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In order to control the water inrush of Ⅲ638 working face floor verification borehole in Zhuzhuang Coal Mine, we first analyzed the reasons of the water inrush, and confirmed that the inrush water source was L3 limestone water, and the water channel was the floor karst development zone that had not been thoroughly controlled and JF1, JF2, JF3 faults. We proposed the idea of integrated treatment of water source and channel by using ground regional prevention and control. The treatment area is divided into water inrush risk area, fault influence area and deep weak area. Depend on “border locking + regional prevention and control”, different borehole layout schemes are proposed for different areas. Gradient pressurization grouting technology is used to reconstruct and reinforce the L3 limestone aquifer and faults. The drilled holes all meet the design requirements. After the treatment, the effect was verified by index evaluation, cross inspection and drilling. There is no floor water inrush after the treatment. The results show that the water source and channel integrated treatment technology based on ground regional prevention and control has advantages in blocking the floor water inrush.

Based on the characteristics of CO2 at high temperature, material and energy models for CO2 using in a dephosphoriza-tion converter were established to verify the feasibility of CO2 for oxidation reactions. Based on practical working conditions in a facto-ry, the effects of CO2 on the converter temperature and gas were studied. It is discovered that the CO2 ratio can be controlled within 28%, the oxygen consumption can be reduced by 16%, and the CO ratio in the gas can be increased by about 12% when the scrap ratio is 8% and the utilization ratio of CO2 is 85%. Besides, as the utilization ratio of CO2 increases, the semi-steel temperature of the converter gradually decreases, and the oxygen consumption also lowers.

Background and Purpose: Tooth eruption is a complicated process regulated by the dental follicles (DF). Our recent study discovered that tooth eruption was inhibited upon injection of bleomycin into DF. However, the mechanisms were unknown.Experimental Approach: Human dental follicle cells (hDFCs) were treated by bleomycin or exogenous TGF-β1 or transfected by plasmids loading SMAD7 or shRNA targeting SMAD7, followed by osteogenesis induction assay and signalling analysis. Human fresh DF tissues and Wistar rats were used to further confirm bleomycin function.Key Results: Bleomycin decreased expression of RUNX2 and osteogenic genes in hDFCs, reducing osteogenic capacity. TGF-β1 expression was up-regulated in bleomycin-treated hDFCs. The effects of exogenous TGF-β1 were similar to those of bleomycin in hDFCs. Additionally, compared to SMAD2/3, SMAD7 expression increased more in bleomycin- or TGF-β1-treated hDFCs. Overexpression of SMAD7 likewise significantly decreased RUNX2 expression and osteogenic capacity of hDFCs. Knockdown of SMAD7 markedly attenuated the inhibitory effects of bleomycin and TGF-β1 on osteogenic capacity and RUNX2 expression of hDFCs. Most importantly, changes in TGF-β1, SMAD7, and RUNX2 expressions were similar in the DF of rats and humans treated with bleomycin.Conclusion and Implications: SMAD7 was a negative regulator of osteogenic differentiation in DFCs through suppressing RUNX2 expression. Bleomycin or TGF-β1 inhibited osteogenic differentiation of DFCs via a TGF-β1/SMAD7/RUNX2 pathway. Our findings might be beneficial for enhancing the osteogenic activity of DFCs or inhibiting the eruption of undesirable teeth. [ABSTRACT FROM AUTHOR]