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This paper interrogates the relationship between conventional planning and the emerging practice of urban experimentation through the lens of megaprojects. Focused on a low-carbon city project in Shenzhen, China, it illustrates how the project's large spatial-temporal scale facilitates a process of in-situ upscaling of planning innovation. This process involves iteratively trialing and refining novel practices through spatial demarcation and temporal phasing. This mutual constitution of megaprojects and experimentation highlights an urgent need to incorporate spatial and scale perspectives into studying and practicing urban experiments. This paper also highlights the re-negotiated planning paradox between control and flexibility under the experimental paradigm. [ABSTRACT FROM AUTHOR]

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The human genome is pervasively transcribed and produces a wide variety of long non-coding RNAs (lncRNAs), constituting the majority of transcripts across human cell types. Some specific nuclear lncRNAs have been shown to be important regulatory components acting locally. As RNA-chromatin interaction and Hi-C chromatin conformation data showed that chromatin interactions of nuclear lncRNAs are determined by the local chromatin 3D conformation, we used Hi-C data to identify potential target genes of lncRNAs. RNA-protein interaction data suggested that nuclear lncRNAs act as scaffolds to recruit regulatory proteins to target promoters and enhancers. Nuclear lncRNAs may therefore play a role in directing regulatory factors to locations spatially close to the lncRNA gene. We provide the analysis results through an interactive visualization web portal at https://fantom.gsc.riken.jp/zenbu/reports/#F6_3D_lncRNA. [ABSTRACT FROM AUTHOR]

The imaginary significance of cities to address the climate change crisis seems to have no ceiling. But the outcomes of urban climate governance have fallen short of the high promise placed on cities to contribute climate solutions. This research examines the widely observed gap between the highly expected role of cities in climate control and the disappointing reality from a perspective of the environmental state. Through a comparative study of two low-carbon city (LCC) experiments in Shenzhen and Huizhou, as well as an analysis of nation-wide administrative legal disputes related to decarbonization issues, this paper identifies an interesting legitimacy space created and manipulated locally along with the emergence of a carbon-focused environmental nation-state. Four rationales, namely carbon re-regulation, carbon formalization, carbon rationalization, and carbon exceptionalism, are identified underpinning different urban actors' articulation and leverage of the national priority of decarbonization to advance their respective agendas. Success or failure of urban climate experiments is found to be contingent upon the effective management and mediation of different rationales, giving rise to a distinctive process of "localizing the low-carbon state." The findings of this research shed light on the multifaceted effects of the greening state on the urban politics of climate change. [ABSTRACT FROM AUTHOR]

Silver nanoparticles (AgNPs) are widely used in many commercial and medical products. Serious concerns are paid on their adverse potentials to the environment and human health. In this study, toxic effects and oxidative stress induced by AgNPs with different sizes and coatings (20 nm AgNPs, 20 nm polyvinylpyrrolidone (PVP) -AgNPs and 50 nm AgNPs) in Caenorhabditis elegans (C. elegans) were investigated. The toxic effects including the shortened lifespan and decreased frequency of head thrashes and body bends of C. elegans were induced in a dose-dependent manner by AgNPs. The reactive oxygen species (ROS) production and the oxidative stress-related indicators including malondialdehyde (MDA) and glutathione (GSH) in nematodes were changed after exposure to three kinds of AgNPs. These effects were the most obvious in a 20 nm PVP-AgNPs exposure group. AgNPs could also induce the expression of genes related to oxidative stress in nematodes. In addition, the up-regulation of mtl-1 and mtl-2 in nematodes might reduce the oxidative damage caused by AgNPs, by using transgenic strains CF2222 and CL2120 nematodes. Metallothionein (MT), an antioxidant, could relieve the oxidative damage caused by AgNPs. These results suggested that 20 nm PVP-AgNPs with a smaller particle size and better dispersion have stronger toxic effects and the oxidative damage to nematodes. Mtl-1 and mtl-2 might be involved in alleviating the oxidative damage caused by AgNPs. Our findings provide clues for the safety evaluation and mechanism information of metal nanoparticles. Graphical Abstract [ABSTRACT FROM AUTHOR]

Recombinant human metallothionein III (rh‐MT‐III) is a genetically engineered product produced by Escherichia coli fermentation technology. Its molecules contain abundant reducing sulfhydryl groups, which possess the ability to bind heavy metal ions. The present study was to evaluate the binding effects of rh‐MT‐III against copper and cadmium in vitro and to investigate the antioxidant activity of rh‐MT‐III using Caenorhabditis elegans in vivo. For in vitro experiments, the binding rates of copper and cadmium were 91.4% and 97.3% for rh‐MT‐III at a dosage of 200 μg/mL at 10 h, respectively. For in vivo assays, the oxidative stress induced by copper (CuSO4, 10 μg/mL) and cadmium (CdCl2, 10 μg/mL) was significantly reduced after 72 h of exposure to different doses of rh‐MT‐III (5–500 μg/mL), indicated by restoring locomotion behavior and growth, and reducing malondialdehyde and reactive oxygen species levels in C. elegans. Moreover, rh‐MT‐III decreased the deposition of lipofuscin and fat content, which could delay the progression of aging. In addition, rh‐MT‐III (500 μg/mL) promoted the up‐regulation of Mtl‐1 and Mtl‐2 gene expression in C. elegans, which could enhance the resistance to oxidative stress by increasing the enzymatic activity of antioxidant defense system and scavenging free radicals. The results indicated that supplemental rh‐MT‐III could effectively protect C. elegans from heavy metal stress, providing an experimental basis for the future application and development of rh‐MT‐III. Rh‐MT‐III is a genetically engineered product fermented by Escherichia coli. This study evaluated the binding effects of rh‐MT‐III against copper and cadmium and the antioxidant activity of rh‐MT‐III in Caenorhabditis elegans. The data showed that rh‐MT‐III had high affinity for copper and cadmium and enhanced the stress resistance of nematodes by increasing the enzymatic activity of antioxidant defense system and scavenging free radicals. The results indicated that rh‐MT‐III could be used as an antioxidant to protect organisms from oxidative damage. [ABSTRACT FROM AUTHOR]