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Objective: To predict the epitopes of Rv1410c Mycobacterium tuberculosis (MTB) protein., Methods: Rv1410c MTB amino acid sequence was saved and edited using Editseq software of DNAStar package, and then Protean software was used to analyze the amino acid sequence and predict the secondary structures, B cell epitopes and T cell epitopes of Rv1410c MTB. Finally, BLAST was utilized to assess its homology with human epitopes., Results: The Rv1410c MTB protein had abundant secondary structures. It contained a higher proportion of potential B cell epitopes located at 1-10, 67-77, 129-137, 189-205, 222-235, 253-267, 296-306, 330-338, 359-367, 462-467, 494-517 amino acid residues or nearby. Theses epitopes contained beta angle structure mostly, presenting with better antigenicity, higher surface probability and flexibility. There were also several potential T cell epitopes located at 16-37, 84-102, 108-115, 137-160, 202-207, 219-222, 245-263, 321-325, 355-362, 387-391, 417-445, 486-494 amino acid residues or nearby., Conclusion: Rv1410c MTB protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes.

A novel tetraethylenepentamine (TEPA)-directed method has been successfully developed for the controlled synthesis of ZnSe particles with distinctive morphologies, including nanobelts, nanowires, and hierarchically solid/hollow spheres. These structures, self-assembled from nanobelts and nanorods, have been synthesized by adjusting the reaction parameters, such as the solvent composition, reaction temperature, and the aging time. Results reveal that the volume ratio of H2O and TEPA plays a crucial role in the final morphology of ZnSe products. The mechanisms of phase formation and morphology control of ZnSe particles are proposed and discussed in detail. The products have been characterized by means of X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy (TEM), selected area electron diffraction, high-resolution TEM, Raman spectra and luminescence spectroscopy. The as-prepared ZnSe nanoparticles display shape- and size-dependent photoluminescent optical properties. This is the first time to report preparation of complex hollow structures of ZnSe crystals with hierarchy through a simple solution-based route. This synthetic route is designed to exploit a new H2O/TEPA/N2H4H2O system possibly for the preparation of other semiconductor nanomaterials.