a-3p mimics. Collectively, these findings revealed a previously unrecognized oncogenic role of STRBP in TNBC progression and identified STRBP as a promising target against TNBC., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
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30. Polymerization strategy for cellulose nanocrystals-based photonic crystal films with water resisting property.
- Author
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Zhang Y, Li DQ, Yang CX, Xiong ZW, Tohti M, Zhang YQ, Chen HJ, and Li J
- Subjects
- Polymerization, Cellulose chemistry, Spectroscopy, Fourier Transform Infrared, Water chemistry, Nanoparticles chemistry
- Abstract
Cellulose nanocrystals (CNCs) can form a liquid crystal film with a chiral nematic structure by evaporative-induced self-assembly (EISA). It has attracted much attention as a new class of photonic liquid crystal material because of its intrinsic, unique structural characteristics, and excellent optical properties. However, the CNCs-based photonic crystal films are generally prepared via the physical crosslinking strategy, which present water sensitivity. Here, we developed CNCs-g-PAM photonic crystal film by combining free radical polymerization and EISA. FT-IR, SEM, POM, XRD, TG-DTG, and UV-Vis techniques were employed to characterize the physicochemical properties and microstructure of the as-prepared films. The CNCs-g-PAM films showed a better thermo-stability than CNCs-based film. Also, the mechanical properties were significantly improved, viz., the elongation at break was 9.4 %, and tensile strength reached 18.5 Mpa, which was a much better enhancement than CNCs-based film. More importantly, the CNCs-g-PAM films can resist water dissolution for more than 24 h, which was impossible for the CNCs-based film. The present study provided a promising strategy to prepare CNCs-based photonic crystal film with high flexibility, water resistance, and optical properties for applications such as decoration, light management, and anti-counterfeiting., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
31. Ectoine, from a Natural Bacteria Protectant to a New Treatment of Dry Eye Disease.
- Author
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Chen X, Lin N, Li JM, Liu H, Abu-Romman A, Yaman E, Bian F, de Paiva CS, Pflugfelder SC, and Li DQ
- Abstract
Ectoine, a novel natural osmoprotectant, protects bacteria living in extreme environments. This study aimed to explore the therapeutic effect of ectoine for dry eye disease. An experimental dry eye model was created in C57BL/6 mice exposed to desiccating stress (DS) with untreated mice as controls (UT). DS mice were dosed topically with 0.5-2.0% of ectoine or a vehicle control. Corneal epithelial defects were detected via corneal smoothness and Oregon Green dextran (OGD) fluorescent staining. Pro-inflammatory cytokines and chemokines were evaluated using RT-qPCR and immunofluorescent staining. Compared with UT mice, corneal epithelial defects were observed as corneal smoothness irregularities and strong punctate OGD fluorescent staining in DS mice with vehicle. Ectoine treatment protected DS mice from corneal damage in a concentration-dependent manner, and ectoine at 1.0 and 2.0% significantly restored the corneal smoothness and reduced OGD staining to near normal levels. Expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and chemokines CCL3 and CXCL11 was significantly elevated in the corneas and conjunctivas of DS mice, whereas 1.0 and 2.0% ectoine suppressed these inflammatory mediators to near normal levels. Our findings demonstrate that ectoine can significantly reduce the hallmark pathologies associated with dry eye and may be a promising candidate for treating human disease.
- Published
- 2024
- Full Text
- View/download PDF
32. Simhypo-sand: a simple hypoplastic model for granular materials and SPH implementation.
- Author
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Wang S, Fang HJ, Kang X, Li DQ, and Wu W
- Abstract
This paper introduces a new hypoplastic model characterized by a simple and elegant formulation. It requires only 7 material parameters to depict salient mechanical behaviors of granular materials. The numerical implementation employs an explicit integration method, enhanced by a best-fit stress correction algorithm in a smoothed particle hydrodynamics code. The performance of this model in capturing soil behavior across a range of scenarios is demonstrated by conducting various numerical tests, including triaxial and simple shear at low strain rates, as well as granular collapse, rigid penetration and landslide process at high strain rates., Competing Interests: Conflict of interestThe authors declare no competing interests, (© The Author(s) 2024.)
- Published
- 2024
- Full Text
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33. Cellulose Dissolution, Modification, and the Derived Hydrogel: A Review.
- Author
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Wu C, Li J, Zhang YQ, Li X, Wang SY, and Li DQ
- Abstract
The cellulose-based hydrogel has occupied a pivotal position in almost all walks of life. However, the native cellulose can not be directly used for preparing hydrogel due to the complex non-covalent interactions. Some literature has discussed the dissolution and modification of cellulose but has yet to address the influence of the pretreatment on the as-prepared hydrogels. Firstly, the "touching" of cellulose by derived and non-derived solvents was introduced, namely, the dissolution of cellulose. Secondly, the "conversion" of functional groups on the cellulose surface by special routes, which is the modification of cellulose. The above-mentioned two parts were intended to explain the changes in physicochemical properties of cellulose by these routes and their influences on the subsequent hydrogel preparation. Finally, the "reinforcement" of cellulose-based hydrogels by physical and chemical techniques was summarized, viz., improving the mechanical properties of cellulose-based hydrogels and the changes in the multi-level structure of the interior of cellulose-based hydrogels., (© 2023 Wiley-VCH GmbH.)
