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12 results on '"Li, Yunpan"'

4. Live birth of chimeric monkey with high contribution from embryonic stem cells

Author

Cao, Jing, Li, Wenjuan, Li, Jie, Mazid, Md. Abdul, Li, Chunyang, Jiang, Yu, Jia, Wenqi, Wu, Liang, Liao, Zhaodi, Sun, Shiyu, Song, Weixiang, Fu, Jiqiang, Wang, Yan, Lu, Yong, Xu, Yuting, Nie, Yanhong, Bian, Xinyan, Gao, Changshan, Zhang, Xiaotong, Zhang, Liansheng, Shang, Shenshen, Li, Yunpan, Fu, Lixin, Liu, Hao, Lai, Junjian, Wang, Yang, Yuan, Yue, Jin, Xin, Li, Yan, Liu, Chuanyu, Lai, Yiwei, Shi, Xuyang, Maxwell, Patrick H., Xu, Xun, Liu, Longqi, Poo, Muming, Wang, Xiaolong, Sun, Qiang, Esteban, Miguel A., and Liu, Zhen

Published
2023
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5. Rolling back human pluripotent stem cells to an eight-cell embryo-like stage

Author

Mazid, Md. Abdul, Ward, Carl, Luo, Zhiwei, Liu, Chuanyu, Li, Yunpan, Lai, Yiwei, Wu, Liang, Li, Jinxiu, Jia, Wenqi, Jiang, Yu, Liu, Hao, Fu, Lixin, Yang, Yueli, Ibañez, David P., Lai, Junjian, Wei, Xiaoyu, An, Juan, Guo, Pengcheng, Yuan, Yue, Deng, Qiuting, Wang, Yang, Liu, Ying, Gao, Fei, Wang, Junwen, Zaman, Shahriar, Qin, Baoming, Wu, Guangming, Maxwell, Patrick H., Xu, Xun, Liu, Longqi, Li, Wenjuan, and Esteban, Miguel A.

Published
2022
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6. β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus

Author

Zhang, Meng, Lai, Yiwei, Krupalnik, Vladislav, Guo, Pengcheng, Guo, Xiangpeng, Zhou, Jianguo, Xu, Yan, Yu, Zhijun, Liu, Longqi, Jiang, Ao, Li, Wenjuan, Abdul, Mazid Md, Ma, Gang, Li, Na, Fu, Xiuling, Lv, Yuan, Jiang, Mengling, Tariq, Muqddas, Kanwal, Shahzina, Liu, Hao, Xu, Xueting, Zhang, Hui, Huang, Yinghua, Wang, Lulu, Chen, Shuhan, Babarinde, Isaac A., Luo, Zhiwei, Wang, Dongye, Zhou, Tiantian, Ward, Carl, He, Minghui, Ibañez, David P., Li, Yunpan, Zhou, Jiajian, Yuan, Jie, Feng, Yayan, Arumugam, Karthik, Di Vicino, Umberto, Bao, Xichen, Wu, Guangming, Schambach, Axel, Wang, Huating, Sun, Hao, Gao, Fei, Qin, Baoming, Hutchins, Andrew P., Doble, Bradley W., Hartmann, Christine, Cosma, Maria Pia, Qin, Yan, Xu, Guo Liang, Chen, Runsheng, Volpe, Giacomo, Chen, Liang, Hanna, Jacob H., Esteban, Miguel A., Zhang, Meng, Lai, Yiwei, Krupalnik, Vladislav, Guo, Pengcheng, Guo, Xiangpeng, Zhou, Jianguo, Xu, Yan, Yu, Zhijun, Liu, Longqi, Jiang, Ao, Li, Wenjuan, Abdul, Mazid Md, Ma, Gang, Li, Na, Fu, Xiuling, Lv, Yuan, Jiang, Mengling, Tariq, Muqddas, Kanwal, Shahzina, Liu, Hao, Xu, Xueting, Zhang, Hui, Huang, Yinghua, Wang, Lulu, Chen, Shuhan, Babarinde, Isaac A., Luo, Zhiwei, Wang, Dongye, Zhou, Tiantian, Ward, Carl, He, Minghui, Ibañez, David P., Li, Yunpan, Zhou, Jiajian, Yuan, Jie, Feng, Yayan, Arumugam, Karthik, Di Vicino, Umberto, Bao, Xichen, Wu, Guangming, Schambach, Axel, Wang, Huating, Sun, Hao, Gao, Fei, Qin, Baoming, Hutchins, Andrew P., Doble, Bradley W., Hartmann, Christine, Cosma, Maria Pia, Qin, Yan, Xu, Guo Liang, Chen, Runsheng, Volpe, Giacomo, Chen, Liang, Hanna, Jacob H., and Esteban, Miguel A.

Abstract

Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that β-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by β-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/ adaptation upon network perturbation in other contexts.

