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BackgroundMost of the researches on continuous nursing education is from the perspective of patients， and there is a lack of studies on the impact of continuous nursing education on patient caregivers， and the care quality of caregivers is closely related to postoperative rehabilitation status of the patients.ObjectiveTo investigate the effect of continuous nursing education on anxiety relief of caregivers of patients with all-robot coronary artery bypass surgery， and to provide references for improving caregivers' anxiety and promoting patients' recovery.MethodsA total of 120 caregivers of patients with all-robot coronary artery bypass surgery at the First and Sixth Center of 301 Chinese PLA General Hospital were included from January 1， 2021 to December 31， 2022. The caregivers were randomly divided into study group and control group with 60 cases in each group by systematic random sampling method. Before the patient discharge from the hospital， all caregivers received routine nursing education. The study group received continuous nursing education for 4 weeks after patient discharged. Self-rating Anxiety Scale （SAS） was used to evaluate the two groups before and one month after discharge.ResultsOne month after discharge， SAS score in the study group was significantly lower than that in the control group ［（57.77±14.08） vs. （70.19±13.60）， t=-4.913， P

Objectives: The present work aimed to optimize the culture conditions to produce extracellular alginate-lyase by Pseudoalteromonas sp. LJ1., Methods: A bacterial alginate-lyase producing strain LJ1 was isolated from Laminaria japonica by enrichment culture technique. The strain was identified based on phenotypic characters, fatty acid compositions and 16S rRNA gene sequencing. Culture conditions were optimized to produce the extracellular alginate-lyase by the single factor and orthogonal tests., Results: Strain LJ1 was identified as Pseudoalteromonas sp.. The optimal medium components were: sodium alginate 3 g/L, (NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28 degrees C for 24 h. The enzyme exhibited maximal activity at pH 7.6, 40 degrees C and NaCl 0.3 mol/L. The enzyme activity was improved by Mg2+, and inhibited by Co2+ and Zn2+ at 1 mol/L., Conclusions: Strain LJ1 was a novel alginate-lyase producing bacterium of Pseudoalteromonas.