- Published
- 2023
- Full Text
- View/download PDF
34. C9orf142 transcriptionally activates MTBP to drive progression and resistance to CDK4/6 inhibitor in triple-negative breast cancer.
- Author
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Liao L, Deng L, Zhang YL, Yang SY, Andriani L, Hu SY, Zhang FL, Shao ZM, and Li DQ
- Subjects
- Humans, Animals, Mice, Cell Line, Tumor, Cell Proliferation genetics, Up-Regulation genetics, Carrier Proteins genetics, Cyclin-Dependent Kinase 4 genetics, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms genetics, Triple Negative Breast Neoplasms pathology
- Abstract
Background: Triple-negative breast cancer (TNBC) presents the most challenging subtype of all breast cancers because of its aggressive clinical phenotypes and absence of viable therapy targets. In order to identify effective molecular targets for treating patients with TNBC, we conducted an integration analysis of our recently published TNBC dataset of quantitative proteomics and RNA-Sequencing, and found the abnormal upregulation of chromosome 9 open reading frame 142 (C9orf142) in TNBC. However, the functional roles of C9orf142 in TNBC are unclear., Methods: In vitro and in vivo functional experiments were performed to assess potential roles of C9orf142 in TNBC. Immunoblotting, real-time quantitative polymerase chain reaction (RT-qPCR), and immunofluorescent staining were used to investigate the expression levels of C9orf142 and its downstream molecules. The molecular mechanisms underlying C9orf142-regulated mouse double minute 2 (MDM2)-binding protein (MTBP) were determined by chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays., Results: In TNBC tissues and metastatic lymph nodes, we observed that C9orf142 exhibited abnormal up-regulation, and its elevated expression was indicative of unfavorable prognosis for TNBC patients. Both in vitro and in vivo functional experiments demonstrated that C9orf142 accelerated TNBC growth and metastasis. Further mechanism exploration revealed that C9orf142 transcriptionally activated MTBP, thereby regulating its downstream MDM2/p53/p21 signaling axis and the transition of cell cycle from G1 to S phase. Functional rescue experiment demonstrated that knockdown of MTBP attenuated C9orf142-mediated tumour growth and metastasis. Furthermore, depletion of C9orf142 remarkably increased the responsiveness of TNBC cells to CDK4/6 inhibitor abemaciclib., Conclusions: Together, these findings unveil a previously unrecognized effect of C9orf142 in TNBC progression and responsiveness to CDK4/6 inhibitor, and emphasize C9orf142 as a promising intervention target for TNBC treatment., (© 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.)
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- 2023
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35. Acylhydrazone-derived whole pectin-based hydrogel as an injectable drug delivery system.
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Wang SY, Tohti M, Zhang JQ, Li J, and Li DQ
- Subjects
- Humans, Hydrogen-Ion Concentration, MCF-7 Cells, Mice, Drug Carriers chemistry, Animals, Injections, Pectins chemistry, Hydrazones chemistry, Hydrogels chemistry, Drug Delivery Systems, Drug Liberation
- Abstract
Injectable hydrogel-based drug delivery systems have attracted more and more attention due to their sustained-release performance, biocompatibility, and 3D network. The present study showed whole pectin-based hydrogel as an injectable drug delivery system, which was developed from oxidized pectin (OP) and diacylhydrazine adipate-functionalized pectin (Pec-ADH) via acylhydrazone linkage. The as-prepared hydrogels were characterized by
1 H NMR, FT-IR, and SEM techniques. The equilibrium swelling ratio of obtained hydrogel (i.e., sample gel 5) was up to 4306.65 % in the distilled water, which was higher than that in PBS with different pH values. Increasing the pH of the swelling media, the swelling ratio of all hydrogels decreased significantly. The results that involved the swelling properties indicated the salt- and pH-responsiveness of the as-prepared hydrogels. The drug release study presented that 5-FU can be persistently released for more than 12 h without sudden release. Moreover, the whole pectin-based hydrogel presented high cytocompatibility toward L929 cell lines, and the drug delivery system showed a high inhibitory effect on MCF-7 cell lines. All these results manifested that the acylhydrazone-derived whole pectin-based hydrogel was an excellent candidate for injectable drug delivery systems., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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36. ETHE1 Accelerates Triple-Negative Breast Cancer Metastasis by Activating GCN2/eIF2α/ATF4 Signaling.
