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Objective: This study aims to assess the clinical effectiveness of combining mindfulness‐based stress reduction (MBSR) with exercise intervention in improving anxiety, depression, sleep quality and mood regulation in patients with non‐small cell lung cancer (NSCLC). Methods: A total of 60 patients with NSCLC who had not received surgical treatment were selected using convenience sampling and divided into an intervention group and control group, with 30 patients in each group. The control group received conventional psychological nursing care, whereas the intervention group received a combination of MBwSR and exercise therapy. Before the intervention, a questionnaire was completed to collect the basic data of the two groups. Further questionnaires were administered at 6 and 8 weeks after treatment to assess anxiety, depression, sleep quality and other items included in the five‐item Brief Symptom Rating Scale (BSRS‐5). Results: No significant differences between the intervention and control groups were identified in terms of personal and clinical characteristics (p > 0.05). No significant differences were determined in the BSRS‐5, Self‐Rating Anxiety Scale (SAS), Self‐Rating Depression Scale (SDS) or Pittsburgh Sleep Quality Index (PSQI) scores between the intervention and control groups before the intervention. However, 6 and 8 weeks after the intervention, scores were significantly lower in both groups (p < 0.001). Significant differences in the BSRS‐5, SAS, SDS and PSQI scores were identified between the two groups at different time points (p < 0.001). Conclusion: The combination of MBSR and exercise intervention demonstrated improvements in anxiety, depression, sleep quality and BSRS‐5 scores in patients with NSCLC. [ABSTRACT FROM AUTHOR]

in this paper, the commission allocation mechanism between the mobile application store and the mobile application developer is studied under the game theory. Firstly, based on the non-cooperative game theory, the paper researches the equilibrium solution of the inter-firm game under the conditions of different sales scale, and then extend the study to the circumstance of infinite game. In addition, the paper analyzes the Pareto improvement achieved by choosing cooperative strategy of strategic alliance from different parts. Finally, problem of the commission allocation problem is resolved.

The mechanism of local scour under two vibrating pipelines is investigated numerically in this research. A sediment scour model is adopted to estimate the motion of sediment. The general moving objects model, which is dynamically coupled with fluid flow, is set up to simulate the vortex-induced vibration (VIV) of the pipeline. The sediment scour model and pipeline vibration model are verified with the previous experimental results and show good agreement. Then, the coupling effects between the pipeline vibration and the local scour are investigated numerically. The effects of G/D (the ratio of the distance between the two pipelines to the diameter of the pipelines) on the local scour and the VIV of the pipeline are examined. The results indicate that the maximum scour depth under the vibrating pipelines is much larger than the scour depth under the fixed pipelines. Due to the shadowing effect of the upstream pipeline, the maximum scour depth under the upstream pipeline is deeper than that under the downstream pipeline. The pipeline vibration magnitude is closely related to the strength of the vortex that sheds behind the pipeline. The effect of G/D on the shape and strength of the vortices that shed behind the pipelines is significant. [ABSTRACT FROM AUTHOR]

Aim: To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE)., Methods: Protein profiles of human colorectal cancer SW480 cells treated with or without CAPE were analysed using a two-dimensional (2D) electrophoresis gel-based proteomics approach. After electrophoresis, the gels were stained with Coomassie brilliant blue R-250. Digital images were taken with a GS-800 Calibrated Densitometer, and image analysis was performed using PDQuest 2-D Analysis software. The altered proteins following CAPE treatment were further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The identified proteins were validated by Western blot and immunofluorescence assay., Results: CAPE induced human colorectal cancer cell apoptosis. Four up-regulated proteins and seven down-regulated proteins in colorectal cancer cells treated with CAPE were found. The identified down-regulated proteins in CAPE-treated colorectal cancer cells were Triosephosphate Isomerase (Tim), Proteasome subunit alpha 4 (PSMA4) protein, Guanine nucleotide binding protein beta, Phosphoserine aminotransferase 1 (PSAT1), PSMA1, Myosin XVIIIB and Tryptophanyl-tRNA synthetase. Notably, CAPE treatment led to the down-regulation of PSAT1 and PSMA1, two proteins that have been implicated in tumorigenesis. The identified up-regulated proteins were Annexin A4, glyceraldehyde-3-phosphate dehydrogenase, Glucosamine-6-phosphate deaminase 1 (GNPDA1), and Glutathione peroxidase (GPX-1). Based on high match scores and potential role in cell growth control, PSMA1, PSAT1, GNPDA1 and GPX-1 were further validated by Western blotting and immunofluorescence assay. PSMA1 and PSAT1 were down-regulated, while GNPDA1 and GPX-1 were up-regulated in CAPE-treated colorectal cancer cells., Conclusion: These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.

Aim: Construct eukaryotic expression plasmid of hLIGHT-Fc gene and express hLIGHT-Fc fusion protein with high biological activity., Methods: Utilizing PCR to clone the human LIGHT cDNA from a normal human activated T cells cDNA library phAD. CAD, then using SOE (splicing by overlap extension) technique to fuse CD137 signal peptide gene into LIGHT extramembrane domain encoding sequence, inserting this recombinated LIGHT cDNA with human IgGI Fc cDNA into the eukaryotic expression plasmid pcDNA3, expressed it transiently in 293T cells, and purified by recombinated protein A affinity chromatography column, at last we checked its Mr, purity and anti-tumor activity., Results: LIGHT-Fc gene's ORF is right and coincident with we expected, then its expression was confirmed by ELISA and SDS-PAGE, and we proved that the purified LIGHT-Fc protein could inhibit the growth of some cultured tumor cell lines by MTT., Conclusion: we got a purified recombinated protein of LIGHT-Fc with anti-tumor activity and the anticipated Mr, thus lay a foundation for further works on LIGHT such as its role in immune homeostasis,the mechanism of its receptors' signal transduction, et al.