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Harmful algal blooms (HABs) resulting from eutrophication pose a major threat to ecosystems and human health, necessitating effective control measures. Allelochemicals have shown their importance in slowing down algal proliferation due to their proven efficacy and low ecological impacts. In this study, allelopathy tea polyphenols (TPs) and β-cyclodextrin were combined to prepare slow-release algicidal microcapsules, and the diversity of microbial community in the algal inhibition process was analyzed. Results showed that TP slow-release microcapsules had strong algicidal activity. When against Microcystis aeruginosa within 20 d, their constant inhibitory rate was up to 99% compared to the control group. Microbial diversity decreased with an increase in algae density, and the species richness and diversity of algae increased under the stress of TP slow-release microcapsules. The redundancy analysis showed that the environmental factors with impacts on the abundance and diversity of bacterial communities in descending order were dissolved oxygen, pH, and temperature. This study provides a theoretical basis for the application of TP slow-release microcapsules to actual water.

Objective To explore the association of urinary heme oxygenase 1 （HO-1） with renal damage and lupus activity in patients with systemic lupus erythematosus （SLE） and determine the value of urinary HO-1 in the diagnosis and activity estimation of lupus nephritis （LN）. Methods A total of 65 patients with confirmed SLE were assigned into two groups of LN （n = 40） and non-LN （n = 25） according to whether or not there was renal damage. Urinary HO-1 was measured by enzyme-linked immunosorbent assay （ELISA） while the level of disease activity assessed by SLE Disease Activity Score （SLEDAI）. Urinary HO-1 level and other clinical data were compared between two groups. Logistic regression was utilized for examining the risk factors of LN and receiver operating characteristic （ROC） curve plotted for determining the diagnostic efficacy of urinary HO-1 for renal damage. Results Urinary HO-1 level in patients with renal damage was significantly higher than that without renal damage [（740.42 ± 86.98）ng/L vs （679.03 ± 61.33）ng/L, P = 0.003]. Urinary HO-1 level was correlated positively with SLEDAI, serum creatinine and urinary protein while negatively with glomerular filtration rate and creatinine clearance rate. Urinary HO-1, IgG, hypocomplementemia and hematuria were independent risk factors for LN （AUC = 0.853,95%CI: 0.753-0.953）. Area under the ROC curve for HO-1 alone was 0.710. When HO-1 was 721 ng/L, Youden index reached the optimal cut-off value. Conclusion Urine HO-1 level is significantly elevated in SLE patients with renal damage and it is associated with lupus activity. And HO-1 has clinical value in determining renal damage and disease activity in SLE patients.

Objective To evaluate the effect of chronic disease health management on disease activity and renal injury in patients with lupus nephritis (LN). Methods A total of 97 LN patients diagnosed with lupus nephritis were randomizedly assigned to into two groups of chronic disease health management (n=49) and conventional management (n=48). and followed up for a median of 6 months. The disease activity index (SLEDAI), and blood and urinarye parameters and medicine compliance and living quality-of-life (SF-36) and trust in doctor physician were recordedobserved in at Month 6.Results Patients who managed by of chronic disease health management had lower disease activity compared to conventional group (P

The objective of this study is to investigate the mechanism of the modification of pea protein isolate (PPI) by transglutaminase (TG) in combination with dynamic high-pressure microfluidization (DHPM) and to clarify the applicability of the modified PPI in Pickering emulsion. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy showed that the morphology of PPI particles became more uniform after the modification. The degree of cross-linking with TG increased, and the tertiary structure of protein molecules changed. In addition, the surface hydrophobicity and endogenous fluorescence of PPI decreased, the emulsifying properties improved and the average particle size decreased. The Pickering emulsion stabilized by the modified PPI had improved stability and emulsifying capacity, and the best emulsion stability was observed at 120 MPa. TG-DHPM modification could provide a feasible method for the application of PPI in Pickering emulsion. This study provides an experimental basis for the development of high-performance Pickering emulsion systems and is of significant reference value for the functional modification of natural proteins.