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Yang SY, Liao L, Hu SY, Deng L, Andriani L, Zhang TM, Zhang YL, Ma XY, Zhang FL, Liu YY, and Li DQ
- Subjects
- Humans, Eukaryotic Initiation Factor-2 metabolism, Cell Line, Tumor, Signal Transduction, Cell Proliferation genetics, Cell Movement genetics, Gene Expression Regulation, Neoplastic, Mitochondrial Proteins metabolism, Nucleocytoplasmic Transport Proteins metabolism, Activating Transcription Factor 4 genetics, Activating Transcription Factor 4 metabolism, Triple Negative Breast Neoplasms pathology
- Abstract
Triple-negative breast cancer (TNBC) is the most fatal subtype of breast cancer; however, effective treatment strategies for TNBC are lacking. Therefore, it is important to explore the mechanism of TNBC metastasis and identify its therapeutic targets. Dysregulation of ETHE1 leads to ethylmalonic encephalopathy in humans; however, the role of ETHE1 in TNBC remains elusive. Stable cell lines with ETHE1 overexpression or knockdown were constructed to explore the biological functions of ETHE1 during TNBC progression in vitro and in vivo. Mass spectrometry was used to analyze the molecular mechanism through which ETHE1 functions in TNBC progression. ETHE1 had no impact on TNBC cell proliferation and xenograft tumor growth but promoted TNBC cell migration and invasion in vitro and lung metastasis in vivo. The effect of ETHE1 on TNBC cell migratory potential was independent of its enzymatic activity. Mechanistic investigations revealed that ETHE1 interacted with eIF2α and enhanced its phosphorylation by promoting the interaction between eIF2α and GCN2. Phosphorylated eIF2α in turn upregulated the expression of ATF4, a transcriptional activator of genes involved in cell migration and tumor metastasis. Notably, inhibition of eIF2α phosphorylation through ISRIB or ATF4 knockdown partially abolished the tumor-promoting effect of ETHE1 overexpression. ETHE1 has a functional and mechanistic role in TNBC metastasis and offers a new therapeutic strategy for targeting ETHE1-propelled TNBC using ISRIB.
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- 2023
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37. Timing of gestational diabetes diagnosis, gestational weight gains and offspring growth trajectory: a prospective birth cohort study.
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Yin XG, Wang P, Zhou MT, Li DQ, Tao RX, Tao FB, Wang Y, and Zhu P
- Subjects
- Child, Preschool, Child, Infant, Pregnancy, Humans, Female, Cohort Studies, Prospective Studies, Weight Gain, Gestational Weight Gain, Diabetes, Gestational diagnosis
- Abstract
Background: The evidence on the associations of the timing of maternal gestational diabetes mellitus (GDM) with the comprehensive growth trajectory from perinatal to early childhood in offspring is limited. The potential mechanism remains elusive. Our aim is to estimate the associations of the timing of GDM diagnosis and gestational weight gains (GWG) with the growth trajectory of children from perinatal to early childhood., Methods: A total of 7609 participants are included from the Maternal & Infants Health in Hefei cohort study. Primary predictors were the timing of maternal GDM diagnosis and GWG during pregnancy. The main outcomes included fetal ultrasonic measurements, birth size as well as BMI peak indicators during infancy within 48 months., Results: GDM diagnosed before 26 weeks was associated with increased risks of overgrowth for fetal abdominal circumference (OR 1.19, 95% CI 1.04-1.36) and birth weight (OR 1.51, 95% CI 1.19-1.91) when compared with unexposed. GDM diagnosis < 26 weeks was related to the higher BMI peak (β 0.16, 95%CI 0.03-0.28) within 48 months. The significantly additive impacts of maternal early GDM diagnosis and excessive gestational weight gains (EGWG) on offspring overgrowth were observed. Women in GDM < 26 weeks with early EGWG group had higher levels of hsCRP compared with GDM > 26 weeks (P < 0.001)., Conclusions: Exposure to maternal GDM diagnosed before 26 weeks with early EGWG could lead to shifts and/or disruptions from the typical growth trajectory from perinatal to early childhood in offspring., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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38. Ti 4+ -dopamine/sodium alginate multicomponent complex derived N-doped TiO 2 @carbon nanocomposites for efficient removal of methylene blue.
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Xiong ZW, Meng YJ, Luo CB, Liu ZQ, Li DQ, and Li J
- Subjects
- Methylene Blue, Titanium, Dopamine, Alginates, Spectroscopy, Fourier Transform Infrared, Catalysis, Carbon, Nanocomposites
- Abstract
A one-pot route for the preparation of TiO
2 @carbon nanocomposite from Ti4+ /polysaccharide coordination complex has been developed and shown advantages in operation, cost, environment, etc. However, the photodegradation rate of methylene blue (MB) needs to be improved. N-doping has been proven as an efficient means to enhance photodegradation performance. Thus, the present study upgraded the TiO2 @carbon nanocomposite to N-doped TiO2 @carbon nanocomposite (N-TiO2 @C) from Ti4+ -dopamine/sodium alginate multicomponent complex. The composites were characterized by FT-IR, XRD, XPS, UV-vis DRS, TG-DTA, and SEM-EDS. The obtained TiO2 was a typical rutile phase, and the carboxyl groups existed on N-TiO2 @C. The photocatalyst consequently showed high removal efficiency of MB. The cycling experiment additionally indicated the high stability of N-TiO2 @C. The present work provided a novel route for preparing N-TiO2 @C. Moreover, it can be extended to prepare N-doped polyvalent metal oxides@carbon composites from all water-soluble polysaccharides such as cellulose derivatives, starch, and guar gum., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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39. Modulation of gut microbiota alleviates cerebral ischemia/reperfusion injury in rats by inhibiting M1 polarization of microglia.
