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In response to escalating global climate change and its increasing impacts worldwide, this study investigates the consequences of extreme weather events, focusing on the unprecedented 2022 monsoon season in the Swat River basin of Pakistan. Record-breaking rainfall, exceeding the 1960–2021 averages by 7–8 %, triggered catastrophic debris flows and floods, aggravating low-income communities' challenges. The resulting financial instability severely affected millions, causing extensive damage to homes, crops, and transportation. The study employs a multidisciplinary approach, combining field investigations, remote sensing data interpretation, and numerical simulations to identify the factors contributing to debris flow incidents. Analysis of land cover changes reveals a decrease in grasslands and an increase in barren land, indicating the adverse effects of deforestation on the region. Topography and gully morphology are crucial in initiating debris flows, with steep gradients and shallow slope failures predominant. Numerical simulations show that debris flows reached high velocities of 18 m/s and depths of 40 m within 45 minutes. Two debris flows resulted in the formation of dams along the Swat River, intensifying subsequent floods. The study emphasizes the interplay of extreme rainfall and deforestation during the rainy season, rendering the region susceptible to debris flows and hindering restoration efforts. The findings underscore the urgent need for comprehensive disaster mitigation strategies. Recommendations include climate change mitigation, reforestation initiatives, and discouraging construction activities in flood-prone and debris flow-prone regions. The study advocates for enhanced early warning systems and rigorous land use planning to protect the environment and local communities, highlighting the imperative of proactive measures in the face of escalating climate challenges. Additionally, the study investigates the spatial distribution of various events and their consequences, including potential hydro-meteorological triggers, and how such events initiate processes that change mountain landscapes. It also assesses the degree to which the 2022 monsoon can be classified as abnormal. The combination of empirical evidence and practical insights presented in this study highlights research gaps and proposes routes toward attaining a comprehensive comprehension of monsoon-triggered geological hazards and consequences. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the differences in the anti-oxidant capabilities and related gene expressions of six-month-old Hu sheep with different testis sizes. A total of 201 Hu ram lambs were fed up to 6 months in the same environment. Based on their testis weight and sperm count, 18 individuals were selected and divided into large (n = 9) and small (n = 9) groups, with an average testis weight of 158.67 g ± 5.21 g and 44.58 g ± 4.14 g, respectively. The total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malondialdehyde (MDA) concentration in testis tissue were tested. The localization of antioxidant-related genes, GPX3 and Cu/ZnSOD in testis were detected by immunohistochemistry. The GPX3, Cu/ZnSOD expression, and relative mitochondrial DNA (mtDNA) copy number were detected by quantitative real-time PCR. Compared with the small group, the T-AOC (2.69 ± 0.47 vs. 1.16 ± 0.22 U/mgprot) and T-SOD (22.35 ± 2.59 vs. 9.92 ± 1.62 U/mgprot) in the large group were significantly higher, whereas the MDA (0.72 ± 0.13 vs. 1.34 ± 0.17 nM/mgprot) and relative mtDNA copy number in the large group was significantly lower (p <.05). Immunohistochemistry results indicated that the GPX3 and Cu/ZnSOD were expressed in Leydig cells and seminiferous tubule. The expressions of GPX3 and Cu/ZnSOD mRNA in the large group were significantly higher than those in the small group (p <.05). In conclusion, Cu/ZnSOD and GPX3 widely expressed in the Leydig cells and seminiferous tubule, high expression of Cu/ZnSOD and GPX3 in a large group has a higher potential in addressing oxidative stress and contribute to spermatogenesis. [ABSTRACT FROM AUTHOR]

ADP-dependent glucokinase (ADPGK) plays an important role instead of hexokinase in regulating energy metabolism via the Embden–Meyerhof–Parnas Pathway. And energy provided via glycolysis promotes testis development and spermatogenesis. In this study, 466 Hu sheep were screened for mutations in the ADPGK gene to examine the association of the ADPGK gene polymorphisms with the testis traits and spermatogenesis. The NC_056060.1: g.31295 C > T SNP was found in the 3′-UTR region, resulting in two genotypes CC and TC type with genotypic frequencies of 0.66 and 0.34, respectively. This mutation was significantly associated with testis weight, testis long circumference, testis short girth, epididymis weight, and sperm concentration (p < 0.05). Moreover, TC genotype individuals had an increased tendency in the expression of the ADPGK gene and had significant reproductive performance advantages compared with CC genotype individuals in the study. And compared with the small testes (<50 g), the ADPGK gene expression of big testes (>160 g) increased significantly. This indicates an association between the ADPGK gene and reproductive organ parameters and sperm count in selected Hu sheep breed, and this SNP may serve as an effective DNA molecular marker for marker-assisted selection in Hu sheep breeding programs. [ABSTRACT FROM AUTHOR]