In order to construct a suitable pea protein-based 3D printable meat analog system, this study explored the effects and mechanism of the addition of pea dietary fiber, gluten, gellan gum and glutamine transaminase on the 3D printing performance and product quality of mixture systems using one-factor-at-a-time and orthogonal array design methods. The results showed that the 3D printing performance, texture characteristics and product characteristics significantly changed depending on the concentration of each ingredient. Using the orthogonal array method, the optimal formulation that provided the best 3D printing performance was determined as 0.45%, 5:1, 0.4% and 1.2 U/g for pea dietary fiber concentration, ratio of pea protein to gluten, gellan gum concentration and glutamine transglutaminase dosage, respectively. After the optimization, the printing accuracy improved by 27.80%. The hardness of the printed sample after cooking was 3 612.13 g, and the chewiness was 1 540.27 g·mm. In addition, the texture characteristics were better than those of the unoptimized sample. The results of infrared spectroscopy and intermolecular force showed that the addition of the components significantly enhanced the hydrogen bond, disulfide bond and hydrophobic interaction in the system. Compared with three commercially available artificial meat samples, the optimized sample exhibited significant advantages in texture characteristics, cooking loss and water-holding capacity, which were related to the structure formed by 3D printing and the interaction among the components. This study provides theoretical guidance and a basis for the application of pea protein in 3D printed plant-based meat.

This study investigated the effects of adding different amounts of sesame oleosomes on the properties of polysaccharide edible films, and explored the application of the film incorporated with sesame oleosomes in strawberry preservation in terms of sensory evaluation and physicochemical indexes. The results showed that the extracted sesame oleosomes contained 72.3% fat, which could maintain good oxidative stability for 8 days. Adding an appropriate amount of sesame oleosomes to edible films could effectively improve the softness, water vapor barrier, solubility and elongation. However, adding excess sesame oleosomes (≥ 5.6%) decreased the mechanical properties and transparency. Taken together, the optimal proportion of oleosome addition was 1.2%. Compared with the blank and oleosome-free control groups, the oleosome-incorporated film effectively delayed the rotting and softening of strawberry fruits as well as the decrease in moisture, soluble solids and titratable acids, and extended the shelf life by at least 1 day. This study showed that a certain amount of oleosomes can be used as lipid derivatives in lipid-based edible films to extend the shelf life of fruits and vegetables.

Healthy foods are foods that have additional health benefits when compared with ordinary foods. In recent years, with the vigorous development of the healthy food industry, healthy foods have gradually entered the public eye and become a hot spot. Natural products are a class of bioactive compounds that play an important role in healthy foods, and the processing is a vital part of the development of the healthy food industry. The development of healthy foods is inseparable from the ever-changing food processing technology. Based on this, this article summarizes the high-tech processing technologies for healthy foods, comprehensively compares and analyzes the high and new technologies applied in the processing steps such as natural product production, active ingredient protection, component modification, preservation, sterilization, and product design, and provides an outlook on the future development of healthy foods by using high and new technologies.

To study the effect of high Cl- environment on the corrosion of aluminum rich phosphate coatings, the corrosion behavior of phosphate coatings in high Cl- environments was analyzed using electrochemical testing methods such as open circuit potential, potentiodynamic polarization and electrochemical impedance spectroscopy, combined with scanning electron microscopy and laser confocal microscopy. Results showed that the aluminum-rich phosphate coating had a double-layer structure. Phosphate coated Al powder inside the coating provided excellent shielding performance. With the increase of Cl- concentration in the solution, the corrosion tendency and corrosion rate increased, and the corrosion resistance of the coating decreased. Moreover, aluminum-rich phosphate coatings corroded first at cracks and defects when the concentration of Cl- was low. With the increase of Cl- concentration, the corrosion of phosphate would cause the Al powder in the outer surface and the inner of coatings to fall off, and then the corrosion pits were formed. Furthermore, the corrosion pits tended to expand outward and increase in size, accelerating the corrosion of the coating and the substrate.