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Li HJ, Li DQ, Zhang YL, Ding XF, Gao HT, Zhu Y, Liu J, Zhang LX, Chen J, Chen G, and Yu Y
- Abstract
Gut microbiota affects the gut-brain axis; hence, the modulation of the microbiota has been proposed as a potential therapeutic strategy for cerebral ischemia/reperfusion injury (CIRI). However, the role and mechanism of the gut microbiota in regulating microglial polarization during CIRI remain poorly understood. Herein, using a middle cerebral artery occlusion and reperfusion (MCAO/R) rat model, we evaluated changes in the gut microbiota after CIRI and the potential effects of fecal microbiota transplant (FMT) on the brain. Rats underwent either MCAO/R or sham surgery, and then they received FMT (started 3 days later; continued for 10 days). 2,3,5-Triphenyltetrazolium chloride staining, neurological outcome scale, and Fluoro-Jade C staining showed that MCAO/R induced cerebral infarction, neurological deficits, and neuronal degeneration. In addition, immunohistochemistry or real-time PCR assay showed increased expression levels of M1-macrophage markers-TNF-α, IL-1β, IL-6, and iNOS-in the rats following MCAO/R. Our finding suggests that microglial M1 polarization is involved in CIRI. 16 S ribosomal RNA gene sequencing data revealed an imbalance in the gut microbiota of MCAO/R animals. In contrast, FMT reversed this MCAO/R-induced imbalance in the gut microbiota and ameliorated nerve injury. In addition, FMT prevented the upregulation in the ERK and NF-κB pathways, which reversed the M2-to-M1 microglial shift 10 days after MCAO/R injury in rats. Our primary data showed that the modulation of the gut microbiota can attenuate CIRI in rats by inhibiting microglial M1 polarization through the ERK and NF-κB pathways. However, an understanding of the underlying mechanism requires further study., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Li, Li, Zhang, Ding, Gao, Zhu, Liu, Zhang, Chen, Chen and Yu.)
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- 2023
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40. MYC-driven U2SURP regulates alternative splicing of SAT1 to promote triple-negative breast cancer progression.
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Deng L, Liao L, Zhang YL, Hu SY, Yang SY, Ma XY, Huang MY, Zhang FL, and Li DQ
- Subjects
- Animals, Humans, Mice, Cell Line, Tumor, Cell Proliferation, Eukaryotic Initiation Factor-3 genetics, Gene Expression Regulation, Neoplastic, Acetyltransferases metabolism, Alternative Splicing, Proto-Oncogene Proteins c-myc metabolism, Ribonucleoproteins metabolism, Triple Negative Breast Neoplasms pathology
- Abstract
Triple-negative breast cancer (TNBC), although highly lethal, lacks validated therapeutic targets. Here, we report that U2 snRNP-associated SURP motif-containing protein (U2SURP), a poorly defined member of the serine/arginine rich protein family, was significantly upregulated in TNBC tissues, and its high expression was associated with poor prognosis of TNBC patients. MYC, a frequently amplified oncogene in TNBC tissues, enhanced U2SURP translation through an eIF3D (eukaryotic translation initiation factor 3 subunit D)-dependent mechanism, resulting in the accumulation of U2SURP in TNBC tissues. Functional assays revealed that U2SURP played an important role in facilitating tumorigenesis and metastasis of TNBC cells both in vitro and in vivo. Intriguingly, U2SURP had no significant effects on proliferative, migratory, and invasive potential of normal mammary epithelial cells. Furthermore, we found that U2SURP promoted alternative splicing of spermidine/spermine N1-acetyltransferase 1 (SAT1) pre-mRNA by removal of intron 3, resulting in an increase in the stability of SAT1 mRNA and subsequent protein expression levels. Importantly, spliced SAT1 promoted the oncogenic properties of TNBC cells, and re-expression of SAT1 in U2SURP-depleted cells partially rescued the impaired malignant phenotypes of TNBC cells caused by U2SURP knockdown both in vitro and in mice. Collectively, these findings reveal previously unknown functional and mechanism roles of the MYC-U2SURP-SAT1 signaling axis in TNBC progression and highlight U2SURP as a potential therapy target for TNBC., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2023. Published by Elsevier B.V.)
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- 2023
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41. Performance-enhanced regenerated cellulose film by adding grape seed extract.
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Zhang YQ, Li J, Huang XJ, Yang CX, Wu C, Yang ZL, and Li DQ
- Subjects
- Food Packaging methods, Cellulose chemistry, Tensile Strength, Grape Seed Extract chemistry
- Abstract
Eco-friendly packaging material with intelligent colorimetric performance has been a requirement for food safety and quality. This work focused on a food packaging material from regenerated cellulose films that added the grape seed extract (GSE) and polyethylene glycol 200 (PEG). FTIR and SEM techniques were employed to prove the compatibility of GSE with cellulose matrix. The composite film showed an enhanced elongation at break (16.61 %) and tensile strength (33.09 MPa). The addition of PEG and GSE also improved the water contact angle of regenerated-cellulose film from 53.8° to 83.8°. Moreover, the composite films exhibited UV-blocking properties while maintaining adequate transparency. The GSE induced the regenerated films with a macroscopic change in color under different pH conditions. Furthermore, the loading of GSE slowed down the decomposition of strawberries and delayed the self-biodegradation compared with the control for more than 3 days and 18 days. The present study showed a regenerated cellulose film with acceptable mechanical and hydrophilia properties, pH-responsiveness, anti-decomposition, and delayed biodegradation performances, indicating a potential color sensor in food packaging., Competing Interests: Declaration of competing interest The authors declare no competing financial interests., (Copyright © 2023. Published by Elsevier B.V.)
