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Objective. To enhance and analyze the clinical effectiveness of implementing quality nursing interventions in the clinical care of critically ill patients in respiratory medicine. Methods. Clinical data of respiratory medicine patients treated in our hospital over the years were collected and 96 patients who met the requirements of the purpose of this study and the sample inclusion criteria were selected as the study subjects from the patients treated between April 2019 and January 2020. According to the care methods received by the patients in our hospital, 48 of them who implemented conventional care were used as the control group, and another 48 patients who were given quality care interventions were used as the observation group. The data were statistically recorded and comparatively analyzed for the indicators such as nursing oxygen index, heart rate, and clinical treatment efficiency of patients in both groups. Results. Compared with the control group, patients in the observation group who received quality nursing intervention had more significant improvement in blood oxygen index and heart rate after nursing care; the clinical treatment efficiency of patients in the observation group was significantly higher than that of the control group (95.83% vs. 81.25%). The data comparison between the groups showed a significant difference, P

Aims To analyse the clinicopathological, immunohistochemical and β-catenin (CTNNB1) mutation characteristics of six cases of microcystic stromal tumour of the ovary (MCST). Methods and results Six Chinese patients with MCST who ranged in age from 29 to 69 years (mean 50 years) were included in the study. Five patients were detected with a pelvic mass during routine health examinations and one patient presented initially with opsomenorrhoea. All tumours involved the left ovary, with solid-cystic cut surface in five cases and cystic cut surface in one case. Microscopically, microcysts, solid nests and hyaline degenerated fibrous stroma were variably mixed. Immunohistochemically, the tumour cells in all cases were diffusely positive for CD10, vimentin and WT-1 and negative for α-inhibin and calretinin. β-catenin expression was observed in both the nucleus and the cytoplasm in five cases and only in the cytoplasm in one case. The results of CTNNB1 mutation analysis revealed four missense point mutations in four cases, which were c.97T>C, c.101G>A, c.110C>G and c.122C>T. Conclusions MCST shows a unique morphology with characteristic immunophenotype. β-catenin expression in the nucleus and β-catenin mutations were identified in the majority of cases, which suggests that the Wnt/β-catenin pathway may play a crucial role in the tumorigenesis of MCST.

Bioaerosols are an important subgroup of atmospheric aerosols, and microorganisms are a major component of bioaerosols. Microbial activity can affect climate change, human health and environmental processes. We developed a diacetate (FDA) hydrolysis method to measure microbial activity in bioaerosols. A large number of preliminary experiments were used to optimise the experimental conditions and guarantee the applicability of this assay to environmental bioaerosol samples. The level of microbial activity in the atmosphere in the Qingdao coastal region was measured using the FDA method in May and from September to December 2012. The microbial activity in bioaerosols varied from 10.7 to 85.8 ng/m3 sodium fluorescein, with an average of 30.2 ng/m3. The microbial activity in bioaerosols displayed monthly variations, with the highest value in May. However, the activity showed no significant correlation with temperature, relative humidity and wind speed. Thus, further study is needed to elucidate the major factors and mechanisms that affect microbial activity in the atmosphere.

The associations among cardiorespiratory endurance (CRE), body mass index (BMI) and blood pressure (BP) in children are still unclear. This study aimed to examine the relationships among CRE, BMI and BP in Chinese children. Data were derived from the 2010 Chinese National Survey on Students' Constitution and Health. An endurance run test was used to determine CRE and children were stratified into low and high/moderate CRE groups. BMI was dichotomized into non-overweight and overweight. Among overweight children aged 7-12 years, the risk of high BP (HBP) was significantly higher in the low CRE group than in the high/moderate CRE group (in boys, odds ratio=1.13, 95% confidence interval: 1.03-1.24, P=0.010; in girls, odds ratio=1.18, 95% confidence interval: 1.04-1.33, P=0.010) after adjusting for age, BMI, socioeconomic status and area of residence. However, among all children aged 13-18 years and non-overweight children aged 7-12 years, we did not observe similar results. Higher CRE is associated with lower BP. Overweight children have a significantly higher risk of HBP and low CRE may increase the risk of HBP, independently of BMI, among 7- to 12-year-old overweight children.

