Search

Purpose: The aim of this study was to evaluate the role of a novel total meniscal implant in promoting meniscal regeneration and protecting articular cartilage in a rabbit model for 3 and 6 months. Methods: Thirty‐six New Zealand rabbits were selected and divided into poly(ɛ‐caprolactone) (PG‐Pg) scaffold group, meniscectomy group and sham group. In this study, it was investigated whether PG‐Pg scaffold can prevent articular cartilage degeneration and promote tissue degeneration, and its mechanical properties at 3 and 6 months after surgery were also explored. Result: The degree of articular cartilage degeneration was significantly lower in the PG‐Pg scaffold group than in the meniscectomy group. The number of chondrocytes increased in the PG‐Pg scaffold at 3 and 6 months, while a gradual increase in the mechanical properties of the PG‐Pg stent was observed from 6 months. Conclusion: The PG‐Pg scaffold slows down the degeneration of articular cartilage, promotes tissue regeneration and improves biomechanical properties after meniscectomy. This novel meniscus scaffold holds promise for enhancing surgical strategies and delivering superior long‐term results for individuals with severe meniscus tears. Level of Evidence: NA. [ABSTRACT FROM AUTHOR]

Electrochemical conversion of CO2 into liquid fuels such as ethanol, powered by renewable electricity, is an efficient strategy for CO2 utilization to produce high value-added products. In this work, we discovered that the primary C2+ product could be switched from gaseous ethylene to liquid ethanol by directly increasing the CO2 pressure when Cu2O@Cu with a hollow sphere morphology was adopted as the catalyst. The faradaic efficiency (FE) of ethanol reached as high as 36.6% at a low overpotential of −1.0 V vs. Ag/AgCl (−0.48 V vs. RHE) at 100 bar, which was 4.6 times higher than that of 1 bar. Moreover, faster kinetics and lower overpotential for ethanol formation were obtained at high CO2 pressures. In situ Raman spectroscopy studies at different pressures in combination with density functional theory calculations demonstrated that the *CO surface coverage was increased significantly at increased CO2 pressure, which is responsible for facilitating ethanol formation during the electrochemical CO2RR. This study provides a novel and promising strategy for the selective production of ethanol on Cu-based catalysts by facilely adjusting the CO2 pressure. [ABSTRACT FROM AUTHOR]

Protein phosphorylation is an essential post-translational modification that regulates multiple cellular processes. Due to their low stoichiometry and ionization efficiency, it is critical to efficiently enrich phosphopeptides for phosphoproteomics. Several phosphopeptide enrichment methods have been reported; however, few studies have comprehensively compared different TiO2-based phosphopeptide enrichment methods using complex proteomic samples. Here, we compared four TiO2-based phosphopeptide enrichment methods that used four non-phosphopeptide excluders (glutamic acid, lactic acid, glycolic acid, and DHB). We found that these four TiO2-based phosphopeptide enrichment methods had different enrichment specificities and that phosphopeptides enriched by the four methods had different physicochemical characteristics. More importantly, we discovered that phosphopeptides had a higher deamidation ratio than peptides from cell lysate and that phosphopeptides enriched using the glutamic acid method had a higher deamidation ratio than the other three methods. We then compared two phosphopeptide fractionation methods: ammonia- or TEA-based high pH reversed-phase (HpH-RP). We found that fewer phosphopeptides, especially multi-phosphorylated peptides, were identified using the ammonia-based method than using the TEA-based method. Therefore, the TEA-based HpH-RP fractionation method performed better than the ammonia method. In conclusion, we comprehensively evaluated different TiO2-based phosphopeptide enrichment and fractionation methods, providing a basis for selecting the proper protocols for comprehensive phosphoproteomics. [ABSTRACT FROM AUTHOR]

Iron, one of the most abundant elements on earth and an essential element for living organisms, plays a crucial role in our daily metabolism. In the field of catalysis, the development of high-performance catalysts based on less toxic iron element is also of significant importance for green chemistry and a sustainable future. To construct Fe-based heterogeneous catalysts with excellent hydrogenation performance, precise modulation of the atomic coordination structure is a key strategy for enhancing catalytic activity. In this study, we present an in-situ coating method for applying a zeolitic imidazolate framework (ZIF) onto the surface of fungal hyphae. The asymmetric coordination structure of Fe 1 -N 3 P 1 was precisely tailored by utilizing the phosphorus source from the fungus and the nitrogen source in the ZIFs. Detailed characterizations and density functional theory calculations revealed that the incorporation of ZIFs not only increased the specific surface area of catalysts, but also facilitated the dispersion of Fe 2 P nanoparticles into the Fe 1 -N 3 P 1 center, making the lowest reaction energy barrier and resulting in the best performance for nitrobenzene hydrogenation when compared to the Fe 2 P nanoparticles and clusters. This research introduces a novel design concept for constructing asymmetric monoatomic configuration based on the inherent characteristics of natural microorganisms and the exogenous porous coordination polymers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The systematic and in-depth study of phosphoproteome rely on highly reproducible and specific phosphopeptide enrichment methods. At present, a variety of enrichment methods have been developed based on different principles, and these methods often display different selectivity and specificity. It is therefore very important to select the most suitable enrichment method according to different research purposes. This review summarized the phosphopeptide enrichment based on affinity chromatography, immunoprecipitation, chemical derivatization, chromatography and other newly developed methods. The advantages and disadvantages of these methods, as well as the related optimization and improvement strategies, were discussed in detail. In addition, we also briefly summarized the progress of the combination of phosphopeptide enrichment and fractionation methods developed in recent years.

In this study, a variety of functionalized ILs with dual sites including amino acid group (AA) and basic anion (R) were synthesized to investigate the suppression and cooperation between the sites in CO 2 absorption. The basic anions selected in this study with different basicity include sulfonate (Su), carboxylate (Ac), imidazolium (Im), and indolium (Ind). These ILs ([P 66614 ] 2 [AA-R]) were applied to CO 2 absorption. The results present that CO 2 capacity increases first and then decreases later with the continuous increase in the activity of the anion site. Combined with CO 2 absorption experiments, IR and NMR spectroscopic analyses and DFT calculation demonstrate that the ability of one site to capture CO 2 would be suppressed when the activity of another site is much stronger. Thus, the cooperation of dual site-functionalized ILs and high CO 2 capacity might be achieved through balancing the two sites to be equivalent. Based on this point, [P 66614 ] 2 [5Am-iPA] was further synthesized by taking the advantage of the conjugated benzene ring. As expected, [P 66614 ] 2 [5Am-iPA] showed capacity as high as 2.38 mol CO 2 per mol IL at 30 °C and 1 bar without capacity decrease even after 10 times recycling performance of CO 2 absorption and desorption., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)