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- 2023
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42. On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum .
- Author
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Qian ZM, Cheng XJ, Wang Q, Huang Q, Jin LL, Ma YF, Xie JS, and Li DQ
- Abstract
Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new online pre-column ferric ion reducing antioxidant power (FRAP)-based antioxidant reaction coupled with high performance liquid chromatography-diode array detector-quadrupole-time-of-flight mass spectrometry (HPLC-DAD-TOF/MS) was developed for rapid and high-throughput screening of natural antioxidants from three different parts of PV including stems and leaves, fruits and rhizomes. In this procedure, it was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be greatly diminished or vanish after incubating with the FRAP. The online incubation conditions including mixed ratios of sample and FRAP solution and reaction times were firstly optimized with six standards. Then, the repeatability of the screening system was evaluated by analysis of the samples of stems and leaves of PV. As a result, a total of 21 compounds mainly including flavonoids and phenolic acids were screened from the three parts of PV. In conclusion, the present study provided a simple and effective strategy to rapidly screen antioxidants in natural products., Competing Interests: All the authors declare that they have no conflicts of interest., (This journal is © The Royal Society of Chemistry.)
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- 2023
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43. Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease.
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Gu JJ, Hou YL, Yan YH, Li J, Wei YR, Ma K, Wang XQ, Zhang JH, Wang DD, Li CR, Li DQ, Sun LL, and Gao HL
- Abstract
Background: Peripheral arterial disease (PAD) has become one of the leading causes of disa-bility and death in diabetic patients. Restoring blood supply to the hindlimbs, especially by promoting arteriogenesis, is currently the most effective strategy, in which endothelial cells play an important role. Tongxinluo (TXL) has been widely used for the treatment of cardio-cerebrovascular diseases and extended for diabetes-related vascular disease., Aim: To investigate the effect of TXL on diabetic PAD and its underlying mechanisms., Methods: An animal model of diabetic PAD was established by ligating the femoral artery of db/db mice. Laser Doppler imaging and micro-computed tomography (micro-CT) were performed to assess the recovery of blood flow and arteriogenesis. Endothelial cell function related to arteriogenesis and cellular pyroptosis was assessed using histopathology, Western blot analysis, enzyme-linked immuno-sorbent assay and real-time polymerase chain reaction assays. In vitro , human vascular endothelial cells (HUVECs) and human vascular smooth muscle cells (VSMCs) were pretreated with TXL for 4 h, followed by incubation in high glucose and hypoxia conditions to induce cell injury. Then, indicators of HUVEC pyroptosis and function, HUVEC-VSMC interactions and the migration of VSMCs were measured., Results: Laser Doppler imaging and micro-CT showed that TXL restored blood flow to the hindlimbs and enhanced arteriogenesis. TXL also inhibited endothelial cell pyroptosis via the reactive oxygen species/nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3/Caspase-1/GSDMD signaling pathway. In addition, TXL restored endothelial cell functions, including maintaining the balance of vasodilation, acting as a barrier to reduce inflammation, and enhancing endothelial-smooth muscle cell interactions through the Jagged-1/Notch-1/ephrin-B2 signaling pathway. Similar results were observed in vitro ., Conclusion: TXL has a pro-arteriogenic effect in the treatment of diabetic PAD, and the mechanism may be related to the inhibition of endothelial cell pyroptosis, restoration of endothelial cell function and promotion of endothelial cell-smooth muscle cell interactions., Competing Interests: Conflict-of-interest statement: The authors declare that they have no conflicts of interest., (©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.)
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- 2023
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44. TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.