Background Childhood obesity has been a serious public health problem. An effective school-based physical activity (PA) intervention is still lacking in China. This study aimed to assess the effectiveness of a school-based physical activity intervention during 12 weeks on obesity and related health outcomes in school children. Methods It was a non-randomized controlled trial. Altogether 921 children aged 7 to 15 years were recruited at baseline survey. Children in the intervention group (n = 388) participated in a multi-component physical activity intervention during 12 weeks that included improvement of physical education, extracurricular physical activities for overweight/obese students, physical activities at home, and health education lectures for students and parents. Children (n = 533) in the control group participated in usual practice. Results Participants had mean age of 10.4 years, mean body mass index (BMI) of 19.59 kg/m2, and 36.8 % of them were overweight or obese at baseline survey. The change in BMI in intervention group (−0.02 ± 0.06 kg/m2) was significantly different from that in control group (0.41 ± 0.08 kg/m2). The adjusted mean difference was −0.43 kg/m2 (95% CI: −0.63 to −0.23 kg/m2, P

Octamer-binding transcription factor 4A (Oct4A), one of the three spliced variants of the class V of POU transcription factor family, is mainly expressed in the nucleus of undifferentiated cells and serves as the key regulator for the maintenance of pluripotency and self-renewal. However, its specific role in regulating pluripotency and multilineage differentiation potential of dental pulp cells (DPCs) remains unknown. To explore the effect of Oct4A on pluripotency and multilineage differentiation capability of DPCs, expression of Oct4A in human dental pulp tissue and pluripotent markers Oct4A, Sox2, c-Myc, Nanog, and Klf4 in DPCs with prolonged in vitro culture were examined by immunohistochemistry and immunofluorescent staining. Oct4A transfection rate in DPCs with lentivirus was evaluated by real-time polymerase chain reaction (PCR) and western blot. Cell proliferation, multilineage differentiation, and the expression of Oct4B1, Sox2, Nanog, Klf4, c-Myc, and Utf1 in DPCs after Oct4A transfection were detected by cell counting kit-8, Alizarin red/Oil red O staining, immunofluorescent staining, alkaline phosphatase analysis, and real-time PCR. We demonstrated that Oct4A was mainly expressed in the nucleus of odontoblasts in dental pulp tissue. Oct4A, Sox2, c-Myc, Nanog, and Klf4 were primarily located in the nucleus of DPCs at early passage (passage 1) and translocated to cytoplasm at late passage (passage 7). In DPCs with Oct4A overexpression, Oct4A, Oct4B1, Sox2, Nanog, Klf4, c-Myc, and Utf1 were significantly upregulated (p

Bioaerosol samples were collected in Qingdao coastal region during July 2009 - June 2010 to investigate the concentration and community diversity of microbes in bioaerosols. Microbe concentrations (bacteria and fungi) in marine and terrestrial bioaerosols were determined and diversity indices including Shannon-Weiner index, Simpson's index and Pielou index were calculated in this study. Monthly average concentrations of terrestrial bacteria, marine bacteria, terrestrial fungi and marine fungi were in the ranges of 12-436 CFU x m(-3), 25-561 CFU x m(-3), 0-817 CFU x m(-3) and 11-1346 CFU x m(-3), respectively. There were consistent seasonal variations of these four types of microbe, with higher concentrations in spring and summer and lowest during winter, especially in February. Compared to terrestrial microbes, marine microbes account for higher proportion to the total culturable microbes, with a percentage of 63%. The number of microbial species varied from 17 to 102, and was partially correlated with microbial concentrations, however, it did not show obvious seasonal variation. Based on the analysis of calculated diversity indices, we found that the community diversities of four types of microbe were much higher in January, November and May than in February. The community diversity varied with the season, space and different microbial species, and showed a different seasonal variation from the microbial concentration.

To investigate the expression of octamer-binding transcription factor 4A(Oct4A) in human dental pulp cells(DPC)and the effect of Oct4A on the proliferation and multiple differentiation ability of DPC.Expression of Oct4A in DPC was detected by realtime quantitative PCR(RT-qPCR). siRNA-Oct4A was constructed and transfected (50 nmol/L) into DPC with Lipofectamine(TM) RNAiMAX for 24, 48, 72, 96 and 120 h. The proliferation rate of DPC was examined using cell counting kit 8 (CCK-8) assay. The alizarin red staining was used to observe the formation of calcification nodules in DPC with 14 d of osteogenic induction, and oil red O staining to observe the formation of lipid droplet in DPC with 14 d of adipogenic induction. The expression of osteogenesis-related genes dentin sialophosphoprotein (DSPP) and adipogenesis-related genes lipoprotein lipase (LPL) was detected using RT-qPCR and Western blotting.The expression of Oct4A reached the peak in P3 DPC (2.10 ± 0.10), which was 2.10 times as much as that in P1 (P = 0.000 vs. P1 and P7), and decreased along passages. The interference efficiency of DPC transfection peaked at 72 h (69.7%). Compared with control group and negative control group (IR-siRNA), the proliferation rate and multiple differentiation ability of DPC in interference group (Oct4A-siRNA) were downregulated (P = 0.000), and DSPP and LPL in DPC from interference group significantly decreased (P = 0.000).The interference of Oct4A significantly downregulated the cell proliferation rate and multilineage differentiation capability of DPC.