Published
2020

7. β-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus

Author

Zhang, Meng, primary, Lai, Yiwei, additional, Krupalnik, Vladislav, additional, Guo, Pengcheng, additional, Guo, Xiangpeng, additional, Zhou, Jianguo, additional, Xu, Yan, additional, Yu, Zhijun, additional, Liu, Longqi, additional, Jiang, Ao, additional, Li, Wenjuan, additional, Abdul, Mazid Md., additional, Ma, Gang, additional, Li, Na, additional, Fu, Xiuling, additional, Lv, Yuan, additional, Jiang, Mengling, additional, Tariq, Muqddas, additional, Kanwal, Shahzina, additional, Liu, Hao, additional, Xu, Xueting, additional, Zhang, Hui, additional, Huang, Yinghua, additional, Wang, Lulu, additional, Chen, Shuhan, additional, Babarinde, Isaac A., additional, Luo, Zhiwei, additional, Wang, Dongye, additional, Zhou, Tiantian, additional, Ward, Carl, additional, He, Minghui, additional, Ibañez, David P., additional, Li, Yunpan, additional, Zhou, Jiajian, additional, Yuan, Jie, additional, Feng, Yayan, additional, Arumugam, Karthik, additional, Di Vicino, Umberto, additional, Bao, Xichen, additional, Wu, Guangming, additional, Schambach, Axel, additional, Wang, Huating, additional, Sun, Hao, additional, Gao, Fei, additional, Qin, Baoming, additional, Hutchins, Andrew P., additional, Doble, Bradley W., additional, Hartmann, Christine, additional, Cosma, Maria Pia, additional, Qin, Yan, additional, Xu, Guo-Liang, additional, Chen, Runsheng, additional, Volpe, Giacomo, additional, Chen, Liang, additional, Hanna, Jacob H., additional, and Esteban, Miguel A., additional

Published
2020
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8. Generation of an induced pluripotent stem cell line (GIBHi004-A) from a Parkinson’s disease patient with mutant DJ-1/PARK7 (p.L10P)

Author

Li, Yunpan, primary, Ibañez, David P., additional, Fan, Wenxia, additional, Zhao, Ping, additional, Chen, Shuhan, additional, Md. Abdul, Mazid, additional, Jiang, Yu, additional, Fu, Lixin, additional, Luo, Zhiwei, additional, Liu, Zhenhua, additional, Yang, Yueli, additional, Guo, Jifeng, additional, Volpe, Giacomo, additional, Kanwal, Shahzina, additional, Wang, Dongye, additional, Tang, Beisha, additional, and Li, Wenjuan, additional

Published
2020
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10. Generation of an induced pluripotent stem cell line (GIBHi003-A) from a Parkinson's disease patient with mutant PINK1 (p. I368N)

Author

Abdul, Mazid Md., primary, Ibañez, David P., additional, Zhao, Ping, additional, Liu, Hao, additional, Zhong, Xiaofen, additional, Li, Yingying, additional, Zhang, Meng, additional, Li, Wenjuan, additional, Li, Yunpan, additional, Ward, Carl, additional, Chen, Shuhan, additional, Wang, Dongye, additional, Qin, Baoming, additional, Esteban, Miguel A., additional, Wang, Xiwei, additional, Fan, Wenxia, additional, and Luo, Zhiwei, additional

Published
2019
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11. Generation of a PARK2 homozygous knockout induced pluripotent stem cell line (GIBHi002-A-1) with two common isoforms abolished

Author

Zhang, Meng, primary, Ibañez, David P., additional, Fan, Wenxia, additional, Liu, Hao, additional, Zhong, Xiaofen, additional, Wang, Xiwei, additional, Li, Yingying, additional, Md. Abdul, Mazid, additional, Li, Wenjuan, additional, Li, Yunpan, additional, Ward, Carl, additional, Chen, Shuhan, additional, Wang, Dongye, additional, Qin, Baoming, additional, Esteban, Miguel A., additional, Zhao, Ping, additional, and Luo, Zhiwei, additional

Published
2019
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12. Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation.

Author

Wang J, Xie W, Li N, Li W, Zhang Z, Fan N, Ouyang Z, Zhao Y, Lai C, Li H, Chen M, Quan L, Li Y, Jiang Y, Jia W, Fu L, Mazid MA, Zhu Y, Maxwell PH, Pan G, Esteban MA, Dai Z, and Lai L

Subjects
Humans, Swine, Animals, Mesonephros, Embryo, Mammalian, Blastocyst, Mammals, Homeodomain Proteins, Induced Pluripotent Stem Cells, Pluripotent Stem Cells
Abstract

Heterologous organ transplantation is an effective way of replacing organ function but is limited by severe organ shortage. Although generating human organs in other large mammals through embryo complementation would be a groundbreaking solution, it faces many challenges, especially the poor integration of human cells into the recipient tissues. To produce human cells with superior intra-niche competitiveness, we combined optimized pluripotent stem cell culture conditions with the inducible overexpression of two pro-survival genes (MYCN and BCL2). The resulting cells had substantially enhanced viability in the xeno-environment of interspecies chimeric blastocyst and successfully formed organized human-pig chimeric middle-stage kidney (mesonephros) structures up to embryonic day 28 inside nephric-defective pig embryos lacking SIX1 and SALL1. Our findings demonstrate proof of principle of the possibility of generating a humanized primordial organ in organogenesis-disabled pigs, opening an exciting avenue for regenerative medicine and an artificial window for studying human kidney development., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Published
2023
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