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Your search keyword '"Li, Ruo-Da"' showing total 4 results
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4 results on '"Li, Ruo-Da"'

1. Pinus massoniana bark extract protects against oxidative damage in L-02 hepatic cells and mice

Author

Wang, Mei, Ma, Hong-Ling, Liu, Bing, Wang, Hong-Bin, Xie, Heng, Li, Ruo-Da, and Wang, Jin-Fa

Subjects
Pine -- Chemical properties, Pine -- Health aspects, Pine -- Research, Oxidative stress -- Research, Liver failure -- Prevention, Liver failure -- Research, Liver cells -- Physiological aspects, Liver cells -- Research, Materia medica, Vegetable -- Research, Plant extracts -- Research, Health
Published
2010

4. [Protective effect of Pinus massoniana bark extracts against cisplatin-induced nephrotoxicity in human embryonic kidney cells].

Author

Feng DR, Dai QC, Xie H, Li RD, Zheng GY, Wang JF, and Wang HB

Subjects
Animals, Antioxidants metabolism, Epithelial Cells drug effects, Epithelial Cells enzymology, Epithelial Cells metabolism, Glutathione metabolism, Glutathione Peroxidase metabolism, HEK293 Cells, Humans, Kidney enzymology, Kidney metabolism, Malondialdehyde metabolism, Mice, Plant Bark chemistry, Reactive Oxygen Species metabolism, Superoxide Dismutase metabolism, Thioredoxin-Disulfide Reductase metabolism, Cisplatin toxicity, Kidney drug effects, Pinus chemistry, Plant Extracts pharmacology, Protective Agents pharmacology
Abstract

Objective: To investigate the in vitro protective effect of Pinus massoniana bark extracts (PMBE) against cisplatin-induced nephrotoxicity in human embryonic kidney cells (HEK293), and preliminarily study its mechanism., Method: Human embryonic kidney cells (HEK293) were cultured in vitro. The MTT assay was adopted to test the effect of PMBE and cisplatin on growth of HEK293 cells, and the protective effect of PMBE on cisplatin-induced nephrotoxicity of HEK293, and then detect the intracellular reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD) and activity of thioredoxin reductase (TrxR)., Result: PMBE could promote growth of HEK293 cells at low concentrations, but generate slight nephrotoxicity at high concentration. Cisplatin could inhibit growth of HEK293 cells, increase ROS and MDA content, while reducing SOD, CAT and TrxR. The pre-protective PMBE was added to reduce cisplatin's injury to HEK293 cells, ROS, MDA and GSH content, SOD, CAT and TrxR within certain range., Conclusion: PMBE at specific concentration has the protective effect in cisplatin-induced nephrotoxicity in HEK293 cells. Its mechanism may be related to PMBE's antioxidant activity.

Published
2013

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