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Using the complex stratigraphic structure model, we study the changes in remaining oil on the millimeter scale in different structural parts during the different water flooding development methods. According to the actual geological structure characteristics of the oil layer, We designed and produced the meter-level experimental model, which ensures the similarity between the model structure and actual oil layer structure. The recovery rate of the primary water flooding stage is 10.36%. The stage recovery rate addition of the change flow direction stage is 7.85%. The final recovery rate is 41.36%. The physical interlayer structure has an influence on the oil saturation change in the nearby layers. The oil saturation reduction value is highest in the left part of layer 3 in the primary water flooding stage, the reduction range is 24.81%. There are 2 parts and 1 part where the oil saturation decreases by more than 10.0% in the second boost flooding stage and the change flow direction stage, respectively.

The article of record as published may be found at https://doi.org/10.1002/bmc.4478 Isaria cicadae is one of the fungi used in traditional Chinese medicine with the longest tradition. It is used not only as a herbal medicine but also as a health food in Asia, together with cultured cordyceps and mycelia of the fungus used as substitute. However, the differences in their metabolite are unknown. Using a high‐performance liquid chromatography–mass spectrometry (HPLC–MS)‐based metabolomic method, we found that the fungus varies in its metabolism during growth on wild insects, artificially raised insects and artificial medium. There were 109 discriminatory metabolites detected in the samples by orthogonal projection to latent structure discriminant analysis and one‐way ANOVA. High level of nonribosomal peptides (NRPs) only existed in the insect portions of the wild cordyceps (WI) and cultured cordyceps (CI), revealing that immunostimulation of the host insects enhanced the synthesis of NRPs in the fungus. The finding of a signifi- cantly higher level of sphingolipids in both the insect portions (WI, CI) and the coremia of the wild cordyceps (WC) and cultured cordyceps (CC) but not in cultured mycelia (CM) of I. cicadae implies that the immunostimulation of the live insects can induce the fungus to produce more sphingolipids, and this enhanced ability is probably heritable. Apart from NRPs and sphingolipids, the insect portions also contained higher levels of bioactive compounds such as lateritin, anisomycin, streptimidone and ustiloxins. In contrast, the coremium groups (WC, CC) and CM contained 10‐fold less NRP but much higher levels of sanative metabolites such as tocotrienol, 3′‐deoxy‐ hanasanagin, γ‐aminobutyric acid and phospholipids than the insect portions. The significantly higher content of antioxidants in WC, CC and CM than in WI and CI suggests that environmental oxygen has a significant effect on the metabolites. The temperature stress which the wild cordyceps encounters during growth is responsible for the relatively high content of trehalose. These findings indicate that the immunity of the host insect and growth environment have a strong impact on the metabolomic variation in Isaria cicadae. The variation in metabolites suggests differential utilization value for the insect portions, coremia and mycelia of the fungus. National Natural Science Foundation of China, Grant/Award Numbers: 31471821 and 31570024; Natural Science Foundation of Anhui Province, Grant/Award Numbers: 1808085MC89 and KJ2015ZD21 National Natural Science Foundation of China, Grant/Award Numbers: 31471821 and 31570024; Natural Science Foundation of Anhui Province, Grant/Award Numbers: 1808085MC89 and KJ2015ZD21