To explore the influencing factors on physical endurance among Chinese primary and middle school students aged 10-17.Data was used from "2010 National Physical Fitness and Health Surveillance", from which 68 458 primary and middle school students with complete information were selected. Good physical endurance was defined as endurance running time ≤ P 25 of same gender and age, poor physical endurance as ≥ P 75 of same gender and age. Difference of endurance running time in different physical endurance groups was compared; chi-square test, logistic regression and multiple level model were used to analyze influence of region, BMI, diet, exercise and exercise willingness on physical endurance.There were 34 293 (50.0%) students of good physical endurance and 34 219 (50.0%) ones of poor physical endurance in 68 458 subjects. Percentage of good physical endurance was 53.8% (18 285/33 996) in rural students, higher than urban ones (46.3% (15 954/34 462)) (χ(2) = 384.16, P0.01). Percentage of good physical endurance was 12.2% (448/3660) in obese students, lower than normal (55.2% (28 183/51 028)), overweight (29.3% (2012/6874)) and emaciated ones (52.1% (3596/6896)) (χ(2) = 3840.08, P0.01). Percentage of good physical endurance was 52.5% (25 534/48 679) in students having breakfast everyday, higher than ones never having breakfast or 1-2 times per week (41.3% (3209/7768))(χ(2) = 438.31, P0.01). Percentage of good physical endurance was 51.7% (11 580/22 379) in students having milk everyday, slightly higher than ones never or sometimes having milk (49.2% (22 659/46 079)) (χ(2) = 39.84, P0.01). Percentage of good physical endurance was 55.3% (18 961/34 314) in students with adequate physical education class, higher than ones with inadequate class (44.7% (15 278/34 144)) (χ(2) = 756.41, P0.01). Percentage of good physical endurance was 58.6% (9484/16 195) in students with one hour exercise or more per day, higher than ones with less than one hour exercise (47.4% (24 755/52 263)) (χ(2) = 619.82, P0.01). Percentage of good physical endurance was 60.1% (19 287/32 118) in students who were "very willing" to take part in endurance running, higher than "generally willing" ones (45.4% (10 569/23 272)) and "not willing" ones (33.5% (4383/13 068)) (χ(2) = 2909.61, P0.01). Multiple level analysis revealed that risk of poor physical endurance was lower in rural students (OR = 0.80, 95%CI: 0.77-0.82) than urban ones. Risk in emaciated students (OR = 1.11, 95%CI: 1.05-1.17), overweight students (OR = 2.69, 95%CI: 2.55-2.85) and obese students (OR = 7.71, 95%CI: 6.97-8.52) was higher than normal ones. Concerning diet, risk of poor physical endurance in students never having breakfast or once to twice per week was highest (OR = 1.20, 95%CI: 1.13-1.26), followed by students having breakfast three to five times per week (OR = 1.13, 95%CI: 1.08-1.18); risk in students having one bag of milk per day was higher (OR = 1.09, 95%CI: 1.02-1.17) than ones having two bags. Concerning exercise, risk of poor physical endurance in students with inadequate physical education class (OR = 1.20, 95%CI: 1.13-1.26) and less than one hour exercise per day (OR = 1.34, 95%CI: 1.29-1.40) was higher than ones with adequate physical education class and one hour exercise or more respectively. Risk of poor physical endurance in students who were "generally willing" (OR = 1.67, 95%CI: 1.61-1.73) and "not willing" (OR = 2.72, 95%CI: 2.60-2.85) to take part in endurance running was higher than "very willing" ones.Urban and rural residence, BMI, breakfast, milk, physical education class, exercise time and exercise willingness were related to primary and middle school students'physical endurance.