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Zhang TM, Liao L, Yang SY, Huang MY, Zhang YL, Deng L, Hu SY, Yang F, Zhang FL, Shao ZM, and Li DQ
- Subjects
- Humans, Valosin Containing Protein metabolism, Endoplasmic Reticulum-Associated Degradation, Autophagy, Signal Transduction, Apoptosis Regulatory Proteins metabolism, Membrane Proteins metabolism, Intracellular Signaling Peptides and Proteins metabolism, Triple Negative Breast Neoplasms
- Abstract
Triple-negative breast cancer (TNBC) is the most challenging breast cancer subtype to treat due to the lack of effective targeted therapies. Transmembrane (TMEM) proteins represent attractive drug targets for cancer therapy, but biological functions of most members of the TMEM family remain unknown. Here, we report for the first time that TMEM63A (transmembrane protein 63A), a poorly characterized TMEM protein with unknown functions in human cancer, functions as a novel oncogene to promote TNBC cell proliferation, migration, and invasion in vitro and xenograft tumor growth and lung metastasis in vivo . Mechanistic investigations revealed that TMEM63A localizes in endoplasmic reticulum (ER) and lysosome membranes, and interacts with VCP (valosin-containing protein) and its cofactor DERL1 (derlin 1). Furthermore, TMEM63A undergoes autophagy receptor TOLLIP-mediated autophagic degradation and is stabilized by VCP through blocking its lysosomal degradation. Strikingly, TMEM63A in turn stabilizes oncoprotein DERL1 through preventing TOLLIP-mediated autophagic degradation. Notably, pharmacological inhibition of VCP by CB-5083 or knockdown of DERL1 partially abolishes the oncogenic effects of TMEM63A on TNBC progression both in vitro and in vivo . Collectively, these findings uncover a previously unknown functional and mechanistic role for TMEM63A in TNBC progression and provide a new clue for targeting TMEM63A-driven TNBC tumors by using a VCP inhibitor. Abbreviations: ATG16L1, autophagy related 16 like 1; ATG5, autophagy related 5; ATP5F1B/ATP5B, ATP synthase F1 subunit beta; Baf-A1, bafilomycin A
1 ; CALCOCO2/NDP52, calcium binding and coiled-coil domain 2; CANX, calnexin; DERL1, derlin 1; EGFR, epidermal growth factor receptor; ER, endoplasmic reticulum; ERAD, endoplasmic reticulum-associated degradation; HSPA8, heat shock protein family A (Hsp70) member 8; IP, immunoprecipitation; LAMP2A, lysosomal associated membrane protein 2; NBR1, NBR1 autophagy cargo receptor; OPTN, optineurin; RT-qPCR, reverse transcription-quantitative PCR; SQSTM1/p62, sequestosome 1; TAX1BP1, Tax1 binding protein 1; TMEM63A, transmembrane protein 63A; TNBC, triple-negative breast cancer; TOLLIP, toll interacting protein; VCP, valosin containing protein.- Published
- 2023
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45. Destabilization of microrchidia family CW-type zinc finger 2 via the cyclin-dependent kinase 1-chaperone-mediated autophagy pathway promotes mitotic arrest and enhances cancer cellular sensitivity to microtubule-targeting agents.
- Author
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Hu SY, Qian JX, Yang SY, Andriani L, Liao L, Deng L, Huang MY, Zhang YL, Zhang FL, Shao ZM, and Li DQ
- Subjects
- Humans, CDC2 Protein Kinase genetics, CDC2 Protein Kinase metabolism, Cell Cycle Proteins metabolism, Microtubules metabolism, Mitosis genetics, Paclitaxel pharmacology, Chaperone-Mediated Autophagy, Neoplasms, Transcription Factors genetics, Transcription Factors metabolism
- Abstract
Background: Microtubule-targeing agents (MTAs), such as paclitaxel (PTX) and vincristine (VCR), kill cancer cells through activtion of the spindle assembly checkpoint (SAC) and induction of mitotic arrest, but the development of resistance poses significant clinical challenges., Methods: Immunoblotting and RT-qPCR were used to investigate potential function and related mechanism of MORC2. Flow cytometry analyses were carried out to determine cell cycle distribution and apoptosis. The effect of MORC2 on cellular sensitivity to PTX and VCR was determined by immunoblotting, flow cytometry, and colony formation assays. Immunoprecipitation assays and immunofluorescent staining were utilized to investigate protein-protein interaction and protein co-localization., Results: Here, we identified microrchidia family CW-type zinc finger 2 (MORC2), a poorly characterized oncoprotein, as a novel regulator of SAC activation, mitotic progression, and resistance of cancer cells to PTX and VCR. Mechanically, PTX and VCR activate cyclin-dependent kinase 1, which in turn induces MORC2 phosphorylation at threonine 717 (T717) and T733. Phosphorylated MORC2 enhances its interation with HSPA8 and LAMP2A, two essential components of the chaperone-mediated autophagy (CMA) mechinery, resulting in its autophagic degradation. Degradation of MORC2 during mitosis leads to SAC activation through stabilizing anaphase promoting complex/cyclosome activator protein Cdc20 and facilitating mitotic checkpoint complex assembly, thus contributing to mitotic arrest induced by PTX and VCR. Notably, knockdown of MORC2 promotes mitotic arrest induced by PTX and VCR and enhances the sensitivity of cancer cells to PTX and VCR., Conclusions: Collectively, these findings unveil a previously unrecognized function and regulatory mechanism of MORC2 in mitotic progression and resistance of cancer cells to MTAs. These results also provide a new clue for developing combined treatmentstrategy by targeting MORC2 in combination with MTAs against human cancer., (© 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.)
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- 2023
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46. Protein Phosphatase 1 Subunit PPP1R14B Stabilizes STMN1 to Promote Progression and Paclitaxel Resistance in Triple-Negative Breast Cancer.