Objective To explore the relevance between neutrophil-to-lymphocyte ratio (NLR) and renal injury in patients with primary Sjogren's syndrome (pSS) and evaluate the clinical application value of NLR in renal injury of pSS. Methods From January 2017 to June 2022, the relevant clinical data were retrospectively reviewed for 134 hospitalized pSS patients. Based upon the presence or absence of renal injury, they were assigned into two groups of pSS with renal injury and pSS without renal injury. NLR and other laboratory parameters were compared between two groups. Through Spearman's rank correlation analysis, the correlations were examined between NLR and renal injury parameters (creatinine, urea nitrogen, uric acid, cystatin C & glomerular filtration rate) and inflammation-related parameters (erythrocyte sedimentation rate & C-reactive protein). The risk factors of renal injury were explored by multivariate Logistic regression. Receiver operating characteristic (ROC) curve was utilized for comparing the diagnostic efficacy of NLR, renal injury parameters and inflammation-related parameters for renal injury in pSS. Results NLR was markedly elevated in renal injury group than that in renal non-injury group (P

Three-dimensional food printing (3DFP) is an efficient way of food processing in line with the future lifestyle. As a delivery system, hydrogel has become a research hotspot because of its remarkable characteristics such as directed delivery. The purpose of this study was to explore the effects of 3DFP on the structure, physical properties and functions of hydrogels containing methylcellulose (MC), chlorogenic acid (CA) and hyaluronic acid (HA) for the purpose of revealing the printability and applicability of hydrogels in 3DFP processing. Texture properties, rheological properties, microstructure, embedding rate and digestive properties of the 3D printed products were measured. The results showed that the best CA-loaded hydrogel system for 3DFP processing consisted of MC, HA and CA at a mass ratio of 8:0.5:0.5. Its printed product showed the smallest width deviation (13.40%), the highest hardness, the maximum elasticity, and the minimum adhesiveness, had compact structure and uniform porosity, was not easy to collapse, and had good supportability and the best printing moldability. 3DFP well optimized the physical structure of hydrogel without changing its chemical properties. The embedding rate of CA was 22.09 percentage points higher than that before 3D printing. In simulated gastrointestinal digestion test, the release rate of CA from the printed product was significantly higher than that of the unprinted samples, showing a good sustained release effect, and the in vitro release of CA was fitted to the Ritger-Peppas model. These results showed that the hydrogel system had good printability and applicability, and 3DFP could significantly improve the targeted release of CA loaded in hydrogel.

Temporal domination of sensations (TDS) is a temporal multidimensional dynamic sensory analysis method. This method provides the evaluators with a list of the attributes of products, and the evaluators obtain dynamic multi-attribute sensory evaluation results by selecting the perceptions they think to be dominant at each moment of tasting. In this paper, the concept and process of TDS and its application in food sensory evaluation are reviewed, and future directions in the improvement of TDS are discussed. We anticipate that this paper will provide new ideas for the development of new food products based on an understanding of the dynamic changes of sensory perception during food consumption.

Blockchain is a distributed ledger technology with multi-party consensus, traceability and tamper-proof, which provides a broad application prospect for constructing efficient, trusted and secure data sharing mechanism and optimizing business processes. However, when blockchain is in a stage of rapid development, how to realize cross-chain interaction of information and cross-chain transfer of value has become an urgent problem to be solved in the process of blockchain extension to depth. Firstly, based on the review of the existing research results, the concept of blockchain interoperability is proposed, and it is divided into five aspects: inter-chain interoperability, inter-layer interoperability, inter-fork interoperability, inter-slice interoperability and interoperability between on-chain and off-chain. Secondly, by sorting out the evolution and implementation of cross-chain operation of blockchain, and referring to the TCP/IP architecture, a cross-chain operation model is designed, and the main implementation steps are functionally described. Thirdly, in view of the current research status of cross-chain operation, four key technologies of cross-chain operation, including notary mechanism, side chain/relay, hash lock and distributed private key control, are selected for analysis. Then, combined with the technical characteristics and application scenarios, the application demonstration is highlighted, some typical cross-chain application projects are introduced, and the security of blockchain cross-chain operation is analyzed. Finally, the future development trend of blockchain interoperability and cross-chain technology is summarized and explored.