To explore the association between early menarche and physical fitness among adolescent girls in China.Research material was selected from the data of "2010 National Physical Fitness and Health Survey". Probability unit regression method was used to calculate the age of 10th percentile at menarche and menarche age before the 10th percentile was defined as early menarche(9.0-11.6 years old). A total of 1072 girls with early menarche were selected. Each girl with early menarche was matched with one girl from the same urban or rural locations who hadn't achieved menarche with the age difference less than 0.1 years. A total of 1072 girls without menarche were selected. Indicators of physical fitness included 50 m running, standing broad jump, 50 m×8 shuttle running and sit-ups. Differences of physical fitness between early menarche and without menarche were analyzed using t test stratified by age and urban/rural area. The multilevel models of single dependent variable and multiple dependent variables were used to analyze association between early menarche and physical fitness.A total of 2144 students were put in this research. Among girls (11.0-11.6 years old) in urban areas, 50 m running, standing broad jump and sit-ups was (9.78 ± 0.85) s, (153.81 ± 18.59) cm, and 27.79 ± 10.25, respectively for those with early menarche, while in girls without menarche was (10.01 ± 0.90) s, (149.71 ± 18.72) cm and 26.28 ± 10.11, respectively. There were significant differences between two groups on all above variables (t values were 4.02, 3.43 and 2.31, respectively with all P values0.01). Among girls in rural, 50 m×8 shuttle-running and sit-ups was (125.22 ± 15.57) s and 24.96 ± 8.97 for those with early menarche, while it was (120.92 ± 13.06) s and 22.96 ± 9.83 for those without menarche. There were significant differences between two groups on both variables(t values were 3.89 and 2.77 with both P values0.01). In addition, 50 m×8 shuttle-running in girls (10.0-10.9 years old) with early menarche was (128.52 ± 15.74) s and it was (123.89 ± 13.50) s in girls without menarche. The difference was significant (t = 2.14, P0.05). The multilevel analysis showed that 50 m running, standing broad jump and sit-ups in girls with early menarche was 0.12 s, 3.14 cm and 1.11 higher than girls without menarche (Waldχ(2) values were 4.00, 6.22 and 4.07, respectively with all P values0.05). But 50 m×8 shuttle-running in girls with early menarche was 1.95 s less than girls without menarche (Waldχ(2) = 3.96, P0.05).Early menarche may be associated with higher speed fitness, leg power and muscle power and lower physical stamina.

To analyze the association between abdominal obesity and blood pressure among 7-10 years old Chinese children.A total of 40 495 children aged 7-10 years with complete height, weight, waist circumference and blood pressure data were chosen from the data of 2010 Chinese National Survey on Students Constitution and Health. Based on the "Reference Norms for Screening Overweight and Obesity in Chinese Children and Adolescents" developed by Working Group Obesity in China (WGOC) and the waist to height ratio reference value for children (WHtR ≤ 0.46) , the children were divided into 4 body types (normal weight, simple abdominal obesity, combined obesity and other types). High blood pressure was defined as the systolic blood pressure (SBP) and/or diastolic blood pressure (DBP) higher than the P 95 blood pressure value of 2010 national student of the same gender and age. The prevalence of simple abdominal obesity, combined obesity and high blood pressure were described. Three groups (with 2165 children in each) of children with normal weight, simple abdominal obesity, combined obesity respectively were selected from the chosen ones matched by gender, age (within ± 0.5 years old) and height (within ± 0.3 cm) at the ratio of 1: 1: 1. The value of blood pressure and prevalence of high blood pressure were described.χ(2) test, analysis of variance and multilevel models were used to analyze the relationship between obesity and blood pressure.A total of 40 475 children were selected from the database, including 20 175 boys and 20 320 girls. The prevalence of simple abdominal obesity was 6.36% (2576/40 495), the prevalence of boys and girls was 7.41% (1494/20 175) and 5.32% (1082/20 320) respectively. The prevalence of combined obesity was 16.33% (6611/40 495), the prevalence of boys and girls was 21.30% (4298/20 175) and 11.38% (2313/20 320) respectively. There were statistical differences in the body type distribution of both boys and girls (χ(2) = 869.01, P0.01). The prevalence of high blood pressure was 9.62% (3896/40 495), the prevalence of boys (10.05% (2028/20 175)) was higher than girls (9.19% (1868/20 320)) (χ(2) = 8.59, P0.01). The value of SBP and DBP among combined obesity group (boys: (103.8 ± 11.3), (64.7 ± 10.1) mm Hg (1 mm Hg = 0.133 kPa); girls: (102.9 ± 12.1), (64.5 ± 10.0) mm Hg)simple abdominal obesity group (boys: (99.5 ± 11.2), (61.6 ± 9.9) mm Hg; girls: (99.6 ± 11.4), (62.6 ± 9.3) mm Hg)normal weight group (boys: (97.4 ± 10.8), (60.5 ± 9.4) mm Hg; girls: (97.2 ± 10.8), (60.8 ± 9.4) mm Hg), and the differences were statistical significant (Fboys: 113.22, 62.05; Fgirls: 54.19, 32.31, P0.01). According to the results of multilevel model, among boys, compared with the normal weight group, the SBP of combined obesity group and simple abdominal obesity group was 6.0 and 1.8 mm Hg higher respectively (Wald χ(2): 17.55, 204.94, P0.01); the DBP was 4.0 and 0.9 mm Hg higher respectively (Wald χ(2): 6.37, 114.05, P0.05). Among girls, the SBP was 5.0 and 2.1 mm Hg higher respectively (Wald χ(2):16.47, 92.52, P0.01); the DBP was 3.5 and 1.6 mm Hg higher respectively (Wald χ(2): 12.29, 57.52, P0.01). Comparing with normal group, the risk of high SBP among boys with simple abdominal obesity was higher (OR = 1.48; 95%CI: 1.06-2.06), and both the boys (SBP: OR = 3.06; 95%CI: 2.28-4.11) ; DBP: OR = 2.72; 95%CI: 1.99-3.72) and girls (SBP: OR = 2.48; 95%CI: 1.75-3.53; DBP: OR = 2.64; 95%CI: 1.82-3.93) in combined obesity group had a higher risk of high SBP and high DBP.Simple abdominal obesity is associated with the increasing of blood pressure in children, and combined obesity has a closer ties than simple abdominal obesity.