- Author
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Liao L, Zhang YL, Deng L, Chen C, Ma XY, Andriani L, Yang SY, Hu SY, Zhang FL, Shao ZM, and Li DQ
- Subjects
- Humans, Protein Phosphatase 1 genetics, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Stathmin genetics, Stathmin metabolism, Ubiquitin Thiolesterase metabolism, Paclitaxel pharmacology, Paclitaxel therapeutic use, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms genetics, Triple Negative Breast Neoplasms pathology
- Abstract
Triple-negative breast cancer (TNBC) represents the most lethal subtype of breast cancer due to its aggressive clinical features and the lack of effective therapeutic targets. To identify novel approaches for targeting TNBC, we examined the role of protein phosphatases in TNBC progression and chemoresistance. Protein phosphatase 1 regulatory subunit 14B (PPP1R14B), a poorly defined member of the protein phosphatase 1 regulatory subunits, was aberrantly upregulated in TNBC tissues and predicted poor prognosis. PPP1R14B was degraded mainly through the ubiquitin-proteasome pathway. RPS27A recruited deubiquitinase USP9X to deubiquitinate and stabilize PPP1R14B, resulting in overexpression of PPP1R14B in TNBC tissues. Gain- and loss-of-function assays demonstrated that PPP1R14B promoted TNBC cell proliferation, colony formation, migration, invasion, and resistance to paclitaxel in vitro. PPP1R14B also induced xenograft tumor growth, lung metastasis, and paclitaxel resistance in vivo. Mechanistic investigations revealed that PPP1R14B maintained phosphorylation and stability of oncoprotein stathmin 1 (STMN1), a microtubule-destabilizing phosphoprotein critically involved in cancer progression and paclitaxel resistance, which was dependent on PP1 catalytic subunits α and γ. Importantly, the tumor-suppressive effects of PPP1R14B deficiency could be partially rescued by ectopic expression of wild-type but not phosphorylation-deficient STMN1. Moreover, PPP1R14B decreased STMN1-mediated α-tubulin acetylation, microtubule stability, and promoted cell-cycle progression, leading to resistance of TNBC cells to paclitaxel. Collectively, these findings uncover a functional and mechanistic role of PPP1R14B in TNBC progression and paclitaxel resistance, indicating PPP1R14B is a potential therapeutic target for TNBC., Significance: PPP1R14B upregulation induced by RPS27A/USP9X in TNBC increases STMN1 activity, leading to cancer progression and paclitaxel resistance., (©2022 The Authors; Published by the American Association for Cancer Research.)
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- 2023
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47. A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens.
- Author
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Hu XH, Shen S, Wu JL, Liu J, Wang H, He JX, Yao ZL, Bai YF, Zhang X, Zhu Y, Li GB, Zhao JH, You X, Xu J, Ji YP, Li DQ, Pu M, Zhao ZX, Zhou SX, Zhang JW, Huang YY, Li Y, Ning Y, Lu Y, Huang F, Wang WM, and Fan J
- Subjects
- Disease Resistance genetics, Proteasome Endopeptidase Complex metabolism, Catalase genetics, Catalase metabolism, Alleles, Hydrogen Peroxide metabolism, Plant Breeding, Plant Diseases, Gene Expression Regulation, Plant, Oryza genetics, Magnaporthe metabolism
- Abstract
Crops with broad-spectrum resistance loci are highly desirable in agricultural production because these loci often confer resistance to most races of a pathogen or multiple pathogen species. Here we discover a natural allele of proteasome maturation factor in rice, UMP1
R2115 , that confers broad-spectrum resistance to Magnaporthe oryzae, Rhizoctonia solani, Ustilaginoidea virens and Xanthomonas oryzae pv. oryzae. Mechanistically, this allele increases proteasome abundance and activity to promote the degradation of reactive oxygen species-scavenging enzymes including peroxidase and catalase upon pathogen infection, leading to elevation of H2 O2 accumulation for defence. In contrast, inhibition of proteasome function or overexpression of peroxidase/catalase-encoding genes compromises UMP1R2115 -mediated resistance. More importantly, introduction of UMP1R2115 into a disease-susceptible rice variety does not penalize grain yield while promoting disease resistance. Our work thus uncovers a broad-spectrum resistance pathway integrating de-repression of plant immunity and provides a valuable genetic resource for breeding high-yield rice with multi-disease resistance., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)- Published
- 2023
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48. Dynamic SUMOylation of MORC2 orchestrates chromatin remodelling and DNA repair in response to DNA damage and drives chemoresistance in breast cancer.
