Search

Additional file 2: Supplementary Table S1. Oligos used in the study.

Additional file 1: Supplementary Figure 1. The expression and function of hsa_circ_0003258 in PCa. (A). The heatmap showing some of the differentially expressed circRNAs between normal and prostate cancer patient plasma samples. (B). The representative images of FISH analysis of hsa_circ_0003258 in normal and PCa tissues. (C). The protein coding potential of hsa_circ_0003258. (D). The melting curve of hsa_circ_0003258 amplified product using the divergent primers. (E-H). Transwell assay detecting the migratory capacity of PCa cells after transfecting with siRNAs or vector plasmid. (I-J). Colony formation assays and CCK8 assays were utilized to evaluate the proliferation of PCa cells after knockdown of hsa_circ_0003258 in DU145 and C4���2 cells. ns: Not Significant. Supplementary Figure 2. Hsa_circ_0003258/ miR-653-5p/Arhgap5 pathway in C4-2. (A). The heatmap of differentially expressed mRNAs in DU145/sh-nc and DU145/sh- hsa_circ_0003258 cells. Each sample was mixed in three replicates. (B). Transwell assay detecting the migratory capacity of PCa cells after SCH772984 treatment for 48 h in DU145 Lv- hsa_circ_0003258 cells. (C). The potential binding miRNAs predicted by CircInteractome database. (D). Lysates from C4���2 cells was subjected to biotinylated-hsa_circ_0003258 pull-down assay and the expression levels of hsa_circ_0003258 were measured by qRT-PCR. (E). The expression levels of the top three candidate miRNAs predicted by Circinteractome database quantified by qRT-PCR after biotinylated-hsa_circ_00032583 pull-down assay in C4���2 cells. (F). The relative expression of hsa_circ_0003258 detected by qRT-PCR after the transfection of miR-653-5p mimic or inhibitor. (G-H). The qRT-PCR and Western blot showing the ARHGAP5 mRNA and protein level change of the predicated targets after silencing or overexpression of hsa_circ_0003258. (I-J). The qRT-PCR and Western blot showing the ARHGAP5 mRNA and protein level change of the predicated targets after silencing or overexpression of miR-653-5p. ns: Not Significant; * P