We study $B_s^0 \to J/��f_0(980)$ decays, the quark content of $f_0(980)$ and the mixing angle of $f_0(980)$ and $��(600)$. We calculate not only the factorizable contribution in QCD facorization scheme but also the nonfactorizable hard spectator corrections in QCDF and pQCD approach. We get consistent result with the experimental data of $B_s^0 \to J/��f_0(980)$ and predict the branching ratio of $B_s^0 \to J/����$. We suggest two ways to determine $f_0-��$ mixing angle $��$. Using the experimental measured branching ratio of $B_s^0 \to J/��f_0(980) $, we can get the $f_0-��$ mixing angle $��$ with some theoretical uncertainties. We suggest another way to determine $f_0-��$ mixing angle $��$ using both of experimental measured decay branching ratios $B_s^0 \to J/��f_0(980) (��)$ to avoid theoretical uncertainties., arXiv admin note: substantial text overlap with arXiv:0707.2631

We have analyzed the $D\rightarrow ��K, KK$ decay with the naive factorization (NF), QCD factorization (QCDF)and QCD factorization including soft-gluon exchanges (QCDF+SGE). In these decay channels, the soft-gluon effects are firstly calculated with light cone QCD sum rules. Comparing the three kind approaches, we can find the calculation results have made much more improved QCD factorization (QCDF) than the naive factorization (NF), and the calculation results have also made improved QCD factorization including soft-gluon exchanges (QCDF+SGE) than the QCD factorization (QCDF) in the color-suppressed decay channels. In addition, we find the soft-gluon effects are larger than the leading order contributions, and the calculation results are close to the experimental data for the color-suppressed decay channels. In color-allowed decay channel $D^{0}\rightarrow ��^{+} K^{-}$, the soft-gluon effects are small and we should consider other power terms, such as final state interaction and annihilation effects.

We study $B\to J/����^{(\prime)}$ decays and suggest two methods to determine the $��-��^{\prime}$ mixing angle. We calculate not only the factorizable contribution in QCD facorization scheme but also the nonfactorizable hard spectator corrections in pQCD approach. We get the branching ratio of $B\to J/����$ which is consistent with recent experimental data and predict the branching ratio of $B\to J/����^{\prime}$ to be $7.59\times 10^{-6}$. Two methods for determining $��-��^{\prime}$ mixing angle are suggested in this paper. For the first method, we get the $��-��^{\prime}$ mixing angle to be about $-13.1^{\circ}$, which is in consistency with others in the literature. The second method depends on less parameters so can be used to determine the $��-��^{\prime}$ mixing angle with better accuracy but needs, as an input, the branching ratio for $B\to J/����^{\prime}$which should be measured in the near future., 16pages,4figures

We study $B_{\small (s)}\to ����$ decays in a perturbative QCD approach based on $k_T$ factorization. In this approach, we calculate factorizable and non-factorizable contributions, there are no annihilation contributions due to quark content. We get the branching ratios and polarization fractions for $B_{\small (s)}\to ����$ decays . Our predictions are consistent with the current experimental data., 14 pages,one figure