Background The family doctor team dynamics mainly refer to inter-member interaction state of the family doctor team in the process of providing services. Improving the team dynamics among family doctors can help promote the enhancement of team effectiveness. The Primary Care Team Dynamics Scale (PCTDS) developed by Sara J. Singer's team at Harvard University, which can be used to comprehensively assess the primary care team dynamics. Objective To translate the PCTDS into a Chinese version, and test the reliability and validity of the Chinese version. Methods The Brislin's model for translation, back translation and cross-cultural adaptation was utilized to translate the PCTDS into a Chinese version (PCTDS-C) strictly following the principle of scale introduction, and the PCTDS-C was revised in accordance with an email-based expert consultation and a pre-test. Then a total of 569 family doctor team members were selected from 17 cities (including Guangdong's Shenzhen, Hubei's Wuhan, Zhejiang's Hangzhou, and so on) by the convenience sampling method from November 2021 to February 2022 to attend a survey using a self-developed Demographic Questionnaire and the PCTDS-C. The critical ratio (CR) method and item-total correlation were used for item analysis. The content validity of the scale was assessed by the expert consultation. The structural validity of the scale was tested by the KMO test, Bartlett's test of sphericity, exploratory factor analysis, and confirmatory factor analysis. And the reliability of the scale was evaluated by the Cronbach's α. Results Three hundred and nine of the 569 cases (54.3%) who returned responsive questionnaires were included for analysis. The absolute CR value for each of the entries was greater than 3.000 (P

为给黄油中复配天然抗氧化剂的开发提供思路，通过DPPH法测定DPPH自由基清除率和Rancimat法测定氧化诱导时间，研究单一和复配添加α-生育酚、γ-谷维素和植物甾醇3种天然抗氧化剂对黄油氧化稳定性的影响。结果表明：3种天然抗氧化剂均能提高黄油的氧化稳定性；不同复配组合对黄油氧化稳定性的影响有差异，其中α-生育酚和γ-谷维素（125 mg/kg和1 250 mg/kg）、α-生育酚和植物甾醇（125 mg/kg和1 250 mg/kg）、γ-谷维素和植物甾醇（均为800 mg/kg）3种复配组合对黄油氧化稳定性的提升较大，特别是α-生育酚和γ-谷维素（125 mg/kg和1 250 mg/kg）组合的氧化诱导时间可提升3.70 h，DPPH自由基清除率达80.4%，氧化稳定性能优于其他复配组合。综上，将复配天然抗氧化剂添加到黄油中可提高其氧化稳定性。 To provide ideas for the development of compounded natural antioxidants in butter, DPPH radical scavenging rate was evaluated by DPPH method and oxidation induction time was analyzed by Rancimat method. The effects of single or compound addition of three natural antioxidants, α-tocopherol, γ-oryzanol and phytosterol on oxidative stability of butter were investigated. The results showed that all three natural antioxidants could enhance oxidative stability of butter, and different combinations had different effects on oxidative stability of butter. With the three combinations of α-tocopherol and γ-oryzanol (125 mg/kg and 1 250 mg/kg), α-tocopherol and phytosterol (125 mg/kg and 1 250 mg/kg), and γ-oryzanol and phytosterol (both 800 mg/kg) showed greater improvement in the oxidative stability of butter. In particular, the oxidation induction time of group α-tocopherol and γ-oryzanol (125 mg/kg and 1 250 mg/kg) could be increased by 3.70 h, and the DPPH radical scavenging rate reached 80.4%, and the oxidative stability was better than other combinations. In conclusion, the addition of the compounded natural antioxidants to butter can improve its oxidative stability.

