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Your search keyword '"Li, Sheng-Tian"' showing total 45 results
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45 results on '"Li, Sheng-Tian"'

11. A new cell-permeable peptide allows successful allogeneic islet transplantation in mice

Author

Noguchi, Hirofumi, Matsushita, Masayuki, Okitsu, Teru, Moriwaki, Akiyoshi, Tomizawa, Kazuhito, Kang, Sunghyun, Li, Sheng-Tian, Kobayashi, Naoya, Matsumoto, Shinichi, Tanaka, Koich, Tanaka, Noriaki, and Matsui, Hideki

Abstract

Author(s): Hirofumi Noguchi [1, 2, 3]; Masayuki Matsushita (corresponding author) [1]; Teru Okitsu [2, 3]; Akiyoshi Moriwaki [1]; Kazuhito Tomizawa [1]; Sunghyun Kang [4]; Sheng-Tian Li [1]; Naoya Kobayashi [2]; [...]

Published
2004
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15. Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels

Author

Wu, Zheng, primary, Huo, Qingwei, additional, Ren, Liang, additional, Dong, Fengping, additional, Feng, Mengyang, additional, Wang, Yue, additional, Bai, Yuting, additional, Lüscher, Bernhard, additional, Li, Sheng-Tian, additional, Wang, Guan-Lei, additional, Long, Cheng, additional, Wang, Yun, additional, Wu, Gangyi, additional, and Chen, Gong, additional

Published
2019
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43. [Effect of acute stress stimulation on the seizure induction in epileptic model rats].

Author

Sun Z, Li XR, Ju JG, Wu SJ, Zhou P, Lu QC, and Li ST

Subjects
Animals, Disease Models, Animal, Epilepsy chemically induced, Lithium Chloride adverse effects, Pentylenetetrazole adverse effects, Pilocarpine adverse effects, Rats, Behavior, Animal, Epilepsy physiopathology, Seizures physiopathology, Stress, Physiological
Abstract

This study was undertaken to observe the effect of acute stress on seizure occurrence in chronic period of epileptic model rats. Lithium-pilocarpine (LiCl-PILO)-induced epileptic rat model was constructed. At the spontaneous recurrent seizure period, acute stress stimulations such as cat's urine and foot electrical shock were applied to observe the behavioral changes and seizure occurrence. The results showed that after the cat's urine stimulation, the self-directed behaviors of the epileptic model rats decreased significantly, while the risk assessment behaviors increased significantly. The seizure occurrence, however, was not observed during the 45 min after the stimulation. Applying electrical foot shocks also did not evoke seizures in epileptic model rats. On the contrast, intra-peritoneal injection of low dose of pentylenetetrazole (PTZ, 30 mg/kg) evoked seizure more efficiently, and the duration of seizure activity was extensively prolonged in epileptic model rats than that of control rats. Taken together, these results indicate that although applying stress stimulations such as cat's urine and electrical foot shock cause several behavioral changes, they are not severe enough to evoke seizure in epileptic model rats.

Published
2012

44. HIV-1 Tat inhibits long-term potentiation and attenuates spatial learning [corrected].

Author

Li ST, Matsushita M, Moriwaki A, Saheki Y, Lu YF, Tomizawa K, Wu HY, Terada H, and Matsui H

Subjects
Animals, Animals, Newborn, Behavior, Animal, Bicuculline pharmacology, Calcium metabolism, Cells, Cultured, Dizocilpine Maleate pharmacology, Drug Interactions, Electric Stimulation methods, Embryo, Mammalian, Excitatory Amino Acid Agonists pharmacology, Excitatory Amino Acid Antagonists pharmacology, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials radiation effects, GABA Antagonists pharmacology, HIV-1 physiology, Hippocampus cytology, Hippocampus metabolism, In Vitro Techniques, Injections, Intraventricular methods, Long-Term Potentiation physiology, Long-Term Potentiation radiation effects, Male, Maze Learning physiology, Mice, Microscopy, Confocal, N-Methylaspartate pharmacology, Neural Conduction drug effects, Neurons drug effects, Neurons physiology, Patch-Clamp Techniques methods, Peptides pharmacology, Spatial Behavior physiology, Time Factors, tat Gene Products, Human Immunodeficiency Virus, Gene Products, tat pharmacology, HIV-1 chemistry, Long-Term Potentiation drug effects, Maze Learning drug effects, Spatial Behavior drug effects
Abstract

Although memory deficits have been clearly documented in patients with human immunodeficiency virus type-1 (HIV-1) infection, the physiological basis of this dysfunction is poorly understood. We focused on Tat, a viral protein released from HIV-1-infected cells and investigated its effect on spatial learning in adult mice. An intracerebroventricular injection of Tat leads to attenuation of spatial learning accompanied by suppression of long-term potentiation (LTP), the cellular basis of spatial learning, in hippocampal cornu ammonis 1 pyramidal neurons. Tat facilitates extrasynaptic but not synaptic N-methyl-D-aspartate (NMDA) receptor activity. Taken together, these data provide strong evidence that the Tat pathway underlies the development of memory dysfunction in patients with HIV-1 infection and suggest a causal relationship between Tat, the facilitation of extrasynaptic NMDA receptor activity, inhibition of LTP, and attenuation of spatial learning.

Published
2004
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45. Development of p53 protein transduction therapy using membrane-permeable peptides and the application to oral cancer cells.

Author

Takenobu T, Tomizawa K, Matsushita M, Li ST, Moriwaki A, Lu YF, and Matsui H

Subjects
Active Transport, Cell Nucleus, Adenoviridae genetics, Antineoplastic Agents pharmacology, Apoptosis, Blotting, Western, Cell Division, Cell Membrane metabolism, Cell Nucleus metabolism, Cell Survival, Cisplatin pharmacology, Cyclin-Dependent Kinase Inhibitor p21, Cyclins metabolism, Genes, Reporter, Genetic Vectors, Humans, Immunohistochemistry, Microscopy, Fluorescence, Mouth Neoplasms metabolism, Peptides chemistry, Plasmids metabolism, Protein Structure, Tertiary, Recombinant Fusion Proteins metabolism, Time Factors, Tumor Suppressor Protein p53 chemistry, Gene Transfer Techniques, Mouth Neoplasms genetics, Mouth Neoplasms therapy, Tumor Suppressor Protein p53 metabolism
Abstract

Recent studies suggest that several proteins can transverse biological membranes through protein transduction. The protein transduction domains of these proteins, 10-16 residues long, have been identified as critical domains for the protein transduction. Poly-arginine peptide also has the ability of protein transduction. Here, we show that the protein delivery system using 11 poly-arginine peptides (11R) is a powerful tool for the transduction of the biologically active tumor suppressor protein, p53, to suppress the proliferation of oral cancer cells. The 11R-fused p53 proteins (11R-p53) effectively penetrated across the plasma membrane of the cancer cells and translocated into the nucleus. The proteins induced the activity of the p21/WAF promoter and inhibited the proliferation of human oral cancer cells, in which the p53 gene was mutated. The effect was equivalent to that of the adenovirus-mediated p53 gene transduction system. Moreover, 11R-p53 enhanced the cisplatin-dependent induction of apoptosis of the cells. These data suggest that this protein transduction method may become a promising cancer therapy.

Published
2002

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