- Author
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Zhang FL, Yang SY, Liao L, Zhang TM, Zhang YL, Hu SY, Deng L, Huang MY, Andriani L, Ma XY, Shao ZM, and Li DQ
- Subjects
- Humans, Female, Chromatin Assembly and Disassembly, Drug Resistance, Neoplasm genetics, DNA Repair, DNA Damage, Chromatin, Transcription Factors metabolism, Sumoylation, Breast Neoplasms drug therapy, Breast Neoplasms genetics, Breast Neoplasms metabolism
- Abstract
Rationale: SUMOylation regulates a plethora of biological processes, and its inhibitors are currently under investigation in clinical trials as anticancer agents. Thus, identifying new targets with site-specific SUMOylation and defining their biological functions will not only provide new mechanistic insights into the SUMOylation signaling but also open an avenue for developing new strategy for cancer therapy. MORC family CW-type zinc finger 2 (MORC2) is a newly identified chromatin-remodeling enzyme with an emerging role in the DNA damage response (DDR), but its regulatory mechanism remains enigmatic. Methods: In vivo and in vitro SUMOylation assays were used to determine the SUMOylation levels of MORC2. Overexpression and knockdown of SUMO-associated enzymes were used to detect their effects on MORC2 SUMOylation. The effect of dynamic MORC2 SUMOylation on the sensitivity of breast cancer cells to chemotherapeutic drugs was examined through in vitro and in vivo functional assays. Immunoprecipitation, GST pull-down, MNase, and chromatin segregation assays were used to explore the underlying mechanisms. Results: Here, we report that MORC2 is modified by small ubiquitin-like modifier 1 (SUMO1) and SUMO2/3 at lysine 767 (K767) in a SUMO-interacting motif dependent manner. MORC2 SUMOylation is induced by SUMO E3 ligase tripartite motif containing 28 (TRIM28) and reversed by deSUMOylase sentrin-specific protease 1 (SENP1). Intriguingly, SUMOylation of MORC2 is decreased at the early stage of DNA damage induced by chemotherapeutic drugs that attenuate the interaction of MORC2 with TRIM28. MORC2 deSUMOylation induces transient chromatin relaxation to enable efficient DNA repair. At the relatively late stage of DNA damage, MORC2 SUMOylation is restored, and SUMOylated MORC2 interacts with protein kinase CSK21 (casein kinase II subunit alpha), which in turn phosphorylates DNA-PKcs (DNA-dependent protein kinase catalytic subunit), thus promoting DNA repair. Notably, expression of a SUMOylation-deficient mutant MORC2 or administration of SUMO inhibitor enhances the sensitivity of breast cancer cells to DNA-damaging chemotherapeutic drugs. Conclusions: Collectively, these findings uncover a novel regulatory mechanism of MORC2 by SUMOylation and reveal the intricate dynamics of MORC2 SUMOylation important for proper DDR. We also propose a promising strategy to sensitize MORC2-driven breast tumors to chemotherapeutic drugs by inhibition of the SUMO pathway., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)
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- 2023
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49. Resolving a nearly 90-year-old enigma: The rare Fagus chienii is conspecific with F. hayatae based on molecular and morphological evidence.
- Author
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Li DQ, Jiang L, Liang H, Zhu DH, Fan DM, Kou YX, Yang Y, and Zhang ZY
- Abstract
Taxonomic uncertainties of rare species often hinder effective prioritization for conservation. One such taxonomic uncertainty is the 90-year-old enigma of Fagus chienii . F. chienii was previously only known from the type specimens collected in 1935 in Pingwu County of Sichuan Province, China, and has long been thought to be on the verge of extinction. However, morphological similarities to closely related Fagus species have led many to question the taxonomic status of F. chienii . To clarify this taxonomic uncertainty, we used the newly collected samples to reconstruct a molecular phylogeny of Chinese Fagus species against the phylogenetic backbone of the whole genus using seven nuclear genes. In addition, we examined nine morphological characters to determine whether F. chienii is morphologically distinct from its putatively closest relatives ( F. hayatae , F . longipetiolata , and F . lucida ). Both morphological and phylogenetic analyses indicated that F. chienii is conspecific with F. hayatae . We recommended that F. chienii should not be treated as a separate species in conservation management. However, conservation strategies such as in situ protection and ex situ germplasm preservation should be adopted to prevent the peculiar " F. chienii " population from extinction., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.)
- Published
- 2023
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50. Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy.
- Author
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Yang F, Xiao Y, Ding JH, Jin X, Ma D, Li DQ, Shi JX, Huang W, Wang YP, Jiang YZ, and Shao ZM
- Subjects
- Humans, Immunotherapy methods, Ferroptosis physiology, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms pathology
- Abstract
Treatment of triple-negative breast cancer (TNBC) remains challenging. Deciphering the orchestration of metabolic pathways in regulating ferroptosis will provide new insights into TNBC therapeutic strategies. Here, we integrated the multiomics data of our large TNBC cohort (n = 465) to develop the ferroptosis atlas. We discovered that TNBCs had heterogeneous phenotypes in ferroptosis-related metabolites and metabolic pathways. The luminal androgen receptor (LAR) subtype of TNBC was characterized by the upregulation of oxidized phosphatidylethanolamines and glutathione metabolism (especially GPX4), which allowed the utilization of GPX4 inhibitors to induce ferroptosis. Furthermore, we verified that GPX4 inhibition not only induced tumor ferroptosis but also enhanced antitumor immunity. The combination of GPX4 inhibitors and anti-PD1 possessed greater therapeutic efficacy than monotherapy. Clinically, higher GPX4 expression correlated with lower cytolytic scores and worse prognosis in immunotherapy cohorts. Collectively, this study demonstrated the ferroptosis landscape of TNBC and revealed an innovative immunotherapy combination strategy for refractory LAR tumors., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
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