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Summary: Optimal root system architecture is beneficial for water‐fertilizer use efficiency, stress tolerance and yield improvement of crops. However, because of the complexity of root traits and difficulty in phenotyping deep roots, the study on mechanisms of root development is rarely reported in wheat (Triticum aestivum L.). In this study, we identified that the LBD (LATERAL ORGAN BOUNDARIES DOMAIN) gene TaMOR (MORE ROOT in wheat) determines wheat crown root initiation. The mor mutants exhibited less or even no crown root, dwarfism, less grain number and lodging caused by few roots. The observation of cross sections showed that crown root initiation is inhibited in the mor mutants. Molecular assays revealed that TaMOR interacts with the auxin response factor ARF5 to directly induce the expression of the auxin transporter gene PIN2 (PIN‐FORMED 2) in the root base to regulate crown root initiation. In addition, a 159‐bp MITE (miniature inverted‐repeat transposable element) insertion causing DNA methylation and lower expression of TaMOR‐B was identified in TaMOR‐B promoter, which is associated with lower root dry weight and shorter plant height. The results bring new light into regulation mechanisms of crown root initiation and offer a new target for the improvement of root system architecture in wheat. [ABSTRACT FROM AUTHOR]

Plant U‐box (PUB) E3 ubiquitin ligases play essential roles in response to environmental stresses and hormone signaling, but little is known about them in wheat (Triticum aestivum L.). We characterized a U‐box E3 ubiquitin ligase gene, TaPUB15, which is conserved in wheat cultivars. TaPUB15 was expressed in multiple tissues, and the expression was much higher in roots than in other tissues. Transcription of TaPUB15 was induced by salt, abscisic acid, 4°C, and polyethylene glycol (PEG). Overexpression of TaPUB15‐D led to improve salt tolerance in transgenic rice, along with more roots in transgenic rice. Ion flux assays showed that transgenic rice plants had improved salt tolerance and maintained low Na+/K+ ratios under salinity stress. Gene expression assays indicated that several salt stress‐related genes were significantly upregulated in transgenic rice relative to the wild type. These results from transgenic rice were also verified in transgenic Arabidopsis. Our data showed that TaPUB15 plays crucial roles in enhancing salt tolerance in crop plants and served a valuable gene resource for promoting the breeding of salt‐tolerant wheat in the future. [ABSTRACT FROM AUTHOR]

By taking the common trunk highway as the study object, local and global updating rules were redesigned to obtain the improved ant colony algorithm capacity limitation model based on the traditional ant colony algorithm. Calibrating the impedance function and the calculation parameters, comparative analysis showed that the rate of convergence increased by 39.3% and the search time was shortened by 9.2%. The selection of evacuation path of the common trunk highway was solved through the improved model. In addition, the selection process of evacuation path after the transportation emergency was simulated and the visual paths was obtained based on the traffic flow of common trunk highway from Hebei province. [ABSTRACT FROM AUTHOR]

The social force model (SFM) can be applied to characterize pedestrian dynamics in normal scenarios. However, its model of interactions among pedestrians deviates from actual scenarios to some extent. Thus, we propose an improved SFM where pedestrians consider avoiding potential conflicts in advance during the walking process. Meanwhile, the response range of potential conflicts is related to the response time and relative velocity vector. Simulation results demonstrate that the conflict avoidance force plays an important role in guiding pedestrian dynamics. Conflict avoidance makes pedestrian trajectories smoother and more realistic. Moreover, for high pedestrian density (without congestion), moderate values of response time may exist, resulting in the minimum evacuation efficiency. We hope to provide some insights into how to better model interactions among pedestrians during normal evacuation.