In order to enhance the hydrophilicity and corrosion resistance of aluminum alloy surface， a hydrophilic surface was obtained on 2024 and 6061 aluminum alloy substrates by anodic oxidation，reaming and coating of sodium fluosilicate，and the surface morphology，elemental composition， wettability and electrochemical properties of the as-prepared hydrophilic surface were analyzed. Results showed that after anodizing with 15%(mass fraction) sulfuric acid solution， the oxide films of 2024 aluminum alloy and 6061 aluminum alloy presented the rough nano-particles and dispersed micropores respectively. And the oxide film of 2024 aluminum alloy could better combine with sodium fluorosilicate to form a surface film with good hydrophilic effect. These two kinds of aluminum alloys were coated in sodium fluorosilicate solution， and hydrophilic films with contact angles less than 30° were obtained. The average contact angle of 2024 aluminum alloy surface was 18.5° and both aluminum alloys obtained good hydrophilicity. The electrochemical test results showed that the corrosion resistance of 2024 and 6061 aluminum alloys both decreased after coating， and the corrosion resistance of 6061 aluminum alloy changed more obviously. In all， the hydrophilicity and corrosion resistance of 2024 aluminum alloy were better than those of 6061 aluminum alloy after coating.

Abstract Background Antineutrophil Cytoplasmic Antibodies (ANCA) associated glomerulonephritis (AGN) is a group of autoimmune diseases and mono-macrophages are involved in its glomerular injuries. In this study, we aim to investigate the role of CD206+ mono-macrophages in AGN. Methods 27 AGN patients (14 active AGN, 13 remissive AGN) together with healthy controls (n = 9), disease controls (n = 6) and kidney function adjusted controls (n = 9) from Department of Nephrology, Ruijin hospital were recruited. Flow cytometry was used to study proportion of CD206+ cells in peripheral blood. Immunohistochemistry for CD206 staining was performed and CD206 expression was scored in different kidney regions. Serum soluble CD206 (sCD206) was measured by enzyme-linked immunosorbent assay (ELISA). We also generated murine myeloperoxidase (MPO) (muMPO) ANCA by immunizing Mpo −/− mice. Mouse bone marrow-derived macrophages (BMDMs) from wild C57BL/6 mice and peripheral blood mononuclear cell (PBMC) derived macrophages from healthy donors were treated with MPO ANCA with or without its inhibitor AZD5904 to investigate the effects of MPO-ANCA on CD206 expression. Results The proportion of peripheral CD206+CD68+ cells in active AGN patients were significantly higher than that in remissive patients (p

Objective: To develop pigmented rice lactic acid beverage with good antioxidant and hypoglycemic activity. Methods: Taking black and purple rice as the main material, the effect of mass ratio of purple rice to black rice, the addition amount of Lactobacillus bacteria, fermentation time and solid-liquid ratio on the sensory evaluation value, total phenolics content, ferric ion reducing antioxidant power （FRAP）, ABTS free radical scavenging ability, and the inhibition rate of α-glucosidase of pigmented rice lactic acid beverage were explored. Results: The optimal technology of pigmented rice lactic acid beverage was as follows: the mass ratio of purple rice to black rice was 1∶5, the solid-liquid ratio was 1∶8 （g/mL）, the addition amount of lactic acid bacteria was 5%, and the fermentation time was 40 h. Under these conditions, the sensory score of pigmented rice lactic acid beverage was 83.6±2.51, ABTS radical scavenging capacity was （145.02±7.88） mmol TE/g·DW, FRAP was （33.41±1.70） mmol Fe2+/g·DW, total phenolics were （4 208.78±281.26） μg GAE/g·DW, α-glucosidase inhibitory rate activity was （13.94±0.01）%. Conclusion: The pigmented rice lactic acid beverage possessed purplish red color, soft in taste, moderate in sour and sweet taste, with good antioxidant activity and α-glucosidase inhibition effect.